Abstract: An alkaline protease mutant, and a gene, engineered strain, a preparation method and application thereof are provided. The method comprises the following steps of extracting genome DNA of Bacillus clausii, performing PCR amplification to obtain a wild-type alkaline protease gene sequence, mutating the wild-type alkaline protease gene obtained by the amplification through an error-prone PCR, performing high-throughput screening to obtain a plurality of highly active alkaline protease genes, performing DNA shuffling on the highly active alkaline protease genes, and performing screening to obtain eight alkaline protease mutant genes with higher activity.

Abstract: The present disclosure relates to detergent compositions comprising subtilase variants exhibiting improved stability and/or improved wash performance in liquid detergent and methods for obtaining such detergent compositions. The variants are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.

Abstract: An aqueous disinfecting solution and uses and methods of disinfection and sanitization employing same. The solution comprises at least one inorganic salt selected from the group consisting of sulfate, chloride, bromide, iodide, carbonate, phosphate, fluoride, and nitrate salts in a concentration of from about 0.1 to about 15 wt. %; at least one peroxide compound in a concentration of from about 0.01 to about 8 wt. %; and at least one amphoteric amine oxide surfactant having the formula R1R2R3NO wherein each of R1, R2, and R3 is independently a saturated, unsaturated, substituted or unsubstituted linear or branched alkyl group having from 1 to 28 carbon atoms in a concentration of from about 0.01 to about 15 wt. %.

Abstract: Orally administered physiologically acceptable anti-biofilm compositions comprising enzymes and if desired additional components such as antimicrobials, antibiotics, antifungals, herbals, chelating agents, lactoferrin and related compounds, minerals, surfactants, binders, and fillers useful for the inhibition and treatment of gastrointestinal biofilms in humans. Physiologically acceptable anti-biofilm compositions containing these enzymes are useful in the inhibition, reduction and/or treatment of gastrointestinal biofilm infections, and associated systemic symptoms caused by biofilms associated microorganisms within the gastrointestinal tract. Methods of identification, preparation and use of such physiologically acceptable anti-biofilm compositions are also provided.

Abstract: The present invention concerns the use of a polypeptide having deoxyribonuclease (DNase) activity for preventing, reducing or removing static electricity from an item; a composition comprising such polypeptide; a method for reducing or removing static electricity from an item; and a wipe for such use.

Abstract: Oxygenases and methods of biologically upgrading hydrocarbon streams, such as crude oil, using oxygenases are provided herein. The oxygenases can be used to remove impurities such as metals, heteroatoms, or asphaltenes from a hydrocarbon stream. In some cases, the oxygenases can be chemically or genetically modified and can be used in different locations such as petroleum wells, pipes, reservoirs, tanks and/or reactors.

Abstract: The present invention relates to methods of increasing the activity of a polypeptide having cellulolytic enhancing activity, comprising: adding a soluble activating divalent metal cation to a composition comprising the polypeptide having cellulolytic enhancing activity, wherein the presence of the soluble activating divalent metal cation and the polypeptide having cellulolytic enhancing activity increases degradation or conversion of a cellulose-containing material by a cellulolytic enzyme composition compared to the polypeptide having cellulolytic enhancing activity without the soluble activating divalent metal cation. The present invention also relates to compositions, methods for degrading or converting a cellulose-containing material, and methods for producing a fermentation product.

Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.

Abstract: A microbial dressing method for super abrasive tools includes a kind of microbe which is capable of consuming the bond in a certain manner is selected to perform the microbial dressing. Specifically, the microbe is inoculated and cultured in the culture medium to a certain concentration, then the dressing area of the abrasive tool is immersed into the culture liquid to remove the bond in the surface by the action of the microbe, and the control of the dressing amount is realized by controlling the microbe concentration and the soaking time. Precise dressing for super abrasive products, particularly for fine grained abrasive tools can be realized using the present method.

Abstract: Methods and apparatuses for inactivating RNA and DNA within viruses situated on medical instruments. Through the use of photochemicals and photoactivation by monochromatic light, medical instruments can be rendered safer. A solution of methylene blue having a concentration of 0.01-0.02% by means of interaction with light in the emission spectrum of monochromatic emitters having wavelengths ranging between 582 and 592 nm or between 658 and 662 nm and an overall light output of at least 280 lumens (lm), wherein the instruments are kept in said solution for about 90 minutes.

Abstract: Solid compositions for use in textile treatment, each comprising, based on the total weight of the solid composition, a total amount of each of —30% to 70% by weight of at least one peroxide compound, —10% to 20% by weight of at least one organic bleach activator, —5% to 50% by weight of hydrogencarbonate, calculated as sodium hydrogencarbonate, —0% to 5% by weight of surfactant, where the total amount of organic compound in the solid composition is 10% to 50% by weight, preferably 20% to 35% by weight, are thermally stable and can be stored reliably. They are suitable as an additive to conventional textile treatment compositions, especially to liquid washing compositions, and can be provided as a portion encased with a water-soluble material.

Abstract: The invention concerns a method for prophylaxis of infections by fungi, particularly by oomycetes, and of bacterial infections in crop and ornamental plants. Areas of application are vine, fruit, vegetable and ornamental plant growing. The method of the invention is characterized in that an aqueous solution of a protease, alone or in combination with ß-glucanases and/or chitinases, is prepared, stabilizers, stickers and wetters are added, and the additized solution is applied by conventional techniques to the plants a number of times within the vegetation period, preferably ahead of weather-related phases of high infection threat.

Abstract: The present invention relates to apparatus, methods, and applications for treating wastewater, and more particularly to biological processes for removing pollutants from wastewater. This invention further relates to apparatus and methods for growing microbes on-site at a wastewater treatment facility, and for economically inoculating sufficient microbes to solve various treatment problems rapidly.

Abstract: The invention is directed toward non-wild-type organophosphorus acid anhydrolases having three site mutations, method of production, and method of use to effectively degrade toxic chemical compounds such as (Ethyl({2-[bis(propan-2-yl)amino]ethyl}sulfanyl)(methyl)phosphinate (“VX”).

Abstract: The invention is directed toward non-wild-type organophosphorus acid anhydrolases having three site mutations, method of production, and method of use to effectively degrade toxic chemicals such as N,N-diethyl-2-(methyl-(2-methylpropoxy)phosphoryl)sulfanylethanamine) (“VR”).

Abstract: The present invention relates to [1] a detergent composition for a washer for medical devices, including a nonionic surfactant (A) represented by the following formula (1), at least one compound (B) selected from the group consisting of fatty acids having not less than 6 and not more than 10 carbon atoms or salts thereof, a cationic surfactant (C) represented by the specific formula, and water; and [2] a method of washing a medical device using a washer for medical devices which includes the step of washing the medical device with a mixture including the above detergent composition and an enzyme.

Abstract: The invention relates to a cleaning composition comprising a lipase with at least 75% identity to SEQ ID NO: 2, and a surfactant, wherein said composition is more effective in removing lipid stains present at a surface in comparison with an equivalent composition lacking the lipase.

Abstract: Stabilized use solutions of low phosphorus, alkali metal carbonate detergents employing enzymes for cleaning compositions are disclosed. In particular, the present invention is a composition for, and method of, removing soils, preventing redeposition of protein soils and reducing foam, using stabilized enzyme cleaning compositions, namely use solutions of the same.

Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.

Abstract: Stabilized use solutions of low phosphorus, alkali metal carbonate detergents employing enzymes for cleaning compositions are disclosed. In particular, the present invention is a composition for, and method of, removing soils, preventing redeposition of protein soils and reducing foam, using stabilized enzyme cleaning compositions, namely use solutions of the same.

Abstract: The present invention relates to isolated polypeptides having xanthan degrading activity, catalytic domains and polynucleotides encoding the polypeptides and catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and catalytic domains.

Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.

Abstract: The present invention generally relates to compositions and methods of delivering substances in a dry mode, wherein the compositions include a surface layer disposed on the outer surface of the composition that is permeable to carbon dioxide and oxygen. The compositions may be used to deliver microorganisms to remove contaminates, such as oil, chemical, waste, or sewage, from soil, water, or air. In other embodiments, the compositions can also be used for delivering liquid food, liquid food additives, liquid biotech agricultural ingredients, conventional liquid agricultural ingredients, liquid human wellness and dietary supplements, and liquid fragrances and beauty products.

Abstract: The present invention provides consumer products comprising a serine protease variant, more specifically subtilisin variants produced there from. Specifically, the present invention provides consumer products comprising a serine protease variant, more specifically a subtilisin variant having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides methods of making such compositions and methods of treating surfaces, particularly fabric surfaces comprising contacting a surface with an aqueous liquor comprising a serine protease variant, more specifically subtilisin variants and an adjunct material.

Abstract: The objective of the present invention is in the field of cleaning agent in particular detergents. In particular, it relates to a novel detergent formulation for an automatic dishwashing. The formulation provides excellent cleaning and finishing; it is environmentally friendlier than traditional compositions and allows for a more energy efficient automatic dishwashing process.

Abstract: The object of the present invention is a composition of bacterial strains which may comprise Stenotrophomonas sp. strain 2L, Stenotrophomonas sp. strain 5L, Stenotrophomonas sp. strain 6L, Stenotrophomonas sp. strain 3N, Achromobacter sp. strain 4P, Arthrobacter sp. strain 1N, Brevundimonas sp. strain 2N, Brevundimonas sp. strain 5N, Brevundimonas sp. strain 6N, Pseudomonas sp. strain 3G, and Pseudomonas sp. strain 4, deposited under the number KKP 2041p (IAFB Collection of Industrial Microorganisms—Institute of Agricultural and Food Biotechnology in Warsaw), a bioremediation vaccine (bioremediation mixture) which may comprise the composition of these strains, the use of the vaccine in the removal of contaminants from the soil, and the method for the treatment of contaminated soil.

Abstract: A detergent composition comprising a polypeptide having protease activity is disclosed. The composition may be used in laundry or hard surface cleaning such as automated dish wash, and it has improved performance compared with a commercial protease.

Abstract: The present invention relates to a method of selectively obtaining phosphorus from solids containing heavy metals and phosphate. In this method, the solid is treated under acidic aerobic conditions using microorganisms comprising leaching microorganisms and polyphosphate-accumulating microorganisms, so that the heavy metals and the phosphates are released from the solid and the released phosphates may be taken up by the polyphosphate-accumulating microorganisms. The phosphorus-enriched biomass that is obtained in this manner is separated and may be utilized, e.g., as organic fertilizer.

Abstract: The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.

Abstract: The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant proteases suitable for laundry cleaning applications.

Abstract: A method for degumming an oil composition comprises the steps of (a) providing an oil composition containing a quantity of phospholipids, (b) contacting said oil composition simultaneously with one or more phospholipase A enzymes and one or more phospholipase C enzymes, under conditions sufficient for the enzymes to react with the phospholipids to create phospholipid reaction products, and (c) separating the phospholipids reaction products from the oil composition, the remaining oil composition after the separation being a degummed oil composition, whereby during step (b) the reaction of said one or more phospholipase A enzymes proceeds at a faster rate than it would in the absence of said one or more phospholipase C enzymes.

Abstract: Described are compositions and methods relating to a fungal cutinase cloned from Magnaporthe grisea, polynucleotides encoding the cutinase, and methods of use thereof. The compositions and methods have particular application in detergent cleaning compositions and synthesis reactions.

Abstract: The invention relates to the use of a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having ?-1,3-endoglucanase activity in a process of lysing yeast cell walls as well as to a process for lysing yeast cell walls which comprises exposing said yeast cell walls to a composition comprising at least one polypeptide having glucoamylase activity and optionally at least one polypeptide having ?-1,3-endoglucanase activity.

Abstract: The present invention relates to isolated lipase variants, comprising a substitution at one or more positions corresponding to positions I86D,E,N,Q, E87C, I90D,E,Q, and N92D,E,Q of the mature polypeptide of SEQ ID NO: 2, wherein the variant has lipase activity. In some embodiments the present invention relates to isolated polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; methods of producing the variants; and compositions comprising the variants. The present invention also relates to methods of obtaining lipase variants; methods of cleaning; and use of lipase variants for cleaning.

Abstract: The present invention relates to detergent compositions comprising subtilisin variants and methods for obtaining such detergent compositions. The present invention also relates to the use of such detergent compositions, especially in laundry or in hard surface cleaning applications.

Abstract: The present invention addresses the problem of providing a new microorganism that is useful for efficiently decomposing oils and fats, and a use for said microorganism. According to screening results, a new Yarrowia lipolytica having a high capacity to assimilate free fatty acids was successfully obtained. Efficient decomposition of oils and fats is achieved by causing the Yarrowia lipolytica to act under conditions in which fatty acids that are hydrolysis products of oils or fats are present, or under conditions in which oils or fats are decomposed into fatty acids and glycerol.

Abstract: Exemplary embodiments provide methods and apparatus of using an enzyme-surfactant solution to control surface wettability of a xerographic print.

Abstract: In a liquid dishwashing detergent comprising a protease and amylase, storage stability is to be improved. This is achieved through the use of a protease comprising an amino acid sequence having at least 70% identity over its total length with the amino acid sequence specified in SEQ ID NO. 1 and having, in the listing according to SEQ ID NO. 1, the L211D amino acid substitution in combination with at least two further amino acid substitutions selected from the group consisting of S3T, V4I, V193M and V199I.

Abstract: The present invention provides lipolytic enzyme variants. Specifically, the present invention provides lipolytic enzyme variants having two, three, or more modifications as compared to a parent lipolytic enzyme having at least one improved property. In addition, the present invention provides compositions comprising a lipolytic enzyme variant of the invention. The present invention also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the invention.

Abstract: The present invention relates to a method for the degradation of pollutants in water and/or soil. More specific, the present invention relates to a method for the on-site decontamination or re-mediation of water and/or soil which are contaminated with organic compounds. Moreover, the invention relates to a method for forming a barrier against the spreading of a contamination with pollutants within the water and/or soil, especially within groundwater (aquifer). Further, the invention relates to means for use in these methods, and to the production of such means.

Abstract: Temporary active coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Abstract: The present invention relates to microbial compositions useful in treating and remediating wastewater, removing organic matter from the surfaces of post harvested fruits and vegetables, and decreasing post-harvest disease in fruit and vegetables.

Abstract: Methods and products for spill response management also providing bioremediation of specific substrates such as protein, starch, carbohydrates, hydrocarbons, fats and greases through superior enzymatic activity in both aerobic and anaerobic environments with one product. In one illustrative embodiment, a product may include coir in a proportion of from about 85% to about 99% and a suitable microbe for initiating bioremediation in an appropriate form in a proportion of from about 20% to about 1%, by weight. Methods of using a product may include absorbing spilled fluid with the product then disposing of the product with the absorbed fluid where the microbe will perform bioremediation. This may allow the absorption (cleanup) of commonly used hazardous and non-hazardous chemicals in residential, commercial and industrial applications and the peace of mind of using a safe, sustainable, environmentally friendly, landfill safe product.

Abstract: Compositions and methods relating to variant alpha-amylases are described.

Abstract: A hydrolytic enzyme is stabilized in a liquid surfactant preparation using a component that stabilizes the hydrolytic enzyme and includes a phenylalkyldicarboxylic acid.

Abstract: Described are compositions and methods for the decolorization of dye molecules in an aqueous medium using enzymatically-generated peracid.

Abstract: A hydrolytic enzyme is stabilized in a liquid surfactant preparation using a component that stabilizes the hydrolytic enzyme and encompasses an oligoaminobiphenyl oligocarboxylic acid.

Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations.

Abstract: Composition for the removal of biofilms present on a substrate, comprising at least one detergent component comprising at least one sequestering agent and at least one agent that is simultaneously a wetting and a dispersing agent, and at least one enzymatic component containing at least one protease, at least one laccase and at least one polysaccharidase

Abstract: A genetically modified bacteriophage is disclosed which comprises: (i) an exogenous polynucleotide which encodes an agent which reduces the toxicity of a bacterium; and (ii) an exogenous polynucleotide which encodes a selectable marker. Uses thereof and kits comprising same are also disclosed.