Biospecific Ligand Binding Assay Patents (Class 436/501)
  • Patent number: 10717082
    Abstract: A device for sorting of rare target cells in a biological sample comprises a first row of at least two laminar-flow fluidic chambers, each comprising an inlet connected to a reservoir of a biological solution and an outlet and a last row of laminar-flow fluidic chambers equal in number to the number of chambers of the first row. Each last row fluidic chamber comprises an inlet connected to an outlet of a chamber of the first row and an outlet. A reservoir for collecting solution is connected to each outlet of the fluidic chambers of the second row, at least one pump causing a sample of the solution to circulate in the fluidic chambers of the first row then of the last row, wherein the fluidic chambers include a surface functionalized with molecules, at least some of which able to bond with a receptor molecule borne by the target cells.
    Type: Grant
    Filed: November 10, 2015
    Date of Patent: July 21, 2020
    Inventor: Phuong Lan Tran
  • Patent number: 10696900
    Abstract: A metal oxide/silicon dioxide-coated quantum dot and a preparation method thereof are provided. The metal oxide/silicon dioxide is selected from aluminum oxide/silicon dioxide, zirconium dioxide/silicon dioxide, titanium dioxide/silicon dioxide or zinc oxide/silicon dioxide, and the content of the metal oxide/silicon dioxide in the metal oxide/silicon dioxide-coated quantum dot is 1 wt % to 98 wt %. The metal oxide/silicon dioxide-coated quantum dot is prepared by one of a sol-gel method and a pyrolysis method.
    Type: Grant
    Filed: June 3, 2018
    Date of Patent: June 30, 2020
    Inventor: Liang Li
  • Patent number: 10697986
    Abstract: A method for optically reading information encoded in a microfluidic device, the microfluidic device including an input microchannel, microfluidic modules, and sets of nodes. Nodes of a first set connect the input microchannel to one of the microfluidic modules, and nodes of a second set connect the one of the microfluidic modules to another to form an ordered pair of the microfluidic modules, where the nodes of the first and second sets have different liquid pinning strengths. A liquid loaded into the input microchannel causes an ordered passage of the liquid through each of the microfluidic modules in an order determined by the liquid pinning strengths of the nodes. The passage of the liquid produces an optically readable dynamic pattern which evolves in accordance with the ordered passage of the liquid through the device.
    Type: Grant
    Filed: June 23, 2017
    Date of Patent: June 30, 2020
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Onur Gökçe
  • Patent number: 10683507
    Abstract: The subject invention provides methods, assays, and products for detecting small molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target, preferably, a synthetic cathinone in a sample comprises contacting the sample with an aptamer-based sensor selective for the small-molecule target, and detecting the small-molecule target in the sample. Specifically, the method utilizes an aptamer-based sensor comprising a dye binding to a three-way junction binding domain of an aptamer. Binding of small-molecule target to the aptamer displaces the dye, generating a spectroscopic signal that can be used for detection of the small-molecule target and quantitative measurement of the target concentration.
    Type: Grant
    Filed: February 20, 2018
    Date of Patent: June 16, 2020
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Yi Xiao, Haixiang Yu
  • Patent number: 10677953
    Abstract: A system for magnetic detection includes a magneto-optical defect center material including at least one magneto-optical defect center that emits an optical signal when excited by an excitation light; a radio frequency (RF) exciter system configured to provide RF excitation to the magneto-optical defect center material; an optical light source configured to direct the excitation light to the magneto-optical defect center material; and an optical detector configured to receive the optical signal emitted by the magneto-optical defect center material.
    Type: Grant
    Filed: May 31, 2017
    Date of Patent: June 9, 2020
    Assignee: LOCKHEED MARTIN CORPORATION
    Inventors: John B. Stetson, Arul Manickam, Peter G. Kaup, Gregory Scott Bruce, Wilbur Lew, Joseph W. Hahn, Nicholas Mauriello Luzod, Kenneth Michael Jackson, Jacob Louis Swett, Peter V. Bedworth, Steven W. Sinton, Duc Huynh, Michael John Dimario, Jay T. Hansen, Andrew Raymond Mandeville, Bryan Neal Fisk, Joseph A. Villani, Jon C. Russo, David Nelson Coar, Julie Lynne Miller, Anjaney Pramod Kottapalli, Gary Edward Montgomery, Margaret Miller Shaw, Stephen Sekelsky, James Michael Krause, Thomas J. Meyer
  • Patent number: 10655126
    Abstract: Methods and compounds useful to treat drug-induced diseases and conditions are provided. Methods of administering one or more MCJ-inhibiting compounds to decrease MCJ polypeptide activity in cells, tissues, and/or subjects as a treatment for a drug-induced disease or condition are provided.
    Type: Grant
    Filed: July 9, 2016
    Date of Patent: May 19, 2020
    Assignees: University of Vermont and State Agricultural College, Asociacion Centro de Investigacion Cooperativa en Biociencias-CIC bioGune
    Inventors: Mercedes Rincon, Maria Luz Martinez Chantar, Juan Anguita, Oliver Dienz
  • Patent number: 10612073
    Abstract: The present invention provides a method for constructing a single-stranded nucleic acid molecule for nucleic acid sequencing by means of a nanopore sequencer, said method including: a step in which at least one hairpin primer including a single-stranded region on the 3? side and a pair of primers are used to synthesize a complementary strand of template DNA that includes the target sequence; and a step in which the synthesized complementary strand forms a hairpin structure inside a molecule and a template extension reaction is carried out. The obtained nucleic acid molecule includes both the target sequence and the complementary strand thereof in the sequence. Single strand construction enables analysis by nanopore sequencing, and the sequence of only the target nucleic acid, which does not include information of the complementary strand, is repeatedly analyzed, thus enabling analysis to be conducted with greater precision by addressing the problem of sequence errors.
    Type: Grant
    Filed: February 26, 2016
    Date of Patent: April 7, 2020
    Assignee: Hitachi High-Technologies Corporation
    Inventor: Michiru Fujioka
  • Patent number: 10585102
    Abstract: The presently-disclosed subject matter includes labels for use in the identification of shed ALCAM in s sample from a subject. Further included are methods for characterizing, monitoring, evaluating treatment efficacy, or evaluating the progression of cancer in a subject that comprise providing a biological sample from the subject, determining the presence or amount of shed ALCAM in the biological sample, and then comparing the presence or the amount of the shed ALCAM to a reference. Differences between the presence or amount of shed ALCAM relative to the reference can be used to diagnose, prognosticate, treat, monitor, or otherwise characterize a cancer in a subject. Further provided are kits comprising a reagent and/or antibody for diagnosing, prognosticating, monitoring, or otherwise characterizing a cancer in a subject.
    Type: Grant
    Filed: October 11, 2013
    Date of Patent: March 10, 2020
    Assignee: Vanderbilt University
    Inventors: Andries Zijlstra, Amanda G. Hansen, Shanna Arnold
  • Patent number: 10550395
    Abstract: The subject invention provides methods, assays and products for detecting small-molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target in a sample comprises providing a sample, contacting the sample with an aptamer-based sensor selective for the small-molecule target, and sensitively and rapidly detecting the small-molecule target in the sample. Specifically, the method utilizes EATR-amplified small-molecule sensors based on cooperative binding split aptamers (CBSAs).
    Type: Grant
    Filed: April 6, 2018
    Date of Patent: February 4, 2020
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Yi Xiao, Haixiang Yu
  • Patent number: 10520515
    Abstract: Methods, devices, and reagents are described for performing assays for hemoglobin Ale, glycated albumin, and other glycated proteins. The methods involve a ratio determination between glycated protein and non-glycated protein. In some applications, the assay utilizes LOCI for signal generation. This invention is directed to assays and corresponding devices and reagents for detection of glycated protein, particularly including glycated hemoglobin. As is generally understood, such detection is useful in the management of blood glucose levels in diabetic patients and for monitoring the status of pre-diabetic individuals.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: December 31, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Victor Manneh, Sergei Svarovsky, Anthony Prestigiacomo
  • Patent number: 10501772
    Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: December 10, 2019
    Assignee: SeLux Diagnostics, Inc.
    Inventors: Eric Stern, Aleksandar Vacic, Benjamin Spears, Alec Flyer, Kelly Flentie
  • Patent number: 10422798
    Abstract: A problem of the present invention is to provide an immunochromatographic test strip avoiding agglutination of colloidal gold while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a hemagglutinating agent and the colloidal gold conjugates as a detection reagent, and to provide immunochromatography using the test strip. To solve the problem, the present inventors reviewed the composition of the existing reagent itself from a completely different viewpoint rather than the selection of type or amount of polyanions, and as a result of extensive study on each element, the inventors surprisingly found that agglutination of colloidal gold may be suppressed by using a particular additive without neutralization by polyanions.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: September 24, 2019
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Keigo Kohno, Mayumi Yoshida, Mitsuaki Yamamoto, Shinya Yokokawa, Marie Asami
  • Patent number: 10416109
    Abstract: Disclosed herein are processes and devices for use in the electrochemical detection of a target in a sample. For example, silicon or glass surfaces are treated with silanes functionalized with various side chains to tune the surface wetting characteristics.
    Type: Grant
    Filed: August 7, 2014
    Date of Patent: September 17, 2019
    Assignee: General Atomics
    Inventors: Graham D. Jack, Ryan B. Hayman
  • Patent number: 10408826
    Abstract: A chemical analysis apparatus that quantitatively determines an object of detection rapidly with high sensitivity, and a pretreatment apparatus and a chemical analysis method used for the chemical analysis apparatus, are provided. The chemical analysis apparatus includes a pretreatment unit that accommodates a molecularly imprinted polymer capable of capturing a polar group-containing molecule included in a specimen. A quantification unit quantitatively determines a component included in the specimen that has been passed through the pretreatment unit.
    Type: Grant
    Filed: March 15, 2017
    Date of Patent: September 10, 2019
    Assignee: HITACHI, LTD.
    Inventors: Shinichi Taniguchi, Takahiro Ando
  • Patent number: 10393701
    Abstract: Microfluidic methods of assaying molecule switching are provided. Aspects of the methods include microfluidically separating a sample containing the molecule of interest and then employing the resultant separation pattern to determine a switching characteristic of the molecule. Also provided are microfluidic devices, as well as systems and kits that include the devices, which find use in practicing embodiments of the methods. The methods, devices, systems and kits find use in a variety of different applications, such as analytical and diagnostic assays.
    Type: Grant
    Filed: September 24, 2013
    Date of Patent: August 27, 2019
    Assignee: The Regents of the University of California
    Inventors: Amy E. Herr, Augusto M. Tentori, Alex J. Hughes
  • Patent number: 10377797
    Abstract: The present invention relates to peptides, proteins, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer, in particular myeloma. The present invention furthermore relates to tumor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.
    Type: Grant
    Filed: November 20, 2018
    Date of Patent: August 13, 2019
    Assignee: IMMATICS BIOTECHNOLOGIES GMBH
    Inventors: Hans-Georg Rammensee, Juliane Stickel, Daniel Johannes Kowalewski, Stefan Stevanovic, Simon Walz
  • Patent number: 10358637
    Abstract: The carbohydrate processing enzyme PNGase F was catalytically inactivated through mutation. Additional mutations yielded a catalytically inactive carbohydrate-binding protein with lectin-like properties including high affinity and specificity N-linked glycans, O-linked glycans, or both.
    Type: Grant
    Filed: April 17, 2015
    Date of Patent: July 23, 2019
    Assignees: University of Georgia Research Foundation, Inc., Glycosensors and Diagnostics, LLC
    Inventors: Kausar N. Samli, Robert J. Woods, Loretta Yang
  • Patent number: 10309961
    Abstract: The present invention provides a bio-sensing device. The bio-sensing device includes an array of unit cells, each unit cell including: a source electrode and a drain electrode spaced apart from each other; a sensing film that serves as a channel between the source electrode and the drain electrode; and gate electrodes spaced apart from the sensing film, wherein the gate electrodes is disposed at a lower level than the source electrode, the drain electrode and the sensing film.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: June 4, 2019
    Assignee: NDD, INC.
    Inventors: Sae Young Ahn, Hyun Hwa Kwon
  • Patent number: 10296847
    Abstract: Presenting ancestral origin information, comprising: receiving a request to display ancestry data of an individual; obtaining ancestry composition information of the individual, the ancestry composition information including information pertaining to a proportion of the individual's genotype data that is deemed to correspond to a specific ancestry; and presenting the ancestry composition information to be displayed.
    Type: Grant
    Filed: June 13, 2016
    Date of Patent: May 21, 2019
    Assignee: 23andMe, Inc.
    Inventors: Chuong Do, Eric Durand, John Michael MacPherson, Brian Thomas Naughton, Joanna Louise Mountain
  • Patent number: 10252291
    Abstract: The present invention relates to a method for the preparation of a medical device element by means of extrusion or injection molding and to medical devices comprising such extruded or injection molded medical device elements. The medical device elements (e.g. tubes, wires, lines, stents, catheters, guides, endodontic instruments, needles, trocars for e.g. laparoscopic surgery, laparoscopic accessories, surgical instruments, guide wires) are characterized by a prefabricated shaped article or a thermoplastic substrate polymer having thereon a layer of a covalently cross-linked coating composition of a thermoplastic matrix polymer and a hydrophilic polymer. The method involves a coating composition comprising a thermoplastic matrix polymer, a hydrophilic polymer, and one or more photo-initiator(s), e.g. covalently linked to molecules of the thermoplastic matrix polymer and/or to molecules of the hydrophilic polymer.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: April 9, 2019
    Assignee: Coloplast A/S
    Inventors: Niels Jorgen Madsen, Egon Triel, Bo Rud Nielsen, Carsten Hoj
  • Patent number: 10222389
    Abstract: At least one fluoroalkyl surfactant and of at least one alcohol having 1 to 4 carbon atoms are used for dissociating vitamin D and/or a vitamin D metabolite from vitamin D binding protein. A solution comprising the at least one fluoroalkyl surfactant and at least one alcohol is provided. A method for detecting and quantifying, in vitro, vitamin D and/or at least one vitamin D metabolite in a biological sample includes treating the sample by incorporating at least one fluoroalkyl surfactant and at least one alcohol having 1 to 4 carbon atoms, so as to dissociate the vitamin D and/or its metabolite(s) to be detected from vitamin D binding protein; and detecting and quantifying vitamin D and/or at least one of its metabolites, in particular by immunoassay. A kit for detecting and quantifying vitamin D and/or at least one vitamin D metabolite by immunoassay and including the solution is provided.
    Type: Grant
    Filed: September 16, 2014
    Date of Patent: March 5, 2019
    Assignee: BIOMERIEUX
    Inventors: Valerie Meunier, Emmanuel Moreau
  • Patent number: 10159750
    Abstract: The invention described herein pertains to compounds and conjugates, to compositions, complexes and formulations comprising the compounds and/or conjugates, and to methods of use of the compounds, conjugates and their compositions, complexes and formulations in vaccines and vaccinations and generating immune responses.
    Type: Grant
    Filed: June 8, 2018
    Date of Patent: December 25, 2018
    Assignee: PURDUE RESEARCH FOUNDATION
    Inventors: Richard Frederic Borch, Irene A. George, Harm Hogenesch, Stanley L. Hem
  • Patent number: 10132959
    Abstract: Apparatus, methods, and systems related to a thin-layer spectroelectrochemistry cell; electrically coupling a second end of a working electrical wire lead, a second end of a counter electrical wire lead, and a second end of a reference electrical wire lead to a potentiostat; introducing a conductive fluid into a cell body in the spectroelectrochemistry cell; introducing a detection species into the cell body; introducing a sample into the cell body; applying a voltage potential across the transparent sample window to drive an electrochemical reaction between the detection species and the sample in the transparent sample window fluid; transmitting electromagnetic radiation into an optical path through the transparent sample window, thereby optically interacting the electromagnetic radiation with the transparent sample window fluid to generate modified electromagnetic radiation; receiving the modified electromagnetic radiation with a detector; and generating an output signal corresponding to a characteristic of
    Type: Grant
    Filed: February 20, 2015
    Date of Patent: November 20, 2018
    Assignee: Halliburton Energy Services, Inc.
    Inventors: Jing C. Shen, Dingding Chen, Michael T. Pelletier
  • Patent number: 10126308
    Abstract: Methods for quantifying biospecimen sample integrity using oxidation. Embodiments include assessing ex vivo oxidation of a biospecimen sample by quantifying a difference in protein oxidation of the biospecimen sample in comparison to protein oxidation of a portion of the biospecimen sample intentionally incubated to cause oxidation to hit its maximum value. The difference in a level of oxidation in the biospecimen sample and the portion of the biospecimen sample is then inversely proportional to the degree of ex vivo oxidation.
    Type: Grant
    Filed: March 27, 2017
    Date of Patent: November 13, 2018
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Chad Borges, Joshua Jeffs
  • Patent number: 10119933
    Abstract: The invention relates to a method for analysis by capillary electrophoresis of glycated haemoglobins comprising at least one globin chain comprising a glucose residue bound to the amino acid in the N-terminal position, contained in a biological sample, said method comprising using a buffer composition comprising at least one compound which is capable of specifically complexing glucose residues of one or several glycated haemoglobin(s) and of providing said glycated haemoglobin(s) with several negative electric charges at an alkaline pH. By way of example, this compound may be 3,4- or 3,5-dicarboxyphenylboronic acid, preferably 3,5-dicarboxyphenylboronic acid. Said method may in particular be used to separate and assay haemoglobin HbA1c present in a biological sample optionally comprising other haemoglobins, in particular other minor fractions.
    Type: Grant
    Filed: November 18, 2015
    Date of Patent: November 6, 2018
    Assignee: SEBIA
    Inventors: Gérald Deschamps, Frédéric Robert, Denis Simonin
  • Patent number: 10088478
    Abstract: Disclosed are nanostructured gold films which may be produced by solution-phase depositions of gold ions onto a variety of surfaces. The resulting plasmonic gold films are used for enhanced spectroscopic-based immunoassays in multiplexed microarray format with detection mechanisms based on either surface-enhanced Raman scattering or near-infrared fluorescence enhancement. The preparation of the films and subsequent modifications of the gold film surfaces afford increased sensitivity for various microarrays. The films are discontinuous, forming gold “islands.” Sensitivity, size, shape, and density of the nanoscopic gold islands comprising the discontinuous nanostructured gold film are controlled to enhance the intensity of Raman scattering and fluorescence in the near-infrared, allowing for improved measurements in clinical diagnostic or biomedical research applications.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: October 2, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Hongjie Dai, Scott M. Tabakman, Guosong Hong, Bo Zhang
  • Patent number: 10082474
    Abstract: The present invention is a method to quantify biomarkers. The method uses an X-ray fluorescence spectrometer to perform an X-ray fluorescence analysis on the sample to obtain spectral features derived from the biomarker; and quantifying the X-ray fluorescence signal of the biomarker.
    Type: Grant
    Filed: August 3, 2016
    Date of Patent: September 25, 2018
    Assignee: ICAGEN, INC.
    Inventors: Lori J. Peterson, Cameron A. Mortillaro, Benjamin P. Warner, Nathan H. Zahler, Pratima Bharti, Chang-Tai Hsieh, Emilia A. Solomon
  • Patent number: 10082501
    Abstract: Nanotube based lateral flow test device described herein is a lateral flow based diagnostic device, which uses arrays of fragments of single wall carbon nanotubes as sensor to detect the biomarkers at ultralow concentration (below picogram per milliliter). The device is consisted of the following components: (1). an lateral flow strip which typically is consisted of a backing film laminated with conjugate/sample pads, nitrocellulose membrane, wicking pad, (2). the arrays of single wall carbon nanotube conjugate with antibody which is immobilized on the membrane of the lateral flow device as the test line, (3). pairs of micro-electrode are installed on top part of the cassette, (4). a cassette which holds the lateral flow strip. The device can be used in clinical environments for biomarker detection.
    Type: Grant
    Filed: July 3, 2013
    Date of Patent: September 25, 2018
    Assignee: BioMedomics, Inc.
    Inventors: Xue-feng Wang, Qingqi Chen
  • Patent number: 10022424
    Abstract: The invention relates to the use of gelsolin to treat infections and to monitor the treatment of infections. The invention also provides methods up-regulating interleukin expression and methods for down-regulating pro-inflammatory cytokine expression.
    Type: Grant
    Filed: May 12, 2005
    Date of Patent: July 17, 2018
    Assignee: The Brigham and Women's Hospital, Inc.
    Inventors: Thomas P. Stossel, Po-Shun Lee
  • Patent number: 10010566
    Abstract: The invention provides methods and compositions useful for ameliorating age related maladies, enhancing and maintaining immune function, enhancing and maintaining cognitive function, enhancing and maintaining muscle strength, enhancing and maintaining balance, and reducing and mitigating oxidative stress in an animal. The compositions comprise a combination of at least two of one or more unsaturated fatty acids (UFA); one or more nitric oxide releasing compounds (NORC); one or more anti-glycation agents; and colostrum, and the methods comprise administering to an animal a therapeutically effective amount of the compositions.
    Type: Grant
    Filed: March 12, 2012
    Date of Patent: July 3, 2018
    Assignee: NestecSA
    Inventor: Ebenezer Satyaraj
  • Patent number: 10012631
    Abstract: The present invention is intended to provide a novel fluorescence sensor for target analysis, a kit for target analysis, and a target analysis method using the same. The fluorescence sensor for target analysis according to the present invention includes a nucleic acid molecule that includes a G-quartet-forming nucleic acid region (D) that forms a G-quartet and a binding nucleic acid region (A) that binds to a target. In the absence of a target, formation of a G-quartet in the G-quartet-forming nucleic acid region (D) is inhibited. In the presence of a target, the target comes into contact with the binding nucleic acid region (A), the G-quartet is formed in the G-quartet-forming nucleic acid region (D) due to the contact, the G-quartet-forming region (D) and porphyrin forms a complex, and the complex generates fluorescence.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: July 3, 2018
    Assignee: NEC Solution Innovators, Ltd.
    Inventors: Naoto Kaneko, Ikuo Shiratori, Katsunori Horii, Jou Akitomi, Iwao Waga
  • Patent number: 10006077
    Abstract: The invention is directed to compositions, e.g., cell-based and multiplexed platforms, to screen for small molecule drugs that inhibit enzymes such as proteases, e.g., viral proteases, e.g., HIV proteases; and methods for making and using these compositions. The invention provides compositions and methods for identifying compositions, e.g., drug molecules, that can inhibit proteases, e.g., viral proteases such as HIV proteases. In alternative embodiments, the invention provides cell-based platforms or assays to screen for compositions, e.g., small molecules or drugs, that inhibit or modify the activity of enzymes such as calcium-dependent protein convertases involved in HIV envelope protein processing, including cleavage of the HIV gp160 envelope precursor, resulting in gp120 and gp41 envelope products. In one embodiment, the invention provides a cell-based or multiplexed platform for monitoring the activity of enzymes, e.g., proteases such as viral proteases.
    Type: Grant
    Filed: May 2, 2017
    Date of Patent: June 26, 2018
    Assignee: San Diego State University (SDSU) Foundation
    Inventor: Roland Wolkowicz
  • Patent number: 9972086
    Abstract: Disclosed is an image quality evaluation method for a digital pathology system according to the present invention. The image quality evaluation method includes receiving a digital slide image by an image quality evaluation unit; dividing the digital slide image into a plurality of blocks by the image quality evaluation unit; analyzing the plurality of blocks to extract a foreground; calculating a blur for the extracted foreground; calculating brightness distortion for the extracted foreground; calculating contrast distortion for the extracted foreground; and evaluating the overall quality of the digital slide image using the blur, the brightness distortion, and the contrast distortion by the image quality evaluation unit.
    Type: Grant
    Filed: July 22, 2016
    Date of Patent: May 15, 2018
    Inventor: Duy Hien Pham
  • Patent number: 9957308
    Abstract: In order to provide a novel fluorescent protein and use thereof, the polypeptide according to the present invention has fluorescent properties in the presence of bilirubin and includes (1) the amino acid sequence of SEQ ID NO: 1, (2) an amino acid sequence having, for example, substitution of 1 to 21 amine acids in the amino acid sequence of SEQ ID NO: 1, (3) an amino acid sequence having 85% or more sequence identity to the amino acid sequence of SEQ ID NO: 1, or (4) the amino acid sequence encoded by a polynucleotide that hybridizes with a polynucleotide consisting of a sequence complementary to the polynucleotide encoding the polypeptide according to the amino acid sequence in (1) under a stringent condition.
    Type: Grant
    Filed: February 28, 2014
    Date of Patent: May 1, 2018
    Assignee: RIKEN
    Inventors: Atsushi Miyawaki, Akiko Kumagai
  • Patent number: 9943812
    Abstract: The present disclosure relates to a liquid filtration structure with one or more macromolecule membrane structures including membrane proteins selectively permeable to water molecules and fixed within a pore. A liquid filtration structure according to an exemplary embodiment of the present disclosure increases stability and durability of macromolecule membrane structures including membrane proteins selectively permeable to water molecules, and, thus, can be effectively used in a filtration device for purifying water.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: April 17, 2018
    Assignee: Inha-Industry Partnership Institute
    Inventors: Tae Joon Jeon, Sun Min Kim, Hyun II Ryu, Hui Soo Jang, Sun Hee Yoon, Young Ho Seo, Yong Min Park, Eui Don Han, Young Rok Kim, Yeong Eun Yoo, No Won Kim, Dae Joong Kim, Moon Ki Kim, Seung Hyun Kim, Jeong Hwan Kim, Jae Sung Yoon, Yun Jung Lee
  • Patent number: 9944976
    Abstract: A method of stirring a test substance-containing solution injected into an analysis chip, wherein said analysis chip includes a recess to which said test substance-containing solution is injected; and a selective binding substance, which selectively binds to said test substance, is immobilized on the entirety or a part of the bottom surface of said recess, said method including injecting said test substance-containing solution to the space in said recess of said analysis chip such that said space is partially left unfilled; and rotating said analysis chip to which said test substance-containing solution is injected applying a centrifugal acceleration of not less than 1×g.
    Type: Grant
    Filed: February 27, 2013
    Date of Patent: April 17, 2018
    Assignee: Toray Industries, Inc.
    Inventors: Toshihiko Kuroda, Hitoshi Nobumasa
  • Patent number: 9937256
    Abstract: In some aspects, methacrylate co-polymers crosslinked with an enzymatically cleavable peptide linker are provided and may be used for the oral delivery of a therapeutic. The peptide linker may be cleavable by an enzyme in the small intestine and may allow for the delivery of a therapeutic protein or nucleic acid to the small intestine. Also provided are methods of using the polymers for the treatment of a disease.
    Type: Grant
    Filed: January 13, 2016
    Date of Patent: April 10, 2018
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Jennifer M. Knipe, Laura E. Strong, Nicholas A. Peppas
  • Patent number: 9902904
    Abstract: Scale-inhibiting polymers comprising one or more scale-inhibiting units and one or more tagging units, wherein each tagging unit is formed from a compound of Formula I, II, III, or IV, or a salt, hydrate, salt hydrate, stereoisomer, dehydrate or derivative thereof. Scale-inhibiting compositions comprising the scale-inhibiting polymers, processes for determining a concentration of a scale-inhibiting polymer for inhibiting scale formation, and methods for preventing or controlling scale formation in systems comprising fluid circulation with the scale-inhibiting polymers are also disclosed herein.
    Type: Grant
    Filed: January 7, 2015
    Date of Patent: February 27, 2018
    Assignee: Kemira Oyj
    Inventors: Vesa Nuutinen, Susanna Toivonen
  • Patent number: 9897598
    Abstract: Techniques for enhanced fluorescence include a Tamm substrate for a target optical frequency comprising a metal nanoscale layer deposited on a Bragg grating. The Bragg grating includes multiple dielectric layers including multiple high index of refraction layers alternating with multiple low index of refraction layers. The dielectric layers are parallel to the metal nanoscale layer; and, the thickness of each dielectric layer is about a fourth of a wavelength of the target optical frequency in the layer. The metal nanoscale layer is configured to host a fluorophore such that an S polarized emission from the fluorophore at the target optical frequency propagates out of the substrate perpendicular to the plurality of dielectric layers.
    Type: Grant
    Filed: May 21, 2015
    Date of Patent: February 20, 2018
    Assignee: THE UNIVERSITY OF MARYLAND, BALTIMORE
    Inventors: Joseph R. Lakowicz, Ramachandram Badugu
  • Patent number: 9872886
    Abstract: Compounds comprising R-G-Cysteic Acid (i.e., R-G-NH—CH(CH2—SO3H)COOH or Arg-Gly-NH—CH(CH2—SO3H)COOH) and derivatives thereof, including pharmaceutically acceptable salts, hydrates, stereoisomers, multimers, cyclic forms, linear forms, drug-conjugates, pro-drugs and their derivatives. Also disclosed are methods for making and using such compounds including methods for inhibiting cellular adhesion to RGD binding sites or delivering other diagnostic or therapeutic agents to RGD binding sites in human or animal subjects.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: January 23, 2018
    Assignee: Allegro Pharmaceuticals, Inc.
    Inventors: Michael John Mackel, John Park
  • Patent number: 9862668
    Abstract: A monomer, a polymer, an organic layer composition, an organic layer and associated methods, the monomer being represented by Chemical Formula 1:
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: January 9, 2018
    Assignee: SAMSUNG SDI CO., LTD.
    Inventors: Youn-Hee Nam, Hyo-Young Kwon, Sung-Hwan Kim, Seung-Hyun Kim, Ran Namgung, Dominea Rathwell, Soo-Hyoun Mun, Seulgi Jeong, Hyeon-Il Jung, Yu-Mi Heo
  • Patent number: 9834808
    Abstract: The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.
    Type: Grant
    Filed: March 20, 2017
    Date of Patent: December 5, 2017
    Assignee: SeLux Diagnostics, Inc.
    Inventors: Eric Stern, Aleksandar Vacic, Benjamin Spears, Kelly Flentie, Alec Flyer
  • Patent number: 9810687
    Abstract: A nanoagent includes at least one nanocomposite. The nanocomposite includes at least one gold nanorod, a silver layer coated on an outer surface of the gold nanorod, a Raman reporter molecule layer coated on the silver layer, a pegylated layer coated on the Raman reporter molecule layer, an active layer conjugated to the pegylated layer. the active layer includes at least one of a targeting molecule configured to bind to a target of interest, and a functional molecule configured to interact with the target of interest. The silver layer has silver nanoparticles. The Raman reporter molecule layer has Raman reporter molecules that are detectable by surface enhanced Raman spectroscopy (SERS). The pegylated layer has at least one of thiolated polyethylene glycol (HS-PEG), thiolated polyethylene glycol acid (HS-PEG-COOH) and HS-PEG-NHx.
    Type: Grant
    Filed: April 10, 2015
    Date of Patent: November 7, 2017
    Assignee: BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS
    Inventors: Alexandru S. Biris, Andrew G. Kumpuris, Zeid Nima
  • Patent number: 9739765
    Abstract: The present invention provides a robust viability stain for methods utilizing elemental analysis. A population of cells is contacted with an effective dose of a non-chelated biomacromolecule-reactive metal derivative, which selectively crosses the plasma membrane of non-viable cells, and which covalently modifies a biological macromolecule within the cell, for a period of time sufficient to permit entry into non-viable cells. The population of cells is then washed free of unbound viability reagent; and the presence of the metal within the cells is detected, wherein non-viable cells are selectively labeled with the viability reagent.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: August 22, 2017
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Bernd Bodenmiller, Erin F. Simonds, Harris G. Fienberg, Garry P. Nolan
  • Patent number: 9726675
    Abstract: The invention relates to a novel diagnostic marker CT-proADM (C-terminal fragment of preproADM, SEQ ID No. 1) for diagnosing and/or stratifying the risk of diseases. Also disclosed is a method for diagnosing and/or stratifying the risk of diseases, particularly cardiovascular diseases, cardiac insufficiency, and infections and/or inflammations of the lungs and respiratory tract. In said method, the CT-proADM (SEQ ID No. 1) marker, or a partial peptide of fragment thereof, or said marker contained in a marker combination (panel, cluster) is determined in a patient who is to be examined. The invention further relates to a diagnostic apparatus as well as a kit for carrying out said method.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: August 8, 2017
    Assignee: B.R.A.H.M.S. GMBH
    Inventors: Andreas Bergmann, Joachim Struck
  • Patent number: 9714953
    Abstract: Methods, devices, and compositions for assaying therapeutic agents. In one aspect, methods, devices, and compositions for assaying paclitaxel to provide therapeutic drug monitoring guided therapy of paclitaxel.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: July 25, 2017
    Assignee: Autotelic LLC
    Inventors: Yueh Jung Lee, Chulho Park
  • Patent number: 9683254
    Abstract: The invention is directed to compositions, e.g., cell-based and multiplexed platforms, to screen for small molecule drugs that inhibit enzymes such as proteases, e.g., viral proteases, e.g., HIV proteases; and methods for making and using these compositions. The invention provides compositions and methods for identifying compositions, e.g., drug molecules, that can inhibit proteases, e.g., viral proteases such as HIV proteases. In alternative embodiments, the invention provides cell-based platforms or assays to screen for compositions, e.g., small molecules or drugs, that inhibit or modify the activity of enzymes such as calcium-dependent protein convertases involved in HIV envelope protein processing, including cleavage of the HIV gp160 envelope precursor, resulting in gp120 and gp41 envelope products. In one embodiment, the invention provides a cell-based or multiplexed platform for monitoring the activity of enzymes, e.g., proteases such as viral proteases.
    Type: Grant
    Filed: August 27, 2015
    Date of Patent: June 20, 2017
    Assignee: SAN DIEGO STATE UNIVERSITY (SDSU) FOUNDATION
    Inventor: Roland Wolkowicz
  • Patent number: 9658218
    Abstract: Methods and reagents are disclosed for determining a total amount of an analyte in an unknown sample suspected of containing the analyte in the presence of endogeneous interfering substances. The methods involve measuring an amount [Y] of the portion of the analyte in the unknown sample that is bound by endogeneous binding substances employing an assay for the analyte. An amount [Z] of analyte in the unknown sample that is not bound by endogeneous binding substances is determined by the formula: [Z]=a[Y]+b, wherein “a” and “b” are predetermined by conducting the assay on samples containing known amounts of the analyte but substantially free from endogeneous interfering substances. Adding [Y] and [Z] yields the total amount [X] of the analyte in the unknown sample.
    Type: Grant
    Filed: May 7, 2012
    Date of Patent: May 23, 2017
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Tie Quan Wei
  • Patent number: 9638705
    Abstract: Described herein are methods for determining the overall survival of maintenance hemodialysis patients. The methods include measuring low density lipoprotein (LDL) particle size and subfraction concentrations as prognostic tools for early mortality risk detection. For example, the presence of increased very small LDL concentration or decreased LDL particle size in blood-serum serves as a useful means for prognostic risk assessment and monitoring.
    Type: Grant
    Filed: October 27, 2011
    Date of Patent: May 2, 2017
    Assignees: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED, LOS ANGELES BIOMEDICAL RESEARCH INSTITUTE
    Inventors: Kamyar Kalantar-Zadeh, Michael P. Caulfield, Wael A. Salameh
  • Patent number: 9618508
    Abstract: The invention utilizes the techniques and instruments which are typically used in flow cytometry (FC) as a tool for analyzing adsorbed materials. Thus the invention provides a method for analyzing a component which is adsorbed to an insoluble metal salt, comprising steps of: (i) labelling the adsorbed component with a binding reagent; and (ii) analyzing the labelled adsorbed component by flow cytometry.
    Type: Grant
    Filed: December 14, 2011
    Date of Patent: April 11, 2017
    Assignee: GlaxoSmithKline Biologicals SA
    Inventors: David Skibinski, Donatello Laera, Sandra Nuti, Mildred Ugozzoli