Enzyme Separation Or Purification Patents (Class 435/814)
  • Patent number: 8835145
    Abstract: The invention relates to a thrombolytic enzyme referred to as Thrombinase having a molecular weight of 31,000 to 32,000. Such a thrombolytic enzyme can be used for dissolving blood clots. The process comprises culturing a filtrate of Bacillus sphaericus sero type H5a 5b, removing the cell, subjecting the cell supernatant to filtration, salting out the retentate, subjecting the precipitate to dialysis, reprecipitating the precipitate and then reconstituting in buffer and finally decolorizing, purifying and dialyzing.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: September 16, 2014
    Assignees: National Research Development Corporation, India and Malladi Drugs Pharmaceuticals Ltd.
    Inventors: Subrahamanyam Chivukula Sekar, Sundaramurthy Suresh Babu, Sita Mahadevan
  • Patent number: 8758769
    Abstract: Disclosed is a composition for preventing and treating acetaminophen induced liver injury including the protein extracted from Porphyra yezoensis. The protein(s), separated and purified from hot water extracts of Porphyra yezoensis, having the molecular weight of 14 kDa measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis, has an excellent effect for inhibiting the oxidative injury of liver tissue and cell apoptosis of liver cells induced by acetaminophen.
    Type: Grant
    Filed: August 28, 2007
    Date of Patent: June 24, 2014
    Assignee: Pukyong National University Industry Academic Cooperation Foundation
    Inventors: Taek-Jeong Nam, Mi-Jin Kwon, Hye-Jung Hwang
  • Patent number: 7868145
    Abstract: A magnetic particle and fabrication method thereof. The magnetic particle comprises a polymer core, a magnetic material layer covering the polymer core, and a silicon containing layer covering the magnetic material layer. In addition, the magnetic particle may further comprise a coupling agent on the silicon containing layer, and an active molecule connected to the coupling agent. The magnetic particles provide controllable size, uniform diameter distribution, high magnetization, improved storage stability, and modified surface for targeting biomolecules for biomaterial separation and environmental analysis.
    Type: Grant
    Filed: July 11, 2007
    Date of Patent: January 11, 2011
    Assignee: Industrial Technology Research Institute
    Inventors: Kun Chan Wu, Hui-Ju Cho, Pei-Shin Jiang, Hsiang Yuan Huang, Wen-Hsun Kuo, Chi-Min Chau, Chih Hsien Su, Kun Feng Lee
  • Patent number: 7371830
    Abstract: Magnetic particles are prepared containing a magnetic core coated with a glass layer having a substantially pore-free glass surface or having pores with a diameter of less than 10 nm. The particles are used for separating biological material such as nucleic acids. A preferred process of preparing the particles is by forming a mixture of magnetic cores with a sol formed from an alcohol and a metal alkoxide, spray-drying the mixture to coat the cores with a layer of gelled sol, and heating the coated cores to obtain the magnetic glass particles. Preferably, the particles have an average particle size of less than 100 ?m. The magnetic core may be a composite material containing a mica core and magnetite particles immobilized on the mica core, and the glass layer may contain boron oxide. Magnetic core materials include magnetite (Fe3O4) and Fe2O3.
    Type: Grant
    Filed: January 24, 2005
    Date of Patent: May 13, 2008
    Assignee: Roche Diagnostics GmbH
    Inventors: Jörg Kleiber, Thomas Walter, Herbert Harttig, Christop Lesniak, Martin Mennig, Michael Riedling, Helmut Schmidt
  • Patent number: 7332126
    Abstract: Disclosed is a method for performing the steps of nucleic acid template purification, thermocycling reaction and purification of the products of the thermocycling reaction characterized in that the steps take place sequentially in a microfluidic disc. Also disclosed is a microstructure for fluids comprising at least one inlet opening connected to a first chamber incorporating a means for purifying template nucleic acid which, in turn, is connected to a second chamber incorporating a means for a thermocycling reaction which, in turn, is connected to a third chamber incorporating a means for purifying products of the thermocycling reaction, and a microfluidic disc comprising a plurality of such microstructures.
    Type: Grant
    Filed: January 13, 2005
    Date of Patent: February 19, 2008
    Assignee: GYROS Patent AB
    Inventors: Nigel Eric Tooke, Per X. Andersson
  • Patent number: 7115719
    Abstract: Reagents, methods and kits for denaturation of protein are provided.
    Type: Grant
    Filed: December 15, 2004
    Date of Patent: October 3, 2006
    Assignee: Gentra Systems, Inc.
    Inventor: Kim E. Paulsen
  • Patent number: 7112453
    Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.
    Type: Grant
    Filed: August 5, 2002
    Date of Patent: September 26, 2006
    Assignee: Ciphergen Biosystems, Inc.
    Inventors: T. William Hutchens, Tai-Tung Yip
  • Patent number: 7105339
    Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.
    Type: Grant
    Filed: July 23, 2003
    Date of Patent: September 12, 2006
    Assignee: Ciphergen Biosystems, Inc.
    Inventors: T. William Hutchens, Tai-Tung Yip
  • Patent number: 7070941
    Abstract: The invention provides methods and compositions for azide tagging of biomolecules. In one embodiment of the invention, proteins are tagged by metabolic incorporation of prenylated azido-analog substrates. Examples of such analogs are azido farnesyl diphosphate and azido farnesyl alcohol. The azido moiety in the resulting modified proteins provides an affinity tag, which can be chemoselectively captured by an azide-specific conjugation reaction, such as the Staudinger reaction, using a phosphine capture reagent. When the capture agent is biotinylated, the resulting conjugates can be detected and affinity-purified by streptavidin-linked- HRP and streptavidin-conjugated agarose beads, respectively. The invention allows detection and isolation of proteins with high yield, high specificity, and low contamination without harsh treatment of proteins.
    Type: Grant
    Filed: November 17, 2003
    Date of Patent: July 4, 2006
    Assignee: Board of Regents, The University of Texas System
    Inventors: Yingming Zhao, John R. Falck
  • Patent number: 7063976
    Abstract: A process for preparing beta-fructofuranosidase enzyme and a process for producing fructooligosaccharides, in which the preparation of the enzyme is obtained by cultivating the fungus Aspergillus niger, either wild or mutated, in a preferably semi-solid culture medium, in order to produce an extracellular enzyme, which is submitted to transfructosylation for producing fructooligosaccharides comprising sugars which are formed by one unit of sacrose and by two, three and four units of fructose.
    Type: Grant
    Filed: July 15, 2002
    Date of Patent: June 20, 2006
    Assignee: Usina da Barra S/A—Acucar e Alcool
    Inventors: Yong Kun Park, Gláucia Maria Pastores
  • Patent number: 6982158
    Abstract: A process for purifying a phosphodiesterase 1 (PDE-1) from a cell including heating an extract of a cell formed from a solution including at least one divalent cation, to increase the specific activity of PDE-1 in the extract.
    Type: Grant
    Filed: December 11, 2003
    Date of Patent: January 3, 2006
    Assignee: Protech Research Pty Ltd
    Inventor: Michael Patane
  • Patent number: 6884395
    Abstract: Disclosed is a method for performing the steps of nucleic acid template purification, a thermocycling reaction and purification of the products of the thermocycling reaction characterised in that the steps take place sequentially in a microfluidic disc. Also disclosed is a microstructure for fluids comprising at least one inlet opening connected to a first chamber incorporating a means for purifying template nucleic acid which, in turn, is connected to a second chamber incorporating a means for a thermocycling reaction which, in turn, is connected to a third chamber incorporating a means for purifying products of the thermocycling reaction, and a microfluidic disc comprising a plurality of such microstructures.
    Type: Grant
    Filed: December 20, 2000
    Date of Patent: April 26, 2005
    Assignee: Gyros AB
    Inventors: Nigel Eric Tooke, Per Andersson
  • Patent number: 6884597
    Abstract: A method for simply and conveniently detecting acetyltransferase and deacetylase activities of proteins by executing an acetylation reaction of a peptide substrate with an acetyltransferase, or a deacetylation reaction of an acetylated peptide substrate with a deacetylase, and after the completion of these reactions, detecting the acetyl group bound to the peptide substrate by using an anti-acetylated peptide antibody. This system for detecting acetyltransferase and deacetylase activities using the anti-acetylated peptide antibody enables screening inhibitors or enhancers of acetyltransferase and deacetylase. A system for screening deacetylase inhibitors or acetyltransferase enhancers using cultured cells is also provided.
    Type: Grant
    Filed: July 18, 2000
    Date of Patent: April 26, 2005
    Assignee: Medical & Biological Laboratories, Co., Ltd.
    Inventors: Yoichi Taya, Katsuyuki Tamai, Toshiaki Miyazaki
  • Patent number: 6870047
    Abstract: Magnetic particles are prepared containing a magnetic core coated with a glass layer having a substantially pore-free class surface or having pores with a diameter offices than 10 nm. The particles are used for separating biological material such as nucleic acids. A preferred process of preparing the particles is by forming a mixture of magnetic cores with a sol formed from an alcohol and a metal alkoxide, spray-drying the mixture to coat the cores with a layer of gelled sol, and heating the coated cores to obtain the magnetic glass particles. Preferably, the particles have an average particle size of less than 100 ?m. The magnetic core may be a composite material containing a mica core and magnetite particles immobilized on the mica core, and the glass layer may contain boron oxide. Magnetic core materials include magnetite (Fe3O4) and Fe2O3.
    Type: Grant
    Filed: January 10, 2001
    Date of Patent: March 22, 2005
    Assignee: Roche Diagnostics GmbH
    Inventors: Jörg Kleiber, Thomas Walter, Herbert Harttig, Christoph Lesniak, Martin Mennig, Michael Riedling, Helmut Schmidt
  • Patent number: 6830655
    Abstract: The present invention concerns a method for enzymatic treatment of lignocellulosic materials which contain xylan-polymers, such as cellulose kraft pulps. According to a method of the present kind, at least a part of the hexenuronic acid groups present in the material is selectively removed in order to remove metal ions from the pulp, to change the surface charge thereof, to improve the brightness stability of the pulp and to render the material more suitable for enzymatic treatment.
    Type: Grant
    Filed: February 6, 1997
    Date of Patent: December 14, 2004
    Assignee: Valtion Teknillinen Tutkimuskeskus
    Inventors: Matti Siika-Aho, Johanna Buchert, Tapani Vuorinen, Anita Teleman, Maija Tenkanen, Michael Bailey, Liisa Viikari
  • Patent number: 6825027
    Abstract: The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present invention is also directed to an HAV vaccine composition comprising a preparation consisting of purified mature HAV particles in an amount sufficient to induce a protective immune response in a mammal.
    Type: Grant
    Filed: December 10, 2001
    Date of Patent: November 30, 2004
    Assignee: Baxter Healthcare S.A.
    Inventors: Christa Tauer, Heidi Meyer, Artur Mitterer, Noel Barrett
  • Patent number: 6783929
    Abstract: Provided are affinity support materials having intermediate binding affinity for biological samples. Among the materials provided by the present invention are hydrophilic solid supports composed of hydrophilic ligands coupled to hydrophilic matrixes which are compatible with biological samples, for example, a cell line, a biological fluid such as blood, or a tissue cell lysate. The ligands may include affinity property groups and hydrophilic groups pendent from a backbone, and be configured to at least partially resolve components of a biological sample. Affinity supports in accordance with the present invention may be used in a variety of techniques and apparatuses to achieve improved separations of complex biological samples and thereby enhance the results of biological sample component fractionations, enrichments, purifications, expression product determinations and comparisons, and other biological sample processing techniques.
    Type: Grant
    Filed: November 1, 2000
    Date of Patent: August 31, 2004
    Assignee: Chiron Corporation
    Inventors: Ronald N. Zuckermann, Eric Beausoleil, Matthew Wachowicz, Srinivas Kothakota
  • Patent number: 6762062
    Abstract: The present disclosure relates to a method for determining cholesterol in low density lipoprotein comprising the steps of (a) measuring total cholesterol level in a sample containing at least high density lipoprotein, low density lipoprotein, very low density lipoprotein and chylomicron, and (b) measuring cholesterol levels in the high density lipoprotein, very low density lipoprotein and chylomicron in the sample, wherein the cholesterol level in the low density lipoprotein is determined by subtracting a value obtained in the step (b) from a value obtained in the step (a). The present invention enables concurrent determination of cholesterol level in low density lipoprotein and total cholesterol level, facilitating acquisition of two types of biological information at a time.
    Type: Grant
    Filed: September 24, 2001
    Date of Patent: July 13, 2004
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Motokazu Watanabe, Toshihiko Yoshioka, Shiro Nankai
  • Publication number: 20040069426
    Abstract: The present invention concerns a method for enzymatic treatment of lignocellulosic materials which contain xylan-polymers, such as cellulose kraft pulps. According to a method of the present kind, at least a part of the hexenuronic acid groups present in the material is selectively removed in order to remove metal ions from the pulp, to change the surface charge thereof, to improve the brightness stability of the pulp and to render the material more suitable for enzymatic treatment.
    Type: Application
    Filed: February 6, 1997
    Publication date: April 15, 2004
    Inventors: MATTI SIIKA-AHO, JOHANNA BUCHERT, TAPANI VUORINEN, ANITA TELEMAN, MAIJA TENKANEN, MICHAEL BAILEY, LIISA VIIKARI
  • Patent number: 6706486
    Abstract: The present invention relates to a method for the quantitative release of natural or recombinant proteins, polypeptides (thermostable immunoligands) able to bind to the Fc-part of immunoglobulins (antibodies, in particular of the IgG class and primarily becoming bound outside the paratope) from complexes in various sample matrixes in order to make these released natural or recombinant proteins, polypeptides or peptides quantitatively available in immunochemical assays and to keep them quantitatively available. The method is characterized by mixing the sample with reagent compound that is able to bind non-specifically to immunoglobulins, and thereafter subjecting the sample to a heat treatment step followed by a cooling step.
    Type: Grant
    Filed: February 15, 2001
    Date of Patent: March 16, 2004
    Assignee: Amersham Biosciences AB
    Inventor: Franz Steindl
  • Patent number: 6649354
    Abstract: The present invention involves a method for assaying a substance. The method of the present invention comprises contacting the substance with an assay agent comprising a catalytic agent to associate the substance with the catalytic agent, contacting the resulting associated substance with a label precursor capable of reacting catalytically with the catalytic agent to release the label, and detecting a mass label. The present invention also involves a kit for assaying a substance. The kit of the present invention comprises an assay agent comprising a catalytic agent, and a label precursor capable of reacting catalytically with the catalytic agent to release a mass label.
    Type: Grant
    Filed: May 3, 2000
    Date of Patent: November 18, 2003
    Assignee: Xzillion GmbH & Co.
    Inventors: Gunter Schmidt, Andrew Hugin Thompson
  • Patent number: 6635420
    Abstract: The invention concerns a method for the purification of a target substance from a biological sample by immobilizing the target substance on a solid phase by means of a high affinity binding pair and subsequently eluting it by adding a partner of the binding pair in a free form. In addition reagent kits for carrying out the method are disclosed.
    Type: Grant
    Filed: October 13, 2000
    Date of Patent: October 21, 2003
    Assignee: Roche Diagnostics GmbH
    Inventors: Wolfgang Hosel, Helmut Lenz, Jochen Peter
  • Patent number: 6593118
    Abstract: The present invention provides a crystallization process wherein a starting temperature is selected such that a desirable crystal morphology (e.g., square) is obtained. A temperature shift is then introduced, providing that the shift is not enough to induce further nucleation, where the crystals continue to grow in the desirable fashion, but with different kinetics, e.g., a higher rate of crystallization. As a result, the process gives a crystalline product with desirable morphology at a higher crystallization rate.
    Type: Grant
    Filed: November 2, 2001
    Date of Patent: July 15, 2003
    Assignee: Genencor International, Inc.
    Inventor: Meng H. Heng
  • Patent number: 6582705
    Abstract: The invention relates to an immunogenic composition, characterized in that it comprises an adenyl cyclase-hemolysin (AC-Hly) protein, or an immunogenic portion of this AC-Hly, of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B bronchiseptica, and in that it comprises, in addition, a bacterial extract containing the expression products of the vrg genes of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B bronchiseptica, or a portion of these expression products which is sufficient to induce an immune response in a host to which the extract might be administered.
    Type: Grant
    Filed: January 25, 1996
    Date of Patent: June 24, 2003
    Assignee: Institut Pasteur
    Inventors: Pascale Gueirard, Nicole Guiso
  • Patent number: 6576460
    Abstract: The present invention relates to a filtration-detection device for detecting or quantifying an analyte in a test sample including a filtration device having a first binding material immobilized thereto, wherein the first binding material is capable of binding to a portion of the analyte, and a detection assembly positioned relative to the filtration device to detect or quantify analyte bound to the first binding material. The present invention also relates to methods of using the filtration-detection device.
    Type: Grant
    Filed: October 27, 2000
    Date of Patent: June 10, 2003
    Assignees: Cornell Research Foundation, Inc., Innovative Biotechnologies International, Inc.
    Inventors: Antje J. Baeumner, Richard A. Montagna
  • Patent number: 6569649
    Abstract: Processes for preparing oligosaccharides, polysaccharides, glycolipids, glycoproteins, and other saccharide compositions are provided which involve the enzyme facilitated transfer of a preselected saccharide unit from a donor moiety to an acceptor moiety. In accordance with a preferred embodiment, saccharide compositions having a plurality of saccharide units are prepared by appending the saccharide units in iterative fashion to acceptor moieties which are themselves saccharide compositions prepared in accordance with this invention.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: May 27, 2003
    Assignee: The Trustees of the University of Pennsylvania
    Inventor: Stephen Roth
  • Patent number: 6566134
    Abstract: The utility of soybeans having a composition of greater than 40% of the protein as beta-conglycinin and less than 10% of the protein as glycinin for making highly functional high beta-conglycinin compositions was discovered. The discovered ingredients are useful for mimicking the texturizing properties of casein while also maintaining or improving physiological benefits of soy protein ingredients (e.g., cholesterol and triglyceride lowering properties). The high stability of the high beta-conglycinin compositions against protein—protein aggregation reactions is valuable for creating good tasting beverages and beverage mixes. Cheese with good spreadability, gloss and smoothness was made using an enzyme-modified version of the new ingredient composition. Cheese with good firmness and meltability was also created using a different enzyme-treatment. High beta-conglycinin compositions were found to demonstrate excellent emulsifying and gelling properties in the pH region (5.5-6.
    Type: Grant
    Filed: December 20, 2000
    Date of Patent: May 20, 2003
    Assignee: Monsanto Company
    Inventor: Neal A. Bringe
  • Patent number: 6562605
    Abstract: A method is provided for the extraction of water soluble biomaterials such as enzymes or proteins into carbon dioxide utilizing certain carbon dioxide soluble surfactants. The extraction can be performed on an aqueous solution, a fermentation broth or a fluid. The method includes the process steps of forming a carbon dioxide/surfactant mixture which involves dissolving carbon dioxide soluble surfactant(s) in carbon dioxide. The carbon dioxide can be in a liquid or supercritical form and the surfactant includes tail and head groups that interact with the biomaterials. Further, the mixture is added to the aqueous solution, fermentation broth or liquid under conditions to allow for extraction of the biomaterials. The method further includes depressurizing and/or temperature adjusting to remove the water soluble biomaterials. The surfactants include fluroethers, oligomers of propylene-oxide, siloxanes, etc. The biomaterials include proteins or enzymes. The carbon dioxide is suberitical or supercritical.
    Type: Grant
    Filed: November 12, 1996
    Date of Patent: May 13, 2003
    Assignees: Genencor International, Inc., University of Pittsburgh
    Inventors: Eric J. Beckman, Eliador J. Ghenciu, Nathaniel T. Becker, Landon M. Steele
  • Patent number: 6555391
    Abstract: This invention relates methods for conditioning affinity chromatography resins to decrease leaching of the ligand during purification. The methods involve incubating the resin in a buffered solution of a hydroxyalkylamine compound (e.g., ethanolamine) prior to use of the resin for an affinity purification. The treatment removes unstably bound ligand from the resin.
    Type: Grant
    Filed: August 15, 2000
    Date of Patent: April 29, 2003
    Assignee: Baxter International, Inc.
    Inventors: Susan L. Bernhard, Robert Toso, Van Taiariol
  • Patent number: 6406912
    Abstract: The invention relates to a method for producing optically pure compounds of 3(R)- and 3(S)-hydroxy-1-methyl-4-(2,4,6-trimethoxyphenyl)-1,2,3,6-tetrahydro-pyridine or its carboxylic acid esters by reacting the enantiomer mixtures stereoselectively, using an enzyme.
    Type: Grant
    Filed: September 6, 2000
    Date of Patent: June 18, 2002
    Assignee: Aventis Pharma Deutshland GmbH
    Inventor: Wolfgang Holla
  • Patent number: 6403350
    Abstract: The present invention provides a crystallization process wherein a starting temperature is selected such that a desirable crystal morphology (e.g., square) is obtained. A temperature shift is then introduced, providing that the shift is not enough to induce further nucleation, where the crystals continue to grow in the desirable fashion, but with different kinetics, e.g., a higher rate of crystallization. As a result, the process gives a crystalline product with desirable morphology at a higher crystallization rate. The starting temperature of the process can be between about 4° C. and 20° C. for no more than about 5 hours and the temperature shift of the process can be between about 22° C. and 60° C. for no more than about 20 hours.
    Type: Grant
    Filed: March 3, 2000
    Date of Patent: June 11, 2002
    Assignee: Genencor International, Inc.
    Inventor: Meng H. Heng
  • Patent number: 6391654
    Abstract: A kit for testing male fertility comprises a vessel, a base unit, a liquid supply containing liquid and two filters. The first filter is a sample separation filter which forms a hindrance to transmission of spermatozoa. The second filter of the kit is a spermatozoa detection filter comprising a reagent for identifying spermatozoa. Activation of the kit is prevented until a transport medium, such as the liquid, fills a gap allowing spermatozoa to transmit to a detection zone. The kit may be of one-piece construction and utilizes a thin piece of filter material to separate motile from non-motile spermatozoa.
    Type: Grant
    Filed: September 8, 2000
    Date of Patent: May 21, 2002
    Assignee: Genosis Limited
    Inventor: Paul North Bateman
  • Patent number: 6387377
    Abstract: The invention relates to an immunogenic composition, characterized in that it comprises an adenyl cyclase-hemolysin (AC-Hly) protein, or an immunogenic portion of this AC-Hly, of a strain of Bordetella chosen from B. Tertussis, B. parapertussis or B. bronchiseptica, and in that it comprises, in addition, a bacterial extract containing the expression products of the vrg genes of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, or a portion of these expression products which is sufficient to induce an immune response in a host to which the extract might be administered.
    Type: Grant
    Filed: December 15, 1995
    Date of Patent: May 14, 2002
    Assignee: Institut Pasteur
    Inventors: Pascale Gueirard, Nicole Guiso
  • Patent number: 6383819
    Abstract: The present disclosure relates to a method for determining cholesterol in low density lipoprotein comprising the steps of (a) measuring total cholesterol level in a sample containing at least high density lipoprotein, low density lipoprotein, very low density lipoprotein and chylomicron, and (b) measuring cholesterol levels in the high density lipoprotein, very low density lipoprotein and chylomicron in the sample, wherein the cholesterol level in the low density lipoprotein is determined by subtracting a value obtained in step (b) from a value obtained in step (a). The present invention enables concurrent determination of cholesterol level in low density lipoprotein and total cholesterol level, facilitating acquisition of two types of biological information at a time.
    Type: Grant
    Filed: January 27, 2000
    Date of Patent: May 7, 2002
    Assignee: Matsushita Electric Industrial Co., Ltd.
    Inventors: Motokazu Watanabe, Toshihiko Yoshioka, Shiro Nankai
  • Patent number: 6379915
    Abstract: A dry chemistry dye indicator composition provides improved shelf life, stable color indication end point and capability for a system at near normal pH. The novel dry chemistry dye indication system comprises 3-Methyl-6-(sodium sulfonate)-benzothiazolinone-(2)-hydrazone (MBTH-S). A preferred dye systems are based on the dye couple (MBTH-S) and 8-anilino-1-naphthalenesulfonate (ANS), and the dye couple MBTH-S and N-(3-sulfopropyl)analine. These dye indicator systems are used in conventional blood chemistry test strips and are particularly preferred for indication of glucose in blood.
    Type: Grant
    Filed: March 20, 2000
    Date of Patent: April 30, 2002
    Assignee: Amira Medical
    Inventors: Joel S. Douglas, Karen R. Drexler
  • Patent number: 6379599
    Abstract: The invention relates to a process for the preparation of molecularly imprinted polymers useful for separation of enzymes, which comprises the steps of reacting a complex of enzyme and affinity monomer, a comonomer and a crosslinker, with a polymerization initiator and a polymerization accelerator at ambient temperature and pressure for a period ranging between 2 to 24 hrs, thereby obtaining a crosslinked polymer, crushing the cross linked polymer obtained to fine particles, adding a solvent and extracting imprinted enzyme from the polymer, obtaining the molecularly imprinted polymer, contacting the imprinted polymer with aqueous solution containing imprinted enzyme or a mixture of imprinted enzyme and other enzymes and isolating the enzyme-adsorbed polymer.
    Type: Grant
    Filed: January 10, 2000
    Date of Patent: April 30, 2002
    Assignee: Council of Scientific and Industrial Research
    Inventors: Alankar Arun Vaidya, Bhalchandra Shripad Lele, Mohan Gopalkrishna Kulkarni, Raghunath Anant Mashelkar
  • Patent number: 6352852
    Abstract: A method for the purification of human platelet heparanase using chromatographic techniques is described. The method comprises detergent-aided solubilization of a heparanase-containing fraction followed by purification of the heparanase therefrom by concanavalin A, Zn2+-chelating Sepharose, a Blue A or Reactive Red matrix, octyl agarose and gel filtration chromatography. Heparanase so purified has a molecular mass (Mr) of about 50 kDa and degrades both heparin and heparan sulfates.
    Type: Grant
    Filed: January 24, 2000
    Date of Patent: March 5, 2002
    Assignee: The Australian National University
    Inventors: Craig Geoffrey Freeman, Christopher Richard Parish
  • Patent number: 6316695
    Abstract: The present invention cloned a cDNA clone encoding isopentenyl diphosphate (hereafter “IPP”) isomerase (EC 5.3.3.2) from a cDNA library of Hevea brasiliensis latex. The clone has a continuous open reading frame encoding a peptide of 234 amino acids with a predicted molecular mass of 26.7 kDa. The deduced protein is acidic with an isoelectric point of 4.7 and shows high sequence identity with other IPP isomerases. The recombinant protein expressed in Escherichia coli showed IPP isomerase activity. In vitro rubber biosynthesis assays using washed rubber particle (WRP) deprived of initiating allylic diphosphates were performed with the addition of IPP isomerase in the reaction mixture. Results revealed that the recombinant IPP isomerase is catalytically active in catalyzing the conversion of IPP to DMAPP, a key activation step of the basic five-carbon isoprene unit in rubber biosynthesis. Southern analysis indicated that the IPP isomerase is encoded by two genes in Hevea rubber tree.
    Type: Grant
    Filed: April 22, 1999
    Date of Patent: November 13, 2001
    Assignee: Korea Kumho Petrochemical Co., Ltd.
    Inventors: Kyung-Han Han, Hun-Seung Kang, Soo-Kyung Oh, Dong-Ho Shin, Jae-Mo Yang
  • Patent number: 6306590
    Abstract: Multiphasic microfluidic apparatus for performing product fluid manipulation and separation in a single continuous unit are provided. Related methods, kits, and compositions are also provided.
    Type: Grant
    Filed: June 8, 1998
    Date of Patent: October 23, 2001
    Assignee: Caliper Technologies Corp.
    Inventors: Tammy Burd Mehta, Anne R. Kopf-Sill
  • Patent number: 6303326
    Abstract: The present invention includes the characterization of the major salivary protein or enzyme of the corn earworm Helicoverpa zea for triggering resistance to bacterial blight and frogeye leafspot in soybeans and for triggering resistance to insects in tomatoes. The invention includes an enzyme or a novel protein secreted from the salivary glands of certain insects including the saliva of species belonging to the order Hymenoptera and Lepidoptera. The regurgitant of Helocoverpa zea obtained from the functional salivary glands contains a protein that possesses glucose oxidase activity. The amino acid sequence of the protein is unique and when the protein is applied to plants, it triggers disease and insect resistance systematically. The physical and kinetic attributes of the enzyme are a pH of 7.0, a pI of 4.4 and a molecular weight of 88 kd. The km and Vmax of the enzyme for glucose is 26.9 mmol and 26.7 &mgr;mol min−1 mg−1, respectively.
    Type: Grant
    Filed: December 3, 1998
    Date of Patent: October 16, 2001
    Assignee: University of Arkansas
    Inventors: Gary W. Felton, Mary C. Mathews, Jianlong Bi, John B. Murphy
  • Patent number: 6255477
    Abstract: Magnetic glass particles are prepared containing a magnetic core coated with a glass layer having a substantially pore-free glass surface. The particles are used for separating biological material such as nucleic acids. A preferred process of preparing the particles is by forming a mixture of magnetic cores with a sol formed from an alcohol and a metal alkoxide, spray-drying the mixture to coat the cores with a layer of gelled sol, and heating the coated cores to obtain the magnetic glass particles. Preferably, the particles have an average particle size of less than 100 &mgr;m and any pores of the glass surface have a diameter of less than 10 nm. The magnetic core may be a composite material containing a mica core and magnetite particles immobilized on the mica core, and the glass layer may contain boron oxide. Magnetic core materials include magnetite (Fe3O4) and Fe2O3.
    Type: Grant
    Filed: March 11, 1998
    Date of Patent: July 3, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Jörg Kleiber, Thomas Walter, Herbert Harttig, Christoph Lesniak, Martin Mennig, Michael Riedling, Helmut Schmidt
  • Patent number: 6255468
    Abstract: MPROT12 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing MPROT12 polypeptides and polynucleotides in therapy, and diagnostic assays for such.
    Type: Grant
    Filed: May 5, 1999
    Date of Patent: July 3, 2001
    Assignee: SmithKline Beecham plc
    Inventors: Christopher Donald Southan, Joanne Rachel Evans
  • Patent number: 6251623
    Abstract: A quick assay method for the activity of a plant-derived, asparagine residue-specific endoprotease is disclosed which comprises measuring fluorescence generated by a fluorescence quenching substrate split by an asparagine residue-specific endoprotease. The fluorescence quenching substrate comprises an oligopeptide having in its amino acid sequence at least one asparagine residue, whose C-terminal side is other than an isoleucine residue, a leucine residue, or a valine residue, wherein the oligopeptide has a 7-methoxycoumarin-4-yl-acetyl group arranged on its N-terminal side and a 2,4-dinitrophenyl group arranged on its C-terminal side.
    Type: Grant
    Filed: October 1, 1999
    Date of Patent: June 26, 2001
    Assignee: Director of National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
    Inventors: Masaomi Arahira, Chikafusa Fukazawa
  • Patent number: 6251691
    Abstract: A method and apparatus for the manipulation of colloidal particles and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.
    Type: Grant
    Filed: October 26, 1998
    Date of Patent: June 26, 2001
    Assignee: Bioarray Solutions, LLC
    Inventor: Michael Seul
  • Patent number: 6242207
    Abstract: A dry chemistry reagent matrix composition is provided containing a matrix material and a reagent composition containing 3-methyl-6(sulfonate salt)-benzothiazolinone-(2)-hydrazone (MBTH-S), N-ethyl-N-(3-sulfopropyl)aniline, and an oxidase enzyme or a peroxidase enzyme or a mixture thereof. The dry chemistry reagent matrix composition is useful in reagent test strips for determining the presence or concentration of an analyte in a fluid sample, such as blood.
    Type: Grant
    Filed: October 4, 1999
    Date of Patent: June 5, 2001
    Assignee: Amira Medical
    Inventors: Joel S. Douglas, Karen R. Drexler, John M. Gleisner, John H. Priest
  • Patent number: 6214975
    Abstract: A chemiluminescence-based gel assay may be used as an indicator of fermenter health. The assay is performed using a sample of fermentation medium essentially directly from the fermenter (with no need for complicated purification of the sample before performing the assay). Results from the assay may be correlated with the final product purification yield of a given protein/purification protocol system. This may then be used as a predictor of purification yield before actually performing the purification.
    Type: Grant
    Filed: December 17, 1999
    Date of Patent: April 10, 2001
    Assignee: Bayer Corporation
    Inventors: Michael Zachariou, Jonathan Mazer, Hyun J. Park, Charles Olson
  • Patent number: 6200791
    Abstract: A process for purifying thrombin-like proteases from snake venoms is described, which consists in freeing the proteases from impurities in three chromatographic steps: a) affinity or anion exchange, b) adsorption onto a glass matrix at alkaline pH values, and c) size exclusion gel or glass matrix at acidic pH values.
    Type: Grant
    Filed: August 17, 1998
    Date of Patent: March 13, 2001
    Assignee: Knoll Aktiengesellschaft
    Inventors: Margarete Schwarz, Wolfgang Zahn
  • Patent number: 6156555
    Abstract: A purified enzyme-I is obtained that participates in C-terminal amidation by acting on a peptide C-terminal glycine adduct to form a peptide C-terminal .alpha.-hydroxyglycine adduct. The enzyme has an optimum pH of about 5 to 7, an optimum temperature of 25 to 40.degree. C. and a molecular weight of about 25 kDa or about 36 kDa, and metal ions and ascorbic acid act as a cofactor. A purified enzyme-II is obtained that participates in C-terminal amidation by acting on the peptide C-terminal .alpha.-hydroxyglycine adduct to produce a C-terminal amidated compound. The enzyme has an optimum pH of about 5 to 6, an optimum temperature of 15 to 35.degree. C. and a molecular weight of about 40 kDa or about 43 kDa. Enzyme-I does not act on the peptide C-terminal .alpha.-hydroxyglycine adduct and enzyme-II does not act on the peptide C-terminal glycine adduct. The enzymes may be purified from a biological material such as horse serum by affinity chromatography using a peptide C-terminal glycine adduct as a ligand.
    Type: Grant
    Filed: October 14, 1998
    Date of Patent: December 5, 2000
    Assignee: Shiseido Company Ltd.
    Inventors: Toshii Iida, Toshihiko Kaminuma, Yuka Fuse, Masahiro Tajima, Mitsuo Yanagi, Hiroshi Okamoto, Jiro Kishimoto, Ohji Ifuku, Ichiro Kato
  • Patent number: 6103502
    Abstract: A peptide such as in a cell-free fermentation medium is ultrafiltered with a membrane having a molecular weight cut-off of at least approximately two times greater than the molecular weight of the peptide. The peptide is retained by the membrane, and is desalinated and concentrated to provide prepurification of the peptide. A preferred temperature for ultrafiltration is about 5 to about 15.degree. C. Permeate flow rate may be about 10 l/m.sup.2 /h to about 35 l/m.sup.2 /h, and permeate conductivity may be less than about 10 mS/cm. The permeate may be recycled until peptide concentration is constant. A cell-free fermentation medium containing hirudin from a recombinant microorganism such as Saccharomyces cerevisiae is ultrafiltered with a membrane having a molecular weight cut-off of about 20 kD to about 30 kD.
    Type: Grant
    Filed: November 22, 1996
    Date of Patent: August 15, 2000
    Assignee: Aventis Pharma Deutschland GmbH
    Inventors: Jorg Moller, Frank Richard
  • Patent number: 6080564
    Abstract: A method is presented for inactivating undesired acid labile proteases which are expressed into the culture medium simultaneously with desired enzymes by Aspergillus species when Aspergillus species is used as the host for recombinantly producing enzymes. The method involves incubating culture medium which contains a mixture of the desired recombinantly produced enzyme and the undesired acid labile protease which have been expressed therein at pH values below 4.5 and at temperatures from 2.degree. C. to 75.degree. C. for at least 20 seconds.
    Type: Grant
    Filed: May 12, 1998
    Date of Patent: June 27, 2000
    Assignee: Novo Nordisk A/S
    Inventors: Mads Aage Laustsen, Stig Nielsson