Plasmid Or Episome Contains At Least Part Of A Gene Encoding A Toxin Or Encoding For Virulence Or Pathogenicity Patents (Class 435/480)
  • Patent number: 9018014
    Abstract: The invention provides vectors and methods for directional cloning.
    Type: Grant
    Filed: October 17, 2011
    Date of Patent: April 28, 2015
    Assignee: Promega Corporation
    Inventors: Michael R. Slater, Keith V. Wood, James Robert Hartnett
  • Publication number: 20150093473
    Abstract: The present invention provides methods and compositions related to modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some preferred embodiments, the present invention provides compositions and methods for the use of one or more cas genes or proteins for modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some embodiments, the present invention provides methods and compositions that find use in the development and use of strain combinations and starter culture rotations. In additional embodiments, the present invention provides methods for labelling and/or identifying bacteria. In some preferred embodiments, the present invention provides methods for the use of CRISPR loci to determine the potential virulence of a phage against a cell and the use of CRISPR-cas to modulate the genetic sequence of a phage for increased virulence level.
    Type: Application
    Filed: December 15, 2014
    Publication date: April 2, 2015
    Inventors: Rodolphe Barrangou, Christophe Fremaux, Phillippe Horvath, Dennis Romero, Patrick Boyaval
  • Patent number: 8877502
    Abstract: The present invention relates to plasmid curing, and particularly to efficient and stress-free methods for displacing resident or endogenous plasmids from a host cell, such as a bacterium. The invention extends to method of displacing a plasmid comprising a post-segregational killing system from a host cell, the method comprising introducing a recombinant nucleic acid molecule into a host cell harboring a plasmid comprising a post-segregational killing (PSK) system, characterized in that the recombinant nucleic acid molecule is adapted to neutralize the toxic effects of the plasmid's post-segregational killing system, and wherein the nucleic acid molecule is also adapted to outcompete or inhibit replication of the plasmid. The invention further extends to recombinant nucleic acid molecules that can be used in this method, as well as further uses of the methods and nucleic acid molecules of the invention.
    Type: Grant
    Filed: May 9, 2007
    Date of Patent: November 4, 2014
    Assignee: The University of Birmingham
    Inventor: Christopher Morton Thomas
  • Publication number: 20140106458
    Abstract: The present invention provides novel C. botulinum conjugatively transmissible plasmids and methods of use thereof. Specifically, described herein are novel, conjugatively transmissible clostridial plasmids which are capable of being transferred among and between clostridial species. The novel plasmids of the present invention therefore permits the delivery of heterologous clostridial genes into a clostridial host, such as C. botulinum, and the expression of genes of interest in that host, including clostridial toxins and the nontoxigenic components of the toxin complex, toxin fragments, or antigenic portions thereof, in a way both that ensures abundant expression and facilitates purification. Furthermore, toxins with altered structures, chimeric, hybrid toxins, and other toxin derivatives valuable in medicine could be synthesized in this system.
    Type: Application
    Filed: December 4, 2013
    Publication date: April 17, 2014
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Eric A. Johnson, Kristin M. Marshall, Marite Bradshaw
  • Patent number: 8318497
    Abstract: The present invention is related to a reversible, parallel and/or multitask cloning method and kit, which improve the cloning of (preferably multiple) genetic element(s) in a nucleic acid construct such as vector or in chromosome of a cell and the rapid and efficient selection of said construct with a correct integration of said genetic element(s) either in vitro or in vivo.
    Type: Grant
    Filed: September 3, 2003
    Date of Patent: November 27, 2012
    Assignee: Universite Libre de Bruxelles
    Inventors: C├ędric Szpirer, Michel C. Milinkovitch, Philippe Gabant
  • Publication number: 20110171734
    Abstract: The present invention provides novel C. botulinum conjugatively transmissible plasmids and methods of use thereof. Specifically, described herein are novel, conjugatively transmissible clostridial plasmids which are capable of being transferred among and between clostridial species. The novel plasmids of the present invention therefore permits the delivery of heterologous clostridial genes into a clostridial host, such as C. botulinum, and the expression of genes of interest in that host, including clostridial toxins and the nontoxigenic components of the toxin complex, toxin fragments, or antigenic portions thereof, in a way both that ensures abundant expression and facilitates purification. Furthermore, toxins with altered structures, chimeric, hybrid toxins, and other toxin derivatives valuable in medicine could be synthesized in this system.
    Type: Application
    Filed: October 15, 2010
    Publication date: July 14, 2011
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Eric A. Johnson, Kristin M. Marshall, Marite Bradshaw
  • Publication number: 20100129916
    Abstract: Compositions and methods are provided for expression of a toxic protein in a host cell preferably a bacterial host cell where at least one T7 RNA polymerase gene Is contained on the host cell chromosome and one or more genes encoding a T7 RNA polymerase inhibitor is located on an F? plasmid or on the chromosome.
    Type: Application
    Filed: December 3, 2007
    Publication date: May 27, 2010
    Applicant: NEW ENGLAND BIOLABS ,INC.
    Inventors: James C. Samuelson, Theodore B. Davis, Elisabeth A. Raleigh, Maurice W. Southworth
  • Patent number: 7611883
    Abstract: The invention relates to a system for stable maintenance of a plasmid, to host cells for use in this system and to methods of using the system to obtain a plasmid useful in medical applications. In particular, the invention provides transformed host cell containing: i) a chromosomal gene which inhibits cell growth; and ii) a plasmid encoding an antisense sequence, wherein the antisense sequence encoded by the plasmid inhibits the action of the chromosomal gene, thereby permitting cell growth and a method for stable maintenance of a plasmid in a host cell in vivo.
    Type: Grant
    Filed: November 22, 2004
    Date of Patent: November 3, 2009
    Assignee: Cobra Biologics Limited
    Inventor: Rocky Marc Cranenburgh
  • Patent number: 7176029
    Abstract: A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.
    Type: Grant
    Filed: November 19, 2004
    Date of Patent: February 13, 2007
    Assignee: Universite Libre de Bruxelles
    Inventors: Philippe Bernard, Philippe Gabant
  • Patent number: 7125720
    Abstract: The present invention relates generally to a Plasmid Maintenance System for the stabilization of expression plasmids encoding foreign antigens, and methods for making and using the Plasmid Maintenance System. The invention optimizes the maintenance of expression plasmids at two independent levels by: (1) removing sole dependence on balanced lethal maintenance functions; and (2) incorporating at least one plasmid partition function to prevent random segregation of expression plasmids, thereby enhancing their inheritance and stability. The Plasmid Maintenance System may be employed within a plasmid which has been recombinantly engineered to express a variety of expression products.
    Type: Grant
    Filed: September 19, 2005
    Date of Patent: October 24, 2006
    Assignee: University of Maryland, Baltimore
    Inventor: James E. Galen
  • Patent number: 6977176
    Abstract: The present invention relates generally to a Plasmid Maintenance System for the stabilization of expression plasmids encoding foreign antigens, and methods for making and using the Plasmid Maintenance System. The invention optimizes the maintenance of expression plasmids at two independent levels by: (1) removing sole dependence on balanced lethal maintenance functions; and (2) incorporating at least one plasmid partition function to prevent random segregation of expression plasmids, thereby enhancing their inheritance and stability. The Plasmid Maintenance System may be employed within a plasmid which has been recombinantly engineered to express a variety of expression products.
    Type: Grant
    Filed: January 5, 2004
    Date of Patent: December 20, 2005
    Assignee: University of Maryland
    Inventor: James E. Galen
  • Patent number: 6855513
    Abstract: The invention provides methods for identifying a modulator of quorum sensing signaling in bacteria, and for identifying a quorum sensing controlled gene in bacteria. In addition, the invention provides quorum sensing controlled genetic loci in Pseudomas aeruginosa. Novel indicator strains and vectors for engineering the strains for use in the method of the invention are also provided.
    Type: Grant
    Filed: September 1, 2000
    Date of Patent: February 15, 2005
    Assignees: University of Iowa Research Foundation, Vertex Pharmaceuticals (San Diego) LLC
    Inventors: Marvin Whiteley, Kimberly M. Lee, E. Peter Greenberg, Ute Muh
  • Patent number: 6682729
    Abstract: A method for introducing and expressing genes in animal cells is disclosed comprising infecting the animal cells with live invasive bacteria, wherein the bacteria contain a eukaryotic expression cassette encoding the gene. The gene may encode, e.g., a vaccine antigen, a therapeutic agent, an immunoregulatory agent or an anti-sense RNA or a catalytic RNA.
    Type: Grant
    Filed: October 19, 1999
    Date of Patent: January 27, 2004
    Assignee: University of Maryland, Baltimore
    Inventors: Robert J. Powell, George K. Lewis, David M. Hone
  • Publication number: 20030192078
    Abstract: A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, B.t.k. HD-73, B.t. tenebrionis, B.t. entomocidus, 2 protein of B.t.k. HD-1, and the coat protein of potato leaf roll virus.
    Type: Application
    Filed: March 19, 2002
    Publication date: October 9, 2003
    Inventors: David A. Fischhoff, Frederick J. Perlak
  • Patent number: 6617163
    Abstract: The invention concerns a new tool for efficient mutagenesis enabling the generation of a collection of mutants in fungi by random insertion of a characterized Fusarium oxysporum Impala transposon in the genome of said fungi. The invention also concerns the resulting mutants.
    Type: Grant
    Filed: December 26, 2001
    Date of Patent: September 9, 2003
    Assignee: Aventis Cropscience S.A.
    Inventors: Marie-Claire Grosjean-Cournoyer, Francois Villalba, Marc-Henri Lebrun, Marie-Josee Daboussi
  • Patent number: 6605701
    Abstract: Disclosed and claimed are novel toxins and genes obtainable from Bacillus laterosporus isolates disclosed herein. In preferred embodiments, the subject genes and toxins are used to control Western corn rootworm.
    Type: Grant
    Filed: September 28, 2001
    Date of Patent: August 12, 2003
    Assignee: Mycogen Corporation
    Inventors: H. Ernest Schnepf, Kenneth E. Narva, Brian A. Stockhoff, Stacey Finstad Lee, Mikki Walz, Blake Sturgis
  • Patent number: 6602712
    Abstract: A enterotoxin-deficient mutant of a member strain of the Bacillus cereus group does not produce HBL enterotoxin, which has been regarded as a human pathogen found in member strains. An enterotoxin-deficient mutant is suitable for use as a biocontrol agent. Methods for making the mutant and for using the mutant are described.
    Type: Grant
    Filed: April 7, 1999
    Date of Patent: August 5, 2003
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Jo Handelsman, Amy K. Klimowicz
  • Patent number: 6593141
    Abstract: This invention relates to an isolated nucleic acid fragment encoding scorpion toxins that are K-channel modifiers. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the K-channel modifier, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the K-channel modifier in a transformed host cell.
    Type: Grant
    Filed: June 1, 2001
    Date of Patent: July 15, 2003
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Rafael Herman, James F. Wong, Albert L. Lu, James K. Presnail, Jian-Ming Lee
  • Patent number: 6548246
    Abstract: The present invention relates to a class of microbial coding sequences the transcription or cotranscription of which is specifically induced during microbial infection of a host. These particular coding sequences or defined regions thereof may be used as probes to identify and isolate microbial virulence genes. The products of these virulence genes will provide potential targets for the development of vaccines or antimicrobial agents.
    Type: Grant
    Filed: November 17, 1998
    Date of Patent: April 15, 2003
    Assignee: The Regents of the University of California
    Inventors: Michael J. Mahan, Christopher P. Conner, Douglas M. Heithoff
  • Patent number: 6521458
    Abstract: The present invention provides methods for eliminating plants containing non-T-DNA sequences derived from a T-DNA vector. More specifically, the present invention provides a method for killing plant cells that receive non-T-DNA sequences based on incorporation of a lethal polynucleotide sequence into the non-T-DNA portion of the vector.
    Type: Grant
    Filed: April 30, 1999
    Date of Patent: February 18, 2003
    Assignee: DNA Plant Technology Corporation
    Inventors: Neal Gutterson, William G. Hanson
  • Patent number: 6503500
    Abstract: The invention provides a biocontrol agent against larvae of mosquitoes and blackflies comprising transgenic Anabaena PCC 7120 carrying a synergistic combination of the endotoxin genes CryIVA and CryIVD of Bacillus thuringiensis subsp. israelensis.
    Type: Grant
    Filed: November 13, 1998
    Date of Patent: January 7, 2003
    Assignee: Ben Gurion University of the Negev Research and Development Authority
    Inventors: Arieh Zaritsky, Sammy Boussiba, Eitan Ben-Dov
  • Patent number: 6297369
    Abstract: Disclosed and claimed are novel toxins and genes obtainable from Bacillus laterosporus isolates disclosed herein. In preferred embodiments, the subject genes and toxins are used to control Western corn rootworm.
    Type: Grant
    Filed: August 10, 1999
    Date of Patent: October 2, 2001
    Assignee: Mycogen Corporation
    Inventors: H. Ernest Schnepf, Kenneth E. Narva, Brian A. Stockhoff, Stacey Finstad Lee, Mikki Walz, Blake Sturgis
  • Patent number: 6267966
    Abstract: Methods of preparing recombinant Bacillus anthracis protective antigen or a variant or fragment thereof for use in vaccines is disclosed. The protein is expressed in a recombinant microorganism which comprises a sequence which encodes PA or said variant or fragment thereof wherein either (i) a gene of the microorganism which encodes a catabolic repressor protein and/or AbrB is inactivated, and/or (ii) wherein a region of the PA sequence which can act as a catabolic repressor binding site and/or an AbrB binding site is inactivated. Useful quantities of protein are obtainable from these organisms.
    Type: Grant
    Filed: February 25, 1999
    Date of Patent: July 31, 2001
    Assignee: The Secretary of State for Defence
    Inventor: Leslie W J Baillie
  • Patent number: 6252138
    Abstract: New pathogen-induced promoters are provided, particularly nematode-induced promoters, which are characterized by their selective induction of expression in the vicinity of the pathogen infection sites, such as the fixed feeding cells induced by infection of the plant by nematodes. Further provided are chimeric genes comprising these promoters as regulatory elements, as well as transgenic plants, comprising those chimeric genes, which are less susceptible to pathogen infections.
    Type: Grant
    Filed: July 20, 1999
    Date of Patent: June 26, 2001
    Assignee: Plant Genetic Systems, N.V.
    Inventors: Mansour Karimi, Nathalie Barthels, Godelieve Gheysen
  • Patent number: 6239329
    Abstract: A novel tissue-specific promoter is provided which has been isolated from the upstream non-coding region of a plant UFO gene. This promoter, operably associated with a nucleic acid sequence expressing a product of interest, initiates and regulates the transcription of such sequences in a shoot meristem-specific tissue.
    Type: Grant
    Filed: March 9, 1999
    Date of Patent: May 29, 2001
    Assignee: The Salk Institute for Biological Studies
    Inventors: Detlef Weigel, Ilha Lee
  • Patent number: 6180407
    Abstract: A cloning and/or sequencing vector enables recombinant clones to be selected directly. The vector encodes a fusion protein which includes a protein poison.
    Type: Grant
    Filed: January 4, 1998
    Date of Patent: January 30, 2001
    Assignee: Universite Libre de Bruxelles
    Inventors: Philippe Bernard, Philippe Gabant
  • Patent number: 6150170
    Abstract: A method for introducing and expressing genes in animal cells is disclosed comprising infecting said animal cells with live invasive bacteria, wherein said bacteria contain a eukaryotic expression cassette encoding said gene. The gene may encode, e.g., a vaccine antigen, an therapeutic agent, an immunoregulatory agent or a anti-sense RNA or a catalytic RNA.
    Type: Grant
    Filed: July 30, 1998
    Date of Patent: November 21, 2000
    Assignee: University of Maryland at Baltimore
    Inventors: Robert J. Powell, George K. Lewis, David M. Hone
  • Patent number: 6090562
    Abstract: Cloning systems useful for the isolation of recombinant nucleic acid are disclosed in which the recombination of cloning-system nucleic acid and foreign nucleic acid is linked to the expression of a moiety on the surface of a host organism, the moiety being a first member of a binding pair. When recombination occurs between the nucleic acid and the foreign nucleic acid, the moiety is expressed on the surface of the host organism. The isolation of recombinant nucleic acid is then performed by attaching a second member of the binding pair to a solid support and contacting the host organism with the support. When the first member of the binding pair is expressed on the surface of the host organism, the host organism binds to the second member of the binding pair attached to the solid support, thereby selectively isolating those organisms.
    Type: Grant
    Filed: February 2, 1999
    Date of Patent: July 18, 2000
    Assignee: The Perkin-Elmer Corporation
    Inventors: John A. Bridgham, John Brandis, John Leong, Paul D. Hoeprich, Jr., Charles L. Sloan, Roger A. O'Neill
  • Patent number: 5965381
    Abstract: The present invention relates to recombinant Yersinia and the use thereof for delivery of proteins into eukaryotic cells, including related compositions and methods of treatment and related assays.
    Type: Grant
    Filed: March 6, 1998
    Date of Patent: October 12, 1999
    Assignees: Ludwig Institute for Cancer Research, Universite Catholique de Louvain
    Inventors: Pierre B. van der Bruggen, Guy R. Cornelis, Anne M. Boland, Thierry R. Boon-Falleur
  • Patent number: 5928891
    Abstract: The invention relates to a vector for transforming microorganisms capable of undergoing sporulation and which contains itself a heterologous insert comprising a DNA sequence coding for at least part of crystal protein, particularly that of B. thuringensis. It also concerns the polypeptides expressed by said microorganisms and having insecticidal properties similar to those of crystal protein, the microorganisms themselves, as transformed by this vector and insecticidal composition including either said polypeptide or the microorganism itself.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: July 27, 1999
    Assignees: Institut Pasteur, Centre National de La Recherche Scientifique
    Inventors: Andre Klier, Georges Rapoport, Raymond Dedonder
  • Patent number: 5922603
    Abstract: Strains of Trichoderma spp. that are improved in their capacity as biological control agents for phytopathogenic fungi and nematodes are obtained that are able to over-produce a proteinase, with the aid of a transformation method which involves introducing the gene prb1 of Trichoderma that codes for the proteinase PrB1 under the control of adequate means of regulating expression, producing multiple copies in a stable manner; this ensures that the control achieved of the disease that is caused by pathogenic fungi or nematodes is better in the transgenic strain than in the uncultivated strain that is used as a receptor of the genetic information.
    Type: Grant
    Filed: July 25, 1997
    Date of Patent: July 13, 1999
    Inventors: Alfredo H. Herrera-Estrella, Alberto Flores-Martinez, Ilan Chet
  • Patent number: 5882653
    Abstract: Avirulent Vibrio cholerae strains of O1 (CVD111) and non-O1 (CVD112 and CVD112RM) serogroups having the DNA of the cholera toxin core and the RS1 sequences of the cholera toxin locus deleted, and further having a DNA encoding a resistance to mercury, and a DNA encoding the cholera toxin B subunit, or a part thereof sufficient to confer immunogenicity, re-inserted in the chromosome. Methods of making the avirulent V. cholerae O1 and non-O1 strains of the invention, and cholera vaccines using these strains.
    Type: Grant
    Filed: July 29, 1996
    Date of Patent: March 16, 1999
    Assignee: The University of Maryland System
    Inventors: James B. Kaper, Myron M. Levine
  • Patent number: 5877159
    Abstract: A method for introducing and expressing genes in animal cells is disclosed comprising infecting said animal cells with live invasive bacteria, wherein said bacteria contain a eukaryotic expression cassette encoding said gene. The gene may encode, e.g., a vaccine antigen, an therapeutic agent, an immunoregulatory agent or a anti-sense RNA or a catalytic RNA.
    Type: Grant
    Filed: May 3, 1995
    Date of Patent: March 2, 1999
    Assignee: University of Maryland at Baltimore
    Inventors: Robert J. Powell, George K. Lewis, David M. Hone