Using A Dynamic Combinatorial Chemistry Technique Patents (Class 506/25)
  • Patent number: 9555099
    Abstract: Compositions of recombinant HSV-2 proteins and an agonist of the innate immune system, such as an adjuvant, are provided as a vaccine. Proteins include an envelope glycoprotein and a structural protein other than an envelope glycoprotein, e.g., a capsid or tegument protein. The vaccine is for use in either HSV-2 seropositive or seronegative subjects.
    Type: Grant
    Filed: May 16, 2013
    Date of Patent: January 31, 2017
    Inventors: Thomas W. Dubensky, Jr., Nancy A. Hosken, Scott H. Robbins, Margaret D. Moore
  • Patent number: 8853185
    Abstract: A monomer useful in prepa?ng therapeutic compounds includes a diversity element which potentially binds to a target molecule with a dissociation constant of less than 300 11 M and a linker element connected to the diversity element The linker element has a molecular weight less than 500 daltons, is connected, directly or indirectly through a connector, to said diversity element, and is capable of forming a reversible covalent bond or noncovalent interaction with a binding partner of the linker element The monomers can be covalently or non-covalently linked together to form a therapeutic multimer or a precursor thereof.
    Type: Grant
    Filed: April 9, 2009
    Date of Patent: October 7, 2014
    Assignees: Cornell University, Purdue Research Foundation
    Inventors: Francis Barany, Maneesh Pingle, Donald Bergstrom, Sarah Filippa Giardina
  • Patent number: 8815782
    Abstract: Provided herein is method comprising: contacting an initial RNA sample containing a population of different RNA molecules with a divalent cation and a set of DNAzymes that are designed to cleave multiple target RNAs in the initial sample, thereby producing a product RNA sample that comprises: a) uncleaved RNA molecules and b) cleaved RNA fragments that contain a 2?,3?-cyclic-phosphate and a 5? hydroxyl as the result of DNAzyme cleavage.
    Type: Grant
    Filed: November 1, 2012
    Date of Patent: August 26, 2014
    Assignee: Agilent Technologies, Inc.
    Inventors: Gusti Zeiner, Robert A. Ach
  • Publication number: 20140045707
    Abstract: Improved compositions, methods, apparatus, and kits for high-throughput nucleic acid amplification, detection and sequencing are disclosed. A nucleic acid cluster having an identifiable center is produced by generating on a solid support an immobilized nucleic acid complement from a template, one of which comprises a detectable label; and amplifying the complement and the template to obtain a nucleic acid cluster on the support, the cluster having a substantially central location marked by the detectable label and a surrounding region comprising immobilized copies. Also disclosed are nucleotide sequence determination in nucleic acid clusters so produced, center position annotation in the clusters, assignment of sequence information to overlapping clusters, and related compositions and methods.
    Type: Application
    Filed: September 6, 2013
    Publication date: February 13, 2014
    Applicant: Illumina Cambridge Limited
    Inventors: Alan D. Kersey, Jonathan Mark Boutell
  • Patent number: 8618024
    Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.
    Type: Grant
    Filed: August 31, 2006
    Date of Patent: December 31, 2013
    Assignee: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Publication number: 20130310417
    Abstract: The present invention provides small molecules for endothelial cell activation and compositions thereof and methods of making and using the same.
    Type: Application
    Filed: February 23, 2012
    Publication date: November 21, 2013
    Applicant: The Regents of the University fo California
    Inventors: Daniel Cruz, Ohyun Kwon
  • Patent number: 8575070
    Abstract: The present invention provides the means for producing libraries of peptide structures for drug screening applications that are capable of folding or assuming their native conformations independently of artificial scaffolds or flanking sequences in the proteins from which they are derived. The libraries can be highly diverse such that they are representative of the repertoire of protein structures existing in nature. The libraries can also be non-redundant or normalized such that the bias towards specific structures existing in source data sets and/or in nature is/are removed. In a particularly preferred embodiment, the present invention provides 30,000 independent fold structures produced by this method. The present invention also provides computer-readable media and systems comprising structural data in relation to the peptide libraries, and methods for displaying and screening the libraries.
    Type: Grant
    Filed: February 7, 2007
    Date of Patent: November 5, 2013
    Assignee: Phylogica Limited
    Inventors: Paul Michael Watt, Roland Dunbrack
  • Publication number: 20120190586
    Abstract: A method of making a synthetic organic receptor that specifically binds to a modified amino acid is carried out by: reacting a plurality of monomers in a dynamic combinatorial library in the presence of a protein or peptide to form a reaction product, the protein or peptide comprising a modified nucleic acid, with the reacting step carried out under conditions in which at least one oligomer that specifically binds to the modified amino acid is selectively amplified in the reaction product; and then isolating or identifying from the reaction product at least one oligomer that specifically binds to the modified amino acid.
    Type: Application
    Filed: October 13, 2010
    Publication date: July 26, 2012
    Inventors: Marcey L. Waters, Lindsey Ingerman James
  • Publication number: 20120015849
    Abstract: The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
    Type: Application
    Filed: June 7, 2011
    Publication date: January 19, 2012
    Applicant: DANISCO US INC.
    Inventors: Philippe Soucaille, Marguerite A. Cervin, Fernando Valle
  • Publication number: 20110281773
    Abstract: An integrated processing tool is described comprising a full-wafer processing module and a combinatorial processing module. Chemicals for use in the combinatorial processing module are fed from a delivery system including a set of first manifolds. An output of each first manifold is coupled to at least one mixing vessel. An output of each mixing vessel feeds more than one of a set of second manifolds. An output of each set of second manifolds feeds one of multiple site-isolated reactors of the combinatorial processing module.
    Type: Application
    Filed: July 25, 2011
    Publication date: November 17, 2011
    Inventors: Kurt H. Weiner, Tony P. Chiang, Aaron Francis, John Schmidt
  • Publication number: 20110224086
    Abstract: The present invention relates to methods and algorithms for identifying, synthesizing and co-assembling combinatorial libraries of polynucleotide variants.
    Type: Application
    Filed: March 9, 2010
    Publication date: September 15, 2011
    Inventors: Jose Pardinas, Shanrong Zhao
  • Patent number: 7972994
    Abstract: The present invention provides methods of synthesizing libraries of molecules comprising a functional moiety which is operatively linked to an encoding oligonucleotide, wherein the encoding oligonucleotide comprises a capping sequence containing degenerate nucleotides.
    Type: Grant
    Filed: June 9, 2006
    Date of Patent: July 5, 2011
    Assignee: GlaxoSmithKline LLC
    Inventors: Barry Morgan, Stephen Hale, Christopher C. Arico-Muendel, Matthew Clark, Richard Wagner, David I. Israel, Malcolm L. Gefter, Dennis Benjamin, Nils Jakob Vest Hansen, Malcolm J. Kavarana, Steffen Phillip Creaser, George J. Franklin, Paulo A. Centrella, Raksha A. Acharya
  • Publication number: 20100273660
    Abstract: In some aspects, the invention provides a genetically tractable in situ non-human animal model for hepatocellular carcinoma. The model is useful, inter alia, in understanding the molecular mechanisms of liver cancer, in understanding the genetic alterations that lead to chemoresistance or poor prognosis, and in identifying and evaluating new therapies against hepatocellular carcinomas. The liver cancer model of this invention is made by altering hepatocytes to increase oncogene expression, to reduce tumor suppressor gene expression or both and by transplanting the resulting hepatocytes into a recipient non-human animal. The present invention also provides methods for identifying and validating tumor suppressor genes by screening pools of shRNAs that target genomic regions deleted in human cancers, such as human hepatocellular carcinomas.
    Type: Application
    Filed: November 12, 2009
    Publication date: October 28, 2010
    Applicant: Cold Spring Harbor Laboratory
    Inventors: Lars Zender, Wen Xue, Scott Powers, Scott W. Lowe
  • Patent number: 7767627
    Abstract: Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials or, alternatively, allowing the components to interact to form at least two different materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, nonbiological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc.
    Type: Grant
    Filed: April 22, 1997
    Date of Patent: August 3, 2010
    Assignees: Symyx Solutions, Inc., The Regents of the University of California
    Inventors: Isy Goldwasser, Debra A. Ross, Peter G. Schultz, Xiao-Dong Xiang, Gabriel BriceƱo, Xian-Dong Sun, Kai-An Wang
  • Publication number: 20100184702
    Abstract: This invention provides novel polydentate selective high affinity ligands (SHALs) that can be used in a variety of applications in a manner analogous to the use of antibodies. SHALs typically comprise a multiplicity of ligands that each bind different region son the target molecule. The ligands are joined directly or through a linker thereby forming a polydentate moiety that typically binds the target molecule with high selectivity and avidity.
    Type: Application
    Filed: December 18, 2009
    Publication date: July 22, 2010
    Inventors: Sally DeNardo, Gerald DeNardo, Rodney Balhorn
  • Publication number: 20090285825
    Abstract: This invention is in the field of snake venom and the invention provides a novel snake toxin protein and nucleic acids encoding the same. Also, provided are various uses and compositions based on the discovery of the novel snake toxin.
    Type: Application
    Filed: December 22, 2005
    Publication date: November 19, 2009
    Applicant: National University of Singapore
    Inventors: Manjunatha Ramachandra Kini, Yuh Fen Fung, Prakash Pallathadka Kumar, Peter Tsun Hon Wong, Chung Pin Teo
  • Publication number: 20090018028
    Abstract: The present invention provides self-assembling, finite nucleic acid tiling arrays, and methods for their synthesis and use, which overcome a major hurdle in self-assembled DNA nanostructures, and therefore have numerous potential applications for nanofabrication of complex structures and useful devices, as further disclosed herein.
    Type: Application
    Filed: February 15, 2006
    Publication date: January 15, 2009
    Inventors: Stuart Lindsay, Hao Yan, John Chaput, Yan Liu, Peiming Zhang
  • Publication number: 20090011956
    Abstract: The present invention relates generally to programming of biomolecular self-assembly pathways and related methods and constructs. A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways for a wide variety of dynamic functions, reaction graphs for specifying pathways, and methods of using the hairpin motif are provided.
    Type: Application
    Filed: May 16, 2008
    Publication date: January 8, 2009
    Inventors: Peng Yin, Niles A. Pierce
  • Publication number: 20080070801
    Abstract: The present invention relates to methods and apparatus for synthesizing combinatorial materials libraries using pyrolysis techniques. In certain embodiments, the methods involve varying the precursors and/or reactant gases in an operating pyrolysis unit to continuously vary the resulting nanoparticle composition and collecting different nanoparticles at different locations on a substrate using a spatially addressable particle collector.
    Type: Application
    Filed: October 13, 2005
    Publication date: March 20, 2008
    Inventors: Xiao-Dong Xiang, Young K. Yoo, Yi-Qun Li, Daesig Kim, Sohrab Erfanian