Recovery Or Purification Patents (Class 435/239)
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Patent number: 8415133Abstract: The present invention relates to compositions and pharmaceutical compositions comprising poxviruses and more particularly extracellular enveloped viruses. The present invention also relates to a process for producing poxviruses and poxviruses obtained thereof. Moreover, the present invention also relates to the use of said poxvirus and said composition for the preparation of a medicament.Type: GrantFiled: October 24, 2011Date of Patent: April 9, 2013Assignee: Transgene S.A.Inventors: Daniel Malarme, Yves Cordier, Claude Sene
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Patent number: 8415132Abstract: The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.Type: GrantFiled: August 22, 2011Date of Patent: April 9, 2013Assignee: Bavarian Nordic A/SInventors: Rene Djurup, Sara Post Hansen
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Patent number: 8415134Abstract: The present invention relates to compositions and pharmaceutical compositions comprising poxviruses and more particularly extracellular enveloped viruses. The present invention also relates to a process for producing poxviruses and poxviruses obtained thereof. Moreover, the present invention also relates to the use of said poxvirus and said composition for the preparation of a medicament.Type: GrantFiled: October 24, 2011Date of Patent: April 9, 2013Assignee: Transgene S.A.Inventors: Daniel Malarme, Yves Cordier, Claude Sene
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Publication number: 20130084620Abstract: The present invention relates to compositions and pharmaceutical compositions comprising poxviruses and more particularly extracellular enveloped viruses. The present invention also relates to a process for producing poxviruses and poxviruses obtained thereof. Moreover, the present invention also relates to the use of said poxvirus and said composition for the preparation of a medicament.Type: ApplicationFiled: October 24, 2011Publication date: April 4, 2013Applicant: TRANSGENE S.A.Inventors: Daniel MALARME, Yves CORDIER, Claude SENE
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Patent number: 8409842Abstract: The present invention relates to methods and materials for recombinant adeno-associated virus production. More particularly, the invention relates to use of recombinant adenovirus encoding adeno-associated virus protein in recombinant adeno-associated virus production methods.Type: GrantFiled: March 18, 2009Date of Patent: April 2, 2013Assignee: Nationwide Children's Hospital, Inc.Inventors: Kelly Reed Clark, Philip R. Johnson, Jr.
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Patent number: 8404248Abstract: Polypeptides, polynucleotides, methods, compositions, and vaccines comprising influenza hemagglutinin and neuraminidase variants are provided.Type: GrantFiled: June 29, 2012Date of Patent: March 26, 2013Assignee: Medimmune, LLCInventors: Chin-Fen Yang, George Kemble, Chongguang Liu
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Publication number: 20130071429Abstract: The present invention provides attenuated viruses for use as vaccines and for the treatment and/or prevention of viral diseases and/or infections.Type: ApplicationFiled: January 28, 2011Publication date: March 21, 2013Applicant: UNIVERSITY COURT OF THE UNIVERSITY OF ST ANDREWSInventors: Benjamin Brennan, Richard Michael Elliott
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Publication number: 20130052165Abstract: The present invention, in some embodiments thereof, relates to methods of producing adenoviruses such as pro- and anti-angiogenic adenovirus vectors and preparations generated thereby. Particularly, in some embodiments, the viral vectors comprise a heterologous pro- or anti-angiogenic gene under the transcriptional control of the murine pre-proendothelin promoter (e.g. PPE-1-3X), for targeted expression of in angiogenic endothelium.Type: ApplicationFiled: January 12, 2011Publication date: February 28, 2013Inventors: Livnat Bangio, Naamit Sher, Eyal Breitbart
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Patent number: 8377672Abstract: Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed.Type: GrantFiled: January 7, 2011Date of Patent: February 19, 2013Assignee: 3M Innovative Properties CompanyInventors: Jerald K. Rasmussen, Catherine A. Bothof, Kannan Seshadri, Erin A. Satterwhite, Robert T. Fitzsimons, James I. Hembre, Mahfuza B. Ali
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Publication number: 20130034582Abstract: A method for rescuing a virus by reverse genetics is provided in which cells are added after transfection.Type: ApplicationFiled: October 20, 2010Publication date: February 7, 2013Applicant: NOVARTIS AGInventors: Philip Dormitzer, Björn Keiner, Pirada Suphaphiphat, Michael Franti, Peter Mason, Jennifer Uhlendorff, Mikhail Matrosovich
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Patent number: 8367391Abstract: A pretreatment method of a specimen used for detecting or determining abnormal prion protein (PrPres) associated with transmissible spongiform encephalopathy (TSE), wherein (1) a specimen which had been treated with proteinase K is heated in the presence of sodium dodecyl sulfate (SDS) to dissolve proteins and inactivate infectious activity in the specimen at the same time; (2) the specimen processed in the above (1) is cooled under a neutral condition to make abnormal prion protein (PrPres) associated with transmissible spongiform encephalopathy (TSE) aggregated; (3) the aggregate formed in the above (2) is separated from a solution; and (4) the separated PrPres aggregate is detected by the ultrasensitive chemiluminescence method.Type: GrantFiled: August 22, 2008Date of Patent: February 5, 2013Assignee: Japanese Red Cross SocietyInventor: Kazuo Tsukui
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Publication number: 20130023034Abstract: The invention relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein a nuclear localization signal (NLS) in said Parvoviral Rep protein is mutated as compared with a corresponding wild type sequence. The invention also relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein the zinc finger domain in said Parvoviral Rep protein is mutated as compared with a corresponding wild type sequence. Further, the invention relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein an amino acid at position 43, 57, 79, 97, 120, 179, 305, 484, 493 or 571 of the said Parvoviral Rep protein is mutated in comparison to a corresponding wild type sequence, said amino acid position being defined with reference to SEQ ID NO: 2.Type: ApplicationFiled: March 11, 2011Publication date: January 24, 2013Inventors: Yvet Noordman, Jacek Lubelski, Andrew Christian Bakker
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Publication number: 20130022602Abstract: The invention is directed to an improved method to manufacture virus for use in vaccine by culturing infected cells that have been modified to overexpress miR-144. The invention is also directed to manipulating the activity or level of miR-144 in subjects in order to modulate the antiviral and immune response systems.Type: ApplicationFiled: July 5, 2012Publication date: January 24, 2013Inventors: Carrie M. Rosenberger, Alan A. Aderem
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Publication number: 20120328651Abstract: The present invention provides recombinant replication-defective adenoviral vectors derived from chimpanzee adenoviruses and methods for generating recombinant adenoviruses in human E1-expressing cell lines. The invention also provides compositions and methods suitable for use for the delivery and expression of transgenes encoding immunogens against which a boosted immune response is desired. The invention further provides methods of generating clinical grade vector stocks suitable for use in humans. In a particular embodiment the invention contemplates the use of vectors comprising transgenes which encode tumor associated antigens in vaccines and pharmaceutical compositions for the prevention and treatment of cancer.Type: ApplicationFiled: June 21, 2012Publication date: December 27, 2012Inventors: Stefano Colloca, Alfredo Nicosia, Elisabetta Sporeno, Agostino Cirillo, Bruno Bruni Ercole, Annalisa Meola, Paolo Palazzolo
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Patent number: 8337860Abstract: The invention is related to a dengue virus or chimeric dengue virus that contains a mutation in the 3? untranslated region (3?-UTR) comprising a ?30 mutation that removes the TL-2 homologous structure in each of the dengue virus serotypes 1, 2, 3, and 4, and nucleotides additional to the ?30 mutation deleted from the 3?-UTR that removes sequence in the 5? direction as far as the 5? boundary of the TL-3 homologous structure in each of the dengue virus serotypes 1, 2, 3, and 4, or a replacement of the 3?-UTR of a dengue virus of a first serotype with the 3?-UTR of a dengue virus of a second serotype, optionally containing the ?30 mutation and nucleotides additional to the ?30 mutation deleted from the 3?-UTR; and immunogenic compositions, methods of inducing an immune response, and methods of producing a dengue virus or chimeric dengue virus.Type: GrantFiled: August 15, 2007Date of Patent: December 25, 2012Assignee: The United States of America, as represented by the Secretary of the Department of Health & Human ServicesInventors: Stephen S. Whitehead, Joseph E. Blaney, Brian R. Murphy
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Publication number: 20120301503Abstract: Polypeptides, polynucleotides, methods, compositions, and vaccines comprising influenza hemagglutinin and neuraminidase variants are provided.Type: ApplicationFiled: June 29, 2012Publication date: November 29, 2012Applicant: MEDIMMUNE, LLCInventors: Chin-Fen Yang, George Kemble, Chongguang Liu
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Publication number: 20120294892Abstract: The present invention relates to avirulent Salmonella Gallinarum variants by inactivating virulence gene clusters of Salmonella Gallinarum (SG), a main pathogen of avian salmonellosis, and various uses thereof notably in the production of Salmonella-specific lytic bacteriophages, pharmaceutical compositions and feed additives.Type: ApplicationFiled: October 17, 2011Publication date: November 22, 2012Applicant: CJ CHEILJEDANG CORPORATIONInventors: Hyang CHOI, Soo An Shin, Si Yong Yang, Young Wook Cho
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Patent number: 8309099Abstract: The present invention is based on the development of a dual promoter system (preferably a RNA pol I-pol II system) for the efficient intracellular synthesis of viral RNA. The resultant minimal plasmid-based system may be used to synthesize any RNA virus, preferably viruses with a negative single stranded RNA genome. The viral product of the system is produced when the plasmids of the system are introduced into a suitable host cell. One application of the system is production of attenuated, reassortant influenza viruses for use as antigens in vaccines. The reassortant viruses generated by cotransfection of plasmids may comprise genes encoding the surface glycoproteins hemagglutinin and neuraminidase from an influenza virus currently infecting the population and the internal genes from an attenuated influenza virus. An advantageous property of the present invention is its versatility; the system may be quickly and easily adapted to synthesize an attenuated version of any RNA virus.Type: GrantFiled: June 1, 2011Date of Patent: November 13, 2012Assignee: St. Jude Children's Research HospitalInventor: Erich Hoffmann
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Publication number: 20120282674Abstract: The present invention relates to a method for the preparation of a pharmaceutical composition for the prevention or/and treatment of an influenza virus infection.Type: ApplicationFiled: September 10, 2010Publication date: November 8, 2012Applicant: Maxplanck-Gesellschaft Zur Forderung der Wissenschaften E.V.Inventors: Nikolaus Machuy, Alexander Karlas, Thomas F. Meyer
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Publication number: 20120276614Abstract: The present invention relates to compositions and pharmaceutical compositions comprising poxviruses and more particularly extracellular enveloped viruses. The present invention also relates to a process for producing poxviruses and poxviruses obtained thereof. Moreover, the present invention also relates to the use of said poxvirus and said composition for the preparation of a medicament.Type: ApplicationFiled: October 24, 2011Publication date: November 1, 2012Applicant: TRANSGENE S.A.Inventors: Daniel MALARME, Yves CORDIER, Claude SENE
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Patent number: 8293498Abstract: The present invention provides methods for the generation of viable reoviruses using only cloned nucleic acid segments representing the RNA segments of the reovirus genome.Type: GrantFiled: December 19, 2007Date of Patent: October 23, 2012Assignee: Vanderbilt UniversityInventors: Terence S. Dermody, Takeshi Kobayashi, James D. Chappell
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Patent number: 8288145Abstract: The use of macrolide polyene antibiotics or derivatives or analogues thereof as culture supplement for the propagation of virus is described. Further pharmaceutical compositions comprising a virus and a macrolide polyene antibiotic or a derivative or analogue thereof and methods for using of macrolide polyene antibiotics for transfection and infection of cells as well as the use of macrolide polyene antibiotics for the isolation of virus from clinical samples are disclosed.Type: GrantFiled: October 11, 2007Date of Patent: October 16, 2012Assignee: AVIR Green Hills BiotechnologyInventors: Elisabeth Röthl, Andrej Egorov
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Publication number: 20120258136Abstract: The present invention provides novel serum-free cell culture medium and methods for cultivating MDCK cells. In particular, non-tumorigenic MDCK cells. The present invention also provides methods for producing influenza viruses (e.g., particularly cold-adapted, and/or temperature sensitive, and/or attenuated influenza viruses) that eliminate the need for a cell culture medium exchange step. The novel medium and methods are useful to grow influenza viruses, in cell culture to high titer. The present invention further provides purification methods for purifying influenza viruses with high overall recovery of live virus and result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.Type: ApplicationFiled: May 16, 2012Publication date: October 11, 2012Applicant: MEDIMMUNE, LLCInventors: Jonathan LIU, Mark Thompson, Luis J. Maranga, Floro Cataniag, Simon S. Hsu
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Publication number: 20120258475Abstract: A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.Type: ApplicationFiled: April 6, 2012Publication date: October 11, 2012Applicant: BIOCEPT, INC.Inventors: Zhongliang TANG, Ram S. BHATT, Pavel TSINBERG
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Publication number: 20120252098Abstract: The invention provides methods and materials for using apatite chromatography supports to dissociate and remove contaminants that are complexed to biological products. The invention further provides materials and methods for dissociating aggregations of target biological molecules or improperly folded target molecules to improve purification of the target molecule.Type: ApplicationFiled: September 20, 2011Publication date: October 4, 2012Applicant: Bio-Rad Laboratories, Inc.Inventor: Peter S. Gagnon
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Publication number: 20120251568Abstract: Described herein are modified influenza virus NS gene segments and nucleic acid sequences encoding such modified influenza virus NS gene segments. In certain embodiments, a modified influenza virus NS gene segment described herein comprises an influenza virus NS 1 open reading frame (ORF) lacking a stop codon, a heterologous nucleotide sequence, a 2A autoproteolytic cleavage site or another cleavage site, an NEP ORF, wherein the gene segment has one or more mutations in either the splice acceptor site, splice donor site, or both the splice acceptor and splice donor sites that prevents splicing of mRNA. Also described herein are recombinant influenza viruses comprising a modified influenza virus NS gene segment and the use of such viruses. The recombinant influenza viruses may be use in the prevention and/or treatment of influenza virus disease or as a delivery vector.Type: ApplicationFiled: July 27, 2010Publication date: October 4, 2012Inventors: Adolfo Garcia-Sastre, Peter Palese, Balaji Manicassamy
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Publication number: 20120252091Abstract: A substrate comprising a crosslinked polymer primer layer, and grafted thereto a ligand-functionalized polymer is provided. The grafted polymer has the requisite affinity for binding neutral or negatively charged biomaterials, such as cells, cell debris, bacteria, spores, viruses, nucleic acids, and proteins, at pH's near or below the pI's of the biomaterials.Type: ApplicationFiled: January 19, 2012Publication date: October 4, 2012Inventors: Jerald K. Rasmussen, Catherine A. Bothof, Kannan Seshadri, James I. Hembre, Robert T. Fitzsimons, JR., George W. Griesgraber, Yi He
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Publication number: 20120244600Abstract: Polypeptides, polynucleotides, methods, compositions, and vaccines comprising influenza hemagglutinin and neuraminidase variants are provided.Type: ApplicationFiled: December 1, 2011Publication date: September 27, 2012Applicant: MedImmune, LLCInventor: Hong JIN
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Publication number: 20120244183Abstract: Described herein are chimeric influenza virus gene segments and nucleic acid sequences encoding such chimeric influenza virus gene segments. A chimeric influenza virus gene segment described herein comprises packaging signals found in the non-coding and coding regions of one type of influenza virus gene segment and an open reading frame of a different type of influenza virus gene segment or fragment thereof. Also described herein are recombinant influenza viruses comprising two or more chimeric influenza virus gene segments and the use of such viruses in the prevention and/or treatment of influenza virus disease.Type: ApplicationFiled: July 29, 2010Publication date: September 27, 2012Inventors: Adolfo Garcia-Sastre, Peter Palese, Qinshan Gao
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Publication number: 20120237974Abstract: The invention relates to methods for the production of a recombinant protein in a mammalian cell and methods to enhance the production of recombinant proteins in mammalian cells. More in particular, the invention provides a cell for the production of a recombinant protein of interest wherein said cell is permissive to a polyomavirus and wherein said cell comprises the genetic elements A and B wherein A encodes a polyomaviral large T antigen or a functional equivalent thereof and B comprises a gene encoding a protein of interest under the functional control of a polyomaviral origin of replication or a functional equivalent thereof, wherein said cell lacks the capability to express a polyomaviral small T antigen or a functional equivalent thereof as well as the capability to express a polyomavirus capsid protein.Type: ApplicationFiled: October 26, 2010Publication date: September 20, 2012Applicant: AMARNA HOLDING B.V.Inventor: Walter Gerhardus de Vries
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Publication number: 20120238001Abstract: The present invention relates to the development and manufacturing of viral vaccines. In particular, the invention relates to the field of industrial production of viral vectors and vaccines, more in particular to the use of avian embryonic stem cells, preferably the EBx® cell line derived from chicken embryonic stem cells, for the production of viral vectors and viruses. The invention is particularly useful for the industrial production of viral vaccines to prevent viral infection of humans and animals.Type: ApplicationFiled: March 17, 2012Publication date: September 20, 2012Applicant: VIVALISInventors: Majid Mehtali, Patrick Champion-Arnaud, Arnaud Leon
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Publication number: 20120232133Abstract: Disclosed herein are compositions, systems and methods for delivery of proteins of interest using adeno-associated virus (AAV) vectors.Type: ApplicationFiled: February 21, 2012Publication date: September 13, 2012Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Alejandro Benjamin Balazs, David Baltimore
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Publication number: 20120231526Abstract: Methods of production and purification for viruses and virus-derived vectors, including those related to alphaviruses, are disclosed. In one aspect, methods of purification that subject alphavirus replicon particle preparations to one or more steps of chromatographic purification, such as using an ion exchange resin, are provided.Type: ApplicationFiled: March 27, 2012Publication date: September 13, 2012Inventors: John M. Polo, Catherine Greer, Maria Calderon-Cacia, Daniel De La Vega, JR., Thomas W. Dubensky, JR.
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Patent number: 8263395Abstract: The invention concerns compositions and methods for preparing recombinant adenoviruses. The resulting adenoviruses can be used for transferring and/or expressing genes in cells, in vitro, ex vivo or in vivo, or also in functional genomics. More particularly, the invention concerns in particular efficient methods for producing adenovirus banks and the use of said banks in functional genomics. The invention also concerns plasmids used for constructing said adenoviruses.Type: GrantFiled: December 8, 2008Date of Patent: September 11, 2012Assignee: Aventis Pharma S.A.Inventor: Jean-Jacques Robert
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Publication number: 20120219588Abstract: The present invention provides methods for the purification of cell-associated viruses from adherent cells (e.g., MDCK or Vero cells). In particular, the present invention provides purification methods for the production of immunogenic compositions comprising a live attenuated cell-associated virus (e.g., an attenuated respiratory syncytial virus (RSV) or cold-adapted, and/or temperature sensitive influenza virus) that result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.Type: ApplicationFiled: September 24, 2009Publication date: August 30, 2012Inventors: Mark Thompson, Janice Wee, Akanksha Nagpal
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Publication number: 20120213741Abstract: The present invention describes an efficient commercial scale production method for the preparation of PRRS virus.Type: ApplicationFiled: February 14, 2012Publication date: August 23, 2012Applicant: BOEHRINGER INGELHEIM VETMEDICA GMBHInventors: Elizabeth Jane BERRY, Fuad Tawfiq HADDADIN, Ali KHAZRAEINAZMPOUR, Jeremy KROLL, Sonia Regina Cantisano MALBURG, Edgar Arnulfo SANDOVAL BASURTO, Stephen SCHEERER
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Patent number: 8247207Abstract: Methods and compositions for the optimization and production of refrigerator-temperature stable virus, e.g., influenza, compositions are provided. Formulations and immunogenic compositions comprising refrigerator-temperature stable virus compositions are provided.Type: GrantFiled: June 18, 2009Date of Patent: August 21, 2012Assignee: Medimmune, LLCInventors: George Kemble, George Trager, Richard Schwartz
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Publication number: 20120202268Abstract: The invention provides methods for large-scale adenovirus purification from high cell density suspensions, using host cell DNA fragmentation and/or precipitation followed by a clarification step with tangential flow filtration.Type: ApplicationFiled: October 14, 2010Publication date: August 9, 2012Applicant: Crucell Holland B.V.Inventors: Marcel Leo De Vocht, Marloes Veenstra
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Publication number: 20120202267Abstract: The invention provides methods for large-scale adenovirus purification from high cell density suspensions, using host cell DNA precipitation followed by a clarification step.Type: ApplicationFiled: October 14, 2010Publication date: August 9, 2012Inventors: Marcel Leo De Vocht, Marloes Veenstra
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Publication number: 20120201852Abstract: The invention provides attenuated Flavivirus vaccines, such as vaccines against Japanese encephalitis virus and West Nile virus, as well as methods of making and using these vaccines.Type: ApplicationFiled: October 3, 2011Publication date: August 9, 2012Applicant: Sanofi Pasteur Biologics Co.Inventors: Farshad Guirakhoo, J. Jian Liu, John A. Catalan, Thomas P. Monath, Konstantin V. Pugachev
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Patent number: 8236560Abstract: The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime/vaccinia virus boost inoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera.Type: GrantFiled: May 12, 2011Date of Patent: August 7, 2012Assignee: Bavarian Nordic A/SInventors: Paul Chaplin, Paul Howley, Christine Meisinger-Henschel, Ingmar Rathe, Eva Felder, Karl Heller
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Publication number: 20120189656Abstract: Expression of a transgene is driven in a host cell using a pol I promoter which is not endogenous to an organism from the same taxonomic order from which the host cell is derived.Type: ApplicationFiled: May 21, 2010Publication date: July 26, 2012Inventors: Philip Dormitzer, Michael Franti, Peter Mason, Pirada Suphaphiphat, Bjoern Keiner, Stefania Crotta
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Publication number: 20120190100Abstract: The present invention relates to an enzymatic composition for the digestion of chicken embryos intended to the preparation of cells which are used for the production of viruses. The present invention also relates to a method for producing a wild type, an attenuated and/or a recombinant virus comprising a step of preparation of cells from chicken embryos using an enzymatic composition of the invention. The present invention relates to a purified wild type, attenuated and/or recombinant virus obtained and to a pharmaceutical composition, preferably a vaccine, comprising said virus for the treatment and/or the prevention a cancer, an infectious disease and/or an autoimmune disorder, and uses thereof.Type: ApplicationFiled: July 21, 2010Publication date: July 26, 2012Applicant: Transgene ASInventors: Martine Marigliano, Jacqueline Reymund, Martine Sainte-Marie
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Publication number: 20120190068Abstract: Disclosed is a method of increasing product yield per culture in a population of bound product-secreting cells in a bioreactor, the method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration; re-feeding the bound population of product-secreting cells by adding an amount of a fresh culture medium sufficient to increase the volume of the culture medium to approximately the original volume of the culture medium; agitating the culture medium under sufficient conditions and for a sufficient time period to allow the bound population of product-secreting cells to grow and to release a second-secreted product concentration into the culture medium; and harvesting product.Type: ApplicationFiled: January 20, 2012Publication date: July 26, 2012Applicant: Xcellerex, Inc.Inventors: Patrick M. GUERTIN, Joseph D. Crowell
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Patent number: 8221971Abstract: Adenovirus serotypes differ in their natural tropism. The adenovirus serotypes 2, 4, 5 and 7 all have a natural affiliation towards lung epithelia and other respiratory tissues. In contrast, serotypes 40 and 41 have a natural affiliation towards the gastrointestinal tract. The serotypes described, differ in at least capsid proteins (penton-base, hexon), proteins responsible for cell binding (fiber protein), and proteins involved in adenovirus replication. This difference in tropism and capsid protein among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins.Type: GrantFiled: October 29, 2007Date of Patent: July 17, 2012Assignee: Crucell Holland B.V.Inventors: Abraham Bout, Menzo Jans Emco Havenga, Ronald Vogels
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Publication number: 20120177675Abstract: The present invention provides a chimaeric coronavirus S protein which is based on an S protein from a coronavirus strain with restricted tissue tropism, but which comprises at least part of the S2 subunit from a coronavirus strain with extended tissue tropism, such that a virus comprising the chimaeric S protein has extended tissue tropism. The present invention also provides a virus comprising such a chimaeric S protein.Type: ApplicationFiled: July 5, 2010Publication date: July 12, 2012Applicant: INSTITUTE FOR ANIMAL HEALTHInventors: Paul Britton, Erica Bickerton, Maria Armesto
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Publication number: 20120171660Abstract: The present invention relates to methods for screening or purifying enteroviruses, a method for mass-producing enteroviruses, and a method for manufacturing an enterovirus vaccine. The method for screening enteroviruses in a sample comprises the following steps: (A) providing a sample and a carrier, wherein monosaccharides such as glucose or galactose are bound to the surface of the carrier, and the monosaccharides have binding affinity to enterovirus; (B) contacting the sample with the carrier; (C) removing components of the sample that do not bind to the carrier; (D) providing a detection unit and contacting the detection unit with the carrier, wherein the detection unit binds to the sample bound on the carrier; and (E) measuring a signal of the detection unit, wherein when the signal of the detection unit is detected, it represents that the enterovirus exists in the sample.Type: ApplicationFiled: July 14, 2011Publication date: July 5, 2012Applicant: National Cheng Kung UniversityInventors: Huan-Yao LEI, Chia-Ming Liu
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Publication number: 20120171750Abstract: The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.Type: ApplicationFiled: March 15, 2012Publication date: July 5, 2012Inventors: Sara Post Hansen, Rene Faber, Udo Reichl, Michael Wolff, Anders Peter Gram
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Patent number: 8211686Abstract: The present invention relates to methods for purification of Vaccinia viruses (W) and/or Vaccinia virus (W) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.Type: GrantFiled: August 23, 2011Date of Patent: July 3, 2012Assignee: Bavarian Nordic A/SInventors: Rene Djurup, Sara Post Hansen
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Publication number: 20120164710Abstract: The invention provides methods for the preparation of an isolated virus particle or virus-like particle by treating with an agent such that the particles are preferentially in the aqueous phase. The invention also provides methods of preparing a capsomere that is substantially-free of at least one host cell derived chaperone protein by treatment with an agent to selectively separate the capsomere from at least one chaperone protein.Type: ApplicationFiled: July 5, 2010Publication date: June 28, 2012Inventors: Yuan Yuan Fan, Anton PJ Middelberg