For Screening A Library Patents (Class 506/39)
  • Patent number: 11125939
    Abstract: A waveguide filter sensing unit is provided. The waveguide sensing unit includes an input waveguide for receiving an optical signal and an interference waveguide region for filtering the optical signal to remove noise therein. The waveguide sensing unit further includes a cladding layer wrapping around the input waveguide and the interference waveguide region; and an optical signal detector converting the filtered optical signal into an electrical signal. The width of the input waveguide is smaller than that of the interference waveguide region, and the refractive index of the cladding layer is smaller than that of the input waveguide and the interference waveguide region.
    Type: Grant
    Filed: April 6, 2021
    Date of Patent: September 21, 2021
    Assignee: GeneSense Technology Inc.
    Inventors: Bingzhou Hong, Xianchao Wang, Haochen Cui, Tianshu Wang, Yinghua Sun, Mei Yan
  • Patent number: 11092562
    Abstract: Embodiments of the present disclosure pertain to conductive textiles that include a textile component with a plurality of fibers; and metal-organic frameworks associated with the fibers of the textile component in the form of a conductive network. Metal-organic frameworks may have a two-dimensional structure and a crystalline form. Metal-organic frameworks may be conformally coated on the fibers of the textile component. Additional embodiments of the present disclosure pertain to methods of sensing an analyte in a sample by exposing the sample to a conductive textile; and detecting the presence or absence of the analyte by detecting a change in a property of the conductive textile, and correlating the change in the property to the presence or absence of the analyte. The analyte in the sample may reversibly associate with the conductive textile. The association may also result in filtration, pre-concentration, and capture of the analyte by the conductive textile.
    Type: Grant
    Filed: April 25, 2018
    Date of Patent: August 17, 2021
    Assignee: TRUSTEES OF DARTMOUTH COLLEGE
    Inventors: Katherine A. Mirica, Merry K. Smith
  • Patent number: 10988799
    Abstract: An analysis system for testing a biological sample wherein a plurality of assays selected from at least two assays from a group formed of a protein assay for detecting a target protein, a nucleic-acid assay for detecting a target nucleic-acid sequence and/or an aptamer assay for detecting another target analyte. The testing is carried out sequentially in a common sensor array by means of a sensor apparatus.
    Type: Grant
    Filed: February 7, 2020
    Date of Patent: April 27, 2021
    Assignee: BOEHRINGER INGELHEIM VETMEDICA GMBH
    Inventors: Matthias Griessner, Heinz Schoeder
  • Patent number: 10969337
    Abstract: A method and a system for rapid kinetic fluorescence measurement in high-throughput screening with high time-resolution includes providing a microtiter plate having a transparent base and multiple assay wells, a dispensing system arranged above the microtiter plate for simultaneously adding liquid into multiple assay wells, an illumination system beneath the microtiter plate for illuminating the multiple assay wells simultaneously through the transparent base, a detection system for detecting electromagnetic radiation from the multiple assay wells simultaneously through the transparent base, adding 0.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: April 6, 2021
    Assignee: BAYER PHARMA AKTIENGESELLSCHAFT
    Inventors: Andreas Schade, Mike Küster, Klaus Ochmann, Michael Harnau, Karl-Hermann Koeching, Nils Burkhardt, Bernd Kalthof, Linn Schneider, Georg Schmidt
  • Patent number: 10943729
    Abstract: An entangled inductor structure generates opposite polarity internal magnetic fields therein to substantially reduce, or cancel, external magnetic fields propagating outside of the entangled inductor structure. These reduced external magnetic fields propagating outside of the entangled inductor structure effectively reduce a keep out zone (KOZ) between the entangled inductor structure and other electrical, mechanical, and/or electro-mechanical components. This allows the entangled inductor structure to be situated closer to these other electrical, mechanical, and/or electro-mechanical components within the IC as compared to conventional inductors which generate larger external magnetic fields.
    Type: Grant
    Filed: June 28, 2019
    Date of Patent: March 9, 2021
    Inventors: Ka Fai Chang, Chin-Chou Liu, Fong-Yuan Chang, Hui Yu Lee, Yi-Kan Cheng
  • Patent number: 10934538
    Abstract: A method of creating a miniature multicellular biological construct and a method for studying cellular environments using the miniature multicellular biological construct is provided. The method for making the miniature multicellular biological construct includes suspending cells in a hydrogel, depositing the cell-suspension into a microwell, gelling the cell-suspension, and incubating the cell-suspension. The method for studying cellular environments includes imaging the miniature multicellular biological construct.
    Type: Grant
    Filed: January 12, 2017
    Date of Patent: March 2, 2021
    Assignee: Cleveland State University
    Inventor: Moo-Yeal Lee
  • Patent number: 10844438
    Abstract: A method for detecting genes sensitive to high-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to high-level ionizing radiation discovered in a carcinogenic entity and verified in a normal entity are detected, by subjecting a cancerous AKR/J mouse and a normal ICR mouse to high-level radiation. Thymus is collected therefrom and fatty acid metabolism-related genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, the present invention allows a gene having a specific reaction to radiation to be accurately detected by preventing the interference of confounding variables.
    Type: Grant
    Filed: April 18, 2018
    Date of Patent: November 24, 2020
    Assignee: KOREA HYDRO & NUCLEAR POWER CO., LTD
    Inventors: Hee Sun Kim, Seung Jin Choi, Moo Hyun Choi, Jin Jong Bong, Seok Cheol Shin
  • Patent number: 10823731
    Abstract: Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples. Specifically, methods are provided for detecting and measuring, in a sample, cell morphology; measurement of cell numbers; detection of particles; measurement of particle numbers; and other properties and quantities of or in a sample. Some embodiments may use a sample holder comprising a sample chamber configured to hold said sample, at least a portion of said sample holder comprising an optically transmissive material, said optically transmissive material comprising an optically transmissive surface and a reflective surface.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: November 3, 2020
    Assignee: Labrador Diagnostics LLC
    Inventors: Chinmay Pangarkar, Karan Mohan, James R. Wasson
  • Patent number: 10753903
    Abstract: A technique for electrical detection of a molecule is provided. A fluidic bridge is formed between a nanopipette and a fluid cell, where the molecule is in the nanopipette. A voltage difference is applied between the nanopipette and the fluid cell, where the fluid cell contains an electrolyte solution. Entry of the molecule into the fluidic bridge is determined by detecting a fore pulse. The fluidic bridge between the nanopipette and the fluid cell is broken to form a nanogap. In response to waiting a time interval, the fluidic bridge is reformed between the nanopipette and the fluid cell to close the nanogap. The molecule is determined to exit the nanopipette by detecting an after pulse.
    Type: Grant
    Filed: November 28, 2017
    Date of Patent: August 25, 2020
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Venkat K. Balagurusamy, Stanislav Polonsky
  • Patent number: 10753920
    Abstract: Devices, systems and methods for optical analysis of cells in microgravity are disclosed. Effective cellular microscopic and image analysis requires placement of cells into a proper focal plane, region, or volume. Such placement often requires immobilization of the cells. Cell settling onto a substrate is often sufficient for cell immobilization; however, no significant settling occurs in microgravity. Cell immobilization in microgravity may be accomplished by treatment of a substrate, the cells, or both effective that the substrate captures and immobilizes the cells for inspection. Cells may be immobilized in microgravity by adhering magnetic particles to the cells and applying a magnetic field. Cells may be placed in a proper location for viewing in microgravity by placing the cells into a small chamber or narrow channel. Centrifugal force may effect cell settling and aid cell immobilization. Proper placement or immobilization of cells aids cellular microscopic and image analysis in microgravity.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: August 25, 2020
    Assignee: Labrador Diagnostics LLC
    Inventors: Chinmay Pangarkar, Elizabeth A. Holmes
  • Patent number: 10732614
    Abstract: A process design and management system for batch manufacturing of pharmaceuticals products. The system permits a user to create a chemical process design based on the user's input data and retrieved process library data which includes material data, process data, and equipment data. The system includes software objects defining operations sequences, and processing operation parameters including materials flows and balances, cycle time, constraints, equipment, generic equipment capability requirements, specific equipment capability requirements, and actual capacity analysis. A graphical user interface allowing multiple views of the chemical process design, including one or more of a design view, process flow view, time cycle view, and instructions view.
    Type: Grant
    Filed: September 27, 2018
    Date of Patent: August 4, 2020
    Assignee: Neo PLM, Inc.
    Inventors: Cathal J. Strain, Adam Lalonde, Richard W. Kimball, Majdi Rajab
  • Patent number: 10494593
    Abstract: A culture chamber includes a plurality of recesses (10) each formed of a bottom portion (11) and an opening portion (12). The bottom portion (11) has a hemispherical shape and the opening portion (12) is defined by a wall that surrounds an area from a boundary between the opening portion (12) and the bottom portion (11) to an end of each of the recesses (10), the wall having a taper angle in a range from 1 degree to 20 degrees. An equivalent diameter of the boundary is in a range from 50 ?m to 2 mm and a depth from a bottom of the bottom portion (11) to the end of each of the recesses is in a range from 0.6 or more times to 3 or less times the equivalent diameter, and the wall defining the opening portion (12) forms a surface continuous to the bottom portion (11).
    Type: Grant
    Filed: June 5, 2014
    Date of Patent: December 3, 2019
    Assignees: CORNING INCORPORATED, PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY
    Inventors: Yoko Ejiri, Satoru Ayano, Naoto Fukuhara, Hideki Taniguchi, Takanori Takebe
  • Patent number: 10491838
    Abstract: A portable multispectral imaging and display apparatus includes an imaging device and a display device. The imaging device includes a first image sensor configured to obtain image information of light radiation, came through the lens module, of a first spectral range, a second image sensor configured to obtain image information of light radiation, came through the first image sensor, of the second spectral range, the lens module configured to focus the image information of the light radiation of the first spectral range on the first image sensor, and focus the image information of the light radiation of the second spectral range on the second image sensor. The display device is configured to show the image information of the light radiation of the first spectral range and the image information of the light radiation of the second spectral range.
    Type: Grant
    Filed: January 12, 2018
    Date of Patent: November 26, 2019
    Inventor: Zhongshou Huang
  • Patent number: 10465250
    Abstract: The present invention relates to pancreatic cancer, and more particularly to the survival prognosis of a patient suffering from pancreatic cancer. The invention also aims to determine the suitability of said patient to receive a treatment for pancreatic cancer, in particular a treatment with gemcitabine. The invention also concerns a method for monitoring the effectiveness of a treatment for pancreatic cancer, and advantageously a treatment with gemcitabine, by implementing the method.
    Type: Grant
    Filed: August 13, 2015
    Date of Patent: November 5, 2019
    Assignee: ACOBIOM
    Inventor: David Piquemal
  • Patent number: 10434508
    Abstract: A capsule (100) comprising a housing (110) in which are disposed nanofluidic biosensors (120), a fluid connecting element (140), a filter (150) and a cover (160) is described. The capsule (100) allows the analysis of a fluid sample (300) that would be deposited in the capsule system (100) by a pipette (400). The fluid sample (300) is filtered when passing through a filter (150), then transferred by a fluid connecting element (140) to the inlets of one or several nanofluidic biosensors (120). The capsule system (100) is disposed on an external support (200), and finally an optical or an electrical measurement unit (500) is used to measure the molecular interactions in the nanofluidic biosensors.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: October 8, 2019
    Assignee: Abionic SA
    Inventors: Iwan Maerki, Lauriane Carrere, Nicolas Durand
  • Patent number: 10426143
    Abstract: Systems and methods for object enumeration in a culture tank. The methods comprise: obtaining an array of two dimensional images of an illuminated scene in a culture tank; correcting non-uniform background illumination in the two dimensional images; improving a color contrast between a background and object edges shown in the two dimensional images; performing a defocus analysis using the two dimensional images to generate a focus map image; generating a modified focus map image by eliminating isolated spots shown in the focus map image and merging relatively small spots with adjacent relatively large objects in the focus map image; classifying each object shown in the modified focus map image as an object of interest or an object of no interest; and counting the number of objects classified as objects of interest.
    Type: Grant
    Filed: January 23, 2018
    Date of Patent: October 1, 2019
    Assignee: FLORIDA ATLANTIC UNIVERSITY BOARD OF TRUSTEES
    Inventors: Bing Ouyang, Paul S. Wills, Fraser Dalgleish, Anni Dalgleish
  • Patent number: 10295544
    Abstract: This disclosure provides high throughput immunoblot methods and apparatus for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The method yields highly sensitive and accurate results and is useful in analyze complex system including an antigen from cell or tissue lysate.
    Type: Grant
    Filed: February 15, 2017
    Date of Patent: May 21, 2019
    Assignee: QUANTICISION DIAGNOSTICS INC.
    Inventor: Jiandi Zhang
  • Patent number: 10254268
    Abstract: Disclosed is a method for detecting toxic metal ions in a sample. The method includes: a) preparing a solution of organic acid-bound gold nanoparticles; b) adding a sample containing toxic metal ions to the solution prepared in a) to allow the gold nanoparticles to aggregate; c) dropping the reaction solution obtained in b) onto a silicon substrate and drying the reaction solution such that the gold nanoparticle aggregates are immobilized on the silicon substrate; and d) analyzing the characteristics of the gold nanoparticles immobilized on the silicon substrate. The method enables the detection of even a trace amount of toxic metal ions in a sample with high sensitivity. Therefore, the method can be applied to the management of water quality in food service providers and hospitals, the measurement of contaminants in water supply and drainage systems, and the management of industrial wastewater.
    Type: Grant
    Filed: March 21, 2017
    Date of Patent: April 9, 2019
    Assignee: Korea University Research and Business Foundation, Sejong Campus
    Inventors: Jinsung Park, Woong Kim, Joohyung Park, Seongjae Jo, Minwoo Kim, Gyudo Lee
  • Patent number: 10168346
    Abstract: A method for determining the presence or absence of disposable pipette tips in pipette tip carriers on the work area of a laboratory workstation. Each of the pipette tip carriers has a support panel with receiving holes into each of which a disposable pipette tip can be inserted. The laboratory workstation for carrying out the method has a robot arm with at least one pipette which is designed to receive and dispose of disposable pipette tips. The laboratory workstation has a digital camera which is arranged on a support device and is operatively connected to an analyzing unit. The work area of the laboratory workstation can be completely imaged in at least one first direction using the digital camera.
    Type: Grant
    Filed: April 17, 2015
    Date of Patent: January 1, 2019
    Assignee: TECAN TRADING AG
    Inventors: Martin Schmidt, Rainer Kerkmann
  • Patent number: 10023897
    Abstract: A biosensor chip is placed in a biosensor device and is rotated while a biochemical analysis specimen is measured, the biosensor chip comprising a main body, a holding chamber, a dispensing chamber, a plurality of quantification chambers, and a plurality of measurement chambers. The main body has an inlet into which the specimen is poured. The holding chamber holds the poured specimen inside the main body. The dispensing chamber is connected to the holding chamber via a first channel and dispenses the specimen. The quantification chambers are connected to the dispensing chamber, hold a specific amount of the dispensed specimen, and are disposed at positions located away from a rotational center of a rotary motion according to a distance from the first channel. The measurement chambers are connected to the quantification chambers via a second channel and react the specimen with a biochemical analysis reagent.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: July 17, 2018
    Assignee: PHC HOLDINGS CORPORATION
    Inventors: Kazuyoshi Mori, Masahiro Kouge, Fumihisa Kitawaki, Takako Matsumura, Seiji Onishi
  • Patent number: 9976174
    Abstract: Method includes positioning a first carrier assembly on a system stage. The carrier assembly includes a support frame having an inner frame edge that defines a window of the support frame. The first carrier assembly includes a first substrate that is positioned within the window and surrounded by the inner frame edge. The first substrate has a sample thereon. The method includes detecting optical signals from the sample of the first substrate. The method also includes replacing the first carrier assembly on the system stage with a second carrier assembly on the system stage. The second carrier assembly includes the support frame and an adapter plate held by the support frame. The second carrier assembly has a second substrate held by the adapter plate that has a sample thereon. The method also includes detecting optical signals from the sample of the second substrate.
    Type: Grant
    Filed: March 22, 2016
    Date of Patent: May 22, 2018
    Assignee: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Stephen Rawlings, Venkatesh Mysore Nagaraja Rao, Beng Keong Ang, Nitin Udpa
  • Patent number: 9869658
    Abstract: A technique for electrical detection of a molecule is provided. A fluidic bridge is formed between a nanopipette and a fluid cell, where the molecule is in the nanopipette. A voltage difference is applied between the nanopipette and the fluid cell, where the fluid cell contains an electrolyte solution. Entry of the molecule into the fluidic bridge is determined by detecting a fore pulse. The fluidic bridge between the nanopipette and the fluid cell is broken to form a nanogap. In response to waiting a time interval, the fluidic bridge is reformed between the nanopipette and the fluid cell to close the nanogap. The molecule is determined to exit the nanopipette by detecting an after pulse.
    Type: Grant
    Filed: July 22, 2014
    Date of Patent: January 16, 2018
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Venkat K. Balagurusamy, Stanislav Polonsky
  • Patent number: 9796748
    Abstract: The invention provides systems and methods for spatial sequestration of elements in nucleic acid circuits.
    Type: Grant
    Filed: May 2, 2012
    Date of Patent: October 24, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Thomas E. Schaus, David Yu Zhang, Wei Sun, Peng Yin
  • Patent number: 9643178
    Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyzes. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyzes requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyzes.
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: May 9, 2017
    Assignee: Fluidigm Corporation
    Inventors: Marc Unger, Ian D. Manger, Michael Lucero, Yong Yi, Emily Miyashita-Lin, Anja Wienecke, Geoffrey Facer
  • Patent number: 9513224
    Abstract: Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.
    Type: Grant
    Filed: January 22, 2014
    Date of Patent: December 6, 2016
    Assignee: Theranos, Inc.
    Inventors: Karan Mohan, Chinmay Pangarkar, James Wasson
  • Patent number: 9494521
    Abstract: Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.
    Type: Grant
    Filed: July 25, 2013
    Date of Patent: November 15, 2016
    Assignee: Theranos, Inc.
    Inventors: Elizabeth A. Holmes, Chinmay Pangarkar, Timothy Smith, Karan Mohan, Samartha Anekal, Daniel L. Young, James R. Wasson
  • Patent number: 9428801
    Abstract: The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.
    Type: Grant
    Filed: December 29, 2011
    Date of Patent: August 30, 2016
    Assignee: IBIS BIOSCIENCES, INC.
    Inventors: Mark Eshoo, Stanley Motley, John Picuri
  • Patent number: 9399214
    Abstract: A cartridge includes a first drum having a first chamber and a displacement device that is configured to rotate the first drum about a central axis to connect the first chamber to a second chamber in a conductive manner. The cartridge further includes an electric switch that is configured to be actuated by the displacement device between a closed switching state and an open switching state.
    Type: Grant
    Filed: June 7, 2012
    Date of Patent: July 26, 2016
    Assignee: Robert Bosch GmbH
    Inventors: Martina Daub, Juergen Steigert
  • Patent number: 9376713
    Abstract: Provided are methods and devices for label-free detection of nucleic acids that are amplified by polymerase chain reaction. A solution containing the components necessary for a PCR is introduced to a microfluidic amplification chamber and an electric field applied to a confined region in which PCR occurs. PCR product generated in the confined region is detected by measuring an electrical parameter that is, for example, solution impedance. The devices and methods provided herein are used, for example, in assays to detect one or more pathogens or for point-of-care tests. In an aspect, the PCR product is confined to droplets and the assay relates to detecting an electrical parameter of a flowing droplet, thereby detecting PCR product without a label. In an aspect, the PCR occurs in the droplet.
    Type: Grant
    Filed: September 23, 2010
    Date of Patent: June 28, 2016
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Rashid Bashir, Yi-Shao Liu, Eric Salm, Woo-Jin Chang, Nicholas N. Watkins
  • Patent number: 9290798
    Abstract: The invention provides methods to process multiple microarrays by providing a microarray plate and processing plates. In an embodiment of the invention, methods for assembling microarray plates by using wafers are described for high throughput microarray processing.
    Type: Grant
    Filed: July 2, 2013
    Date of Patent: March 22, 2016
    Assignee: Affymetrix, Inc.
    Inventor: Mohsen Shirazi
  • Patent number: 9116139
    Abstract: A technique is disclosed for sample management for use in conjunction with sequencing devices that sequence biological samples, e.g., DNA and RNA. A sequencing device or a network of sequencing devices may be scheduled according to the characteristics of the samples in queue and the capabilities and availability of sequencing devices. Biological samples may be automatically queued and loaded via a sample distribution system. A sample distribution system may be used to reduce operator intervention.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: August 25, 2015
    Assignee: ILLUMINA, INC.
    Inventors: Robert C. Kain, Alexander G. Dickinson, Min-Jui Richard Shen, Helmy A. Eltoukhy, Francisco Jose Garcia
  • Patent number: 9064965
    Abstract: This application discloses ZnO film transistor-based immunosensors (ZnO-bioTFT), 2T biosensor arrays formed from two integrated ZnO-bioTFTs, 1T1R-based nonvolatile memory (NVM) arrays formed from ZnO-bioTFT (T) integrated with ZnO-based resistive switches (R), as well as integrated bioTFT (IBTFT) sensor systems formed from 2T biosensor arrays and 1T1R NVM arrays. Through biofunctionalization, these biosensors can perform immunosensing with high sensitivity and selectivity, and therefore have a wide range of applications, for example, in detecting target biomolecules or small molecules, and potential application in cancer diagnosis and treatment.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: June 23, 2015
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: Yicheng Lu, Pavel Ivanoff Reyes, Ki-Bum Lee, Aniruddh Solanki, Chieh-Jen Ku
  • Publication number: 20150148265
    Abstract: Devices and systems for sampling and profiling microbiota of skin are described which include a replaceable microbe sampling unit including a substrate and a location information storage component, the substrate including a microbe-capture region configured to capture at least one type of microbe from one or more regions of a skin surface of an individual, and the location information storage component configured to store information associated with the location of said one or more regions of the skin surface of the individual.
    Type: Application
    Filed: November 27, 2013
    Publication date: May 28, 2015
    Inventors: Michael H. Baym, Roderick A. Hyde, Jordin T. Kare, Eric C. Leuthardt, Gary L. McKnight, Tony S. Pan, Elizabeth A. Sweeney, Clarence T. Tegreene, Lowell L. Wood, JR.
  • Publication number: 20150148258
    Abstract: The present invention describes a method and devices for the simultaneous detection, recovery identification and counting of a plurality of microorganisms consisting in providing mixtures of nutrients specially selected from those that curtail the lag phase of growth in bacteria and moulds and which, together with fluorescent enzymatic, chromogenic or bioluminescent markers and other nutrient components or growth inhibitors, are embedded in three-dimensional structures or natural or artificial clays or ceramics with cavities of different dimensions and forms and specific surface areas of between 2×103 and 6×108 m2/m3.
    Type: Application
    Filed: March 27, 2013
    Publication date: May 28, 2015
    Inventors: Claudio Rodriguez Martinez, Jesus Eduardo Gonzalez Ruiz, Tamara Lobaina Rodriguez, Raisa Zhurbenko, Ana Iris Brito Gonzales, Monica Lopez Hernandez, Javier Fernandez Aragon, Ivonne Alfonso Valdes, Adelaida Ortea Suris
  • Publication number: 20150148242
    Abstract: The present application is directed to methods and systems for identifying small molecule compounds in mixtures using a library comprising calculated structures and corresponding calculated mass spectral fragmentation patterns of known and/or hypothetical small molecule compounds that may be in the mixture and screening of a mass spectrum of the mixture using the library to identify matching fragmentation patterns. If a mass spectral fragmentation pattern present in the mass spectrum of the mixture matches a calculated fragmentation pattern of one of the known or hypothetical compounds this confirms the identity of a compound in the mixture as the known or hypothetical compound. The method represents a platform method that can be used for a multitude of purposes related to the screening and identification of compounds in mixtures.
    Type: Application
    Filed: June 5, 2013
    Publication date: May 28, 2015
    Applicant: McMaster University
    Inventors: Nathan Magarvey, Aubrey Bailey Morgan Wyatt, Chad William Johnston, Ashraf Ibrahim, Bin Ma, Lian Yang
  • Patent number: 9040463
    Abstract: The invention provides a multiplexed assay device comprising a reaction chamber and several sets of encoded microcarriers 2 wherein the reaction chamber is a microchannel 1 and wherein the longitudinal movement of the microcarriers 2 is restricted and wherein the microcarriers 2 have a shape in relation to the geometry of the microchannel 1 such that at least two can stand side by side in the microchannel 1 without touching each other and without touching the perimeter of the microchannel 1 and are preferably observable in the reaction chamber. Moreover, the invention provides a method for performing multiplexed assay based on microcarriers 2 that improves mass transfer, simplifies the preparation and the execution of the assay and facilitates readout of biological reactions and identity of microcarriers 2.
    Type: Grant
    Filed: December 23, 2009
    Date of Patent: May 26, 2015
    Assignee: MYCARTIS NV
    Inventors: Nicolas Demierre, Nader Donzel, Jose Gil, Philippe Renaud
  • Publication number: 20150141281
    Abstract: The present invention relates to a method for performing a chemical and/or a biological assay comprising the following successive steps of: a) providing an assay device with a microchannel having an inlet and an outlet and further comprising restricting means designed to restrict the movement toward the outlet of microparticles introduced in the microchannel while letting a fluid to flow through the restricting means, b) introducing microparticles in the microchannel via the inlet, c) restricting the movement of said microparticles in the microchannel toward the outlet using restricting means, d) flowing a fluid sample through the microchannel, e) performing a biological and/or chemical read-out on each microparticle, the method further comprising the steps of: f) moving the microparticles in the microchannel, and g) repeating successively the steps d) and e).
    Type: Application
    Filed: July 10, 2013
    Publication date: May 21, 2015
    Applicant: MYCARTIS NV
    Inventors: Raphael Tornay, Nicolas Demierre, Jose De Gil, Robin Muller, Didier Falconnet
  • Publication number: 20150141264
    Abstract: The invention provides a method for in-field detection of a distinctive marker. The method includes providing a sample from an article of interest and analyzing the sample to detect the presence of the distinctive marker. The analysis is performed using an in-field detection instrument. The in-field detection instrument includes a microsystem configured to perform sample in-answer out analysis and detect the presence of the distinctive marker in the sample.
    Type: Application
    Filed: August 28, 2014
    Publication date: May 21, 2015
    Inventors: Lawrence Jung, MingHwa Benjamin Liang
  • Publication number: 20150133321
    Abstract: The present disclosure relates to characterization of biological samples. By way of example, a biological sample may be contacted with a plurality of probes specific for targets in the sample, such as probes for immune markers and segmenting probes. Acquired image data of the sample may be used to segment the images into epithelial and stromal regions to characterize individual cells in the sample based on the binding of the probes. Further, the biological sample may be characterized by a distribution, location, and type of a plurality of the characterized cells.
    Type: Application
    Filed: November 13, 2013
    Publication date: May 14, 2015
    Applicant: General Electric Company
    Inventors: Srabani Bhaumik, Michael John Gerdes, Zhengyu Pang, Fiona Ginty, Christopher James Sevinsky, JR., Qing Li, Alberto Santamaria-Pang, Yunxia Sui, Keyur Hemantkumar Desai
  • Publication number: 20150133345
    Abstract: The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.
    Type: Application
    Filed: November 10, 2014
    Publication date: May 14, 2015
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra
  • Publication number: 20150126404
    Abstract: A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.
    Type: Application
    Filed: November 5, 2014
    Publication date: May 7, 2015
    Inventors: Yanqing Tian, Liqiang Zhang, Fengyu Su, Deirdre Meldrum, Sean Buizer, Clifford L. Anderson, Laimonas Kelbauskas, Kristen Lee
  • Publication number: 20150126409
    Abstract: The present invention relates to the detection of microbial organisms using non-toxic nanostructured sensors that change their physical or chemical properties upon detecting these microbial organisms. These sensors allow an unskilled person to rapidly detect the presence of microbial contamination.
    Type: Application
    Filed: November 3, 2014
    Publication date: May 7, 2015
    Inventors: Khashayar Ghandi, Zahid Mahimwalla, Tan Yang, Cody Landry
  • Publication number: 20150119280
    Abstract: Techniques for hydrogel microstructures with oil isolation for small reaction volumes include providing a hydrogel microstructure that has a plurality of pores and a hydrogel frame surrounding the pores. The microstructure has a volume in a range from about 1 picoliter to about 10,000 picoliters and is configured to repel an immiscible fluid. Each pore of the plurality of pores has a pore size configured to pass the target molecule in a first solution. The microstructure is contacted with the first solution, and with a second solution that includes a reactant molecule that reacts with the target molecule to produce an observable product molecule. The microstructure is encompassed with the immiscible fluid for an extended observation duration from about 1 to about 10,000 seconds, wherein the immiscible fluid does not pass into the pores but traps the product in the microstructure. The observable product molecule is measured at some time during the observation duration.
    Type: Application
    Filed: October 27, 2014
    Publication date: April 30, 2015
    Applicant: Massachusetts Institute of Technology
    Inventors: Rathi Lakshmi Srinivas, Patrick S. Doyle
  • Publication number: 20150119268
    Abstract: A biological sample processing apparatus having an enclosure. A plurality of sample processing modules are held by the enclosure. Each sample processing module is configured to hold a removable sample cartridge and to only perform sample processing on a sample within the corresponding removable sample cartridge. Each sample processing module is configured to perform at least one of a plurality of testing processes on the sample within the removable sample cartridge. At least one module in the apparatus is configured to perform nucleic acid amplification and detection.
    Type: Application
    Filed: April 25, 2013
    Publication date: April 30, 2015
    Applicant: CEPHEID
    Inventor: John L. Bishop
  • Publication number: 20150119298
    Abstract: A system for performing one or more microfluidic processes includes an integrated fluidic device comprising a plurality of well regions and a plurality of control valves and a workflow manager. The system also includes a transfer robot adapted to transfer the integrated fluidic device between a plurality of stations in response to a series of instructions from the workflow manager and a first station comprising a dispensing robot adapted to dispense at least one of a plurality of sample solutions and at least one of a plurality of reagents into the integrated fluidic device. The system further includes a second station comprising a fluidic controller unit and a third station comprising an inspection station.
    Type: Application
    Filed: September 29, 2014
    Publication date: April 30, 2015
    Inventors: Gang Sun, Greg Harris, Andy May, Kyle Self, Kevin Farrell, Paul Wyatt
  • Publication number: 20150119274
    Abstract: The present invention describes microplates with arrays of wells containing magnetic inserts in the form of disks, cylinders or otherwise shaped materials with apertures in the centre for optical interrogation of the contacting solution. These inserts are capable of capturing ferro- and paramagnetic nano- and microparticles. The subject microplates find use in a variety of applications, including clinical and environmental assays, high throughput screening for genomics, proteomics and pharmaceutical applications, point-of-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally and high-throughput screening of materials for separation and catalysis.
    Type: Application
    Filed: June 3, 2013
    Publication date: April 30, 2015
    Inventors: Sergey Anatoliyovich Piletsky, Olena Piletska, Antonio Guerreiro, Alessandro Poma, Khalku Karim, Stanislav Piletsky
  • Publication number: 20150119297
    Abstract: There are systems and methods for performing an assay to generate genotype information about a subject associated with a medical condition. There are also systems and method for generating and utilizing prognostic information associated with treating the patient with a medication based on the genotype information and an association of the genotype and metabolizing the medication. The genotype information includes data relating to SNP alleles in the patient's genotype and the association of the alleles and metabolism of a medication by the patient.
    Type: Application
    Filed: October 28, 2013
    Publication date: April 30, 2015
    Inventor: Brian Meshkin
  • Publication number: 20150119296
    Abstract: Novel and improved systems and methods for high speed arraying, hybridization, quantitative development and/or assaying are provided. Some embodiments provide a web based arraying format. Some other embodiments provide a sheet based arraying format. Some embodiments use a drop on drop assaying or hybridization mode. In some embodiments, a substantially inert substrate is utilized. In some other embodiments, an interactive substrate is utilized.
    Type: Application
    Filed: December 23, 2014
    Publication date: April 30, 2015
    Inventors: Thomas C. Tisone, Holger Eickhoff
  • Publication number: 20150119286
    Abstract: A high-throughput corrosion testing mechanism was developed for metals in a variety of environments in controlled, multiplexed microenvironments. Many parallel experiments can be conducted with microbial and environmental variables independently manipulated to identify the key determinants of corrosion progression. The synthetic assay design enables subsequent surface characterization of select samples within the array. In as little as one day, diverse corrosive environments can be compared quantitatively.
    Type: Application
    Filed: October 29, 2014
    Publication date: April 30, 2015
    Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Matthew James Whitfield, Krystyn J. Van Viet
  • Publication number: 20150119262
    Abstract: This disclosure relates generally to the prediction of a gene target of an enhancer based on genomic proximity and a comparison to a set of known enhancer-gene pairs. A gene can be selected a gene from a given cell type. A domain can be established bi-directionally around the gene, and enhancers located within the domain can be identified. The enhancers located within the domain can be considered to be in close genomic proximity to the gene. For each enhancer, a test pair of the enhancer and the gene can be established and compared to a set of known enhancer-gene pairs. The prediction can be established based on the comparison and the genomic proximity.
    Type: Application
    Filed: October 27, 2014
    Publication date: April 30, 2015
    Inventor: Peter C/ Scacheri