Abstract: A process of reducing malodors on fabrics includes combining fabrics having at least one source of malodor with a wash liquor having metal ions. The wash liquor is prepared by diluting a laundry detergent composition in water by a factor of between 100-fold and 3000-fold. The process further includes washing the fabrics in the wash liquor using an automatic wash operation, a manual wash operation or a mixture thereof; separating the fabrics and the wash liquor from one another; and drying the fabrics. The laundry detergent composition includes from 0.001% to 5% of an alkylated phenol antioxidant, a hindered phenol antioxidant, or mixtures thereof, and at least one of said antioxidants has a log D value at pH 7 equal to or greater than 1.50.

Abstract: Methods of dishwashing to remove soils are disclosed, including a first detergent wash step, wherein the detergent is substantially-free of water conditioning agents including polycarboxylic acid polymers and phosphonates, followed by a second step of rinsing under high temperature with a water conditioning agent, namely polycarboxylic acid polymers. The methods result in little to no precipitation forming on the treated ware due to the treating of the hard water before it contacts the alkalinity source which prevents precipitation and/or flocculation from occurring.

Abstract: The invention includes ware detergent compositions which provides superior cleaning and removal of oily and fatty soils, without the production of excessive foam. According to the invention applicants have discovered that use of a quaternary cationic surfactant in combination with a nonionic low foaming surfactant can provide oily soil removal from ware that is superior to traditional warewash detergent formulations. Compositions for alkaline, preferably solid, warewash detergents are disclosed, as well as their use in dish machines and methods of manufacture.

Abstract: A hydrocolloid or aqueous solution comprising a poly alpha-1,3-glucan ether compound is disclosed having a viscosity of at least about 10 centipoise (cPs). The poly alpha-1,3-glucan ether compound in these compositions has a degree of substitution of about 0.05 to about 3.0. Also disclosed is a method for increasing the viscosity of a hydrocolloid or aqueous composition using a poly alpha-1,3-glucan ether compound.

Abstract: A fabric treatment composition is provided that includes at least one zeta potential modifier, a fluoropolymer and a hydrophobic agent with a melting point or glass transition temperature below 100° C., for imparting fabric protection benefits to a fabric, such as improved stain and soil resistance, oil repellency, water repellency, softness, wrinkle and damage resistance, and better hand feel. Fabric treatment compositions can be used as a pretreatment prior to washing, through soaking, or added to the treatment liquor, that is either the wash or rinse cycle of an automatic washing machine, to first provide and then maintain and refresh the fabric protection benefits imparted to the fabric, with the proviso that an intermediate rinsing step essentially devoid of added surfactant-is used after the washing cycle and prior to the fabric treatment step.

Abstract: A natural biotech washing agent for the environment, which is truly natural and environment friendly, for removing oil stains and dirt on a clothing object and degrading the oil and dirt at the same time without using any powered tools, comprising a composition by percentage weight containing 0.5% marine alkaloids, 6.5-9.5% surfactant and 2.5-3.5% enzymes as active ingredients. The biotech washing agent is truly meant for the environment in that the composition is natural, the cleaning process is natural and energy-saving, the cleaning effect is great, the end products after cleaning is natural and cause no harm to the environment, and no powered tools is required to achieve the cleaning effect. In addition, the wastewater after the washing process of the present invention can continue to provide a cleaning effect to the sewage and the wastewater.

Abstract: The invention provides a method of controlling biological fouling of water containing items. The method involves combining at least two reactants, a peroxygen source and an acyl group donor to form peracetic acid. Because the peracetic acid can be made in situ, the entire sum of its short lifespan can be used controlling biocidal fouling and storage and transport is simplified. The invention further facilitates the ease of controlling biocidal fouling by allowing the injection of the biocidal agent in liquid form, in a non-acidic environment, and without the need for any halogen. This allows the inventive biocide to be used with chemistries for which prior art biocides are inapplicable.

Abstract: A hydrolytic enzyme is stabilized in a liquid surfactant preparation by using a component that stabilizes the hydrolytic enzyme and includes a monosaccharide glycerate.

Abstract: The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.

Abstract: The present invention relates to methods for producing variants of a parent RP-II protease and the variants having altered properties as compared to the parent RP-II protease.

Abstract: The present invention provides novel serine proteases, novel genetic material encoding these enzymes, and proteolytic proteins obtained from Micrococcineae spp., including but not limited to Cellulomonas spp. and variant proteins developed therefrom. In particular, the present invention provides protease compositions obtained from a Cellulomonas spp, DNA encoding the protease, vectors comprising the DNA encoding the protease, host cells transformed with the vector DNA, and an enzyme produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising protease(s) obtained from a Micrococcineae spp., including but not limited to Cellulomonas spp. In alternative embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These mutant proteases also find use in numerous applications.

Abstract: Detergents and cleaning agents containing a protease having an amino acid sequence being at least 97.5% identical to the amino acid sequence given in SEQ ID NO. 3. The detergents and cleaning agents exhibit an excellent cleaning action on protease-sensitive soiling.

Abstract: This invention relates to consumer products comprising proteases, particularly cold water proteases and processes for making and using such products. Such consumer products provide improved cleaning, whiteness and/or freshness. Such proteases are derived from a parent enzyme, for example Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.

Abstract: A detergent composition comprising; a subtilisin variant having the amino acid sequence set forth in SEQ ID NO 1, and at least one additional ingredient selected from; i) bleaches selected from percarbonates, persulphates and organic peracids, ii) aminocarboxylates, or iii) sulphonated polymers, or iv) organophosphoric acids or salts thereof and mixtures thereof is provided. Also provided is a detergent composition comprising a subtilisin variant having the amino acid sequence set forth in SEQ ID NO 1, wherein, the detergent composition is at least partially enveloped in a water soluble or water dispersible package. The compositions exhibit good performance on proteinaceous stains, even when formulated at alkaline pHs. A method of removing proteinaceous stains from surfaces comprising such stains is also provided.

Abstract: The need for a bleaching composition providing improved removal of fatty stains and red food stains, particularly during low temperature washing, can be met by incorporating an aryliminium organic bleach catalyst, an activated peroxygen source, and a bleach activator, in specific levels.

Abstract: A process is provided to produce a concentrated aqueous peracid solution in situ using at least one enzyme having perhydrolase activity in the presence of hydrogen peroxide (at a concentration of at least 500 mM) under neutral to acidic reaction conditions from suitable carboxylic acid esters (including glycerides) and/or amides substrates. The concentrated peracid solution produced is sufficient for use in a variety of disinfection and/or bleaching applications.

Abstract: The present invention provides a liquid detergent composition comprising an effective amount of an enzyme, 0.001-3% wt of a perfume ingredient, and 0.005-2% wt of an anti-oxidant. It has been found that the storage stability of the enzyme—which is preferably a protease—can be drastically improved by the addition of the anti-oxidant.

Abstract: This invention relates to compositions comprising certain cellobiose dehydrogenase enzymes and processes for making and using such compositions, including the use of such compositions to clean and/or treat a situs.

Abstract: The invention provides an activation method of ?-amylase. ?-Amylase or particles containing the same are contacted with an oxidizing agent or particles containing the same. After contacting, the activated ?-amylase can be separated from the oxidizing agent. After contacting, ?-amylase and the oxidizing agent can be incorporated in the form of a mixture into a detergent base to give a detergent composition.

Abstract: The invention is related to mild pretreatment or preparation process as well as combined scoring and bleaching composition for cellulose-based textile material. The process comprises a sequential or simultaneous scouring step with an enzyme composition acting on the non-cellulosic material on outer layers of cellulosic fibers and a reducing bleaching step with a reducing bleaching agent. The reducing bleaching agent is preferably dithionite. In the process no adjustment of pH and temperature are required. The process is environmentally friendly because no causticizing and oxidizing agents are required to achieve the desired lightness and wettability. Due to the mild pretreatment conditions tensile strength of the fabric is better than that achieved with conventional pretreatments.

Abstract: A process is provided to produce a concentrated aqueous peracid solution in situ using at least one enzyme having perhydrolase activity in the presence of hydrogen peroxide (at a concentration of at least 500 mM) under neutral to acidic reaction conditions from suitable carboxylic acid esters (including glycerides) and/or amides substrates. The concentrated peracid solution produced is sufficient for use in a variety of disinfection and/or bleaching applications.

Abstract: The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba. The invention also relates to DNA encoding such proteases and the expression thereof in a host cell, animal and plant cells, as well as to the use thereof in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).

Abstract: A method is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted in situ with an inorganic peroxide such as hydrogen peroxide in the presence of a perhydrolase catalyst derived from a Lactobacillus sp. to produce peroxycarboxylic acids.

Abstract: A process is provided to produce a concentrated aqueous peracid solution in situ using at least one enzyme having perhydrolase activity in the presence of hydrogen peroxide (at a concentration of at least 500 mM) under neutral to acidic reaction conditions from suitable carboxylic acid esters (including glycerides) and/or amides substrates. The concentrated peracid solution produced is sufficient for use in a variety of disinfection and/or bleaching applications.

Abstract: Laundry product compositions containing a stable mixture of at least one water insoluble fabric care benefit agent and at least one delivery enhancing agent that is preferably a silicone derivative. Detergent laundry product compositions containing from about 1% to about 80%, by weight of the composition, of a detersive surfactant that is an anionic surfactant, cationic surfactant, nonionic surfactant, amphoteric surfactant, zwitterionic surfactant, or a mixture thereof; from about 0.1% to about 10%, by weight of the composition, of a water insoluble fabric care benefit agent; from about 0.01% to about 5%, by weight of the composition, of a delivery enhancing agent; and wherein the ratio of the delivery enhancing agent to the fabric care benefit agent is from about 1:50 to about 1:1.

Abstract: Laundry product compositions containing a stable mixture of at least one water insoluble fabric care benefit agent and at least one delivery enhancing agent that is preferably a dispersible polyolefin, polymer latex, or mixture thereof. Detergent laundry product compositions containing from about 1% to about 80%, by weight of the composition, of a detersive surfactant that is an anionic surfactant, cationic surfactant, nonionic surfactant, amphoteric surfactant, zwitterionic surfactant, or a mixture thereof; from about 0.1% to about 10%, by weight of the composition, of a water insoluble fabric care benefit agent; from about 0.01% to about 5%, by weight of the composition, of a delivery enhancing agent; and wherein the ratio of the delivery enhancing agent to the fabric care benefit agent is from about 1:50 to about 1:1.

Abstract: Compositions of the invention include: (a) a solvent having a hydrophilic-lipophilic balance (HLB) value of 5 to 7; and (b) a solubilizing agent having the formula: where R is aryl, cycloaryl, heteroaryl or hetercyclyl; R1 is a bond, (C1-C7)alkyl, substituted (C1-C7)alkyl, (C1-C7)alkoxy, substituted (C1-C7)alkoxy, (C2-C7)alkenyl or substituted (C2-C7)alkenyl; and Z+ is hydrogen, Na, K, NR?4 where R? is defined herein. Further compositions include: (a) a solvent having a (HLB) value of 5 to 7; and (b) a solubilizing agent having the formula: where R2 is a bond, (C1-C7)alkyl, substituted (C1-C7)alkyl, (C1-C7)alkoxy, substituted (C1-C7)alkoxy, (C2-C7)alkenyl or substituted (C2-C7)alkenyl; and Z+ is hydrogen, Na, K, NR?4 where R? is defined herein. The compositions are useful in providing stable single phase aqueous solutions when combined with water.

Abstract: A germicide and disinfectant composition contains detergent and at least two enzymes adapted to cause lysis in glycoprotein in infectious agents. These germicidal and disinfectant compositions are used in conjunction with prepackaged wipes and kits that allows the user to remove liquid or dried contaminated material without coming into direct contact with the contaminated material.

Abstract: Cleaning compositions, including laundry, dishwashing, hard surface cleaner, oral/dental cleaning compositions, comprising an oxidoreductase with an &agr;/&bgr;-hydrolase fold and a catalytic triad consisting of the amino acid residues serine, histidine and aspartic acid, which provide effective and efficient cleaning of colored and/or everyday body stains and/or soils and the sanitization of treated surface.

Abstract: Detergent compositions comprise a water-soluble builder, an enzyme and a polymeric dye transfer inhibiting agent, wherein a means is provided for delaying the release of the enzyme relative to the release of the water-soluble builder to the wash solution. The time to achieve a concentration that is 50% of the ultimate concentration of the water-soluble builder is less than 60 seconds, and the time to achieve a concentration that is 50% of the ultimate concentration of the enzyme is more than 90 seconds.

Abstract: Enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.

Abstract: Enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in any detergent in comparison to their wild type parent enzymes.

Abstract: The present invention relates to a method for fabric treatment. More specifically the invention relates to a convenient to carry fabric treatment applicator comprising a heat activatable compound such as a peroxide bleach, which can be advantageously used on clothes while they are worn. Claimed and described is a method for the application of a fabric treatment composition comprising a heat activatable compound such as a peroxide bleach onto a fabric whereby the temperature of the composition is raised with regard to the storage temperature. Further claimed is a fabric treatment applicator comprising a heat activatable compound and a flow interruption means, preferably a valve, to interrupt or allow the flow of the fabric treatment composition.

Abstract: An aqueous liquid cleaning composition having a pH of at least 7, preferably from 7 to 11, more preferably from 7 to 10 and comprising from 1% to 90% by weight of surfactant, a proteolytic enzyme and a primary stabiliser therefor, the composition further comprising an organic substance which forms a complex with a transition metal, the complex being capable of catalysing bleaching of a substrate by atmospheric oxygen.

Abstract: Cleaning compositions, including laundry, dishwashing, hard surface and/or oral/dental compositions comprising a cytochrome, particularly cytochrome P450. Said compositions provide effective and efficient cleaning of colored and/or everyday body stains and/or soils and provide sanitization of the treated surfaces. When formulated into a laundry detergent composition, the present invention provides fabric realistic item cleaning and enhanced whitening.

Abstract: The present invention relates to laundry detergent compositions, comprising a saccharide gum degrading enzyme, providing excellent cleaning performance, especially food stain/soil removal, dingy cleaning and whiteness benefits.

Abstract: There is provided an antibody granule, consisting essentially of one or more antibodies, or fragments derived thereof, granulated with an alkali metal salt. Also provided is a process for preparing said antibody granules. The granules can be used in an enzymatic stain bleaching or anti dye-transfer composition.

Abstract: The present invention relates to subtilase variants having a reduced tendency towards inhibition by substances present in eggs, such as trypsin inhibitor type IV-0. In particular, the variants comprise at least one additional amino acid residue between positions 42-43, 51-56, 155-161, 187-190, 216-217, 217-218 or 218-219 (in BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash composition, including automatic dishwash compositions. Also, isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, cleaning and detergent compositions comprising the variants are disclosed.

Abstract: The present invention is a blood and organic stain remover which includes in a first embodiment a protease, an amylase, an enzyme stabilizing system such as a source of calcium, an alcohol, and an alkanolamine, a nonionic detergent, and water. In a second embodiment, the blood and organic stain remover includes a protease, an amylase, an enzyme stabilizing system, a nonionic detergent, an anionic detergent, and water. In the second embodiment the enzyme stabilizing system preferably includes a petroleum distillate. A system of using the aqueous blood and organic stain remover composition in a prepackaged wipe which permits the user to remove blood and organic spots or to pre-spot blood and organic stains prior to washing without the need for direct skin contact with the spot is also provided.

Abstract: The present invention provides a product and method for cleaning or laundering or treatment of fabrics or surfaces, with a liquid cleaning, laundering or treating composition having improved enzyme stability in the presence of an oxidant comprising a) at least one cross-linked hydrolase crystal, or mixture of hydrolase crystals; b) an oxidant, or source of hydrogen peroxide; and c) at least one cleaning, laundering or treating additive, other than an oxidant, wherein the improved stability is due to one or more of the following: (i) where c) includes a surfactant, maintaining the upper limit thereof to no greater than 10% by weight of the composition; (ii) maintaining the pH of the liquid composition between 5 and 8; and (iii) where c) includes surfactants, no more than 20% of the total surfactants is anionic.

Abstract: There is provided a process for enhancing the activity of a phenol oxidizing enzyme, comprising adding to the enzyme, as an enhancer for the activity of said enzyme, one or more compounds having the having the formula: wherein Z1 and Z2 are electron withdrawing groups, independently selected from the group consisting of optionally substituted alkyl/(hetero)aryl- -sulfone, -sulfoxide, -sulfonate, -carbonyl, -oxalyl, -amidoxalyl, -hydrazidoxalyl, -carboxyl and esters and salts thereof, -amidyl, -hydrazidyl, nitrile. The process is especially useful for removing colored stains from fabrics in a washing process.

Abstract: The present invention relates to cleaning compositions, including laundry, fabric care, dishwashing, hard surface cleaner, oral/dental cleaning compositions, comprising a polyphenol/heterocyclic substrate based oxygenase, which provide effective and efficient cleaning of coloured and everyday body stains and/or soils and provides sanitisation of the treated surfaces. Furthermore, the cleaning compositions of the present invention provide fabric realistic items cleaning and whitening performance while providing excellent fabric colour safety when formulated as a laundry detergent composition.

Abstract: There is provided a bleaching detergent composition comprising one or more surfactants, a phenol oxidizing enzyme and a compound which acts as enhancer for the phenol oxidizing enzyme, said composition having an Enhancer Index as herein defined of at least 12 and prefereably in the range of 15-50.

Abstract: The present invention provides a detergent composition which is excellent in enzyme stability and exhibits excellent detergency particularly to protein-related dirt of socks and other items even under laundering conditions at a lower temperature. That is, the present invention provides a detergent composition comprising specific proportions of (a) an anionic surfactant, (b) a chlorine scavenger, (c) a protease whose &agr;-keratin-hydrolyzing activity at 10° C. is not less than 0.09×10−3 &mgr;g/mPU·min and (d) a protease whose &agr;-keratin-hydrolyzing activity at 10° C. is less than 0.09×10−3 &mgr;g/mPU·min.

Abstract: The present relates to cleaning compositions comprising a protease enzyme which is a carbonyl variant having an amino acid sequence not found in nature, which is derived by replacement of a plurality of amino acid residues of a precursor carbonyl ydrolase with different amino acids.

Abstract: The present invention relates to detergent compositions, including laundry, dishwashing, and/or hard surface cleaner compositions, comprising a maltogenic alpha-amylase enzyme and a detergent ingredient selected from the group consisting of a nonionic surfactant, a protease, a bleaching agent and/or mixtures thereof. Such compositions provide excellent removal of starch-containing stains and soils, and when formulated as laundry compositions, excellent whiteness maintenance and dingy cleaning.

Abstract: There is provided a method of delivering a benefit agent to fabric for exerting a pre-determined activity, wherein the fabric is pre-treated with a multi-specific binding molecule which has a high binding affinity to said fabric through one specificity and is capable of binding to said benefit agent through another specificity, followed by contacting said pre-treated fabric with said benefit agent, to enhance said pre-determined activity to said fabric. Preferably, the binding molecule is an antibody or fragment thereof, or a fusion protein comprising a cellulose binding domain and a domain having a high binding affinity to another ligand which is directed to said benefit agent. The method is useful for example for stain removal, perfume delivery, and treating collars and cuffs for wear.

Abstract: There is provided a bleaching enzyme capable of generating a bleaching chemical and having a high binding affinity for non-colored compounds present in stains on fabrics, said non-colored compounds having a molecular weight of at least 100, preferably of at least 1,000 and more preferably of at least 5,000. Furthermore, there is provided an enzymatic bleaching composition comprising said bleaching enzyme and a surfactant and a process for bleaching stains present of fabrics.

Abstract: Detergents and cleaners having improved performance against protein-containing soils, containing a protease of the subtilisin type, in which—in at least one of positions 3, 4, 99, 188, 193, 199 and 211 (BLAP counting)—the amino acid present in that position in the wild-type protease is replaced by another amino acid, and coated particulate alkali metal percarbonate.

Abstract: There is provided a method of delivering a benefit agent to a whereby a benefit agent is first loaded to a surface and subsequently unloaded and transferred and delivered to a second surface. More in particular, the benefit agent is first loaded onto a garment during a laundering process, and subsequently delivered to another surface. The benefit agents can be fragrance agents, perfumes, color enhancers, fabric softening agents, polymeric lubricants, photoprotective agents, latexes, resins, dye fixative agents, encapsulated materials, antioxidants, insecticides, soil repelling agents, soil release agents, and cellulose fibers.