Abstract: An inoculation system for a bee hive having a hive opening includes (a) a body mountable to the hive intermediate the hive opening and an outside environment external the hive, (b) a bee pathway in the body for providing passage between the hive opening and the outside environment when the body is mounted to the hive, (c) a removable cartridge installed in the body and having a cartridge chamber storing an inoculum, and (d) an automatic inoculum dispenser in the body. The dispenser is operable to controllably dispense the inoculum from the cartridge chamber into the bee pathway to dust bees passing through the bee pathway with the inoculum.