For Creating A Library Patents (Class 506/40)
  • Patent number: 9267170
    Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: February 23, 2016
    Assignee: Life Technologies Corporation
    Inventors: Ronald Danehy, Gary Lim, Jacob Freudenthal
  • Patent number: 9157161
    Abstract: A thin film with multiple binding functionality can be prepared on an electrode surface via consecutive electroreduction of two or more aryl-onium salts with different functional groups. This versatile and simple method for forming multifunctional surfaces provides an effective means for immobilization of diverse molecules at close proximities. The multifunctional thin film has applications in bioelectronics, molecular electronics, clinical diagnostics, and chemical and biological sensing.
    Type: Grant
    Filed: July 14, 2011
    Date of Patent: October 13, 2015
    Assignee: Sandia Corporation
    Inventors: Susan M. Brozik, Jason C. Harper, Ronen Polsky, David R. Wheeler, Dulce C. Arango, Shawn M. Dirk
  • Patent number: 9085461
    Abstract: A wafer having a plurality of dies (also called array chips) on the wafer, the die having an electrode to generate a deprotecting reagent, a working electrode to electrochemically synthesize a material, a confinement electrode adjacent to the working electrode to confine reactive reagents, and a die pad, wherein die pads of the plurality of dies are interconnected on the wafer to electrochemically synthesize the material in parallel on a plurality of working electrodes is disclosed. Also, a method for wafer-scale manufacturing of a plurality of dies and a method for electrochemically synthesizing a material in parallel on a plurality of dies on a wafer are disclosed.
    Type: Grant
    Filed: May 16, 2012
    Date of Patent: July 21, 2015
    Assignee: INTEL CORPORATION
    Inventors: Valery M. Dubin, Florian Gstrein, Gordon D. Holt, Brandon Barnett
  • Publication number: 20150148258
    Abstract: The present invention describes a method and devices for the simultaneous detection, recovery identification and counting of a plurality of microorganisms consisting in providing mixtures of nutrients specially selected from those that curtail the lag phase of growth in bacteria and moulds and which, together with fluorescent enzymatic, chromogenic or bioluminescent markers and other nutrient components or growth inhibitors, are embedded in three-dimensional structures or natural or artificial clays or ceramics with cavities of different dimensions and forms and specific surface areas of between 2×103 and 6×108 m2/m3.
    Type: Application
    Filed: March 27, 2013
    Publication date: May 28, 2015
    Inventors: Claudio Rodriguez Martinez, Jesus Eduardo Gonzalez Ruiz, Tamara Lobaina Rodriguez, Raisa Zhurbenko, Ana Iris Brito Gonzales, Monica Lopez Hernandez, Javier Fernandez Aragon, Ivonne Alfonso Valdes, Adelaida Ortea Suris
  • Publication number: 20150148238
    Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 28, 2015
    Applicants: ADVANCED LIQUID LOGIC, INC., Duke University
    Inventors: Vamsee Pamula, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair
  • Publication number: 20150141261
    Abstract: The present disclosure generally relates to sequencing two or more genes expressed in a single cell in a high-throughput manner. More particularly, the present disclosure relates to a method for high-throughput sequencing of pairs of transcripts co expressed in single cells (e.g., antibody VH and VL coding sequence) to determine pairs of polypeptide chains that comprise immune receptors.
    Type: Application
    Filed: June 17, 2013
    Publication date: May 21, 2015
    Inventors: Scott Hunicke-Smith, Brandon Dekosky, Andy Ellington, George Georgiou
  • Publication number: 20150126412
    Abstract: A method and an array filling system for loading a plurality of disparate sample containers, the sample containers comprising an integral structure. Each receptacle is characterized by a hydrophilic surface, and the receptacles are separated by a hydrophobic surface. The system has a liquid transfer device capable of holding liquid and adapted for motion to cause sequential communication of liquid held in the liquid transfer device with successive receptacles of the array by dragging the liquid across the hydrophobic surface.
    Type: Application
    Filed: June 10, 2014
    Publication date: May 7, 2015
    Inventors: Ian Hunter, Colin J.H. Brenan
  • Publication number: 20150126411
    Abstract: The systems and methods described herein relate to a high-throughput flow apparatus. The apparatus is used with an array of wells, and is configured to impart a predetermined shear stress on cells cultured within each of the wells of the array of wells. The apparatus includes a plurality of mechanical tips. The plurality of mechanical tips each includes a head with a hemispheroid shape. The apparatus also includes a motor associated with at least one of plurality of mechanical tips. The motor is configured to drive the plurality of mechanical tips to impart the shear stress pattern in each of the wells.
    Type: Application
    Filed: November 6, 2014
    Publication date: May 7, 2015
    Inventors: Guillermo Garcia-Cardena, Peter Mack, Jeffrey T. Borenstein, Ahmad S. Khalil, Eli J. Weinberg, Jason O. Fiering, Ernest S. Kim, William J. Adams, JR., Mitchell Hansberry, Stephen Bellio
  • Publication number: 20150119274
    Abstract: The present invention describes microplates with arrays of wells containing magnetic inserts in the form of disks, cylinders or otherwise shaped materials with apertures in the centre for optical interrogation of the contacting solution. These inserts are capable of capturing ferro- and paramagnetic nano- and microparticles. The subject microplates find use in a variety of applications, including clinical and environmental assays, high throughput screening for genomics, proteomics and pharmaceutical applications, point-of-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally and high-throughput screening of materials for separation and catalysis.
    Type: Application
    Filed: June 3, 2013
    Publication date: April 30, 2015
    Inventors: Sergey Anatoliyovich Piletsky, Olena Piletska, Antonio Guerreiro, Alessandro Poma, Khalku Karim, Stanislav Piletsky
  • Publication number: 20150111773
    Abstract: The present invention provides methods for rapidly screening and measuring the ligand binding affinity of in vitro selected peptides to the cognate and off-target proteins. This general strategy is amenable to high throughput analysis because the peptides are synthesized by cell-free translation, as opposed to solid-phase synthesis required by traditional assays, and affinities can be readily measured in standard formats.
    Type: Application
    Filed: June 7, 2013
    Publication date: April 23, 2015
    Inventors: John Chaput, Andrew Larsen, Annabelle Gillig
  • Patent number: 8999266
    Abstract: A slotted pin tool, a delivery system containing the pin tool, a substrate for use in the system and methods using the pin tool and system are provided. The slotted pin tool contains a plurality of pins having slotted ends designed to fit around each loci of material deposited on a surface, such as a microarray, without contacting any of the deposited material. Sample is delivered by contacting the pin tool with the surface; the amount delivered is proportional to the velocity of the pin tool as it contacts the surface or the velocity of the liquid when movement of the pin is halted.
    Type: Grant
    Filed: November 21, 2012
    Date of Patent: April 7, 2015
    Assignee: Agena BioScience, Inc.
    Inventors: Xian-Wei Yao, Chao Lin, Paul Heaney, Thomas Becker, Aaron A. Hanson, Michael C. Willis
  • Patent number: 8999726
    Abstract: Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter may be used to deposit highly concentrated spots of protein or other materials on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells, microfluidic conduits, and orifices to deposit proteins, other biomolecules, or chemicals on a spot on, a separate surface. Each orifice is connected to two wells via microconduits. When the spotter contacts a surface, a seal is formed between the orifices and the surface. The same or different substances may be flowed across each orifice. Any number of orifices may be incorporated into a spotter.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: April 7, 2015
    Assignee: University of Utah Research Foundation
    Inventors: David A. Chang-Yen, Sriram Natarajan, Josh Eckman, Bruce K. Gale, David Myszka
  • Publication number: 20150094238
    Abstract: A system for inserting reagent or macrobeads into bores of a sample plate is disclosed comprising an insertion device arranged to retain one or more reagent or macrobeads by means of a vacuum whilst inserting the reagent or macrobeads into bores of the sample plate.
    Type: Application
    Filed: March 6, 2013
    Publication date: April 2, 2015
    Applicant: Dynex Technologies, Inc.
    Inventors: Adrian Bunce, Andrew Fusellier
  • Publication number: 20150072897
    Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85+/?15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.
    Type: Application
    Filed: March 15, 2013
    Publication date: March 12, 2015
    Inventors: Michael C. Pallas, James C. Nurse, Gary Lim, Theodore E. Straub, Evan W. Foster
  • Publication number: 20150072900
    Abstract: Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.
    Type: Application
    Filed: September 26, 2014
    Publication date: March 12, 2015
    Applicants: DUKE UNIVERSITY, ADVANCED LIQUID LOGIC, INC.
    Inventors: Vijay Srinivasan, Vamsee K. Pamula, Michael G. Pollack
  • Publication number: 20150057184
    Abstract: Provided is a microarray platform for the culture of cells atop combinatorial matrix mixtures; enabling the study of differentiation in response to a multitude of microenvironments in parallel.
    Type: Application
    Filed: March 25, 2014
    Publication date: February 26, 2015
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Sangeeta N. BHATIA, Christopher FLAIM
  • Patent number: 8961899
    Abstract: The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.
    Type: Grant
    Filed: June 24, 2013
    Date of Patent: February 24, 2015
    Assignee: IBIS Biosciences, Inc.
    Inventors: Mark Eshoo, John Picuri
  • Publication number: 20150051118
    Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 19, 2015
    Inventors: Eliodor Ghenciu, Theodore E. Straub, Evan W. Foster, Michael C. Pallas
  • Publication number: 20150038379
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Application
    Filed: October 10, 2014
    Publication date: February 5, 2015
    Inventors: Emil P. KARTALOV, Darryl SHIBATA, Clive TAYLOR, Lawrence A. WADE
  • Publication number: 20150038377
    Abstract: A liquid dispenser for a microfluidic assay system is described. The dispenser includes at least one transfer pin for transferring a microfluidic sample of liquid to a target receptacle. A pin tip at one end of the transfer pin is structured to cooperate with an opening in the target receptacle. The tip uses a high voltage potential to transfer the sample from the pin to the receptacle.
    Type: Application
    Filed: July 15, 2014
    Publication date: February 5, 2015
    Inventors: Leila Madrone, John Linton, Colin John Herbert Brenan
  • Publication number: 20150018248
    Abstract: Methods and systems for processing a first solution on a respective primary area of a device with a plurality of primary areas and a plurality of secondary areas are described. A method includes providing the device and dispensing a second solution to a respective secondary area of one or more secondary areas adjacent to the respective primary area, thereby mixing the first solution on the respective secondary area and the second solution on the respective primary area. The method also includes removing at least a portion of the mixed solution.
    Type: Application
    Filed: July 9, 2014
    Publication date: January 15, 2015
    Inventor: Namyong KIM
  • Patent number: 8927465
    Abstract: A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray.
    Type: Grant
    Filed: August 3, 2007
    Date of Patent: January 6, 2015
    Assignee: National University University of Singapore
    Inventors: Dieter Trau, Wen-Tso Liu, Kian Kok Johnson Ng
  • Patent number: 8920752
    Abstract: Novel and improved systems and methods for high speed arraying, hybridization, quantitative development and/or assaying are provided. Some embodiments provide a web based arraying format. Some other embodiments provide a sheet based arraying format. Some embodiments use a drop on drop assaying or hybridization mode. In some embodiments, a substantially inert substrate is utilized. In some other embodiments, an interactive substrate is utilized.
    Type: Grant
    Filed: February 1, 2013
    Date of Patent: December 30, 2014
    Assignee: Biodot, Inc.
    Inventors: Thomas C Tisone, Holger Eickhoff
  • Publication number: 20140378348
    Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In some embodiments, the system may include a well and a channel component attached to the well. The channel component may include (a) a body, (b) an input tube (a “fluid pickup”) projecting from a bottom surface of the body and having an open bottom end disposed in the input well, (c) a microchannel, and (d) a passage extending through the input tube and the body and connecting the well to the microchannel. The system may be configured to receive a sample-containing fluid in the well and retain the sample-containing fluid below a top end of the passage, until a pressure differential is created that drives at least a portion of the sample-containing fluid from the well via the passage and through the microchannel.
    Type: Application
    Filed: June 23, 2014
    Publication date: December 25, 2014
    Inventors: Anthony J. Makarewicz, JR., Luc Bousse, Stefano Schiaffino
  • Publication number: 20140371107
    Abstract: Methods, devices and systems for handling sample liquids, encapsulating liquids and magnetic particles are disclosed.
    Type: Application
    Filed: January 25, 2013
    Publication date: December 18, 2014
    Applicant: GENCELL BIOSYSTEMS LTD.
    Inventors: Kieran Curran, David McGuire
  • Publication number: 20140336084
    Abstract: A substrate, which is useful for performing biological, chemical and diagnostic assays, and a method for preparing the substrate are provided. The substrate has an upper surface with a coating disposed thereon. The coating comprises a charged polymer, a non-ionic polyether, and a silicate compound. The substrate can increase capture phase binding and reduce non-specific binding of biomolecules for a biological microarray.
    Type: Application
    Filed: December 20, 2012
    Publication date: November 13, 2014
    Applicant: SIEMENS HEALTHCARE DIAGNOSTICS INC.
    Inventors: Michael J. Pugia, Pratap Singh
  • Publication number: 20140274808
    Abstract: The present invention provides a system for receiving biological sequence information and activating the synthesis of a biological entity. The system has a receiving unit for receiving a signal encoding biological sequence information transmitted from a transmitting unit. The transmitting unit can be present at a remote location from the receiving unit. The system also has an assembly unit connected to the receiving unit, and the assembly unit assembles the biological entity according to the biological sequence information. Thus, according to the present invention biological sequence information can be digitally transmitted to a remote location and the information converted into a biological entity, for example a protein useful as a vaccine, immediately upon being received by the receiving unit and without further human intervention after preparing the system for receipt of the information.
    Type: Application
    Filed: August 16, 2013
    Publication date: September 18, 2014
    Applicant: Synthetic Genomics, Inc.
    Inventors: J. Craig Venter, Daniel Gibson, John E. Gill
  • Publication number: 20140274809
    Abstract: A multi-well manifold assembly and method for reducing cross-contamination in continuous synthesis reactions in channels of microfluidic devices, for example oligonucleotide synthesis.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Inventors: Jon Harvey, Ryan Witt, William E. Martin, III
  • Publication number: 20140249057
    Abstract: The present teachings provide a fluid processing device adapted to produce different oligomers in a plurality of respective reaction sites. The fluid processing device can comprise a first manifold for delivering reactants to the plurality of reaction sites, and a second manifold for removing waste from, and optionally delivering wash fluid to, the plurality of reaction sites. Surface tension control valves can be disposed in fluid communication with the first manifold and can selectively allow reactants and/or fluids into the reaction sites. A method of making oligonucleotides is also provided.
    Type: Application
    Filed: January 31, 2014
    Publication date: September 4, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventor: Sergey V. Ermakov
  • Publication number: 20140235485
    Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.
    Type: Application
    Filed: February 18, 2014
    Publication date: August 21, 2014
    Applicant: INTELLIGENT BIOSYSTEMS INC.
    Inventors: Steven Gordon, Daniel Stetson
  • Publication number: 20140200164
    Abstract: System, including methods and apparatus, for detection of spaced droplets.
    Type: Application
    Filed: January 20, 2014
    Publication date: July 17, 2014
    Inventors: Anthony J. Makarewicz, JR., Amy L. Hiddessen, Kevin D. Ness, Donald. A. Masquelier, Billy W. Colston, JR., Benjamin J. Hindson, Serge Saxonov, Svilen S. Tzonev, Michael Y. Lucero, Ryan T. Koehler
  • Patent number: 8776717
    Abstract: The present invention provides methods and systems for discretized, combinatorial processing of regions of a substrate such as for the discovery, implementation, optimization, and qualification of new materials, processes, and process sequence integration schemes used in integrated circuit fabrication. A substrate having an array of differentially processed regions thereon is processed by delivering materials to or modifying regions of the substrate.
    Type: Grant
    Filed: February 10, 2006
    Date of Patent: July 15, 2014
    Assignee: Intermolecular, Inc.
    Inventors: Tony P. Chiang, David E. Lazovsky, Thomas R. Boussie, Thomas H. McWaid, Alexander Gorer
  • Patent number: 8765078
    Abstract: There is provided a biochip stamping device. The biochip stamping device includes a stamping jig in which a first biochip is aligned; an inverting mechanism vertically inverting a second biochip; and a movement mechanism transferring the vertically inverted second biochip on the stamping jig to combine the first biochip and the second biochip.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: July 1, 2014
    Assignee: Samsung Electro-Mechanics Co., Ltd.
    Inventors: Sang Youl Jeon, Bo Sung Ku, Hee Ju Son
  • Publication number: 20140179565
    Abstract: A system is provided that can include: a plurality of retainment regions, where each retainment region is adapted to retain a respective type of an oligonucleotide supported on a respective support; a mixture retainment region; a handling device; a control unit adapted to control the handling device; and a separating unit adapted to simultaneously separate different supported oligonucleotides from their respective supports. A method is provided that can include: pooling together a plurality of supported oligonucleotides to form a mixture; and simultaneously separating the oligonucleotides of the supported oligonucleotides in the mixture from their supports.
    Type: Application
    Filed: November 25, 2013
    Publication date: June 26, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventor: Charles Vann
  • Patent number: 8759259
    Abstract: Embodiments of the invention are directed to a one-bead-two-compound method for the creation of encoded cyclic peptoid libraries. This scheme is useful for the creation of cyclic peptoid microarrays since only the cyclic peptoid, not the linear encoding molecule, contains an attachment residue and thus can be spotted onto an activated substrate.
    Type: Grant
    Filed: October 15, 2010
    Date of Patent: June 24, 2014
    Assignee: The Board of Regents of the University of Texas System
    Inventors: Yong-Uk Kwon, Thomas Kodadek
  • Publication number: 20140171342
    Abstract: The present disclosure provides apparatuses, systems, and methods involving a spotter for depositing a substance on a submerged surface. The spotter comprises an outlet cavity defined at least in part by a spotting orifice, a first opening, and a second opening. The spotter also comprises a first conduit fluidly coupled to the first opening and a second conduit fluidly coupled to the second opening. The spotter is adapted so that fluid flowing through the first conduit and the second conduit is communicated among the first opening, the second opening, and a submerged deposition surface when the sealing orifice is sealed against the submerged deposition surface to form a deposition spot on the submerged deposition surface. The submerged deposition surface is within a liquid such that the liquid covers the deposition spot upon removal of the orifice from the deposition surface.
    Type: Application
    Filed: September 13, 2013
    Publication date: June 19, 2014
    Inventors: Bruce K. Gale, Joshua W. Eckman, Adam Miles, Christopher Morrow, James Smith, Sriram Natarajan, Mark Eddings
  • Publication number: 20140148347
    Abstract: The present invention provides methods and systems for analyzing mammalian transcriptomes, particularly, for low abundant transcripts, and with the use of high throughput technologies. Heptamer primers and sequence tags generated by the iterative randomized algorithm, as well as the sequencing-library generation system for amplifying and synthesis-based sequencing low abundant transcripts using the heptamer primers are also provided. The present invention further provides the use of the invention system and method for identifying key embryological lineage specific transcripts that anticipate differentiation of specific cell types.
    Type: Application
    Filed: December 13, 2013
    Publication date: May 29, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Vipul Bhargava, Pang Ko, Shankar Subramaniam
  • Publication number: 20140142000
    Abstract: The present invention relates to a microfluidic array platform comprising a substrate and two layers between which one membrane is sandwiched, wherein a plurality of cell culture wells are constructed in the top layer and one or more microfluidic channels for oxygen scavenging reactions or/and oxygen generating reactions to control the oxygen tensions are constructed in the bottom player. The microfluidic array platform is capable of simultaneously performing cell culture under different oxygen tensions and compatible with existing cell incubators and high-throughput instruments for cost-effective setup and straightforward operation.
    Type: Application
    Filed: October 21, 2013
    Publication date: May 22, 2014
    Applicant: ACADEMIA SINICA
    Inventors: Yi-Chung Tung, Chien-Chung Peng, Hsiu-Chen Shih, Wei-Hao Liao
  • Patent number: 8715930
    Abstract: Disclosed are compositions, devices, and methods for loading molecules of interest onto a substrate by contacting beads having molecules of interest attached to them with the substrate, for example by providing a field that brings the beads into proximity or contact with the substrate and moves the beads with respect to the substrate. Such molecules of interest can be deposited onto substrates for single-molecule analysis.
    Type: Grant
    Filed: March 23, 2012
    Date of Patent: May 6, 2014
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Thang Pham, Jeremy Gray, Adrian Fehr, Albert James Chmura, Yu-Chih Tsai, Arunashree Bhamidipati
  • Publication number: 20140121132
    Abstract: The present invention relates to a system for producing a nucleic acid molecule imaging array for use in high resolution imaging of individual nucleic acid molecules. The system includes a micro/nanostructured capture array having a hydrophobic surface having topographical features effective to assist in capillary-based trapping and elongation of individual nucleic acid molecules. The system also includes a transfer platform having a support and a hydrophobic substrate layered on the support. The transfer platform is effective to receive and capture, through solvent mediation, the trapped and elongated individual nucleic acid molecules from the micro/nanostructured capture array. The present invention also relates to a nucleic acid molecule imaging array, a transfer platform for use in preparing a nucleic acid molecule array, and a kit for producing a nucleic acid molecule imaging array for use in high resolution imaging of individual nucleic acid molecules.
    Type: Application
    Filed: March 12, 2012
    Publication date: May 1, 2014
    Applicant: CORNELL UNIVERSITY
    Inventors: Aline Cerf, Harold G. Craighead, Thomas Alava
  • Publication number: 20140066333
    Abstract: The invention provides a method of printing, onto a substrate (12), an array (14) of spots of reagent compositions for use in a chemical and/or biochemical analysis. The method includes displacing an array of reagent composition containing capillary tubes (22) arranged alongside one another from an inoperative position to an operative position in which open ends of the capillary tubes (22) simultaneously impinge against a substrate and thereafter displacing the array of tubes (22) from the operative position back to the inoperative position. The invention extends to a printing apparatus (10), a method of printing a layered array of spots of reagent compositions, a method of introducing reagent compositions into the tubes, a reagent introducing device for introducing reagent compositions into the tubes and a printing installation which includes the printing apparatus (10) and the reagent introducing device.
    Type: Application
    Filed: November 8, 2013
    Publication date: March 6, 2014
    Inventor: Neil Emans
  • Publication number: 20140066490
    Abstract: A system and method for programming DNAzymes to be utilized as programmable drugs, which are active only in the presence of specific input combinations and/or certain conditions.
    Type: Application
    Filed: August 14, 2013
    Publication date: March 6, 2014
    Applicant: YEDA Research and Development Co. Ltd.
    Inventors: Ehud Y. SHAPIRO, Maya KANAN-HANUM, Yehonatan DOUEK, Rivka ADAR
  • Publication number: 20140066337
    Abstract: The invention provides a method of printing, onto a substrate (12), an array (14) of spots of reagent compositions for use in a chemical and/or biochemical analysis. The method includes displacing an array of reagent composition containing capillary tubes (22) arranged alongside one another from an inoperative position to an operative position in which open ends of the capillary tubes (22) simultaneously impinge against a substrate and thereafter displacing the array of tubes (22) from the operative position back to the inoperative position. The invention extends to a printing apparatus (10), a method of printing a layered array of spots of reagent compositions, a method of introducing reagent compositions into the tubes, a reagent introducing device for introducing reagent compositions into the tubes and a printing installation which includes the printing apparatus (10) and the reagent introducing device.
    Type: Application
    Filed: November 8, 2013
    Publication date: March 6, 2014
    Inventor: Neil Emans
  • Patent number: 8663397
    Abstract: The embodiments describe methods for controlling the particles generated when cleaning and drying a wafer in a spin rinse dryer (SRD) module. In some embodiments, the substrate surface is cooled by dispensing deionized (DI) water across the surface of the substrate, while the substrate rests on the SRD chuck. In addition, a method for controlling the particles generated when sleeves in a processing module or SRD contact a substrate surface during a clamping operation or when the sleeves are removed from the substrate surface is provided. A bottom edge or lip of the sleeves and/or the surface of the wafer contacting the sleeve is wetted during clamping/unclamping operations. Alternatively, the substrate may be wetted prior to clamping/unclamping operations.
    Type: Grant
    Filed: October 22, 2012
    Date of Patent: March 4, 2014
    Assignee: Intermolecular, Inc.
    Inventors: Satbir Kahlon, Jeffrey Chih-Hou Lowe, Frank C. Ma, Sandeep Mariserla, Robert Anthony Sculac
  • Publication number: 20140051604
    Abstract: A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) (“aqueous phase”) flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow.
    Type: Application
    Filed: October 18, 2013
    Publication date: February 20, 2014
    Applicant: STOKES BIO LIMITED
    Inventors: Mark Davies, Tara Dalton, Julie Garvey, Kieran Curran, Damian Curtin
  • Publication number: 20140045704
    Abstract: The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.
    Type: Application
    Filed: May 2, 2013
    Publication date: February 13, 2014
    Applicant: IntegenX Inc.
    Inventors: Stevan Bogdan Jovanovich, luliu I. Balga, Allen R. Boronkay, Joanne Horn, Michael Van Nguyen, William D. Nielsen, Mattias Vangbo
  • Publication number: 20140038853
    Abstract: In a method and system for forming concentrated volumes of microbeads, a polymer solution and/or suspension includes a polymer dissolved and/or dispersed in a medium. Streams of a focusing fluid and of the polymer solution and/or suspension flow towards a fluid bath, and into intersection with one another, so as to focus the polymer solution and/or suspension. The polymer solution and/or suspension stream forms microbeads in the fluid bath Some of the focusing fluid is drawn from the fluid bath, so as to concentrate the microbeads in the fluid bath. The system includes a flow focusing apparatus and a liquid-containing cell. The focusing apparatus includes polymer and focusing nozzles. The cell contains the fluid bath and has an outlet port, through which the focusing fluid is drawn.
    Type: Application
    Filed: October 7, 2013
    Publication date: February 6, 2014
    Applicant: FIO CORPORATION
    Inventors: Sebastian Fournier-Bidoz, Warren Che Wor Chan
  • Publication number: 20140038854
    Abstract: The invention relates to a device and a method for the generation of molecular microarrays. The invention relates therefore to a universal approach for the generation of protein microarrays, DNA microarrays and RNA microarrays (in general nucleic acid microarrays), by production of an output molecule from a template molecule microarray via enzymatic or chemical processes and transfer of the output molecule onto the desired molecular microarray.
    Type: Application
    Filed: February 3, 2012
    Publication date: February 6, 2014
    Applicant: ALBERT-LUDWIGS-UNIVERSITAET FREIBURG
    Inventors: Günter Roth, Jügen Burger
  • Patent number: 8642516
    Abstract: Patterned SAM arrays and methods of preparing patterned SAM arrays are disclosed. Advantageously, the methods used to prepare the patterned SAM arrays allow for controlling SAM spot-to-spot conditions such as ligand identity and ligand density, which allows for preparing a wide range of SAM spots in a single array format. Additionally, the patterned SAM arrays of the present disclosure support the culture of a range of cell types. The patterned SAM arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.
    Type: Grant
    Filed: May 7, 2012
    Date of Patent: February 4, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Justin T. Koepsel, William L. Murphy
  • Publication number: 20140031262
    Abstract: An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.
    Type: Application
    Filed: September 30, 2013
    Publication date: January 30, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Tanya S. Kanigan, Steve Smith, John Linton, Robert Hess, Karl Yoder, Colin Brennan