Integrated Apparatus Specially Adapted For Creating A Library, Screening A Library, And Identifying A Library Member Patents (Class 506/34)
  • Patent number: 8357490
    Abstract: An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.
    Type: Grant
    Filed: July 22, 2010
    Date of Patent: January 22, 2013
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Thomas Froehlich, Martin Gutekunst, Dieter Heindl, Angelika Roesler, Rudolf Seibl
  • Patent number: 7994098
    Abstract: A prepared substrate upon which light directed DNA synthesis is to occur is exposed to light via an inverse mask pattern to deprotect inactive regions of the substrate where the synthesis is not intended to occur. The deprotected sites are then capped to disable permanently the inactive areas, thereby forming an inverse capped substrate. Unwanted DNA synthesis in the inactive areas is thus prevented, resulting in purer quality DNA, even though such areas may be exposed to light due to diffraction, scattering and flare during subsequent DNA synthesis of the intended active areas of the substrate.
    Type: Grant
    Filed: December 9, 2005
    Date of Patent: August 9, 2011
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Changhan Kim, Franco Cerrina
  • Patent number: 7989396
    Abstract: A highly specific and versatile surface chemistry for immobilization of amine-terminated probes is disclosed. A bi-layered polymer thin film serves as the platform for coupling the probes, which are preferably oligonucleotides. The process involves sequentially coating a substrate with polyamine and polyacid anhydride. Hydrolyzed polyacid anhydride groups may be converted to non-hydrolyzed groups at about 100° C. prior to probe attachment. The process of coating the substrate requires no harsh chemical pretreatment of substrates such as RCA or Piranha cleaning. In addition, simple thermal activation of the anhydride groups has a low requirement for storage, leading to a long shelf life of modified surfaces. The disclosed surface chemistry is especially compatible with microfabrication processes, and its effective application to magnetic biosensors is demonstrated.
    Type: Grant
    Filed: December 3, 2007
    Date of Patent: August 2, 2011
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Heng Yu, Nader Pourmand, Shan X. Wang
  • Publication number: 20110136698
    Abstract: A chip with tri-layer electrodes and micro-cavity arrays for control of bioparticles and a manufacturing method thereof are revealed. The chip captures and releases bioparticles into and from preset cavities by dielectrophoresis (DEP) force generated by electrodes. The chip includes an upper layer body, a middle layer body, a lower layer body, respectively disposed with an electrode, and micro flow chambers. The electrodes of the upper layer body and the middle layer body are common electrodes while the electrode of the lower layer body is a dispersive electrode array exposed on the bottom of lower-layer microcavity. The cell capture and release at the single-cell level and the cell population level are attained by application of an AC electric field.
    Type: Application
    Filed: December 7, 2009
    Publication date: June 9, 2011
    Inventors: Cheng-Hsin Chuang, You-Ming Hsu, Yao-Tung Wu
  • Patent number: 7576037
    Abstract: Apparatus and methods are described for split synthesis combinatorial chemistry that provides candidate libraries where an even distribution of theoretical products is obtainable through even mixing during the pooling step, followed by controlled redistribution of the mixed pooled products from the prior addition step into separate synthesis columns, one for each different specie of subunit to be added.
    Type: Grant
    Filed: November 18, 2005
    Date of Patent: August 18, 2009
    Assignees: MEI Technologies, Inc., Bd. of Regents, Univ. of Texas
    Inventors: Johnnie Paul Engelhardt, David G. Gorenstein, Bruce A. Luxon
  • Publication number: 20080269063
    Abstract: System and method for preparing a microarray for a disease association gene transcript test. Disease considerations for this unique test include a custom set of genetic sequences associated in peer-reviewed literature with various known diseases such as Addison's disease, anemia, asthma, atherosclerosis, autism, breast cancer, estrogen metabolism, Grave's disease, hormone replacement therapy, major histocompatibility complex (MHC) genes, longevity, lupus, multiple sclerosis, obesity, osteoarthritis, prostate cancer, and type 2 diabetes. The base dataset may be developed through clinical samples obtained by third-parties. Online access of real-time phenotype/genotype associative testing for physicians and patients may be promoted through an analysis of a customized microarray testing service.
    Type: Application
    Filed: July 10, 2007
    Publication date: October 30, 2008
    Applicant: IGD INTEL, LLC
    Inventor: Dean Iverson Sproles