Involving Particle-mediated Transfection (i.e., Biolistic Transfection) Patents (Class 435/459)
  • Patent number: 10471254
    Abstract: A system and method for selectively treating aberrant cells such as cancer cells through administration of a train of electrical pulses is described. The pulse length and delay between successive pulses is optimized to produce effects on intracellular membrane potentials. Therapies based on the system and method produce two treatment zones: an ablation zone surrounding the electrodes within which aberrant cells are non-selectively killed and a selective treatment zone surrounding the ablation zone within which target cells are selectively killed through effects on intracellular membrane potentials. As a result, infiltrating tumor cells within a tumor margin can be effectively treated while sparing healthy tissue. The system and method are useful for treating various cancers in which solid tumors form and have a chance of recurrence from microscopic disease surrounding the tumor.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: November 12, 2019
    Assignee: Virginia Tech Intellectual Properties, Inc.
    Inventors: Michael B. Sano, Christopher B. Arena, Scott S. Verbridge, Rafael V. Davalos
  • Patent number: 10421938
    Abstract: The invention relates to a novel plasma induced mutation breeding device which comprises: a sample treatment system including a sterile working compartment free of bioactive contaminant; a plasma generator; a radio frequency (RF) power module connected with the plasma generator; a cooling system for cooling the plasma generator; a detection system including a gas flow controller for controlling the gas flow which generates the plasma jet and a temperature sensor for detecting the temperature of the jet emitted by the plasma generator; and a control system with an operation panel and a controller for controlling the operation of the mutation breeding device, wherein the controller is connected with the RF power module, gas flow controller, temperature sensor, cooling system as well as the operation panel, respectively, and said plasma generator stably emits the plasma jet at 37±3° C. during the biological sample processing.
    Type: Grant
    Filed: March 2, 2017
    Date of Patent: September 24, 2019
    Assignee: Luoyang TMAXTREE Biotechnology Co., Ltd
    Inventors: Liyan Wang, Xianrong Bi
  • Patent number: 10422770
    Abstract: In at least one illustrative embodiment, a system may include a basin that includes an index plate positioned at a bottom of the basin. The basin is configured to receive a liquid analyte, such as a liquid food product or a nutrient broth. The index plate includes an array of multiple wells. Each well opens into an interior of the basin and is sized to receive a magnetostrictive sensor in a predetermined orientation. One or more sensor coils is positionable beneath each well. The basin may be filled with liquid analyte and magnetostrictive sensors may be positioned in the wells. The liquid analyte may be allowed to incubate at a controlled temperature. A controller may position a sensor coil beneath a well, apply a varying magnetic field to a magnetostrictive sensor in the well, and detect a frequency response of the magnetostrictive sensor. Other embodiments are described and claimed.
    Type: Grant
    Filed: June 23, 2017
    Date of Patent: September 24, 2019
    Assignee: Auburn University
    Inventors: Bryan A. Chin, Shin Horikawa, Zhongyang Cheng
  • Patent number: 9937272
    Abstract: A water-soluble compound of the formula (I): wherein R9 and R10 are suitably hydrophilic substituents, which may be used to selectively bind to a target saccharide such as glucose and which exhibits a detectable spectroscopic response to such binding, thus enabling its use in the detection and correction of blood glucose concentrations in vivo.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: April 10, 2018
    Assignee: University of Bristol
    Inventors: Chenfeng Ke, Anthony P. Davis
  • Patent number: 9895451
    Abstract: Polyamine-co-ester-co-ortho ester) polymers, methods of forming active agent-load nanoparticles therefrom, and methods of using the nanoparticles for drug delivery are disclosed. The nanoparticles can be coated with an agent that reduces surface charge, an agent that increases cell-specific targeting, or a combination thereof. Typically, the loaded nanoparticles are less toxic, more efficient at drug delivery, or a combination thereof compared to a control or other transfection reagents.
    Type: Grant
    Filed: November 18, 2014
    Date of Patent: February 20, 2018
    Assignee: Yale University
    Inventors: W. Mark Saltzman, Junwei Zhang, Jiangbing Zhou, Zhaozhong Jiang
  • Patent number: 9694368
    Abstract: The present invention relates to a magnetic reagent, a magnetic reagent kit, a method for treating magnetic carriers, and a treatment device therefor, with an object of quickly and efficiently treating magnetic carriers of a micro particle diameter including nanosized magnetic carriers. The magnetic reagent or magnetic reagent kit includes: a plurality of magnetic carriers which can be magnetized by being exposed to a magnetic field, can be bonded to a predetermined chemical substance or living organism in a liquid, and that have a particle diameter that enables them to be suspended in the liquid; and a plurality of treatment promoting magnetic particles which can be magnetized by being exposed to a magnetic field and thus can have the magnetic carriers adsorbed on the surface thereof, are formed so that they can be moved within the liquid by movement of the liquid or by a magnetic field, and promote a treatment for capturing or re-suspension of the magnetic carriers.
    Type: Grant
    Filed: November 12, 2010
    Date of Patent: July 4, 2017
    Assignee: UNIVERSAL BIO RESEARCH CO., LTD.
    Inventor: Hideji Tajima
  • Patent number: 9540246
    Abstract: A method of making porous silicon using a chemical etchant comprising metal ions is described.
    Type: Grant
    Filed: December 16, 2010
    Date of Patent: January 10, 2017
    Assignee: PSIMEDICA LIMITED
    Inventors: Leigh Trevor Canham, Armando Loni
  • Patent number: 9327980
    Abstract: Alterations utilizing nanoparticles. Certain embodiments of the invention are methods of delivering a substance to a target using a delivery-aid which includes nanoparticles. Those nanoparticles may be nanocarbon particles. Other embodiments are methods of delivering nanoparticles to a target involving placing a mask between a source of ballistic delivery of nanoparticles and the target. Other embodiments include irradiating a target to cause localized heating of the region of the target in which the nanodiamonds or OLC particles are present. Other embodiments utilize nanoparticles to make cells competent for genetic transformation. This abstract is not to be considered limiting, since other embodiments may deviate from the features described in this abstract.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: May 3, 2016
    Assignee: International Technology Center
    Inventors: Varvara P. Grichko, Olga Alexander Shenderova
  • Publication number: 20150073041
    Abstract: Polyamine-co-ester-co-ortho ester) polymers, methods of forming active agent-load nanoparticles therefrom, and methods of using the nanoparticles for drug delivery are disclosed. The nanoparticles can be coated with an agent that reduces surface charge, an agent that increases cell-specific targeting, or a combination thereof. Typically, the loaded nanoparticles are less toxic, more efficient at drug delivery, or a combination thereof compared to a control or other transfection reagents.
    Type: Application
    Filed: November 18, 2014
    Publication date: March 12, 2015
    Inventors: W. Mark Saltzman, Junwei Zhang, Jiangbing Zhou, Zhaozhong Jiang
  • Publication number: 20140295558
    Abstract: The present invention is concerned with a system and method for introducing a substance into cells. The system has an assembly including a plurality of elongate non-hollow nanoneedles forming a nanoneedle array or patch for delivering the substance into the cells, at least some of the nanoneedles have a non-uniform diameter with a wider upper end, a narrower lower end for penetration into the cells and a length from substantially 200 nm to 100 um. The lower end has a diameter from substantially 20-436 nm. Adjacent nanoneedles are spaced apart by substantially 5-50 um. The nanoneedles are made from a material selected from the group consisting of diamond, cubic boron nitride, carbon nitride, boron nitride, boron carbon nitride, metal borides and essentially boron materials, allowing the nanoneedles to maintain sufficient thinness and yet adequate rigidity during penetration. The nanoneedles are applied onto the cells grown on substrates at a preferred rate from 1 to 5 m/s.
    Type: Application
    Filed: January 23, 2014
    Publication date: October 2, 2014
    Applicant: CITY UNIVERSITY OF HONG KONG
    Inventors: Xianfeng CHEN, Wenjun ZHANG
  • Patent number: 8834896
    Abstract: Methods and compositions are provided herein for immunizing a subject by administering to the subject an effective amount of an immunogenic peptide or an immunogenic fragment or variant thereof incorporated within a vault-like particle carrier. The methods and compositions advantageously exhibit enhanced ability to induce cell-mediated immunity and/or antibody-based immunity.
    Type: Grant
    Filed: January 10, 2013
    Date of Patent: September 16, 2014
    Assignee: The Regents of the University of California
    Inventors: Valerie A. Kickhoefer, Leonard H. Rome, Kathleen A. Kelly, Cheryl I. Champion
  • Publication number: 20140189899
    Abstract: The present invention provides a new and improved method for producing hybrid silk and like fibers. The invention provides a method of sequence of use which has many advantages over prior art.
    Type: Application
    Filed: January 3, 2013
    Publication date: July 3, 2014
    Inventor: Sadia "Ross" Barrameda
  • Patent number: 8742202
    Abstract: Methods for the transformation of sugar cane are provided. The methods comprise utilizing sugar cane immature shoots as the source of plant material for transformation. Segments of the immature shoot are excised and transformed by any suitable transformation methodology. In some embodiments, the segments are cultured in embryogenic culture induction medium prior to transformation. Transformation can be performed via Agrobacterium-mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: June 3, 2014
    Assignee: Syngenta Participations AG
    Inventor: Heng Zhong
  • Publication number: 20140106456
    Abstract: The invention relates to an apparatus for introducing a biological material, a method of introducing a biological material, and a magnetic support for introducing a biological material with the object of providing an apparatus for introducing a biological material, a method of introducing a biological material, and a magnetic support for introducing a biological material whereby a biological material can be efficiently introduced into a host. The invention comprises: one or more packing units in which a mixture solution containing a large number of magnetic supports carrying a biological material to be introduced into a host such as cells upon using, together with a large number of the hosts in a liquid is pooled; and an introduction treatment unit in which a magnetic force affecting the inside of the packing unit is controlled so as to move the magnetic supports relatively with respect to the host so that the biological material can be introduced into the host.
    Type: Application
    Filed: October 9, 2013
    Publication date: April 17, 2014
    Applicants: UNIVERSAL BIO RESEARCH CO., LTD.
    Inventors: Hideji TAJIMA, Yoshiro OKAMI
  • Publication number: 20140096284
    Abstract: The invention provides particles and methods to deliver freeze- or air-dried molecules to cells.
    Type: Application
    Filed: March 5, 2013
    Publication date: April 3, 2014
    Inventors: Susana Martin-Ortigosa, Kan Wang
  • Publication number: 20130280750
    Abstract: The present disclosure describes methods of maintaining the phenotype of differentiated cells. Generally, the natural environment of the body is replicated for the differentiated cell. The differentiated cell is plated on a cell culture substrate comprising a laminin, such as laminin-521 or laminin-511. The substrate may also contain a cadherin. This maintains the phenotype of the differentiated cell.
    Type: Application
    Filed: April 19, 2013
    Publication date: October 24, 2013
    Applicant: BIOLAMINA AB
    Inventors: Karl Tryggvason, Karl Kristian Tryggvason, Anna Domogatskaya
  • Patent number: 8524368
    Abstract: The present invention provides dynamic charge state cationic polymers that are useful for delivery of anionic molecules. The dynamic charge state cationic polymers are designed to have cationic charge densities that decrease by removal of removable functional groups from the polymers. The present invention also provides interpolyelectrolyte complexes containing the polymers complexed to a polyanion. Methods for using the interpolyelectrolyte complexes to deliver anionic compounds are also provided.
    Type: Grant
    Filed: December 14, 2011
    Date of Patent: September 3, 2013
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David M. Lynn, Adam D. Miller
  • Patent number: 8513019
    Abstract: A fluid containing cells and free genetic material is acoustically coupled to a propulsion surface of a diaphragm. A blast-receiving surface of the diaphragm is acoustically coupled to an explosion chamber in which an explosive material is disposed. An ignition system ignites the explosive material in the explosion chamber to create a blast wave. The diaphragm transfers momentum from the blast wave to the fluid containing cells and free genetic material sufficient to cause the cells to take up the free genetic material.
    Type: Grant
    Filed: July 27, 2012
    Date of Patent: August 20, 2013
    Assignee: Indian Institute of Science
    Inventor: Gopalan Jagadeesh
  • Publication number: 20130184332
    Abstract: The present invention refers to the construction of cloning vectors containing the max gene. Especially, the present invention refers to the introduction of cloning vectors containing the max gene in cells using transport vectors. In addition, the presence of cloning vectors containing the max gene in cells allows the differential expression of the max gene in the same cells. In addition, the present invention refers to a method of gene therapy in which the differential expression of the max gene has cytoprotective activity, especially neuroprotective activity, and is capable of application to medical and veterinary therapeutics of neurodegenerative conditions.
    Type: Application
    Filed: November 21, 2012
    Publication date: July 18, 2013
    Applicant: Universidade Federal Do Rio De Janeiro
    Inventor: Universidade Federal Do Rio De Janeiro
  • Publication number: 20130145488
    Abstract: The invention provides gold-plated mesoporous silicate bodies comprising pores and at least one agent and methods of using those bodies.
    Type: Application
    Filed: December 6, 2012
    Publication date: June 6, 2013
    Applicant: Iowa State University Research Foundation, Inc.
    Inventors: Kan Wang, Brian G. Trewyn, Susana Martin-Ortigosa, Justin Valenstein
  • Patent number: 8435791
    Abstract: A highly controlled and precise system, device and method for tissue and cellular alteration and treatment below or at surfaces with a laser. The present invention is characterized by ultra low levels of collateral damage as defined by physiologically relevant tests that measure tissue viability. The operation of the present invention is based on spectrally confining the interaction between laser energy and a targeted tissue including an essential element for physiologically relevant tests for monitoring tissue viability.
    Type: Grant
    Filed: March 27, 2003
    Date of Patent: May 7, 2013
    Assignees: Hadasit Medical Research Services and Development Ltd., Yissum Research Development Company of the Hebrew University of Jerusalem
    Inventors: Eithan Galun, Aaron Lewis, Evelyne Zeira, Alexandra Manevitch, Artium Khatchatouriants, Yitzchak Hemo
  • Publication number: 20130109028
    Abstract: The disclosure relates to a method of identification of antiviral molecules that help in efficient viral control and thereby aid in disease management. In particular, the disclosure relates to identification of anti-Tat molecules and hence is directed towards antiviral drug development. The disclosure also relates to Tat-inducible GFP-anti RFP shRNA vector, vector combinations, recombinant cell having instant vectors, methods and kits thereof.
    Type: Application
    Filed: July 11, 2011
    Publication date: May 2, 2013
    Applicant: JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTFIC RESEARCH
    Inventor: Ranga Udaykumar
  • Patent number: 8415160
    Abstract: Disclosed is a transfection method, which includes the steps of: (a) adhering the gene fragments to dry ice particles; (b) adding the dry ice particles into the medium/liquid that contains target cells; and (c) transporting the gene fragments into the target cells via the micro explosion/sublimation of the dry ice particles. In addition, the gene fragments can also adhere first to nanoparticles, which can then adhere to dry ice particles. Subsequently, gene fragments enter cells by micro explosion/sublimation. The present invention can be applied in transgenic research on prokaryotic, eukaryotic, plant and animal cells and in the development of new species in agriculture.
    Type: Grant
    Filed: November 30, 2010
    Date of Patent: April 9, 2013
    Assignee: National Yang Ming University
    Inventors: Fu-Jen Kao, Yung-En Kuo
  • Patent number: 8349364
    Abstract: Particles are provided which are suitable for delivery from a particle-mediated delivery device. The particles are obtained by precipitating a nucleic acid on inert metal carrier particles in the presence of a nucleic acid condensing agent and a metal ion chelating agent. Also described are processes for preparing the particles, and therapeutic methods using the particles including methods of nucleic acid immunization and gene therapy.
    Type: Grant
    Filed: September 29, 2003
    Date of Patent: January 8, 2013
    Assignee: Powderject Research Limited
    Inventors: Chris Robert Lively, Robert DeLong
  • Patent number: 8343766
    Abstract: A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.
    Type: Grant
    Filed: November 28, 2011
    Date of Patent: January 1, 2013
    Assignee: UT-Battle, LLC
    Inventors: Timothy E. McKnight, Anatoli V. Melechko, Michael L. Simpson
  • Patent number: 8323976
    Abstract: Alterations utilizing nanoparticles. Certain embodiments of the invention are methods of delivering a substance to a target using a delivery-aid which includes nanoparticles. Those nanoparticles may be nanocarbon particles. Other embodiments are methods of delivering nanoparticles to a target involving placing a mask between a source of ballistic delivery of nanoparticles and the target. Other embodiments include irradiating a target to cause localized heating of the region of the target in which the nanodiamonds or OLC particles are present. Other embodiments utilize nanoparticles to make cells competent for genetic transformation. This abstract is not to be considered limiting, since other embodiments may deviate from the features described in this abstract.
    Type: Grant
    Filed: January 18, 2006
    Date of Patent: December 4, 2012
    Assignee: International Technology Center
    Inventors: Varvara Grichko, Olga Alexander Shenderova
  • Patent number: 8263406
    Abstract: The present invention is directed to a method of isolating an enriched or purified population of motor neurons from a population of embryonic stem cells. This method involves providing a population of embryonic stem cells and selecting a promoter or enhancer which functions only in the motor neurons selected. A nucleic acid molecule encoding a marker protein under control of the promoter or enhancer is introduced into the induced population of embryonic stem cells. The motor neurons are allowed to express the marker protein and, the cells expressed in the marker protein are separated from the population of embryonic stem cells. The population of embryonic stem cells can be induced to produce a mixed population of cells comprising motor neurons before or after a nucleic acid molecule encoding the marker protein under control of the promoter enhancer is introduced into the population of embryonic stem cells. As a result, an enriched or purified population of motor neurons is isolated.
    Type: Grant
    Filed: June 10, 2004
    Date of Patent: September 11, 2012
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Steven A. Goldman, Neeta Singh Roy, Takahiro Nakano
  • Publication number: 20120135526
    Abstract: Low pressure biolistic barrels and biolistic devices including the same are provided. Aspects of the biolistic barrels include the presence of one or more pressure-reducing elements. Also provided are kits which include the biolistic barrels, as well as methods of delivering a molecule to a target site with the biolistic barrels and devices that include the same. The devices and methods described herein find use in a variety of applications, including in vivo and in vitro high-precision delivery applications.
    Type: Application
    Filed: November 18, 2011
    Publication date: May 31, 2012
    Inventor: Kenneth Greenberg
  • Patent number: 8153432
    Abstract: The present invention relates to automated methods of introducing multiple nucleic acid sequences into one or more target cells.
    Type: Grant
    Filed: April 21, 2009
    Date of Patent: April 10, 2012
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Harris H. Wang, Farren J. Isaacs
  • Patent number: 8133580
    Abstract: The invention relates to an article comprising a coating, which coating comprises at least two layers, of which the inner layer is a primer layer, comprising a supporting polymer network which is composed of a supporting polymer selected from the group consisting of polyethers and polythioethers, including copolymers thereof, the supporting network optionally comprising a functional non-ionic hydrophilic polymer entangled in the supporting polymer network; and the outer layer is a functional layer comprising a functional non-ionic hydrophilic polymer and a polyelectrolyte.
    Type: Grant
    Filed: December 11, 2006
    Date of Patent: March 13, 2012
    Assignee: DSM IP Assets B.V.
    Inventors: Aylvin Jorge Angelo Athanasius Dias, Guido Joseph Elisabeth Hensen, Johannes Wilhelmus Belt, Marnix Rooijmans, Nicolaes Hubertus Maria De Bont, Edwin Peter Kennedy Currie
  • Patent number: 8097277
    Abstract: The present invention provides dynamic charge state cationic polymers that are useful for delivery of anionic molecules. The dynamic charge state cationic polymers are designed to have cationic charge densities that decrease by removal of removable functional groups from the polymers. The present invention also provides interpolyelectrolyte complexes containing the polymers complexed to a polyanion. Methods for using the interpolyelectrolyte complexes to deliver anionic compounds are also provided.
    Type: Grant
    Filed: November 19, 2010
    Date of Patent: January 17, 2012
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David M. Lynn, Adam D. Miller
  • Publication number: 20110293704
    Abstract: The present invention relates to a technology and method of priming of an immune response using invariant chain linked antigen, when these are used to prime a subsequent booster immunization using any suitable vacci.
    Type: Application
    Filed: November 20, 2009
    Publication date: December 1, 2011
    Applicant: University of Copenhagen
    Inventors: Peter Johannes Holst, Allan Randrup Thomsen, Jan Pravsgaard Christensen, Mirjana Grujic
  • Publication number: 20110212073
    Abstract: A process for inhibiting a mammalian cancerous cell or virally infected cell includes providing a Trichomonas vaginalis purine nucleoside phosphorylase enzyme or a tail mutant purine nucleoside phosphorylase enzyme in proximity to the mammalian cancerous cell or the virally infected cell and exposing the enzyme to a purine nucleoside phosphorylase enzyme cleavable substrate to yield a cytotoxic purine analog. The process includes introducing to the cell a vector containing the phosphorylase enzyme, or a DNA sequence coding for the same and delivering to the cell an effective amount of the substrate such as 9-(?-D-arabinofuranosyl)-2-fluoroadenine (F-araA).
    Type: Application
    Filed: August 17, 2009
    Publication date: September 1, 2011
    Applicants: THE UAB RESEARCH FOUNDATION
    Inventors: William B. Parker, Eric J. Sorscher
  • Patent number: 7981446
    Abstract: The present invention relates to methods of delivering nucleic acids into cells using a nucleic acid binding molecule containing a multimeric or spacer-incorporated protein transduction domain (PTD). The invention also relates to novel compositions that contain a nucleic acid complexed or conjugated with a nucleic acid binding molecule. The nucleic acid binding molecule may contain a multimeric or spacer-incorporated PTD, and may further contain a nucleic acid binding region. The nucleic acid complexes or conjugations of the present invention may be employed to inhibit expression of a target gene, and/or determine the function of a target gene.
    Type: Grant
    Filed: November 24, 2008
    Date of Patent: July 19, 2011
    Assignee: ForHumanTech. Co., Ltd.
    Inventors: Sang-Kyou Lee, Seung-Kyou Lee, Ki-Doo Choi
  • Patent number: 7955827
    Abstract: Electroporation is performed in a controlled manner in either individual or multiple biological cells or biological tissue by monitoring the electrical impedance, defined herein as the ratio of current to voltage in the electroporation cell. The impedance detects the onset of electroporation in the biological cell(s), and this information is used to control the intensity and duration of the voltage to assure that electroporation has occurred without destroying the cell(s). This is applicable to electroporation in general. In addition, a particular method and apparatus are disclosed in which electroporation and/or mass transfer across a cell membrane are accomplished by securing a cell across an opening in a barrier between two chambers such that the cell closes the opening. The barrier is either electrically insulating, impermeable to the solute, or both, depending on whether pore formation, diffusive transport of the solute across the membrane, or both are sought.
    Type: Grant
    Filed: April 9, 2010
    Date of Patent: June 7, 2011
    Assignee: The Regents of the University of California
    Inventors: Boris Rubinsky, Yong Huang
  • Patent number: 7927874
    Abstract: There is provided a method by which multiple types of substances desired to be transferred into cells can be continuously transferred into multiple types of cells by a convenient procedure, a cell in which the substance desired to be transferred into cells has been taken up by this method, and an apparatus for transferring a substance into cells by this method. The foregoing objects can be achieved by electrospraying cells with a liquid free from the substance to be transferred into cells while the cells are kept in contact with the substance to be transferred into cells, or first electrospraying cells with a liquid free from the substance to be transferred into cells and then bringing the cells into contact with the substance to be transferred into cells.
    Type: Grant
    Filed: May 16, 2007
    Date of Patent: April 19, 2011
    Assignees: Mitsubishi Gas Chemical Company, Inc., Saitama University
    Inventors: Kazuto Ikemoto, Yusuke Okubo, Kanako Koike, Sayaka Aizawa, Ichiro Sakata, Takafumi Skai
  • Publication number: 20110086090
    Abstract: The present invention refers to the construction of cloning vectors containing the max gene. Especially, the present invention refers to the introduction of cloning vectors containing the max gene in cells using transport vectors. In addition, the presence of cloning vectors containing the max gene in cells allows the differential expression of the max gene in the same cells. In addition, the present invention refers to a method of gene therapy in which the differential expression of the max gene has cytoprotective activity, especially neuroprotective activity, and is capable of application to medical and veterinary therapeutics of neurodegenerative conditions.
    Type: Application
    Filed: April 3, 2009
    Publication date: April 14, 2011
    Applicant: UNIVERSIDADE FEDERAL DO RIO DE JANEIRO
    Inventors: Rafael Linden, Hilda Petrs Silva, Luciana Barreto Chiarini
  • Patent number: 7892836
    Abstract: The capillary gun for delivery of ballistic particles to a target includes an inner capillary tube disposed concentrically within an outer capillary tube with the input end of the inner tube connected to a channel through which a continuous flow of high speed helium gas carrying ballistic particles is introduced. The outer capillary tube, which is connected to a vacuum source, has an outlet end that extends slightly beyond the end of the inner tube. A cap placed over the output end of the outer tube has an opening at its center through which the particles exit the device. The vacuum source applies continuous suction to the space between the outer tube and the inner tube, drawing the gas from the output end of the inner tube while the inertia of the accelerated particles causes them to continue in the axial direction through the exit opening for delivery to the target. Multiple particle injectors provide for the concurrent injection of different materials without disruption of the gas flow.
    Type: Grant
    Filed: February 13, 2006
    Date of Patent: February 22, 2011
    Assignee: The Regents of the University of California
    Inventors: Alexander Groisman, Claire Simonnet, Dmitry Rinberg
  • Patent number: 7888102
    Abstract: The invention relates to the production of coronaviruses. In particular, the invention relates to methods for producing SARS-CoV by using cells expressing a functional SARS-CoV receptor.
    Type: Grant
    Filed: July 21, 2006
    Date of Patent: February 15, 2011
    Assignee: Crucell Holland B.V.
    Inventors: Edward Norbert Van Den Brink, Jan Henrik Ter Meulen
  • Patent number: 7883720
    Abstract: The present invention provides dynamic charge state cationic polymers that are useful for delivery of anionic molecules. The dynamic charge state cationic polymers are designed to have cationic charge densities that decrease by removal of removable functional groups from the polymers. The present invention also provides interpolyelectrolyte complexes containing the polymers complexed to a polyanion. Methods for using the interpolyelectrolyte complexes to deliver anionic compounds are also provided.
    Type: Grant
    Filed: July 7, 2004
    Date of Patent: February 8, 2011
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David M. Lynn, Adam D. Miller
  • Patent number: 7718409
    Abstract: Electroporation is performed in a controlled manner in either individual or multiple biological cells or biological tissue by monitoring the electrical impedance, defined herein as the ratio of current to voltage in the electroporation cell. The impedance detects the onset of electroporation in the biological cell(s), and this information is used to control the intensity and duration of the voltage to assure that electroporation has occurred without destroying the cell(s). This is applicable to electroporation in general. In addition, a particular method and apparatus are disclosed in which electroporation and/or mass transfer across a cell membrane are accomplished by securing a cell across an opening in a barrier between two chambers such that the cell closes the opening. The barrier is either electrically insulating, impermeable to the solute, or both, depending on whether pore formation, diffusive transport of the solute across the membrane, or both are sought.
    Type: Grant
    Filed: January 25, 2006
    Date of Patent: May 18, 2010
    Assignee: The Regents of the University of California
    Inventors: Boris Rubinsky, Yong Huang
  • Patent number: 7699801
    Abstract: Reagents useful in nucleic acid immunization techniques are described. More particularly, adjuvanted genetic vaccine compositions are described, as are methods of using those compositions for inducing an enhanced immune response against a selected antigen.
    Type: Grant
    Filed: May 22, 2007
    Date of Patent: April 20, 2010
    Assignee: Powderject Vaccines, Inc.
    Inventors: Joel R. Haynes, Georg Widera, James T. Fuller, Timothy Shipley, Deborah Fuller, Mary Wu
  • Publication number: 20100041054
    Abstract: Methods and composition of induction of pluripotent stem cells are disclosed. For example, in certain aspects methods for generating induced pluripotent stem cells using reporter genes are described. Furthermore, the invention provides novel reprogramming vectors employing reporter genes.
    Type: Application
    Filed: August 11, 2009
    Publication date: February 18, 2010
    Inventor: Amanda Mack
  • Publication number: 20100029750
    Abstract: The present invention relates to a novel crosslinked polyethylenimine (PEI) nanoparticle based nucleic acid transfection agent wherein the crosslinker is having carbon chain in the range of C2 to C8, ranging between 3.27-19.8%, having the size of nanoparticle ranging between 20-600 nm and zeta potential ranging from +5 to 50 mV.
    Type: Application
    Filed: July 29, 2008
    Publication date: February 4, 2010
    Inventors: Kailash Chand GUPTA, Pradeep KUMAR, Archana SWAMI, Atul PATHAK
  • Publication number: 20090233367
    Abstract: There is provided a method by which multiple types of substances desired to be transferred into cells can be continuously transferred into multiple types of cells by a convenient procedure, a cell in which the substance desired to be transferred into cells has been taken up by this method, and an apparatus for transferring a substance into cells by this method. The foregoing objects can be achieved by electrospraying cells with a liquid free from the substance to be transferred into cells while the cells are kept in contact with the substance to be transferred into cells, or first electrospraying cells with a liquid free from the substance to be transferred into cells and then bringing the cells into contact with the substance to be transferred into cells.
    Type: Application
    Filed: May 16, 2007
    Publication date: September 17, 2009
    Applicants: MITSUBISHI GAS CHEMICAL CO., INC., SAITAMA UNIVERSITY
    Inventors: Kazuto Ikemoto, Yusuke Okubo, Kanako Koike, Sayaka Aizawa, Ichiro Sakata, Takafumi Sakai
  • Publication number: 20090186805
    Abstract: Disclosed are methods and compositions to genetically modify substantially intact cells having cosmetic function to enhance the cosmetic appearance in mammals so as to enhance and/or maintain a biochemical and/or physiological process that has a positive effect on cosmetic appearance. The methods and compositions may provide cosmetic benefits such as reduced skin sagging, increased skin thickness, reduced wrinkles, increased skin thickness and collagen content, increased skin tone and elasticity, increased skin hydration, and improved skin texture and color.
    Type: Application
    Filed: January 2, 2009
    Publication date: July 23, 2009
    Inventors: Aaron Thomas Tabor, John Watson Harmon
  • Patent number: 7544670
    Abstract: The present invention provides methods and compositions useful in localized transfer of genetic material or proteins. Moreover, the present invention provides methods and compositions for improving and/or controlling wound healing by applying a wound care device comprising HoxD3 and/or HoxA3 and/or HoxB3. In addition, the present invention provides methods and compositions for improved wound healing in subjects having impaired healing capabilities, such as diabetic and aged subjects.
    Type: Grant
    Filed: December 29, 2006
    Date of Patent: June 9, 2009
    Assignee: The Regents of the University of California
    Inventors: Nancy Boudreau, David M. Young, Cornelia Myers
  • Publication number: 20090130762
    Abstract: The invention provides a method of activating hepatitis C virus (HCV)-specific T cells, including CD4+ and CD8+ T cells. HCV-specific T cells are activated using fusion proteins comprising HCV NS3, NS4, NS5a, and NS5b polypeptides, polynucleotides encoding such fusion proteins, or polypeptide or polynucleotide compositions containing the individual components of these fusions. The method can be used in model systems to develop HCV-specific immunogenic compositions, as well as to immunize a mammal against HCV.
    Type: Application
    Filed: October 17, 2007
    Publication date: May 21, 2009
    Inventors: Xavier Paliard, Michael Houghton, Mark Selby
  • Publication number: 20090098653
    Abstract: The invention provides transgenomic mitochondria, transmitochondrial cells and organisms, and the materials and methods for making such mitochondria, cells, and organisms.
    Type: Application
    Filed: April 15, 2005
    Publication date: April 16, 2009
    Inventors: Michael D. Koob, Young G. Yoon
  • Publication number: 20090068745
    Abstract: The invention relates to compositions and methods for reverse gene therapy, wherein a gene therapy vector encoding a gene product (e.g. a protein) which is usually only expressed in cells of an abnormal tissue is delivered to a cell of an animal afflicted with a disease or disorder to alleviate the disease or disorder. In one embodiment, a plasmid vector encoding HERG (A561V) protein is delivered to a cell of an animal afflicted with re-entrant atrial flutter-mediated cardiac arrhythmia.
    Type: Application
    Filed: October 15, 2007
    Publication date: March 12, 2009
    Inventors: Robert J. Levy, Denise Y. Burton