Ultraviolet Irradiation Patents (Class 435/448)
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Patent number: 9279115Abstract: Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.Type: GrantFiled: June 7, 2012Date of Patent: March 8, 2016Assignee: NOVOZYMES A/SInventors: Soren Flensted Lassen, Carsten Sjoeholm, Peter Rahbek Øestergaard, Morten Fischer
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Patent number: 9279114Abstract: Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.Type: GrantFiled: June 7, 2012Date of Patent: March 8, 2016Assignee: NOVOZYMES A/SInventors: Soren Flensted Lassen, Carsten Sjoeholm, Peter Rahbek Øestergaard, Morten Fischer
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Patent number: 9029096Abstract: The present invention relates to a method and assay useful for determining the sensitivity of the cells of a subject to genetic damage from electromagnetic radiation. The assay may comprise a substrate suitable for mounting a sample of lymphocytes from a subject and an electromagnetic radiation source.Type: GrantFiled: October 26, 2007Date of Patent: May 12, 2015Assignee: The University of BradfordInventors: David Thomas Sharpe, Morgan Clive Thomas Denyer, Diana Anderson, Stephen Thomas Britland, Rajendran Gopalan
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Publication number: 20140342408Abstract: Provided are isolated polypeptides having xylanase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: November 22, 2012Publication date: November 20, 2014Inventors: Yu Zhang, Lan Tang, Junxin Duan, Ye Liu
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Patent number: 8871493Abstract: A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of Chrysosporium lucknowense and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.Type: GrantFiled: September 17, 2012Date of Patent: October 28, 2014Assignee: Dyadic International (USA), Inc.Inventors: Mark Aaron Emalfarb, Richard Paul Burlingame, Philip Terry Olson, Arkady Panteleimonovich Sinitsyn, Martine Parriche, Jean Christophe Bousson, Christine Marie Pynnonen, Peter Jan Punt, Cornelia Marie Johanna Van Zeijl
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Patent number: 8865450Abstract: The present invention relates to a method for production of continuous cell lines comprising providing living cells of an animal or a human, irradiating said cells with UV light, proliferating said cells and selecting multiplying cells as cells of a continuous cell line.Type: GrantFiled: February 20, 2009Date of Patent: October 21, 2014Assignees: Baxter International Inc., Baxter Healthcare S.A.Inventors: Manfred Reiter, Wolfgang Mundt, Simone Feigl, Simone Von Fircks
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Publication number: 20140308705Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 20, 2012Publication date: October 16, 2014Inventors: Marc Morant, Tarana Shaghasi
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Publication number: 20140113304Abstract: The present invention relates to a bi-directional cytosine deaminase-encoding selection marker as well as methods for using said marker.Type: ApplicationFiled: May 23, 2012Publication date: April 24, 2014Applicant: Novozymes A/SInventor: Hiroaki Udagawa
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Patent number: 8609419Abstract: The invention concerns the use of a mutagenic agent blocking DNA replication in the cell for inserting in vitro a nucleic acid of interest inside a predetermined nucleotide sequence present in a chromosome contained in a prokaryotic or eukaryotic cell, said nucleic acid of interest being, prior to its insertion, included in a DNA vector which replicates in said prokaryotic or eukaryotic host cell.Type: GrantFiled: October 27, 2003Date of Patent: December 17, 2013Assignee: Centre National de la Recherche ScientifiqueInventors: Robert Fuchs, Marc Bichara
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Patent number: 8609382Abstract: Described are novel S. stipitis strains that were obtained by UV-C irradiation of wild-type S. stipitis NRRL Y-7124 cultures, followed by 5-month anaerobic growth on xylose at 28° C. The UV-C-mutagenized strains were able to grow anaerobically on xylose or glucose medium with higher ethanol production than a Saccharomyces cerevisiae yeast strain under comparable fermentation conditions. The mutagenized strains were identified by DNA fingerprinting to be unique strains closely related to wild-type Scheffersomyces stipitis. These mutagenized strains have potential application in large-scale industrial conversion of lignocellulosic sugars to fuel ethanol.Type: GrantFiled: August 1, 2012Date of Patent: December 17, 2013Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: Stephen R. Hughes, William R. Gibbons
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Publication number: 20130302373Abstract: The present invention provides live, attenuated Mycoplasma gallisepticum bacteria that exhibit reduced expression of a protein identified as MGA_0621. In certain embodiments, the attenuated bacteria may additionally exhibit reduced expression of one or more proteins selected from the group consisting of pyruvate dehydrogenase, phosphopyruvate hydratase, 2-deoxyribose-5-phosphate aldolase, and ribosomal protein L35, relative to a wild-type M. gallisepticum bacterium. Also provided are vaccines and vaccination methods involving the use of the live, attenuated M. gallisepticum bacteria, and methods for making live attenuated M. gallisepticum bacteria. An exemplary live, attenuated strain of M. gallisepticum is provided, designated MGx+47, which was shown by proteomics analysis to exhibit significantly reduced expression of MGA_0621, and was shown to be safe and effective when administered as a vaccine against M. gallisepticum infection in chickens.Type: ApplicationFiled: July 25, 2013Publication date: November 14, 2013Inventors: Mahesh KUMAR, Muhammad Ayub KHAN
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Patent number: 8349598Abstract: A method of optoperforation of the membrane of a cell by application of laser pulses characterized by focusing the pulsed laser beam onto the cell membrane to be perforated, applying a series of laser pulses of predetermined pulse energy, measuring the oscillation time of the bubbles formed in the laser focus from the change in laser intensity of a test laser beam transmitted through the laser focus and caused by the bubbles in the laser focus, and increasing the pulse energy to a level at which the oscillation time of the bubbles attains a predetermined value.Type: GrantFiled: January 9, 2008Date of Patent: January 8, 2013Assignee: Universitat Zu LubeckInventor: Alfred Vogel
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Publication number: 20120202706Abstract: Disclosed are methods of 6-O sulfating glucosaminyl N-acetylglucosamine residues (GlcNAc) in a polysaccharide preparation and methods of converting anticoagulant-inactive heparan sulfate to anticoagulant-active heparan sulfate and substantially pure polysaccharide preparations made by such methods. Also disclosed is a mutant CHO cell which hyper-produces anticoagulant-active heparan sulfate. Methods for elucidating the sequence of activity of enzymes in a biosynthetic pathway are provided.Type: ApplicationFiled: November 29, 2011Publication date: August 9, 2012Inventors: Robert D. Rosenberg, Lijuan Zhang, David L. Beeler
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Publication number: 20120083036Abstract: The present invention is predicated on the discovery that a polysaccharide transferase purified from barley seedlings can catalyze the formation of covalent bonds between different polysaccharides, including between xyloglucans and cellulose, and between xyloglucans and (1,3;1,4)-?-D-glucans. The present invention thus provides, among other things, an isolated or substantially purified polysaccharide transferase, wherein said polysaccharide transferase is capable of catalyzing the formation of a covalent bond between a donor polysaccharide and an acceptor polysaccharide; or a functionally active fragment or variant of said polysaccharide transferase.Type: ApplicationFiled: December 14, 2011Publication date: April 5, 2012Applicant: ADELAIDE RESEARCH AND INNOVATION PTY LTDInventors: Maria Hrmova, Geoffrey Bruce Fincher
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Patent number: 8101386Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacteria strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: GrantFiled: June 28, 2010Date of Patent: January 24, 2012Assignee: Archer Daniels Midland CompanyInventors: Hungming J. Liaw, Jill S. Bradshaw, Yueqin Yang, Weiying Mao
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Publication number: 20110294979Abstract: The present invention relates to compositions and methods to identify novel bacteria and metabolites derived therefrom. More specifically, the invention describes a novel method to isolate bacteria producing metabolites of interest from environmental samples. Particularly, the invention discloses a method to select rare antibiotic producing bacteria. The invention can be used from any sample and allows the isolation of bacteria having e.g., pharmaceutical or agrochemical interest.Type: ApplicationFiled: January 18, 2010Publication date: December 1, 2011Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, DEINOVEInventors: Jean-Paul Leonetti, Stephanie Texier
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Publication number: 20100311612Abstract: The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multicellular organisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create knock-outs, knock-downs, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses.Type: ApplicationFiled: January 13, 2010Publication date: December 9, 2010Applicant: ABT HOLDING COMPANYInventors: John Joseph Harrington, Paul David Jackson, Li Jiang
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Patent number: 7767431Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacteria strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: GrantFiled: April 13, 2007Date of Patent: August 3, 2010Assignee: Archer-Daniels-Midland CompanyInventors: Hungming J. Liaw, Jill S. Bradshaw, Yueqin Yang, Weiying Mao
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Patent number: 7759121Abstract: Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be rendered genetically stable by restoring the mismatch repair system to proper functionality.Type: GrantFiled: October 31, 2007Date of Patent: July 20, 2010Assignees: The John Hopkins University, Morphotek, Inc.Inventors: Nicholas C. Nicolaides, Philip M. Sass, Luigi Grasso, Bert Vogelstein, Kenneth W. Kinzler
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Patent number: 7687253Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacteria strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: GrantFiled: March 19, 2008Date of Patent: March 30, 2010Assignee: Archer-Daniels-Midland CompanyInventors: Hungming J. Liaw, Jill S. Bradshaw, Yueqin Yang, Weiying Mao
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Publication number: 20100009406Abstract: The present invention relates to methods, reagents and kits for detecting of formyl peptide receptor like-2 (FPRL2) polypeptide activity in a sample and identifying agents which modulate polypeptide activity. It further relates to antibodies raised against FPRL2. It further relates to substances for preventing, treating and/or alleviating diseases or disorders characterized by dysregulation of FPRL2 polypeptide signalling.Type: ApplicationFiled: March 13, 2008Publication date: January 14, 2010Inventors: Isabelle Migeotte, Marc Parmentier, David Communi, Maryse Brait
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Publication number: 20090298151Abstract: The disclosed invention relates to an isolated hydrogen gas producing microorganism, termed Enterobacter sp. SGT-T4™ and derivatives thereof. Compositions and methods comprising the disclosed microorganisms are also provided.Type: ApplicationFiled: June 3, 2008Publication date: December 3, 2009Applicant: Dr. Elmar Schmid and James GibsonInventors: Elmar Schmid, James Gibson
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Publication number: 20090186390Abstract: A method is described to construct genetically modified strains of steroid degrading micro-organisms wherein the method comprises inactivation of at least one gene involved in methylhexahydroindanedione propionate degradation. Strains with (multiple) inactivated steroid degrading enzyme genes according to the invention can be used in the accumulation of steroid intermediates. Accumulation products are for example 3a?-H-4?(3?-propionic acid)-7a?-methylhexahydro-1,5-indanedione (HIP), 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione (3-HSA), 1,4-androstadiene-3,17-dione (ADD) and 3a?-H-4?(3?-propionic acid)-5?-hydroxy-7a?-methylhexahydro-1-indanone-?-lactone (HIL).Type: ApplicationFiled: August 20, 2008Publication date: July 23, 2009Inventors: Robert van der Geize, Gerda Hessels, Lubbert Dijkhuizen
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Publication number: 20090155411Abstract: Mutants of lactic acid bacteria including Lactococcus lactis which are defective in pyruvate formate-lyase production and/or in their lactate dehydrogenase (Ldh) production and methods of isolating such mutants or variants are provided. The mutants are useful in the production of food products or in manufacturing of compounds such as diacetyl, acetoin and acetaldehyde and as components of food starter cultures.Type: ApplicationFiled: December 11, 2008Publication date: June 18, 2009Inventor: Dan Nilsson
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Publication number: 20090136624Abstract: The present invention provides lactic acid bacteria producing Nisin, a method for selecting the same, and foods or feeds using the lactic acid bacteria.Type: ApplicationFiled: January 26, 2009Publication date: May 28, 2009Applicant: Ajinomoto Co., Inc.Inventors: Naoko TANAKA, Hiroaki NISHIUCHI, Akinori UEHARA, Nobutoshi MATSUMOTO
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Patent number: 7524327Abstract: In accordance with the present invention, methods are provided for treating a patient through the use of ultraviolet light activated gene therapy. Embodiments of the present invention include methods for the utilization of light activated gene therapy to repair and/or rebuild damaged cartilage by introducing a desired gene into a patient's tissue.Type: GrantFiled: January 31, 2003Date of Patent: April 28, 2009Assignee: University of RochesterInventors: Edward M. Schwarz, Regis J. O'Keefe, Thomas Foster, Jarod C. Finlay
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Publication number: 20090029433Abstract: The disclosed invention relates to an isolated hydrogen gas producing microorganism, termed Enterobacter sp. SGT 06-1™.Type: ApplicationFiled: July 27, 2007Publication date: January 29, 2009Applicant: Dr. Elmar Schmid and James GibsonInventors: Elmar Schmid, James Warner Gibson
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Publication number: 20080176329Abstract: Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be rendered genetically stable by restoring the mismatch repair system to proper functionality.Type: ApplicationFiled: October 31, 2007Publication date: July 24, 2008Applicants: The Johns Hopkins University, Morphotek, Inc.Inventors: Nicholas C. Nicolaides, Philip M. Sass, Luigi Grasso, Bert Vogelstein, Kenneth W. Kinzler
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Patent number: 7220571Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacterial strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: GrantFiled: September 26, 2001Date of Patent: May 22, 2007Assignee: Archer-Daniels-Midland CompanyInventors: Hungming J Liaw, Jill S Bradshaw, Yueqin Yang, Weiying Mao
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Patent number: 7208317Abstract: Cellular libraries useful for in vitro phenotyping and gene mapping. In a representative approach, a method for preparing a homozygous cellular library includes the steps of providing a heterozygous cellular library comprising a plurality of isolated parent cells; inducing site-specific mitotic recombination in the plurality of isolated parent cells; culturing the plurality of isolated parent cells, whereby a population of daughter cells is produced; and selecting daughter cells comprising a homozygous genetic modification, whereby a homozygous cellular library is prepared.Type: GrantFiled: May 2, 2003Date of Patent: April 24, 2007Assignee: The University of North Carolina at Chapel HillInventors: David W. Threadgill, Daekee Lee
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Patent number: 7132285Abstract: Treatment and/or prophylaxis, in mammalian patients, of neurodegenerative and other neurological medical disorders is effected by administering to the patient effective amounts of apoptotic bodies and/or apoptotic cells, preferably those derived from the patient's own white blood cells, e.g. by extracorporeal treatment of the patient's blood cells to induce apoptosis and administration of the apoptotic bodies and/or cells so formed to the patient.Type: GrantFiled: May 25, 2001Date of Patent: November 7, 2006Assignee: Vasogen Ireland LimitedInventors: Anthony E. Bolton, Arkady Mandel, Daniel Sauder
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Patent number: 7105292Abstract: The cellular response to ultraviolet radiation exposure has been characterized on the molecular level through the use of high density gene array technology. Nucleic acid molecules and protein molecules, the expression of which are repressed or induced in response to ultraviolet radiation exposure, are identified according to a temporal pattern of altered expression post ultraviolet radiation exposure. Methods are disclosed that utilized these ultraviolet radiation-regulated molecules as markers for ultraviolet radiation exposure. Other screening methods of the invention are designed for the identification of compounds that modulate the response of a cell to ultraviolet radiation exposure. The invention also provides compositions useful for drug screening or pharmaceutical purposes.Type: GrantFiled: September 6, 2001Date of Patent: September 12, 2006Assignee: New York UniversityInventor: Miroslav Blumenberg
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Patent number: 6843961Abstract: Methods and apparatuses are provided for inactivation of pathogens in fluids containing blood products. Preferred methods include the steps of adding an effective, nontoxic amount of a photosensitizer such as riboflavin to the blood product and exposing the fluid to light having a peak wavelength.Type: GrantFiled: February 3, 2003Date of Patent: January 18, 2005Assignee: Gambro, Inc.Inventors: Dennis J. Hlavinka, Raymond P. Goodrich, Laura Goodrich, Daniel McGraw
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Patent number: 6835867Abstract: The present invention relates to methods of producing an allelic series of modifications in genes of interest in a cell. In particular, the invention provides methods for using nucleic acid sequence-modifying agents (e.g., chemicals, electromagnetic radiation, etc.) to introduce modifications in any nucleic acid sequence in the genome of a cell. Also provided are sets of cells which contain at least one modification in any gene of interest. The methods and compositions of the invention are useful in determining the function of the gene of interest.Type: GrantFiled: June 24, 1998Date of Patent: December 28, 2004Inventors: Richard P. Woychik, Terry R. Magnuson, Ellis D. Avner, James W. Thomas
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Publication number: 20030126642Abstract: The present invention provides compositions and methods for modulating plant development by modulating the expression or activity of plant polycomb genes including FIE and MEA.Type: ApplicationFiled: June 21, 2002Publication date: July 3, 2003Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Robert Fischer, Tetsu Kinoshita, Ramin Yadegari, Mary Gehring, Jack Okamuro, Van-Dinh Dang
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Publication number: 20030113759Abstract: Disclosed is a process of performing “sexual” PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis or amplification of at least one polynucleotide, optionally amplifying the polynucleotides, and reannealing the polynucleotides to produce random mutant polynucleotides. Also provided are vector and expression vehicles including such mutant polynucleotides, polypeptides expressed by the mutant polynucleotides and a method for producing random mutant polypeptides.Type: ApplicationFiled: August 12, 2002Publication date: June 19, 2003Inventor: Jay M. Short
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Publication number: 20030064365Abstract: The present invention provides a method for detecting heterozygous mutation using E. coli stop codon assay. The present invention further provides a gap vector used in the E. coli stop codon assay. According to this invention, the heterozygous mutation in certain gene, e.g. APC gene or BRCA1 gene, may be detected in simple and rapid manner, for example, visual observation on colonies.Type: ApplicationFiled: June 26, 2001Publication date: April 3, 2003Inventors: Young-Ho Moon, Hye-Jung Nam, Dong-Hwan Kim, Hyun-Pil Cho, Su-Mi Han, Mi-Uk Chin, Seg-Ho Choi, Su-Yun Kim, Sang-Yong Choi, Byung-Joo Song, Eun-Ryoung Kim, Hyo-Jong Kim, Il-Soo Kim
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Publication number: 20020197633Abstract: The present invention describes a method for treating and/or evaluating photodamage and/or photoaging of skin caused by exposure to solar ultraviolet (UV) radiation. The method employs a unique set of marker genes whose expression was newly found to be altered following exposure of skin to UV radiation. The invention provides an advantageous system of identifying and assessing substances that are capable of modulating, e.g., via attenuation, UV radiation induced alteration or change in the expression of at least one of the newly provided marker genes in skin relative to the gene expression level in skin not exposed to UV radiation. Also provided are compositions comprising materials that upon application to skin can modulate the gene expression of at least one gene of the marker gene set after exposure of skin to UV radiation, thereby affording protective and therapeutic effects and treatments for photodamage and photoaging. The potential benefit of, e.g.Type: ApplicationFiled: May 9, 2002Publication date: December 26, 2002Inventors: Brian C. Jones, Carol Bosko, Kevin Cooper, Thomas McCormick
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Publication number: 20020155606Abstract: A target gene can be expressed site-specifically and with desired timing without damaging the cell itself or inducing mutation in a nucleic acid molecule within the cell. Expression of the above target gene is suppressed by allowing a caging agent represented by the following formula (1) to bind to a nucleic acid molecule containing the target gene; and the suppression of expression of the above target gene is cancelled by detaching the caging agent from the above nucleic acid molecule by UV-illumination.Type: ApplicationFiled: April 22, 2002Publication date: October 24, 2002Inventors: Hitoshi Okamoto, Hideki Ando, Toshiaki Furuta
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Publication number: 20020142465Abstract: A method for processing a cell, including the steps of irradiating a cell or a living tissue with a laser beam through an optical fiber, and cutting off, removing or boring a cell wall and/or a cell membrane or an entirety of the cell thus irradiated.Type: ApplicationFiled: January 10, 2002Publication date: October 3, 2002Applicant: OSAKA UNIVERSITYInventors: Akio Kobayashi, Kiichi Fukui, Satoshi Harajima, Eiichiro Fukusaki, Shinichiro Kajiyama, Shinya Okuda, Takeshi Shoji
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Patent number: 6455257Abstract: An apparatus suitable for making a designer disease is disclosed. The apparatus comprises first, second, and third containers, each having two sections, I and II, separated by a semipermeable material through which a microbe can pass but cells from target and non-target populations cannot pass, and an entrance and an exit to each section. The entrance to section I of the first and second containers are circulation entrances, the exits from section II of the first and second containers are circulation exits, and the entrance to and exit from section I of the third container is a circulation entrance and a circulation exit, respectively. Conduits form a loop by connecting the circulation exit of each container to the circulation entrance to another container. A pump moves fluid around the loop and microbes in the fluid are mutated. A method of making a designer disease using that apparatus is also disclosed.Type: GrantFiled: February 16, 2001Date of Patent: September 24, 2002Inventor: Richard D. Fuerle
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Publication number: 20020119569Abstract: An apparatus is disclosed for denaturing DEOXYRIBONUCLEIC ACID and fragments thereof from laboratory equipment. The apparatus includes a chamber which defines an enclosure. The chamber has an opening for gaining access to the enclosure. A closure is provided for selectively closing the opening. The arrangement is such that when the closure is disposed in a first location thereof relative to the enclosure, the enclosure is sealed and when the closure is disposed in a second location thereof relative to the enclosure, access to the enclosure is permitted. A liner is disposed within the enclosure, the liner having an inside surface, the inside surface of the liner being reflective. An emitter is disposed within the liner, the emitter being selectively connected to a source of electrical power. The arrangement is such that in use of the apparatus, when the emitter is connected to the source of power, ultraviolet radiation is emitted from the emitter.Type: ApplicationFiled: February 26, 2001Publication date: August 29, 2002Inventor: John C. Menz
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Publication number: 20020114793Abstract: A method for inducing differentiation of monocytes contained in an extracorporeal quantity of a subject's blood into functional dendritic antigen presenting cells is provided. The monocytes are induced to differentiate into dendritic cells by activation forces resulting from flow of the monocytes through a plastic channel, such as the plastic channel in a conventional photopheresis apparatus. Functional dendritic cells generated from induced monocytes are incubated together with apoptotic or inactivated disease effector agents to enhance the presentation of at least one disease-causing antigen expressed by the disease effector agents. Compositions including dendritic cells derived from induced monocytes and compositions including such dendritic cells incubated with disease effector agents are also provided for use in immunotherapeutic treatment.Type: ApplicationFiled: January 31, 2002Publication date: August 22, 2002Inventors: Richard Leslie Edelson, Carole Berger, Douglas Hanlon
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Publication number: 20020106800Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacterial strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: ApplicationFiled: September 26, 2001Publication date: August 8, 2002Inventors: Hungming J. Liaw, Jill S. Bradshaw, Yueqin Yang, Weiying Mao
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Patent number: 6413765Abstract: Genetically modified lactic acid bacteria having a reduced or lacking or enhanced diacetyl reductase activity, acetoin reductase activity and/or butanediol dehydrogenase activity are provided. Such bacteria are used in starter cultures in the production of food products including dairy products where it is desired to have a high content of diacetyl and for reducing or completely removing diacetyl in beverages including beers, fruit juices and certain types of wine, where the presence of diacetyl is undesired.Type: GrantFiled: April 21, 1999Date of Patent: July 2, 2002Assignee: Chr. Hanson A/SInventors: Mats Walfridsson, Claus Maxel Henriksen, Dan Nilsson
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Publication number: 20010028879Abstract: The development of graft versus host disease in a mammalian patient undergoing cell transplantation therapy for treatment of a bone marrow mediated disease, is prevented or alleviated by subjecting at least the T-cells of the allogeneic cell transplantation composition, extracorporeally, to oxidative stress, in appropriate dosage amounts, such as bubbling a gaseous mixture of ozone and oxygen through a suspension of the T-cells. The process may also include irradiation of the cells with UV light, simultaneously with the application of the oxidative stress. The oxidative stress induces reduced inflammatory cytokine production and a reduced proliferative response in the T-cells.Type: ApplicationFiled: May 14, 2001Publication date: October 11, 2001Inventor: David Elliott Spaner
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Patent number: 6204006Abstract: Microbial strains capable of producing enhanced levels of sphingosine, dihydrosphingosine, phytosphingosine and/or derivatives thereof are disclosed. Additionally, there are disclosed methods based on mutagenesis, or other selection techniques, whereby such strains can be produced. As a preferred example thereof, mutant strains of Pichia are provided that are capable of producing about 50% or more of such compounds than wild type strains.Type: GrantFiled: April 7, 1998Date of Patent: March 20, 2001Assignee: DSM, N.V.Inventors: Lex De Boer, Ingrid Francisca Caroline Van Der Wildt
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Patent number: 6204058Abstract: An autoimmune vaccine is provided for administration to human patients to alleviate the symptoms of autoimmune diseases such as rheumatoid arthritis. The vaccine comprises an aliquot of the patient's blood, containing, inter alia, leukocytes having upregulated expression of various cell surface markers and lymphocytes containing decreased amounts of certain stress proteins. It is produced by subjecting the blood aliquot extracorporeally to certain stressors, namely oxidizing agents, UV radiation and elevated temperature.Type: GrantFiled: December 16, 1999Date of Patent: March 20, 2001Assignee: Vasogen Ireland LimitedInventor: Anthony E. Bolton
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Patent number: 6132989Abstract: The invention provides compositions of a non-adenoviral vector containing a polynucleotide sequence encoding adenoviral pTP operationally linked domain. The invention also provides compositions of an adenoviral pTP binding domain. The invention also provides methods for increasing the expression of a polynucleotide by expressing the polynucleotide in a non-adenoviral vector containing an adenoviral pTP binding domain in the presence of adenoviral pTP. The invention additionally provides methods to increase expression of a heterologous polynucleotide in an individual by obtaining cells from the individual, genetically altering the cells to express a non-adenoviral vector containing an adenoviral pTP binding domain and a gene encoding pTP and readministering the genetically altered cells to the individual.Type: GrantFiled: November 18, 1997Date of Patent: October 17, 2000Assignee: University of WashingtonInventors: Mark A. Kay, Andre Lieber
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Patent number: 5928866Abstract: This invention provides A method for preparing mutant genes, which comprises the steps of constructing a recombinant plasmid DNA by inserting a gene fragment into a plasmid DNA having a unidirectional origin, introducing the recombinant plasmid DNA into host cell lacking DNA error-correcting function to transform the host cell, and culturing the transformant cell in a condition that any mutations of the inserted gene is detectable. According to the present invention, diverse mutant genes can be prepared in a short period of time.Type: GrantFiled: December 15, 1995Date of Patent: July 27, 1999Assignee: Research Development Corporation of JapanInventors: Fumio Imamoto, Yoshizumi Ishino, Mitsuru Furusawa, Hirofumi Doi