Abstract: The present invention relates to an article comprising: (a) a liquid composition comprising: (i) enzyme; and (ii) from 0% to 10% (by weight of said liquid composition) free water, preferably 0% to 5% free water; and (iii) carboxylic acid comprising 5 carbon atoms or less, and 1 or 2 carboxy groups; and (iv) chelating agent; and (v) enzyme stabilizing metal ion system consisting of calcium ions and magnesium ions, present in a weight ratio of calcium ion to magnesium ion of from 1:1 to 4:1; and (vi) from 0% to 0.2% (by weight of said liquid composition) source of borate ions; and (b) a water-soluble polymeric material that is capable of being cross-linked by borate ions, preferably a water-soluble polymeric material comprising poly-vinyl alcohol.

Abstract: Genes encoding novel cellulases, and a gene encoding a protein that facilitates the action of such novel cellulases, the novel cellulases and a protein that facilitates the action of such cellulases, and enzyme preparations containing such proteins are described. The native hosts and the culture medium of said hosts containing said novel cellulases are also disclosed. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.

Abstract: A bleaching composition is presented having a) an enzyme which produces hydrogen peroxide from atmospheric oxygen, b) a substrate for said enzyme, and c) a transition metal compound, where the enzyme is covalently bound to the transition metal compound. The bleaching composition is useful in disinfectants and laundry detergents as a bleaching component and for inhibiting the transfer of dyes.

Abstract: The present invention relates to variant EGIII cellulases that have improved stability and/or performance. The variant cellulases have replacements at sensitive residues to improve stability and/or performance.

Abstract: The present invention provides detergent compositions comprising variants of precursor phenol oxidizing enzymes, said precursor being derived from a Stachybotrys species. The precursor enzymes can be, for example, naturally-occurring or recombinant phenol oxidizing enzymes. The variant enzymes, in general, are obtained by modification of a precursor DNA sequence encoding the naturally-occurring or recombinant phenol oxidizing enzyme to generate the substitution of one or more amino acids in the encoded amino acid sequence. Variant enzymes are disclosed having properties which differ from those of their respective precursor enzymes, e.g., altered pH optima and/or phenol oxidizing activity and/or altered stability. The variant enzymes of the present invention are especially useful in detergent formulations, for example, to modify (bleach) the color associated with colored compounds.

Abstract: The present invention solves the problem of soil and dirt becoming entrained in cellulosic material loosened and removed from fabric during washing wherein said dirt and soil is entrapped by the cellulosic material and re-deposited onto the fabric surface.

Abstract: The present invention relates to Subtilisin DY variants having a modified amino acid sequence of wild-type Subtilisin DY amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type Subtilisin DY (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the Subtilisin DY variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type Subtilisin DY. The present invention also relates to DNA sequences encoding such Subtilisin DY variants. The present invention also relates to compositions comprising such Subtilisin DY variants for cleaning a variety of surfaces.

Abstract: The present invention relates to a composition containing MAP zeolite, ethoxylated nonionic surfactant and an alkali metal carbonate.

Abstract: This invention relates to an enzyme containing granular composition comprising:

Abstract: Improved, non-chlorinated systems and methods for removing soils from hard surfaces are provided wherein such surfaces are initially contacted with a first aqueous use dispersion including a protease enzyme, followed by contact the surface with a second aqueous acidic use dispersion. The enzyme dispersion preferably includes a polyol such as propylene glycol and in use is heated to a temperature up to about 120° C. The acid dispersion may include a surfactant and is also used in a heated condition. The two-component systems of the invention can be used for CIP treatment of dairy or other equipment in lieu chlorinated cleaning agents.

Abstract: There is provided an antibody granule, consisting essentially of one or more antibodies, or fragments derived thereof, granulated with an alkali metal salt. Also provided is a process for preparing said antibody granules. The granules can be used in an enzymatic stain bleaching or anti dye-transfer composition.

Abstract: The present invention concerns an enzyme containing cleaning particle having a size of more than 10.5 mm in its longest dimension, wherein the non-enzyme components of the particle have a detergency of less than 4.

Abstract: Detergent composition comprising fusion protein comprising a cellulose binding domain and a domain having a high binding affinity for another ligand. Preferably, the domain having a high binding affinity is directed at a Benefit Agent.

Abstract: Modified enzymes which comprise a catalytically active amino acid sequence of an enzyme, linked via a non-mino acid linking region to an amino acid sequence comprising a Cellulose Binding Domain. The present invention further relates to laundry detergent and/or fabric care compositions comprising such modified enzymes. These compositions provide a higher effective concentration of the enzyme at its substrate location and therefore, improved enzymatic benefits.

Abstract: The present invention relates to laundry and/or fabric and/or color care compositions; more particularly, to laundry and/or fabric and/or color care compositions containing xyloglucan endotransglycosylase enzyme (XET) in combination with a polysaccharide and/or oligosaccharide, preferably a xyloglucan polymer, that provide improved anti-wrinkle and/or shape retention and/or anti-shrinkage and/or tensile strength and/or color appearance and/or anti-bobbling and/or better static control, fabric softness, anti-wear properties and benefits, while at the same time providing improved cleaning benefits, over laundry and/or fabric and/or color care compositions without such combination of XET and polysaccharide and/or oligosaccharide.

Abstract: The present invention relates to a composition containing a MAP zeolite, ethoxylated nonionic surfactant and an alkali metal carbonate.

Abstract: A modified protein which comprises a catalytically active amino acid sequence of an antimicrobial enzyme and/or an amino acid sequence of an antimicrobial peptide linked to an amino acid sequence comprising a cellulose binding domain (CBD), and laundry detergents and/or fabric care compositions comprising such modified protein for improved sanitization benefits, are provided by the present invention.

Abstract: The present invention relates to a composition containing zeolite, ethoxylated nonionic surfactant and an alkali metal carbonate.

Abstract: The present invention provides a particle containing a peroxide-sensitive component (such as an enzyme; e.g., a hydrolase) and an added catalase component. The particle can be included in a detergent composition with peroxygen bleach.

Abstract: The present invention relates to polypeptides with reduced immune response including reduced allergenicity having one or more amino acid residues being substituted with other amino acid residues and/or having coupled one or more polymeric molecules in the vicinity of the polypeptides metal binding site, a method for preparing modified polypeptides of the invention, the use of the polypeptide for reducing the immunogenicity and allergenicity and compositions comprising the polypeptide.

Abstract: A detergent composition comprising a protease that is screened by a process comprising the steps of measuring the Primary Cleavage Rate and measuring the Second Cleavage Rate. The ratio of the Primary Cleavage Ratio to the Second Cleavage Rate of the protease is calculated. Proteases are selected that have a Primary Cleavage Rate higher than 1000 &mgr;g/min/&mgr;g and a ratio of the Primary Cleavage Rate to the Secondary Cleavage Rate lower than 150:1.

Abstract: There is provided an enzymatic detergent composition which comprises: (a) surfactant; and (b) 10-20,000 LU per gram of the detergent composition of a lipolytic enzyme obtainable from Humicola lanuginosa, Pseudomonas pseudoalcaligenes, Rhizomucor miehei and (c) a non-cross-bridged polydentate N-donor ligand capable of forming a complex with a transition metal, wherein said complex is capable of catalysing the bleaching of stains on fabrics by means of atmospheric oxygen.

Abstract: Haloperoxidases are capable of exhibiting excellent oxidizing, bleaching, and antimicrobial activity, when combined with carboxylic acids. Accordingly, the invention provides an enzymatic composition comprising a haloperoxidase, a source of hydrogen peroxide, and a source of a carboxylic acid.

Abstract: The present invention relates to subtilisin Carlsberg variants having a modified amino acid sequence of wild-type subtilisin Carlsberg amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type subtilisin Carlsberg (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the subtilisin Carlsberg variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type subtilisin Carlsberg. The present invention also relates to the genes encoding such subtilisin Carlsberg variants. The present invention also relates to compositions comprising such subtilisin Carlsberg variants for cleaning a variety of surfaces.

Abstract: The present invention relates to Proteinase K variants having a modified amino acid sequence of wild-type Proteinase K amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type Proteinase K (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the Proteinase K variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type Proteinase K. The present invention also relates to DNA sequences encoding such Proteinase K variants. The present invention also relates to compositions comprising such Proteinase K variants for cleaning a variety of surfaces.

Abstract: A method for treating cellulosic materials is disclosed which comprises contacting the cellulosic material with a cellulase obtainable from Thermomonospora fusca corresponding to E5 or a derivative thereof. Particularly preferred methods comprise stonewashing and detergent cleaning of cotton fabrics, the production of paper products, as an additive to animal feed and in the production of food, starch, ethanol and sugar.

Abstract: The present invention relates to cleaning compositions comprising a protease variant.

Abstract: Laundry detergent compositions containing one or more cellulases having a ratio of tensile strength loss to antipilling properties of less than 1. The cellulases may be obtained from CBS 9.93 or CBS 670.93.

Abstract: A composition for simultaneous cleaning and decontaminating a device. The composition is a per-compound oxidant in an amount effective for decontaminating the device and an enzyme in an amount effective for cleaning the device. The device may be a medical device such as an endoscope or kidney dialyzer and a plurality of devices can be cleaned using the same composition. The composition may additionally contain a corrosion inhibitor in an amount effective to prevent corrosion of a metal, a chelator, a buffer, a dye and combinations thereof.

Abstract: The present invention relates to cleaning compositions a mannanase and a carbohydrase selected from cellulases, amylases, pectin degrading enzymes and/or xyloglucanases. These compositions provide superior cleaning performance, i.e. superior stain removal, dingy cleaning and whiteness maintenance.

Abstract: A detergent composition comprises a zeolite builder having a particle size, expressed as a d50 value, of less than 1.0 micrometers, an amylase enzyme, and an alkoxylated nonionic surfactant having a hydrophilic lipophilic balance value of less than 9.5 selected from the group consisting of alkoxylated adducts of fatty alcohols containing an average of less than 5 alkylene oxide groups per molecule. The detergent composition has been found to reduce white residue formation on fabrics washed with detergent containing small particle size zeolite.

Abstract: The present invention relates to subtilisin BPN′ variants comprising at least one or more amino acid positions having a different amino acid than that occurring in wild-type subtilisin BPN′ (i.e., substitution) at specifically identified positions, whereby the BPN′ variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to wild-type subtilisin BPN′. The present invention further relates to various cleaning compositions comprising such BPN′ variants.

Abstract: The present invention relates to subtilisin BPN′ variants having a modified amino acid sequence of wild-type BPN′ amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region and a fifth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type subtilisin BPN′ (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the BPN′ variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type subtilisin BPN′. The present invention also relates to the genes encoding such subtilisin BPN′ variants. The present invention also relates to compositions comprising such subtilisin BPN′ variants for cleaning a variety of surfaces.

Abstract: The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.

Abstract: The present invention relates to a particle comprising an active dispersed in a visco-elastic liquid matrix having a &eegr;′ and a &eegr;″ between 103 to 1014 Pa measured in a cone-and-plate rheometer at 25° C. and a sinusoidal frequencies &ohgr; of 1 Hz.

Abstract: A particle for detersive enzymes is disclosed by way of the present invention. The particle comprises a composite particle suitable for incorporation into a detergent composition comprising an enzyme containing core material and a water soluble carboxylate barrier layer coated on the enzyme containing core material. The preferred enzymes are protease enzymes. Automatic dishwashing compositions employing the particle are also disclosed.

Abstract: The present invention provides thermophilic alkaliphilic bacteria designated Thermopallium natronophilum and thermophilic alkaliphilic polypeptides obtainable therefrom. It also provides compositions, particularly detergent compositions comprising the polypeptides.

Abstract: The present invention provides an agent for contact lenses, which contains an oxo-acid compound and/or a polyacid compound. The agent for contact lenses of the present invention can remove protein as, for example, a protein remover, irrespective of the kind of contact lens and without using a special cleaning device. In addition, washing with water after protein removal is not necessary.

Abstract: The present invention provides an improved coating material for use with particles, such as enzyme granules, and the like. In particular, the present invention provides a modified PVA, as well as particles or granules that include such coating. The PVA is modified by substituting hydrophilic moieties for the hydroxyl or alcohol groups of the PVA. Substitution may be achieved with hydrophilic acids, amines, thiols, or combinations thereof.

Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having proteolytic activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.

Abstract: A selective targeting method is disclosed comprising contacting a library of ligands, particularly a peptide library, with an anti-target to allow the ligands to bind to the anti-target; separating the non-binding ligands from the anti-target bound ligands, contacting the non-binding anti-target ligands with a target allowing the unbound ligands to bind with the target to form a target-bound ligand complex; separating the target-bound ligand complex from ligands which do not bind to the target, and identifying the target-bound ligands on the target-bound ligand complex wherein the target-bound ligands have a KD in the range of about 10−7 to 10−10 M. Additionally claimed are the ligands identified according to the method.

Abstract: The present invention relates to variants of a parent &agr;-amylase, which parent &agr;-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No.

Abstract: The present invention relates to detergent compositions comprising a mannasase enzyme and a bleach system preferably comprising a source of hydrogen peroxide and optionally, but preferably, a hydrophobic bleach activator for superior cleaning, stain removal and/or whiteness performance.

Abstract: A process for encapsulating a benefit agent and/or colorant for personal care or detergent compositions, the process comprising immersing an emulsion or a dispersion containing the benefit agent and/or colorant into an aqueous curing solution containing a high HLB surfactant and/or a super-wetting agent. The preferred process further includes increasing the density of the droplets, to allow for easier penetration into the curing solution.

Abstract: A detergent composition comprising a &bgr;-glucanase is presented. The &bgr;-glucanase improves the cleaning performance of the detergent, especially with respect to polysaccharide soils.

Abstract: A process for preparing a multi-phase detergent tablet comprising the steps of: a) forming a tablet body by compressing a granular detergent mixture, said tablet body having a first surface, said first surface having at least one mold therein and said granular detergent mixture comprises at least one detergent active agent; b) providing a gelatinous mixture under constant agitation, and delivering said gelatinous mixture to said at least one mold in said tablet body to form a gelatinous portion, said gelatinous mixture comprises at least one detergent active agent; and c) hardening or curing said gelatinous portion to form a multi-phase detergent tablet.

Abstract: The present invention provides low-density compositions, as well as particulates formed, at least in part, from such compositions. Preferred low-density materials include, for example, hollowspheres, low-density minerals, and low-density wood materials (e.g., sawdust). The low-density compositions of the invention can be formed as particulates, or cores, suitable for use in forming enzyme granules, e.g., marums, layered granules, prills, drum granules, agglomerated granules, or the like. Granules are disclosed having advantageous properties, e.g., low dusting, storage stable, fast enzyme-release profile, low true density, etc. The granules of the invention are especially useful, for example, in liquid detergents and cleaners, such as predominantly aqueous, liquid laundry detergents. In one embodiment, granules are provided having a true, or volumetric, density within a range of from about 0.95 to about 1.4 g/cm3.

Abstract: An aqueous hard surface cleaning composition contains anionic and nonionic surfactants, an enzyme mixture such as lipase/alpha-amylase for breaking down organic compounds; an activator for rendering the enzyme more active; and water. A progressively active composition also includes nonpathogenic bacteria such as a culture of Bacillus subtilis and Bacillus amyloliquefaciens for degrading and assimilating organic compounds.

Abstract: The present invention provides a product and method for cleaning or laundering or treatment of fabrics or surfaces, with a liquid cleaning, laundering or treating composition having improved enzyme stability in the presence of an oxidant comprising a) at least one cross-linked hydrolase crystal, or mixture of hydrolase crystals; b) an oxidant, or source of hydrogen peroxide; and c) at least one cleaning, laundering or treating additive, other than an oxidant, wherein the improved stability is due to one or more of the following: (i) where c) includes a surfactant, maintaining the upper limit thereof to no greater than 10% by weight of the composition; (ii) maintaining the pH of the liquid composition between 5 and 8; and (iii) where c) includes surfactants, no more than 20% of the total surfactants is anionic.

Abstract: The present invention relates to a modified enzyme which comprises a catalytically active amino acid sequence of a transferase linked to an amino acid sequence comprising a Cellulose Binding Domain (CBD). The present invention further relates to laundry detergent and/or fabric care compositions comprising such modified enzyme, for improved fabric care and cleaning benefits.