For Screening A Library Patents (Class 506/39)
  • Patent number: 10295544
    Abstract: This disclosure provides high throughput immunoblot methods and apparatus for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The method yields highly sensitive and accurate results and is useful in analyze complex system including an antigen from cell or tissue lysate.
    Type: Grant
    Filed: February 15, 2017
    Date of Patent: May 21, 2019
    Inventor: Jiandi Zhang
  • Patent number: 10254268
    Abstract: Disclosed is a method for detecting toxic metal ions in a sample. The method includes: a) preparing a solution of organic acid-bound gold nanoparticles; b) adding a sample containing toxic metal ions to the solution prepared in a) to allow the gold nanoparticles to aggregate; c) dropping the reaction solution obtained in b) onto a silicon substrate and drying the reaction solution such that the gold nanoparticle aggregates are immobilized on the silicon substrate; and d) analyzing the characteristics of the gold nanoparticles immobilized on the silicon substrate. The method enables the detection of even a trace amount of toxic metal ions in a sample with high sensitivity. Therefore, the method can be applied to the management of water quality in food service providers and hospitals, the measurement of contaminants in water supply and drainage systems, and the management of industrial wastewater.
    Type: Grant
    Filed: March 21, 2017
    Date of Patent: April 9, 2019
    Assignee: Korea University Research and Business Foundation, Sejong Campus
    Inventors: Jinsung Park, Woong Kim, Joohyung Park, Seongjae Jo, Minwoo Kim, Gyudo Lee
  • Patent number: 10168346
    Abstract: A method for determining the presence or absence of disposable pipette tips in pipette tip carriers on the work area of a laboratory workstation. Each of the pipette tip carriers has a support panel with receiving holes into each of which a disposable pipette tip can be inserted. The laboratory workstation for carrying out the method has a robot arm with at least one pipette which is designed to receive and dispose of disposable pipette tips. The laboratory workstation has a digital camera which is arranged on a support device and is operatively connected to an analyzing unit. The work area of the laboratory workstation can be completely imaged in at least one first direction using the digital camera.
    Type: Grant
    Filed: April 17, 2015
    Date of Patent: January 1, 2019
    Assignee: TECAN TRADING AG
    Inventors: Martin Schmidt, Rainer Kerkmann
  • Patent number: 10023897
    Abstract: A biosensor chip is placed in a biosensor device and is rotated while a biochemical analysis specimen is measured, the biosensor chip comprising a main body, a holding chamber, a dispensing chamber, a plurality of quantification chambers, and a plurality of measurement chambers. The main body has an inlet into which the specimen is poured. The holding chamber holds the poured specimen inside the main body. The dispensing chamber is connected to the holding chamber via a first channel and dispenses the specimen. The quantification chambers are connected to the dispensing chamber, hold a specific amount of the dispensed specimen, and are disposed at positions located away from a rotational center of a rotary motion according to a distance from the first channel. The measurement chambers are connected to the quantification chambers via a second channel and react the specimen with a biochemical analysis reagent.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: July 17, 2018
    Inventors: Kazuyoshi Mori, Masahiro Kouge, Fumihisa Kitawaki, Takako Matsumura, Seiji Onishi
  • Patent number: 9976174
    Abstract: Method includes positioning a first carrier assembly on a system stage. The carrier assembly includes a support frame having an inner frame edge that defines a window of the support frame. The first carrier assembly includes a first substrate that is positioned within the window and surrounded by the inner frame edge. The first substrate has a sample thereon. The method includes detecting optical signals from the sample of the first substrate. The method also includes replacing the first carrier assembly on the system stage with a second carrier assembly on the system stage. The second carrier assembly includes the support frame and an adapter plate held by the support frame. The second carrier assembly has a second substrate held by the adapter plate that has a sample thereon. The method also includes detecting optical signals from the sample of the second substrate.
    Type: Grant
    Filed: March 22, 2016
    Date of Patent: May 22, 2018
    Inventors: Stephen Rawlings, Venkatesh Mysore Nagaraja Rao, Beng Keong Ang, Nitin Udpa
  • Patent number: 9869658
    Abstract: A technique for electrical detection of a molecule is provided. A fluidic bridge is formed between a nanopipette and a fluid cell, where the molecule is in the nanopipette. A voltage difference is applied between the nanopipette and the fluid cell, where the fluid cell contains an electrolyte solution. Entry of the molecule into the fluidic bridge is determined by detecting a fore pulse. The fluidic bridge between the nanopipette and the fluid cell is broken to form a nanogap. In response to waiting a time interval, the fluidic bridge is reformed between the nanopipette and the fluid cell to close the nanogap. The molecule is determined to exit the nanopipette by detecting an after pulse.
    Type: Grant
    Filed: July 22, 2014
    Date of Patent: January 16, 2018
    Inventors: Venkat K. Balagurusamy, Stanislav Polonsky
  • Patent number: 9796748
    Abstract: The invention provides systems and methods for spatial sequestration of elements in nucleic acid circuits.
    Type: Grant
    Filed: May 2, 2012
    Date of Patent: October 24, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Thomas E. Schaus, David Yu Zhang, Wei Sun, Peng Yin
  • Patent number: 9643178
    Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyzes. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyzes requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyzes.
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: May 9, 2017
    Assignee: Fluidigm Corporation
    Inventors: Marc Unger, Ian D. Manger, Michael Lucero, Yong Yi, Emily Miyashita-Lin, Anja Wienecke, Geoffrey Facer
  • Patent number: 9513224
    Abstract: Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.
    Type: Grant
    Filed: January 22, 2014
    Date of Patent: December 6, 2016
    Assignee: Theranos, Inc.
    Inventors: Karan Mohan, Chinmay Pangarkar, James Wasson
  • Patent number: 9494521
    Abstract: Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.
    Type: Grant
    Filed: July 25, 2013
    Date of Patent: November 15, 2016
    Assignee: Theranos, Inc.
    Inventors: Elizabeth A. Holmes, Chinmay Pangarkar, Timothy Smith, Karan Mohan, Samartha Anekal, Daniel L. Young, James R. Wasson
  • Patent number: 9428801
    Abstract: The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.
    Type: Grant
    Filed: December 29, 2011
    Date of Patent: August 30, 2016
    Inventors: Mark Eshoo, Stanley Motley, John Picuri
  • Patent number: 9399214
    Abstract: A cartridge includes a first drum having a first chamber and a displacement device that is configured to rotate the first drum about a central axis to connect the first chamber to a second chamber in a conductive manner. The cartridge further includes an electric switch that is configured to be actuated by the displacement device between a closed switching state and an open switching state.
    Type: Grant
    Filed: June 7, 2012
    Date of Patent: July 26, 2016
    Assignee: Robert Bosch GmbH
    Inventors: Martina Daub, Juergen Steigert
  • Patent number: 9376713
    Abstract: Provided are methods and devices for label-free detection of nucleic acids that are amplified by polymerase chain reaction. A solution containing the components necessary for a PCR is introduced to a microfluidic amplification chamber and an electric field applied to a confined region in which PCR occurs. PCR product generated in the confined region is detected by measuring an electrical parameter that is, for example, solution impedance. The devices and methods provided herein are used, for example, in assays to detect one or more pathogens or for point-of-care tests. In an aspect, the PCR product is confined to droplets and the assay relates to detecting an electrical parameter of a flowing droplet, thereby detecting PCR product without a label. In an aspect, the PCR occurs in the droplet.
    Type: Grant
    Filed: September 23, 2010
    Date of Patent: June 28, 2016
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Rashid Bashir, Yi-Shao Liu, Eric Salm, Woo-Jin Chang, Nicholas N. Watkins
  • Patent number: 9290798
    Abstract: The invention provides methods to process multiple microarrays by providing a microarray plate and processing plates. In an embodiment of the invention, methods for assembling microarray plates by using wafers are described for high throughput microarray processing.
    Type: Grant
    Filed: July 2, 2013
    Date of Patent: March 22, 2016
    Assignee: Affymetrix, Inc.
    Inventor: Mohsen Shirazi
  • Patent number: 9116139
    Abstract: A technique is disclosed for sample management for use in conjunction with sequencing devices that sequence biological samples, e.g., DNA and RNA. A sequencing device or a network of sequencing devices may be scheduled according to the characteristics of the samples in queue and the capabilities and availability of sequencing devices. Biological samples may be automatically queued and loaded via a sample distribution system. A sample distribution system may be used to reduce operator intervention.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: August 25, 2015
    Assignee: ILLUMINA, INC.
    Inventors: Robert C. Kain, Alexander G. Dickinson, Min-Jui Richard Shen, Helmy A. Eltoukhy, Francisco Jose Garcia
  • Patent number: 9064965
    Abstract: This application discloses ZnO film transistor-based immunosensors (ZnO-bioTFT), 2T biosensor arrays formed from two integrated ZnO-bioTFTs, 1T1R-based nonvolatile memory (NVM) arrays formed from ZnO-bioTFT (T) integrated with ZnO-based resistive switches (R), as well as integrated bioTFT (IBTFT) sensor systems formed from 2T biosensor arrays and 1T1R NVM arrays. Through biofunctionalization, these biosensors can perform immunosensing with high sensitivity and selectivity, and therefore have a wide range of applications, for example, in detecting target biomolecules or small molecules, and potential application in cancer diagnosis and treatment.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: June 23, 2015
    Inventors: Yicheng Lu, Pavel Ivanoff Reyes, Ki-Bum Lee, Aniruddh Solanki, Chieh-Jen Ku
  • Publication number: 20150148258
    Abstract: The present invention describes a method and devices for the simultaneous detection, recovery identification and counting of a plurality of microorganisms consisting in providing mixtures of nutrients specially selected from those that curtail the lag phase of growth in bacteria and moulds and which, together with fluorescent enzymatic, chromogenic or bioluminescent markers and other nutrient components or growth inhibitors, are embedded in three-dimensional structures or natural or artificial clays or ceramics with cavities of different dimensions and forms and specific surface areas of between 2×103 and 6×108 m2/m3.
    Type: Application
    Filed: March 27, 2013
    Publication date: May 28, 2015
    Inventors: Claudio Rodriguez Martinez, Jesus Eduardo Gonzalez Ruiz, Tamara Lobaina Rodriguez, Raisa Zhurbenko, Ana Iris Brito Gonzales, Monica Lopez Hernandez, Javier Fernandez Aragon, Ivonne Alfonso Valdes, Adelaida Ortea Suris
  • Publication number: 20150148265
    Abstract: Devices and systems for sampling and profiling microbiota of skin are described which include a replaceable microbe sampling unit including a substrate and a location information storage component, the substrate including a microbe-capture region configured to capture at least one type of microbe from one or more regions of a skin surface of an individual, and the location information storage component configured to store information associated with the location of said one or more regions of the skin surface of the individual.
    Type: Application
    Filed: November 27, 2013
    Publication date: May 28, 2015
    Inventors: Michael H. Baym, Roderick A. Hyde, Jordin T. Kare, Eric C. Leuthardt, Gary L. McKnight, Tony S. Pan, Elizabeth A. Sweeney, Clarence T. Tegreene, Lowell L. Wood, JR.
  • Publication number: 20150148242
    Abstract: The present application is directed to methods and systems for identifying small molecule compounds in mixtures using a library comprising calculated structures and corresponding calculated mass spectral fragmentation patterns of known and/or hypothetical small molecule compounds that may be in the mixture and screening of a mass spectrum of the mixture using the library to identify matching fragmentation patterns. If a mass spectral fragmentation pattern present in the mass spectrum of the mixture matches a calculated fragmentation pattern of one of the known or hypothetical compounds this confirms the identity of a compound in the mixture as the known or hypothetical compound. The method represents a platform method that can be used for a multitude of purposes related to the screening and identification of compounds in mixtures.
    Type: Application
    Filed: June 5, 2013
    Publication date: May 28, 2015
    Applicant: McMaster University
    Inventors: Nathan Magarvey, Aubrey Bailey Morgan Wyatt, Chad William Johnston, Ashraf Ibrahim, Bin Ma, Lian Yang
  • Patent number: 9040463
    Abstract: The invention provides a multiplexed assay device comprising a reaction chamber and several sets of encoded microcarriers 2 wherein the reaction chamber is a microchannel 1 and wherein the longitudinal movement of the microcarriers 2 is restricted and wherein the microcarriers 2 have a shape in relation to the geometry of the microchannel 1 such that at least two can stand side by side in the microchannel 1 without touching each other and without touching the perimeter of the microchannel 1 and are preferably observable in the reaction chamber. Moreover, the invention provides a method for performing multiplexed assay based on microcarriers 2 that improves mass transfer, simplifies the preparation and the execution of the assay and facilitates readout of biological reactions and identity of microcarriers 2.
    Type: Grant
    Filed: December 23, 2009
    Date of Patent: May 26, 2015
    Assignee: MYCARTIS NV
    Inventors: Nicolas Demierre, Nader Donzel, Jose Gil, Philippe Renaud
  • Publication number: 20150141264
    Abstract: The invention provides a method for in-field detection of a distinctive marker. The method includes providing a sample from an article of interest and analyzing the sample to detect the presence of the distinctive marker. The analysis is performed using an in-field detection instrument. The in-field detection instrument includes a microsystem configured to perform sample in-answer out analysis and detect the presence of the distinctive marker in the sample.
    Type: Application
    Filed: August 28, 2014
    Publication date: May 21, 2015
    Inventors: Lawrence Jung, MingHwa Benjamin Liang
  • Publication number: 20150141281
    Abstract: The present invention relates to a method for performing a chemical and/or a biological assay comprising the following successive steps of: a) providing an assay device with a microchannel having an inlet and an outlet and further comprising restricting means designed to restrict the movement toward the outlet of microparticles introduced in the microchannel while letting a fluid to flow through the restricting means, b) introducing microparticles in the microchannel via the inlet, c) restricting the movement of said microparticles in the microchannel toward the outlet using restricting means, d) flowing a fluid sample through the microchannel, e) performing a biological and/or chemical read-out on each microparticle, the method further comprising the steps of: f) moving the microparticles in the microchannel, and g) repeating successively the steps d) and e).
    Type: Application
    Filed: July 10, 2013
    Publication date: May 21, 2015
    Applicant: MYCARTIS NV
    Inventors: Raphael Tornay, Nicolas Demierre, Jose De Gil, Robin Muller, Didier Falconnet
  • Publication number: 20150133345
    Abstract: The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.
    Type: Application
    Filed: November 10, 2014
    Publication date: May 14, 2015
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra
  • Publication number: 20150133321
    Abstract: The present disclosure relates to characterization of biological samples. By way of example, a biological sample may be contacted with a plurality of probes specific for targets in the sample, such as probes for immune markers and segmenting probes. Acquired image data of the sample may be used to segment the images into epithelial and stromal regions to characterize individual cells in the sample based on the binding of the probes. Further, the biological sample may be characterized by a distribution, location, and type of a plurality of the characterized cells.
    Type: Application
    Filed: November 13, 2013
    Publication date: May 14, 2015
    Applicant: General Electric Company
    Inventors: Srabani Bhaumik, Michael John Gerdes, Zhengyu Pang, Fiona Ginty, Christopher James Sevinsky, JR., Qing Li, Alberto Santamaria-Pang, Yunxia Sui, Keyur Hemantkumar Desai
  • Publication number: 20150126404
    Abstract: A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.
    Type: Application
    Filed: November 5, 2014
    Publication date: May 7, 2015
    Inventors: Yanqing Tian, Liqiang Zhang, Fengyu Su, Deirdre Meldrum, Sean Buizer, Clifford L. Anderson, Laimonas Kelbauskas, Kristen Lee
  • Publication number: 20150126409
    Abstract: The present invention relates to the detection of microbial organisms using non-toxic nanostructured sensors that change their physical or chemical properties upon detecting these microbial organisms. These sensors allow an unskilled person to rapidly detect the presence of microbial contamination.
    Type: Application
    Filed: November 3, 2014
    Publication date: May 7, 2015
    Inventors: Khashayar Ghandi, Zahid Mahimwalla, Tan Yang, Cody Landry
  • Publication number: 20150119280
    Abstract: Techniques for hydrogel microstructures with oil isolation for small reaction volumes include providing a hydrogel microstructure that has a plurality of pores and a hydrogel frame surrounding the pores. The microstructure has a volume in a range from about 1 picoliter to about 10,000 picoliters and is configured to repel an immiscible fluid. Each pore of the plurality of pores has a pore size configured to pass the target molecule in a first solution. The microstructure is contacted with the first solution, and with a second solution that includes a reactant molecule that reacts with the target molecule to produce an observable product molecule. The microstructure is encompassed with the immiscible fluid for an extended observation duration from about 1 to about 10,000 seconds, wherein the immiscible fluid does not pass into the pores but traps the product in the microstructure. The observable product molecule is measured at some time during the observation duration.
    Type: Application
    Filed: October 27, 2014
    Publication date: April 30, 2015
    Applicant: Massachusetts Institute of Technology
    Inventors: Rathi Lakshmi Srinivas, Patrick S. Doyle
  • Publication number: 20150119296
    Abstract: Novel and improved systems and methods for high speed arraying, hybridization, quantitative development and/or assaying are provided. Some embodiments provide a web based arraying format. Some other embodiments provide a sheet based arraying format. Some embodiments use a drop on drop assaying or hybridization mode. In some embodiments, a substantially inert substrate is utilized. In some other embodiments, an interactive substrate is utilized.
    Type: Application
    Filed: December 23, 2014
    Publication date: April 30, 2015
    Inventors: Thomas C. Tisone, Holger Eickhoff
  • Publication number: 20150119298
    Abstract: A system for performing one or more microfluidic processes includes an integrated fluidic device comprising a plurality of well regions and a plurality of control valves and a workflow manager. The system also includes a transfer robot adapted to transfer the integrated fluidic device between a plurality of stations in response to a series of instructions from the workflow manager and a first station comprising a dispensing robot adapted to dispense at least one of a plurality of sample solutions and at least one of a plurality of reagents into the integrated fluidic device. The system further includes a second station comprising a fluidic controller unit and a third station comprising an inspection station.
    Type: Application
    Filed: September 29, 2014
    Publication date: April 30, 2015
    Inventors: Gang Sun, Greg Harris, Andy May, Kyle Self, Kevin Farrell, Paul Wyatt
  • Publication number: 20150119274
    Abstract: The present invention describes microplates with arrays of wells containing magnetic inserts in the form of disks, cylinders or otherwise shaped materials with apertures in the centre for optical interrogation of the contacting solution. These inserts are capable of capturing ferro- and paramagnetic nano- and microparticles. The subject microplates find use in a variety of applications, including clinical and environmental assays, high throughput screening for genomics, proteomics and pharmaceutical applications, point-of-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally and high-throughput screening of materials for separation and catalysis.
    Type: Application
    Filed: June 3, 2013
    Publication date: April 30, 2015
    Inventors: Sergey Anatoliyovich Piletsky, Olena Piletska, Antonio Guerreiro, Alessandro Poma, Khalku Karim, Stanislav Piletsky
  • Publication number: 20150119262
    Abstract: This disclosure relates generally to the prediction of a gene target of an enhancer based on genomic proximity and a comparison to a set of known enhancer-gene pairs. A gene can be selected a gene from a given cell type. A domain can be established bi-directionally around the gene, and enhancers located within the domain can be identified. The enhancers located within the domain can be considered to be in close genomic proximity to the gene. For each enhancer, a test pair of the enhancer and the gene can be established and compared to a set of known enhancer-gene pairs. The prediction can be established based on the comparison and the genomic proximity.
    Type: Application
    Filed: October 27, 2014
    Publication date: April 30, 2015
    Inventor: Peter C/ Scacheri
  • Publication number: 20150119286
    Abstract: A high-throughput corrosion testing mechanism was developed for metals in a variety of environments in controlled, multiplexed microenvironments. Many parallel experiments can be conducted with microbial and environmental variables independently manipulated to identify the key determinants of corrosion progression. The synthetic assay design enables subsequent surface characterization of select samples within the array. In as little as one day, diverse corrosive environments can be compared quantitatively.
    Type: Application
    Filed: October 29, 2014
    Publication date: April 30, 2015
    Inventors: Matthew James Whitfield, Krystyn J. Van Viet
  • Publication number: 20150119297
    Abstract: There are systems and methods for performing an assay to generate genotype information about a subject associated with a medical condition. There are also systems and method for generating and utilizing prognostic information associated with treating the patient with a medication based on the genotype information and an association of the genotype and metabolizing the medication. The genotype information includes data relating to SNP alleles in the patient's genotype and the association of the alleles and metabolism of a medication by the patient.
    Type: Application
    Filed: October 28, 2013
    Publication date: April 30, 2015
    Inventor: Brian Meshkin
  • Publication number: 20150119268
    Abstract: A biological sample processing apparatus having an enclosure. A plurality of sample processing modules are held by the enclosure. Each sample processing module is configured to hold a removable sample cartridge and to only perform sample processing on a sample within the corresponding removable sample cartridge. Each sample processing module is configured to perform at least one of a plurality of testing processes on the sample within the removable sample cartridge. At least one module in the apparatus is configured to perform nucleic acid amplification and detection.
    Type: Application
    Filed: April 25, 2013
    Publication date: April 30, 2015
    Applicant: CEPHEID
    Inventor: John L. Bishop
  • Publication number: 20150111790
    Abstract: The invention is a device for testing an individual for the presence of a entity. The entity can be a pathogen, toxin or drug. The device is adapted to collect from the individual the biological sample to be tested. The test device comprises noble metal nanoparticles (i.e. gold, silver or copper) to detect the entity. Test results are read from a window in the device. The current visual limit using aggregated noble metal nanoparticles is a clump having a diameter in a range of about 50 nm to about 75 nm. One or more ligands specific for the entity are attached to two or more metal nanoparticles. Each individual nanoparticle has a diameter in a range of about 15 nm to about 35 nm. Ligands attached to the nanoparticles and specific for the entity can be for example antibodies, antibody fragments, oligonucleotides, and aptamers. Different ligands specific for different sites on the entity may be used in order to increase the aggregation potential of the nanoparticles in the presence of the target.
    Type: Application
    Filed: January 30, 2014
    Publication date: April 23, 2015
    Inventors: Christopher Ategeka, Anwaar Al-Zireeni
  • Publication number: 20150111770
    Abstract: This invention relates to determination of quantitative expression of plasma membrane proteins as biomarkers in the erythrocytes. The invention includes simple, quantitative assay platforms which can be made available in most diagnostic laboratories. The platform allows performing personalized, quantitative tests for the direct expression level of a wide range of membrane proteins from small volume blood samples and connecting them to individual genetic variability, disease conditions, disease stages and complications, treatment protocols, pharmacological responses, or toxic side effects.
    Type: Application
    Filed: April 22, 2013
    Publication date: April 23, 2015
    Applicant: AdvanCell Diagnosztika Kft.
    Inventors: Balázs Sarkadi, György Várady, Ildikó Kasza
  • Publication number: 20150111765
    Abstract: Biosensor components (chips) are described based on direct biocoating processes that result in the tenacious and stable, noncovalent (believed to be chemisorptive) binding of anchor substances such as avidin(s) other proteins having specific binding partners or oligo- or poly-nucleotides onto any piezo-electrically active crystal surface. The resulting platform technology can be developed for a variety of biosensors with specific applications in biological assays. The table mono layers of the anchor substances forms reactive layers, ready to bind a capture reagent such as a biot-inylated antibody for capture and detection of analytes in biologic fluid samples.
    Type: Application
    Filed: August 27, 2012
    Publication date: April 23, 2015
    Inventors: Lisa Laury-Kleintop, Herman Rutner
  • Patent number: 9012374
    Abstract: The methods of the present invention provide methods for manufacturing a master substrate and methods for manufacturing replica arrays from the master substrate. The methods may be used, for example, directly to manufacture or “print” peptide arrays from a DNA array; however, the methods are applicable to a wide range of manufacturing applications for use any time multiple copies of an array needs to be printed.
    Type: Grant
    Filed: December 3, 2012
    Date of Patent: April 21, 2015
    Assignee: Prognosys Biosciences, Inc.
    Inventor: David A. Routenberg
  • Publication number: 20150105292
    Abstract: A method for analyzing a binding ability of protein to a compound, involves fractionating first and second groups of proteins into plural fractions using a carrier having the compound immobilized thereon; combining fractions, analyzing the combined fractions with mass spectrometry; based on the mass spectrometry information, obtaining, regarding each fraction, an intensity ratio between peaks derived from a protein in each of the groups of fractions; and comparing degrees of the binding ability of the plural kinds of proteins to the compound.
    Type: Application
    Filed: October 17, 2014
    Publication date: April 16, 2015
    Inventors: Yoshiya Oda, Hiroyuki Katayama
  • Publication number: 20150105295
    Abstract: Analysis of a system and/or sample involves the use of absorption-encoded micro beads. Each type of micro bead is encoded with amounts of the k dyes in a proportional relationship that is different from proportional relationships of the k dyes of others of the n types of absorption-encoded micro beads. A system and/or a sample can be analyzed using information obtained from detecting the one or more types of absorption-encoded micro beads.
    Type: Application
    Filed: December 18, 2014
    Publication date: April 16, 2015
    Inventors: Peter Kiesel, Joerg Martini, Michael I. Recht
  • Publication number: 20150105297
    Abstract: A micro-fluidic device for mapping a DNA or RNA strand labeled at a plurality of specific sites with labels suitable for generating a detection signal when interacting with a detector element, the device comprising: a micro-fluidic channel; and a plurality of detector elements for detecting the labels by acquiring the detection signals, the detector elements being positioned longitudinally along the micro-fluidic channel, each detector element having a width, successive detector elements being separated by an inter-detector gap having a width, wherein the widths of at least two of the detector elements are different and/or wherein the widths at least two of the inter-detector gaps are different.
    Type: Application
    Filed: October 13, 2014
    Publication date: April 16, 2015
    Applicants: Katholieke Universiteit Leuven, KU LEUVEN R&D, IMEC VZW
    Inventors: Tim Stakenborg, Robert Neely, Pol Van Dorpe, Johan Hofkens
  • Publication number: 20150099655
    Abstract: Preeclampsia markers, preeclampsia marker panels, and methods for obtaining a preeclampsia marker level representation for a sample are provided. These compositions and methods find use in a number of applications, including, for example, diagnosing preeclampsia, prognosing a preeclampsia, monitoring a subject with preeclampsia, and determining a treatment for preeclampsia. In addition, systems, devices and kits thereof that find use in practicing the subject methods are provided.
    Type: Application
    Filed: May 7, 2013
    Publication date: April 9, 2015
    Inventors: Atul J. Butte, Bruce Xuefeng Ling, Linda Liu Miller, Alexander A. Morgan, Gongxing Chen, Jun Ji, Ting Yang
  • Publication number: 20150099660
    Abstract: Microfluidic devices and methods for using the same are provided. Aspects of the invention include microfluidic devices that include a separation medium and a pan-capture binding medium. The microfluidic devices are configured to subject a sample to two or more directionally distinct electric fields. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.
    Type: Application
    Filed: November 7, 2014
    Publication date: April 9, 2015
    Inventors: Amy E. Herr, Dohyun Kim
  • Publication number: 20150099644
    Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.
    Type: Application
    Filed: April 19, 2013
    Publication date: April 9, 2015
    Inventors: Caifu Chen, Casey McFarland, David Keys
  • Publication number: 20150094223
    Abstract: A method and apparatus for diagnosing cancer by using genetic information, the method comprising acquiring first gene expression data of a subject, for whom cancer is to be diagnosed, for a gene marker set including at least one gene marker; and determining a possibility of a presence of the cancer of the subject by using the acquired first gene expression data and pre-stored second gene expression data of a normal person group and a cancer patient group, wherein the gene marker set includes gene markers such as pyrroline-5-carboxylate reductase 1 (PYCR1), phosphoglycerate dehydrogenase (PHGDH), glutaminase 2 (liver, mitochondrial) (GLS2), and glutaminase (GLS) among others.
    Type: Application
    Filed: September 3, 2014
    Publication date: April 2, 2015
    Inventors: Eunjin Lee, Taejin Ahn
  • Publication number: 20150094227
    Abstract: Disclosed is a test device to detect pregnancy in a human female subject, the test device comprising: an assay means to measure the absolute or relative amount of hCG in a sample from the subject; an assay means to measure the absolute or relative amount of FSH in a sample from the subject; and an assay means to measure the absolute or relative amount of one or more progesterone metabolites in a sample from the subject.
    Type: Application
    Filed: October 2, 2014
    Publication date: April 2, 2015
    Inventors: David McCarthy, Saji Eapen
  • Patent number: 8993487
    Abstract: A bio-disc device including new valve control means and fluid movement system, a bio-driver apparatus in which a controller disc including a controller for the bio-disc is installed, and an assay method using the same, which are suitable for labs-on-a-chips for various diagnostic assays, nucleic acid hybridization assays, and immunoassays, are provided. The bio-driver apparatus is compatible with general optical discs, including audio CDs, CD-Rs, game CDs, DVDs, etc., and the assay method is compatible with general optical disc drivers, including CD-ROMs, DVD players, etc. Thus, the bio-driver apparatus and the assay method offer and economical and convenient alternative to existing products. In addition, the bio-driver apparatus can be readily and easily applied in connection with a computer for remote diagnosis via the Internet.
    Type: Grant
    Filed: May 2, 2006
    Date of Patent: March 31, 2015
    Assignee: Samsung Electronics Co., Ltd.
    Inventor: Jae Chern Yoo
  • Patent number: 8992860
    Abstract: The present invention relates to systems and methods for minimizing or eliminating diffusion effects. Diffused regions of a segmented flow of multiple, miscible fluid species may be vented off to a waste channel, and non-diffused regions of fluid may be preferentially pulled off the channel that contains the segmented flow. Multiple fluid samples that are not contaminated via diffusion may be collected for analysis and measurement in a single channel. The systems and methods for minimizing or eliminating diffusion effects may be used to minimize or eliminate diffusion effects in a microfluidic system for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.
    Type: Grant
    Filed: April 14, 2014
    Date of Patent: March 31, 2015
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Brian Murphy, Scott Corey, Alex Flamm, Ben Lane, Conrad Laskowski, Chad Schneider
  • Publication number: 20150087559
    Abstract: A microfluidic device comprising a microfluidic channel network sealed on one side by a membrane sheet, the sheet having PDMS defining at least the surface sealing the channel, the membrane sheet on its opposite side sealing one side of a pneumatic channel, the pneumatic channel arranged to enable pneumatic deflection of a deflectable portion of the membrane sheet into contact with an opposed surface to control flow in a channel of the network, the membrane sheet confining in a channel of the network at least one micro-particle, micro-length tube or glass nano reactor, functionalized with a capture agent, that has been inserted into that channel. A microfluidic device having a microfluidic channel containing at least two micro-particles, micro-length tubes or glass nano reactors, one functionalized with nucleic acid and another with antibody or antigen.
    Type: Application
    Filed: September 6, 2014
    Publication date: March 26, 2015
    Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
  • Publication number: 20150087551
    Abstract: Apparatus, systems, and methods for detecting, screening and sampling of cells are disclosed.
    Type: Application
    Filed: July 22, 2014
    Publication date: March 26, 2015
    Inventor: Rolf U. Halden