Microorganism Of The Genus Brevibacterium Or The Genus Corynebacterium Is A Host For The Plasmid Or Episome Patents (Class 435/487)
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Patent number: 8741623Abstract: Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.Type: GrantFiled: March 23, 2007Date of Patent: June 3, 2014Assignee: BASF SEInventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
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Publication number: 20130102037Abstract: Disclosed is an L-lysine-producing microorganism having gluconate kinase activity weakened in comparison to the endogenous activity thereof, and methods provided for preparing the microorganism and for producing L-lysine using the same.Type: ApplicationFiled: July 15, 2011Publication date: April 25, 2013Applicant: CJ CHEILJEDANG CORPORATIONInventors: Sang Jo Lim, Jun Ok Moon, Hyung Joon Kim, Jae Woo Jang, Jae Yong Cho
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Patent number: 7863435Abstract: The present invention relates to a preparation method of an L-threonine producing strain by utilizing a novel L-threonine importer identified from Corynebacterium glutamicum. The method can be advantageously used for the production of L-threonine by increasing the fermentation concentration of Lthreonine and the yield per unit thereof.Type: GrantFiled: November 23, 2004Date of Patent: January 4, 2011Assignee: CJ Cheiljedang Corp.Inventors: Young-Hoon Park, Sang-Jo Lim, Seong-Jun Kim
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Patent number: 7838278Abstract: Nucleotide and protein sequences that encode enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: GrantFiled: March 23, 2009Date of Patent: November 23, 2010Assignee: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, P. John Rayapati, Nyerhovwo John Tonukari
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Patent number: 7785840Abstract: An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another proteinogenic amino acid, and possesses citrate synthase activity. In addition, a vector comprises the polynucleotide and a bacterium comprises the vector. An isolated polynucleotide comprises a nucleotide sequence comprising, from position 1 to 39, the nucleotide sequence corresponding to position 1 to 39 of SEQ ID NO: 11, from position 40 to 105, a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 12, with each proteinogenic amino acid except L-aspartic acid being present at position 5. A method of producing an L-amino acids is also described.Type: GrantFiled: July 13, 2007Date of Patent: August 31, 2010Assignee: Evonik Degussa GmbHInventors: Brigitte Bathe, Wilfried Claes
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Patent number: 7566563Abstract: Nucleotide sequences and genetic constructs that can be used to regulate genes encoding enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: GrantFiled: August 21, 2008Date of Patent: July 28, 2009Assignee: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, Gyan Rai, P. John Rayapati, Nyerhovwo John Tonukari
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Publication number: 20090004745Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: July 16, 2005Publication date: January 1, 2009Applicant: BASF AktiengesellschaftInventors: Jong-Soo Choi, Weol Kyu Jeong, Il Kwon Kim, Seong Han Lim, Heung-Shick Lee
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Patent number: 7160704Abstract: The present invention discloses a method for producing a target substance using a coryneform bacterium comprising culturing a coryneform bacterium having an ability to produce the target substance in a medium, resulting in accumulation of the target substance in the medium or cells of the bacterium, and collecting the target substance from the medium or the cells of the bacterium. Also disclosed is a coryneform bacterium which is introduced with a methanol dehydrogenase gene and which has enhanced activities of hexulose phosphate synthase and phosphohexuloisomerase, and to which an ability to utilize methanol is imparted or which has enhanced ability to utilize methanol, and the medium contains methanol as a carbon source.Type: GrantFiled: March 4, 2004Date of Patent: January 9, 2007Assignee: Ajinomoto Co., Inc.Inventors: Ryo Takeshita, Hisashi Yasueda
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Patent number: 6586214Abstract: The present invention is directed to isolated polynucleotides coding for phosphoglucose isomerase (pgi) from coryneform bacteria. In addition, the invention includes methods for increasing the metabolic flux through pentose phosphate cycle of bacteria by reducing or eliminating the activity of pgi. These methods may be used to increase the fermentative production of nucleotides, vitamins and amino acids.Type: GrantFiled: September 15, 1999Date of Patent: July 1, 2003Assignee: Degussa AGInventors: L. K. Dunican, Ashling McCormack, Cliona Stapleton, Kevin Burke, Michael O'Donohue, Achim Marx, Bettina Mockel
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Patent number: 6500644Abstract: A method for in vivo production of a library in cells comprising a multitude of mutated genetic elements, wherein an error-prone polymerase is used in each ancestral cell to replicate all or a part of a genetic element independently of the host chromosomal replication machinery. The genetic element comprises i) an origin of replication from which replication is initiated, ii) optionally a genetic marker, e.g. a gene conferring resistance towards an antibiotic, iii) a gene encoding the polypeptide of interest. Also methods for the generation of a DNA sequence encoding a desired variant of a polypeptide of interest, and for the determination of such a DNA sequence are described.Type: GrantFiled: September 26, 2000Date of Patent: December 31, 2002Assignee: Novozymes A/SInventors: Torben Vedel Borchert, Stanislas Dusko Ehrlich
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Patent number: 6204063Abstract: This invention relates to an isolated nucleic acid fragment encoding a glycolysis or respiration protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the glycolysis or respiration protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the glycolysis or respiration protein in a transformed host cell.Type: GrantFiled: March 15, 1999Date of Patent: March 20, 2001Assignee: E. I. du Pont de Nemours and CompanyInventors: Stephen M. Allen, Jian-Ming Lee, Jonathan E. Lightner, Joan T. Odell
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Patent number: 6180373Abstract: A tryptophan producing strain of microorganism is selected from E. coli and Corynebacteria and is tryptophan feedback resistant and serine feedback resistant. The serine feedback resistance is by a mutation in a serA allele, where the mutated serA allele codes for a protein which has a Ki value for serine between 0.1 mM and 50 mM. The tryptophan feedback resistance is by a trpE allele which codes for a protein which has a Ki value for tryptophan between 0.1 mM and 20 mM. A process for preparing this microorganism and a process for using this microorganism are disclosed.Type: GrantFiled: April 12, 1995Date of Patent: January 30, 2001Assignee: Consortium f{umlaut over (u)}r elektrochemische Industrie GmbHInventors: G{umlaut over (u)}nter Wich, Walfred Leinfelder, Keith Backman
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Patent number: 6048694Abstract: A positive selection vector is provided for the transformation and screening of Gram positive bacteria and particularly Bacillus sp. for the presence of foreign DNA. The vector comprises a mutant gene encoding a signal peptide processing mutation. Expression of the mutant gene in a Bacillus host cell which lacks the ability to metabolize sucrose is lethal when cells are grown in the presence of sucrose. Foreign DNA may be inserted into the vector so as to inactivate the mutant gene thereby permitting the cells to grow in the presence of sucrose and allowing for facile selection of transformants.Type: GrantFiled: April 30, 1998Date of Patent: April 11, 2000Assignee: E. I. du Pont de Nemours and CompanyInventors: Michael Gene Bramucci, Vasantha Nagarajan
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Patent number: 5965391Abstract: The invention concerns a DNA fragment located in front of the malate synthase gene of a coryne-form bacterium and isolated from the latter. Any structural gene which codes for a protein can be inserted after this DNA fragment. After transformation of such a construct into a coryne-form bacterium, expression of the structural gene inserted after the DNA fragment is regulated. The invention also concerns a process for synthesizing any protein by culturing a transformed coryne-form bacterium. A bacterium of this type contains in replicable form a DNA fragment isolated from the malate synthase gene of a coryne-form bacterium, and after which the structural gene which codes for the protein to be synthesized is inserted. Since expression of the structural gene which codes for the protein to be synthesized is regulated by the DNA located in front of it, the structural gene is expressed and the desired protein synthesized as soon as a suitable inducing agent is added to the medium.Type: GrantFiled: May 8, 1997Date of Patent: October 12, 1999Assignee: Forschungszentrum Julich GmbHInventors: Dieter Reinscheid, Bernhard Eikmanns, Hermann Sahm