Separation, Purification Or Synthesis Patents (Class 530/364)
  • Patent number: 10525107
    Abstract: Methods and compositions for treating and/or preventing aging-related conditions are described. The compositions used in the methods include fractions derived from blood plasma with efficacy in treating and/or preventing aging-related conditions such as neurocognitive disorders.
    Type: Grant
    Filed: April 27, 2017
    Date of Patent: January 7, 2020
    Assignee: Alkahest, Inc.
    Inventors: David Bell, Ian Gallager, Steven P. Braithwaite, S. Sakura Minami, Vu Dang, Joe McCracken, Karoly Nikolich
  • Patent number: 10501513
    Abstract: The invention relates to compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues, receptor uptake and/or kinetics, cellular access by the compositions, engagement with translational machinery, mRNA half-life, translation efficiency, immune evasion, protein production capacity, secretion efficiency, accessibility to circulation, protein half-life and/or modulation of a cell's status, function, and/or activity.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: December 10, 2019
    Assignee: ModernaTX, Inc.
    Inventors: Antonin De Fougerolles, Justin Guild
  • Patent number: 10493167
    Abstract: The invention relates to compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues, receptor uptake and/or kinetics, cellular access by the compositions, engagement with translational machinery, mRNA half-life, translation efficiency, immune evasion, protein production capacity, secretion efficiency, accessibility to circulation, protein half-life and/or modulation of a cell's status, function, and/or activity.
    Type: Grant
    Filed: January 11, 2017
    Date of Patent: December 3, 2019
    Assignee: ModernaTX, Inc.
    Inventors: Antonin de Fougerolles, Justin Guild
  • Patent number: 10463751
    Abstract: The invention relates to compositions including polynucleotides encoding polypeptides which have been chemically modified by replacing the uridines with 1-methyl-pseudouridine to improve one or more of the stability and/or clearance in tissues, receptor uptake and/or kinetics, cellular access by the compositions, engagement with translational machinery, mRNA half-life, translation efficiency, immune evasion, protein production capacity, secretion efficiency, accessibility to circulation, protein half-life and/or modulation of a cell's status, function, and/or activity.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: November 5, 2019
    Assignee: ModernaTX, Inc.
    Inventors: Antonin De Fougerolles, Justin Guild
  • Patent number: 10420819
    Abstract: The invention provides non-hormonal vitamin D conjugated to insulin peptides that result in the peptides having increased absorption, bioavailability or circulating half-life when compared to non-conjugated forms. The vitamin D targeting groups are coupled to the insulin peptides via the third carbon on the vitamin D backbone.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: September 24, 2019
    Assignee: Extend Biosciences, Inc.
    Inventors: Tarik Soliman, Laura M. Hales, Daniel B. Hall, Howard Sard, Vishnumurthy Hegde
  • Patent number: 10253063
    Abstract: A method of purifying a desired protein from a preparation includes providing the preparation in a form having less than about 5% of the chromatin residing in the original production medium, contacting the preparation with a nonionic organic polymer in an amount sufficient to cause the desired protein to precipitate or adsorb on a nonionic hydrophilic surface, and adjusting a salt concentration before or during the contacting step, the adjusting step providing a sufficient salt concentration to produce a conductivity greater than physiological conductivity.
    Type: Grant
    Filed: February 27, 2014
    Date of Patent: April 9, 2019
    Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventor: Peter Stanley Gagnon
  • Patent number: 9624260
    Abstract: The present invention provides a process for the isolation of one or more proteins) from a protein solution. The process comprising the steps of: a) providing a protein solution comprising one or more specific proteins) and having a preset pH and a preset ionic strength or conductivity, b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and c) obtaining one or more proteins) from the column; wherein the protein solution has been supplemented with an alcohol.
    Type: Grant
    Filed: June 7, 2005
    Date of Patent: April 18, 2017
    Assignee: Therapure Biopharma Inc.
    Inventor: Allan Otto Fog Lihme
  • Patent number: 9616109
    Abstract: The invention provides non-hormonal vitamin D conjugated to insulin peptides that result in the peptides having increased absorption, bioavailability or circulating half-life when compared to non-conjugated forms. The vitamin D targeting groups are coupled to the insulin peptides via the third carbon on the vitamin D backbone.
    Type: Grant
    Filed: October 21, 2015
    Date of Patent: April 11, 2017
    Assignee: Extend Biosciences, Inc.
    Inventors: Tarik Soliman, Laura M. Hales, Daniel B. Hall, Howard Sard, Vishnumurthy Hegde
  • Patent number: 9289699
    Abstract: The current invention is directed to a chromatography column separator which separates the chromatography column in an upper chromatography column chamber and a lower chromatography column chamber, and which has a variable position within the chromatography column, and which is embedded by the chromatography material. The separator allows the replacement of the chromatography material in the upper chromatography column chamber without the need to replace the chromatography column material in the lower chromatography column chamber and it allows also the combination of two different chromatography materials with different chromatographical functional groups in one chromatography column.
    Type: Grant
    Filed: July 28, 2010
    Date of Patent: March 22, 2016
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Andreas Blaschyk, Roberto Falkenstein
  • Patent number: 9284375
    Abstract: The present invention is directed to diabody molecules and uses thereof in the treatment of a variety of diseases and disorders, including immunological disorders, infectious disease, intoxication and cancers. The diabody molecules of the invention comprise two polypeptide chains that associate to form at least two epitope binding sites, which may recognize the same or different epitopes on the same or differing antigens. Additionally, the antigens may be from the same or different molecules. The individual polypeptide chains of the diabody molecule may be covalently bound through non-peptide bond covalent bonds, such as, but not limited to, disulfide bonding of cysteine residues located within each polypeptide chain. In particular embodiments, the diabody molecules of the present invention further comprise an Fc region, which allows antibody-like functionality to engineered into the molecule.
    Type: Grant
    Filed: December 17, 2009
    Date of Patent: March 15, 2016
    Assignee: MacroGenics, Inc.
    Inventors: Leslie S. Johnson, Ling Huang
  • Patent number: 9255138
    Abstract: A method for extracting recombinant human serum albumin (rHSA) from transgenic rice grain is provided, comprising the steps of: 1) grinding dehusked rice containing rHSA into milled rice grain with a fineness of 80˜120 mesh, which is mixed with a extraction buffer in a w/v ratio of 1:5, then extracting at 55˜60° C. for 1˜3 hours to obtain mixture I; said extraction buffer comprises 10˜30 mM phosphate buffer, 10˜20 mM sodium acetate, 15˜30 mM ammonium sulfate and 5˜20 mM sodium caprylate and has a pH of 6.5˜8; 2) adjusting the pH of mixture I to 4.0˜4.5, followed by precipitating at room temperature for 3˜12 hours to obtain mixture II; 3) filtering the mixture II and collecting the filtrate, to obtain a solution containing high concentration of rHSA. The concentration of rHSA in the resultant solution is 650˜660 ?g/mL, which increases by 1.15 times comparing to the extraction amount before improvement, and the amount of non-target protein is reduced by 2.46 times.
    Type: Grant
    Filed: August 10, 2011
    Date of Patent: February 9, 2016
    Assignee: Healthgen Biotechnology Co., Ltd.
    Inventors: Daichang Yang, Yang He, Guangfei Li
  • Patent number: 9170260
    Abstract: Diagnostic tests for characterizing an individual's risk of developing or having a cardiovascular disease. In one embodiment the present diagnostic test comprises determining the level of myeloperoxidase (MPO) activity in a bodily sample obtained from the individual or test subject. In another embodiment, the diagnostic test comprises determining the level of MPO mass in a bodily sample obtained from the test subject. In another embodiment, the diagnostic test comprises determining the level of one or more select MPO-generated oxidation products in a bodily sample obtained from the test subject. The select MPO-generated oxidation products are dityrosine, nitrotyrosine, methionine sulphoxide or an MPO-generated lipid peroxidation products.
    Type: Grant
    Filed: May 25, 2007
    Date of Patent: October 27, 2015
    Assignee: The Cleveland Clinic Foundation
    Inventors: Stanley Hazen, Renliang Zhang
  • Patent number: 9163228
    Abstract: The present invention relates to a method for isolating and purifying nucleic acids, preferably comprising genomic DNA, from biological samples comprising the steps of lysing the sample using a lysis buffer comprising a source of anionic surfactant ions, optionally disintegrating the RNA present in the lysate, precipitating the surfactant ions from the lysate, and separating the nucleic acids from the precipitate and further contaminants by size-exclusion chromatography. The invention furthermore relates to a lysis buffer, a method of lysing cells and a kit for the isolation and purification of nucleic acids.
    Type: Grant
    Filed: April 8, 2011
    Date of Patent: October 20, 2015
    Assignee: QIAGEN GmbH
    Inventors: Roland Fabis, Markus Müller, Jörg Hucklenbroich, Mario Scherer
  • Publication number: 20140302554
    Abstract: A compound may generally comprise the formula: wherein R1 is independently selected from C2-C10 alkyl or substituted alkyl, R2 is independently selected from the group consisting of —H and C1-C6 alkyl or substituted alkyl, X is selected from the group consisting of —NH— and —O—, Y is a carbohydrate, and m is an integer from 1 to 8. The compound may comprise a non-ionic acid labile surfactant. The compound may be used to facilitate solubilization of proteins and other molecules in an aqueous environment.
    Type: Application
    Filed: June 23, 2014
    Publication date: October 9, 2014
    Inventors: Matthew Jacob Powell, Trust T. Razunguzwa, Miaosheng Li
  • Publication number: 20140296464
    Abstract: The present invention provides a chromatography medium comprising one or more electrospun polymer nanofibres which form a stationary phase comprising a plurality of pores through which a mobile phase can permeate and use of the same in chromatography, such as the isolation of recombinant proteins, monoclonal antibodies, viral vaccines and plasmid DNA. The invention further provides for the use of the chromatographic medium in a simulated moving bed system.
    Type: Application
    Filed: November 7, 2012
    Publication date: October 2, 2014
    Applicant: UCL BUSINESS PLC
    Inventors: Daniel Gilbert Bracewell, Robert Stevens, Oliver Hardick
  • Publication number: 20140194543
    Abstract: This invention concerns the preparation and use of novel polymeric chromatographic media and preferably mixed mode polymeric chromatographic media. In accordance with the present invention, polymeric media is prepared using polymeric particles derivatized with polyethyleneimine, and preferably such polyethyleneimine derivatized polymeric particles further functionalized with appropriate reactants. The polymeric chromatographic media is especially useful for bioseparations.
    Type: Application
    Filed: October 29, 2013
    Publication date: July 10, 2014
    Inventors: Nandu Deorkar, Robert C. Buss, Joseph M. Mladosich, Paul A. Bouis
  • Patent number: 8741662
    Abstract: Methods and kits for diagnosis and prognosis using biomarkers comprising albumin-bound protein/peptide complex (ABPPC).
    Type: Grant
    Filed: June 14, 2007
    Date of Patent: June 3, 2014
    Assignee: The Johns Hopkin University
    Inventors: Jennifer E. Van Eyk, Rebekah Lynn Gundry, Robert J. Cotter
  • Publication number: 20140128326
    Abstract: The present invention relates to variants of a parent albumin, the variants having altered plasma half-life compared with the parent albumin. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: November 8, 2013
    Publication date: May 8, 2014
    Applicants: Novozymes Biopharma DK A/S, University of OSLO, Novozymes Biopharma UK Limited, Novozymes A/S
    Inventors: Jason Cameron, Karen Ann Delahay, Jens Erik Nielsen, Andrew Plumridge, Jan Terje Andersen
  • Publication number: 20140121357
    Abstract: A method for producing injectable pharmaceutical formulations of blood-derived protein materials includes the steps of fractioning the source material in a polymer/salt aqueous two-phase system in the presence of phenol, purifying the top phase of the system by means of precipitation with caprylic acid and purifying the bottom phase by means of thermocoagulation, increasing the purity of the materials in both phases through chromatography, removing viral particles by means of the nanofiltration of both preparations, and formulating, stabilizing, and packaging the resulting materials.
    Type: Application
    Filed: April 8, 2011
    Publication date: May 1, 2014
    Applicant: UNIVERSIDAD DE COSTA RICA
    Inventors: Alvaro Segura Ruiz, Mariángela Vargas Arroyo, Guillermo Leon Montero, Mauren Villalta Arrieta, Maria Herrera Vega, Yamileth Angulo Ugalde
  • Publication number: 20140106371
    Abstract: Provided are peptide analogues of PA-IL and compositions containing them. The PA-IL peptide analogues have altered carbohydrate binding specificity relative to a PA-IL of SEQ ID NO:1, and thus the analogues contain amino acid substitutions in SEQ ID NO:1. The substitutions can be at positions 50, 52 and 53 of SEQ ID NO:1 and can include combinations of amino acid substitutions at those positions Also included are methods for detecting changes in the glycosylation of carbohydrates and for separating biomolecules which contain glycoproteins or glycoconjugates.
    Type: Application
    Filed: July 2, 2013
    Publication date: April 17, 2014
    Applicant: Dublin City University
    Inventors: Paul Clarke, Roisin Thompson, Brendan O'Connor, Michael O'Connell, Kenneth McMahon
  • Publication number: 20140087445
    Abstract: Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed.
    Type: Application
    Filed: November 27, 2013
    Publication date: March 27, 2014
    Applicant: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Catherine A. Bothof, Yi He, Jerald K. Rasmussen, Kannan Seshadri, Clinton P. Waller, JR., Douglas E. Weiss
  • Publication number: 20130309169
    Abstract: The present invention relates to a sentinel lymph node marker comprising an albumin; a radioactive isotope and/or near infrared dye which is bound to the albumin; and a visible dye which is bound to the albumin, a preparation method thereof, a kit for multimode imaging of a sentinel lymph node to prepare the sentinel lymph node marker, and a method for multimode imaging of a sentinel lymph node using the same. The sentinel lymph node marker of the invention remains in the sentinel lymph node for a long period of time and allows for multimode imaging of the sentinel lymph node. Thus, using this marker the sentinel lymph node can be accurately identified in vivo by near infrared imaging and/or gamma imaging without incision of skin, and the location of the identified sentinel lymph node can be precisely identified with the naked eye during a surgical operation of removing the identified sentinel lymph node.
    Type: Application
    Filed: August 16, 2011
    Publication date: November 21, 2013
    Applicant: NATIONAL CANCER CENTER
    Inventors: Seok Ki Kim, Se Hun Kang, Seo Il Kim, Young Sang Kim, Nam Suk Baek, Jin Hee Noh
  • Publication number: 20130178603
    Abstract: The various embodiments herein provide a method of synthesizing a multi-mode cancer targeted nanoparticles. The method comprises the steps of preparing a plurality of nanoparticles and covalently conjugating monoclonal antibodies on surface of the prepared plurality of nanoparticles. The plurality of nanoparticles consists of a protein and a drug. The protein is Human Serum Albumin protein (HSA) and the drug is methotrexate. The monoclonal antibodies are anti-MUC1 nanobodies. According to an embodiment herein, a multi-mode cancer targeted nanoparticles comprising a plurality of cross linked nanoparticles of protein and drug molecules and covalently linked molecules of monoclonal antibodies. The molecules of monoclonal antibodies are linked on a surface of the plurality of cross linked nanoparticles.
    Type: Application
    Filed: January 11, 2012
    Publication date: July 11, 2013
    Inventors: Rassoul Dinarvand, Mohammad Ali Derakhshan, Fatemeh Rahbarizadeh, Reza Faridi Majidi, Azade Taheri Borujeni, Seyed Mahdi Rezayat
  • Publication number: 20130090397
    Abstract: Semi-interpenetrating polymeric networks are described. More specifically, the semi-interpenetrating polymeric networks include at least two polymers that are closely associated. The first polymer is an ionic polymer that is not crosslinked. The second polymer is a cross-linked polymer that can be either another ionic polymer or a non-ionic polymer. Methods of making the semi-interpenetrating polymeric networks, articles containing the semi-interpenetrating polymeric networks, and methods of using the semi-interpenetrating polymeric networks are also described. The semi-interpenetrating polymeric networks can function as ion exchange resins.
    Type: Application
    Filed: June 17, 2011
    Publication date: April 11, 2013
    Applicant: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Del R. Lawson, James I. Hembre, Jerald K. Rasmussen, Catherine A. Bothof, Kannan Seshadri, Robert T. Fitzsimons, JR., Andrew W. Rabins, Taib K. Ansera
  • Publication number: 20130023444
    Abstract: Provided herein are novel reagents and their use in color-producing detection systems for performing diagnostic tests and analytical assays.
    Type: Application
    Filed: May 23, 2012
    Publication date: January 24, 2013
    Applicant: Diagnostic Innovations, LLC
    Inventor: Victor Kovalenko
  • Patent number: 8303922
    Abstract: A new method is disclosed for the exfoliation of hexagonal boron nitride into mono- and few-layered nanosheets (or nanoplatelets, nanomesh, nanoribbons). The method does not necessarily require high temperature or vacuum, but uses commercially available h-BN powders (or those derived from these materials, bulk crystals) and only requires wet chemical processing. The method is facile, cost efficient, and scalable. The resultant exfoliated h-BN is dispersible in an organic solvent or water thus amenable for solution processing for unique microelectronic or composite applications.
    Type: Grant
    Filed: August 24, 2009
    Date of Patent: November 6, 2012
    Assignee: The United States of America as represeted by the Administrator of the National Aeronautics and Space Administration
    Inventors: Yi Lin, John W. Connell
  • Publication number: 20120264158
    Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilization of proteins and other molecules in an aqueous environment.
    Type: Application
    Filed: June 25, 2012
    Publication date: October 18, 2012
    Applicant: Protea Biosciences, Inc.
    Inventors: Matthew Jacob Powell, Trust Tariro Razunguzwa, George Augustine O'Doherty, Miaosheng Li
  • Publication number: 20120264157
    Abstract: Anionic acid-labile surfactants may generally comprise compounds represented by the formula: wherein R1 is independently selected from —(CH2)0-9CH3, R2 is selected from the group consisting of —H and —(CH2)0-5CH3, Y is an anion, X is a cation, and n is an integer from 1 to 8. Methods of making and using the anionic acid-labile surfactants are also described. The anionic acid-labile surfactants may be used to facilitate the solubilization of proteins and other molecules in an aqueous environment.
    Type: Application
    Filed: June 25, 2012
    Publication date: October 18, 2012
    Applicant: Protea Biosciences, Inc.
    Inventors: Matthew Jacob Powell, Trust Tariro Razunguzwa, George Augustine O'Doherty, Miaosheng Li
  • Publication number: 20120258519
    Abstract: Methods of harvesting proteins directly from bioreactors to avoid at several steps in the purification of recombinant drugs are disclosed.
    Type: Application
    Filed: April 10, 2011
    Publication date: October 11, 2012
    Applicant: Therapeutic Proteins Inc.
    Inventor: Sarfaraz K. Niazi
  • Publication number: 20120165509
    Abstract: A method for separating and purifying recombinant human serum albumin (rHSA) from transgenic rice grain, sequentially comprising the steps of: 1) subjecting crude extract of rHSA to cation exchange chromatography to obtain primary product I; 2) subjecting the primary product I to anion exchange chromatography to obtain secondary product II; 3) subjecting the secondary product II to hydrophobic chromatography to obtain purified rHSA. The method may further comprise a step of ceramic hydroxyapatite chromatography prior to the hydrophobic chromatography. The method has the advantages of low cost and easy operation. The resultant rHSA has a purity of about 99% by HPLC.
    Type: Application
    Filed: August 10, 2011
    Publication date: June 28, 2012
    Applicant: Healthgen Biotechnology Co., Ltd.
    Inventors: Daichang Yang, Yang He, Guangfei Li, Jingru Liu
  • Publication number: 20120149873
    Abstract: An improved method for purifying albumin, a variant or fragment thereof, a fusion protein comprising albumin, a variant or fragment thereof, or a conjugate comprising albumin, a variant or fragment thereof is disclosed.
    Type: Application
    Filed: May 7, 2010
    Publication date: June 14, 2012
    Applicant: NOVOZYMES BIOPHARMA DK A/S
    Inventors: Lee Edward Blackwell, Jason Cameron, Phillip Harvey Morton
  • Publication number: 20120077249
    Abstract: A method of purifying biomolecules with an anion exchanger containing a membrane having a surface having a polymer such as a primary or secondary amine ligand formed thereon, such as polyallylamine. The feedstock is introduced to the exchanger in the presence of one or more ionic-modifiers by themselves or in combination with monovalent salt. The ionic modifier alters the binding ability of the primary amines such that they retain a significant binding capacity for highly charged species such as DNA but lose part or almost all of their binding capacity for less charged species such as viruses or proteins at pH above the pI of the virus or protein.
    Type: Application
    Filed: March 29, 2011
    Publication date: March 29, 2012
    Applicant: MILLIPORE CORPORATION
    Inventors: Senthil Ramaswamy, Ganesh Iyer, Steve Cross
  • Publication number: 20120046231
    Abstract: Described herein is a composition and process for synthesizing a human serum albumin (HSA) based plasma replacement composition that includes a polymerized HSA (PolyHSA) that is chemically stabilized by the reduction of Schiff bases. The PolyHSA may have a molecular weight of at least about 100 kDa and may optionally have a cross-linker to HSA molar ratio of at least about 10:1. The PolyHSA composition is useful for restoring a subject's circulatory volume.
    Type: Application
    Filed: August 23, 2011
    Publication date: February 23, 2012
    Inventors: Andre Francis Palmer, Pedro Cabrales
  • Patent number: 8114963
    Abstract: Fluorescent dye compounds of formula (I) are disclosed. These compounds are useful as they interact with organic compounds in a manner such that excitation with certain wavelengths of light results in fluorescent emission. Detection and/or monitoring of the fluorescence provides a means for the detection or quantification of organic compounds when bound to these fluorescent dye compounds. Formula (I), wherein: each of R, R? and R? is a hydrogen atom, halogen atom or a straight or branched C1-20 alkyl, alkenyl or alkynl group optionally substituted with one or more halogen, hydroxyl, and/or oxy group; rings A, B and C optionally include one or more double bonds; rings B and C are optionally substituted with one or more halogen atoms.
    Type: Grant
    Filed: December 3, 2007
    Date of Patent: February 14, 2012
    Assignee: Flurotechnics Pty Limited
    Inventors: Philip John Livingston Bell, Peter Karuso
  • Publication number: 20120022234
    Abstract: The present invention relates to a method for separating albumin conjugate from unconjugated albumin in a solution comprising albumin conjugate and unconjugated albumin by loading the solution onto a hydrophobic support equilibrated in aqueous buffer having a high salt content; applying to the support a gradient of decreasing salt concentration; and collecting the eluted albumin conjugate.
    Type: Application
    Filed: September 17, 2010
    Publication date: January 26, 2012
    Inventors: Nathalie Bousquet-Gagnon, Omar Quraishi, Dominique P. Bridon
  • Patent number: 8084580
    Abstract: The invention discloses a purified albumin solution of human origin with low prekallicrein activator (PKA) activity and stability over time characterized in that it has an antithrombin content equal to or greater than 0.03 mg/g of albumin, and a process for production thereof by the partial extraction of the antithrombin during fractionation of the human plasma.
    Type: Grant
    Filed: March 21, 2007
    Date of Patent: December 27, 2011
    Assignee: Grifols, S.A.
    Inventors: Juan Ignacio Jorquera Nieto, Nuria Hosta Mateu, Olga Santaeularia Lozano
  • Publication number: 20110311505
    Abstract: This invention is related to preparation of photosensitive ruthenium based aminoacid monomers and oligomers, aminoacid monomer-protein cross-linking using photo sensitat ion and conjugation on micro and nano-structures by ruthenium-chelate based monomers. Its vast range biotechnolgy applications of multifunctional, biocompatible, stabilE and specific micro and nanobio-conjugates, which will stand-alone or simultaneously enable (i) both purification and determination, (ii) both targeting and imaging and theranostics and (iii) catalysis and determination. The construction and method of preparation is applicable to silica materials, superparamagnetic particles, QDs, CNTs, Ag/Au nanoparticles and Au surfaces and polymeric materials. The photosensitive aminoacid monomer linkers can react via chemically and biocompatible to a lot of different micro and nano-surface and then to the protein when they act as a single-step cross-linking reaction using irradiation.
    Type: Application
    Filed: December 11, 2009
    Publication date: December 22, 2011
    Inventors: Arzu Ersoz, Deniz Hur, Filiz Yilmaz, Adil Denizli, Ayca Atilir Ozcan, Sibel Emir Diltemiz, Suzan Yazar, Ozlem Bicen, Sibel Buyuktiryaki, Rustem Kecili, Guner Saka, Tugba Findik
  • Publication number: 20110313133
    Abstract: Based on the three-dimensional structure of albumin, the inventors have designed variant polypeptides (muteins) which have one or more cysteine residues with a free thiol group (hereinafter referred to as “thio-albumin”). The variant polypeptide may be conjugated through the sulphur atom of the cysteine residue to a conjugation partner such as a bioactive compound.
    Type: Application
    Filed: February 11, 2010
    Publication date: December 22, 2011
    Applicants: Novozymes A/S, Novozymes BioPharma DK A/S
    Inventors: Christopher John Arthur Finnis, Joanna Mary Hay, Esben Peter Friis, Jason Cameron, Darrell Sleep
  • Publication number: 20110312027
    Abstract: Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.
    Type: Application
    Filed: December 9, 2009
    Publication date: December 22, 2011
    Inventors: Travis Young, Peter G. Schultz
  • Publication number: 20110281283
    Abstract: The present invention relates to animal feed additives and detection thereof in feed products. Additionally, the present invention relates to yeast cell wall components, their methods of isolation, and compositions and methods for the immunological detection thereof.
    Type: Application
    Filed: May 13, 2011
    Publication date: November 17, 2011
    Applicant: ALLTECH, INC.
    Inventors: Colm Moran, Stefan Kwiatkowski, Alexandros Yiannikouris, Ursula Anne Thielen
  • Patent number: 7993877
    Abstract: A process is provided for the preparation of a highly pure recombinant albumin solution having a nickel ion content of less than 100 ng per gram of albumin. The process comprises subjecting a recombinant albumin to a series of chromatography, concentration, and diafiltration steps.
    Type: Grant
    Filed: September 9, 2008
    Date of Patent: August 9, 2011
    Assignee: Novozymes Biopharma DK A/S
    Inventors: Hendrik Van Urk, David John Mead, Philip Harvey Morton, Andrew John Cartwright, Jason Cameron, David James Ballance, Michel Gaston Joseph Grandgeorge, Stephen Berezenko, John Rodney Woodrow, Darrell Sleep, Jean-Luc Bernard Veron
  • Publication number: 20110190813
    Abstract: A method of crosslinking a protein or peptide for use as a biomaterial, the method comprising the step of irradiating a photoactivatable metal-ligand complex and an electron acceptor in the presence of the protein or peptide, thereby initiating a crosslinking reaction to form a 3-dimensional matrix of the biomaterial.
    Type: Application
    Filed: August 14, 2008
    Publication date: August 4, 2011
    Inventors: Alan George Brownlee, Christopher Malcolm Elvin, Jerome Anthony Werkmeister, John Alan Maurice Ramshaw, Charles Mark Lindall
  • Publication number: 20110087009
    Abstract: A process for the production of a microparticle or a nanoparticle of a chemical compound comprising the steps of providing a solution of said chemical compound in a first liquid; providing a second liquid in which said chemical compound is insoluble or substantially insoluble; combining said liquids in a region of high shear thereby causing formation of said particles; and isolating said particles of said compound. The processing time of a coacervation style process can be reduced and the yield can be substantially increased both by control of the precipitation step which allows for desolvation step to be dispensed with leading to significant process time reduction. The invention also provides a molecular mixing unit comprising an outer body defining a mixing zone; a shear means to provide shear liquid in said mixing zone; at least one fluid inlet means for a first liquid; at least one fluid inlet means for a second liquid and a fluid outlet means.
    Type: Application
    Filed: December 16, 2010
    Publication date: April 14, 2011
    Inventors: Sung Lai Jimmy YUN, Jian Feng Chen
  • Publication number: 20110045223
    Abstract: A new method is disclosed for the exfoliation of hexagonal boron nitride into mono- and few-layered nanosheets (or nanoplatelets, nanomesh, nanoribbons). The method does not necessarily require high temperature or vacuum, but uses commercially available h-BN powders (or those derived from these materials, bulk crystals) and only requires wet chemical processing. The method is facile, cost efficient, and scalable. The resultant exfoliated h-BN is dispersible in an organic solvent or water thus amenable for solution processing for unique microelectronic or composite applications.
    Type: Application
    Filed: August 24, 2009
    Publication date: February 24, 2011
    Applicant: United States of America as represented by the Administrator of the National Aeronautics and Spac
    Inventors: Yi Lin, John W. Connell
  • Patent number: 7893212
    Abstract: The present invention provides an S-nitroso group-containing albumin, comprising an S-nitroso group introduced to at least one lysine in the amino acid sequence for albumin. The S-nitro group has been introduced into a lysine in the S-nitroso group-containing albumin of the invention to allow more S-nitroso groups to be included in the albumin, thus ensuring more potent inhibition of cancer cells in NO groups.
    Type: Grant
    Filed: August 29, 2008
    Date of Patent: February 22, 2011
    Assignee: Nipro Corporation
    Inventors: Masaki Otagiri, Keisuke Nakajo, Naohisa Katayama, Toshiya Kai
  • Publication number: 20100233783
    Abstract: Process for purifying a recombinant protein including one or a few procedural steps only. The process combines the step of lysis of the host cell, with the purification of the protein of interest, allowing for a rapid and much more efficient process of purification. The conditions used during the purification process are those of a high temperature and a low pH, allowing for thermostable and acid-resistant recombinant proteins to be isolated from a suspension. The invention also relates to purifying recombinant proteins which are fusion proteins, wherein one part of the protein may be selected from an enamel matrix protein, such as amelogenin.
    Type: Application
    Filed: March 23, 2006
    Publication date: September 16, 2010
    Applicant: Straumann Holding AG
    Inventors: Johan Per Henrik Svensson, Leif Bülow
  • Publication number: 20100167374
    Abstract: The present invention relates to a chromatographic method of separating biological material comprising, providing chromatographic media comprising inorganic oxide particles having an average diameter of about 2 microns or less and an average pore diameter of 300 ? or more; applying a solvent comprising said biological material to said media, wherein said biological material is reversibly bonded to said media; and eluting said biological material from said media with a solvent in less than about 2 minutes for biological material having a molecular weight of less than about 100,000 Daltons.
    Type: Application
    Filed: February 21, 2008
    Publication date: July 1, 2010
    Inventors: Reno T. Nguyen, Scott Anderson, Ian Chappell
  • Patent number: 7696176
    Abstract: A composition comprising Human Serum Albumin and an Amino Acid Solution, a method of making the same, and a method for use, including treating acute hypovolemia due to any number of medical conditions due to sepsis with shock, hemorrhagic shock, hypovolemic shock, burn injury, capillary leak syndrome, hypoalbuminemia, nephritic syndrome, or multi-organ failure with third space fluid loss from any other medical disease.
    Type: Grant
    Filed: May 31, 2007
    Date of Patent: April 13, 2010
    Inventor: William J. Norberg
  • Publication number: 20100004178
    Abstract: A composition comprising an albumin-based colloid and its use in treating hypovolemic conditions such as capillary leak syndrome and shock are disclosed.
    Type: Application
    Filed: September 10, 2009
    Publication date: January 7, 2010
    Inventors: Ragheb Assaly, John D. Dignam, Joseph I. Shapiro
  • Publication number: 20090306346
    Abstract: The invention relates to a method for separating proteins from liquid media, comprising providing a liquid medium containing proteins, providing a clay material which has: a specific surface greater than 150 m2/g, a pore volume greater than 0.35 ml/g, an ion exchange capacity greater than 40 meq/100 g, and a sediment volume in water of less than 15 ml/2 g, equilibrating said clay material to a pH of from 3.5 to 9.0, treating said liquid medium with the equilibrated clay material, and separating the purified, protein-depleted liquid medium from the clay material.
    Type: Application
    Filed: December 15, 2006
    Publication date: December 10, 2009
    Applicant: SUD-CHEMIE AG
    Inventors: Ulrich Sohling, Thomas Scheper, Kaspar Cornelia, Daniel Riechers, Arne Burzlaff