Derived From Fungi, E.g., Yeasts, Mushroom, Etc. Patents (Class 530/371)
  • Patent number: 9493505
    Abstract: The present invention provides novel conformationally-defined macrocyclic compounds that have been demonstrated to be selective modulators of the ghrelin receptor (growth hormone secretagogue receptor, GHS-R1a and subtypes, isoforms and variants thereof). Methods of synthesizing the novel compounds are also described herein. These compounds are useful as agonists of the ghrelin receptor and as medicaments for treatment and prevention of a range of medical conditions including, but not limited to, metabolic and/or endocrine disorders, gastrointestinal disorders, cardiovascular disorders, obesity and obesity-associated disorders, central nervous system disorders, genetic disorders, hyperproliferative disorders and inflammatory disorders.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: November 15, 2016
    Assignee: OCERA THERAPEUTICS, INC.
    Inventors: Eric Marsault, Carl St-Louis
  • Patent number: 9207240
    Abstract: Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.
    Type: Grant
    Filed: November 14, 2007
    Date of Patent: December 8, 2015
    Assignee: ARBOR VITA CORPORATION
    Inventors: Adrien P. Malick, Virginia M. Crews, Julie L. Rosales, Carrie S. Ferguson, Jeff H. Bruton, Robert J. Beadenkopf, John Mantlo
  • Patent number: 9181298
    Abstract: The present invention is directed to novel macrocyclic compounds of formula (I) and their pharmaceutically acceptable salts, hydrates or solvates: wherein R1, R2, R3, R4, R5, R6, n1, m, p Z1, Z2, and Z3 are as describe in the specification. The invention also relates to compounds of formula (I) which are antagonists of the motilin receptor and are useful in the treatment of disorders associated with this receptor and with or with motility dysfunction.
    Type: Grant
    Filed: November 19, 2008
    Date of Patent: November 10, 2015
    Assignee: OCERA THERAPEUTICS, INC.
    Inventors: Eric Marsault, Kamel Benakli, Hamid Hoveyda, Mark Peterson, Sylvie Beaubien, Luc Oullet, Carl St-Louis, Sophie Beauchemin
  • Patent number: 8999696
    Abstract: The present invention relates to methods for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain. The present invention also relates to such cellulolytic enzyme compositions.
    Type: Grant
    Filed: May 9, 2008
    Date of Patent: April 7, 2015
    Assignee: Novozymes, Inc.
    Inventor: Keith McFarland
  • Publication number: 20150074842
    Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the family Phacosporaceae plants and/or plant cells. This is achieved for instance by increasing the expression of a hydrophobin protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. In the transgenic plants hydrophobin can be expressed as a fusion protein to facilitate and/or enhance expression. Furthermore, the hydrophobin protein can be expressed including a secretion signal sequence which mediates secretion of the protein into the apoplast and/or into the cuticule.
    Type: Application
    Filed: March 15, 2013
    Publication date: March 12, 2015
    Inventors: Holger Schultheiss, Ralf Flachmann, Thomas Subkowski, Claus Bollschweiler
  • Patent number: 8957190
    Abstract: The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70° C. or more.
    Type: Grant
    Filed: July 11, 2013
    Date of Patent: February 17, 2015
    Assignee: Japan Tobacco Inc.
    Inventors: Yoshimitsu Takakura, Satoru Usami, Masako Ichikawa
  • Patent number: 8921521
    Abstract: The present invention provides novel conformationally-defined macrocyclic compounds that have been demonstrated to be selective modulators of the ghrelin receptor (growth hormone secretagogue receptor, GHS-R1a and subtypes, isoforms and variants thereof). Methods of synthesizing the novel compounds are also described herein. These compounds are useful as agonists of the ghrelin receptor and as medicaments for treatment and prevention of a range of medical conditions including, but not limited to, metabolic and/or endocrine disorders, gastrointestinal disorders, cardiovascular disorders, obesity and obesity-associated disorders, central nervous system disorders, genetic disorders, hyperproliferative disorders and inflammatory disorders.
    Type: Grant
    Filed: December 17, 2012
    Date of Patent: December 30, 2014
    Assignee: Ocera Therapeutics, Inc.
    Inventors: Eric Marsault, Carl St-Louis
  • Patent number: 8877915
    Abstract: Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: November 4, 2014
    Assignees: National Institute of Advanced Industrial Science and Technology, Noda Institute for Scientific Research
    Inventors: Masahiro Ogawa, Yasuji Koyama, Masayuki Machida
  • Publication number: 20140248689
    Abstract: The present invention relates to a glucose-induced inactivation/degradation-resistant transporter gene and use thereof, and more particularly, to a brewing yeast having excellent assimilation of oligosaccharides (maltose, maltotriose, etc.), an alcoholic beverage prepared using the yeast, a method of producing the alcoholic beverage, etc. More specifically, the present invention relates to a glucose-induced inactivation/degradation-resistant transporter including Mal21p, mutant Mal31p, mutant Mal61p, mutant Mtt1p, mutant Agt1p, etc., a gene encoding the transporter, a method of producing an alcoholic beverage using thereof, and so on.
    Type: Application
    Filed: April 4, 2014
    Publication date: September 4, 2014
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventors: Haruyo HATANAKA, Fumihiko OMURA
  • Publication number: 20140221270
    Abstract: Methoxypolyethyleneglycol succinimidyl propionate modified recombinant ganoderma immunoregulatory protein, a preparing method and applications thereof are provided, including: the mPEG-SPA modified rLZ-8; the method for preparing the mPEG-SPA modified rLZ-8 comprising: feeding the rLZ-8 dimer and the mPEG-SPA with the molar ratio of 1:1˜1:6 into a 0.1M phosphate buffer with pH 5.0˜pH 8.0, and stirring by a magnetic stirrer at a room temperature for 1.0˜2.5 h, purifying the product for obtaining a modification product with a purity of 98%; and applications of the mPEG-SPA modified rLZ-8 in preparation of medicine for treating leukopenia due to chemotherapy. Advantages are as follows: the method for preparing the mPEG-SPA modified rLZ-8 is simple, and the product is identical; a half-life of the mPEG-SPA modified rLZ-8 is significantly longer than the half-life of unmodified rLZ-8 (illustrated in the FIG. 2); a minimum effective dosage and time for treating leucopenia are also improved.
    Type: Application
    Filed: June 3, 2013
    Publication date: August 7, 2014
    Inventor: Xitian Zhang
  • Publication number: 20140220069
    Abstract: An active ingredient derived from Torulaspora delbrueckii and the use thereof for improving and/or restoring the barrier function of the skin. Also, cosmetic compositions containing this active ingredient and a cosmetic care process for improving and/or restoring the barrier function of the skin.
    Type: Application
    Filed: September 4, 2012
    Publication date: August 7, 2014
    Applicant: SOCIETE INDUSTRIELLE LIMOUSINE D'APPLICATION BIOLOGIQUE
    Inventor: Jean Paufique
  • Patent number: 8633030
    Abstract: The present invention relates to isolated polypeptides having carboxypeptidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: December 8, 2009
    Date of Patent: January 21, 2014
    Assignee: Novozymes, Inc.
    Inventor: Michael Rey
  • Publication number: 20140005362
    Abstract: The subject of the present invention is a method of modifying proteinaceous toxins through the addition of an NLS motif. The resulting cytotoxin facilitates the selective elimination of proliferating cells, particularly tumour cells.
    Type: Application
    Filed: December 29, 2011
    Publication date: January 2, 2014
    Inventors: Anna Bujko, Magdalena Lukasiak, Jaroslaw Dastych, Miroslawa Skupinska, Ewelina Rodakowska, Leszek Rychlewski
  • Publication number: 20130309765
    Abstract: The present invention provides modified tamabidin 2, which is a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one or more amino acid mutations in the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence having an identity of not less than 80% to the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity, wherein an asparagine residue at position 115 of SEQ ID NO: 2 is replaced with cysteine. The modified biotin-binding protein has remarkable heat resistance.
    Type: Application
    Filed: December 28, 2011
    Publication date: November 21, 2013
    Applicant: JAPAN TOBACCO INC.
    Inventors: Yoshimitsu Takakura, Naomi Oka
  • Patent number: 8575320
    Abstract: The invention relates to soluble selenium compositions and methods of production, separation and purification thereof. In particular the present invention provides methods of preparing water soluble selenoglycoproteins (e.g., via extracting selenoglycoproteins from selenium enriched yeast), methods of supplementing a selenium deficient composition via admixing water soluble selenoglycoproteins with the selenium deficient composition, compositions comprising the water soluble selenoglycoproteins and methods of administering the same.
    Type: Grant
    Filed: September 16, 2011
    Date of Patent: November 5, 2013
    Assignee: Alltech, Inc.
    Inventors: Stefan Kwiatkowski, Ronan Power, Clayton Matney, Paiman Peter Ghoroghchian, Eric Michael Ostertag
  • Publication number: 20130217076
    Abstract: The saccharification efficiency of cellulase is enhanced within reaction temperature regions of general fermenting microorganisms other than heat-resistant yeast. Cellulosic biomass is saccharified with cellulase in the presence of a polypeptide comprising the amino acid sequence shown in SEQ ID NO: 2 or 4 and having a function of enhancing the saccharifying activity of cellulase.
    Type: Application
    Filed: February 15, 2013
    Publication date: August 22, 2013
    Inventors: Noriko SHISA, Nobuhiro ISHIDA, Chie IMAMURA, Shigeharu MORIYA, Jun KIKUCHI
  • Publication number: 20130219559
    Abstract: A novel method to produce hydrophobin monomers or multimers in plant tissues is disclosed. A novel method to produce hydrophobin multimers in E. coli cells is also described. The disclosure provides transgenic plants, transgenic seeds, a production system, and expression cassettes for making the transgenic plant carrying hydrophobin encoding gene sequences.
    Type: Application
    Filed: February 21, 2013
    Publication date: August 22, 2013
    Inventor: Kimmo Koivu
  • Patent number: 8226954
    Abstract: The present invention relates to a polypeptide possessing a CDase activity, characterized in that it is derived from a native CDase by addition of an amino acid sequence with the proviso that said polypeptide has no UPRtase or Thymidine Kinase activity.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: July 24, 2012
    Assignee: Transgene S.A.
    Inventor: Philippe Erbs
  • Publication number: 20120156173
    Abstract: The present disclosure relates to a novel ascomyceteous fungus, Sphaerodes mycoparasitica, for controlling plant fungal pathogens, disease symptoms, and mycotoxins in planta and ex planta. Uses, methods, compositions, sequences, and products are also disclosed herein.
    Type: Application
    Filed: February 27, 2012
    Publication date: June 21, 2012
    Inventor: Vladimir Vujanovic
  • Publication number: 20120142051
    Abstract: Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.
    Type: Application
    Filed: March 2, 2010
    Publication date: June 7, 2012
    Applicant: Noda Institute for Science Research
    Inventors: Masahiro Ogawa, Yasuji Koyama, Masayuki Machida
  • Publication number: 20120070561
    Abstract: An objective to be achieved by the present invention is to provide a method for producing a heat processed food which can be uniformly heated in a short time without causing “unevenness of heating” on microwave oven cooking. Another objective of the present invention is also to provide a heat professed food, use of an antifreeze protein in producing a heat processed food, a method for improving an effect of heating treatment on a heat professed food, and an agent for improving an effect of heating treatment. The method for producing a heat processed food according to the present invention is characterized in comprising the steps of adding an antifreeze protein to a food material or a food; and cooling the resulting mixture.
    Type: Application
    Filed: May 17, 2010
    Publication date: March 22, 2012
    Inventors: Naoki Arai, Katsuaki Abe, Jun Tomono, Shinichi Yokota, Hideaki Kegasa
  • Publication number: 20120003259
    Abstract: We describe an Fve polypeptide being a fragment, homologue, variant or derivative of Fve protein, which comprises at least one biological activity of Fve protein. uses of such a polypeptide, etc, and nucleic acids encoding these, in the treatment and prevention of allergy and cancer are also disclosed.
    Type: Application
    Filed: June 30, 2011
    Publication date: January 5, 2012
    Applicants: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH, NATIONAL UNIVERSITY OF SINGAPORE
    Inventors: Kaw Yan Chua, See Voon Seow, Prasanna Ratnakar Kolatkar
  • Publication number: 20110263824
    Abstract: The present invention relates to a modified biotin-binding protein. The modified biotin-binding protein of the present invention includes an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one to several amino acid mutations in the sequence represented by SEQ ID NO: 2, or an amino acid sequence having 80% or more identity to the sequence represented by SEQ ID NO: 2, and having a biotin-binding activity, wherein at least one residue selected from the group consisting of: 1) an arginine residue at position 104 of SEQ ID NO: 2; 2) a lysine residue at position 141 of SEQ ID NO: 2; 3) a lysine residue at position 26 of SEQ ID NO: 2; and 4) a lysine residue at position 73 of SEQ ID NO: 2 is replaced with an acidic amino acid residue or a neutral amino acid residue.
    Type: Application
    Filed: August 13, 2009
    Publication date: October 27, 2011
    Inventors: Yoshimitsu Takakura, Masako Ichikawa
  • Publication number: 20110252509
    Abstract: This invention relates generally to a method for producing a transgenic cell with increased gamma-aminobutyric acid (GABA) content as compared to a corresponding non-transformed wild type cell.
    Type: Application
    Filed: October 23, 2009
    Publication date: October 13, 2011
    Applicant: BASF Plant Sceince GmbH
    Inventors: Janneke Hendriks, Hardy Schön, Oliver Thimm, Volker Haake, Gunnar Plesch, Piotr Puzio, Astrid Blau, Michael Manfred Herold, Birgit Wendel, Beate Kamlage, Florian Schauwecker
  • Patent number: 8008440
    Abstract: A library of macrocyclic compounds of the formula (I) where part (A) is a ?bivalent radical, a —(CH2)y— bivalent radical or a covalent bond; where part (B) is a ?bivalent radical, a —(CH2)z— bivalent radical, or a covalent bond; where part (C) is a ?bivalent radical, a —(CH2)t— bivalent radical, or a covalent bond; and where part (T) is a —Y-L-Z- radical wherein Y is CH2 or CO, Z is NH or O and L is a bivalent radical. These compounds are useful for carrying out screening assays or as intermediates for the synthesis of other compounds of pharmaceutical interest. A process for the preparation of these compounds in a combinatorial manner, is also disclosed.
    Type: Grant
    Filed: December 22, 2006
    Date of Patent: August 30, 2011
    Assignee: Tranzyme Pharma Inc.
    Inventors: Pierre Deslongchamps, Yves Dory, Gilles Berthiaume, Luc Ouellet, Ruoxi Lan
  • Publication number: 20110173715
    Abstract: This invention relates generally to nucleic acid sequences encoding proteins that are associated with abiotic stress responses and abiotic stress tolerance in plants. In particular, this invention relates to nucleic acid sequences encoding proteins that confer drought, heat, cold, and/or salt tolerance to plants.
    Type: Application
    Filed: September 23, 2005
    Publication date: July 14, 2011
    Applicant: BASF PLANT SCIENCE GMBH
    Inventors: Piotr Puzio, Agnes Chardonnens, Harry Wild, Gunnar Plesch, Bryan McKersie
  • Publication number: 20110129938
    Abstract: Disclosed is a novel lectin which can bind specifically to an L-fucose ?1?6 sugar chain. Also disclosed is use of the lectin. The L-fucose ?1?6 specific lectin of the present invention is characterized in that: (1) the lectin is extracted from a basidiomycete or an ascomycete; (2) the lectin has a molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of 4,000 to 40,000; and (3) the lectin has an affinity to an L-fucose ?1?6 sugar chain, the affinity being represented by an association constant of 1.0×104M?1 or more (at 25 degrees C.). The lectin can be used for detecting an L-fucose ?1?6 sugar chain specifically, and is effective for the purification of an L-fucose ?1?6 sugar chain or a non L-fucose ?1?6 sugar chain.
    Type: Application
    Filed: July 15, 2009
    Publication date: June 2, 2011
    Inventors: Yuka Kobayashi, Jun Hirabayashi, Hiroaki Tateno, Hirokazu Kawagishi, Hideo Dohra
  • Publication number: 20110092675
    Abstract: This document relates to methods and materials involved in fungus-induced inflammation and eosinophil degranulation. For example, isolated nucleic acids encoding fungal polypeptides, fungal polypeptides, methods for assessing fungus-induced inflammation, methods for assessing eosinophil degranulation, and methods for identifying inhibitors of fungus-induced inflammation and/or eosinophil degranulation are provided.
    Type: Application
    Filed: December 20, 2010
    Publication date: April 21, 2011
    Inventors: Hirohito Kita, Jens Ponikau, Christopher Lawrence
  • Publication number: 20110086768
    Abstract: Fusion proteins comprising a transferrin moiety and integrin binding domain and peptide libraries thereof are disclosed. The present invention includes a method of screening transferrin and integrin peptide libraries displayed in fusion proteins expressed by host cells. The fusion proteins of the present invention include transferrin fusion proteins capable of expression in yeast.
    Type: Application
    Filed: December 7, 2007
    Publication date: April 14, 2011
    Applicant: BIOREXIS PHARMACEUTICAL CORPORATION
    Inventors: Andrew John Turner, Baiyang Wang
  • Publication number: 20110082074
    Abstract: The present invention provides a pharmaceutical agent that is useful for preventing periodontal disease. The pharmaceutical agent contains a compound in which a mucin-type sugar chain-binding lectin is bound to a polypeptide having an integrin recognition sequence.
    Type: Application
    Filed: May 24, 2010
    Publication date: April 7, 2011
    Applicant: THE DOSHISHA
    Inventors: Kimio NISHIMURA, Junji MORITA, Mayumi HIROSE, Toshiyuki MURAI
  • Publication number: 20110052514
    Abstract: The invention relates to cosmetic or therapeutic compositions that contain hydrolysed yeast proteins as an active ingredient, to the use of said cosmetic or therapeutic compositions, and to a method for cosmetic treatment.
    Type: Application
    Filed: February 11, 2009
    Publication date: March 3, 2011
    Applicant: LESAFFRE ET COMPAGNIE
    Inventors: Peter Jüsten, Dominique Marie Noelle Borreill, William Marques
  • Publication number: 20100306881
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: December 17, 2008
    Publication date: December 2, 2010
    Inventors: Paul Harris, Suchindra Maiyuran, Kimberly Brown
  • Publication number: 20100299789
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: December 17, 2008
    Publication date: November 25, 2010
    Applicant: Novozymes A/S
    Inventors: Paul Harris, Suchindra Maiyuran, Kimberly Brown
  • Publication number: 20100285173
    Abstract: The present invention relates to a glucose-induced inactivation/degradation resistant transporter gene and use thereof, and more particularly to a brewery yeast having excellent assimilability of oligosaccharides (maltose, maltotriose, etc.), an alcoholic beverage produced using the yeast, and so on. In particular, the present invention relates to a glucose-induced inactivation/degradation resistant transporter such as Mal21p, etc., a gene encoding the same, a method of producing an alcoholic beverage using the same; and so on.
    Type: Application
    Filed: September 9, 2008
    Publication date: November 11, 2010
    Applicant: SUNTORY HOLDINGS LIMITED
    Inventors: Haruyo Hatanaka, Fumihiko Omura
  • Patent number: 7820795
    Abstract: N-deoxyribosyl transferases of Lactobacillus fermentum and their analogues, as well their use for the enzymatic synthesis of 2?,3?-dideoxynucleosides and 2?,3?-didehydro-2?,3?-dideoxynucleosides. These transferases and their analogues include a N-deoxyribosyl transferase protein (DTP) that has at least 70%-95% identity with the polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, that retains residues Y13, D77, D97, E103, and M312 which respectively correspond to positions 13, 77, 97, 103, and 132 of SEQ ID NO: 2; and that has threonine at a position corresponding to position 15 of SEQ ID NO: 2 or SEQ ID NO: 4. Polynucleotides, vectors and host cells encoding these N-deoxyribosyl transferases and their analogues.
    Type: Grant
    Filed: March 29, 2005
    Date of Patent: October 26, 2010
    Assignee: Institut Pasteur
    Inventor: Pierre-Alexandre Kaminski
  • Publication number: 20100267019
    Abstract: The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptide toxins and toxin production in mushrooms. In particular, the present invention relates to using genes and proteins from Amanita species encoding Amanita peptides, specifically relating to amatoxins and phallotoxins. In a preferred embodiment, the present invention also relates to methods for detecting Amanita peptide toxin genes for identifying Amanita peptide-producing mushrooms and for diagnosing suspected cases of mushroom poisoning. Further, the present inventions relate to providing kits for diagnosing and monitoring suspected cases of mushroom poisoning in patients.
    Type: Application
    Filed: November 10, 2008
    Publication date: October 21, 2010
    Inventors: Heather E. Hallen, John S. Scott-Craig, Jonathan D. Walton, Hong Luo
  • Patent number: 7601808
    Abstract: An immunomodulatory protein is cloned from Ganoderma microsporum. Its molecular weight is 15863.79 Da. Its genome sequence and translated protein sequence are different from those protected by the known patent of glycoprotein LZ-8, which is isolated from the mycelium of G. lucidum and has immunomodulator effect, and its immunomodulator efficiency is better than that of LZ-8.
    Type: Grant
    Filed: March 2, 2006
    Date of Patent: October 13, 2009
    Assignee: World Bio-Tech Alliance Corporation
    Inventor: Tsai-Leng Lin
  • Publication number: 20090155239
    Abstract: An object of the present invention are to provide a protease that is stable in a wide pH range from acidic to alkaline, and that has excellent thrombolytic activity; a protease-producing microorganism that produces the above protease; and a process for producing the protease. By culturing a novel filamentous fungus belonging to the genus Fusarium (Fusarium sp. strain BLB), a protease that is stable in a wide pH range from acidic to alkaline and that has excellent thrombolytic activity, is formed and accumulated in the culture medium, and recovered.
    Type: Application
    Filed: March 22, 2006
    Publication date: June 18, 2009
    Applicant: SODX CO., LTD.
    Inventor: Takumi Nakamura
  • Patent number: 7507574
    Abstract: The present invention provides a modified Saccharomyces yeast which produces significantly lower levels of ethanol than wild-type yeast under aerobic conditions and saccharide concentrations of 2% glucose, and which exhibits a growth rate of at least 30% of the wild-type yeast, preferably containing a chimeric construct of at least 2 saccharide transporters, nucleic acid molecules encoding the chimeras and polypeptides encoded by such sequences, and methods of using the modified yeast for preparing products in the yeast.
    Type: Grant
    Filed: June 29, 2001
    Date of Patent: March 24, 2009
    Assignee: Gothia Yeast Solutions AB
    Inventors: Roslyn Bill, Eckhard Boles, Lena Gustafsson, Stefan Hohmann, Christer Larsson, Karin Elbing
  • Patent number: 7449443
    Abstract: The present invention provides a method for producing modified stable polypeptides introducing at least one non-natural amino acid into the hydrophobic region of the polypeptide. The thermal and chemical stability of such polypeptides is improved compared to those properties of its corresponding wild type proteins. The invention further provides purified leucine zipper and coiled-coil proteins in which the leucine residues have been replaced with 5,5,5-trifluoroleucines, and the modified proteins so produced demonstrate increased thermal and chemical stability compared to their corresponding wild-type natural proteins.
    Type: Grant
    Filed: May 21, 2004
    Date of Patent: November 11, 2008
    Assignee: California Institute of Technology
    Inventors: David A. Tirrell, Yi Tang
  • Publication number: 20080161225
    Abstract: The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.
    Type: Application
    Filed: December 27, 2006
    Publication date: July 3, 2008
    Inventors: Ching-Yu Tu, Li-Ling Liaw
  • Publication number: 20080145892
    Abstract: The present invention relates to the field of antibiotics, and more specifically to the isolation of nucleic acid molecules that code for the biosynthetic pathway of the glycopeptide antibiotic A40926. Disclosed are the functions of the gene products involved in A40926 production. The present invention provides biosynthetic genes that code for A40926 production, the encoded polypeptides, the recombinant vectors comprising the nucleic acid sequences that encode said polypeptides, the host cells transformed with said vectors and methods for producing glycopeptide antibiotics using said transformed host cells, including methods for producing A40926, a precursor thereof, a derivative thereof or a modified glycopeptide different from A 40926 or a precursor thereof.
    Type: Application
    Filed: October 15, 2003
    Publication date: June 19, 2008
    Applicant: Pfizer
    Inventors: Stefano Donadio, Margherita Sosio, Fabrizio Beltrametti
  • Patent number: 7361495
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: January 28, 2005
    Date of Patent: April 22, 2008
    Assignee: Novozymes, Inc.
    Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
  • Publication number: 20080069829
    Abstract: A substantially pure, isolated, antigenic protein from fungi of the genus Malassezia, characterized in that said antigenic protein has a binding ability to IgE antibodies from patients with allergoses; an antigenic fragment derived from the antigenic protein; and an antibody against the antigenic protein or fragments thereof. According to the present invention, there can be provided an isolated and purified antigenic protein having high purity from Malassezia, antigenic fragments thereof, and a specific antibody against those antigenic protein or fragments thereof. In addition, there can be provided a diagnostic agent, a therapeutic agent, or a prophylactic drug for Malassezia allergoses, wherein the agent includes, as an active ingredient, the antigenic protein or fragments thereof.
    Type: Application
    Filed: April 28, 2005
    Publication date: March 20, 2008
    Applicant: TAKARA BIO INC.
    Inventors: Kazutoh Takesako, Takashi Okado, Tomoko Yagihara, Masanobu Kuroda, Yoshimi Onishi, Ikunoshin Kato, Kazuo Akiyama, Hiroshi Yasueda, Hideyo Yamaguchi
  • Patent number: 7335757
    Abstract: According to the present invention, there is provided a novel carbonyl reductase derived from a microbial belonging to the genus Ogataea and a DNA encoding the enzyme. By reducing ketones with the use of the carbonyl reductase, optically active alcohols, in particular, (E)-7-[2-cyclopropyl-4-(4-fluorophenyl)-quinolin-3-yl]-3,5-dihydroxy-hept-6-enoic acid esters can be produced. The carbonyl reductase according to the present invention is excellent in activity and stereoselectivity. Thus, according to the present invention, there is provided a process for producing optically active alcohols, which are industrially useful as intermediate materials for drugs, pesticides, etc.
    Type: Grant
    Filed: September 17, 2004
    Date of Patent: February 26, 2008
    Assignees: Mitsubishi Chemical Corporation, Nissan Chemical Industries, Ltd.
    Inventors: Hirotoshi Hiraoka, Makoto Ueda, Mari Hara
  • Patent number: 7179595
    Abstract: The present invention provides non-single-chain antigen-binding units that is stabilized by leucine zipper sequences. The experimental design is particularly useful for generating and screening for Nsc Abus that remain the binding capabilities to their respective antigens within a cell. The present invention also provides recombinant polynucleotides, host cells and kits comprising the vectors. Further provided by the invention are methods of using the subject vectors.
    Type: Grant
    Filed: August 22, 2002
    Date of Patent: February 20, 2007
    Inventor: Shengfeng Li
  • Patent number: 7169899
    Abstract: A library of macrocyclic compounds of the formula (I) where part (A) is a ?bivalent radical, a —(CH2)y— bivalent radical or a covalent bond; where part (B) is a ?bivalent radical, a —(CH2)z— bivalent radical, or a covalent bond; where part (C) is a ?bivalent radical, a —(CH2)t— bivalent radical, or a covalent bond; and where part (T) is a —Y—L—Z— radical wherein number Y is CH2 or CO, Z is NH or O and L is a bivalent radical. These compounds are useful for carrying out screening assay or as intermediates for the synthesis of other compound of pharmaceutical interest. A process for their preparation of these compounds in a combinatorial manner, is also disclosed.
    Type: Grant
    Filed: October 4, 2000
    Date of Patent: January 30, 2007
    Assignee: Tranzyme Pharma Inc.
    Inventors: Pierre Deslongchamps, Yves Dory, Gilles Berthiaume, Luc Ouellet, Ruoxi Lan
  • Patent number: 6927026
    Abstract: The invention relates to an orotidine-5?-phosphate decarboxylase gene having the sequence of SEQ ID NO: 1 or its homologs, to a gene construct comprising this gene or its homologs, and to its use. The invention additionally relates to vectors or organisms comprising an orotidine-5?-phosphate decarboxylases gene having the sequence SEQ ID NO: 1 or its homologs. The invention further relates to a process for producing uracil-auxotrophic microorganisms and to a process for inserting DNA into uracil-auxotrophic microorganisms.
    Type: Grant
    Filed: December 18, 1998
    Date of Patent: August 9, 2005
    Assignee: BASF Aktiengesellschaft
    Inventors: Markus Pompejus, Jose Luis Revuelta Doval, Maria Angeles Santos Garcia
  • Patent number: 6903193
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells, isolated trichodiene synthases and nucleic acid sequences encoding the trichodiene synthases.
    Type: Grant
    Filed: November 10, 2000
    Date of Patent: June 7, 2005
    Assignee: Novozymes Biotech, Inc.
    Inventors: John C. Royer, Lynne M. Christianson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
  • Patent number: 6815169
    Abstract: The invention provides isolated nucleic acid and amino acid sequences of TL-&ggr;, antibodies to TL-&ggr;, methods of screening for TL-&ggr; modulators using biologically active TL-&ggr;, and kits for screening for TL-&ggr; modulators.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: November 9, 2004
    Assignee: The Regents of the University of California
    Inventors: Roman Sakowicz, Lawrence S. B. Goldstein