Binds Drug, Hapten, Hapten--carrier Complex, Or Specifically-identified Chemical Structure (e.g., Theophylline, Digoxin, Etc.) Patents (Class 530/388.9)
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Patent number: 7498421Abstract: A pharmaceutical composition comprising a compound of formula (I) wherein X is an electron withdrawing group, Y1 is hydrogen, alkyl, alkenyl, alkynyl, aryl, heterocyclyl, —SO2R4, —CO2R4, —CONHR4 or —COR4, and each of R1, R2 and R4, which may be the same or different, is hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, aryl or heterocyclyl, or a compound of formula (II) wherein each of Y2 and Y3, which may be the same or different, is hydrogen, alkyl, alkenyl, alkynyl, aryl, heterocyclyl, —SO2R9, —CO2R9, —CONHR9 or —COR9, Z is hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, aryl, heterocyclyl, —CH?C(NHR10)CH((CH2)m CO2R11)(C?O)CH3 or —CH2(C?O)CH((CH2)mCO2R11)(C?O)CH3, R8 is —(CH2)nCO2R12, each of R5 to R7 and R9 to R12, which may be the same or different, is hydrogen, alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, aryl or heterocyclyl, and each of m and n, which may be the same or different, is 1 to 6 or a compound of formula (III) wherein each of Y4 to Y6, which may be the same orType: GrantFiled: June 30, 2006Date of Patent: March 3, 2009Assignee: Medical Research CouncilInventors: Joseph Floyd Clark, Thomas Andrew Daniel Cadoux-Hudson, Christopher Joseph Schofield
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Publication number: 20090047289Abstract: Monoclonal antibodies immunospecific for osteopontin are disclosed. Also provided are therapeutic methods of use thereof for modulating osteopontin levels for the treatment of autoimmune disorders.Type: ApplicationFiled: August 6, 2008Publication date: February 19, 2009Inventors: David T. Denhardt, Lawrence Stainman
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Publication number: 20090036653Abstract: The instant invention comprises a process for selecting and manufacturing antibodies useful for therapeutic, prophylactic, diagnostic or research purposes using epitope peptide mixtures synthesized by the solid phase synthesis, such process defined by a set of rules regarding the identity and the frequency of occurrence of amino acids that substitute a base or native amino acid of a known epitope. The resulting antibodies are related to but distinct from antibodies that bind to the known epitope.Type: ApplicationFiled: May 7, 2008Publication date: February 5, 2009Applicant: Peptimmune, Inc.Inventor: Dustan Bonnin
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Patent number: 7482437Abstract: Activated haptens useful for generating immunogens to HIV protease inhibitors, immunogens useful for producing antibodies to HIV protease inhibitors, and antibodies and labeled conjugates useful in immunoassays for the HIV protease inhibitor saquinavir. The novel haptens feature an activated functionality at the central, non-terminal hydroxyl group. Also described are monoclonal antibodies specific for saquinavir having less than 10% cross-reactivity with lopinavir, nelfinavir, amprenavir, ritonavir, and indinavir, and a murine hybridoma producing said antibodies.Type: GrantFiled: February 2, 2007Date of Patent: January 27, 2009Assignee: Roche Diagnostics Operations, Inc.Inventors: Gerald F. Sigler, Raymond A. Hui, Ina Deras, Richard Terry Root, Mitali Ghoshal, Erasmus Huber, Herbert W. Von Der Eltz, Sigrun Metz, Peter Kern
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Publication number: 20080318227Abstract: The invention provides a protein in a form that is functional for the enzymatic conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate to 1-hydroxy-2-methyl-2-butenyl 4-diphosphate notably in its (E)-form of the non-mevalonate biosynthetic pathway to isoprenoids. The invention also provides a protein in a form that is functional for the enzymatic conversion of 1-hydroxy-2-methyl-2-butenyl 4-diphosphate, notably in its (E)-form, to isopentenyl diphosphate and/or dimethylallyl diphosphate. Further, screening methods for inhibitors of these proteins are provided. Further, 1-hydroxy-2-methyl-2-butenyl 4-diphosphate is provided and chemical and enzymatic methods of its preparation.Type: ApplicationFiled: August 30, 2007Publication date: December 25, 2008Inventors: Adelbert Bacher, Felix Rohdich, Petra Adam, Sabine Amslinger, Wolfgang Eisenreich, Stefan Hecht
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Patent number: 7459281Abstract: Novel conjugates of docetaxel and novel docetaxel immunogens derived from the 7 and 10 positions of docetaxel and monoclonal antibodies generated by these docetaxel linked immunogens are useful in immunoassays for the quantification and monitoring of docetaxel in biological fluids.Type: GrantFiled: March 20, 2006Date of Patent: December 2, 2008Assignee: Saladax Biomedical Inc.Inventors: Salvatore J. Salamone, Jodi Blake Courtney, Gregory Drake Lundell, Mahmoud Ahmed ElSohly, Waseem Gul
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Publication number: 20080287658Abstract: Novel conjugates of docetaxel and novel docetaxel immunogens derived from the 7 and 10 positions of docetaxel and monoclonal antibodies generated by these docetaxel linked immunogens are useful in immunoassays for the quantification and monitoring of docetaxel in biological fluids.Type: ApplicationFiled: July 14, 2008Publication date: November 20, 2008Inventors: Salvatore J. Salamone, Jodi Blake Courtney, Gregory Drake Lundell, Mahmoud Ahmed ElSohly, Waseem Gul
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Publication number: 20080275221Abstract: Novel conjugates of busulfan and novel busulfan immunogens derived from ?-substituted derivatives of busulfan and antibodies generated by these busulfan linked immunogens are useful in immunoassays for the quantification and monitoring of busulfan in biological fluids.Type: ApplicationFiled: July 9, 2008Publication date: November 6, 2008Inventors: Salvatore J. Salamone, Jodi Blake Courtney, Shu He
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Publication number: 20080226650Abstract: The present application discloses monoclonal antibody against cotinine and nicotine.Type: ApplicationFiled: March 14, 2008Publication date: September 18, 2008Inventors: Sunyoung PARK, Junho CHUNG, Dohoon LEE, Jae-Gahb PARK
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Publication number: 20080227959Abstract: The invention provides derivatives of efavirenz and methods of making derivatives of efavirenz. The derivatives include immunogenic compounds for producing antibodies to efavirenz and labeled efavirenz tracers. These compounds are useful in immunoassay methods for determining efavirenz.Type: ApplicationFiled: May 16, 2008Publication date: September 18, 2008Inventors: Mitali Ghoshal, Gerald Sigler, Anlong Ouyang, Richard Root
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Publication number: 20080199887Abstract: Compounds and methods for use in detecting gabapentin in a sample suspected of containing gabapentin are disclosed. Gabapentin derivatives are used to produce gabapentin conjugates. A gabapentin-immunogenic carrier conjugate may be used as an immunogen for the preparation of an anti-gabapentin antibody. A gabapentin-detectable label may be used in a signal producing system in gabapentin assays.Type: ApplicationFiled: February 15, 2008Publication date: August 21, 2008Inventors: Johnny Jose Valdez, Byung Sook Moon, Michael Kevin Helms, Alejandro A. Orozco
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Publication number: 20080124284Abstract: The present invention relates to an oral composition and an immunogenic composition for the suppression of the pathogenic effects of the intra-oral bacterium Porphyromonas gingivalis associated with periodontal disease.Type: ApplicationFiled: March 30, 2007Publication date: May 29, 2008Inventors: Eric Charles Reynolds, Neil Martin O'Brien-Simpson, Nada Slakeski
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Patent number: 7355010Abstract: The present invention relates to methods for detecting, quantifying and high throughput screening of donor-products and the catalytic activities generating the donor-products in group-transfer reactions. The invention further provides immunoassays, antibodies and kits that may be used to practice the methods of the invention.Type: GrantFiled: May 26, 2005Date of Patent: April 8, 2008Assignee: BellBrook Labs, LLCInventors: Robert Lowery, Karen Kleman-Leyer, Matt Staeben, Thane Westermeyer
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Publication number: 20080050376Abstract: The present invention discloses antibodies, antigen binding fragments, peptides and peptidomimetics immunoreactive with provasopressin and compositions thereof, methods of phenotyping tissue samples, methods of treating cancer, and kits for phenotyping test biopsy samples and bodily fluids for breast cancer, small cell lung cancer, ductal carcinoma in situ, and atypical ductal hyperplasia.Type: ApplicationFiled: July 16, 2003Publication date: February 28, 2008Inventors: William G. North, Brendan P. Keegan, Lyn Oligino
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Patent number: 7335749Abstract: The invention provides isolated nucleic acid and amino acid sequences of novel human tumor suppressors, antibodies to such tumor suppressors, methods of detecting such nucleic acids and proteins, methods of screening for modulators of tumor suppressors, and methods of diagnosing and treating tumors with such nucleic acids and proteins.Type: GrantFiled: February 9, 2001Date of Patent: February 26, 2008Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Curtis C. Harris, Makoto Nagashima
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Patent number: 7279561Abstract: Provided are rapamycin conjugates which are useful as immunogenic molecules for the generation of antibodies specific for rapamycin, for measuring levels of rapamycin or derivatives thereof; for isolating rapamycin binding proteins; and detecting antibodies specific for rapamycin or derivatives thereof. This invention also provides a rapamycin specific monclonal antibody.Type: GrantFiled: May 24, 2000Date of Patent: October 9, 2007Assignee: WyethInventors: Katherine L. Molnar-Kimber, Craig E. Caufield, Timothy D. Ocain
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Patent number: 7276347Abstract: Novel conjugates of protected aldehyde active metabolites of cyclophosphamide including reagents and immunogens thereof and monoclonal antibodies generated by these immunogens, said reagents and immunogens useful in immunoassays for the monitoring of the active metabolites of cyclophosphamide in patients being treated with cyclophosphamide.Type: GrantFiled: July 20, 2005Date of Patent: October 2, 2007Assignee: Saladax Biomedical Inc.Inventors: Salvatore J. Salamone, Jodi Blake Courtney, Dennis Stocker
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Patent number: 7271252Abstract: The invention provides derivatives of efavirenz and methods of making derivatives of efavirenz. The derivatives include immunogenic compounds for producing antibodies to efavirenz and labeled efavirenz tracers. These compounds are useful in immunoassay methods for determining efavirenz.Type: GrantFiled: April 22, 2003Date of Patent: September 18, 2007Assignee: Roche Diagnostics Operations, Inc.Inventors: Gerald F. Sigler, Mitali Ghoshal, Lili Arabshahi
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Patent number: 7229782Abstract: The present invention is directed antibodies specific to multiple beta blockers, as well as immunogens used to produce the antibodies and immunoassay kits and methods for using the antibodies.Type: GrantFiled: August 3, 2004Date of Patent: June 12, 2007Assignee: LabOne, Inc.Inventor: Liuming Yu
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Patent number: 7230085Abstract: The present invention relates to an antibody or antibody fragment that binds to 1,4,7,10-tetrazacyclododecane-N,N?,N?,N??-tetraacetic acid (DOTA),which is bound to an alkyl-amino group through one of its pendant carb.oxyl groups.Type: GrantFiled: November 27, 2002Date of Patent: June 12, 2007Assignee: Immunomedics, Inc.Inventors: Gary L. Griffiths, Hans J. Hansen, Serengulam V. Govindan, Michelle Losman, Zhengxing Qu
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Patent number: 7223553Abstract: Immunoassays for the detection of everolimus are provided. Compounds for producing antibodies for everolimus, as well as antibodies produced therefrom, are also provided.Type: GrantFiled: February 13, 2006Date of Patent: May 29, 2007Inventors: Mark Roberts, Lili Arabshahi, Jared Boyd, Christopher T. Dennis, Peter Marbach, George Aaron, Deng Hwang, Alexei Boris Shvets
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Patent number: 7220842Abstract: Methods, compositions and kits are disclosed directed at haptens, immunogens and immunoassays for buprenorphine (BUP) and nor buprenorphine (norBUP). The method comprises providing in combination in a medium (i) a sample suspected of containing buprenorphine (BUP) or norbuprenorphine (norBUP) and (ii) an antibody raised against an immunogen of buprenorphine (BUP) or norbuprenorphine (norBUP). The medium is examined for the presence of a complex comprising a labeled hapten of buprenorphine (BUP) or norbuprenorphine (norBUP) where the presence of such as complex indicates the presence of the compound in the sample.Type: GrantFiled: April 5, 2004Date of Patent: May 22, 2007Assignee: Dade Behring Inc.Inventors: Yi-Feng Zheng, Pratap Singh, Dorota Bolle, Hshiou-Ting Liu
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Patent number: 7217802Abstract: The invention provides a hapten, an immunogen comprising the hapten coupled to an antigenicity-conferring carrier material, a conjugate comprising the hapten coupled to a labelling agent, as well as, antibodies raised against the aforementioned immunogen and capable of binding with MDMA.Type: GrantFiled: December 20, 2002Date of Patent: May 15, 2007Assignee: Randox Laboratories LimitedInventors: Robert Ivan McConnell, Elouard Benchikh, Stephen Peter Fitzgerald, John Victor Lamont
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Patent number: 7202348Abstract: The present invention provides synthetic immunochemical haptens for the generation of antibodies that are designed to recognize the common molecular features of d-methamphetamine-like abused stimulants with insignificant cross-reactivity to endogenous substrates (e.g. dopamine) or over-the-counter medications (e.g. 1-methamphetamine, pseudoephedrine, phenylpropanolamine and ephedrine). These monoclonal antibodies and their antigen binding fragments are useful in treatment plans for recovering addicts, in emergency room settings for rapidly reversing a drug overdose, in protection of fetuses from drug-abusing pregnant mothers or in a psychiatric setting to reduce the exacerbation of psychotic disorders caused by stimulant drugs.Type: GrantFiled: September 26, 2002Date of Patent: April 10, 2007Assignee: The University of Arkansas for Medical SciencesInventors: Samuel M. Owens, Frank Ivy Carroll, Philip Abraham
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Patent number: 7202041Abstract: The present invention relates to an immunoreaction measuring method for measuring an antigen or antibody contained as a subject substance in a sample, wherein the sample was mixed with at least one compound selected from the group consisting of dicarboxylic acids having a hydroxyl group, dicarboxylic acids having a double bond, straight-chain dicarboxylic acids expressed by the chemical formula (1): HOOC(CH2)nCOOH (n is an integer), and the salts of these dicarboxylic acids, and an antibody or antigen as a specifically binding substance capable of specifically binding to the subject substance, to obtain an acidic reaction solution, and then an antigen-antibody complex, generated by an antigen-antibody reaction of the subject substance with the specifically binding substance in the reaction solution was detected. This makes it possible to improve a measurement value and to relax a limitation of a measurement range due to a zone phenomenon that occurs in an antigen-excess region.Type: GrantFiled: December 9, 2003Date of Patent: April 10, 2007Assignee: Matsushita Electric Industrial Co., Ltd.Inventors: Akihito Kamei, Tatsurou Kawamura, Keiko Yugawa
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Patent number: 7195882Abstract: The present invention relates to monoclonal antibodies that specifically bind buprenorphine and/or at least one metabolic product thereof. The present invention further relates to buprenorphine metabolite conjugates for the production of monoclonal antibodies that specifically bind buprenorphine and/or at least one metabolic product thereof and hybridoma cells that produce the monoclonal antibodies. The invention also relates to immunoassay methods for determining buprenorphine and/or one or more buprenorphine metabolites in a sample using the novel antibodies and conjugates of the present invention.Type: GrantFiled: June 3, 2003Date of Patent: March 27, 2007Assignee: Roche Diagnostics Operations, Inc.Inventors: Richard T. Root, Mitali Ghoshal
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Patent number: 7193065Abstract: Activated haptens useful for generating immunogens to HIV protease inhibitors, immunogens useful for producing antibodies to HIV protease inhibitors, and antibodies and labeled conjugates useful in immunoassays for HIV protease inhibitors. The novel haptens feature an activated functionality at the central, non-terminal hydroxyl group common to all HIV protease inhibitors, e.g., saquinavir, nelfinavir, indinavir, amprenavir, ritonavir, lopinavir, and atazanavir.Type: GrantFiled: September 24, 2003Date of Patent: March 20, 2007Assignee: Roche Diagnostics Operations, Inc.Inventors: Gerald F. Sigler, Raymond A. Hui, Ina Deras, Richard Terry Root, Mitali Ghoshal, Erasmus Huber, Herbert W. Von Der Eltz, Sigrun Metz, Peter Kern
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Patent number: 7192722Abstract: The present invention relates to a method for preparing haptens of formulae I and II that are useful in the preparation of immunogens, antibodies and conjugates, for use in competitive immunoassays for the detection of ractopamine, isoxsuprine and ritodrine. The haptens are prepared by reacting a phenylethanolamine derivative of formula D with a phenylalkylcarbonyl derivative of formula E: in which, in formula I, Z1 is a crosslinker and Z2 is H and, in formula II, Z1 is H and Z2 is a crosslinker.Type: GrantFiled: November 10, 2005Date of Patent: March 20, 2007Assignee: Randox LaboratoriesInventors: Robert Ivan McConnell, Stephen Peter Fitzgerald, El Ouard Benchikh, Andrew Philip Lowry
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Patent number: 7175993Abstract: Novel conjugates of taxol and novel taxol immunogens derived from the 9 and 7 positions of taxol and monoclonal antibodies generated by these taxol linked immunogens are useful in immunoassays for the quantification and monitoring of taxol in biological fluids.Type: GrantFiled: December 12, 2005Date of Patent: February 13, 2007Assignee: Saladax Biomedical, Inc.Inventors: Salvatore J. Salamone, Jodi Blake Courtney, Dennis Stocker, Mahmoud Ahmed ElSohly, Waseem Gul
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Patent number: 7169907Abstract: Compounds including haptens, intermediates, and immunogens that are useful in the production of antibodies specific for the methylenedioxy class of amphetamine derivatives are described. Antibodies specific for the methylenedioxy class of amphetamine derivatives, reagent kits containing antibodies specific for the methylenedioxy class of amphetamine derivatives, methods of producing antibodies specific for the methylenedioxy class of amphetamine derivatives, and methods of detecting analytes including members of the methylenedioxy class of amphetamine derivatives are also described.Type: GrantFiled: March 1, 2002Date of Patent: January 30, 2007Assignee: Roche Diagnostics Operations, Inc.Inventor: Raymond A. Hui
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Patent number: 7157560Abstract: The present invention provides hapten derivatives useful for the preparation of antigens, antibodies and reagents for use in immunoassays for the detection of LSD and 2-oxo-3-hydroxy LSD. In the present invention, the 2-oxy LSD nucleus is derivatized out of the indole nitrogen to form an aminoalkyl derivative. The resulting haptens can then be further modified at this functionalized position for linking to appropriate immunogenic or labeling groups to provide reagents for immunoassays having substantially equal specificity for both LSD and 2-oxo-3-hydroxy-LSD.Type: GrantFiled: July 18, 2002Date of Patent: January 2, 2007Assignee: Roche Diagnostics Operations, Inc.Inventors: Mitali Ghoshal, Stephen Vitone, Gerald F. Sigler, Alan J. McNally
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Patent number: 7157561Abstract: Activated haptens useful for generating immunogens to the HIV protease inhibitor atazanavir, immunogens useful for producing antibodies to atazanavir, and antibodies and labeled conjugates useful in immunoassays for determination of atazanavir. The haptens feature an activated functionality at the central, non-terminal hydroxyl group.Type: GrantFiled: November 5, 2004Date of Patent: January 2, 2007Assignee: Roche Diagnostics Operations, Inc.Inventors: Raymond A. Hui, Richard T. Root
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Patent number: 7148029Abstract: The present invention provides immunogens for generating anti-neonicotinoid antibodies as well as antibodies, methods, reagents and kits for determining the presence of one or more neonicotinoid insecticides in a sample by immunoassay. The present invention has particular application to determination of the concentration of neonicotinoid insecticides applied to a plant propagation materials such as seed.Type: GrantFiled: December 6, 2000Date of Patent: December 12, 2006Assignee: Syngenta Crop Protection, Inc.Inventors: James Francis Brady, Dana Philip Simmons, Timothy Edward Wilson
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Patent number: 7138504Abstract: The invention teaches derivatives of mycophenolic alcohol and methods of preparing immunogens and other conjugates useful in immunoassays for quantitatively measuring concentrations of mycophenolic acid (MPA) and/or active metabolites of MPA in patient specimens. Antibodies produced from the disclosed immunogens capable of binding to MPA with cross-reactivity of no more than 5% with inactive metabolites and commonly co-prescribed drugs. Further, immunoassays for measuring the concentration of MPA using such antibodies are taught.Type: GrantFiled: August 11, 2005Date of Patent: November 21, 2006Assignee: Microgenics CorporationInventors: Vani B. Bodepudi, Amy P. Tsai, Weixing Luo, Rueyming Loor
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Patent number: 7122184Abstract: The invention concerns momoclonal antibodies to terbinafine in free base or salt form, immunogenic conjugates suitable for preparing them, hybridoma capable of producing them, and corresponding assay kits. The antibodies can be prepared by administering to an appropriate animal an immunogenic conjugate of a suitable derivative of terbinafine covalently linked to an immonogen, recovering antibody-producing cells sensitized to the conjugate, immortalizing the antibody-producing cells, selecting a resultant immortalized cell line, and recovering the resultant antibodies therefrom. They are indicated for use in particular in the measurement of terbinafine tissue concentration and distribution in bodily fluids or compartments, especially nails.Type: GrantFiled: February 12, 2001Date of Patent: October 17, 2006Assignee: Novartis AGInventors: Birgit Dorobek, Peter Nussbaumer
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Patent number: 7115718Abstract: The invention provides a hapten derivatized with a crosslinker at the nitrogen of the 8?-carboxamide of 2-oxo-3-hydroxy LSD. The invention also provides an immunogen comprising the aforementioned hapten coupled to an antigenicity-conferring carrier material; a conjugate comprising the aforementioned hapten coupled to a labelling agent, as well as, antibodies raised against the aforementioned immunogen and capable of binding with at least the 3-hydroxy-2-pyrrolidone structural epitope of 2-oxo-3-hydroxy LSD.Type: GrantFiled: December 20, 2002Date of Patent: October 3, 2006Assignee: Randox :abpratproes :o, otedInventors: Robert Ivan McConnell, Elouard Benchikh, Stephen Peter Fitzgerald, John Victor Lamont
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Patent number: 7115383Abstract: Methods, compositions and kits are disclosed. The compounds disclosed comprise an amphetamine moiety and a methamphetamine moiety linked together by a first linking group. A second linking group depends from the first linking group and comprises a functional group. The distance of the amphetamine moiety and the methamphetamine moiety from the point of linkage of the second linking group to the first linking group is approximately the same. The compounds may be linked to labels and used in assays for the detection of amphetamine and/or methamphetamine in samples suspected of containing these drugs.Type: GrantFiled: March 22, 2004Date of Patent: October 3, 2006Assignee: Dade Behring Inc.Inventors: Yi Feng Zheng, Richard F. Parrish, Johnny Valdez, Hshiou-ting Liu
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Patent number: 7109310Abstract: The invention provides an immunogen comprising a hapten coupled to an antigenicity-conferring carrier material, a conjugate comprising the aforementioned hapten coupled to a labelling agent, as well as, antibodies raised against the aforementioned immunogen and capable of binding with at least one structural epitope of metabolites of fentanyl and of metabolites of fentanyl analogs.Type: GrantFiled: November 15, 2002Date of Patent: September 19, 2006Assignee: Randox Laboratories, Ltd.Inventors: Robert Ivan McConnell, Elouard Benchikh, Stephen Peter Fitzgerald, John Victor Lamont
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Patent number: 7108992Abstract: The present invention provides methods for detecting activation of ATM kinase, DNA damage, and DNA damaging agents. Further provided are antibodies which specifically recognize the phosphorylation state of Ataxia Telangiectasia-Mutated (ATM) kinase. Methods of identifying agents which modulate the activation and activity of ATM kinase are also provided.Type: GrantFiled: November 27, 2002Date of Patent: September 19, 2006Assignee: St. Jude Children's Research HospitalInventors: Michael B. Kastan, Christopher Bakkenist
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Patent number: 7105643Abstract: A monoclonal antibody, and a cell line capable of producing the same, has been produced with the ability to detect the primary metabolites generated from the pyrolysis of smokeable, or “crack”, cocaine. This monoclonal antibody, while being highly specific for anhydroecgonine methyl ester (AEME) and ecgonidine (ECD), does not cross-react at a significant level with the primary cocaine metabolites of powdered or injected cocaine. Also, crack cocaine conjugates capable of evoking an immune response in animals have been produced.Type: GrantFiled: July 30, 2003Date of Patent: September 12, 2006Assignee: The United States of America as represented by the Attorney General of the Dept. of JusticeInventor: Natalie T. Lu
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Patent number: 7101980Abstract: Compounds including haptens, intermediates, and immunogens that are useful in the production of antibodies specific for the methylenedioxy class of amphetamine derivatives are described. Antibodies specific for the methylenedioxy class of amphetamine derivatives, reagent kits containing antibodies specific for the methylenedioxy class of amphetamine derivatives, methods of producing antibodies specific for the methylenedioxy class of amphetamine derivatives, and methods of detecting analytes including members of the methylenedioxy class of amphetamine derivatives are also described.Type: GrantFiled: July 18, 2003Date of Patent: September 5, 2006Assignee: Roche Diagnostics Operations, Inc.Inventors: Raymond A. Hui, Stephen Vitone, Richard Terry Root, Irina Baburina, Sheri Jordan
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Patent number: 7078495Abstract: An IgG1 ? monoclonal antibody to the immunosuppressive drug tacrolimus has improved properties. In particular, this monoclonal antibody, designated 1H6, has reduced cross-reactivity to several tacrolimus metabolites. This antibody is suitable for performance of immunoassays such as homogeneous immunoassays to detect or determine the presence or concentration of tacrolimus in samples such as blood samples. The invention further includes derivatives of tacrolimus derivatized at a non-binding portion of the molecule useful in immunizing antibody-producing animals and in producing such monoclonal antibodies, as well as labeled derivatives of tacrolimus useful as tacrolimus analogues in such assays. The invention further includes immunoassay methods for the detection of tacrolimus and test kits useful in performing such immunoassays.Type: GrantFiled: August 3, 1999Date of Patent: July 18, 2006Assignee: Dade Behring Inc.Inventors: Kenneth Cornell Kasper, Henry Jeong, Dariush Davalian, Hshiou-ting Liu, Paul Levi Miller, Denise Leah Williams
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Patent number: 7067270Abstract: An object of the present invention is to provide a method for detecting a protein using a nonradioactive label which is prevented to a smaller extent than in the case of the interaction of biotin/(strepto)avidin, and which achieves higher detection sensitivity than that of the detection system using DIG. The present invention provides a method for detecting a target substance, which comprises steps of: (A) allowing a target substance to come into contact with a protein labeled with a compound having a 6-membered ring, so as to carry out a binding reaction; and (B) detecting the protein labeled with the compound having a 6-membered ring, which was bound to the target substance, by using an antibody against the above compound having a 6-membered ring.Type: GrantFiled: December 13, 2004Date of Patent: June 27, 2006Assignee: Fuji Photo Film Co., Ltd.Inventor: Yukio Sudo
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Patent number: 7038021Abstract: The object of present invention is to provide an immunoassay for dioxins which can rapidly and simply afford measured values having a good correlation with analytical values of dioxins by the official method (GC/MS method). The above object is achieved by using the monoclonal antibody of the present invention having not only a reactivity with the indicator isomer among 17 kinds of PCDDs and PCDFs each having a predetermined WHO-TEF value, but also a high cross-reactivity with several kinds of dioxin isomers having five or six chlorine atoms which contribute largely to a TEQ value, and also having a stable reactivity with the antigens in a measuring solvent.Type: GrantFiled: July 7, 2004Date of Patent: May 2, 2006Assignee: Kyoto Electronics Manufacturing Co., Ltd.Inventors: Yoko Takagi, Kazuyuki Sawadaishi, Chiwa Kataoka
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Patent number: 7037669Abstract: Methods, compositions and kits are disclosed. Enzyme conjugates of Formula I may be employed in assays for the determination of an amphetamine and/or a methamphetamine. Immunogenic conjugates of Formula I may be employed to prepare antibodies for an amphetamine and/or for a methamphetamine for use in assays for the determination of an amphetamine and/or a methamphetamine. The enzyme conjugates may also be employed to screen antibodies for use in such methods.Type: GrantFiled: March 22, 2004Date of Patent: May 2, 2006Assignee: Dade Behring Inc.Inventors: Yi Feng Zheng, Khaled A. Yamout, Donald E. Berger, Jr., Mae W. Hu, Hshiou-ting Liu
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Patent number: 7026134Abstract: Novel haptens, which can be conjugated to form immunogens, are of formula I, II or III wherein R is a divalent alkyl, cycloalkyl or aryl group having 1 to 10 carbon atoms, and X is a functional group.Type: GrantFiled: December 20, 2002Date of Patent: April 11, 2006Assignee: Randox Laboratories Ltd.Inventors: John Victor Lamont, Robert Ivan McConnell, Stephen Peter Fitzgerald, El Ouard Benchikh, Andrew Philip Lowry
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Patent number: 7022492Abstract: Methods, compositions and kits are disclosed. Enzyme conjugates of Formula I are employed in assays for the determination of an methylenedioxyamphetamine, a methylene-dioxyethamphetamine, and/or a methylenedioxymethamphetamine. Immunogenic conjugates of Formula I are employed to prepare antibodies for an methylenedioxyamphetamine, a methylenedioxyethamphetamine, and/or for a methylene-dioxymethamphetamine for use in assays for the determination of an methylenedioxyamphetamine, a methylenedioxyethamphetamine, and/or a methylene-dioxymethamphetamine. The enzyme conjugates may also be employed to screen antibodies for use in such methods.Type: GrantFiled: December 15, 2003Date of Patent: April 4, 2006Assignee: Dade Behring Inc.Inventors: Yi Feng Zheng, Yali Yang
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Patent number: 6991911Abstract: Methods, compositions and kits are disclosed. The methods are directed to determining the presence of entactogen analytes such as, for example, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-methamphetamine (MDMA), 3,4-methylenedioxy-ethylamphetamine (MDEA) and 4-hydroxy-3-methoxy-methamphetamine (HMMA). The method comprises providing in combination in a medium (i) a sample suspected of containing the compound and (ii) an antibody raised against a compound of Formula I that comprises a protein. The medium is examined for the presence a complex comprising the compound and the antibody where the presence of such as complex indicates the presence of the compound in the sample. In one aspect of the above embodiment, the combination further comprises a label conjugate of the compound Formula I.Type: GrantFiled: December 15, 2003Date of Patent: January 31, 2006Assignee: Dade Behring Inc.Inventors: Yi Feng Zheng, Hshiou-ting Liu
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Patent number: 6992177Abstract: Analogs of saquinavir functionalized at the quinoline portion of the molecule are described. These include pyridyl analogs (replacing the quinoline ring) with a functional handle out of the ring allowing for elaboration with linkers terminated by a functional group such as an activated ester which are useful for attaching the molecule to other entities such as proteins, polysaccharides, and the like. Analogs of saquinavir derivatized out of the quinoline ring are also described.Type: GrantFiled: December 10, 2004Date of Patent: January 31, 2006Assignee: Roche Diagnostics Operations, Inc.Inventors: Raymond A. Hui, Gerald F. Sigler, Richard T. Root, Wei Yuan
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Patent number: RE40596Abstract: Monoclonal antibodies to rapamycin and to 40-O-alkylated derivatives of rapamycin are provided, together with novel haptens, immunogenic conjugates, and processes for making them and assay kits for using them.Type: GrantFiled: February 6, 2007Date of Patent: December 2, 2008Assignee: Novartis AGInventors: Richard Sedrani, Valerie Quesniaux Ryffel