Multiple Layering Of Reactants Patents (Class 435/969)
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Patent number: 10365284Abstract: This invention relates to a kit for detecting a target material and a method of detecting a target material using the same.Type: GrantFiled: November 8, 2017Date of Patent: July 30, 2019Assignee: ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTEInventors: Hyo-Bong Hong, Seung-Min Choi, Jae-Chan Jeong
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Patent number: 8993235Abstract: The present invention contemplates use of encapsulated aqueous and non-aqueous reagents, solutions and solvents and their use in laboratory procedures. These encapsulated aqueous or non-aqueous reagents, solutions and solvents can be completely contained or encapsulated in microcapsules or nanocapsules that can be added to an aqueous or non-aqueous carrier solution or liquid required for medical and research laboratory testing of biological or non-biological specimens.Type: GrantFiled: July 21, 2014Date of Patent: March 31, 2015Inventor: Lee H. Angros
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Patent number: 8785124Abstract: The present invention contemplates use of encapsulated aqueous and non-aqueous reagents, solutions and solvents and their use in laboratory procedures. These encapsulated aqueous or non-aqueous reagents, solutions and solvents can be completely contained or encapsulated in microcapsules or nanocapsules that can be added to an aqueous or non-aqueous carrier solution or liquid required for medical and research laboratory testing of biological or non-biological specimens.Type: GrantFiled: July 17, 2013Date of Patent: July 22, 2014Inventor: Lee H. Angros
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Patent number: 8569078Abstract: The present invention provides novel compositions of binding moiety-nanoparticle conjugates, aggregates of these conjugates, and novel methods of using these conjugates, and aggregates. The nanoparticles in these conjugates can be magnetic metal oxides, either monodisperse or polydisperse. Binding moieties can be, e.g., oligonucleotides, polypeptides, or polysaccharides. Oligonucleotide sequences are linked to either non-polymer surface functionalized metal oxides or with functionalized polymers associated with the metal oxides. The novel compositions can be used in assays for detecting target molecules, such as nucleic acids and proteins, in vitro or as magnetic resonance (MR) contrast agents to detect target molecules in living organisms.Type: GrantFiled: May 3, 2010Date of Patent: October 29, 2013Assignee: The General Hospital CorporationInventors: Lee Josephson, Ralph Weissleder, J. Manuel Perez
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Patent number: 8399261Abstract: A lateral flow test system together with methods for its use in the detection of one or more analytes, or components, of interest within a sample, such as a biological sample, is provided. The system comprises a liquid formulation of a gold conjugate and a lateral flow assay device that does not include a conjugate pad having conjugate dried thereon.Type: GrantFiled: June 27, 2008Date of Patent: March 19, 2013Assignee: Inbios International, Inc.Inventors: Mazbahul Kabir, Syamal Raychaudhuri, James William Needham, Stanislaw Morkowski
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Patent number: 8354235Abstract: The invention concerns a method for increasing the dynamic measuring range of especially immunological test elements in particular immunological chromatography test strips that can be evaluated optically that are based on specific binding reactions. The invention enables the dynamic measuring range of test elements based on specific binding reagents, especially of immunological test elements to be shifted towards higher analyte concentrations without impairing the lower detection limit.Type: GrantFiled: November 7, 2006Date of Patent: January 15, 2013Assignee: Roche Diagnostics Operations, Inc.Inventors: Ludger Bodenbach, Juergen Schaeffler, Juergen Spinke
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Patent number: 8101429Abstract: This invention is directed to a lateral flow assay for detecting the presence of an analyte in a liquid test sample. The lateral flow assay represents an improvement in the ability to accurately and with high fidelity to detect the presence or absence of a target analyte in a liquid sample, in part, by encompassing a reference region of immobilized, non-diffusible analyte that allows for detection of any factors that interfere with the interaction and binding of the analyte to the labeled capture reagent. Any influences on the interaction and binding of the analyte that is free in solution in the liquid test sample to its complementary labeled reagent will be encountered in parallel in the binding between the immobilized analyte in reference region to the labeled reagent as it diffuses through the reference region. In one embodiment, the lateral flow assay of the invention is a urine-based human Chorionic Gonadotropin (hCG) assay.Type: GrantFiled: June 3, 2004Date of Patent: January 24, 2012Assignee: Siemens Healthcare Diagnostics Inc.Inventors: Gary H. Krauth, David J. Ledden
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Patent number: 8071322Abstract: The invention provides a method of identifying an antigen that is present in different amounts in two different samples. In general, the methods involve generating a first and second distinguishably labeled population of antibodies that reactive to the two samples, contacting the first and second labeled populations of antibodies with a plurality of antigens; and identifying any resultant antigens that are differentially bound by the first and second populations of antibodies. The antigens may be on the surface of cells e.g., animal cells, or on a solid support. Once identified, the nucleic acid encoding an antigen of interest may be identified and sequenced to reveal the identity of the antigen of interest. Kits for performing the methods are also provided. The methods find most use in medical and research applications, in particular, for identifying cell surface targets for immunotherapy and drug discovery.Type: GrantFiled: November 10, 2003Date of Patent: December 6, 2011Assignee: Epitomics, Inc.Inventor: Dongxiao Zhang
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Patent number: 7989217Abstract: The subject invention is an immunoassay for the semi-quantitative test kit for determination of human chrionic gonadtropin (hCG) in fluid sample (such as urine) as an aid in the diagnosis of a certain stage of pregnancy. The test device includes five strips having each having a dipping end or sample ends where sample can be applied. Results are indicated by coloration of two bands across a clear area of the strips, one band being coated with a reagent such as hCG antigens and the other with a reagent such as goat/rabbit polyclonal antibody gold conjugate. The combination of color indications on the bands provides the test results.Type: GrantFiled: March 29, 2007Date of Patent: August 2, 2011Assignee: AmeriTek USA, Inc.Inventors: Hsiao-Ching Yee, Kuo-Ching Yee, Hsian-Pei Yee
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Patent number: 7829350Abstract: The present invention provides novel compositions of binding moiety-nanoparticle conjugates, aggregates of these conjugates, and novel methods of using these conjugates, and aggregates. The nanoparticles in these conjugates can be magnetic metal oxides, either monodisperse or polydisperse. Binding moieties can be, e.g., oligonucleotides, polypeptides, or polysaccharides. Oligonucleotide sequences are linked to either non-polymer surface functionalized metal oxides or with functionalized polymers associated with the metal oxides. The novel compositions can be used in assays for detecting target molecules, such as nucleic acids and proteins, in vitro or as magnetic resonance (MR) contrast agents to detect target molecules in living organisms.Type: GrantFiled: August 19, 2008Date of Patent: November 9, 2010Assignee: The General Hospital CorporationInventors: Lee Josephson, Ralph Weissleder, J. Manuel Perez
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Patent number: 7820402Abstract: The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase.Type: GrantFiled: September 19, 2005Date of Patent: October 26, 2010Assignee: Fuji Photo Film Co., Ltd.Inventors: Yoshiki Sakaino, Hitomi Ito, Toshihiro Mori, Osamu Seshimoto, Toshihisa Ito, Yoshikazu Amano
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Patent number: 7816090Abstract: A method of analyzing a sample for one or more analytes of interest includes: providing an element having a base layer; a layer containing streptavidin; a spreading layer, wherein the streptavidin may or may not be in the spreading layer; providing immunocomponents and labeled immunoreactants which may be in the spreading layer or may be combined with the sample; dispensing the sample, and optionally the immunocomponents and a labeled immunoreactant onto the spreading layer at three or more areas, wherein each center of the three or more areas is substantially equidistance to the center formed by the three or more areas, and wherein each area contacts an adjacent area such that wash fluid flow in any direction will contact sample; washing the sample and label by directing the wash fluid at the center formed by the three or more areas, whereby the wash fluid equally flows over each of the three or more areas; and taking a measurement at each three or more areas to determine the presence or concentration of theType: GrantFiled: January 10, 2007Date of Patent: October 19, 2010Assignee: Ortho-Clinical Diagnostics, Inc.Inventor: Merrit N. Jacobs
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Patent number: 7763433Abstract: A method for the determination of a target analyte in a sample, said method comprising: a) applying a liquid sample suspected of containing said target analyte to a solid support capable of allowing lateral flow of liquid there through, which support has diffusibly arranged thereon, (i) either (a) a labeled binding partner for said analyte or (b) a labeled analogue of said analyte, and (ii) a labeled control reagent; b) allowing the sample, labeled binding partner for said analyte or a labeled analogue of said analyte and labeled control reagent to flow through a detection zone on said solid support; c) allowing the sample, labeled binding partner for said analyte or a labeled analogue of said analyte and labeled control reagent to flow through a control zone; and d) detecting the labeled density in the detection zone and the label density in the control zone and comparing these densities.Type: GrantFiled: June 30, 2005Date of Patent: July 27, 2010Assignee: Forsite Diagnostics LimitedInventors: Christopher Danks, Jonathan Richard Flint
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Patent number: 7718444Abstract: This invention provides a simple detection method with excellent sensitivity and specificity, which allows prompt detection of an analyte by allowing a labeled reagent to effectively react with the analyte in the process of treating an analyte-containing specimen, such as during removal of impurities, a detection apparatus, and a detection kit. This method for detecting an analyte in specimens comprises steps of: bringing a labeled reagent containing a ligand that specifically binds to the analyte into contact with a specimen; and supplying the mixture of the specimen and the labeled reagent to a solid-phase support onto which a capture reagent that specifically binds to the analyte has been immobilized, wherein the step of bringing the specimen into contact with the labeled reagent is carried out at a site that is not on the solid-phase support and that is detached from the solid-phase support.Type: GrantFiled: January 27, 2005Date of Patent: May 18, 2010Assignee: Denka Seiken Co., Ltd.Inventors: Kazuyuki Takizawa, Ryo Shida
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Patent number: 7662560Abstract: Described is a method for discriminating complex biological samples using an array of discrete biological sensing elements immobilized onto a solid support in which constituents bound to the sensor array is directly determined by measuring the mass increase on the surface; data analysis of said method is performed using neutral network or statical based pattern recognition techniques. In a preferred embodiment the liquid sample is tested for the presence of soluble constituent(s) by contacting said sample with said sensor array under specific conditions, removing unbound sample constituent(s), determining the mass increase on the surface and comparising said mass increase data with a reference standard using pattern recognition software.Type: GrantFiled: January 25, 2005Date of Patent: February 16, 2010Inventors: Michael Mecklenburg, Bengt Danielsson, Fredrick Wingvist
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Patent number: 7510881Abstract: A method and kit for quantitatively and/or qualitatively detecting one or more components in samples, including the use of metal-particle labelled reagents and an antibody conjugate are disclosed. The components are capable of binding to a probe. A kit and method for staining components in cell and tissue sections, based upon the detection method, are also disclosed.Type: GrantFiled: August 25, 2003Date of Patent: March 31, 2009Inventors: Marc Ramael, Jean-Paul Sanders
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Patent number: 7329536Abstract: An apparatus comprising one or more piezoelectric mass sensors for use in diagnostic and analytic processes, in particular for immunochemical detection of diagnostically relevant analytes in real time, is described. Each piezoelectric mass sensor comprises a piezoelectric crystal with a receptor surface which has immobilized thereon a lawn of recombinant antibodies comprising single VH chain or single-chain Fv (scFv) polypeptides specific for a particular antigen. Binding of antigen to the recombinant antibodies results in a change in mass on the receptor surface which is detected as a change in resonant frequency. In a preferred embodiment, the receptor layer is a precious metal such as gold which facilitates self-assembly of the recombinant antibodies into a lawn on the receptor surface via a cysteine residue at the carboxy terminus of the attachment polypeptide.Type: GrantFiled: June 4, 2004Date of Patent: February 12, 2008Assignees: Oakland University, Vanderbilt UniversityInventors: Xiangqun Zeng, Gabrielle Stryker, Raymond L. Mernaugh
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Patent number: 7326578Abstract: It has been found that a urine test for ovarian reserve can be provided which is equivalent in sensitivity to current laboratory methods which determine ovarian reserve based on FSH levels in serum. A lateral flow device for indicating ovarian reserve is disclosed. The device has an application zone for receiving a urine sample, a labelling zone containing label which binds to FSH in the sample, and a detection zone where FSH-bound label is retained. The detection zone has a threshold value which corresponds to a serum FSII level of between 5-20 mIU/ml (e.g. 10 mIU/ml). A test result above the threshold value indicates diminished ovarian reserve.Type: GrantFiled: February 14, 2002Date of Patent: February 5, 2008Assignee: Genosis (UK) LimitedInventors: Paul North Bateman, Simon John Rattle
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Patent number: 7314763Abstract: A fluidics-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a self-calibrated magnetic binding assay format (e.g., sandwich, competitive, etc.) that includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating a calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional (e.g., directly or inversely) to the intensity of the detection signal calibrated by the intensity of the calibration signal. It has been discovered that the fluidics-based device of the present invention provides an accurate, inexpensive, and readily controllable method of determining the presence of an analyte in a test sample.Type: GrantFiled: August 27, 2002Date of Patent: January 1, 2008Assignee: Kimberly-Clark Worldwide, Inc.Inventors: Xuedong Song, Rosann Kaylor, Shawn Feaster, Kaiyuan Yang
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Patent number: 7297554Abstract: A competitive immunoassay, dye and method for rapidly detecting the presence of one or more target ligands within a fluid sample suspected of containing such ligand or ligands. The immunoassay comprises a protein-binding membrane, a first absorbent pad, a second absorbent pad, and a third absorbent pad. The protein-binding membrane has at least two regions of antibodies bound thereto for detecting dissimilar ligands. The second absorbent pad has formed therein a colloidal gold tracer having one, and preferably two or more ligand analog protein complexes adhering thereto. To utilize the system, the immunoassay strip is placed within a fluid sample. To the extent the target ligand is absent, a visual indicator will be provided signaling such absence. To the extent the target ligand is present at or above a threshold level, no such visual signal will be produced.Type: GrantFiled: October 28, 2003Date of Patent: November 20, 2007Assignee: Microdiagnostics, Inc.Inventors: Roy Chung, June Shozi
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Patent number: 7267992Abstract: A method is provided to determine the presence of an analyte in a sample. The method provides an element with a sample application zone, a detection zone, a zone containing immobilized analyte or analyte analogue, a conjugate that has a first bioaffine binding partner capable of a specific binding reaction with the analyte and a first detectable label, and a universal conjugate, which can be detached by liquid and comprises a second bioaffine binding partner and a visually detectable label. The second bioaffine binding partner is capable of a specific binding reaction with the first detectable label. The method also includes contacting the sample application zone with the sample, contacting the sample with the conjugate and the universal conjugate to react the analyte with the and the universal conjugate to form a detection complex, and determining the presence of the detection complex in the detection zone of the element.Type: GrantFiled: February 7, 2006Date of Patent: September 11, 2007Assignee: Roche Diagnostics GmbHInventors: Ada Goerlach-Graw, Reiner Schlipfenbacher
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Patent number: 7179660Abstract: A polysaccharide coated carrier having a coating of at least two successive layers of polysaccharide. The first polysaccharide layer spontaneously associates with a second polysaccharide layer and, optionally, the carrier. Each successive layer of polysaccharide spontaneously associates with a preceding layer. Spontaneous association occurs due to the presence of oppositely charged functional groups on each layer of polysaccharide or due to a spontaneous reaction between the functional groups the layers. The carrier may be any surface such as a tube, microtitration plate, bead, particle or the like and is suitable for use in diagnostic or therapeutic methods.Type: GrantFiled: March 6, 2000Date of Patent: February 20, 2007Assignee: Dade Behring Marburg GmbHInventors: Hrair Kirakossian, John S. Pease, Carsten Schelp, Marcel R. Pirio, Uwe Stöhr, Andreas Wiegand
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Patent number: 7144742Abstract: This invention provides solid phase specific binding lateral flow assay methods, devices and kits for quantitating high and low molecular weight analytes. The methods and devices of the invention employ labelled reagents which are either analyte analogs or complementary specific binding pair members for the analyte and a novel arrangement of capture zones comprising immobilized specific binding substances for either the analyte or the labelled reagent to effect bound from unbound labelled reagent as a function of analyte concentration. The capture zones are disposed on a non-bibulous matrix defining a flow path from a sample receiving zone to the capture zone. The devices of this invention also include multilane flow paths and multiple capture zones to quantitate analyte.Type: GrantFiled: December 17, 2004Date of Patent: December 5, 2006Assignee: Quidel CorporationInventors: Hans Boehringer, Gerald Rowley, Allan D. Pronovost
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Patent number: 7026002Abstract: The invention concerns an element for the determination of an analyte in a liquid by means of a specific binding reaction of two bioaffine binding partners containing in or on material which enables liquid transport between the zones. The element comprises a sample application zone; a detection zone located downstream thereof that is devoid of binding reagents and is the last zone on the element that allows liquid transport; a zone containing immobilized analyte or analyte analogue between the sample application zone and the detection zone; and impregnated conjugate that can be detached by liquid located in the sample application zone or upstream or downstream thereof composed of a bioaffine binding partner capable of a specific binding reaction with the analyte to be determined and a detectable label.Type: GrantFiled: June 15, 2000Date of Patent: April 11, 2006Assignee: Roche Diagnostics GmbHInventors: Ada Goerlach-Graw, Reiner Schlipfenbacher
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Patent number: 6951763Abstract: Methods of detecting antibodies to one or more glycosphingolipid(s) of interest in a sample are disclosed which comprise using a solid-phase reactant having carbonyl groups attached theron, and the glycosphingolipid(s) of interest linked to the solid-phase reactant by an amide bond between an amino group of the glycosphingolipid of interest and a carbonyl group attached to the solid-phase reactant. The methods of detecting antibodies to glycosphingolipid(s) of interest can be used in methods of diagnosing autoimmune diseases in an individual.Type: GrantFiled: June 10, 2003Date of Patent: October 4, 2005Assignee: Athena Diagnostics, Inc.Inventors: Normand J. Robichaud, Louis P. Kertiles
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Patent number: 6951760Abstract: The invention relates to a method for detection of an analyte in a test sample by a specific binding reaction among the analyte, a specific binding partner for the analyte, and an (immuno)reactant provided with a label, characterized in that the label is a lanthanide ion-ligand complex wherein the lanthanide ion is neodymium(III) ion (Nd3+), ytterbium(III) ion (Yb3+), or erbium(III) ion (Er3+) and the ligand comprises or is in contact with a sensitizing moiety which absorbs in the 400-1000 nm region, and preferably in the 400-800 nm region.Type: GrantFiled: February 4, 2000Date of Patent: October 4, 2005Inventor: Johannes Willem Hofstraat
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Patent number: 6924153Abstract: This invention provides solid phase specific binding lateral flow assay methods, devices and kits for quantitating high and low molecular weight analytes. The methods and devices of the invention employ labelled reagents which are either analyte analogs or complementary specific binding pair members for the analyte and a novel arrangement of capture zones comprising immobilized specific binding substances for either the analyte or the labelled reagent to effect bound from unbound labelled reagent as a function of analyte concentration. The capture zones are disposed on a non-bibulous matrix defining a flow path from a sample receiving zone to the capture zone. The devices of this invention also include multilane flow paths and multiple capture zones to quantitate analyte.Type: GrantFiled: March 6, 1997Date of Patent: August 2, 2005Assignee: Quidel CorporationInventors: Hans Boehringer, Gerald Rowley, Allan Pronovost
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Patent number: 6913935Abstract: A method for the assay of N samples each containing a compound to be tested, comprises providing N reaction vessels each containing a population of carrier beads and other reagents for performing the assay, where N is at least 2 e.g. 80-4000. Each population of carrier beads is distinguishable from every other population. After adding the samples to the reaction vessels and performing the assays, the contents of all the reaction vessels are mixed and subjected to analysis by flow cytometry. By means of flow cytometry, each carrier bead is rapidly analysed to identify its population and also to determine the presence or concentration or biological activity of the compound to be tested.Type: GrantFiled: December 3, 1998Date of Patent: July 5, 2005Assignee: Amersham Biosciences UK LimitedInventor: Nicholas Thomas
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Patent number: 6884597Abstract: A method for simply and conveniently detecting acetyltransferase and deacetylase activities of proteins by executing an acetylation reaction of a peptide substrate with an acetyltransferase, or a deacetylation reaction of an acetylated peptide substrate with a deacetylase, and after the completion of these reactions, detecting the acetyl group bound to the peptide substrate by using an anti-acetylated peptide antibody. This system for detecting acetyltransferase and deacetylase activities using the anti-acetylated peptide antibody enables screening inhibitors or enhancers of acetyltransferase and deacetylase. A system for screening deacetylase inhibitors or acetyltransferase enhancers using cultured cells is also provided.Type: GrantFiled: July 18, 2000Date of Patent: April 26, 2005Assignee: Medical & Biological Laboratories, Co., Ltd.Inventors: Yoichi Taya, Katsuyuki Tamai, Toshiaki Miyazaki
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Patent number: 6884628Abstract: Multifunctional, polyionic copolymers with molecular architectures and properties optimized for specific applications are synthesized on/or applied to substrate surfaces for analytical and sensing purposes. The coatings are particularly useful for suppression of non-specific interaction, adsorption or attachment of molecular or ionic components present in an analyte solution. Chemical, biochemical or biological groups that are able to recognize, interact with and bind specifically to target molecules in the material containing the analyte to be detected can be coupled to, integrated into, or absorbed to the multifunctional copolymers. These multifunctional copolymer coatings are compatible with a variety of different established methods to detect, sense and quantify the target molecule in an analyte.Type: GrantFiled: April 28, 2000Date of Patent: April 26, 2005Assignees: Eidgenossische Technische Hochschule Zurich, Universitat ZurichInventors: Jeffrey A. Hubbell, Marcus Textor, Donald L. Elbert, Stephanie Finken, Rolf Hofer, Nicholas D. Spencer, Laurence Ruiz-Taylor
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Patent number: 6818455Abstract: An analytical test device useful for example in pregnancy testing, comprises a hollow casing (500) constructed of moisture-impervious solid material, such as; plastics materials, containing a dry porous carrier (510) which communicates indirectly with the exterior of the casing via a bibulous sample receiving member (506) which protrudes from the casing such that a liquid test sample can be applied to the receiving member and permeate therefrom to the porous carrier, the carrier containing in a first zone a labelled specific binding reagent is freely mobile within the porous carrier when in the moist state, and in a second zone spatially distinct from the first zone unlabelled specific binding reagent for the same analyte which unlabelled reagent is permanently immobilised on the carrier material and is therefore not mobile in the moist state, the two zones being arranged such that liquid sample applied to the porous carrier can permeate via the first zone into the second zone, and the device incorporating meType: GrantFiled: February 28, 2001Date of Patent: November 16, 2004Assignee: Inverness Medical Switzerland GmbHInventors: Keith May, Michael Evans Prior, Ian Richards
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Patent number: 6723516Abstract: A method is provided for continuously monitoring for the presence or quantity of an analyte in a flowing liquid stream. The method involves binding an analyte-specific receptor species to the surface of a piezoelectric substrate, contacting the surface bound receptor species with the flowing liquid stream and quantitating the presence of the analyte. A novel apparatus for detecting the presence of an analyte in a liquid chromatography eluant is provided as well.Type: GrantFiled: October 24, 1996Date of Patent: April 20, 2004Assignee: Agilent Technologies, Inc.Inventors: May Tom-Moy, Thomas P. Doherty, Richard L. Baer, Darlene J. Spira-Solomon
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Patent number: 6686208Abstract: The invention relates to devices and methods for carrying out quantitative fluorescence immunoassays using evanescent field excitation. Light from at least one light source is directed onto the boundary between two media which have differing refractive indices. The light source emits practically monochromatic light with a wavelength suitable for exciting a marking substance. The light is directed onto a boundary surface disposed between an optically transparent base plate, the refractive index of which is greater than that of the material above the boundary surface, and a receiving region for the sample. The receiving region is covered with a covering plate on the side disposed opposite the base plate, there being arranged between the base plate and covering plate at least one functional layer. A detector for detecting the fluorescent light is disposed on the same side of the base plate as the light source.Type: GrantFiled: August 6, 2002Date of Patent: February 3, 2004Assignee: Institut fur Chemo- und Biosensorik Munster e.V.Inventors: Markus Meusel, Dieter Trau, Andreas Katerkamp
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Patent number: 6670198Abstract: A test piece for use in biological analyses includes a plurality of different known specific binding substances disposed in predetermined positions on a substrate. The specific binding substances are disposed on a plurality of surfaces provided by the substrate and arranged in the direction of thickness of the substrate.Type: GrantFiled: May 8, 2001Date of Patent: December 30, 2003Assignee: Fuji Photo Film Co., Ltd.Inventor: Toshihito Kimura
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Patent number: 6638728Abstract: New protein coated surfaces, which have a high capacity for capturing target molecules, thus yielding assays with enhanced sensitivity, are disclosed. Surfaces prepared according to the present invention contain a coating consisting essentially of streptavidin, avidin or “NeutrAvidin” in polymeric form, wherein polymerization has been controlled to an extent such that the polymer is predominantly dimers, trimers and tetramers of the native molecule.Type: GrantFiled: June 15, 2001Date of Patent: October 28, 2003Assignee: Pierce Biotechnology, Inc.Inventors: Surbhi Desai, Mark Rickerd, Ineabel Horneij
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Patent number: 6534270Abstract: Disclosed are biochips having a high detecting sensitivity with readiness in fabrication of microarray, and a method for fabricating the same, in which a solid support wound with fibers is immersed in a solution containing biomolecules to immobilize the biomolecules onto the fiber, and the individual fibers with the biomolecules immobilized thereon are straightened and arranged. The arranged fibers are embedded with a defined material and cut in a direction perpendicular to the lengthwise arrangement direction of the fibers to obtain thin chips. The chips are placed on a substrate to remove the material used for embedding and thereby remain fibers with the immobilized biomolecules on the substrate. This biochip fabrication method immobilizes a great number of biomolecules onto the fibers having a large surface area to enhance the detection sensitivity and allows production of a great number of substrates with an array of biomolecules immobilized simultaneously.Type: GrantFiled: April 12, 2001Date of Patent: March 18, 2003Assignee: LG Electronics Inc.Inventors: Su Hyeon Kim, Je Kyun Park, Tae Han Kim
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Patent number: 6531323Abstract: An agglutination assay method for quantitatively determination of an analyte in a liquid sample using particles bearing an anti-analyte. A non-fluid substance which retains the particles while suppressing the diffusion of the particles therein is used as a medium which is to be a place where the agglutination of the particles takes place. Upon analysis, a solation agent is added to the non-fluid substance medium to increase the fluidity of the non-fluid substance, thereby the particles bearing the anti-analyte can diffuse in the medium to cause the agglutination with the analyte. Preferably, the solation agent is added to the non-fluid medium together with the sample. The non-fluid substance medium containing the particle-labeled anti-analyte can be stored with a higher stability in the dry state. A dry analysis element for enabling such analysis method is also provided.Type: GrantFiled: January 28, 2000Date of Patent: March 11, 2003Assignee: Fuji Photo Film Co., Ltd.Inventors: Hiroshi Shinoki, Yoshikazu Amano
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Patent number: 6521465Abstract: An array bundle is provided for creating multiple arrays for testing. The array bundle is adapted to be cut transversely to form a series of identical arrays of cells. In one embodiment, the array bundle is used in detecting predetermined components from sample mixtures.Type: GrantFiled: September 14, 2001Date of Patent: February 18, 2003Assignee: Unitec Co., Ltd.Inventor: Don Stimpson
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Patent number: 6511798Abstract: Cell based screens for studying drug transport are described and improved methods for the preparation of cell monolayers for use in such screens are disclosed.Type: GrantFiled: March 16, 1999Date of Patent: January 28, 2003Assignee: Zeneca LimitedInventor: Charles Michael Shaw
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Patent number: 6492119Abstract: A macro array comprises a film-shaped hard porous body and a plurality of spots, which contain test substances and are arrayed on the film-shaped hard porous body. The film-shaped hard porous body may be constituted of a surface layer region, which is provided with through-pores having a comparatively small mean pore diameter, and a base layer region, which is provided with through-pores having a comparatively large mean pore diameter. The surface of the film-shaped hard porous body, on which surface the spots are to be arrayed, may be coated with an auxiliary substance for promoting fixation of the test substances to the surface of the film-shaped hard porous body.Type: GrantFiled: September 18, 2000Date of Patent: December 10, 2002Assignee: Fuji Photo Film Co., Ltd.Inventor: Masashi Ogawa
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Patent number: 6461828Abstract: A diagnostic tool is disclosed for accurately and rapidly diagnosing the condition of an ailing organ. Although applicable to numerous organ and organ systems, this application particularly illustrates the concept of conjunctive marker utilization as it relates to diagnosing and distinguishing congestive heart failure. The invention particularly relates to the conjunctive utilization of cardiac Troponin I (cTn-I) and natriuretic peptide, e.g. ANP, pro-ANP, BNP, pro-BNP and CNP as a retrospective tool for diagnosing the underlying mechanism of heart failure and as a prospective analytical device for monitoring disease progression and efficacy of therapeutic agents.Type: GrantFiled: September 4, 2001Date of Patent: October 8, 2002Assignee: Syn X PharmaInventors: Eric B. Stanton, George Jackowski
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Patent number: 6458548Abstract: A container for measurement of cell functions, for use in the determination of a physiologically active substance produced by blood cells, is constituted such that an amount of material capable of inducing production of the physiologically active substance, when extracted by collecting water of a volume equal to a liquid volume to be subjected to measurement, is controlled at a level insufficient to induce production of the physiologically active substance from the blood cells. A container for measurement of cell functions in which a material capable of inducing production of a physiologically active substance in blood when contacted with the blood is accommodated in such a condition as being contactable with blood, and in which an amount of the material capable of inducing production of the physiologically active substance in the container before use is limited to a level insufficient to influence a measured value of the physiologically active substance.Type: GrantFiled: April 1, 1998Date of Patent: October 1, 2002Assignee: Sekisui Kagaku Kogyo Kabushiki KaishaInventors: Koji Kobayashi, Yuji Setoguchi, Kiyoshi Kuriyama
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Patent number: 6458601Abstract: A test piece for use in biological analyses includes a plurality of different known specific binding substances disposed in predetermined positions on a substrate. The specific binding substances are disposed on a plurality of surfaces provided by the substrate and arranged in the direction of thickness of the substrate.Type: GrantFiled: May 16, 2000Date of Patent: October 1, 2002Assignee: Fuji Photo Film Co., Ltd.Inventor: Toshihito Kimura
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Patent number: 6429026Abstract: The invention relates to a novel competitive or non-competitive immunoassay comprising the steps of immobilizing an assay specific component on a solid phase in a reaction well, adding a labelled assay specific component, drying said components, adding the sample containing the marker to be analyzed, allowing the marker to react with the assay specific components, and detecting the signal from the label. Furthermore, the invention relates to a device useful in carrying out the novel assay.Type: GrantFiled: April 8, 1996Date of Patent: August 6, 2002Assignee: Innotrac Diagnostics OyInventors: Kim Sverker Immanuel Pettersson, Timo Nils-Erik Lövgren
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Patent number: 6376195Abstract: A chromatographic strip positive readout binding assay device and method suitable for quick, sensitive and reliable field testing for small molecules such as environmental contaminants, drugs of abuse, therapeutic drugs and hormones. The detectable label is not attached to either the analyte or to the analyte receptor. Low affinity binding pairs can be used in the positive readout binding assay.Type: GrantFiled: February 18, 1999Date of Patent: April 23, 2002Assignee: Strategic Diagnostics Inc.Inventor: James P. Mapes
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Patent number: 6372515Abstract: A test card for drugs of abuse has a thin flat member having the size and shape of a business card. A plurality of immunoassay test strips extend longitudinally from top to bottom at the card and are fastened side by side in parallel on one or both sides of the test card within the outline of the card. Each test strip is reactive to provide a visual indication in response to a particular drug of abuse. The test card thus provides for the simultaneous detection of multiple analytes. Processes are also disclosed for making the drug test card with test strips on one and both sides of the card.Type: GrantFiled: April 21, 1999Date of Patent: April 16, 2002Assignee: American Bio Medica CorporationInventors: Douglas Casterlin, Henry J. Wells, Stan Cipkowski
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Patent number: 6350620Abstract: The invention provides a method for producing a micro-carrier, which includes patterning pluralities of bar code on a mask; exposing the bar code to a substrate coated with photoresist; etching and removing residual photoresist and electroforming to a nickel plate; placing a bead coated with biotin or poly-L-lysine between two-nickel plates, and compressing the bar code on the surface of the bead to form a microcake-like particle with bar code; and combining the particle with the corresponding bio-molecule thereof to produce a micro-carrier with a label. The invention also provides a test method for identifying a bio-molecule, which includes mixing several micro-carriers with the labeled unknown bio-molecules; and identifying the bar code on the micro-carrier via image recognition system, wherein the numbers and types of the known micro-carrier can be flexibly adjusted.Type: GrantFiled: December 1, 2000Date of Patent: February 26, 2002Assignee: Genemaster Lifescience Co., LTDInventors: Rong-Seng Chang, Yu-Chan Chao
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Patent number: RE38688Abstract: A solid diagnostic device for the quantitative determination of substances of biological affinity in biological fluids is described. A process is also described in which the biological fluid is brought into contact with a specific functional sector of the device, the fluid migrates through several functional sectors situated beside one another and containing suitable reagent components, and one or more substances of biological affinity are detected in such functional sectors which contain, for each substance to be detected, at least one combination partner of biological affinity, attached to a solid phase.Type: GrantFiled: March 30, 2001Date of Patent: January 18, 2005Assignee: Dade Behring Marburg GmbHInventors: Heinz-Jürgen Friesen, Gerd Grenner, Hans-Erwin Pauly, Helmut Kohl, Klaus Habenstein, Joseph Stärk
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Patent number: RE39664Abstract: The present invention relates to improved specific binding assay devices comprising a chromatographic medium including a reaction site at which a specific binding reagent is immobilized, a sample application well located adjacent to the chromatographic medium and offset upstream from the reaction site, and liquid absorption blotter offset downstream from the reaction site.Type: GrantFiled: November 21, 1995Date of Patent: May 29, 2007Assignee: Inverness Medical Switzerland GmbHInventors: Julian Gordon, Charles Stuart Gredell Pugh
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Patent number: RE39816Abstract: A diagnostic tool is disclosed for accurately and rapidly diagnosing the condition of an ailing organ. Although applicable to numerous organ and organ systems, this application particularly illustrates the concept of conjunctive marker utilization as it relates to diagnosing and distinguishing congestive heart failure. The invention particularly relates to the conjunctive utilization of cardiac Troponin I (cTn-I) and natriuretic peptide, e.g. ANP, pro-ANP, BNP, pro-BNP and CNP as a retrospective tool for diagnosing the underlying mechanism of heart failure and as a prospective analytical device for monitoring disease progression and efficacy of therapeutic agents.Type: GrantFiled: April 29, 2005Date of Patent: September 4, 2007Assignee: Nanogen Inc.Inventors: Eric B. Stanton, George Jackowski