Insect Destroying Or Inhibiting Patents (Class 514/4.5)
-
Publication number: 20120255065Abstract: Provided are pesticidal, particularly insecticidal, proteins which resemble S-layer proteins, as well as variants or mutants thereof, and DNAs encoding them. Further provided are methods and means for using said DNA or protein for controlling pests, particularly plant insect pests.Type: ApplicationFiled: April 17, 2012Publication date: October 4, 2012Applicant: Universidad Nacional Autonoma de MexicoInventors: Mario SOBERON-CHAVEZ, Alejandra Bravo-De-La-Parra
-
Patent number: 8269069Abstract: The present invention includes domain I modifications that improve various attributes of various Coleopteran-active Cry proteins. These attributes can include improved target pest spectrum, potency, and insect resistance management. The subject modifications can affect protoxin activation and the efficiency of pore formation, which can lead to enhanced insect intoxication.Type: GrantFiled: July 30, 2009Date of Patent: September 18, 2012Assignee: Dow AgroSciences, LLCInventors: Kenneth Narva, Ignacio M. Larrinua, Timothy D. Hey
-
Publication number: 20120233726Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: March 7, 2012Publication date: September 13, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDRE R. ABAD, HUA DONG, SUE B. LO, XIAOMEI SHI, THOMAS C. WOLFE
-
Publication number: 20120220521Abstract: This invention relates in part to modifying BtBooster (BtB) peptides, in part to increase their stability in insect midgut digestive juices. Some preferred embodiments of BtB have removed proteinase cleavage sites resulting in increased stability of the modified BtB in the insect gut, while retaining the ability to enhance B.t. proteins for improved insect control. In some preferred embodiments, the protease-stable BtB is used in combination with B.t. spores and/or crystals comprising a Cry protein. Also reported herein is the significant and increased enhancement of Bt toxins against relatively Bt-tolerant insects (Helicoverpa zea, Spodoptera exigua and Agrotis ipsilon), when used with BtBs. We also describe increased toxin enhancement with cadherin fragments that are stabilized against over-digestion by insect midgut proteinases. We also report enhancement of Bt Cry1F toxin by cadherin fragments.Type: ApplicationFiled: August 11, 2008Publication date: August 30, 2012Inventors: Mohd Amir-Fursan Abdullah, Michael J. Adang
-
Publication number: 20120210464Abstract: Improved compositions and methods for controlling pests are disclosed. In particular, novel engineered Cry1Ba (eCry1Ba) proteins having improved toxicity to lepidopteran insect pests are provided. By substituting at least one amino acid in domain I of a Cry1Ba protein an engineered Cry1Ba protein having substantially altered insecticidal properties is designed. Further, a method of making the engineered Cry1Ba proteins and methods of using the ecry1Ba nucleic acid sequences, for example in transgenic plants to express eCry1B proteins to confer protection from insect damage are disclosed.Type: ApplicationFiled: September 27, 2010Publication date: August 16, 2012Applicant: SYNGENTA PARTICIPATIONS AGInventors: Yan Gao, Jared Conville, Jeng Shong Chen
-
Publication number: 20120210462Abstract: Traditionally, the primary method for impacting insect pest populations is the application of broad-spectrum chemical insecticides. However, there is increasing concern about the environmental hazards associated with the production and use of synthetic chemical pesticides. Thus, there is substantial interest in developing alternative pesticides, including biological control of insect pests of agricultural significance using a microbial agent or another species of insect. The present invention provides compositions and methods for such biological control. Modified Cry3 pesticidal polypeptides, polynucleotides encoding such polypeptides, and methods of use are disclosed. The modified polynucleotides provided herein can be used to transform organisms and cells of hosts comprising plant, insects, and microorganisms. The expression of modified polypeptides can provide the host with improved insecticidal activity against one or more insect pathogens.Type: ApplicationFiled: February 10, 2012Publication date: August 16, 2012Applicant: Pioneer Hi-Bred International, Inc.Inventors: Ericka Bermudez, Ruth Cong, Jingtong Hou, Takashi Yamamoto
-
Patent number: 8236757Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 11 and 13, or the nucleotide sequences set forth in SEQ ID NOS:1, 10 and 12, as well as variants and fragments thereof.Type: GrantFiled: January 21, 2010Date of Patent: August 7, 2012Assignee: Athenix CorpInventors: Nadine Carozzi, Tracy Hargiss, Michael G. Koziel, Nicholas B. Duck
-
Patent number: 8236336Abstract: An adjuvant for use with systemic herbicides, pesticides, insecticides, ovicides and fungicides and method of application on animals, birds, trees, plants, fruits and vegetables to enhance the action and effect of systemic herbicides, pesticides, insecticides, ovicides and fungicides with which the adjuvant is combined wherein the adjuvant comprises at least one surfactant and at least one high terpene containing natural oil.Type: GrantFiled: November 13, 2007Date of Patent: August 7, 2012Assignee: Oro Agri, Inc.Inventor: Erroll M. Pullen
-
Patent number: 8237021Abstract: The present invention relates to the field of plant pest control, particularly insect control. Provided are nucleotide sequences from Bacillus thuringiensis encoding insecticidal proteins. Further provided are methods and means for using said nucleotide sequence for controlling plant insect pests.Type: GrantFiled: June 25, 2010Date of Patent: August 7, 2012Assignee: Bayer CropScience N.V.Inventors: Greta Arnaut, Annemie Boets, Karel De Rudder, Stijn Vanneste, Jeroen Van Rie
-
Publication number: 20120192317Abstract: The invention provides methods for selecting nucleotide sequences that yield dsRNA-mediated gene suppression in a target organism and enable their uptake by the target organism. The invention further provides expression constructs that confer stabilized expression of such sequences in a transgenic host cell, and methods for their use. Also provided are organisms, cells and tissues prepared by a method of the invention.Type: ApplicationFiled: November 28, 2011Publication date: July 26, 2012Inventors: GREGORY R. HECK, Tichafa R. I. Munyikwa, Jean C. Goley, James K. Roberts, Scott C. Johnson, Ty T. Vaughn
-
Publication number: 20120192310Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: January 23, 2012Publication date: July 26, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
-
Publication number: 20120171183Abstract: This invention provides a composition comprising a biologically effective amount of a compound of Formula 1, an N-oxide or an agriculturally suitable salt thereof wherein R1, R2, R3, R4a, R4b and R5 are as defined in the disclosure, and an effective amount of at least one fungicide.Type: ApplicationFiled: March 6, 2012Publication date: July 5, 2012Applicant: E.I. DU PONT DE NEMOURS AND COMPANYInventors: George Philip Lahm, Thomas Paul Selby, Thomas Martin Stevenson
-
Publication number: 20120167259Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: December 22, 2011Publication date: June 28, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Lu Liu, Takashi Yamamoto, Yi Zheng
-
Publication number: 20120164205Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.Type: ApplicationFiled: September 6, 2011Publication date: June 28, 2012Inventors: James A. Baum, Larry A. Gilbertson, David K. Kovalic, Thomas J. LaRosa, Maolong Lu, Tichafa R. I. Munyikwa, James K. Roberts, Wei Wu, Bei Zhang
-
Publication number: 20120156262Abstract: Disclosed is an insecticidal suspension concentrate composition comprising by weight based on the total weight of the composition: (a) from about 0.3 to about 30% of 3-bromo-1-(3-chloro-2-pyridinyl)-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1H-pyrazole-5-carboxamide; (b) from about 5 to about 70% of a nonionic ethylene oxide-propylene oxide block copolymer component having a water solubility of at least about 5% by weight at 20° C., a hydrophilic-lipophilic balance value ranging from about 5 to about 18 and an average molecular weight ranging from about 900 to about 20000 daltons; and (c) from about 20 to about 95% of water. Also disclosed is a method for controlling an insect pest comprising diluting said suspension concentrate composition with water to form a diluted composition, and contacting the insect pest or its environment with an insecticidally effective amount of said diluted composition.Type: ApplicationFiled: September 3, 2010Publication date: June 21, 2012Inventors: Oiver Walter Gutsche, John Henry Green
-
Publication number: 20120156167Abstract: The isolation, cloning of an enhancin gene bel1 from Bacillus thuringiensis, and the use of an enhancin Bel1 in biological insecticides are disclosed in the present invention. A new enhancin gene bel1 is isolated from Bacillus thuringiensis subsp. kurstaki YBT-1520, and its encoded product is a new enhancin Bel1. The enhancin has very strong effect-enhancing activity on the insecticidal action of insecticidal crystal protein Cry1Ac in killing Lepidoptera insects. The insecticidal activity of genetically engineered Bacillus thuringiensis BMB0187 developed on the basis of this enhancin is 5.7 times as high as that of the initial strain.Type: ApplicationFiled: November 23, 2009Publication date: June 21, 2012Applicant: HUAZHONG AGRICULTURAL UNIVERSITYInventors: Ming Sun, Ziniu Yu, Shangling Fang, Donghai Peng, Lifang Ruan, Shouwen Chen, Chunping Luo
-
Publication number: 20120148497Abstract: Methods are provided for using field-derived colonies of insects that comprise field-evolved resistance to insecticidal toxins that are produced in transgenic plants. The methods find use in resistance management strategies for transgenic crop plants expressing insecticidal toxins.Type: ApplicationFiled: December 9, 2011Publication date: June 14, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventor: Analiza Alves
-
Patent number: 8188036Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: May 27, 2009Date of Patent: May 29, 2012Assignee: E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Nicholas B. Duck, Xiang Feng, Ronald D. Flannagan, Theodore W. Kahn, Lynne E. Sims
-
Publication number: 20120122680Abstract: Disclosed are compounds of Formula 1, N-oxides, and salts thereof, wherein X is O or S; Y is O or S; Z is a direct bond, O, S(O)n, NR6, C(R7)2O, OC(R7)2, C(?X1), C(?X1)E, EC(?X1), C(?NOR8) or C(?NN(R6)2); a is 0, 1, 2 or 3; and R1, R2, R3, R4, R5a, R5b, R6, R7, R8, X1 and E are as defined in the disclosure. Also disclosed are compositions containing the compounds of Formula 1 and methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a compound or a composition of the invention.Type: ApplicationFiled: August 3, 2010Publication date: May 17, 2012Applicant: E.I. Du Pont de Nemours and CompanyInventors: Caleb William Holyoke, JR., Wenming Zhang, kanu Maganbhai Patel, George Philip Lahm, My-Hanh Thi Tong
-
Publication number: 20120117690Abstract: The present invention provides insecticidal polypeptides related to shuffled Bacillus thuringiensis Cry1 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.Type: ApplicationFiled: January 24, 2012Publication date: May 10, 2012Applicant: Pioneer Hi-Bred International, Inc.Inventors: David Cerf, Ruth Cong, Michael Freeman, Kevin McBride, Takashi Yamamoto
-
Patent number: 8173590Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.Type: GrantFiled: February 15, 2008Date of Patent: May 8, 2012Assignee: Athenix Corp.Inventors: Nadine Carozzi, Tracy Hargiss, Michael G. Koziel, Nicholas B. Duck, Brian Carr
-
Patent number: 8173871Abstract: Provided are pesticidal, particularly insecticidal, proteins which resemble S- layer proteins, as well as variants or mutants thereof, and DNAs encoding them. Further provided are methods and means for using said DNA or protein for controlling pests, particularly plant insect pests.Type: GrantFiled: July 4, 2006Date of Patent: May 8, 2012Assignee: Universidad Nacional Autonoma de MexicoInventors: Mario Soberon-Chavez, Alejandra Bravo-De-La-Parra
-
Publication number: 20120088719Abstract: Provided is a bacterial strain which produces a family of mosquitocidal toxins, Xenorhabdus MT, on deposit with the American Type Culture Collection, PTA-6826, insecticidal compositions comprising the mosquitocidal toxin(s) produced by Xenorhabdus MT, a mosquitocidal toxin preparation prepared from spent culture medium, whole culture or cells or a mixture thereof, of Xenorhabdus MT and method of insect control, especially mosquito control. Also provided are microbial compounds (same as mosquitocidal toxins) compositions comprising them and use in formulating therapeutic and other antimicrobial compositions, and methods of use for inhibiting microbial growth and for treating infection.Type: ApplicationFiled: September 16, 2011Publication date: April 12, 2012Inventors: Jerald Coleman Ensign, Que Lan, David Dyer
-
Patent number: 8147856Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences.Type: GrantFiled: February 2, 2009Date of Patent: April 3, 2012Assignee: Athenix Corp.Inventors: Nadine Carozzi, Nicholas Duck, Theodore Kahn, Nalini Desai, Shruti Agarwal, Daniel John Tomso, Rong Guo, Julia Daum
-
Publication number: 20120066793Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.Type: ApplicationFiled: November 21, 2011Publication date: March 15, 2012Applicant: Athenix CorpInventors: Kimberly S. Sampson, Daniel John Tomso
-
Publication number: 20120065127Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: November 21, 2011Publication date: March 15, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
-
Patent number: 8133858Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3, 5, and 7, and the nucleotide sequences set forth in SEQ ID NO:1, 2, 4, and 6, as well as variants and fragments thereof.Type: GrantFiled: February 5, 2008Date of Patent: March 13, 2012Assignee: Athenix Corp.Inventors: Nadine Carozzi, Tracy Hargiss, Michael G. Koziel, Nicholas B. Duck, Brian Carr
-
Patent number: 8129594Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: June 3, 2009Date of Patent: March 6, 2012Assignee: Pioneer Hi-Bred International, Inc.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
-
Patent number: 8129593Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: April 3, 2009Date of Patent: March 6, 2012Assignee: Pioneer Hi-Bred International, Inc.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
-
Publication number: 20120047605Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: August 12, 2011Publication date: February 23, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
-
Publication number: 20120047607Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: August 17, 2011Publication date: February 23, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: André R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi
-
Publication number: 20120047606Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: August 15, 2011Publication date: February 23, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
-
Patent number: 8101408Abstract: The invention may be summarized as follows. The present invention provides insecticides comprised of naturally-occurring compounds, such as, for example, PA1b-related peptides and terpenoid saponins. The present invention also provides for identification and characterization of a synergistic insecticidal effect between peptides and saponins extracted from plants. Furthermore, simplified extraction procedures are provided that avoid the use of a chloroform defatting step or a column chromatography step.Type: GrantFiled: June 9, 2009Date of Patent: January 24, 2012Assignee: Her Majesty the Queen in right of Canada, as represented by the Minister of Agriculture and Agri-FoodInventors: Wesley G. Taylor, Paul G. Fields, Daniel H. Sutherland
-
Patent number: 8101568Abstract: The subject invention pertains to the use of peptide fragments of cadherins (including cadherin-like proteins). The subject invention includes a cell (and use thereof) comprising a polynucleotide that expresses the peptide fragment. The subject invention includes methods of feeding the peptides to insects. In preferred embodiments, the peptides are fed to target insects together with one or more insecticidal proteins, preferably (but not limited to) B.t. Cry proteins. When used in this manner, the peptide fragment can not only enhance the apparent toxin activity of the Cry protein against the insect species that was the source of the receptor but also against other insect species. Preferably, the cadherin is a Bacillus thuringiensis (B.t.) insecticidal crystal protein (Cry) toxin receptor. Preferably, the peptide fragment is a binding domain of the receptor. In some preferred embodiments, the peptide is the binding domain nearest to the membrane proximal ectodomain.Type: GrantFiled: June 27, 2008Date of Patent: January 24, 2012Assignee: University of Georgia Research Foundation, Inc.Inventors: Michael J. Adang, Gang Hua, Jian (John) Chen, Mohd Amir Fursan Abdullah
-
Patent number: 8101172Abstract: A Unique Solenopsis invicta virus (SINV 3) has been identified and its genome sequenced. Oligonucleotide primers have been developed using the isolated nucleic acid sequences of the SINV 3. The virus is used as a biocontrol agent for control of fire ants.Type: GrantFiled: February 18, 2009Date of Patent: January 24, 2012Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Steven Valles, Yoshifumi Hashimoto
-
Patent number: 8084418Abstract: The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin.Type: GrantFiled: February 16, 2009Date of Patent: December 27, 2011Assignee: Dow AgroSciences LLCInventors: Timothy D. Hey, Amanda D. Schleper, Scott A. Bevan, Scott B. Bintrim, Jon C. Michell, Ze Sheng Li, Weiting Ni, Baolong Zhu, Donald J. Merlo, Patricia C. Apel-Birkhold, Thomas Meade
-
Publication number: 20110289627Abstract: Methods for making a modified Cry3A toxin are disclosed. Such methods include the insertion of a protease recognition site that is recognized by a gut protease of a target insect, such as corn rootworm, into at least one position of a Cry3A toxin so that a modified Cry3A toxin is thus designed. The coding sequence of the modified Cry3A toxin may be transformed into a host cell and the host cell grown under conditions that allow the host cell to produce the modified Cry3A toxin. The host cell may be a plant cell and the plant may be comprised in a transgenic plant. Thus, the transgenic plant may be used to produce the modified Cry3A toxin.Type: ApplicationFiled: July 25, 2011Publication date: November 24, 2011Inventors: Eric Chen, Cheryl Stacy
-
Publication number: 20110283417Abstract: The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.Type: ApplicationFiled: June 29, 2011Publication date: November 17, 2011Inventors: JUDITH DONOVAN, WILLIAM DONOVAN, JAMES ENGLEMAN, THOMAS MALVAR, JOHN PITKIN
-
Patent number: 8057801Abstract: A novel galectin, a polynucleotide encoding the same, a vector and a transformant comprising the polynucleotide, an antibody against the galectin, and a screening method for screening a substance capable of modifying the galectin, are disclosed. According to the galectin, polynucleotide, or vector, it is possible, for example, to exterminate ticks, or to treat or prevent tick-borne infections such as rickettsiosis, filariasis, Q fever, African recurrent fever, or viral encephalitis.Type: GrantFiled: April 21, 2009Date of Patent: November 15, 2011Assignees: Meiji Seika Kaisya Ltd.Inventors: Kozo Fujisaki, Hideyuki Nagasawa, Ikuo Igarashi, Hiroshi Suzuki, Chihiro Sugimoto, Gakunan Gen, Kimie Kadota, Noboru Inoue, Naoaki Yokoyama, Naotoshi Tsuji
-
Publication number: 20110275557Abstract: This invention provides truncated Cry 35 proteins that surprisingly and unexpectedly have increased pesticidal activity as compared to full-length Cry 35 proteins. The subject invention also includes polynucleotides that encode these truncated proteins, transgenic plants comprising a truncated gene of the subject invention, and transgenic plants that produce these truncated proteins. This invention further provides methods of controlling plant pests, including rootworms, with these truncated proteins. The truncated Cry35 proteins of the subject invention are preferably used in combination with Cry34 proteins, which are known in the art. Various surprising advantages of the subject invention will be apparent in light of this disclosure.Type: ApplicationFiled: July 21, 2011Publication date: November 10, 2011Applicant: Dow AgroSciences LLCInventors: Yong Gao, Rod Aaron Herman, Deborah Ann Schwedler, Xioaping Xu, Andrew William Carr, George Erwin Schwab
-
Publication number: 20110263488Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.Type: ApplicationFiled: June 24, 2011Publication date: October 27, 2011Applicant: ATHENIX CORP.Inventors: Nadine Carozzi, Michael G. Koziel, Nicholas B. Duck, Nalini M. Desai, Rong Guo, Daniel John Tomso, Rebekah Deter, Tracy Hargiss
-
Publication number: 20110263487Abstract: The present invention relates to a plant flour material comprising a ground, dried plant material, wherein the plant material contains a recombinant protein comprises a bactericidal or an insecticidal protein toxin, or combinations thereof. The present invention further relates to a seed oil body composition comprising a seed oil body fusion, said oil body fusion comprising an oil body protein fused to a recombinant protein comprising a bactericidal or an insecticidal protein toxin. The present invention provides for a method of abating or controlling the population of mosquitoes comprising administering to a water source suspected of containing mosquito larvae an effective amount of seed oil body preparation comprising one or more Bti toxins, optionally in combination with one or more BtBs.Type: ApplicationFiled: December 22, 2008Publication date: October 27, 2011Applicant: University of Georgia Research Foundation, Inc.Inventor: Richard B. Meagher
-
Patent number: 8034997Abstract: The present invention provides nucleotide sequences encoding an insecticidal protein exhibiting lepidopteran inhibitory activity, as well as a novel insecticidal protein referred to herein as a Cry1A.105 insecticide, transgenic plants expressing the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.Type: GrantFiled: August 30, 2006Date of Patent: October 11, 2011Assignee: Monsanto Technology LLCInventors: Natalia N. Bogdanova, David R. Corbin, Thomas M. Malvar, Frederick J. Perlak, James K. Roberts, Charles P. Romano
-
Publication number: 20110237502Abstract: A family of insecticidal polypeptides expressed in the venom gland of spiders of the genera Atrax and Hadronyche have been described. Also included are polynucleotides and expression vectors encoding the polypeptides and insect viruses and cells expressing the polypeptides. Transgenic plants and insects expressing the insecticidal polypeptides are also described. The insecticidal polypeptides may be employed in methods and compositions for treating insects, insect larvae, and plants.Type: ApplicationFiled: January 17, 2011Publication date: September 29, 2011Inventors: Glenn F. King, Brianna Sollod McFarland, Graham Nicholson, Simon Gunning
-
Publication number: 20110239331Abstract: A series of potent and highly specific insecticidal toxins characterized by an amino acid sequences SEQ ID NO: 2-35.Type: ApplicationFiled: January 25, 2011Publication date: September 29, 2011Inventors: Glenn F. King, Brianna Sollod McFarland
-
AXMI221z, AXMI222z, AXMI223z, AXMI224z, AND AXMI225z DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE
Publication number: 20110203015Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-32, or the nucleotide sequence set forth in SEQ ID NO:1-20, as well as variants and fragments thereof.Type: ApplicationFiled: February 18, 2011Publication date: August 18, 2011Applicant: Athenix Corp.Inventors: Kimberly Sampson, Daniel Tomso -
Publication number: 20110201549Abstract: The subject invention relates in part to the discovery that BtBooster (BtB) peptides enhance Bt strain and Cry protein toxicity to the lesser mealworm. The subject invention also relates in part to the discovery that Bt tenebrionis producing Cry3Aa crystals and Bt japonensis BuiBui producing Cry8Ca crystals are insecticidal to A. diperinus larvae (darkling beetle or lesser mealworm). The subject invention also relates in part to the discovery that fragments from cadherins of the western corn rootworm and the yellow mealworm enhance the toxicities of Bt tenebrionis and Bt japonensis BuiBui toxicity to coleopteran larvae of the genus Alphitobius. In addition, the subject invention relates in part to the use of cadherin fragments to enhance the toxicities of Cry3Aa, Cry3Bb and Cry8Ca to coleopteran larvae of the genus Alphitobius. The subject invention also relates in part to screening Bt strains and Cry proteins for toxicity to larvae of darkling beetles or lesser mealworm, Alphitobius diaperinus.Type: ApplicationFiled: July 30, 2009Publication date: August 18, 2011Applicant: The University of Georgia Research Foundation, IncInventor: Michael J. Adang
-
Publication number: 20110203014Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-30, or the nucleotide sequence set forth in SEQ ID NO:1-12, as well as variants and fragments thereof.Type: ApplicationFiled: February 18, 2011Publication date: August 18, 2011Applicant: Athenix Corp.Inventors: Kimberly Sampson, Daniel Tomso
-
Publication number: 20110197314Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.Type: ApplicationFiled: February 15, 2011Publication date: August 11, 2011Applicant: Athenix Corp.Inventors: Kimberly S. Sampson, Daniel John Tomso
-
Publication number: 20110183896Abstract: The subject invention relates in part to the discovery that a fragment from a cadherin of the western corn rootworm enhances Cry3 toxicity to larvae of naturally susceptible species. The subject invention also relates in part to the discovery that a cadherin fragment from a beetle enhances Cry3Aa and Cry3Bb toxicity to coleopteran larvae, particularly those in the family Chrysomelidae. Such cadherin fragments are referred to as Bt Boosters (BtBs). The subject invention can be extended to the use of BtBs with other coleopteran-toxic Cry proteins for controlling a wide range of coleopterans.Type: ApplicationFiled: August 11, 2008Publication date: July 28, 2011Applicant: THE UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INCInventors: Michael J. Adang, Mohd Amir Fursan Abdullah