Lipids, Triglycerides, Cholesterol, Or Lipoproteins Patents (Class 436/71)
  • Patent number: 10434492
    Abstract: A composition, method and device for the preparation of biological samples for subsequent instrumental analyses, such as GC, GC-MS, LC and LC-MS analysis, using a solid phase extraction (SPE) process is described. Through SPE process alone or an integrated combination of protein precipitation, filtration, and SPE using a hydrophobic zirconia-coated chromatographic media, interfering compounds, such as proteins, glycerides and phosphate-containing compounds, are eliminated from the biological, food, environmental and biotechnology samples, affording an enhanced analyte response during the instrumental analysis.
    Type: Grant
    Filed: May 21, 2010
    Date of Patent: October 8, 2019
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Xiaoning Lu, Olga I. Shimelis, Maochun M. Ye
  • Patent number: 10345318
    Abstract: A method is described for the diagnostic determination of the risk caused by an altered oxidative balance, comprising the photometric measurement of total cholesterol, hydroperoxides, and antioxidant capacity, on the basis of which the protective index, the oxidative index and the risk index caused by an altered oxidative balance (or ‘OBRI’) are calculated. This latter index has proved to be particularly dependable and reliable in determining the status of the oxidative balance in relation to cholesterol levels, being highly predictive of cardiovascular risk.
    Type: Grant
    Filed: March 31, 2015
    Date of Patent: July 9, 2019
    Assignee: H&D S.R.L.
    Inventor: Gabriele Callegari
  • Patent number: 10221453
    Abstract: The present invention relates to methods, monitors and systems, useful, for example, for advanced detection of sepsis in a subject.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: March 5, 2019
    Assignee: Becton, Dickinson and Company
    Inventors: Song Shi, Richard L. Moore, James Garrett
  • Patent number: 9952231
    Abstract: Embodiments of methods and compounds for isolating and detecting lysophosphatidic acids (LPAs) are disclosed. Kits for performing the methods also are disclosed. LPAs are isolated from biological samples by liquid-liquid extraction followed by solid phase extraction. LPA species may be separated by HPLC, and the separated species may be identified and quantified. Also disclosed are embodiments of compounds capable of universally detecting a plurality of LPA species with substantially equivalent sensitivity. Embodiments of the disclosed compounds are useful for determination of total LPA concentration in a sample comprising a plurality of LPA species without separation of individual LPA species.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: April 24, 2018
    Assignee: Portland State University
    Inventors: Robert Strongin, Martha Sibrian-Vazquez, Jialu Wang, Lei Wang, Jorge O. Escobedo-Cordova
  • Patent number: 9890412
    Abstract: Provided is a method for separately or simultaneously quantifying whole HDL-C and cholesterol in HDL subfractions: ApoE-Containing HDL-C and ApoE-deficient HDL-C. A method for enzymatically and separately quantifying cholesterol in the ApoE-deficient HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative to a test sample in a final concentration of 0.05 to 0.10%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated. A method for enzymatically and separately quantifying cholesterol in ApoE-Containing HDL comprising: adding a surfactant composed of a polyoxyethylene benzyl phenyl ether derivative so as to obtain a final concentration of 0.15 to 0.75%, reacting the test sample with cholesterol esterase and cholesterol oxidase, and quantifying hydrogen peroxide generated.
    Type: Grant
    Filed: April 10, 2013
    Date of Patent: February 13, 2018
    Assignees: Denka Seiken Co., Ltd., National University Corporation Hokkaido University
    Inventors: Yasuki Itoh, Hitoshi Chiba
  • Patent number: 9861685
    Abstract: Methods of alleviating the symptoms of hemoglobinopathies, including, but not limited to, sickle cell disease, ?-thalassemia, and hemoglobin H disease are provided. In some embodiments, the methods comprise administering an inhibitor selected from a ?-arrestin1/2 inhibitor and/or a GRK2 inhibitor to the subject. Methods of inhibiting adhesion of sickle red blood cells to endothelial cells and adhesion to and activation of leukocytes are also provided.
    Type: Grant
    Filed: November 20, 2013
    Date of Patent: January 9, 2018
    Assignee: Duke University
    Inventor: Rahima Zennadi
  • Patent number: 9642875
    Abstract: The invention provides a propionate inulin ester for the reduction of appetite, food intake and/or calorie intake and/or to improve insulin sensitivity in a subject, and for the treatment or prevention of obesity or diabetes. The invention also provides compositions comprising a propionate inulin ester, methods using propionate inulin esters, functional food containing propionate inulin ester and methods of making propionate inulin esters.
    Type: Grant
    Filed: July 31, 2013
    Date of Patent: May 9, 2017
    Assignees: The University Court of the University of Glasgow, Imperial Innovations Limited
    Inventors: Gary Frost, Douglas Morrison, Thomas Preston
  • Patent number: 9594891
    Abstract: (Purpose) The present invention provides a technology capable of simply performing individual authentication with high accuracy by extracting the feature of the vein pattern and the feature of the palm print shape of the person to be authenticated from a single original image data photographed using a visible light image acquisition unit (e.g. visible light camera). (Solution) An authentication light source 11 irradiates light which contains at least red light in the visible light region toward a palm of human body. An authentication image acquisition unit 12 acquires at least one reflection image to form by the light which is irradiated from the authentication light source 11, and is reflected on the palm of human body. An authentication image processing unit 13 performs image processing of the reflected image, and extracts the shape of palm print and the shape of veins in the palm respectively from a single sheet of the reflected image for the authentication to generate the authentication data.
    Type: Grant
    Filed: August 27, 2012
    Date of Patent: March 14, 2017
    Assignee: Universal Robot Kabushiki Kaisha
    Inventor: Eizaburo Iwata
  • Patent number: 9575079
    Abstract: Disclosed are an apparatus and a method for effectively measuring cholesterol using a small amount of blood. The apparatus for measuring cholesterol includes a cartridge where an upper case having a sample transferring membrane is movably coupled to a lower case having a sample measuring membrane; a cartridge accommodation unit configured to accommodate the cartridge therein; a memory configured to store therein setting information for sample measurement; and a controller configured to align the membranes of the upper case and the lower case with each other, by horizontally moving the lower case of the cartridge, according to the stored setting information, and configured to contact the membranes of the upper case and the lower case with each other, by vertically moving the upper case for sample transfer on each alignment position.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: February 21, 2017
    Assignee: LG ELECTRONICS INC.
    Inventors: Jisu Kim, Seokjung Hyun, Yongju Yang, Kangsun Lee, Hyunho Oh, Guhan Kwon, Younjae Lee, Bongchu Shim, Gyoungsoo Kim, Dayeon Kang, Yonghyun Kim
  • Patent number: 9568454
    Abstract: The invention provides novel, simple, reliable and effective methods for separating various lipids from biological samples. In certain embodiments, the invention provides HILIC-based UHPLC separation methods. In one embodiment, the methods of the invention offer an efficient and rapid separation of ether glycophospholipids (e.g., plasmalogens) from their diacyl or monoacyl counterparts within the same lipid class. In certain instances, the invention relates to the use of a Waters ACQUITY UPLC® system.
    Type: Grant
    Filed: April 11, 2014
    Date of Patent: February 14, 2017
    Assignee: Waters Technologies Corporation
    Inventors: Stephen Wong, Mark Ritchie
  • Patent number: 9435870
    Abstract: Methods, computer program products and apparatus determine a subject's risk of having or developing CHD using a calculated HDL particle risk number and/or a mathematical model of risk associated with HDL particles that adjusts concentrations of at least one of the subclasses of small, medium and large HDL particle measurements to reflect predicted CHD risk. A calculated LDL particle risk number may also be generated as well as a lipoprotein particle index derived from the ratio of RLDL/RHDL.
    Type: Grant
    Filed: May 10, 2007
    Date of Patent: September 6, 2016
    Assignee: LIPOSCIENCE, INC.
    Inventor: James D. Otvos
  • Patent number: 9304136
    Abstract: The present invention pertains to the field of toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing increased peroxisomal proliferation. It also relates to a method of determining whether a compound is capable of inducing such peroxisomal proliferation in a subject and to a method of identifying a drug for treating increased peroxisomal proliferation. Furthermore, the present invention relates to a data collection including characteristic values of at least five metabolites, a data storage medium including data collection, and a system and a device for diagnosing increased peroxisomal proliferation. Finally, the present invention pertains to the use of a group of metabolites or means for the determination thereof for the manufacture of a diagnostic device or composition for diagnosing increased peroxisomal proliferation in a subject.
    Type: Grant
    Filed: January 10, 2014
    Date of Patent: April 5, 2016
    Assignee: BASF SE
    Inventors: Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Tilmann B. Walk, Ralf Looser, Edgar Leibold, Hennicke Kamp, Georgia Coelho Palermo Cunha, Michael Manfred Herold, Jan C. Wiemer, Alexandre Prokoudine
  • Patent number: 9274126
    Abstract: Biomarkers and methods are disclosed for diagnosing the risk of a myocardial infarction in an individual by measuring the levels of a set of biomarkers in a sample from an individual. A risk score is calculated for the individual by weighting the measured levels of the biomarkers. The risk score then is used to identify whether the individual is likely to experience a myocardial infarction. In addition, kits are disclosed that include a set of reagents for specifically measuring biomarker levels in a sample from an individual.
    Type: Grant
    Filed: November 15, 2010
    Date of Patent: March 1, 2016
    Assignee: BG Medicine, Inc.
    Inventors: Aram S. Adourian, Yu Guo, Xiaohong Li, Pieter Muntendam
  • Patent number: 9228211
    Abstract: A process of water degumming an edible oil (preferably a crude edible oil) comprising the steps of: a) admixing approximately 0.1-5% w/w water with an edible oil (preferably a crude edible oil) and a lipid acyltransferase, b) agitating the admixture for between about 10 minutes and 180 minutes at about 45 to about 900 C, and c) separating the oil phase and the gum phase. Preferably said lipid acyltransferase is a polypeptide having lipid acyltransferase activity which polypeptide is obtained by expression of the nucleotide sequence shown as SEQ ID No. 49 or a nucleotide sequence which as has 70% or more identity therewith; and/or is obtained by expression of a nucleic acid which hybridizes under medium stringency conditions to a nucleic probe comprising the nucleotide sequence shown as SEQ ID No. 49; and/or is a polypeptide having lipid acyltransferase activity which polypeptide comprises the amino acid sequence shown as SEQ ID No. 68 or an amino acid sequence which as has 70% or more identity therewith.
    Type: Grant
    Filed: December 11, 2008
    Date of Patent: January 5, 2016
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Jørn Borch Søe, Anne Victoria Brown
  • Patent number: 9200322
    Abstract: The present invention provides methods and compositions for the diagnosis of acute ischemic stroke. The invention further provides methods and compositions for distinguishing acute ischemic stroke from other forms of stroke and TIAs and “stroke mimic” events. Moreover, methods and compositions are provided to facilitate the treatment of acute ischemic stroke patients.
    Type: Grant
    Filed: February 22, 2011
    Date of Patent: December 1, 2015
    Assignees: The United States of America, as Represented by the Secretary, Department of Health and Human Services, University of Pittsburgh—of the Commonwealth System of Higher Education
    Inventors: Taura L. Barr, Maria Del Mar Matarin, Steven Jay Warach, Andrew Barry Singleton, Yvette P. Conley
  • Patent number: 9164085
    Abstract: Subject of the invention is a test strip for the detection of equol by a color change. Subject of the invention are also methods for producing the test strip, methods for using the test strip, and uses of the test strip.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: October 20, 2015
    Assignee: System Biologie AG
    Inventor: Juerg Daniel Schmid
  • Patent number: 9122777
    Abstract: Markers and methods useful for assessing coronary artery disease in a subject are provided, along with kits for measuring their expression. Also provided are predictive models, based on the markers, as well as computer systems, and software embodiments of the models for scoring and optionally classifying samples.
    Type: Grant
    Filed: June 15, 2010
    Date of Patent: September 1, 2015
    Assignee: CardioDx, Inc.
    Inventors: Steven Rosenberg, Michael R. Elashoff, Philip Beineke, James A. Wingrove, Whittemore G. Tingley, Susan E. Daniels
  • Publication number: 20150147817
    Abstract: The present invention generally relates to the field of biomarkers. In particular, the present invention relates to biomarkers such as PC-O 44:4 that can be used, for example for detecting and/or quantifying visceral adiposity and/or changes in visceral adiposity. This biomarker may also be used to diagnosing the effect of a change in lifestyle on visceral adiposity in a subject.
    Type: Application
    Filed: November 25, 2014
    Publication date: May 28, 2015
    Inventors: Francois-Pierre Martin, Ivan Montoliu Roura, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Publication number: 20150132742
    Abstract: Microfluidic devices fabricated from paper that has been covalently modified to increase its hydrophobicity, as well as methods of making and using thereof are provided herein. The devices are typically small, portable, flexible, and both easy and inexpensive to fabricate. Microfluidic devices contain a network of microfluidic components, including microfluidic channels, microfluidic chambers, microwells, or combinations thereof, designed to carry, store, mix, react, and/or analyze liquid samples. The microfluidic channels may be open channels, closed channels, or combinations thereof. The microfluidic devices may be used to detect and/or quantify an analyte, such as a small molecules, proteins, lipids polysaccharides, nucleic acids, prokaryotic cells, eukaryotic cells, particles, viruses, metal ions, and combinations thereof.
    Type: Application
    Filed: June 3, 2013
    Publication date: May 14, 2015
    Inventors: Martin Mwangi Thuo, Ramses V. Martinez, Ana C. Glavan, Wenjie Lan, Xinyu Liu, Jean-Francis Bloch, George M. Whitesides
  • Publication number: 20150132746
    Abstract: Provided herein is technology relating to the collection of biological samples and particularly, but not exclusively, to compositions, methods, and uses related to using a biopolymer substrate to collect biological samples for analysis.
    Type: Application
    Filed: May 29, 2013
    Publication date: May 14, 2015
    Inventors: Astrid Gjelstad, Lars Erik Eng Eibak, Anne Bee Hegge, Knut Einar Rasmussen, Stig Pedersen-Bjergaard
  • Patent number: 9029162
    Abstract: Disclosed are methods and systems for the analysis of testosterone in a sample using supported liquid extraction and liquid chromatography-mass spectrometry.
    Type: Grant
    Filed: February 7, 2012
    Date of Patent: May 12, 2015
    Assignee: Laboratory Corporation of America Holdings
    Inventors: Russell Philip Grant, Matthew Crawford, Donald Walt Chandler, William Curtin
  • Publication number: 20150111199
    Abstract: Methods of studying, interrogating, analyzing, and detecting particles, substances, and the like with near field light are described. Methods of identifying binding partners, modulators, inhibitors, and the like of particles, substances, and the like with near field light are described. In certain embodiments, the methods comprise immobilizing or trapping the particle, substance, and the like.
    Type: Application
    Filed: March 15, 2013
    Publication date: April 23, 2015
    Inventors: Robert Hart, Bernardo Cordovez
  • Publication number: 20150111234
    Abstract: A portable, hand-held glucose testing device includes a housing configured to accommodate a plurality of test sensors in a stacked arrangement and having a wall with an opening defined therein. A plurality of packaged test sensors is stacked in alignment with one another within the housing. Each of the test sensors is packaged within a blister package. The blister package includes a blister package housing and a cover foil overlying a surface of the blister package housing and the test sensor. A drive slide is configured to displace one of the plurality of packaged test sensors out of alignment with other packaged test sensors. A knife mechanism is configured to pierce through the cover foil, and to engage and urge the test sensor to extend through the opening for receiving a sample. A meter contact is configured to engage the test sensor when the test sensor extends through the opening.
    Type: Application
    Filed: December 23, 2014
    Publication date: April 23, 2015
    Inventors: Steven C. Charlton, Allen Brenneman
  • Patent number: 9012162
    Abstract: The inventors have proposed a novel panel of human serum protein biomarkers for diagnosing hepatic fibrosis and cirrhosis. Presently there is no reliable non-invasive way of assessing liver fibrosis. A 2D-PAGE based proteomics study was used to identify potential fibrosis biomarkers. Serum from patients with varying degrees of hepatic scarring induced by infection with the hepatitis C virus (HCV) was analyzed. Several proteins associated with liver scarring and/or viral infection were identified. These proteins include the inter-?-trypsin inhibitor heavy chain H4 fragments, complement factor H-related protein 1, CD5L, Apo L1, and ?2GPI. Increased and decreased thiolester cleavage of a2M and Complement C3, respectively, was also detected. The concentrations of these novel biomarkers can be determined using an immunoassay where the concentrations would reflect the extent of fibrosis. A fibrosis scoring scale for each of the novel biomarkers is proposed.
    Type: Grant
    Filed: September 7, 2007
    Date of Patent: April 21, 2015
    Assignee: The Chancellor, Masters and Scholars of The University of Oxford
    Inventors: Bevin Gangadharan, Nicole Zitzmann, Raymond A. Dwek
  • Publication number: 20150105296
    Abstract: Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort compromising centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using hydroxy-sphingomyelin SM-OH-22:1 as biomarker.
    Type: Application
    Filed: September 19, 2014
    Publication date: April 16, 2015
    Inventors: Sebastiano Collino, Ivan Montoliu Roura, Francois-Pierre Martin, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Publication number: 20150104877
    Abstract: A reusable coating for a nanopore structure is disclosed herein. A nanopore structure includes a substrate comprising a nanochannel and a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel. The chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel. Methods for making and using the reusable coating are also disclosed.
    Type: Application
    Filed: November 4, 2013
    Publication date: April 16, 2015
    Applicant: International Business Machines Corporation
    Inventors: Natalie Gunn, Jose M. Lobez Comeras, Priscilla Rogers, John Wagner
  • Publication number: 20150099726
    Abstract: This invention provides a dry powder inhaler comprising: a dry powder medicament comprising fluticasone propionate, salmeterol xinafoate and a lactose carrier; wherein, the delivered dose of salmeterol per actuation is less than 50 ?g; and wherein the dose provides a baseline-adjusted FEV1 in a patient of more than 150 mL within 30 minutes of receiving the dose. A method of treating a patient includes administering to a patient a dry powder medicament having fluticasone propionate, salmeterol xinafoate and a lactose carrier; wherein, the delivered dose of salmeterol per actuation is less than 50 ?g; and wherein the dose provides a baseline-adjusted FEV1 in a patient of more than 150 mL within 30 minutes of receiving the dose.
    Type: Application
    Filed: October 6, 2014
    Publication date: April 9, 2015
    Applicant: Teva Branded Pharmaceutical Products R&D, Inc.
    Inventors: Mukul DALVI, Seah Kee TEE
  • Patent number: 8999721
    Abstract: Provided is a method for assessing cardiovascular risk in Metabolic Syndrome and Type 2 Diabetes patients. The method involves obtaining data from a Type 2 diabetes patient or a Metabolic Syndrome patient and determining a Fayad/Schentag index which includes a Glucose Supply Index (S) to an Insulin Demand Index (D) ratio. The Fayad/Schentag index is used in scoring cardiovascular risks of Metabolic Syndrome patients and for recommending and implementing therapeutic interventions that can be shown to lower cardiovascular risk.
    Type: Grant
    Filed: January 30, 2013
    Date of Patent: April 7, 2015
    Assignee: Therabrake, Inc.
    Inventors: Jerome J. Schentag, Joseph M. Fayad, Scott V. Monte
  • Patent number: 8999716
    Abstract: Provided herein are artificial membranes of mycolic acids. The membranes may be unsupported or tethered. These membranes are long lived and highly resistant to electroporation, demonstrating their general strength. The mycolic acid membranes are suitable for controlled studies of the mycobacterial outer membrane and can be used in other experiments, such as nanopore analyte translocation experiments.
    Type: Grant
    Filed: August 22, 2012
    Date of Patent: April 7, 2015
    Assignee: University of Washington
    Inventors: Jens Gundlach, Ian M. Derrington, Kyle W. Langford
  • Publication number: 20150093771
    Abstract: An analytical device for analysis of chemical or biological samples, a method of using such a device, based on rotation of the device, integrated sample dosing and optical detection, and a system comprising such a device are disclosed. The analytical device comprises a device body having a liquid processing unit. The liquid processing unit comprises a mixing chamber for mixing a sample with a reagent, a sample dosing chamber for delivering a defined volume of the sample to the mixing chamber, and a reagent channel for delivering the reagent to be mixed with the sample, wherein the mixing chamber also serves as a detection chamber.
    Type: Application
    Filed: December 2, 2014
    Publication date: April 2, 2015
    Inventors: Patrick Griss, Rainer Jaeggi, Goran Savatic, Vuk Siljegovic
  • Publication number: 20150080264
    Abstract: The present invention generally relates to the field of biomarkers. In particular, the present invention relates to biomarkers such as PC-O 44:6 that can be used, for example for detecting and/or quantifying visceral adiposity and/or changes in visceral adiposity. This biomarker may also be used to diagnosing the effect of a change in lifestyle on visceral adiposity in a subject.
    Type: Application
    Filed: November 25, 2014
    Publication date: March 19, 2015
    Inventors: Francois-Pierre Martin, Ivan Montoliu Roura, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Publication number: 20150072043
    Abstract: Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort compromising centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using 9-oxo-HODE as biomarker.
    Type: Application
    Filed: September 19, 2014
    Publication date: March 12, 2015
    Inventors: Sebastiano Collino, Ivan Montoliu Roura, Francois-Pierre Martin, Philippe Alexandre Guy, Serge Andre Dominique Rezzi
  • Publication number: 20150072366
    Abstract: The present invention provides methods for continuous measurement of triglyceride digestibility during a meal. A stable isotope labeled triglyceride and a free fatty acid tracer are added to the meal. The ratio between a ratio of isotope labeled fatty acid produced after digestion to free fatty acid tracer represents the percentage of digestion of triglycerides by lipase from the pancreas.
    Type: Application
    Filed: November 17, 2014
    Publication date: March 12, 2015
    Inventors: Nicolaas E. Deutz, Marielle P. Engelen, Guinur Com, John J. Thaden
  • Publication number: 20150065460
    Abstract: Biomarkers relating to pancreatic Beta-cell function, pancreatic Beta-cell glucose sensitivity, insulin resistance, and/or pancreatic Beta-cell-related disorders are provided. Methods based on the same biomarkers are also provided.
    Type: Application
    Filed: September 11, 2012
    Publication date: March 5, 2015
    Inventors: Walter Gall, Kay A. Lawton
  • Patent number: 8969088
    Abstract: A substrate mimicking intercellular lipids in stratum corneum consisting of a substrate and a lipid membrane formed on the substrate, wherein the lipid membrane is formed from ceramide, palmitic acid and cholesterol, and the ceramide, palmitic acid and cholesterol are present at a mass ratio of 20-70%:10-60%:20-40% (ceramide:palmitic acid:cholesterol).
    Type: Grant
    Filed: September 1, 2009
    Date of Patent: March 3, 2015
    Assignee: Shiseido Company, Ltd.
    Inventors: Takuya Saiwaki, Takashi Oka, Yuichiro Mori, Toyoko Imae, Xiaojuan Wang, Masaki Ujihara
  • Publication number: 20150056708
    Abstract: Provided are methods for determining the amount of estrone in a sample using mass spectrometry. The methods generally involve ionizing estrone in a sample and detecting and quantifying the amount of the ion to determine the amount of estrone in the sample.
    Type: Application
    Filed: November 5, 2014
    Publication date: February 26, 2015
    Inventors: Mildred M. GOLDMAN, Richard E. REITZ
  • Publication number: 20150051111
    Abstract: A method is described for mass spectrometric analysis of a sample comprising phenolic OH, such as a steroid comprising a phenolic OH, using a quaternary amino oxy Cookson (QAOC) reagent. The QAOC reagent can improve ionization and fragmentation properties of phenolic OH samples, which can thereby improve quantitation and identification. The method can include derivatizing the phenolic OH sample with the QAOC reagent to create an adduct and analyzing the adduct using mass spectrometry. Derivatization of the sample can be a one-step reaction where the QAOC reagent comprises an aminooxy MS tag or can be a multi-step reaction, where the adduct is formed by the reaction of carbonyl substituted PTAD based reagent and the sample followed by combination with an aminooxy MS tag. The sample can also be enriched prior to reacting it with the reagent. The method can also allow for multiplexing.
    Type: Application
    Filed: January 16, 2013
    Publication date: February 19, 2015
    Inventors: Subhakar Dey, Subhasish Purkayastha
  • Publication number: 20150044775
    Abstract: For checking engine oil, especially containing rate of FAME (Fatty Acid Methyl Ester), we provide a measuring device including a cylindrical and upright analysis vessel having a reading scale with which a surface boundary is measured. In the vessel, the engine oil is mixed with reagents including alcohol(s) and a demulsifier. And the decreased volume is checked as volume of the FAME contained in the engine oil.
    Type: Application
    Filed: January 22, 2013
    Publication date: February 12, 2015
    Applicant: YANMAR Co., LTD
    Inventors: Kay Kian Hee, Kamaruddin Kudumpor, Beverly Kah Waynee, Siti Norbaria Sally Bt. Pick Thall Tan, Aaron Willinton William
  • Publication number: 20150044774
    Abstract: Disclosed is the provision of a method for quantifying HDL3 in a test sample without requiring a laborious operation. The method for quantifying cholesterol in high-density lipoprotein 3 comprises allowing a surfactant(s) which specifically react(s) with a high-density lipoprotein 3 to react with a test sample and quantifying cholesterol, and the surfactant(s) is(are) at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ether and polyoxyethylene styrenated phenyl ether.
    Type: Application
    Filed: January 24, 2013
    Publication date: February 12, 2015
    Applicant: DENKA SEIKEN CO., LTD
    Inventors: Noriyuki Sato, Yuhko Hirao, Yasuki Itoh
  • Patent number: 8945930
    Abstract: A device adapted to determine an analyte concentration of a fluid sample using a test sensor. The device comprises a display adapted to display information to a user. The device further comprises at least one user-interface mechanism adapted to allow the user to interact with the device. The device further comprises a body portion including at least one opening formed therein, the at least one opening being of sufficient size to receive the test sensor. The device further comprises a memory adapted to store a plurality of stored analyte concentrations. The device further comprises a processing feature adapted to inhibit the stored analyte concentrations from being displayed on the display.
    Type: Grant
    Filed: February 6, 2014
    Date of Patent: February 3, 2015
    Assignee: Bayer HealthCare LLC
    Inventor: Kevin Chang
  • Publication number: 20150031572
    Abstract: The present invention pertains to the field of diagnostics for neuronal toxicity and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing neuronal toxicity. It also relates to a method for determining whether a compound is capable of inducing such neuronal toxicity in a subject and to a method of identifying a drug for treating neuronal toxicity. Furthermore, the present invention relates to a device and a kit for diagnosing neuronal toxicity.
    Type: Application
    Filed: February 15, 2013
    Publication date: January 29, 2015
    Applicant: BASF SE
    Inventors: Tilmann B. Walk, Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Hennicke Kamp, Jan C. Wiemer, Ralf Looser, Michael Manfred Herold, Alexandre Prokoudine
  • Publication number: 20150025339
    Abstract: Methods of classifying pleural fluid are disclosed. The methods typically include determining the level of indicator nanoparticles, such as lipids, particularly large lipids, in the pleural fluid of a subject. The level of lipids can be determined by nuclear magnetic resonance (NMR), such as proton NMR (1H-NMR) by measuring the NMR signal corresponding to methyl protons, methylene protons, methene protons, or combinations thereof. The level of large lipids in pleural fluid can be carried out in vitro on a sample of pleural fluid obtained from the subject or in vivo using magnetic resonance spectroscopy (MRS). The pleural fluid can be classified as exudate or transudate with a sensitivity, selectivity, or combination thereof of 85%, 90%, 95%, 99%, or higher, a selectivity of 85%, 90%, 95%, 99%. The method can be coupled with diagnosing and/or treating the subject with a disease, disorder, or condition.
    Type: Application
    Filed: July 18, 2014
    Publication date: January 22, 2015
    Inventor: Ching-Wan Lam
  • Publication number: 20150018244
    Abstract: The present invention pertains to the field of diagnostics for hyperthyroidism and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing hyperthyroidism. It also relates to a method for determining whether a compound is capable of inducing such hyperthyroidism in a subject and to a method of identifying a drug for treating hyperthyroidism. Furthermore, the present invention relates to a device and a kit for diagnosing hyperthyroidism.
    Type: Application
    Filed: March 7, 2013
    Publication date: January 15, 2015
    Applicant: BASF SE
    Inventors: Tilmann B. Walk, Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Hennicke Kamp, Jan C. Wiemer, Ralf Looser, Michael Manfred Herold, Alexandre Prokoudine
  • Patent number: 8932863
    Abstract: Methods for evaluating a fuel are provided. In one embodiment, a method of evaluating a fuel includes providing a testing specimen of the fuel. Also, the method includes analyzing the testing specimen and identifying a compound in the testing specimen. The method also provides for determining the fuel is biologically-sourced based on the identified trace compound.
    Type: Grant
    Filed: December 15, 2011
    Date of Patent: January 13, 2015
    Assignee: UOP LLC
    Inventors: Haiyan Wang, Michael J. McCall
  • Patent number: 8932865
    Abstract: A method that enables quantification of cholesterol in high-density lipoprotein 3 (HDL3) in a test sample without requiring a laborious operation is disclosed. The method for quantifying cholesterol in HDL3 having: Step 1 wherein a surfactant that reacts with lipoproteins other than high-density lipoprotein 3 is reacted with a test sample to transfer cholesterol to the outside of the reaction system; and Step 2 in which cholesterol remaining in the reaction system is quantified. The method enables specific quantification of HDL3 cholesterol in a test sample using an automatic analyzer without requirement of a laborious operation such as ultracentrifugation or pretreatment. Further, quantification of the HDL2 cholesterol level can also be carried out by subtracting the HDL3 cholesterol level from the total HDL cholesterol level obtained by a conventional method for quantifying the total HDL cholesterol in a test sample.
    Type: Grant
    Filed: July 22, 2011
    Date of Patent: January 13, 2015
    Assignee: Denka Seiken Co., Ltd.
    Inventors: Maiko Higuchi, Yasuki Itoh
  • Publication number: 20150011426
    Abstract: A novel non-enzymatic assay for lipid modulating drugs is being described for biological samples. For the first time non-enzymatic mechanism of statin drugs in modulating lipid aggregates and forming lipid particles is being shown. A simple flow cytometer based testing for detection of lipid particles, the effect of lipid modulating drug such as statin in vitro and testing for efficacy of the drug for an individual, dosage adjustment and drug discovery mechanism is being described. The lipid modulating drugs either individually or in combination can be discovered, optimized or efficacy tested using this assay. A novel statin induced lipid particle formation described can be used for diagnosis, personalized medicine and biomarker identification as well.
    Type: Application
    Filed: September 4, 2014
    Publication date: January 8, 2015
    Applicant: PLAXGEN INC
    Inventor: Shanmugavel Madasamy
  • Publication number: 20150011423
    Abstract: The present invention pertains to the field of diagnostics for kidney toxicity and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing kidney toxicity. It also relates to a method for determining whether a compound is capable of inducing such kidney toxicity in a subject and to a method of identifying a drug for treating kidney toxicity. Furthermore, the present invention relates to a device and a kit for diagnosing kidney toxicity.
    Type: Application
    Filed: September 14, 2012
    Publication date: January 8, 2015
    Applicant: BASF SE
    Inventors: Hennicke Kamp, Tilmann B. Walk, Bennard van Ravenzwaay, Werner Mellert, Eric Fabian, Volker Strauss, Jan C. Wiemer, Ralf Looser, Michael Manfred Herold, Alexandre Prokoudine
  • Publication number: 20140364452
    Abstract: The present invention provides a method of determining the susceptibility of a subject to treatment with a leukotriene modifier by determining the subject's cysteinyl leukotriene (CysLT) level and the subject's level of eosinophilic airway inflammation and identifying a subject with a high ratio of CysLT levels to eosinophilic airway inflammation as susceptible to treatment with the leukotriene modifier. Also discussed is a method of treatment of subjects who are susceptible to treatment that includes administering a leukotriene modifier to such a subject.
    Type: Application
    Filed: February 11, 2014
    Publication date: December 11, 2014
    Inventors: Nathan Rabinovitch, Erwin Gelfand
  • Patent number: 8906638
    Abstract: A method for quantifying remnant-like lipoprotein cholesterol in a sample simply and accurately without requiring separation operations, and a kit therefor are disclosed. A method for quantifying cholesterol in a remnant-like lipoprotein in a sample containing different lipoproteins including the remnant-like lipoprotein comprises a step (1) of erasing cholesterol in lipoproteins other than the remnant-like lipoprotein; and a step (2) of quantifying cholesterol in the remaining remnant-like lipoprotein. The step (1) is carried out under an action of a cholesterol esterase having a molecular weight of more than 40 kDa and not having a subunit having a molecular weight of not more than 40 kDa; and the step (2) is carried out under an action of a cholesterol esterase having a molecular weight of not more than 40 kDa or a cholesterol esterase having a subunit having a molecular weight of not more than 40 kDa.
    Type: Grant
    Filed: November 9, 2011
    Date of Patent: December 9, 2014
    Assignee: Denka Seiken Co., Ltd.
    Inventor: Yuhko Hirao
  • Publication number: 20140349885
    Abstract: A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological matrices. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.
    Type: Application
    Filed: December 6, 2012
    Publication date: November 27, 2014
    Inventors: Subhakar Dey, Subhasish Purkayastha