Vector, Per Se (e.g., Plasmid, Hybrid Plasmid, Cosmid, Viral Vector, Bacteriophage Vector, Etc.) Bacteriophage Vector, Etc.) Patents (Class 435/320.1)
  • Patent number: 10662440
    Abstract: Disclosed herein are viral vectors for use in recombinant molecular biology techniques. In particular, the present disclosure relates to self-limiting viral vectors comprising genes encoding site-specific endonucleases as well as recognition sequences for site-specific endonucleases such that expression of the endonuclease in a cell cleaves the viral vector and limits its persistence time. In some embodiments, the viral vectors disclosed herein also carry directives to delete, insert, or change a target sequence.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: May 26, 2020
    Assignee: Precision BioSciences, Inc.
    Inventors: Derek Jantz, James Jefferson Smith, Michael G. Nicholson
  • Patent number: 10660950
    Abstract: Described herein are processes for purifying infectious virus particles and uses of protamine in such processes.
    Type: Grant
    Filed: December 23, 2016
    Date of Patent: May 26, 2020
    Assignee: Valneva SE
    Inventors: Jana Barbero Calzado, Mario Nebenführ, Robert Schlegl, Michael Weber, Jürgen Heindl-Wruss
  • Patent number: 10655144
    Abstract: This disclosure provides non-human animal models for age-related disorders and age-sensitive traits, particularly those caused by senescence-inducing stimuli, wherein the models comprise transgenes selectively expressed by senescent cells. The disclosure further provides methods for identifying therapeutic agents effective for treating or preventing age-related disorders and age-sensitive traits using the animal models, therapeutic agents identified using such methods, pharmaceutical compositions comprising the identified therapeutic agents, and methods of treating or preventing age-related disorders and age-sensitive traits.
    Type: Grant
    Filed: January 12, 2018
    Date of Patent: May 19, 2020
    Assignee: Buck Institute for Research on Aging
    Inventors: Marco Demaria, Francis Rodier, Remi-Martin Laberge, Judith Campisi
  • Patent number: 10647751
    Abstract: An adeno-associated viral (AAV) vector containing an expression construct, wherein: the expression construct comprises a nucleic acid sequence which encodes a microdystrophin (MD); and the nucleic acid sequence encoding the MD has a size of at least 4.1 kb.
    Type: Grant
    Filed: May 9, 2016
    Date of Patent: May 12, 2020
    Assignee: ROYAL HOLLOWAY & BEDFORD NEW COLLEGE
    Inventor: George Dickson
  • Patent number: 10647991
    Abstract: The present invention provides for compositions and methods for producing sorghum crop plants that are resistant to herbicides. In particular, the present invention provides for sorghum plants, plant tissues and plant seeds that contain altered acetolactate synthase (ALS) genes and proteins that are resistant to inhibition by herbicides that normally inhibit the activity of the ALS protein.
    Type: Grant
    Filed: January 26, 2017
    Date of Patent: May 12, 2020
    Assignee: KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
    Inventors: Mitchell R. Tuinstra, Kassim Al-Khatib
  • Patent number: 10647965
    Abstract: The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: May 12, 2020
    Assignees: BASF SE, THE UNIVERSITY COURT OF THE UNIVERSITY OF EDINBURGH
    Inventors: Frank Meulewaeter, Ilse Van Den Brande, Stephen C. Fry, Kyle E. Mohler, Lenka Frankova, Tom J. Simmons, Claire Holland, Andrew Hudson
  • Patent number: 10640762
    Abstract: Methods of making and using bacterial display polypeptide libraries using circularly permuted OmpX (CPX) variants are disclosed. The invention further relates to methods for enhancing the display of proteins and peptides at the surface of bacteria by optimizing linkers and incorporating mutations at positions 165 and 166 of CPX.
    Type: Grant
    Filed: June 5, 2017
    Date of Patent: May 5, 2020
    Assignee: The Regents of the University of California
    Inventors: Patrick Sean Daugherty, Jeffrey Rice
  • Patent number: 10631487
    Abstract: A soybean cultivar designated 83240828 is disclosed. The invention relates to the seeds of soybean cultivar 83240828, to the plants of soybean cultivar 83240828, to the plant parts of soybean cultivar 83240828, and to methods for producing progeny of soybean cultivar 83240828. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 83240828. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 83240828, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 83240828 with another soybean cultivar.
    Type: Grant
    Filed: June 6, 2019
    Date of Patent: April 28, 2020
    Assignees: Monsanto Technology LLC, Stine Seed Farm, Inc.
    Inventor: William H. Eby
  • Patent number: 10626406
    Abstract: The invention relates to the field of plant improvement, in particular of the improvement of yield for plants, by using a transgene containing an root-specific promoter driving expression of a MYB-related protein.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: April 21, 2020
    Assignee: GENOPLANTE-VALOR
    Inventors: François Torney, Jérôme Martin, Stéphane Lafarge
  • Patent number: 10626378
    Abstract: In certain embodiments, the disclosure relates to vectors containing bacterial nucleic acid sequences and a paramyxovirus gene. Typically, the expression vector comprises a bacterial artificial chromosome (BAC), and a nucleic acid sequence comprising a respiratory syncytial virus (RSV) gene in operable combination with a regulatory element and optionally a reporter gene.
    Type: Grant
    Filed: February 14, 2019
    Date of Patent: April 21, 2020
    Assignees: Emory University, Children's Healthcare of Atlanta, Inc.
    Inventors: Martin L. Moore, Anne Hotard
  • Patent number: 10626471
    Abstract: This invention is related to the area of characterization of inflammation in relation with the gut microbiota, in metabolic and autoimmune disorders. In particular, it relates to the identification of gene signatures which can be used as a marker predictive of inflammation associated diseases, such as liver-related metabolic disorders, in particular to the evolution of benign steatosis towards its most severe forms (steatohepatitis and cirrhosis) or autoimmune disorders, in particular inflammatory bowel diseases (Crohn's and Ulcerative Colitis). These gene signatures can therefore be used as a means of diagnosis, prognosis, stratification for drug studies, for monitoring patient and for assigning an appropriate treatment.
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: April 21, 2020
    Assignees: ENTEROME, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
    Inventors: Matthieu Pichaud, Pierre Rimbaud, Stanislav Ehrlich
  • Patent number: 10617755
    Abstract: This invention concerns methods of treating a patient diagnosed with glioblastoma comprising administering to said patient a therapy comprising an effective amount of an anti-VEGF antibody and a chemotherapeutic.
    Type: Grant
    Filed: August 27, 2014
    Date of Patent: April 14, 2020
    Assignee: Genentech, Inc.
    Inventors: Carlos Bais, Richard Bourgon, Heidi Phillips, Thomas Sandmann
  • Patent number: 10619146
    Abstract: The present invention is directed to non-cytotoxic protein conjugates for inhibition or reduction of exocytic fusion in a nociceptive sensory afferent cell. The protein conjugates comprise: (i) a Targeting Moiety (TM), wherein the TM is an agonist of a receptor present on a nociceptive sensory afferent cell, and wherein the receptor undergoes endocytosis to be incorporated into an endosome within the nociceptive sensory afferent cell; (ii) a non-cytotoxic protease or a fragment thereof, wherein the protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of the nociceptive sensory afferent cell; and (iii) a Translocation Domain, wherein the Translocation Domain translocates the protease or protease fragment from within the endosome, across the endosomal membrane, and into the cytosol of the nociceptive sensory afferent cell wherein the Targeting Moiety is selected from the group consisting of BAM, ?-endorphin, bradykinin, substance P, dynorphin and/or nociceptin.
    Type: Grant
    Filed: September 7, 2016
    Date of Patent: April 14, 2020
    Assignees: Ipsen Bioinnovation Limited, Allergan Inc.
    Inventors: Keith Foster, John Chaddock, Charles Penn, Kei Roger Aoki, Joseph Francis, Lance Steward
  • Patent number: 10618946
    Abstract: The present invention pertains to an N-terminally truncated interleukin (IL)-38 protein, or functional variants thereof, as well as to nucleic acids and vectors encoding the truncated IL-38 peptide and recombinant cells comprising these nucleic acids or vectors. The invention shows that IL-38 is N-terminally processed and that the truncated version of the cytokine acts as an antagonist of immune activation in macrophages. This indicates a use of the truncated cytokine in the treatment and prevention of autoimmune disorders. The invention further provides pharmaceutical compositions comprising the truncated IL-38 protein, and method for screening modulators of the function of truncated IL-38.
    Type: Grant
    Filed: July 14, 2015
    Date of Patent: April 14, 2020
    Assignee: FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
    Inventors: Andreas Weigert, Javier Mora, Bernhard Brune, Christina Dillmann, Michael John Parnham, Gerd Geisslinger
  • Patent number: 10619163
    Abstract: Provided in certain aspects are methods for dynamically adding molecular indexes to nucleic acid, and optionally analyzing the tagged nucleic acid. Also provided in certain aspects are methods for producing a single-stranded nucleic acid molecule from two molecules. The first molecule typically is a single-stranded nucleic acid (ssNA) containing a target sequence with optional linked nucleic acid sequences. The second molecule typically is a ssNA containing a target binding sequence and a nucleic acid sequence “tag” that is not complementary to the target sequence.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: April 14, 2020
    Assignee: RHODX, Inc.
    Inventor: Charles Rodi
  • Patent number: 10612036
    Abstract: Engineered and modified Cry6Aa insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
    Type: Grant
    Filed: August 10, 2016
    Date of Patent: April 7, 2020
    Assignee: DOW AGROSCIENCES LLC
    Inventors: Timothy D. Hey, Xu Xiaoping, Todd P. Glancy, Diaa Alabed, Sarah E. Worden, Nick X. Wang, Carla Ausmus, Shao-Ching Hung
  • Patent number: 10602710
    Abstract: A soybean cultivar designated 89282206 is disclosed. The invention relates to the seeds of soybean cultivar 89282206, to the plants of soybean cultivar 89282206, to the plant parts of soybean cultivar 89282206, and to methods for producing progeny of soybean cultivar 89282206. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 89282206. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 89282206, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 89282206 with another soybean cultivar.
    Type: Grant
    Filed: June 7, 2019
    Date of Patent: March 31, 2020
    Assignees: Monsanto Technology LLC, Stine Seed Farm, Inc.
    Inventor: William H. Eby
  • Patent number: 10602701
    Abstract: The present invention provides an inbred corn line designated KIJ6559, methods for producing a corn plant by crossing plants of the inbred line KIJ6559 with plants of another corn plant. The invention further encompasses all parts of inbred corn line KIJ6559, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line KIJ6559, and plants produced according to these methods.
    Type: Grant
    Filed: November 8, 2018
    Date of Patent: March 31, 2020
    Assignee: Syngenta Crop Protection AG
    Inventor: Thomas Joseph Prest
  • Patent number: 10597674
    Abstract: In the present invention, HPPD polypeptides and plants containing them showing a full tolerance against one or more HPPD inhibitor herbicides belonging to various chemical classes are described. A set of mutant HPPD polypeptides have been designed which have either no or only a significantly reduced affinity to HPPD inhibitor herbicides and, at the same time, the rate of dissociation of the HPPD inhibitors of the mutant HPPD polypeptide is increased to such an extent that the HPPD inhibitors no longer act as slow-binding or slow, tight-binding inhibitors but, instead of this, have become fully reversible inhibitors. In particular, isolated polynucleotides encoding mutant HPPD polypeptides conferring tolerance to HPPD inhibitor herbicides belonging to various chemical classes are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: March 24, 2020
    Assignee: BASF Agricultural Solutions Seed, US LLC
    Inventors: Marc Linka, Fabien Poree, Bernd Laber, Gudrun Lange, Jan Tebbe, Wayne Coco, Michael Strerath, Ernst Weber, Nikolaus Pawlowski, Sandra Geske, Heike Balven-Ross, Nina Wobst, Christina Thies, Manuel Dubald
  • Patent number: 10597725
    Abstract: The present disclosure provides a novel primer reagent for detecting a plurality of variants of the ALK fusion gene.
    Type: Grant
    Filed: September 1, 2015
    Date of Patent: March 24, 2020
    Assignee: ARKRAY, Inc.
    Inventor: Marifu Yamagishi
  • Patent number: 10597669
    Abstract: The invention provides methods for engineering plants to have reduced levels of acetylation by decreasing expression of one or more Cas1L genes. Such plants can be used, e.g., to increase yield for biofuel production.
    Type: Grant
    Filed: July 18, 2016
    Date of Patent: March 24, 2020
    Assignee: The Regents of the University of California
    Inventor: Henrik Vibe Scheller
  • Patent number: 10590433
    Abstract: Provided herein are methods for identifying high potency genomic insulator elements that can be used in a vector composition e.g., that are useful for preventing unwanted expression of neighboring genes, such as proto-oncogenes, when administered to a subject in need thereof. Also provided herein are methods for treating disease and methods for administering a nucleic acid to a subject using such vectors.
    Type: Grant
    Filed: March 13, 2015
    Date of Patent: March 17, 2020
    Assignee: University of Washington
    Inventors: George Stamatoyannopoulos, John Stamatoyannopoulos
  • Patent number: 10583200
    Abstract: A method of targeting bacterially-derived, intact minicells to specific, non-phagocytic mammalian cells employs bispecific ligands to deliver nucleic acids efficiently to the mammalian cells. Bispecific ligands, comprising (i) a first arm that carries specificity for a bacterially-derived minicell surface structure and (ii) a second arm that carries specificity for a non-phagocytic mammalian cell surface receptor are useful for targeting minicells to specific, non-phagocytic mammalian cells and causing endocytosis of minicells by non-phagocytic cells.
    Type: Grant
    Filed: June 4, 2018
    Date of Patent: March 10, 2020
    Assignee: EnGeneIC Molecular Delivery Pty Ltd.
    Inventors: Himanshu Brahmbhatt, Jennifer MacDiarmid
  • Patent number: 10584346
    Abstract: The present invention relates to new methods to study and control the expression of plant genes, particularly nucleotide sequences located downstream from regions comprising binding sites for transcription factors, such as the cis-element translocon 1 (TL1) comprising GAAGAAGAA and similar sequences. The invention relates to isolated nucleotide sequences comprising a regulatory region comprising a promoter operably-linked to one or more upstream open reading frames (uORFs) and one or more downstream open reading frames (dORFs) encoding one or more functional polypeptides, including transcription factors such as TBF1, reporter polypeptides, and polypeptides conferring resistance to drugs, resistance of plants viral, bacterial, or fungal pathogens, and polypeptides involved in the growth of plants.
    Type: Grant
    Filed: June 8, 2018
    Date of Patent: March 10, 2020
    Assignee: Duke University
    Inventors: Karolina M. Pajerowska-Mukhtar, Guoyong Xu, Xinnian Dong
  • Patent number: 10576107
    Abstract: Methods for treating joint diseases, disorders, and conditions, such as osteoarthritis, with a composition containing a therapeutically effective amount of tissue inhibitors of matrix metalloproteinases (TIMPs) are provided. The composition can contain TIMPs as part of amniotic fluid, or the TIMPs can be isolated from another source or recombinantly produced. The methods are particularly useful for treating osteoarthritis associated with degradation of articular cartilage by proteases. Also provided are methods of lubricating a joint, such as an osteoarthritic joint.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: March 3, 2020
    Assignee: Prime Merger Sub, LLC
    Inventors: Jeffrey S. White, Robin R. Young
  • Patent number: 10571471
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Grant
    Filed: February 5, 2016
    Date of Patent: February 25, 2020
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • Patent number: 10571460
    Abstract: This disclosure provides methods, systems, and compositions of matter for studying solvent accessibility and three-dimensional structure of biological molecules. A plasma can be used to generate marker radicals, which can interact with a biological molecule and mark the solvent-accessible portions of the biological molecule.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: February 25, 2020
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Michael R. Sussman, J. Leon Shohet, Faraz A. Choudhury, Joshua M. Blatz, Benjamin B. Minkoff, Daniel I. Benjamin
  • Patent number: 10570398
    Abstract: Certain embodiments provide methods and compositions related to clinical management of cancer patients based on the expression level of TMCO3. Further embodiments involve methods and compositions related to treatment of cancer patients or patients determined to have an increased TMCO3 level relative to a control or a reference level that is normal or indicating favorable prognosis.
    Type: Grant
    Filed: March 17, 2016
    Date of Patent: February 25, 2020
    Assignee: Baylor Research Institute
    Inventors: Ajay Goel, Kunitoshi Shigeyasu
  • Patent number: 10570440
    Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: February 25, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventor: James White
  • Patent number: 10563220
    Abstract: The invention provides recombinant DNA molecules and constructs useful for providing efficient transgene sub-cellular localization of proteins in transgenic plants. Recombinant DNA molecules and constructs for conferring herbicide tolerance or resistance to plants are further provided, as well as plants exhibiting herbicide tolerance and methods for producing or utilizing such plants.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: February 18, 2020
    Assignee: Monsanto Technology LLC
    Inventors: Christine M. Ellis, Michael E. Goley, Clayton T. Larue, Sherry L. LeClere, Qungang Qi, Aihua Shao, Kwan Y. Thai
  • Patent number: 10562981
    Abstract: The disclosure provides a method for generation of humanized full length antibodies in mammalian cells. A library of humanized variants is provided with high, validated human framework diversity without requiring back-mutations to retain original affinity. Synthetic CDR encoding fragment libraries derived from a template antibody are ligated to human framework region encoding fragments from a human framework pool limited only to germline sequences from a functionally expressed antibodies. The vector comprises a nucleic acid sequence encoding HC framework region 4. No CDR grafting or phage display is required.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: February 18, 2020
    Assignee: BioAtla, LLC
    Inventor: Jay M. Short
  • Patent number: 10556949
    Abstract: The present inventors have discovered that in living organisms that have received an antigen-binding molecule containing an antigen-binding domain whose binding activity to an antigen changes depending on ion concentration conditions and containing an FcRn-binding domain having FcRn-binding activity in a neutral pH range, immune responses to the antigen are induced. Furthermore, the present inventors have discovered that in living organisms that have received an antigen-binding molecule containing an antigen-binding domain whose binding activity to an antigen changes depending on ion concentration conditions and containing an FcRn-binding domain having FcRn-binding activity in a neutral pH range, immune responses to the antigen are induced, and also the antigen-binding molecule has cytotoxicity or antiproliferative action against cancer cells, foreign biological species, or such that express the antigen to which the antigen-binding molecule binds.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: February 11, 2020
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventors: Tomoyuki Igawa, Atsuhiko Maeda, Kenta Haraya, Tatsuhiko Tachibana
  • Patent number: 10550406
    Abstract: Methods and compositions are provided for nuclease-mediated gene editing of primary cells without the use of viral mediated delivery. Methods of treatments using edited primary cells are also provided.
    Type: Grant
    Filed: August 27, 2019
    Date of Patent: February 4, 2020
    Assignee: PACT PHARMA, INC.
    Inventors: Kyle Jacoby, Alex Franzusoff
  • Patent number: 10548287
    Abstract: A soybean cultivar designated 86411138 is disclosed. The invention relates to the seeds of soybean cultivar 86411138, to the plants of soybean cultivar 86411138, to the plant parts of soybean cultivar 86411138, and to methods for producing progeny of soybean cultivar 86411138. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 86411138. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 86411138, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 86411138 with another soybean cultivar.
    Type: Grant
    Filed: June 5, 2019
    Date of Patent: February 4, 2020
    Assignees: Monsanto Technology LLC, Stine Seed Farm, Inc.
    Inventor: William H. Eby
  • Patent number: 10550415
    Abstract: The present invention relates to a system for production of ATP. This system is comprised of a support and one or more enzymes coupled to that support which are capable of collectively producing ATP from glucose or fructose metabolism. The present invention is additionally directed to a device, which includes the system, and to a method for carrying out a reaction involving the conversion of ATP to ADP using the system.
    Type: Grant
    Filed: October 16, 2008
    Date of Patent: February 4, 2020
    Assignee: Cornell Research Foundation, Inc.
    Inventor: Alexander Travis
  • Patent number: 10544466
    Abstract: A method increases permeability of a blood-brain barrier in a subject. The method includes administering a small interfering ribonucleic acid (siRNA) against the 3-Phosphoinositide Dependent Protein Kinase 1 (PDPK1) gene or micro ribonucleic acid (miRNA) miR-181c as an active ingredient to the subject. The method can be applied for delivering a desired active agent to the subject.
    Type: Grant
    Filed: February 24, 2015
    Date of Patent: January 28, 2020
    Assignees: Gene Techno Science Co., Ltd., National Cancer Center, Theoria Science Inc.
    Inventors: Takahiro Ochiya, Naoomi Tominaga
  • Patent number: 10543257
    Abstract: The invention is directed to a more efficient lentiviral vector comprising a nucleic acid sequence encoding a human ?-globin protein or a human ?-globin protein, which is oriented from 5? to 3? relative to the lentiviral genome. The invention also provides a composition and method utilizing the lentiviral vector.
    Type: Grant
    Filed: August 14, 2015
    Date of Patent: January 28, 2020
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Naoya Uchida, John F. Tisdale
  • Patent number: 10542707
    Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV653095. The invention thus relates to the plants, seeds and tissue cultures of the variety CV653095, and to methods for producing a corn plant produced by crossing a corn plant of variety CV653095 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV653095 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV653095.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: January 28, 2020
    Assignee: Monsanto Technology LLC
    Inventor: Jon Popi
  • Patent number: 10544401
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Grant
    Filed: February 11, 2019
    Date of Patent: January 28, 2020
    Assignee: Codexis, Inc.
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Patent number: 10542720
    Abstract: A soybean cultivar designated 79252127 is disclosed. The invention relates to the seeds of soybean cultivar 79252127, to the plants of soybean cultivar 79252127, to the plant parts of soybean cultivar 79252127, and to methods for producing progeny of soybean cultivar 79252127. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 79252127. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 79252127, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 79252127 with another soybean cultivar.
    Type: Grant
    Filed: August 8, 2018
    Date of Patent: January 28, 2020
    Assignee: Mertec, LLC
    Inventor: Patrick Joseph Arthur Grams
  • Patent number: 10544397
    Abstract: This present invention relates to a modified mammalian cell in which the genome of the cell is modified to comprise a sequence encoding CP77 under the control of a promoter such that the modified cell line sustains propagation of a poxvirus that is less able or unable to propagate in the unmodified cell.
    Type: Grant
    Filed: November 3, 2014
    Date of Patent: January 28, 2020
    Assignee: SEMENTIS LIMITED
    Inventors: Paul Michael Howley, Liang Liu
  • Patent number: 10538774
    Abstract: Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1X of at least one HPPD inhibitor, and methods for making such plants.
    Type: Grant
    Filed: August 14, 2012
    Date of Patent: January 21, 2020
    Assignee: BASF AGRICULTURAL SOLUTIONS SEED, US LLC
    Inventor: Kathleen D'Halluin
  • Patent number: 10538765
    Abstract: The present disclosure relates to methods of treating EPAS1-related diseases such as cancer, metastases, astrocytoma, bladder cancer, breast cancer, chondrosarcoma, colorectal carcinoma, gastric carcinoma, glioblastoma, head and neck squamous cell carcinoma, hepatocellular carcinoma, lung adenocarcinoma, neuroblastoma, non-small cell lung cancer, melanoma, multiple myeloma, ovarian cancer, rectal cancer, renal cancer, clear cell renal cell carcinoma (and metastases of this and other cancers), gingivitis, psoriasis, Kaposi's sarcoma-associated herpesvirus, preemclampsia, inflammation, chronic imflammation, neovascular diseases, and rheumatoid arthritis, using a therapeutically effective amount of a RNAi agent to EPAS1.
    Type: Grant
    Filed: December 7, 2017
    Date of Patent: January 21, 2020
    Assignee: Arrowhead Pharmaceuticals, Inc.
    Inventors: Brian Bettencourt, Shanthi Ganesh, Elizabeth George, Dieter Huesken, Stuart Milstein, Jonathan Solomon, Emily Thomas, Ivanka Toudjarska, Jennifer Tullai, Jan Weiler
  • Patent number: 10538784
    Abstract: Disclosed herein are transfection complexes comprising at least one cell surface ligand; at least one helper lipid component; and a transfection enhancer. Also disclosed are pharmaceutical compositions comprising the disclosed transfection complexes, and a pharmaceutically acceptable carrier. Further, disclosed are methods of transfecting a cell, the method comprising the steps of: obtaining a transfection complex as disclosed; and contacting a cell with the transfection complex.
    Type: Grant
    Filed: March 1, 2017
    Date of Patent: January 21, 2020
    Assignee: MOLECULAR TRANSFER, INC.
    Inventors: Gulilat Gebeyehu, Joel Jessee
  • Patent number: 10537630
    Abstract: Described herein are improved purification methods for virus vaccines and compositions. Also described are Zika, Chikungunya, dengue and yellow fever vaccines and methods of producing and administering said vaccines to subjects in need thereof.
    Type: Grant
    Filed: December 23, 2016
    Date of Patent: January 21, 2020
    Assignee: Valneva SE
    Inventors: Jana Barbero Calzado, Mario Nebenführ, Robert Schlegl, Michael Weber, Jürgen Heindl-Wruss
  • Patent number: 10531654
    Abstract: The leading cause of graft failure is the subsequent development of intimal hyperplasia, which represents a response to injury that is thought to involve smooth muscle proliferation, migration, phenotypic modulation, and extracellular matrix (ECM) deposition. Surgical techniques typically employed for vein harvest—stretching the vein, placing the vein in low pH, solutions, and the use of toxic surgical skin markers—are shown here to cause injury. The invention therefore provides for non-toxic surgical markers than also protect against stretch-induced loss of functional viability, along with other additives. Devices and compositions for reducing physical stress or protecting from the effects flowing therefrom, also are provided.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: January 14, 2020
    Assignees: VANDERBILT UNIVERSITY, THE UNITED STATES GOVERNMENT AS REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS
    Inventors: Colleen M. Brophy, Padmini Komalavilas, Joyce Cheung-Flynn, Kyle M Hocking, Susan S Eagle
  • Patent number: 10532067
    Abstract: RNA encoding an immunogen is co-delivered to non-immune cells at the site of delivery and also to immune cells which infiltrate the site of delivery. The responses of these two cell types to the same delivered RNA lead to two different effects, which interact to produce a strong immune response against the immunogen. The non-immune cells translate the RNA and express the immunogen. Infiltrating immune cells respond to the RNA by expressing type I interferons and pro-inflammatory cytokines which produce a local adjuvant effect which acts on the immunogen-expressing non-immune cells to upregulate major histocompatibility complex expression, thereby increasing presentation of the translated protein to T cells. The effects on the immune and non-immune cells can be achieved by a single delivery of a single RNA e.g. by a single injection.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: January 14, 2020
    Assignee: GlaxoSmithKline Biologicals S.A.
    Inventors: Andrew Geall, Katrin Ramsauer, Gillis Otten, Christian Walter Mandl
  • Patent number: 10526614
    Abstract: Compositions and methods for modulating root hair production and stress responses in plants are provided.
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: January 7, 2020
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Brian Gregory, Shawn Foley
  • Patent number: 10526370
    Abstract: A pentapeptide associated with integrin receptor ?v?3, which has a sequence of arginine-tryptophan-arginine-asparagine-methionine. The pentapeptide targets tumor cells highly expressing ?v?3, but not tumor cells lowly expressing ?v?3 and normal cells. Accordingly, the pentapeptide is useful in the diagnosis and treatment of cancers.
    Type: Grant
    Filed: October 26, 2015
    Date of Patent: January 7, 2020
    Assignee: CHINA PHARMACEUTICAL UNIVERSITY
    Inventors: Yueqing Gu, Congying Zhang, Menglu Zhao, Qian Wang, Caiping Tian, Yuxi Liu
  • Patent number: 10524493
    Abstract: Food products and/or pharmaceutical preparations including (i) viral neutralizing antibodies or antibody fragments anchored to a probiotic microorganism and (ii) a carrier medium for delivering the viral neutralizing antibodies or antibody fragments anchored to probiotic microorganisms to the gut of a mammal. Also provided are methods of making food products and/or pharmaceutical preparations, which can be used to treat existing viral infections or prevent the spread or transmission of viral infection.
    Type: Grant
    Filed: September 16, 2014
    Date of Patent: January 7, 2020
    Assignee: The Johns Hopkins University
    Inventors: Andrew B. Feldman, Jeffrey S. Lin, Kellogg Schwab, Timothy Julian, Christiane Wobus, David Weitz, John Heyman