Kit Patents (Class 435/975)
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Patent number: 8507260Abstract: Disclosed is a lateral flow capillary device and uses thereof comprising a unipath bibulous capillary flow matrix and at least two reservoirs each in fluid communication with the capillary flow matrix wherein a reservoir contacts the capillary flow matrix through a passage having a rim pressing the matrix. The pressure that the rim applies on the matrix prevents leakage of liquids out of the capillary flow matrix at the reservoir/ matrix interface, allowing accurate sequential draining of liquid from the reservoirs.Type: GrantFiled: June 2, 2010Date of Patent: August 13, 2013Assignee: Realbio Technologies, Ltd.Inventors: Sara Alajem, Avraham Reinhartz
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Patent number: 8507216Abstract: The present invention provides a method comprising allowing a reaction of a sample, a reagent containing Factor C, which can be activated by binding with endotoxin, and a synthetic luminescent substrate comprising a luminescent substrate bound to a peptide, for release of the luminescent substrate from the synthetic luminescent substrate, allowing a luminescent enzyme to act on the luminescent substrate released in the luminescent substrate release step, for measurement of the luminescence intensity, and quantifying the level of endotoxin in the sample based on a measured value obtained in the luminescence measuring step, the method enabling endotoxin to be simply and quickly measured at a level that cannot be detected in conventional methods for endotoxin measurement, without use of any dedicated measuring device.Type: GrantFiled: February 6, 2013Date of Patent: August 13, 2013Assignee: Hiroshima UniversityInventors: Akio Kuroda, Kenichi Noda
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Patent number: 8507196Abstract: This invention is in the field of lymphadenopathy virus. This invention relates to a diagnostic means and method to detect the presence of DNA, RNA, or antibodies of the lymphadenopathy retrovirus associated with the acquired immune deficiency syndrome or of the lymphadenopathy syndrome by the use of DNA fragments or the peptides encoded by said DNA fragments. The invention further relates to the DNA fragments, vectors comprising them, and the proteins expressed.Type: GrantFiled: April 23, 1993Date of Patent: August 13, 2013Assignees: Institut Pasteur, Centre National de la Recherche ScientifiqueInventors: Marc Alizon, Francoise Barre Sinoussi, Pierre Sonigo, Pierre Tiollais, Jean-Claude Chermann, Luc Montagnier, Simon Wain-Hobson
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Patent number: 8501427Abstract: The invention relates to antibodies, including monoclonal and polyclonal, or fragments thereof, which discriminate between the histidine and tyrosine isoforms of Complement Factor H and to their use in diagnostic methods and therapeutic treatments relating to Complement Factor H mediated diseases.Type: GrantFiled: January 23, 2008Date of Patent: August 6, 2013Assignee: University College Cardiff Consultants LimitedInventors: Bryan Paul Morgan, Claire Louise Harris, Svellana Hakobyan
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Patent number: 8491914Abstract: Described herein are compositions and methods of use of targeted delivery complexes for delivery of siRNA to a disease-associated cell, tissue or pathogen. The targeted delivery complex comprises a targeting molecule, such as an antibody or fragment thereof, conjugated to one or more siRNA carriers. In preferred embodiments the siRNA carrier is a dendrimer or protamine and the targeting molecule is an anti-cancer antibody, such as hRS7. More preferably, the antibody or fragment is rapidly internalized into the target cell to facilitate uptake of the siRNA. Most preferably, the targeted delivery complex is made by the DNL technique. The compositions and methods are of use to treat a variety of disease states, such as cancer, autoimmune disease, immune dysfunction, cardiac disease, neurologic disease, inflammatory disease or infectious disease.Type: GrantFiled: December 9, 2010Date of Patent: July 23, 2013Assignee: IBC Pharmaceuticals, Inc.Inventors: Chien-Hsing Chang, David M. Goldenberg
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Patent number: 8481707Abstract: This application describes methods and compositions for detecting and treating vimentin-associated neoplasia. Differential methylation of the vimentin nucleotide sequences has been observed in vimentin-associated neoplasia such as colon neoplasia.Type: GrantFiled: June 27, 2008Date of Patent: July 9, 2013Assignee: Case Western Reserve UniversityInventors: Sanford D. Markowitz, Wei-Dong Chen
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Patent number: 8476027Abstract: A binding partner, especially an antibody fragment that specifically recognizes an antigen-antibody immune complex between anti-THC and THC (tetrahydrocannabinol), is disclosed. The binding partner facilitates a non-competitive homogenous immunoassay for detection of cannabis use. A test kit comprising the binding partner is also described. Preferably the immunoassay is applied for roadside testing of saliva from suspected drivers.Type: GrantFiled: June 5, 2009Date of Patent: July 2, 2013Assignee: Teknologian Tutkimuskeskus VTTInventors: Kristiina Takkinen, Hans Söderlund, Timo Pulli
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Patent number: 8475798Abstract: Monoclonal antibodies which can bind to the SdrF protein of Staphylococcus epidermidis are provided which can be useful in the treatment and protection against infection from staphylococcal bacteria such as Staphylococcus epidermidis. The monoclonal antibodies of the invention are advantageous in that they can also recognize binding domains and subdomains of the S. epidermidis SdrF protein in addition to the protein itself. Suitable compositions and passive vaccines based on the monoclonal antibodies of the invention, as well as methods for their use, are also provided.Type: GrantFiled: June 16, 2006Date of Patent: July 2, 2013Assignees: Inhibitex, Inc., The Texas A&M University SystemInventors: Joseph M. Patti, Jeff T. Hutchins, Andrea Hall, Linda Santos, Maria Bowden, Magnus Hook
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Patent number: 8476065Abstract: The present invention is directed to a device comprising (a) a substrate having a surface and (b) an ordered array of posts over the surface, wherein the posts are capable of binding a protein or small molecule ligand, and wherein the pitch between adjacent posts is less than about 100 nm. The invention is also directed to methods for identifying the presence of an analyte in a fluid and to methods for measuring relative binding specificity or affinity between an analyte in a fluid and the posts, using the device of the present invention.Type: GrantFiled: April 13, 2006Date of Patent: July 2, 2013Assignee: The Trustees of Columbia University in the City of New YorkInventors: Michael P. Sheetz, Samuel J. Wind
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Patent number: 8470610Abstract: Analytes in a sample are resolved by retentate chromatography in a procedure involving adsorbing the analytes on a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.Type: GrantFiled: August 17, 2011Date of Patent: June 25, 2013Assignee: Bio-Rad Laboratories, Inc.Inventors: T. William Hutchens, Tai-Tung Yip
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Patent number: 8460934Abstract: The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.Type: GrantFiled: April 3, 2012Date of Patent: June 11, 2013Assignee: Applied Biosystems, LLCInventors: Chien-Wei Chang, Lori Hennessy, Dennis Wang
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Patent number: 8450059Abstract: Provided are quantitative PCR-based compositions and methods for the diagnosis of invasive pulmonary aspergillosis (IPA) in a patient sample, such as bronchoalveolar lavage (BAL) fluid. The methods presented herein involve isolating a patient sample, optionally extracting DNA from the sample, carrying out a quantitative PCR (qPCR) reaction on the sample to generate an amplicon that includes a region of an Aspergillus spp. ribosomal RNA (rRNA) gene, and detecting the PCR amplicon. The present disclosure also provides primers and primer sets for specifically detecting an Aspergillus spp. fungal pathogen in the presence of human ribosomal DNA (rDNA).Type: GrantFiled: April 27, 2010Date of Patent: May 28, 2013Assignee: Fred Hutchinson Cancer Research CenterInventors: David N. Fredricks, Prasanna D. Khot, Daisy L. Ko
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Patent number: 8450463Abstract: Described herein is an in vitro method for the determination of the formation of endothelins in serious diseases, in particular cardiovascular diseases, inflammations, sepsis and cancer, in whole blood, plasma or serum of a human patient, in which relatively long-lived peptide fragments of the processed primary prepro- or proendothelins are determined which contain neither the actual biologically active endothelin nor its direct precursor big endothelin. In particular, disclosed are antibodies and kits for selectively detecting these fragments.Type: GrantFiled: February 16, 2012Date of Patent: May 28, 2013Assignee: B.R.A.H.M.S GmbHInventors: Andreas Bergmann, Joachim Struck
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Patent number: 8444992Abstract: Various improvements to vaccines that include a serogroup C meningococcal conjugate antigen, including: (a) co-administration with acellular B. pertussis antigen; (b) co-administration with an inactivated poliovirus antigen; (c) supply in a kit together with a separate pneumococcal conjugate component, which may be in a liquid form; and (d) use in combination with a pneumococcal conjugate antigen but without an aluminium phosphate adjuvant. A kit may have: (a) a first immunogenic component that comprises an aqueous formulation of a conjugated capsular saccharide from Streptococcus pneumoniae; and (b) a second immunogenic component that comprises a conjugated capsular saccharide from Neisseria meningitidis serogroup C.Type: GrantFiled: September 4, 2012Date of Patent: May 21, 2013Assignee: Novartis Vaccines and Diagnostics GmbHInventor: Astrid Borkowski
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Patent number: 8445664Abstract: This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic acid construct is utilized to carry out the amplification process. In non-linear amplification, a first initial primer or nucleic acid construct is employed with a subsequent initial primer or nucleic acid construct. In other non-linear amplification processes provided by this invention, a first initial primer or nucleic acid construct is deployed with a second initial primer or nucleic acid construct to amplify the specific nucleic acid sequence of interest and its complement that are provided. A singular primer or a singular nucleic acid construct capable of non-linear amplification can also be used to carry out non-linear amplification in accordance with this invention.Type: GrantFiled: October 20, 2005Date of Patent: May 21, 2013Assignee: Enzo Diagnostics, Inc.Inventors: Elazar Rabbani, Jannis Stavrianopoulos, James J. Donegan, Jack Coleman, Marleen Walner
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Patent number: 8440448Abstract: Provided are methods of treating a surface of a food processing facility which has a first population of microorganisms disposed thereon. The method includes disposing on the surface a biofilm containing a second population of microorganisms or a population of microorganisms that can form a biofilm onto the surface of the food processing facility. The growth of the second microbial population and the formation of a biofilm comprising these microorganisms can then inhibit the growth of the first population of microorganisms.Type: GrantFiled: August 14, 2009Date of Patent: May 14, 2013Assignee: University of Georgia Research Foundation, Inc.Inventors: Michael P. Doyle, Tong Zhao
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Patent number: 8435737Abstract: Nucleic acid sequences are provided which in an embodiment provide a primer pair. The primers are capable of amplifying a nucleic acid molecule that indicates the presence of a propane-oxidizing and/or butane-oxidizing microorganism. A method is provided which employs such primers in a process that indicates the presence of such organisms. The method is useful in detecting the presence of petroleum-like products.Type: GrantFiled: June 1, 2011Date of Patent: May 7, 2013Assignee: Cal Poly CorporationInventors: Raul Cano, Christopher Kitts, Brian Jeremy Chan
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Patent number: 8415113Abstract: A procedure for the identification of a functional disorder of the pancreas by the use of parts of all iso-enzymes of the pancreas elastase and of synthetic amino-acid sequences as antigens for obtaining specific antibodies, as well as their use in immuno-chemical test procedures.Type: GrantFiled: September 3, 1999Date of Patent: April 9, 2013Assignee: R-Biopharm AGInventors: Hans-Werner Heinrich, Rainer Kleinert, Udo Meyer, Heinz-Jürgen Wagner
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Patent number: 8399231Abstract: The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus, and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.Type: GrantFiled: October 14, 2010Date of Patent: March 19, 2013Assignee: Applied Biosystems, LLCInventors: Elena Bolchakova, James Rozzelle
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Patent number: 8399204Abstract: The present invention provides a screening method for determining whether a substance of interest is a substance which alters GPR120 mediated cell stimulating activities, comprising using a substance of interest, a biomembrane containing GPR120, or cells containing said biomembrane, and phospholipase or salts thereof. According to a screening method of the present invention, the method can screen substances such as CCK and GLP-1 which are involved in the secretion of hormones in gastrointestinal tracts.Type: GrantFiled: September 1, 2006Date of Patent: March 19, 2013Assignee: Eisai R&D Management Co., Ltd.Inventors: Tomoko Sekiya, Norimasa Miyamoto, Hirokazu Tanaka, Naoko Massaki
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Patent number: 8394627Abstract: A home test kit for detecting fecal blood includes a scoop device for collecting a fecal sample, a luminescent for mixing with the sample, a hollow container for containing the sample, water and the luminescent, or luminol during use of the kit. A base may be provided for supporting the container during testing, and the container may include an integrally formed lid. Detection of the blood in the sample is possible because the luminescent undergoes a light-producing reaction that involves, as a reactant or catalyst, blood or blood components or products. The blood is thus visible, or glows in darkened surroundings. The kit is compact, easy to use, and formed from biodegradable material for easy disposal.Type: GrantFiled: October 8, 2010Date of Patent: March 12, 2013Assignee: Life Fit Testing, LLCInventor: G. Joseph Fitzgerald
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Patent number: 8389678Abstract: Disclosed are cloning and expression of a plurality of protein fragments of virB10, a Type IV Secretion System (TIVSS) in Anaplasma phagocytophilum. Such recombinant protein fragments are useful in the ELISA detection of anaplasma pathogen. The use of same as kits for ELISA is also disclosed.Type: GrantFiled: March 13, 2012Date of Patent: March 5, 2013Assignee: Medical Diagnostic Laboratories, LLCInventors: John G. Hoey, Denise P. Dimitrov, Lisa B. Huang, Martin E. Adelson, Eli Mordechai
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Patent number: 8389699Abstract: Disclosed are methods and kits applicable to sequencing methods, such as Sanger dideoxy sequencing methods. The methods and kits disclosed utilize a cationically charged nucleic acid terminator in combination with a discriminatory polymerase.Type: GrantFiled: October 15, 2009Date of Patent: March 5, 2013Assignee: Applied Biosystems, LLCInventors: Barnett Rosenblum, Steven Menchen
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Patent number: 8389234Abstract: Presented herein are kits for the detection of specific beta-lactamases, including class A serine carbapenemases, metallo-beta-lactamases, AmpC beta-lactamases, and extended-spectrum beta-lactamases (ESBLs). The kits presented herein include kits that permit the detection of the presence of specific beta-lactamases in bacterial samples within as few as 2 to 10 minutes.Type: GrantFiled: November 30, 2011Date of Patent: March 5, 2013Assignee: Becton, Dickinson and CompanyInventors: Mei Yang-Woytowitz, Charles Yu, Timothy Wiles
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Patent number: 8367367Abstract: A method of positively detecting toxic materials within a sample, the method including contacting sub-mitochondrial particles having competent mitochondrial enzymes formed from inner membranes of mitochondria, an electron donor which transmits electrons to an electron transfer system of the sub-mitochondrial particles and the sample, and forming reaction resultants, adjusting a pH of a pH indicator which change color according to a change in pH, adding the pH indicators to each reaction resultant and identifying color changes of the pH indicator. Also provided is a kit for positively detecting toxic materials within a sample.Type: GrantFiled: June 8, 2011Date of Patent: February 5, 2013Assignee: Samsung Electronics Co., Ltd.Inventors: Jung-im Han, Soo-hyung Choi, Soo-suk Lee, Sung-ouk Jung, Joon-shik Park, Ki-eun Kim
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Patent number: 8354235Abstract: The invention concerns a method for increasing the dynamic measuring range of especially immunological test elements in particular immunological chromatography test strips that can be evaluated optically that are based on specific binding reactions. The invention enables the dynamic measuring range of test elements based on specific binding reagents, especially of immunological test elements to be shifted towards higher analyte concentrations without impairing the lower detection limit.Type: GrantFiled: November 7, 2006Date of Patent: January 15, 2013Assignee: Roche Diagnostics Operations, Inc.Inventors: Ludger Bodenbach, Juergen Schaeffler, Juergen Spinke
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Patent number: 8354514Abstract: Described are kits and methods useful for detection of agricultural pathogens in a sample. Genomic sequence information from agricultural pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay and/or an array assay to successfully identify the presence or absence of pathogens in a sample.Type: GrantFiled: July 23, 2010Date of Patent: January 15, 2013Assignee: Lawrence Livermore National Security, LLCInventors: Thomas R. Siezak, Shea Gardner, Clinton Torres, Elizabeth Vitalis, Raymond J. Lenhoff
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Patent number: 8343122Abstract: The invention provides for a urinary monitoring device to monitor for the presence or absence of markers indicative of a urinary tract infection (UTI). The invention also provides for methods of using such a device.Type: GrantFiled: April 23, 2010Date of Patent: January 1, 2013Inventor: Geoffrey H Gorres
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Patent number: 8338125Abstract: The present invention relates to kits for measuring nitric oxide synthase activity.Type: GrantFiled: June 9, 2010Date of Patent: December 25, 2012Assignee: Boehringer Ingelheim International GmbHInventor: Frank Buettner
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Patent number: 8338126Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes.Type: GrantFiled: December 7, 2011Date of Patent: December 25, 2012Inventor: Olga Ornatsky
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Patent number: 8323656Abstract: A dipeptide antigen determinant is disclosed where haptenic Cit and Cys residues are connected by means of a peptide bond having the formula III: where A is —NH2, B is —O—, D is —NH—, F is —NHCO—, or —NR—, where R is hydrogen or acyl, G is —CONH—, or —COOR, wherein R is alkyl, E is (CH2)n?, wherein n? is an integer from 1 to 6, S is —SH, or —SS—, on the condition that free thiol (SH) of Cys is crosslinked by a disulfide bond to another Cys. The antigen determinant reacts sensitively and specifically with serum autoantibodies present in patients suffering from rheumatoid arthritis (RA) which is useful for diagnosing or treating RA.Type: GrantFiled: June 25, 2007Date of Patent: December 4, 2012Inventor: Yijun Xu
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Patent number: 8318162Abstract: The present disclosure relates to anti-high molecular weight melanoma associated antigen (HMW-MAA) antibodies which bind to human HMW-MAA. Such antibodies may be used to treat diseases or disorders characterized by expression of HMW-MAA including cancer, for example, melanoma, basal cell carcinoma, ALL or AML.Type: GrantFiled: July 16, 2010Date of Patent: November 27, 2012Assignee: Xoma Technology Ltd.Inventor: Arnold H. Horwitz
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Patent number: 8318915Abstract: The present invention relates to the detection and identification of different bacterial species, all of which cause zoonosis, based on DNA analysis. More specifically, the invention provides the primers, probes, genes and genic regions required to apply a method for the simultaneous detection of bacteria and bacterial groups belonging to the genera Anaplasma, Ehrlichia, Borrelia. Bartonella, Coxiella, Rickettsia and Francisella based on Multiple PCR analysis by RLB (Reverse Line Blotting), in addition to providing a kit to carry out said analysis.Type: GrantFiled: November 10, 2010Date of Patent: November 27, 2012Assignee: Instituto de Salud Carlos IIIInventors: Pedro Anda Fernandez, Raquel Escudero Nieto, Isabel Jado Garcia, Isabel Rodriguez Moreno, Maria Isabel Jiminez Alonso
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Patent number: 8309315Abstract: In a wide variety of human solid tumors, an aggressive, metastatic phenotype and poor clinical prognosis are associated with expression of the receptor tyrosine kinase Met. Disclosed herein are (a) a monoclonal antibody named Met4, which antibody is specific for Met, and (b) a hybridoma cell line that produces Met4. The Met4 antibody is particularly useful for detecting Met in formalin-fixed tissue. Methods of using the Met4 antibody for detection, diagnosis, prognosis, and evaluating therapeutic efficacy are provided.Type: GrantFiled: August 25, 2008Date of Patent: November 13, 2012Assignees: Van Andel Research Institute, Fred Hutchinson Cancer Research CenterInventors: Boliang Cao, George F. Vande Woude, Beatrice S. Knudsen, Ping Xu Zhao
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Patent number: 8298778Abstract: A method and a kit for detecting an antibody to Avibacterium paragallinarum are provided. A method for detecting an antibody to Avibacterium paragallinarum which comprises detecting an antibody induced by an outer-membrane protein of Avibacterium paragallinarum serotype A and/or serotype C by ELISA with a solid phase to which a peptide consisting of an amino acid sequence of non-homologous region of said outer-membrane protein or a portion thereof is immobilized, and a detection kit used for said method.Type: GrantFiled: February 6, 2009Date of Patent: October 30, 2012Assignee: The Chemo-Sero-Therapeutic Research InstituteInventors: Toshihiro Ushijima, Takashi Imamura, Ryuichi Sakamoto, Masashi Sakaguchi
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Patent number: 8283131Abstract: The present invention provides for the in vitro establishing of a prognosis for a subject in severe sepsis with at least two organ failures or in septic shock with at least two organ failures. Embodiments of the invention comprise the steps of measuring the level of the S100A8/A9 complex from a biological sample from a subject, and comparing the measured level to a predetermined threshold in which the measured level of the S100A8/A9 complex above the predetermined threshold is indicative of a bad prognosis and a measured level of the S100A8/A9 complex below the predetermined threshold is indicative of a good prognosis.Type: GrantFiled: October 28, 2008Date of Patent: October 9, 2012Assignee: Assistance Publique-Hopitaux de ParisInventors: Didier Payen De La Garanderie, Anne-Claire Lukaszewicz
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Patent number: 8278119Abstract: By detecting an antibody which immunologically reacts with amylase ?2-A in a sample, AIP or FT1DM is examined or the possibility of developing FT1DM is determined. For instance, detection of this antibody is carried out by an immunological method using an antigen which immunologically reacts with this antibody. The antigen is preferably a partial fragment containing the amino acid sequence of amino acid numbers 299 to 511 of human amylase ?2-A (SEQ ID NO: 1).Type: GrantFiled: May 2, 2008Date of Patent: October 2, 2012Assignees: University of Yamanashi, Cosmic CorporationInventors: Tetsuro Kobayashi, Toyoshi Endo, Atsushi Sakuraoka
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Patent number: 8268310Abstract: The invention concerns the prevention and treatment of complement-associated eye conditions, such as choroidal neovascularization (CNV) and age-related macular degeneration (AMD), by administration of Factor D antagonists.Type: GrantFiled: July 22, 2011Date of Patent: September 18, 2012Assignee: Genentech, Inc.Inventors: Philip Hass, Yin Jianping, Kenneth Katschke, Jr., Micah Steffek, Christian Wiesmann, Menno Van Lookeren Campagne
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Patent number: 8268316Abstract: Disclosed is a monoclonal antibody having very high affinity to anthrax toxin and potent toxin-neutralizing activity. Also disclosed are a composition for neutralizing anthrax toxin comprising the antibody and a kit for detecting anthrax toxin.Type: GrantFiled: March 10, 2006Date of Patent: September 18, 2012Assignee: Aprogen, IncInventors: Hyo Jeong Hong, Kyung Soo Inn, Nam Kyu Lim, Jung Whan Kim, Keun Soo Kim, Sang Yoon Lee, Se Yeon Kim, Hyun Jung Kang, Mee Sook Oh
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Patent number: 8247188Abstract: The disclosed invention relates to monoclonal antibodies (MAbs) which recognize human fatty acid synthase (hFAS) and are distinct from previously known anti-hFAS antibodies. Compositions, devices and kits comprising the MAbs are provided along with methods of using the MAbs in a variety of applications.Type: GrantFiled: August 17, 2006Date of Patent: August 21, 2012Assignee: Fasgen Diagnostics, LLCInventors: Susan Medghalchi, Jennifer L. Aldrich
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Patent number: 8246964Abstract: Antigenic protein fragments of Streptococcus pneumoniae to be used for the preparation of a medicament for the prevention and the treatment of bacterial infections and a method for the detection thereof, and related compositions using said epitopes, are disclosed.Type: GrantFiled: March 17, 2009Date of Patent: August 21, 2012Assignees: Universita' Degli Studi di SienaInventors: Concetta Beninati, Franco Felici, Marco Oggioni, Gianni Pozzi, Susanna Ricci
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Patent number: 8241857Abstract: To provide an immunological detection method which can detect or quantify a pneumococcal antigen in a sample derived from a living body conveniently, rapidly, and with high sensitivity, and an antibody for use in the method. The present invention provides an antibody which specifically recognizes a pneumococcal F-antigen; a method for detecting or quantitating a pneumococcal antigen, characterized in that the method detects or quantitates a pneumococcal F-antigen in a sample derived from a living body through immunological assay employing the antibody; and a kit for detecting a pneumococcal antigen, the kit containing the antibody.Type: GrantFiled: March 31, 2009Date of Patent: August 14, 2012Assignee: Otsuka Pharmaceutical Co., Ltd.Inventors: Suguru Akamatsu, Yoko Saijo
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Patent number: 8236519Abstract: In general, the present invention provides a reliable assay for the quantitative determination of p-aminophenol in an aqueous sample. More particularly, the present invention provides a rapid enzyme-based assay for the quantitative determination of acetaminophen in a sample. The assay employs a xylenol chromophore and a catalyst that is preferably a weak oxidizer. The assay provides reliable results in the presence or absence of N-acetylcystiene (NAC) and can therefore be used to monitor acetaminophen levels during NAC treatment. Methods and kits for determining acetaminophen concentration in an aqueous sample are also provided.Type: GrantFiled: April 17, 2009Date of Patent: August 7, 2012Assignee: Sekisui Diagnostics, LLCInventors: Michael Bulger, Jan Holinsky
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Patent number: 8231881Abstract: The present invention relates generally to a fusion protein made from a synthetic gene construct comprising of elements derived from the Leishmania antigens K26, K39, and K9. The fusion protein is particularly useful in the diagnosis of leishmaniasis, particularly visceral leishmaniasis in animals such as humans and dogs.Type: GrantFiled: July 2, 2009Date of Patent: July 31, 2012Assignee: Infectious Disease Research InstituteInventors: Ajay Bhatia, Steven G. Reed
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Patent number: 8222382Abstract: The present invention relates to the detection of HIV by amplification and PCR-based methods.Type: GrantFiled: June 3, 2005Date of Patent: July 17, 2012Assignee: Bio-Rad InnovationsInventors: Marie-Philippe Biron, Alain Horvais
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Patent number: 8222036Abstract: The present invention provides a method of diagnosing neoplasms having a particular phenotype by using oncolytic viruses that selectively replicate in neoplasms having the particular phenotype. For example, reovirus does not replicate in normal cells. However, reovirus selectively replicate in cells with an activated ras pathway, which leads to death of these cells. Therefore, a cell which becomes neoplastic due to, at least in part, elevated ras pathway activities can be diagnosed by its susceptibility to reovirus replication. This invention can further be applied, using other oncolytic viruses, to the diagnosis and/or treatment of other tumors, such as interferon-sensitive tumors, p53-deficient tumors and Rb-deficient tumors. Kits useful in the diagnosis or treatment disclosed herein are also provided.Type: GrantFiled: January 5, 2010Date of Patent: July 17, 2012Assignee: Oncoltyics Biotech Inc.Inventors: Bradley G. Thompson, Matthew C. Coffey
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Patent number: 8217150Abstract: According to the present invention, an antibody against a Panton-Valentine leukocidin toxin contained in Staphylococcus aureus, a method and a kit for detecting the toxin with the use of the antibody, and a pharmaceutical composition containing an antibody against a Panton-Valentine leukocidin toxin for treating PVL infection caused by Staphylococcus aureus containing PVL are provided. Also, an antibody which is capable of binding to Panton-Valentine leukocidin F and has no cross-reactivity to LukD and/or HlgB and an antibody which is capable of binding to Panton-Valentine leukocidin S and has no cross-reactivity to at least one of LukE, HlgC, and HlgA are provided.Type: GrantFiled: May 23, 2011Date of Patent: July 10, 2012Assignees: Juntendo University School of Medicine, Denka Seiken Co., Ltd.Inventors: Kanenari Ohishi, Tadashi Baba, Keiichi Hiramatsu, Yasuo Nakatomi
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Patent number: 8216795Abstract: A method of diagnosing in a host infection by or exposure to a mycobacterium which expresses ESAT-6 comprising (i) contacting a population of T cells from the host with one or more peptides or analogues selected from the peptides represented by SEQ ID NO:1 to 11 and analogues thereof which can bind a T cell receptor which recognizes any of the said peptides, and (ii) determining whether the T cells of said T cell population recognize the peptide(s) and/or analogue(s). The method may performed in vivo. Peptides and a kit which enable the method to be carried out are provided.Type: GrantFiled: January 25, 2011Date of Patent: July 10, 2012Assignee: Isis Innovation LimitedInventors: Ajit Lalvani, Ansar Pathan
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Patent number: 8192997Abstract: The invention provides methods for treatment of acute coronary syndrome and prediction of adverse cardiac events on the basis of elevations of catalytic iron in biological fluid of a human subject. An embodiment of the invention provides a method for early detection of acute coronary syndrome (ACS) in a human subject at the time of presentation of the chest pain. The method includes analyzing a test sample of the biological fluid for amount of catalytic iron and detecting acute coronary syndrome in the human subject.Type: GrantFiled: September 15, 2010Date of Patent: June 5, 2012Inventors: Mohan Rajapurkar, Suhas Lele, Sudhir Shah
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Patent number: RE44031Abstract: A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source.Type: GrantFiled: April 4, 2012Date of Patent: February 26, 2013Assignee: Battelle Energy Alliance, LLCInventors: William A. Apel, Vicki S. Thompson