For Creating A Library Patents (Class 506/40)
  • Patent number: 11951469
    Abstract: A device for storing reagent containers on several planes for an automatic analysis appliance comprises a pipetting device comprising a pipetting needle for pipetting of reagents, a first device comprising a plurality of first receiving positions for reagent containers, wherein the first receiving positions are arranged on a first plane, and a second device comprising a plurality of second receiving positions for reagent containers, wherein the second receiving positions are arranged on a second plane.
    Type: Grant
    Filed: June 4, 2020
    Date of Patent: April 9, 2024
    Assignee: Siemens Healthcare Diagnostic Products GmbH
    Inventor: Thorsten Michels
  • Patent number: 11931738
    Abstract: An example method includes providing fluid to a chamber. The chamber feeds a first channel terminating at a first droplet ejector and a second channel terminating at a second droplet ejector. The method further includes sequencing ejection of droplets at the first droplet ejector and the second droplet ejector to induce negative pressure to provide a sequenced output flow of the fluid through the first channel to a first target microfluidic network and through the second channel to a second target microfluidic network, and controlling the first and second target microfluidic networks to perform an analytical process with the fluid.
    Type: Grant
    Filed: November 22, 2018
    Date of Patent: March 19, 2024
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: John Lahmann, Pavel Kornilovich, Silam J Choy
  • Patent number: 11913067
    Abstract: A composition for amplifying a polynucleotide is provided that includes a substrate comprising a first region and a second region. A first plurality of capture primers is coupled to the first region of the substrate. A second plurality of capture primers is coupled to the second region of the substrate. The capture primers of the second plurality of capture primers are longer than the capture primers of the first plurality of capture primers. A first plurality of orthogonal capture primers are coupled to the first region of the substrate. A second plurality of orthogonal capture primers are coupled to the second region of the substrate. The orthogonal capture primers of the second plurality of orthogonal capture primers are shorter than the orthogonal capture primers of the first plurality of orthogonal capture primers.
    Type: Grant
    Filed: August 27, 2021
    Date of Patent: February 27, 2024
    Assignees: Illumina, Inc., Illumina Cambridge Limited
    Inventors: Jeffrey Fisher, Jason Betley
  • Patent number: 11911738
    Abstract: The system can include: a conduit having a first dimension with an inlet and outlet; an extruder having an inlet and an outlet located within the conduit, an extruder orifice having a second dimension that is smaller than the first dimension; a carrier fluid reservoir coupled with the conduit inlet; an extruder reservoir coupled with the extruder inlet; and a particle collector fluidly coupled with the conduit outlet, wherein the particle collector has a collector inlet with a first temperature and a collector outlet with a second temperature. The method can include flowing carrier fluid through the extrudate conduit; extruding wax with the extruder into the carrier fluid that is flowing through the extrudate conduit such that the extrudate separates into extrudate segments separated from each other by carrier fluid segments; and flowing the extrudate into the particle collector so as to form wax particles.
    Type: Grant
    Filed: August 30, 2022
    Date of Patent: February 27, 2024
    Assignee: MATRALIX PTE LTD
    Inventors: Siew Keong Loke, Kit Ying Soo
  • Patent number: 11904322
    Abstract: The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). Methods and systems provided herein may facilitate performing reactions such as emulsion PCR (ePCR) on samples comprising nucleic acids and beads. The methods provided herein may provide a higher throughput compared to existing technologies.
    Type: Grant
    Filed: February 16, 2022
    Date of Patent: February 20, 2024
    Inventors: Jose Martin Sosa, Joseph Anthony, Nathan Beckett, Gilad Almogy, Chandan Shee, Phillip You Fai Lee, Yuto Watanabe
  • Patent number: 11846580
    Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
    Type: Grant
    Filed: March 3, 2021
    Date of Patent: December 19, 2023
    Assignees: Illumina, Inc., Illumina Cambridge Limited
    Inventors: James Tsay, Anmiv Prabhu, David Heiner, Edwin Li, Alexandre Richez, John M. Beierle, Kevan Samiee, Kristina Munoz, Leonid Malevanchik, Ludovic Vincent, Naiqian Zhan, Peyton Shieh, Robert Yang, Samantha Schmitt, Sang Park, Scott Bailey, Sean M. Ramirez, Sunmin Ahn, Valerie Uzzell, Wei Wei, Yuxiang Huang, Tyler Jamison Dill
  • Patent number: 11840757
    Abstract: A film deposition system according to the present embodiment includes a film deposition apparatus, and a computer, in which the film deposition apparatus includes a film deposition chamber in which a plurality of deposition species are installable, and the computer includes a calculation region that calculates based on a calculation model having an Ising model or QUBO, and predict a time required for film deposition when a disposition of the deposition species is set.
    Type: Grant
    Filed: June 15, 2021
    Date of Patent: December 12, 2023
    Inventors: Tsuyoshi Suzuki, Katsuyuki Nakada, Tomoyuki Sasaki
  • Patent number: 11833515
    Abstract: Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.
    Type: Grant
    Filed: April 27, 2020
    Date of Patent: December 5, 2023
    Assignee: 10x Genomics, Inc.
    Inventors: Tobias Daniel Wheeler, Bill Kengli Lin, Mohammad Rahimi Lenji, Anthony Makarewicz, Rajiv Bharadwaj
  • Patent number: 11794188
    Abstract: A combination micro/macro-fluidic analysis system with one or more of a droplet transition unit, a droplet cleaving unit, a droplet synchronization, and a merging unit to enable highly efficient complex droplet microfluidic assays.
    Type: Grant
    Filed: April 23, 2021
    Date of Patent: October 24, 2023
    Inventors: Arum Han, Adrian Guzman, Han Zhang, Jose Wippold, Jing Dai
  • Patent number: 11770885
    Abstract: Apparatuses of various forms and each having at least one LED string controlled by a current controller biased by voltage tap nodes in the LED string, and circuitry for implementing the same. In some implementations, circuitry made in accordance with the present disclosure includes a current controller and current-control circuitry. The current controller is biased by a voltage drop across one or more LEDs in an LED string and controls the current-control circuitry in a manner that controls the electrical current in the LED string. In some implementations, one or more additional LED strings and/or one or more other devices may be controlled directly or indirectly by operation of the current controller.
    Type: Grant
    Filed: October 26, 2020
    Date of Patent: September 26, 2023
    Inventors: Jonathan Williams, Jose Garcia, Guruprakash Iyer
  • Patent number: 11754527
    Abstract: An aspect of the invention pertains to an affinity biosensor for sensing an analyte (a biomolecule) in a fluid, comprising an interface for contacting the fluid and adsorption of the analyte. The interface comprises a binary pattern of nanoscale regions having affinity for the analyte and a passivated region. The nanoscale regions are isolated from one another by the passivated region in such a way that adsorption of the analyte on the interface is confined to the nanoscale regions. The nanoscale regions have diameters comprised in the range from 5 to 200 nm. The nanoscale regions have together a surface area amounting to at least 15% of the surface area of the interface. A further aspect of the invention relates to a method of using such a sensor.
    Type: Grant
    Filed: August 18, 2020
    Date of Patent: September 12, 2023
    Inventors: Sivashankar Krishnamoorthy, Matteo Beggiato
  • Patent number: 11698596
    Abstract: A fixing device is for fixing a developing agent image to a recording sheet by electrostatically spraying a charged fixing solution toward the developing agent image on the sheet. The fixing device includes a container portion, a plurality of nozzles, and a potential difference generating portion. The container portion is configured to store therein the fixing solution. The plurality of nozzles is in communication with the container portion and configured to spray the fixing solution toward the developing agent image. The potential difference generating portion is configured to generate a potential difference between the fixing solution stored in the plurality of nozzles and the recording sheet conveyed at a position separated from the plurality of nozzles.
    Type: Grant
    Filed: May 13, 2021
    Date of Patent: July 11, 2023
    Assignee: Brother Kogyo Kabushiki Kaisha
    Inventors: Yutaka Kakigahara, Kengo Takeda, Jun Mihara, Satoshi Murata, Shinya Yamamoto, Tomoaki Hattori, Kumiko Sakaguchi, Takayuki Higuchi, Tatsuya Ezaka, Emi Shimizu, Kentaro Murayama
  • Patent number: 11697668
    Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.
    Type: Grant
    Filed: April 21, 2020
    Date of Patent: July 11, 2023
    Inventors: Pierre F. Indermuhle, Eugene P. Marsh, Andres Fernandez, William Banyai, Bill J. Peck
  • Patent number: 11679384
    Abstract: Embodiments are directed towards methods and systems of depositing a uniform test-pathogen mixture onto a test article for testing the sterilization efficacy of an electromagnetic radiation or other sterilization process. The system includes a holding mechanism configured to removably secure the test article to the system. The system also includes a test-pathogen dispenser configured to uniformly deposit the test-pathogen mixture onto a reference surface of the test article. The system is structured so that at least one of the test article and the test-pathogen dispenser moves relative to the other. A plurality of test-pathogen mixture droplets or lines is deposited onto the reference surface in a predetermined test-pathogen pattern, such as, for example, a plurality of rows and columns of droplets. A distance from a dispenser tip of the test-pathogen dispenser to the reference surface of the test article may be determined to help maintain consistency between test-pathogen mixture droplets or lines.
    Type: Grant
    Filed: July 21, 2017
    Date of Patent: June 20, 2023
    Assignee: GERMITEC
    Inventor: Adrian Edward Smith
  • Patent number: 11646100
    Abstract: Embodiments of a method and/or system for facilitating characterization of one or more conditions can include: generating a set of target-associated molecules; generating a reference-associated set of molecule; facilitating generation of at least one spike-in mixture; determining one or more abundance metrics based on an analysis of the at least one spike-in mixture; and facilitating the characterization of the one or more conditions based on the one or more abundance metrics.
    Type: Grant
    Filed: August 6, 2018
    Date of Patent: May 9, 2023
    Assignee: BILLIONTOONE, INC.
    Inventors: David Tsao, Oguzhan Atay
  • Patent number: 11524291
    Abstract: A fluidic device (10) is described. The fluidic device (10) comprises the first part (110) and the second part (120). The first part (110) comprises a first inlet (111) and a first outlet (112), mutually spaced apart. The second part (120) comprises a first chamber (121) arranged to contain a predetermined first amount A1 of a first fluid F1 therein and a first wall portion (122) arranged to contain, at least in part, the first fluid F1 in the first chamber (121). The fluidic device (10) is arrangeable in a first configuration, wherein the first part (110) is fluidically isolated from the first chamber (121). The fluidic device (10) is arrangeable in a second configuration, wherein the first inlet (111) and the first outlet (112) are fluidically coupled via the first chamber (121), whereby increasing a first pressure P1 in the first chamber (121) via the first inlet (111) urges at least a part of the predetermined first amount A1 of the first fluid F1 through the first outlet (112).
    Type: Grant
    Filed: March 29, 2019
    Date of Patent: December 13, 2022
    Inventors: Maiwenn Kersaudy-Kerhoas, Antonio Liga
  • Patent number: 11255011
    Abstract: A mask structure for a deposition device includes first segments and second segments. The first segments are arranged in a direction surrounding a central axis and separated from one another. The second segments are disposed above the first segments. Each of the second segments overlaps two of the first segments adjacent to each other in a vertical direction parallel to an extending direction of the central axis. A deposition device includes a process chamber, a stage, and the mask structure. The stage is at least partially disposed in the process chamber and includes a holding structure of a substrate. The mask structure is disposed in the process chamber, located over the stage, and covers a peripheral region of the substrate to be held on the stage. An operation method of the deposition device includes horizontally adjusting positions of the first segments and the second segments respectively between different deposition processes.
    Type: Grant
    Filed: September 17, 2020
    Date of Patent: February 22, 2022
    Assignee: United Semiconductor Japan Co., Ltd.
    Inventor: Satoshi Inagaki
  • Patent number: 11229909
    Abstract: An apparatus includes a flow cell body, a plurality of electrodes, an integrated circuit, and an imaging assembly. The flow cell body defines one or more flow channels and a plurality of wells. Each flow channel is configured to receive a flow of fluid. Each well is fluidically coupled with the corresponding flow channel. Each well is configured to contain at least one polynucleotide. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are operable to effect writing of polynucleotides in the corresponding wells. The integrated circuit is operable to drive selective deposition or activation of selected nucleotides to attach to polynucleotides in the wells to thereby generate polynucleotides representing machine-written data in the wells. The imaging assembly is operable to capture images indicative of one or more nucleotides in a polynucleotide.
    Type: Grant
    Filed: May 26, 2020
    Date of Patent: January 25, 2022
    Assignee: ILLUMINA, INC.
    Inventors: Ali Agah, Aathavan Karunakaran, Tarun Khurana, Stanley Hong, Merek Siu, Arvin Emadi, Craig Ciesla
  • Patent number: 10760936
    Abstract: The present disclosure provides a semiconductor device. The semiconductor device includes a substrate, a pair of walls and a conductive layer. The pair of walls, disposed on the substrate, are configured to define a recess therebetween to receive a liquid. The conductive layer is disposed above the substrate, and has a resistance, wherein the resistance is correlated with a surface tension of the liquid in the recess.
    Type: Grant
    Filed: November 2, 2018
    Date of Patent: September 1, 2020
    Inventor: Chung-Peng Hao
  • Patent number: 9855555
    Abstract: The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: January 2, 2018
    Assignee: University of Maryland
    Inventors: Ian M. White, Srinivasa Raghavan, Kunal R. Pandit
  • Patent number: 9267170
    Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: February 23, 2016
    Assignee: Life Technologies Corporation
    Inventors: Ronald Danehy, Gary Lim, Jacob Freudenthal
  • Patent number: 9157161
    Abstract: A thin film with multiple binding functionality can be prepared on an electrode surface via consecutive electroreduction of two or more aryl-onium salts with different functional groups. This versatile and simple method for forming multifunctional surfaces provides an effective means for immobilization of diverse molecules at close proximities. The multifunctional thin film has applications in bioelectronics, molecular electronics, clinical diagnostics, and chemical and biological sensing.
    Type: Grant
    Filed: July 14, 2011
    Date of Patent: October 13, 2015
    Assignee: Sandia Corporation
    Inventors: Susan M. Brozik, Jason C. Harper, Ronen Polsky, David R. Wheeler, Dulce C. Arango, Shawn M. Dirk
  • Patent number: 9085461
    Abstract: A wafer having a plurality of dies (also called array chips) on the wafer, the die having an electrode to generate a deprotecting reagent, a working electrode to electrochemically synthesize a material, a confinement electrode adjacent to the working electrode to confine reactive reagents, and a die pad, wherein die pads of the plurality of dies are interconnected on the wafer to electrochemically synthesize the material in parallel on a plurality of working electrodes is disclosed. Also, a method for wafer-scale manufacturing of a plurality of dies and a method for electrochemically synthesizing a material in parallel on a plurality of dies on a wafer are disclosed.
    Type: Grant
    Filed: May 16, 2012
    Date of Patent: July 21, 2015
    Inventors: Valery M. Dubin, Florian Gstrein, Gordon D. Holt, Brandon Barnett
  • Publication number: 20150148238
    Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 28, 2015
    Applicants: ADVANCED LIQUID LOGIC, INC., Duke University
    Inventors: Vamsee Pamula, Vijay Srinivasan, Michael G. Pollack, Richard B. Fair
  • Publication number: 20150148258
    Abstract: The present invention describes a method and devices for the simultaneous detection, recovery identification and counting of a plurality of microorganisms consisting in providing mixtures of nutrients specially selected from those that curtail the lag phase of growth in bacteria and moulds and which, together with fluorescent enzymatic, chromogenic or bioluminescent markers and other nutrient components or growth inhibitors, are embedded in three-dimensional structures or natural or artificial clays or ceramics with cavities of different dimensions and forms and specific surface areas of between 2×103 and 6×108 m2/m3.
    Type: Application
    Filed: March 27, 2013
    Publication date: May 28, 2015
    Inventors: Claudio Rodriguez Martinez, Jesus Eduardo Gonzalez Ruiz, Tamara Lobaina Rodriguez, Raisa Zhurbenko, Ana Iris Brito Gonzales, Monica Lopez Hernandez, Javier Fernandez Aragon, Ivonne Alfonso Valdes, Adelaida Ortea Suris
  • Publication number: 20150141261
    Abstract: The present disclosure generally relates to sequencing two or more genes expressed in a single cell in a high-throughput manner. More particularly, the present disclosure relates to a method for high-throughput sequencing of pairs of transcripts co expressed in single cells (e.g., antibody VH and VL coding sequence) to determine pairs of polypeptide chains that comprise immune receptors.
    Type: Application
    Filed: June 17, 2013
    Publication date: May 21, 2015
    Inventors: Scott Hunicke-Smith, Brandon Dekosky, Andy Ellington, George Georgiou
  • Publication number: 20150126411
    Abstract: The systems and methods described herein relate to a high-throughput flow apparatus. The apparatus is used with an array of wells, and is configured to impart a predetermined shear stress on cells cultured within each of the wells of the array of wells. The apparatus includes a plurality of mechanical tips. The plurality of mechanical tips each includes a head with a hemispheroid shape. The apparatus also includes a motor associated with at least one of plurality of mechanical tips. The motor is configured to drive the plurality of mechanical tips to impart the shear stress pattern in each of the wells.
    Type: Application
    Filed: November 6, 2014
    Publication date: May 7, 2015
    Inventors: Guillermo Garcia-Cardena, Peter Mack, Jeffrey T. Borenstein, Ahmad S. Khalil, Eli J. Weinberg, Jason O. Fiering, Ernest S. Kim, William J. Adams, JR., Mitchell Hansberry, Stephen Bellio
  • Publication number: 20150126412
    Abstract: A method and an array filling system for loading a plurality of disparate sample containers, the sample containers comprising an integral structure. Each receptacle is characterized by a hydrophilic surface, and the receptacles are separated by a hydrophobic surface. The system has a liquid transfer device capable of holding liquid and adapted for motion to cause sequential communication of liquid held in the liquid transfer device with successive receptacles of the array by dragging the liquid across the hydrophobic surface.
    Type: Application
    Filed: June 10, 2014
    Publication date: May 7, 2015
    Inventors: Ian Hunter, Colin J.H. Brenan
  • Publication number: 20150119274
    Abstract: The present invention describes microplates with arrays of wells containing magnetic inserts in the form of disks, cylinders or otherwise shaped materials with apertures in the centre for optical interrogation of the contacting solution. These inserts are capable of capturing ferro- and paramagnetic nano- and microparticles. The subject microplates find use in a variety of applications, including clinical and environmental assays, high throughput screening for genomics, proteomics and pharmaceutical applications, point-of-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally and high-throughput screening of materials for separation and catalysis.
    Type: Application
    Filed: June 3, 2013
    Publication date: April 30, 2015
    Inventors: Sergey Anatoliyovich Piletsky, Olena Piletska, Antonio Guerreiro, Alessandro Poma, Khalku Karim, Stanislav Piletsky
  • Publication number: 20150111773
    Abstract: The present invention provides methods for rapidly screening and measuring the ligand binding affinity of in vitro selected peptides to the cognate and off-target proteins. This general strategy is amenable to high throughput analysis because the peptides are synthesized by cell-free translation, as opposed to solid-phase synthesis required by traditional assays, and affinities can be readily measured in standard formats.
    Type: Application
    Filed: June 7, 2013
    Publication date: April 23, 2015
    Inventors: John Chaput, Andrew Larsen, Annabelle Gillig
  • Patent number: 8999726
    Abstract: Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter may be used to deposit highly concentrated spots of protein or other materials on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells, microfluidic conduits, and orifices to deposit proteins, other biomolecules, or chemicals on a spot on, a separate surface. Each orifice is connected to two wells via microconduits. When the spotter contacts a surface, a seal is formed between the orifices and the surface. The same or different substances may be flowed across each orifice. Any number of orifices may be incorporated into a spotter.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: April 7, 2015
    Assignee: University of Utah Research Foundation
    Inventors: David A. Chang-Yen, Sriram Natarajan, Josh Eckman, Bruce K. Gale, David Myszka
  • Patent number: 8999266
    Abstract: A slotted pin tool, a delivery system containing the pin tool, a substrate for use in the system and methods using the pin tool and system are provided. The slotted pin tool contains a plurality of pins having slotted ends designed to fit around each loci of material deposited on a surface, such as a microarray, without contacting any of the deposited material. Sample is delivered by contacting the pin tool with the surface; the amount delivered is proportional to the velocity of the pin tool as it contacts the surface or the velocity of the liquid when movement of the pin is halted.
    Type: Grant
    Filed: November 21, 2012
    Date of Patent: April 7, 2015
    Assignee: Agena BioScience, Inc.
    Inventors: Xian-Wei Yao, Chao Lin, Paul Heaney, Thomas Becker, Aaron A. Hanson, Michael C. Willis
  • Publication number: 20150094238
    Abstract: A system for inserting reagent or macrobeads into bores of a sample plate is disclosed comprising an insertion device arranged to retain one or more reagent or macrobeads by means of a vacuum whilst inserting the reagent or macrobeads into bores of the sample plate.
    Type: Application
    Filed: March 6, 2013
    Publication date: April 2, 2015
    Applicant: Dynex Technologies, Inc.
    Inventors: Adrian Bunce, Andrew Fusellier
  • Publication number: 20150072897
    Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85+/?15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.
    Type: Application
    Filed: March 15, 2013
    Publication date: March 12, 2015
    Inventors: Michael C. Pallas, James C. Nurse, Gary Lim, Theodore E. Straub, Evan W. Foster
  • Publication number: 20150072900
    Abstract: Sample processing droplet actuators, systems and methods are provided. According to one embodiment, a stamping device including a droplet microactuator is provided and includes: (a) a first plate including a path or network of control electrodes for transporting droplets on a surface thereof; (b) a second plate mounted in a substantially parallel orientation with respect to the first plate providing an interior volume between the plates, the second plate including one or more stamping ports for transporting some portion or all of a droplet from the interior volume to an exterior location; (c) a port for introducing fluid into the interior volume between the plates; and (d) a path or network of reference electrodes corresponding to the path or network of control electrodes. Associated systems and methods including the stamping device are also provided.
    Type: Application
    Filed: September 26, 2014
    Publication date: March 12, 2015
    Inventors: Vijay Srinivasan, Vamsee K. Pamula, Michael G. Pollack
  • Publication number: 20150057184
    Abstract: Provided is a microarray platform for the culture of cells atop combinatorial matrix mixtures; enabling the study of differentiation in response to a multitude of microenvironments in parallel.
    Type: Application
    Filed: March 25, 2014
    Publication date: February 26, 2015
    Inventors: Sangeeta N. BHATIA, Christopher FLAIM
  • Patent number: 8961899
    Abstract: The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.
    Type: Grant
    Filed: June 24, 2013
    Date of Patent: February 24, 2015
    Assignee: IBIS Biosciences, Inc.
    Inventors: Mark Eshoo, John Picuri
  • Publication number: 20150051118
    Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 19, 2015
    Inventors: Eliodor Ghenciu, Theodore E. Straub, Evan W. Foster, Michael C. Pallas
  • Publication number: 20150038379
    Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
    Type: Application
    Filed: October 10, 2014
    Publication date: February 5, 2015
    Inventors: Emil P. KARTALOV, Darryl SHIBATA, Clive TAYLOR, Lawrence A. WADE
  • Publication number: 20150038377
    Abstract: A liquid dispenser for a microfluidic assay system is described. The dispenser includes at least one transfer pin for transferring a microfluidic sample of liquid to a target receptacle. A pin tip at one end of the transfer pin is structured to cooperate with an opening in the target receptacle. The tip uses a high voltage potential to transfer the sample from the pin to the receptacle.
    Type: Application
    Filed: July 15, 2014
    Publication date: February 5, 2015
    Inventors: Leila Madrone, John Linton, Colin John Herbert Brenan
  • Publication number: 20150018248
    Abstract: Methods and systems for processing a first solution on a respective primary area of a device with a plurality of primary areas and a plurality of secondary areas are described. A method includes providing the device and dispensing a second solution to a respective secondary area of one or more secondary areas adjacent to the respective primary area, thereby mixing the first solution on the respective secondary area and the second solution on the respective primary area. The method also includes removing at least a portion of the mixed solution.
    Type: Application
    Filed: July 9, 2014
    Publication date: January 15, 2015
    Inventor: Namyong KIM
  • Patent number: 8927465
    Abstract: A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray.
    Type: Grant
    Filed: August 3, 2007
    Date of Patent: January 6, 2015
    Assignee: National University University of Singapore
    Inventors: Dieter Trau, Wen-Tso Liu, Kian Kok Johnson Ng
  • Patent number: 8920752
    Abstract: Novel and improved systems and methods for high speed arraying, hybridization, quantitative development and/or assaying are provided. Some embodiments provide a web based arraying format. Some other embodiments provide a sheet based arraying format. Some embodiments use a drop on drop assaying or hybridization mode. In some embodiments, a substantially inert substrate is utilized. In some other embodiments, an interactive substrate is utilized.
    Type: Grant
    Filed: February 1, 2013
    Date of Patent: December 30, 2014
    Assignee: Biodot, Inc.
    Inventors: Thomas C Tisone, Holger Eickhoff
  • Publication number: 20140378348
    Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In some embodiments, the system may include a well and a channel component attached to the well. The channel component may include (a) a body, (b) an input tube (a “fluid pickup”) projecting from a bottom surface of the body and having an open bottom end disposed in the input well, (c) a microchannel, and (d) a passage extending through the input tube and the body and connecting the well to the microchannel. The system may be configured to receive a sample-containing fluid in the well and retain the sample-containing fluid below a top end of the passage, until a pressure differential is created that drives at least a portion of the sample-containing fluid from the well via the passage and through the microchannel.
    Type: Application
    Filed: June 23, 2014
    Publication date: December 25, 2014
    Inventors: Anthony J. Makarewicz, JR., Luc Bousse, Stefano Schiaffino
  • Publication number: 20140371107
    Abstract: Methods, devices and systems for handling sample liquids, encapsulating liquids and magnetic particles are disclosed.
    Type: Application
    Filed: January 25, 2013
    Publication date: December 18, 2014
    Inventors: Kieran Curran, David McGuire
  • Publication number: 20140336084
    Abstract: A substrate, which is useful for performing biological, chemical and diagnostic assays, and a method for preparing the substrate are provided. The substrate has an upper surface with a coating disposed thereon. The coating comprises a charged polymer, a non-ionic polyether, and a silicate compound. The substrate can increase capture phase binding and reduce non-specific binding of biomolecules for a biological microarray.
    Type: Application
    Filed: December 20, 2012
    Publication date: November 13, 2014
    Inventors: Michael J. Pugia, Pratap Singh
  • Publication number: 20140274808
    Abstract: The present invention provides a system for receiving biological sequence information and activating the synthesis of a biological entity. The system has a receiving unit for receiving a signal encoding biological sequence information transmitted from a transmitting unit. The transmitting unit can be present at a remote location from the receiving unit. The system also has an assembly unit connected to the receiving unit, and the assembly unit assembles the biological entity according to the biological sequence information. Thus, according to the present invention biological sequence information can be digitally transmitted to a remote location and the information converted into a biological entity, for example a protein useful as a vaccine, immediately upon being received by the receiving unit and without further human intervention after preparing the system for receipt of the information.
    Type: Application
    Filed: August 16, 2013
    Publication date: September 18, 2014
    Applicant: Synthetic Genomics, Inc.
    Inventors: J. Craig Venter, Daniel Gibson, John E. Gill
  • Publication number: 20140274809
    Abstract: A multi-well manifold assembly and method for reducing cross-contamination in continuous synthesis reactions in channels of microfluidic devices, for example oligonucleotide synthesis.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Inventors: Jon Harvey, Ryan Witt, William E. Martin, III
  • Publication number: 20140249057
    Abstract: The present teachings provide a fluid processing device adapted to produce different oligomers in a plurality of respective reaction sites. The fluid processing device can comprise a first manifold for delivering reactants to the plurality of reaction sites, and a second manifold for removing waste from, and optionally delivering wash fluid to, the plurality of reaction sites. Surface tension control valves can be disposed in fluid communication with the first manifold and can selectively allow reactants and/or fluids into the reaction sites. A method of making oligonucleotides is also provided.
    Type: Application
    Filed: January 31, 2014
    Publication date: September 4, 2014
    Inventor: Sergey V. Ermakov
  • Publication number: 20140235485
    Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.
    Type: Application
    Filed: February 18, 2014
    Publication date: August 21, 2014
    Inventors: Steven Gordon, Daniel Stetson