Immunological Separation Techniques Patents (Class 436/824)
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Patent number: 10317376Abstract: In one aspect, the disclosure provides methods of distinguishing a glycosaminoglycan from one or more other components in a sample by subjecting the sample to size-exclusion chromatography using a mobile phase having a pH of 6.8 or lower. A mobile phase having a pH of 6.8 or lower is found to improve the separation of glycosaminoglycans from proteins during size exclusion chromatography. In some embodiments, improved separation is due to the low pH of the mobile phase causing elution of less dispersed fractions of the protein and/or glycosaminoglycan. In some embodiments, the overlap between protein and/or glycosaminoglycan fractions is reduced.Type: GrantFiled: April 11, 2013Date of Patent: June 11, 2019Assignee: Bioverativ Therapeutics Inc.Inventors: Zoran Sosic, Andrew Blum, Bing Guan, Boris Boumajny
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Patent number: 9018361Abstract: Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.Type: GrantFiled: March 26, 2014Date of Patent: April 28, 2015Assignee: AbbVie Inc.Inventors: Robert K. Hickman, Qing Huang, Cheryl L. Weed, Scott T. Ennis, Barbara Perilli-Palmer, Min Wan
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Patent number: 8993254Abstract: An entity of a Th2 adjuvant activity in mother's milk has been revealed as coenzyme A by HPLC and mass spectrometry. The followings have been found out that: a risk of developing atopic dermatitis can be evaluated by targeting coenzyme A; and any one of a food and mother's milk with a reduced risk of developing atopic dermatitis can be prepared by removing or inactivating coenzyme A.Type: GrantFiled: October 26, 2010Date of Patent: March 31, 2015Assignee: Saitama Medical UniversityInventors: Sho Matsushita, Takehiro Higashi
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Patent number: 8911957Abstract: The invention features methods, devices, and kits for the isolation of analytes (e.g., a cell). A sample containing a desired analyte is introduced into a microfluidic device containing moieties that bind the desired analyte. A shear stress is applied that is great enough to prevent binding of undesired analytes and low enough to allow binding of the analyte of interest. Once bound, the desired analytes can be analyzed (e.g., counted). The invention also features methods for determining a shear stress for isolating a desired analyte.Type: GrantFiled: March 15, 2007Date of Patent: December 16, 2014Assignee: The General Hospital CorporationInventors: Daniel Irimia, Xuanhong Cheng, Mehmet Toner, Utkan Demirci, William Rodriguez
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Patent number: 8895709Abstract: Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.Type: GrantFiled: October 20, 2009Date of Patent: November 25, 2014Assignee: AbbVie Inc.Inventors: Robert K. Hickman, Qing Huang, Cheryl L. Weed, Scott T. Ennis, Barbara Perilli-Palmer, Min Wan
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Patent number: 8828738Abstract: The invention is directed to methods and devices for reducing interference from heterophile antibodies in an analyte immunoassay. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with non-human IgM or fragments thereof by dissolving into said sample a dry reagent to yield a non-human IgM concentration of at least about 20 ?g/mL or equivalent fragment concentration; and (b) performing an electrochemical immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG or fragments thereof in addition to the IgM of fragments thereof.Type: GrantFiled: December 6, 2011Date of Patent: September 9, 2014Assignee: Abbott Point of Care Inc.Inventors: John Lewis Emerson Campbell, John Emegbero Omakor
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Patent number: 8728828Abstract: The present invention relates to a separation matrix comprised of porous particles to which antibody-binding protein ligands have been immobilized, wherein the ligand density is in the range of 5.0-10 mg/ml; the gel phase distribution coefficient of the particles expressed as Kav for a dextran of size 110 kDa is above 0.65 and the median particle diameter is between 65-84 ?m. The carbohydrate material is preferably highly cross-linked agarose.Type: GrantFiled: December 22, 2005Date of Patent: May 20, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Hans Berg, Hans Johansson, Gunnar Malmquist, Per-Mikael Aberg
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Patent number: 8491904Abstract: Disclosed herein are methods for the isolation and purification of anti-IL-13 antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography (e.g., Protein A affinity chromatography), ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.Type: GrantFiled: October 20, 2010Date of Patent: July 23, 2013Assignee: Abbvie Inc.Inventor: Robert K. Hickman
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Patent number: 8343440Abstract: A cancer cell separating apparatus includes: a flow channel including an antibody fixation area having antibodies which bind specifically to cancer cells fixed thereon, wherein the cancer cells and non-cancer cells are separated using a difference in velocity of movement between the cancer cells and the non-cancer cells in cell slurry introduced into the flow channel.Type: GrantFiled: March 23, 2010Date of Patent: January 1, 2013Assignee: Seiko Epson CorporationInventor: Satomi Yoshioka
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Patent number: 8283169Abstract: A device and method in which cells of a heterogeneous population are separated through a column partially packed with antibody coated beads. The antibody coated beads may be placed in a gradient fashion such that each layer binds to and selects a subset of cells from the entire sample population. A lipoaspirate fluid, or other fluid containing cells, may be brought through the column by positive pressure on the specimen bag side and negative pressure at the base of the column with a vacuum line and waste trap. The column may then be inverted and irrigated internally with a wash buffer to remove unhomogenized macroscopic tissue material and unbound residual cells. The column may be closed at the ends and shaken and/or washed with an enzymatic buffer. Cells may be removed from the device with an elution buffer.Type: GrantFiled: March 8, 2008Date of Patent: October 9, 2012Inventor: Allan Yang Wu
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Patent number: 8247180Abstract: The invention provides methods and kits for detecting and/or measuring receptor homodimers on a cell surface membrane. In one aspect, the methods employ pairs of probes comprising binding compounds and a cleaving probe, such that at least one binding compound binds specifically to the same epitope of a membrane-bound analyte as the cleaving probe. The binding compound includes one or more molecular tags attached through a cleavable linkage, and the cleaving probe includes a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of the two probes to a homodimer of a cell surface molecules results in release of molecular tags from the binding compounds, providing a measure of formation of the homodimeric complex.Type: GrantFiled: July 18, 2008Date of Patent: August 21, 2012Assignee: Monogram Biosciences, Inc.Inventors: Sailaja Pidaparthi, Yining Shi, Rajiv Dua, Po-Ying Chan-Hui, Sharat Singh
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Patent number: 8198031Abstract: Methods are provided for detecting the formation of complexes of molecules, especially proteins, in a sample, such as a cell or tissue lysate. In one aspect, a cleaving probe specific for a first protein in a complex and one or more binding compounds specific for one or more second proteins in a complex are provided. Upon binding, the cleaving probe is induced to generate an active species, such as singlet oxygen, that cleaves molecular tags attached to the binding compounds only in the local region of the cleaving probe. The released molecular tags are separated from the assay mixture and from one another to provide a readout that is related to the number and types of proteins present in the complex.Type: GrantFiled: March 12, 2007Date of Patent: June 12, 2012Assignee: Monogram Biosciences, Inc.Inventors: Po-Ying Chan-Yui, Sharat Singh, Hossein Salimi-Moosavi, Yining Shi, Sailaja Pidaparthi, Rajiv Dua, Ali Mukherjee
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Patent number: 7939267Abstract: Methods, compositions and kits are provided for assessing angiogenesis through sensitive, direct detection of activation of endothelial cells at molecular levels. In general, activation of endothelial cells is detected by measuring the levels of cellular components and their protein complexes participating in a specific angiogenesis signaling pathway in endothelial cells. The methods can be used for assessing status of diseases associated with undesirable angiogenesis, such as the likelihood of developing the disease, presence or absence of the disease, prognosis of the disease and the likelihood of response or resistance to a particular anti-angiogenic therapy. The methods can also be used to guide the design of effective therapeutic regimens targeting a specific angiogenic signaling pathway, as well as in conjunction with therapeutic intervention of diseases or conditions associated with undesirable angiogenesis.Type: GrantFiled: November 3, 2005Date of Patent: May 10, 2011Assignee: Laboratory Corporation of America HoldingsInventors: Sean C. Moore, Sharat Singh, Hossein Salimi-Moosavi, Liching Cao, Jeff Sperinde
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Patent number: 7829347Abstract: A diagnostic test kit that provides an integrated system for accurately detecting a test analyte over a broad range of possible concentrations is provided. One feature of the integrated system is that it is capable of indicating whether an analyte is within the “hook effect” region. Based on this indication, a technique may be selected for correlating a measured signal intensity to an analyte concentration or range of concentrations. For example, when it is determined that the test sample falls outside the “hook effect” region, the analyte concentration may be determined using one portion of a dose response curve. On the other hand, when it is determined that the test sample falls within the “hook effect” concentration, the analyte concentration may be determined using another portion of the dose response curve. Alternatively, the sample may simply be diluted for re-performing the assay.Type: GrantFiled: August 31, 2005Date of Patent: November 9, 2010Assignee: Kimberly-Clark Worldwide, Inc.Inventor: Xuedong Song
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Patent number: 7772007Abstract: An assay device and method for measuring the concentration of LDL-associated cholesterol in a blood-fluid sample are described. The method employs selective precipitation of VLDL and chylomicrons and immunoseparation of HDL from a blood fluid sample. The assay device allows the assay to be performed entirely in a flow strip format.Type: GrantFiled: March 31, 2005Date of Patent: August 10, 2010Assignee: Cholestech CorporationInventors: Jeffrey E. Shindelman, Thomas E. Worthy, Ronald M. Jones, George E. Withers, III
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Patent number: 7709212Abstract: The present invention provides a method for detecting the presence or amount of an analyte in a sample, said method comprising: a porous surface to which particles having attached thereto a binding substance, analyte and binding substance coated label particles are added. If said analyte is present in the sample an immuno- or chemical reaction occurs in the liquid phase. The separation of bound complex from unbound material is achieved by using said surface where the separation occurs mainly two dimensionally on the surface of the porous surface (e.g. grid). Interestingly, the disclosed surface enables a separation where said complexes are distributed two dimensionally on the porous surface (e.g. grid), whereas unbound materials are distributed three dimensionally. Accordingly, said surface enables both a two and a three dimensional separation.Type: GrantFiled: November 23, 2005Date of Patent: May 4, 2010Assignee: Orion Diagnostica OyInventors: Juhani Luotola, Hannu Nikula, Mira Murtovuori
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Patent number: 7670786Abstract: A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a self-calibrated magnetic binding assay format (e.g., sandwich, competitive, etc.) that includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating a calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional (e.g., directly or inversely) to the intensity of the detection signal calibrated by the intensity of the calibration signal. It has been discovered that the fluidics-based device of the present invention provides an accurate, inexpensive, and readily controllable method of determining the presence of an analyte in a test sample.Type: GrantFiled: October 22, 2007Date of Patent: March 2, 2010Assignee: Kimberly-Clark Worldwide, Inc.Inventors: Xuedong Song, Rosann Marie Matthews Kaylor
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Patent number: 7645614Abstract: The present invention provides a method of analyzing the specific interaction between a molecule to be analyzed and a molecule that specifically interacts with the former molecule on a solid phase using a molecule-immobilized solid phase support mixture prepared by binding the subject molecule to the solid phase support without specifying the binding position on the molecule side, particularly a method wherein the immobilization is conducted via a spacer introduced to the molecule without specifying the binding position on the molecule side, which method makes it possible to identify and select only a molecule that exhibits a specific interaction with a molecule to be analyzed, without an investigation of structure-activity correlation, which has conventionally been essential, and hence enables an analysis of the interaction between these molecules.Type: GrantFiled: October 31, 2003Date of Patent: January 12, 2010Assignee: Reverse Proteomics Research Institute Co., Ltd.Inventors: Akito Tanaka, Tomohiro Terada, Akira Yamazaki, Tsuruki Tamura, Hidenori Nakajima
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Patent number: 7605003Abstract: The present invention relates to a method of qualifying ovarian cancer status in a subject comprising: (a) measuring at least one biomarker in a sample from the subject and (b) correlating the measurement with ovarian cancer status. The invention further relates to kits for qualifying ovarian cancer status in a subject.Type: GrantFiled: August 5, 2003Date of Patent: October 20, 2009Assignees: The Johns Hopkins University, Vermillion, Inc.Inventors: Daniel W. Chan, Zhen Zhang, Eric Fung, Xiao-Ying Meng
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Patent number: 7601335Abstract: The present application describes a method for pretreating a biological sample from an autoimmune disease subject in order to avoid interference, especially where the sample is to be subjected to a cell-based biological activity assay, such as a neutralizing antibody assay.Type: GrantFiled: May 19, 2006Date of Patent: October 13, 2009Assignee: Genentech, Inc.Inventors: Krista McCutcheon, An Song
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Patent number: 7524641Abstract: Rare cells are separated from a sample fluid by a positive selection or negative selection antibody by centrifuging in a tube containing a harvesting float. The harvesting float has an axial passage and a density to settle in the sample fluid and expand the layer of the target component. The positive selection antibody is preferably coupled to a particulate carrier, such as a microbead, to attach to the target component. The negative selection antibody forms a complex or conjugate with a contaminating component. In the positive separation, the particulate carrier is recovered in the axial passage of the float.Type: GrantFiled: March 12, 2007Date of Patent: April 28, 2009Assignee: Becton, Dickinson and CompanyInventors: Stewart Russell Jurgensen, Sheila Ann Lloyd
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Patent number: 7465587Abstract: This invention relates to assays for an analyte in a liquid sample such as a body fluid. More particularly, the invention relates to a method and apparatus for the detection of a ligand in a body fluid such as urine or blood, which includes an immobilization zone for interfering agents in a sample.Type: GrantFiled: December 3, 2004Date of Patent: December 16, 2008Assignee: Genzyme CorporationInventor: Michael Reece Imrich
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Patent number: 7455985Abstract: The present invention provides methods and compositions for detecting bacteria of the Chlamydiaceae family in a biological sample. Methods include, for example, contacting a biological sample with an antibody that specifically binds to a chlamydial antigen displayed on the surface of a chlamydia-infected blood cell; and analyzing the sample using fluorescence microscopy or flow cytometry to detect bound antibody.Type: GrantFiled: April 15, 2005Date of Patent: November 25, 2008Assignee: University of MassachusettsInventors: Elizabeth S. Stuart, Lloyd H. Semprevivo
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Patent number: 7402397Abstract: Methods are provided for detecting the formation of complexes of molecules, especially proteins, in a sample, such as a cell or tissue lysate. In one aspect, a cleaving probe specific for a first protein in a complex and one or more binding compounds specific for one or more second proteins in a complex are provided. Upon binding, the cleaving probe is induced to generate an active species, such as singlet oxygen, that cleaves molecular tags attached to the binding compounds only in the local region of the cleaving probe. The released molecular tags are separated from the assay mixture and from one another to provide a readout that is related to the number and types of proteins present in the complex.Type: GrantFiled: August 10, 2004Date of Patent: July 22, 2008Assignee: Monogram BioSciences, Inc.Inventors: Po-Ying Chan-Hui, Sharat Singh, Hossein Salimi-Moosavi, Hasan Tahir, Gerald J. Wallweber, Hrair Kirakosssian, Tracy J. Matray
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Patent number: 7402398Abstract: The invention provides methods and kits for detecting and/or measuring receptor homodimers on a cell surface membrane. In one aspect, the methods employ pairs of probes comprising binding compounds and a cleaving probe, such that at least one binding compound binds specifically to the same epitope of a membrane-bound analyte as the cleaving probe. The binding compound includes one or more molecular tags attached through a cleavable linkage, and the cleaving probe includes a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of the two probes to a homodimer of a cell surface molecules results in release of molecular tags from the binding compounds, providing a measure of formation of the homodimeric complex.Type: GrantFiled: September 22, 2004Date of Patent: July 22, 2008Assignee: Monogram BioSciences, Inc.Inventors: Sailaja Pidaparthi, Yining Shi, Rajiv Dua, Po-Ying Chan-Hui, Sharat Singh
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Patent number: 7371584Abstract: Novel magnetic assay methods and systems. According to a preferred embodiment, a chromatographic medium is provided that is designed to be contacted with a test solution having activated magnetic particles such that the solution flows bilaterally thereacross. A magnetic field, generated by a magnet or electromagnet, is selectively applied to the medium which causes the charged particles to become substantially bound at a site on the medium specified by the position of the magnet. The assay is multi-configurable and can be modified to isolate target cells and grow cell cultures.Type: GrantFiled: December 8, 2006Date of Patent: May 13, 2008Assignee: Wavesence, Inc.Inventor: Christopher Feistel
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Patent number: 7368277Abstract: The invention relates to a technology by which antibodies directed to sources of infection in body fluids can be assayed with high accuracy, expediency and specificity. More particularly, the invention provides an antibody immunoassay method in which the antigen-antibody reaction between a target antibody in a sample and an assay antigen is conducted in the presence of an E. coli component and an antibody assay method which comprises using a reagent having a specific affinity for the Fc region of an antibody IgG as the antibody assay reagent.Type: GrantFiled: March 13, 2003Date of Patent: May 6, 2008Assignee: Otsuka Pharmaceutical Factory, Inc.Inventors: Tetsuya Tachikawa, Atsunari Noda, Kiyonori Katsuragi
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Patent number: 7364921Abstract: A method for separating biological materials and other substances is disclosed, wherein a mixture containing desired and undesired components are exposed to magnetic particles having ligands capable of binding to the desired and/or the undesired components to form a magnetic mixture, placing the magnetic mixture onto a substrate material; exposing the substrate coated with the magnetic mixture to a magnetic field of sufficient strength to cause the magnetic components to migrate across the substrate; and repeatedly increasing and decreasing the magnetic field in a pulsing manner with a frequency sufficient to cause the desired magnetic components to separate spatially from the undesired magnetic components. A device for separating biological materials capable of being operated to increase and decrease magnetic field in a pulse fashion is also disclosed.Type: GrantFiled: January 6, 2000Date of Patent: April 29, 2008Assignee: University of Medicine and Dentistry of New JerseyInventors: Leonard Sciorra, Joseph Zimnoch
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Patent number: 7358096Abstract: The immobilization of proteins at solid surfaces facilitated by incorporation of a polypeptide segment which is capable of adopting a folded structure is described. Materials comprising proteins so immobilized and uses thereof are also described.Type: GrantFiled: November 22, 2000Date of Patent: April 15, 2008Assignee: Conopco, Inc.Inventors: Steven Daryl Grant, Steven Howell, Stephen Wilson
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Patent number: 7316932Abstract: The present invention is related to a method for separating a first population of cells from a second population of cells in a sample by discontinuous density gradient separation using dense particles to target the first population of cells and a density separation medium (DSM) that is at least about 0.001 g/cm3 higher than the density of the second population of cells.Type: GrantFiled: October 1, 2002Date of Patent: January 8, 2008Assignee: Stemcell Technologies Inc.Inventor: Steven M. Woodside
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Patent number: 7285424Abstract: A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a self-calibrated magnetic binding assay format (e.g., sandwich, competitive, etc.) that includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating a calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional (e.g., directly or inversely) to the intensity of the detection signal calibrated by the intensity of the calibration signal. It has been discovered that the fluidics-based device of the present invention provides an accurate, inexpensive, and readily controllable method of determining the presence of an analyte in a test sample.Type: GrantFiled: August 27, 2002Date of Patent: October 23, 2007Assignee: Kimberly-Clark Worldwide, Inc.Inventors: Xuedong Song, Rosann Kaylor
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Patent number: 7252961Abstract: The invention relates to a method for the detection of an analyte in a competitive immunoassay in the presence of an analyte derivative and first and second receptor molecules, and to a kit for carrying out this detection method.Type: GrantFiled: July 17, 1997Date of Patent: August 7, 2007Assignee: Dade Behring Marburg GmbHInventors: Stephan Neuenhofer, Heinz-Jürgen Skrzipczyk, Peter Molz, Reinhard Käsmarker
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Patent number: 7223546Abstract: There is provided a method and kit for measuring the amount of an objective constituent contained in a specific lipoprotein in a biological sample such as serum and plasma, specifically for measuring the amount of cholesterol contained in high density lipoprotein, which can be applicable to clinical tests.Type: GrantFiled: March 26, 2003Date of Patent: May 29, 2007Assignee: Wako Pure Chemical Industries, Ltd.Inventors: Yutaka Miki, Toshiro Hanada, Kiyoko Tanaka
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Patent number: 7205157Abstract: Rare cells are separated from a sample fluid by a positive selection or negative selection antibody by centrifuging in a tube containing a harvesting float. The harvesting float has an axial passage and a density to settle in the sample fluid and expand the layer of the target component. The antibody is preferably coupled to a particulate carrier, such as a microbead, to attach either the target component or a contaminating component to the particulate carrier. In the positive separation, the particulate carrier is recovered in the axial passage of the float.Type: GrantFiled: January 8, 2001Date of Patent: April 17, 2007Assignee: Becton, Dickinson and CompanyInventors: Stewart Russell Jurgensen, Sheila Ann Lloyd
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Patent number: 7183119Abstract: The present invention relates to a method of detecting biological analytes comprising suspending a target analyte in a suspending solution containing polymeric particles marked with a probe, wherein the probe has an affinity for said target analyte; adding recognition unit-peroxidase conjugate marker to the suspending solution; forming a complex of the target analyte, the polymeric particles marked with a probe, and the recognition unit-peroxidase conjugate marker; contacting a gelatin surface with the suspending solution; adding developer to the suspending solution in contact with the gelatin surface in the presence of phenol to attach the complex to the gelatin surface; washing the gelatin surface; and detecting the complex attached to the gelatin surface.Type: GrantFiled: November 15, 2004Date of Patent: February 27, 2007Assignee: Eastman Kodak CompanyInventors: Tiecheng A. Qiao, Brian J. Kelley
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Patent number: 7166423Abstract: Cells can be labeled with products which they secrete and release in an efficient manner by coupling the cells at their surface to a specific binding partner for the product and allowing the product to be captured by the specific binding partner as it is secreted and released. The product-labeled cells can then be further coupled to suitable labels, if desired, and separated according to the presence, absence, or amount of product.Type: GrantFiled: October 21, 1993Date of Patent: January 23, 2007Assignee: Miltenyi Biotec GmbHInventors: Stefan Miltenyi, Andreas Radbruch, Rudi Manz
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Patent number: 7160735Abstract: Compositions and methods are disclosed for detecting multiple target analytes in a sample using microparticles having molecular tags attached by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such microparticles under conditions that permit stable complexes to form between binding moieties on the surfaces of the microparticles and the analytes. In one aspect of the invention, the a second binding composition is added so that complexes form among the microparticle-bound binding moieties, the analytes, and second binding moieties of the second binding composition. Such second binding moieties have cleavage-inducing moieties attached that upon activation cause the cleavage of the cleavable linkages and the release of molecular tags. Released molecular tags are separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.Type: GrantFiled: November 8, 2002Date of Patent: January 9, 2007Assignee: Monogram Biosciences, Inc.Inventors: Peter Dehlinger, Sharat Singh, Hrair Kirakossian
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Patent number: 7122382Abstract: A method for detecting proteins under mutual interaction which comprises mixing a protein having a label for detection synthesized by a cell-free protein synthesis method with another protein having a modification for separation, or synthesizing a protein having a label for detection and another protein having a modification for separation in a single system by the cell-free protein synthesis method; separating a pair of proteins formed by the interaction between these proteins with the use of the modification for separation as described above; and distinguishing these proteins with the use of the label for detection as described above. A screening method using this method.Type: GrantFiled: April 28, 2000Date of Patent: October 17, 2006Assignee: RikenInventor: Yoshihide Hayashizaki
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Patent number: 7108985Abstract: Methods and reagents for obtaining simplified mixtures of peptides from a sample containing a number of peptides are disclosed. The simplified sample can be easier to analyze than the original peptide sample yet it is representative of all or nearly all of the proteins present in the mixed protein sample from which the original and more complex peptide sample was derived. The methods entail the use of tagging moieties that include an amino-acid-specific reactive group (R). The tagging moieties “tag” peptides or proteins at specific amino acids (e.g., by reacting with an amino acid to form a covalent bond), ultimately allowing the isolation of peptides that contain those specific amino acids. Other methods entail the used of a reactive moiety (RP) that comprises a reagent that selectively interacts with selected proteins, either covalently or noncovalently. For example, RP can be a natural ligand for a receptor that is to be tagged or a protein that interacts with a second protein that is to be tagged.Type: GrantFiled: April 3, 2002Date of Patent: September 19, 2006Assignee: Thermo Finnigan, LLCInventors: Christopher Becker, James R. LaDine, Thomas A. Shaler, Chia-Hui Paul Shieh
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Patent number: 7074622Abstract: The present invention relates to a method of particle separation comprising preparing a suspension of particles containing at least one recognizable target particle in a suspending solution, labeling the target particle with a conjugate marker, wherein the conjugate marker comprises at least one recognition unit for the recognizable target particle and at least one peroxidase enzyme, contacting a gelatin surface with the suspending solution, adding developer to the suspending solution in contact with the gelatin surface and in the presence of phenol to attach the target particle to the gelatin surface, and washing the gelatin surface to remove unattached particles. The method may also include detecting the presence of the target particle on the gelatin surface as well as detaching and recovering the attached particles after removal of the non-target particles.Type: GrantFiled: November 15, 2004Date of Patent: July 11, 2006Assignee: Eastman Kodak CompanyInventors: Tiecheng A. Qiao, Yun C. Chang, Eric R. Schmittou, Thomas I. Penner
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Patent number: 7064191Abstract: The present invention relates to a process for purifying an antibody having a desired property, which comprises using a substance having an affinity to a carbohydrate binding to the antibody; a medicament comprising, as an active ingredient, the antibody purified by the process; and a method for diagnosing or preventing various diseases, which comprises using a substance having an affinity to a carbohydrate binding to an antibody.Type: GrantFiled: October 4, 2001Date of Patent: June 20, 2006Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Toyohide Shinkawa, Kazuhisa Uchida, Motoo Yamasaki, Emi Hosaka, Kenya Shitara
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Patent number: 7056657Abstract: Separation apparatus and method for separating magnetic and/or magnetically-labeled particles from a test medium. Test medium within a reaction chamber is caused to flow past a collecting surface, and a high-gradient magnetic field is applied to the surface to capture magnetically responsive particles in the test medium. The particles are deflected toward the collection surface by baffles, a spinner, or a sprayer, or are funneled past the surface by a plunger operable to be displaced into close proximity to the surface to provide a narrow flow path for the particle-laden test medium. The particles normally suspended in the medium are separated out of suspension by adhesion to the collection surface. The particles may be resuspended by removal of the surface from the high-gradient field, or removal of the high-gradient field from the surface. The collection surface is a thin-walled non-magnetic material having a plurality of magnetic pole faces positioned therearound.Type: GrantFiled: February 6, 2002Date of Patent: June 6, 2006Assignee: ImmuniVest CorporationInventors: Leon W.M.M. Terstappen, Gerald V. Doyle, Paul A. Liberti, Gerald J. Dolan
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Patent number: 7045363Abstract: A nucleic acid-bound polypeptide produced by binding a nucleic acid to a polypeptide, a method of producing the nucleic acid-bound polypeptide, and applications of the nucleic acid-bound polypeptide, including immunoassays for an antigen or antibody, such as an agglutination immunoassay are provided.Type: GrantFiled: May 7, 1999Date of Patent: May 16, 2006Assignee: Fujirebio Inc.Inventors: Fuminori Takemura, Eiichi Ueno, Satoru Itoh
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Patent number: 7022491Abstract: The invention relates to a method for monitoring interactions to a target biomolecule comprising the steps of: providing a biomolecule of interest having specificity for the target biomolecule; binding the biomolecule of interest to at least one type of linker molecule comprising a unique mass marker part; introducing the biomolecule of interest to a cell; binding the linker to the target biomolecule; cleaving the linker molecule, thereby leaving the photoactivatable part and the mass marker part bound to the target; analysing the target biomolecule, thereby detecting the unique mass marker part. The detection can be carried out by MS in a parent ion scanning mode, thereby allowing study of the interaction between the biomolecule of interest and the target biomolecule.Type: GrantFiled: December 16, 2002Date of Patent: April 4, 2006Inventor: Peter James
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Patent number: 7001775Abstract: A method and kit for screening a sample of body fluid for at least one autoantibody to at least one antigen. A source of at least one antigen to the autoantibody is provided. A substrate having immobilized thereto at least one antibody to the antigen is also provided. The antigen source is contacted with the sample of body fluid, so as to obtain a mixture wherein the antigen is allowed to substantially bind with the autoantibody, when the latter is present in the sample of body fluid. The mixture is allowed to flow relative to the substrate so as to allow the mixture to contact the antibody immobilized to the substrate. Labeling means are provided to permit monitoring of binding of the autoanitbody and the antigen present in the mixture, so as to provide an indication of the presence of the autoanitbody in the sample of body fluid.Type: GrantFiled: October 27, 1999Date of Patent: February 21, 2006Assignee: RSR LimitedInventors: Peter John Burne, Bernard Rees Smith
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Patent number: 6991912Abstract: Novel magnetic assay methods and systems. According to a preferred embodiment, a chromatographic medium, which preferably comprises a test strip, is provided that is designed to be contacted with a test solution having activated magnetic particles such that the solution flows bilaterally thereacross. A magnetic field, generated by a magnet or electromagnet, is selectively applied to the medium which causes the charged particles to become substantially bound at a site on the medium specified by the position of the magnet, to thus form a captured line or zone. In one preferred embodiment, the magnetic field is applied at the site on the medium at which the test solution is contacted. The degree of magnetic force applied to the membrane may be selectively adjusted to vary the width or surface area of the capture line or zone.Type: GrantFiled: February 5, 2002Date of Patent: January 31, 2006Assignee: Wavesesense, LLCInventor: Christopher Feistel
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Patent number: 6972327Abstract: The invention provides improved methods of regenerating and using affinity chromatography material, in particular Protein A affinity chromatography resins.Type: GrantFiled: May 8, 2002Date of Patent: December 6, 2005Assignee: Immunex CorporationInventors: Hassan Madani, Robert D. Hershberg
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Patent number: 6939681Abstract: The invention relates to a method of evaluating the immunological status of a subject comprising the steps of 1) determining the content of an antibody in a liquid sample from the subject using an immunoassay, wherein the reaction between the antibody of the sample and a ligand in the form of an antigen, an antibody or a hapten, the ligand being directed to the Fab region of the sample antibody, is carried out in the presence of other constituents of the sample to obtain a measurement 1, 2) determining the content of an antibody in the liquid sample using an immunoassay, wherein the reaction between the antibody of the sample and a ligand in the form of an antigen, an antibody or a hapten, the ligand being directed to the Fab region of the sample antibody, is carried out in the absence of other constituents of the sample to obtain a measurement 2, and 3) interrelating measurements 1 and 2 to express the interference and using the interference as a parameter for evaluating the immunological status of the subjeType: GrantFiled: February 28, 2002Date of Patent: September 6, 2005Assignee: Alk-Abello A/SInventors: Hans-Henrik Ipsen, Niels Johansen, Rikke Morkeberg, Soren Bogestrand, Tine Charlotte Beck
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Patent number: 6933109Abstract: Methods and reagents for rapid purification and/or identification of particles in a liquid sample are described. The technique uses centrifugation to concentrate particles against a slanted surface having an agent specifically binding to the particles. This method is applicable for the rapid identification of viruses and other difficult or impossible to culture microorganisms without replication or amplification of the microorganism.Type: GrantFiled: December 22, 2000Date of Patent: August 23, 2005Assignee: Large Scale Proteomics CorporationInventor: Norman G. Anderson
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Patent number: RE43979Abstract: The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies directed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotincomplexes or certain enzymes allowing visualization, with dramatically increase the specificity of the method. The method can further be used for isolation of the target-cells by magnetic field application and kit for performing the method according to the invention is described.Type: GrantFiled: February 5, 2003Date of Patent: February 5, 2013Assignee: Abbott LaboratoriesInventors: Oystein Fodstad, Gunnar Kvalheim