Using A Dynamic Combinatorial Chemistry Technique Patents (Class 506/25)
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Patent number: 11466268Abstract: A structured substrate includes a substrate body having an active side. The substrate body includes reaction cavities that open along the active side and interstitial regions that separate the reaction cavities. The structured substrate includes an ensemble amplifier positioned within each of the reaction cavities. The ensemble amplifier includes a plurality of nanostructures configured to at least one of amplify electromagnetic energy that propagates into the corresponding reaction cavity or amplify electromagnetic energy that is generated within the corresponding reaction cavity.Type: GrantFiled: December 21, 2020Date of Patent: October 11, 2022Assignee: ILLUMINA, INC.Inventors: M. Shane Bowen, Dajun Yuan
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Patent number: 9895435Abstract: Compositions of recombinant HSV-2 proteins and an agonist of the innate immune system, such as an adjuvant, are provided as a vaccine. Proteins include an envelope glycoprotein and a structural protein other than an envelope glycoprotein, e.g., a capsid or tegument protein. The vaccine is for use in either HSV-2 seropositive or seronegative subjects.Type: GrantFiled: May 16, 2013Date of Patent: February 20, 2018Assignee: IMMUNE DESIGN CORP.Inventors: Thomas W. Dubensky, Jr., Nancy A. Hosken, Scott H. Robbins, Margaret D. Moore
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Patent number: 9555099Abstract: Compositions of recombinant HSV-2 proteins and an agonist of the innate immune system, such as an adjuvant, are provided as a vaccine. Proteins include an envelope glycoprotein and a structural protein other than an envelope glycoprotein, e.g., a capsid or tegument protein. The vaccine is for use in either HSV-2 seropositive or seronegative subjects.Type: GrantFiled: May 16, 2013Date of Patent: January 31, 2017Assignee: IMMUNE DESIGN CORP.Inventors: Thomas W. Dubensky, Jr., Nancy A. Hosken, Scott H. Robbins, Margaret D. Moore
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Patent number: 8853185Abstract: A monomer useful in prepa?ng therapeutic compounds includes a diversity element which potentially binds to a target molecule with a dissociation constant of less than 300 11 M and a linker element connected to the diversity element The linker element has a molecular weight less than 500 daltons, is connected, directly or indirectly through a connector, to said diversity element, and is capable of forming a reversible covalent bond or noncovalent interaction with a binding partner of the linker element The monomers can be covalently or non-covalently linked together to form a therapeutic multimer or a precursor thereof.Type: GrantFiled: April 9, 2009Date of Patent: October 7, 2014Assignees: Cornell University, Purdue Research FoundationInventors: Francis Barany, Maneesh Pingle, Donald Bergstrom, Sarah Filippa Giardina
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Patent number: 8815782Abstract: Provided herein is method comprising: contacting an initial RNA sample containing a population of different RNA molecules with a divalent cation and a set of DNAzymes that are designed to cleave multiple target RNAs in the initial sample, thereby producing a product RNA sample that comprises: a) uncleaved RNA molecules and b) cleaved RNA fragments that contain a 2?,3?-cyclic-phosphate and a 5? hydroxyl as the result of DNAzyme cleavage.Type: GrantFiled: November 1, 2012Date of Patent: August 26, 2014Assignee: Agilent Technologies, Inc.Inventors: Gusti Zeiner, Robert A. Ach
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Publication number: 20140045707Abstract: Improved compositions, methods, apparatus, and kits for high-throughput nucleic acid amplification, detection and sequencing are disclosed. A nucleic acid cluster having an identifiable center is produced by generating on a solid support an immobilized nucleic acid complement from a template, one of which comprises a detectable label; and amplifying the complement and the template to obtain a nucleic acid cluster on the support, the cluster having a substantially central location marked by the detectable label and a surrounding region comprising immobilized copies. Also disclosed are nucleotide sequence determination in nucleic acid clusters so produced, center position annotation in the clusters, assignment of sequence information to overlapping clusters, and related compositions and methods.Type: ApplicationFiled: September 6, 2013Publication date: February 13, 2014Applicant: Illumina Cambridge LimitedInventors: Alan D. Kersey, Jonathan Mark Boutell
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Patent number: 8618024Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.Type: GrantFiled: August 31, 2006Date of Patent: December 31, 2013Assignee: Gendaq LimitedInventors: Yen Choo, Aaron Klug, Mark Isalan
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Publication number: 20130310417Abstract: The present invention provides small molecules for endothelial cell activation and compositions thereof and methods of making and using the same.Type: ApplicationFiled: February 23, 2012Publication date: November 21, 2013Applicant: The Regents of the University fo CaliforniaInventors: Daniel Cruz, Ohyun Kwon
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Patent number: 8575070Abstract: The present invention provides the means for producing libraries of peptide structures for drug screening applications that are capable of folding or assuming their native conformations independently of artificial scaffolds or flanking sequences in the proteins from which they are derived. The libraries can be highly diverse such that they are representative of the repertoire of protein structures existing in nature. The libraries can also be non-redundant or normalized such that the bias towards specific structures existing in source data sets and/or in nature is/are removed. In a particularly preferred embodiment, the present invention provides 30,000 independent fold structures produced by this method. The present invention also provides computer-readable media and systems comprising structural data in relation to the peptide libraries, and methods for displaying and screening the libraries.Type: GrantFiled: February 7, 2007Date of Patent: November 5, 2013Assignee: Phylogica LimitedInventors: Paul Michael Watt, Roland Dunbrack
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Publication number: 20120190586Abstract: A method of making a synthetic organic receptor that specifically binds to a modified amino acid is carried out by: reacting a plurality of monomers in a dynamic combinatorial library in the presence of a protein or peptide to form a reaction product, the protein or peptide comprising a modified nucleic acid, with the reacting step carried out under conditions in which at least one oligomer that specifically binds to the modified amino acid is selectively amplified in the reaction product; and then isolating or identifying from the reaction product at least one oligomer that specifically binds to the modified amino acid.Type: ApplicationFiled: October 13, 2010Publication date: July 26, 2012Inventors: Marcey L. Waters, Lindsey Ingerman James
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Publication number: 20120015849Abstract: The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.Type: ApplicationFiled: June 7, 2011Publication date: January 19, 2012Applicant: DANISCO US INC.Inventors: Philippe Soucaille, Marguerite A. Cervin, Fernando Valle
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Publication number: 20110281773Abstract: An integrated processing tool is described comprising a full-wafer processing module and a combinatorial processing module. Chemicals for use in the combinatorial processing module are fed from a delivery system including a set of first manifolds. An output of each first manifold is coupled to at least one mixing vessel. An output of each mixing vessel feeds more than one of a set of second manifolds. An output of each set of second manifolds feeds one of multiple site-isolated reactors of the combinatorial processing module.Type: ApplicationFiled: July 25, 2011Publication date: November 17, 2011Applicant: INTERMOLECULAR, INC.Inventors: Kurt H. Weiner, Tony P. Chiang, Aaron Francis, John Schmidt
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Publication number: 20110224086Abstract: The present invention relates to methods and algorithms for identifying, synthesizing and co-assembling combinatorial libraries of polynucleotide variants.Type: ApplicationFiled: March 9, 2010Publication date: September 15, 2011Inventors: Jose Pardinas, Shanrong Zhao
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Patent number: 7972994Abstract: The present invention provides methods of synthesizing libraries of molecules comprising a functional moiety which is operatively linked to an encoding oligonucleotide, wherein the encoding oligonucleotide comprises a capping sequence containing degenerate nucleotides.Type: GrantFiled: June 9, 2006Date of Patent: July 5, 2011Assignee: GlaxoSmithKline LLCInventors: Barry Morgan, Stephen Hale, Christopher C. Arico-Muendel, Matthew Clark, Richard Wagner, David I. Israel, Malcolm L. Gefter, Dennis Benjamin, Nils Jakob Vest Hansen, Malcolm J. Kavarana, Steffen Phillip Creaser, George J. Franklin, Paulo A. Centrella, Raksha A. Acharya
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Publication number: 20100273660Abstract: In some aspects, the invention provides a genetically tractable in situ non-human animal model for hepatocellular carcinoma. The model is useful, inter alia, in understanding the molecular mechanisms of liver cancer, in understanding the genetic alterations that lead to chemoresistance or poor prognosis, and in identifying and evaluating new therapies against hepatocellular carcinomas. The liver cancer model of this invention is made by altering hepatocytes to increase oncogene expression, to reduce tumor suppressor gene expression or both and by transplanting the resulting hepatocytes into a recipient non-human animal. The present invention also provides methods for identifying and validating tumor suppressor genes by screening pools of shRNAs that target genomic regions deleted in human cancers, such as human hepatocellular carcinomas.Type: ApplicationFiled: November 12, 2009Publication date: October 28, 2010Applicant: Cold Spring Harbor LaboratoryInventors: Lars Zender, Wen Xue, Scott Powers, Scott W. Lowe
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Patent number: 7767627Abstract: Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials or, alternatively, allowing the components to interact to form at least two different materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, nonbiological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc.Type: GrantFiled: April 22, 1997Date of Patent: August 3, 2010Assignees: Symyx Solutions, Inc., The Regents of the University of CaliforniaInventors: Isy Goldwasser, Debra A. Ross, Peter G. Schultz, Xiao-Dong Xiang, Gabriel BriceƱo, Xian-Dong Sun, Kai-An Wang
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Publication number: 20100184702Abstract: This invention provides novel polydentate selective high affinity ligands (SHALs) that can be used in a variety of applications in a manner analogous to the use of antibodies. SHALs typically comprise a multiplicity of ligands that each bind different region son the target molecule. The ligands are joined directly or through a linker thereby forming a polydentate moiety that typically binds the target molecule with high selectivity and avidity.Type: ApplicationFiled: December 18, 2009Publication date: July 22, 2010Inventors: Sally DeNardo, Gerald DeNardo, Rodney Balhorn
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Publication number: 20090285825Abstract: This invention is in the field of snake venom and the invention provides a novel snake toxin protein and nucleic acids encoding the same. Also, provided are various uses and compositions based on the discovery of the novel snake toxin.Type: ApplicationFiled: December 22, 2005Publication date: November 19, 2009Applicant: National University of SingaporeInventors: Manjunatha Ramachandra Kini, Yuh Fen Fung, Prakash Pallathadka Kumar, Peter Tsun Hon Wong, Chung Pin Teo
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Publication number: 20090018028Abstract: The present invention provides self-assembling, finite nucleic acid tiling arrays, and methods for their synthesis and use, which overcome a major hurdle in self-assembled DNA nanostructures, and therefore have numerous potential applications for nanofabrication of complex structures and useful devices, as further disclosed herein.Type: ApplicationFiled: February 15, 2006Publication date: January 15, 2009Inventors: Stuart Lindsay, Hao Yan, John Chaput, Yan Liu, Peiming Zhang
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Publication number: 20090011956Abstract: The present invention relates generally to programming of biomolecular self-assembly pathways and related methods and constructs. A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways for a wide variety of dynamic functions, reaction graphs for specifying pathways, and methods of using the hairpin motif are provided.Type: ApplicationFiled: May 16, 2008Publication date: January 8, 2009Inventors: Peng Yin, Niles A. Pierce
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Publication number: 20080070801Abstract: The present invention relates to methods and apparatus for synthesizing combinatorial materials libraries using pyrolysis techniques. In certain embodiments, the methods involve varying the precursors and/or reactant gases in an operating pyrolysis unit to continuously vary the resulting nanoparticle composition and collecting different nanoparticles at different locations on a substrate using a spatially addressable particle collector.Type: ApplicationFiled: October 13, 2005Publication date: March 20, 2008Inventors: Xiao-Dong Xiang, Young K. Yoo, Yi-Qun Li, Daesig Kim, Sohrab Erfanian