Link Or Spacer Specially Adapted For Combinatorial Chemistry Or A Library (e.g., Traceless Linker, Safety-catch Linker, Etc.) Patents (Class 506/42)
  • Patent number: 9598720
    Abstract: The present invention concerns monomeric or polymeric linker molecules useful in biological and chemical applications, their synthesis, and the synthesis and use of derivatives of the linkers conjugated to a variety of detectable labels and other substances. The linkers may be used, for example, in conjunction with fluorescent labels, nucleic acid or nucleic acid analog probes, and solid phase systems, and to enhance the solubility of the conjugated molecules.
    Type: Grant
    Filed: August 21, 2013
    Date of Patent: March 21, 2017
    Assignee: DAKO DENMARK A/S
    Inventor: Jesper Lohse
  • Patent number: 8906847
    Abstract: The present invention relates to a prodrug or a pharmaceutically acceptable salt thereof comprising a drug linker conjugate D-L, wherein -D is an amine containing biologically active moiety; and -L is a non-biologically active linker moiety -L1 represented by formula (I), wherein the dashed line indicates the attachment to the amine of the biologically active moiety and wherein R1, R1a, R2, R2a, R3, R3a, X, X1, X2, X3 have the meaning as indicated in the description and the claims and wherein L1 is substituted with one to four groups L2-Z and optionally further substituted, provided that the hydrogen marked with the asterisk in formula (I) is not replaced by a substituent; wherein L2 is a single chemical bond or a spacer; and Z is a carrier group. The invention also relates to A-L, wherein A is a leaving group, pharmaceutical composition comprising said prodrugs and their use as medicaments.
    Type: Grant
    Filed: January 30, 2009
    Date of Patent: December 9, 2014
    Assignee: Ascendis Pharma A/S
    Inventors: Felix Cleemann, Ulrich Hersel, Silvia Kaden, Harald Rau, Thomas Wegge
  • Publication number: 20140221254
    Abstract: Disclosed herein are compositions, methods and kits for analyzing three-dimensional chromatin and/or chromosome conformation. Method are also disclosed for using the methods disclosed herein for diagnosing diseases such as cancer.
    Type: Application
    Filed: December 23, 2013
    Publication date: August 7, 2014
    Inventors: Shoulian DONG, Junko F. STEVENS, Chunmei LIU, Luz MONTESCLAROS
  • Patent number: 8779221
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Grant
    Filed: May 22, 2012
    Date of Patent: July 15, 2014
    Assignee: Promega Corporation
    Inventors: Aldis Darzins, Lance Encell, Tonny Johnson, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • Patent number: 8759259
    Abstract: Embodiments of the invention are directed to a one-bead-two-compound method for the creation of encoded cyclic peptoid libraries. This scheme is useful for the creation of cyclic peptoid microarrays since only the cyclic peptoid, not the linear encoding molecule, contains an attachment residue and thus can be spotted onto an activated substrate.
    Type: Grant
    Filed: October 15, 2010
    Date of Patent: June 24, 2014
    Assignee: The Board of Regents of the University of Texas System
    Inventors: Yong-Uk Kwon, Thomas Kodadek
  • Patent number: 8680020
    Abstract: Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis.
    Type: Grant
    Filed: July 15, 2009
    Date of Patent: March 25, 2014
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Chung-Yi Wu, Susan Y. Tseng
  • Publication number: 20130296173
    Abstract: The present invention is directed to compositions and methods for improving the discordance rate and mapping yield in nucleic acid sequencing reactions.
    Type: Application
    Filed: April 22, 2013
    Publication date: November 7, 2013
    Inventors: Matthew Callow, Linsu Chen, Dennis Ballinger
  • Patent number: 8546309
    Abstract: The present invention concerns monomeric or polymeric linker molecules useful in biological and chemical applications, their synthesis, and the synthesis and use of derivatives of the linkers conjugated to a variety of detectable labels and other substances. The linkers may be used, for example, in conjunction with fluorescent labels, nucleic acid or nucleic acid analog probes, and solid phase systems, and to enhance the solubility of the conjugated molecules.
    Type: Grant
    Filed: June 30, 2006
    Date of Patent: October 1, 2013
    Assignee: Dako Denmark A/S
    Inventor: Jesper Lohse
  • Patent number: 8415277
    Abstract: Provided are a bi-functional compound that is positively charged at a first pH and negatively charged at a second pH, a solid support having the bi-functional compound immobilized thereon, and a method of isolating a nucleic acid, including: binding the bi-functional compound with a nucleic acid at a first pH and isolating the nucleic acid from the bi-functional compound at a second pH.
    Type: Grant
    Filed: September 8, 2009
    Date of Patent: April 9, 2013
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Kyu-youn Hwang, Joon-ho Kim
  • Publication number: 20120322669
    Abstract: Methods for marking a material and subsequently detecting that it has been marked, comprising adding or applying a marker comprising a nucleic acid tag to the material, sampling a portion of the material, and detecting the presence of the tag. The quantity of the tag present in the sample provides an indication of the quantity of marker present in the material. The tag may comprise one or more identifying sequences, and the tag is amplified by means of a nucleic acid amplification reaction.
    Type: Application
    Filed: July 13, 2012
    Publication date: December 20, 2012
    Inventors: John Edward Minton, Robert Sleat
  • Publication number: 20120295794
    Abstract: Compositions, methods, and kits for detecting one or more species of RNA molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the RNA molecule using a polypeptide comprising double-strand specific RNA ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the reverse transcription product are removed. Primers are added and amplified products are generated. In certain embodiments, the sequence of at least part of at least one species of amplified product is determined and at least part of the corresponding RNA molecule is determined. In some embodiments, at least some of the amplified product species are detected, directly or indirectly, allowing the presence and/or quantity of the RNA molecule of interest to be determined.
    Type: Application
    Filed: May 3, 2012
    Publication date: November 22, 2012
    Inventor: R. Scott KUERSTEN
  • Publication number: 20120178640
    Abstract: A composition can include a nanostructure, and a linker associated with the nanostructure, wherein the linker is configured to interact with a capture protein. The nanostructure can include a single-walled carbon nanotube. A plurality of the compositions can be configured in an array.
    Type: Application
    Filed: August 31, 2011
    Publication date: July 12, 2012
    Applicant: Massachusetts Institute of Technology
    Inventors: Michael S. Strano, Jin-Ho Ahn, Jong-Ho Kim, Paul W. Barone, Nigel F. Reuel
  • Patent number: 8133985
    Abstract: The present invention relates to a peptide nucleic acid (PNA) conjugated with multi-amine linkers, and a method to prepare the same and utilization thereof. More specifically, the method is characterized by conjugating monomers having multi-amine functionality sequentially at a PNA terminal, and effectively immobilizing the PNA conjugated with multi-amine linkers on a solid surface. A PNA array prepared using the PNA conjugated with multi-amine linkers exhibits improved sensitivity and specificity of signals for detecting target nucleic acids as compared to a PNA array using PNA probes having only one amine group. The PNA conjugated with multi-amine linkers can be utilized in nucleic acid detecting devices or kits for gene diagnosis such as PNA microarrays, PNA chips, PNA field-effect transistors and impedance detectors.
    Type: Grant
    Filed: December 14, 2007
    Date of Patent: March 13, 2012
    Assignee: Panagene Inc.
    Inventors: Hyunil Lee, Hee Kyung Park, Bong Ho Um, Serka Kim, Jae Jin Choi, Jung Hyun Min, Hye Yeon Kim
  • Publication number: 20120010086
    Abstract: Disclosed is a process for clonal pre-amplification of a nucleic acid involving the steps of (i) providing a plurality of different nucleic acid molecules (b) attaching adaptor sequences to the 3? ends and 5? ends of the nucleic acid molecules (c) preparing a water in oil emulsion wherein the majority of water droplets comprises one or none member of the plurality of different nucleic acid molecules (d) clonally amplifying the plurality of different nucleic acid molecules. In particular, the different nucleic acid molecules are mRNA molecules.
    Type: Application
    Filed: June 27, 2011
    Publication date: January 12, 2012
    Inventors: Thomas Froehlich, Tobias Heckel
  • Patent number: 7976824
    Abstract: The invention relates to a compound of formula (I): wherein P1, P2, P3, and P4 are each independently hydrogen or a protecting group; and n is an integer of from 2 to 20 and to the use of such compounds for the synthesis of 18F-FDG.
    Type: Grant
    Filed: July 29, 2004
    Date of Patent: July 12, 2011
    Assignee: GE Healthcare Limited
    Inventors: Lynda Jane Brown, Richard Charles Downie Brown, Harry John Wadsworth, Alexander Jackson
  • Patent number: 7858825
    Abstract: The invention provides compounds that are useful as linkers for solid phase synthesis and as protecting groups, and methods for producing and using the same.
    Type: Grant
    Filed: February 15, 2008
    Date of Patent: December 28, 2010
    Assignee: Colorado State University Research Foundation
    Inventors: Michio Kurosu, Dean Crick
  • Patent number: 7838261
    Abstract: A method for biochemical analysis uses a micro-reaction array with at least two reaction chambers for materials which react together chemically or biochemically. The reaction chambers are smaller than 1 ?l, the reaction chambers are filled together by throughflow, the chemical or biochemical reactions of the substances retained therein then occurs in the individual isolated reaction chambers, thus preventing an interference between the reactions in the individual reaction chambers and the reaction products remain enclosed in the relevant reaction chambers. In the system the planar array has at least two reaction chambers for substances, whereby the reaction chambers are closed with the goal of preventing an exchange of substances.
    Type: Grant
    Filed: November 26, 2001
    Date of Patent: November 23, 2010
    Assignee: Siemens Aktiengesellschaft
    Inventors: Walter Gumbrecht, Rainer Hintsche, Konrad Mund, Manfred Stanzel
  • Patent number: 7700524
    Abstract: Activated Supports, support-bound activators, strongly acidic supports, and silylating supports are provided; the activated support having the formula (I) wherein L is a linking group component; X is F, CL, OH, and trisubstituted silyloxy; and the shaded circle represents a solid or semi-solid support. Methods of using the activated supports in solid phase organic sync) thesis are also provided.
    Type: Grant
    Filed: January 11, 2002
    Date of Patent: April 20, 2010
    Assignee: GE Healthcare Limited
    Inventors: Christopher Holmes, Yijun Pan
  • Publication number: 20090239770
    Abstract: A detection oligomer and method for controlling the quality of a biochip using the detection oligomer are provided. The detection oligomer includes a template having a first and second end and having a sequence that is complementary to a specific sequence of an oligomer, and a hairpin connected to the first end of the template.
    Type: Application
    Filed: January 23, 2009
    Publication date: September 24, 2009
    Applicant: Samsung Electronics Co., Ltd
    Inventors: Joo-Won RHEE, Byung-chul Kim, Sun-Ok Jung, Seung-Hei Cho
  • Publication number: 20090176663
    Abstract: The present invention relates to compositions suitable for spotting compounds onto a substrate, and methods employing these compositions. The composition can include one or more organic anions of formula I: R(X)m(Y)n. In an embodiment of formula I, R is an organic moiety, X is an anionic moiety, and, Y is a neutral hydrophilic moiety. The composition can include one or more neutral hydrophilic polymers. In an embodiment, the neutral hydrophilic polymer can be represented by formula V: In Formula V, the group in parenthesis represents the polymer backbone, A can be absent or is a carbon or a heteroatom, and B is pendant neutral hydrophilic group. The present invention includes arrays, which can be made with or include compositions according to the present invention and can be made by using the method of the present invention.
    Type: Application
    Filed: December 8, 2008
    Publication date: July 9, 2009
    Applicant: SURMODICS, INC.
    Inventors: Gary Opperman, Asad Omar
  • Publication number: 20080182760
    Abstract: The present invention relates to a linker or population of linkers that include an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1. A linker-polynucleotide or a population of linker-polynucleotides of the invention may be constituted by said linker or population of linkers and a target first strand polynucleotide bound to said linker. The invention also encompasses a method of preparing said linker or population of linkers and a method of preparing a linker-polynucleotide using said linker or population of linkers. The linkers or polynucleotide-linkers of the invention can be used in a method of preparing a cDNA library.
    Type: Application
    Filed: July 5, 2007
    Publication date: July 31, 2008
    Inventor: Yoshihide HAYASHIZAKI
  • Publication number: 20080176761
    Abstract: Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.
    Type: Application
    Filed: November 21, 2007
    Publication date: July 24, 2008
    Inventors: Steven M. Menchen, Christina E. Inman, Meng Taing, Khai Luong, Handong Li