Sulfur Containing Reactant Patents (Class 530/408)
  • Publication number: 20150125892
    Abstract: The invention relates to fused cyclooctyne compounds, and to a method for their preparation. The invention also relates to a conjugate wherein a fused cyclooctyne compound according to the invention is conjugated to a label, and to the use of these conjugates in bioorthogonal labeling, imaging and/or modification, such as for example surface modification, of a target molecule. The invention further relates to a method for the modification of a target molecule, wherein a conjugate according to the invention is reacted with a compound comprising a 1,3-dipole or a 1,3-(hetero)diene.
    Type: Application
    Filed: October 10, 2014
    Publication date: May 7, 2015
    Applicant: SynAffix B.V.
    Inventors: Floris Louis VAN DELFT, Floris Petrus Johannes Theodorus Rutjes, Frederik Jan Dommerholt
  • Patent number: 9023653
    Abstract: A method of labeling a sulfenic acid (—SOH) group of a cysteine residue in a protein; or peptide, comprises contacting said protein or peptide with a beta-ketoester to covalently couple said beta-ketoester to said cysteine residue and form a beta-ketoester-labeled cysteine residue in said protein or peptide.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: May 5, 2015
    Assignee: Wake Forest University Health Sciences
    Inventors: Cristina M. Furdui, Allen W. Tsang, Jiang Qian
  • Publication number: 20150119553
    Abstract: Compounds are disclosed that are useful for noninvasive imaging in the near-infrared spectral range.
    Type: Application
    Filed: November 24, 2014
    Publication date: April 30, 2015
    Inventors: Xinshe Xu, Daniel R. Draney, Lael Cheung
  • Patent number: 9005910
    Abstract: The purpose is to produce, with high reproducibility, a complex of labeled probes and a carrier, said complex being to be used for detecting and measuring a target substance to be measured with high sensitivity and high stability. The means for accomplishing the purpose is that a label is bound to a probe-water soluble carrier conjugate using specific binding of an avidin compound such as avidin, streptavidin, etc. to biotin, and the binding of the avidin compound to the probe is performed before the binding to the carrier. Namely, after conjugating the avidin compound to a substance which is capable of binding to the target substance, the conjugate is bound to a high-molecule water-soluble carrier to produce a complex of the avidinized probes and the water-soluble carrier. Then the complex of the avidinized probes and the water-soluble carrier is mixed with a biotinylated label.
    Type: Grant
    Filed: April 13, 2011
    Date of Patent: April 14, 2015
    Assignees: Eiken Kagaku Kabushiki Kaisha, Advanced Life Science Institute, Inc.
    Inventors: Yoshiyuki Ohiro, Susumu Takayasu
  • Patent number: 9005926
    Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
    Type: Grant
    Filed: October 1, 2010
    Date of Patent: April 14, 2015
    Assignee: Biogen Idec Ma Inc.
    Inventors: David Evans, R. Blake Pepinsky, Dingyi Wen, Rashmi Rohit Kshirsagar, Karin Lucas
  • Publication number: 20150093370
    Abstract: The present invention provides albumin-binding probes capable of reversibly linking to short-lived amino-containing drugs and non-covalently associating with albumin in-vivo, thereby converting said drugs into inactive reactivable prodrugs having prolonged lifetime in-vivo. The invention further provides conjugates of said probes with amino-containing drugs, as well as pharmaceutical compositions and uses thereof.
    Type: Application
    Filed: December 9, 2014
    Publication date: April 2, 2015
    Inventors: Yoram SHECHTER, Matityahu FRIDKIN
  • Patent number: 8993350
    Abstract: The present invention relates generally to a novel method using HPLC and fluorescence detection of free PEG-mal in PEGylated proteins and PEG-mal raw materials by adding a fluorescent label to the free PEG-mal.
    Type: Grant
    Filed: January 29, 2010
    Date of Patent: March 31, 2015
    Assignee: Bristol-Myers Squibb Company
    Inventors: Mei Lin, Anulfo Valdez
  • Publication number: 20150068904
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.
    Type: Application
    Filed: March 15, 2013
    Publication date: March 12, 2015
    Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED
    Inventors: Mark Bruce, James Clarke, Andrew Heron, Lakmal Jayasinghe, Jayne Wallace
  • Publication number: 20150038686
    Abstract: The present invention concerns methods and compositions for delivery of therapeutic agents to target cells, tissues or organisms. In preferred embodiments, the therapeutic agents are delivered in the form of therapeutic-loaded polymers that may comprise many copies of one or more therapeutic agents. In more preferred embodiments, the polymer may be conjugated to a peptide moiety that contains one or more haptens, such as HSG. The agent-polymer-peptide complex may be delivered to target cells by, for example, a pre-targeting technique utilizing bispecific or multispecific antibodies or fragments, having at least one binding arm that recognizes the hapten and at least a second binding arm that binds specifically to a disease or pathogen associated antigen, such as a tumor associated antigen. Methods for synthesizing and using such therapeutic-loaded polymers and their conjugates are provided.
    Type: Application
    Filed: October 9, 2014
    Publication date: February 5, 2015
    Inventors: Serengulam V. Govindan, Sung-Ju Moon, David M. Goldenberg, Chien-Hsing Chang
  • Patent number: 8946392
    Abstract: The present invention comprises novel conjugates and immunogens derived from gemcitabine and unique antibodies generated by using gemcitabine linked immunogens, which conjugates immunogens and antibodies, are useful in immunoassays for the quantification and monitoring of gemcitabine in biological fluids.
    Type: Grant
    Filed: June 14, 2011
    Date of Patent: February 3, 2015
    Assignee: Saladax Biomedical Inc.
    Inventors: Salvatore J. Salamone, Jodi Blake Courtney, Howard Sard, Christopher Spedaliere
  • Publication number: 20150011433
    Abstract: Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.
    Type: Application
    Filed: June 12, 2014
    Publication date: January 8, 2015
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Erik Miller, Satwik Kamtekar, Keith Bjornson, Jeremiah Hanes
  • Patent number: 8927291
    Abstract: Disclosed are dithio compounds that include a quenched fluorophore and a non-fluorophore peptide linked via a dithio bond to the fluorophore. The dithio compounds may be used in methods for detecting thiol-containing compounds or dithio-containing compounds. The dithio compounds also may be used as cellular probes where the peptide portion of the compounds targets the compounds to a specific cellular location.
    Type: Grant
    Filed: October 31, 2012
    Date of Patent: January 6, 2015
    Assignee: Marquette University
    Inventor: Daniel S. Sem
  • Publication number: 20140377875
    Abstract: UV-Absorbing and fluorescent pl markers for isoelectric focusing separations and fluorescent labeling, and methods for making and using the markers.
    Type: Application
    Filed: August 27, 2012
    Publication date: December 25, 2014
    Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
    Inventors: Gyula Vigh, Ming-Chien Li
  • Patent number: 8916683
    Abstract: Described herein are block copolymer conjugates that form double-network hydrogels under appropriate conditions. The conjugates comprise a block of polymer end-group, a block of self-associating peptide or protein, and flexible linkers between the two. Hydrogels comprising the conjugates have the mechanical properties, including elastic modulus and fracture toughness, required for load-bearing applications, while maintaining desirable shear-thinning properties, for example, for injectability.
    Type: Grant
    Filed: October 5, 2011
    Date of Patent: December 23, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Bradley D. Olsen, Matthew J. Glassman, Jacqueline Chan
  • Publication number: 20140343188
    Abstract: The embodiments provided herein are directed to methods and systems for generating a customized functionalized substrate. In particular, the embodiments provided herein generate a customized functionalized substrate that can be used for a variety of applications and a variety of chemical and other reactions, processes and methodologies, by modifying a disulfide bond-containing feedstock through the introduction of a disulfide bond breaking material.
    Type: Application
    Filed: August 31, 2012
    Publication date: November 20, 2014
    Applicant: Aspen Research Corporation
    Inventors: John A. Spevacek, Roger Pearson, Kenneth W. Richards, Kenneth D. Zigrino
  • Publication number: 20140323402
    Abstract: The present invention relates to water-soluble protein carrier-linked prodrugs wherein the protein carrier comprises an amino acid sequence consisting of at least 100 amino acid residues forming random coil conformation and comprising alanine, serine and proline residues. It further relates to pharmaceutical compositions comprising said water-soluble protein carrier-linked prodrugs, their use as a medicament as well as methods of treatment and administration.
    Type: Application
    Filed: August 10, 2012
    Publication date: October 30, 2014
    Applicant: Ascendis Phama A/S
    Inventors: Ulrich Hersel, Dirk Vetter
  • Patent number: 8865188
    Abstract: Provided herein are methods and compositions for controlling assembly of modified viral core proteins, for example, into a viral capsid or a nanocage. In some embodiments, the disclosed modified viral core proteins comprise at least one mutation or modification that can substantially prevent assembly of the viral core proteins until assembly is desired. In some embodiments, assembly of the viral core proteins may be triggered, for example, by contacting the viral core proteins with a reducing agent and/or by reducing the concentration of a denaturant. The viral core proteins may self-assemble to form a viral capsid or nanocage.
    Type: Grant
    Filed: September 10, 2012
    Date of Patent: October 21, 2014
    Assignee: Biomed Realty, L.P.
    Inventors: Miguel de los Rios, Stephanie de los Rios, Jacek Ostrowski, Kenneth J. Oh, Ilan Zipkin
  • Publication number: 20140295437
    Abstract: The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.
    Type: Application
    Filed: December 17, 2013
    Publication date: October 2, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Rosaria HAUGLAND, Ching-Ying CHEUNG, Stephen YUE
  • Publication number: 20140273153
    Abstract: The present disclosure provides a method of covalently modifying a biological macromolecule, the method comprising subjecting a reaction mixture comprising: (a) a biological macromolecule comprising one or more thiol groups; and (b) a molecule comprising one or more olefin or alkyne moieties to a radical reaction under conditions sufficient to produce the covalently modified biological macromolecule. The present disclosure also provides a method of covalently modifying a biological macromolecule, the method comprising subjecting a reaction mixture comprising: (a) a molecule comprising one or more thiol groups; and (b) a biological macromolecule comprising one or more olefin or alkyne moieties to a radical reaction under conditions sufficient to produce the covalently modified biological macromolecule. The present disclosure further provides a covalently modified biological macromolecule prepared by any of the disclosed methods.
    Type: Application
    Filed: March 13, 2014
    Publication date: September 18, 2014
    Applicant: Mosaic Biosciences, Inc.
    Inventors: Alexei KAZANTSEV, Peter D. MARINER, Martin STANTON
  • Publication number: 20140121352
    Abstract: Reversible pegylated drugs are provided by derivatization of free functional groups of the drug selected from amino, hydroxyl, mercapto, phosphate and/or carboxyl with groups sensitive to mild basic conditions such as 9-fluorenylmethoxycarbonyl (Fmoc) or 2-sulfo-9-fluorenylmethoxycarbonyl (FMS), to which group a PEG moiety is attached. In these pegylated drugs, the PEG moiety and the drug residue are not linked directly to each other, but rather both residues are linked to different positions of the scaffold Fmoc or FMS structure that is highly sensitive to bases and is removable under physiological conditions. The drugs are preferably drugs containing an amino group, most preferably peptides and proteins of low or medium molecular weight. Similar molecules are provided wherein a protein carrier or another polymer carrier replaces the PEG moiety.
    Type: Application
    Filed: January 2, 2014
    Publication date: May 1, 2014
    Applicant: YEDA RESEARCH AND DEVELOPMENT CO., LTD.
    Inventors: YORAM SHECHTER, MATITYAHU FRIDKIN, HAIM TSUBERY
  • Patent number: 8691151
    Abstract: This invention provides an apparatus for particle sorting, particle patterning, and methods of using the same. The sorting or patterning is opto-fluidics based, in that particles are applied to individual chambers in the device, detection and/or analysis of the particles is carried out, such that a cell or population whose removal or conveyance is desired is defined, and the cell or population is removed or conveyed via application of an optical force and flow-mediated conveyance or removal of the part.
    Type: Grant
    Filed: August 27, 2007
    Date of Patent: April 8, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Joseph Kovac, Joel Voldman
  • Publication number: 20140088021
    Abstract: Compounds comprising a binding moiety, water-soluble, non-peptidic polymer, and drug are provided. Also provided are methods preparing such compounds, compositions comprising such compounds and methods for administering and using such compounds and compositions.
    Type: Application
    Filed: May 24, 2012
    Publication date: March 27, 2014
    Applicant: Nektar Therapeutics
    Inventors: Jennifer Riggs-Sauthier, Deborah H. Charych, Clark Norman Eid, Dennis G. Fry, Marina Konakova, Christine Frances Loehrlein
  • Patent number: 8673215
    Abstract: The invention relates to the test device for platelet aggregation detection comprising: —an element (1) for receiving a blood sample—a capillary tube (3) connected at a first end (31) to said element (1) and at a second end (32) to a pressure lowering device (5) to pump said blood sample through said capillary tube (3)—at least a pair of facing electrodes (8) on the capillary tube—a device for measuring an impedance between said pair of facing electrodes. The invention also relates to a process for using this device, comprising: a) receiving a blood sample and pumping it through the capillar tube (3) b) determining a dynamic change of the value of the impedance between at least one pair of electrodes (8).
    Type: Grant
    Filed: October 16, 2008
    Date of Patent: March 18, 2014
    Assignee: Centre National de la Recherche Scientifique
    Inventors: Francine Rendu, Daniel Fruman, Jaime Levenson
  • Publication number: 20140072992
    Abstract: Methods for detection of the activity of proteolytic enzymes, particularly isopeptidases, are disclosed.
    Type: Application
    Filed: August 26, 2013
    Publication date: March 13, 2014
    Applicants: Progenra, Inc., Lifesensors, Inc.
    Inventors: Dattananda Chelur, Michael Eddins, Craig Leach, Steven J. Orcutt, Raymond J. Peroutka, James Strickler, Yilin Yan, Kathryn Longenecker
  • Publication number: 20140058070
    Abstract: There are provided compounds and methods for amidating the C-terminus of a polypeptide. The method include reacting a polypeptide which includes a C-terminal thioester or C-terminal selenoester with any one of a defined set of auxiliary molecules under conditions suitable to produce a polypeptide adduct which includes the auxiliary molecule chemically bound at the C-terminal of the polypeptide. In the subsequent step, the auxiliary molecule is removed from the C-terminal of the polypeptide adduct under conditions suitable to produce an amidated polypeptide.
    Type: Application
    Filed: September 8, 2011
    Publication date: February 27, 2014
    Applicant: AstraZeneca Pharmaceuticala LP
    Inventors: Chengzao Sun, Behrouz Bruce Forood, Gary Luo, Soumitra S. Ghosh
  • Patent number: 8652474
    Abstract: A composition comprising a main species HER2 antibody that binds to domain II of HER2 and acidic variants thereof is described. Pharmaceutical formulations comprising the composition, and therapeutic uses for the composition are also disclosed.
    Type: Grant
    Filed: January 28, 2009
    Date of Patent: February 18, 2014
    Assignee: Genentech, Inc.
    Inventors: Reed J. Harris, Paul A. Motchnik
  • Patent number: 8652409
    Abstract: Fluid analyte sensors include a photoelectrocatalytic element that is configured to be exposed to the fluid, if present, and to respond to photoelectrocatalysis of at least one analyte in the fluid that occurs in response to impingement of optical radiation upon the photoelectrocatalytic element. A semiconductor light emitting source is also provided that is configured to impinge the optical radiation upon the photoelectrocatalytic element. Related solid state devices and sensing methods are also described.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: February 18, 2014
    Assignee: Valencell, Inc.
    Inventors: Steven Francis LeBoeuf, Jesse Berkley Tucker, Michael Edward Aumer
  • Publication number: 20140046040
    Abstract: Methods and compositions for the selective derivatization of a oligohistidine-tagged recombinant protein. A modifying compound comprised of an imidazole reactive group, a linker, and a ligating group is contacted with the recombinant protein, and a cooperative bond forms between the ligating group and the oligohistidine tag in the presence of a metal cation, and a covalent bond forms between the imidazole reactive group and an imidazole ring of the oligohistidine tag followed by the concomitant separation of the imidazole reactive group from the linker. Addition of a metal chelator results in the dissociation of the ligating group and the oligohistidine tag.
    Type: Application
    Filed: August 8, 2013
    Publication date: February 13, 2014
    Applicant: CLARKSON UNIVERSITY
    Inventor: Artem Melman
  • Patent number: 8647575
    Abstract: A blood glucose meter includes a front attachment part to which a test piece is attached, a measurement part for measuring a component of blood collected via a blood guide passage in the test piece, and a monitor for displaying the measurement results obtained by the measurement part. When the device is placed on a horizontal plane by referring to the display face of the monitor as the upper side and the opposite side as the lower side and placing the display face of the monitor upward, the central axis of the test piece extends obliquely downward toward the front side. The blood glucose meter comprises a main part provided with the monitor and a linking part between the main part and the front attachment part. The top face of the linking part is placed roughly parallel to the central axis line and is provided with an ejector lever.
    Type: Grant
    Filed: November 19, 2009
    Date of Patent: February 11, 2014
    Assignee: Terumo Kabushiki Kaisha
    Inventor: Hirotaka Ohashi
  • Publication number: 20140039167
    Abstract: The invention conjugates that can be prepared by contacting a polymer comprising an acetal or ketal branching point with a pharmacologically active agent under conditions suitable to form a covalent attachment between the polymer and the pharmacologically active agent.
    Type: Application
    Filed: October 3, 2013
    Publication date: February 6, 2014
    Applicant: Nectar Therapeutics
    Inventors: Samuel P. McManus, Antoni Kozlowski
  • Publication number: 20140031524
    Abstract: The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.
    Type: Application
    Filed: April 17, 2013
    Publication date: January 30, 2014
    Applicant: AnaSpec Incorporated
    Inventor: AnaSpec Incorporated
  • Publication number: 20140024123
    Abstract: Provided is a screening method for a more effective Ras inhibitor. Further provided are amino acid residues at a site important for an interaction with a Ras inhibitor in a Ras polypeptide. By confirming a difference between structural information on the Ras polypeptide by NMR and structural information between a complex of the Ras polypeptide and a seed compound by NMR, a more effective lead compound than the seed compound can be selected. As a result of analysis based on the structural information on the Ras polypeptide by NMR, the amino acid residues capable of interacting with a Ras inhibitor candidate (a) are K5, E37, D38, S39, L56, E63, Y64, A66, M67, Q70, Y71, R73, and T74 with reference to an amino acid sequence set forth in SEQ ID NO: 1.
    Type: Application
    Filed: January 31, 2012
    Publication date: January 23, 2014
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Tohru Kataoka, Fumi Shima, Atsuo Tamuta, Mitsugu Araki
  • Publication number: 20140011208
    Abstract: The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
    Type: Application
    Filed: April 9, 2013
    Publication date: January 9, 2014
    Inventors: Fei Mao, Wai-Yee Leung, Ching-Ying Cheung, Ching-Ying Cheung
  • Publication number: 20140011255
    Abstract: Biotin derivatives, methods of using the biotin derivatives and kits comprising the biotin derivatives.
    Type: Application
    Filed: October 28, 2011
    Publication date: January 9, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Lai-Qiang Ying, Bruce Branchauo, Yu-Zhong Zhang, Stephen Yue
  • Publication number: 20130323168
    Abstract: Provided herein are novel compounds based on xanthene, for example biarsenical compounds, which are useful as molecular probes. The compounds contain at least one isotope atom, such as a radioisotope atom. Methods for visualizing tetracysteine-tagged analyte molecules such as proteins using said compounds, methods for synthesizing said compounds, and methods for treating cancer or other hyperproliferative disorders using said compounds are also provided.
    Type: Application
    Filed: November 15, 2011
    Publication date: December 5, 2013
    Applicant: BIOPERCEPT AB
    Inventors: Hans Eric Peter Strom, Samuel Par Simon Svensson
  • Patent number: 8575282
    Abstract: A modified compound that has at least one further functional group, in particular a bio- or macromolecule, comprising at least one x-fold (x?1) chemoselectively incorporated phosphoramidate group of general formula (I), NPO(OR1)(OR1?), and/or at least one x-fold (x?1) chemoselectively incorporated phosphonamide group of general formula (Ia), NPO(R1)(OR1?). R1 and R1? is selected from the group containing glycerol, polyglycerol, PEG polymers of the general empirical formula C2nH4n+2On+1 with n?1, Cn-alkyl chains with n?1; functionalized Cn-alkyl chains with n?1, aryls, heteroaryl, silyl, lipids, fluorophores, saccharides, peptides, crown ethers, or a linker, which links the aforementioned groups. R1 and R1? can be identical to or different from one another.
    Type: Grant
    Filed: March 9, 2010
    Date of Patent: November 5, 2013
    Assignee: Freie Universitaet Berlin
    Inventors: Christian Hackenberger, Giuseppe Del Signore, Remigiusz Serwa, Ina Wilkening, Robert Vallee
  • Publication number: 20130280814
    Abstract: The present invention relates to a method for labeling proteins in a sample prior to separation thereof using a protein reactive dye, comprising the following steps a) dissolving the proteins in, or diluting the proteins with, or exchanging an existing protein buffer with, a labeling buffer comprising a dye-reactant (reacting with the protein reactive dye) to form a mixture, b) adding protein reactive dye to said mixture, c) incubating said mixture wherein the labeling of said proteins with said dye can be completed within 5 minutes, and wherein both the proteins and the dye-reactant form measurable reaction products with said dye, and d) separating said reaction products. The invention also relates to a kit for pre-labeling of proteins, comprising a labeling buffer, a dye, a molecular weight marker, and a sample gel loading buffer.
    Type: Application
    Filed: May 24, 2011
    Publication date: October 24, 2013
    Applicant: GE HEALTHCARE BIO-SCIENCES AB
    Inventor: Elsemarie Bjellqvist
  • Publication number: 20130281659
    Abstract: The present technology relates to well-defined oligomers comprising two or more monomers wherein each monomer is independently selected from a ubiquitin polypeptide or a ubiquitin-like polypeptide, and the monomers are covalently linked to each other via a thioether group or groups. Further provided are monomer building blocks and methods of making the monomers and oligomers.
    Type: Application
    Filed: March 8, 2013
    Publication date: October 24, 2013
    Inventors: Eric Strieter, Ellen Valkevich, Robert Guenette
  • Publication number: 20130274452
    Abstract: Compounds and methods are disclosed that are useful for noninvasive imaging in the near-infrared spectral range. The cyanine compounds of Formula I are presented: wherein Q is a portion of a polymethine bridge selected from the group consisting of: Also included are bioconjugates of the compounds of Formula I, methods of labeling biomolecules with the compounds, and methods of imaging.
    Type: Application
    Filed: March 14, 2013
    Publication date: October 17, 2013
    Inventors: Xinshe Xu, Daniel R. Draney, Lael Cheung
  • Publication number: 20130266953
    Abstract: Provided herein are fluorescent bioprobes comprising fluorogens that exhibit aggregation-induced emission (AIE) labeled on biomolecules. The present subject matter relates to a fluorescent bioprobe comprising one or more fluorogen labeled on chitosan. The present subject matter is also directed to methods of preparing the fluorescent bioprobes, methods of labeling and detecting DNA and/or proteins with the fluorescent bioprobe, and methods of cell imaging including live cell tracking.
    Type: Application
    Filed: May 21, 2013
    Publication date: October 10, 2013
    Applicant: The Hong Kong University of Science And Technology
    Inventor: The Hong Kong University of Science And Technology
  • Publication number: 20130251637
    Abstract: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
    Type: Application
    Filed: December 20, 2011
    Publication date: September 26, 2013
    Applicants: DYOMICS GMBH, PIERCE BIOTECHNOLOGY, INC.
    Inventors: Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa Dworecki, Frank G. Lehmann
  • Publication number: 20130252897
    Abstract: The present invention concerns the use of polychalcogenide compositions on cells, tissue, organs, and organisms to enhance their survivability. It includes compositions, compounds, methods, articles of manufacture and apparatuses for enhancing survivability and for protecting them from or treating them for injury or damage. In specific embodiments, there are also therapeutic methods and apparatuses for hypoxic/ischemic injury, organ transplantation, hyperthermia, wound healing, hemorrhagic shock, cardioplegia for bypass surgery, neurodegeneration, hypothermia, and cancer using the polychalcogenide compositions described.
    Type: Application
    Filed: September 14, 2012
    Publication date: September 26, 2013
    Applicant: FRED HUTCHINSON CANCER RESEARCH CENTER
    Inventors: Mark B. Roth, Mike Morrison
  • Publication number: 20130252936
    Abstract: Compounds, compositions and methods for the treatment of retinal degenerative diseases, such as retinitis pigmentosa, Leber's congenital Amaurosis, Syndromic retinal degenerations, age-related macular degeneration and Usher Syndrome, and hearing loss associated with Usher Syndrome are described herein.
    Type: Application
    Filed: March 8, 2013
    Publication date: September 26, 2013
    Applicant: Usher III Initiative
    Inventors: Roland Werner BÜRLI, William Rameshchandra Krishna Esmieu, Christopher James Lock, Karine Fabienne Malagu, Andrew Pate Owens, William E. Harte
  • Publication number: 20130230465
    Abstract: Compounds useful as labels with properties comparable to known fluorescent compounds. The compounds are conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
    Type: Application
    Filed: February 27, 2013
    Publication date: September 5, 2013
    Inventors: Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa R. Dworecki, Frank G. Lehmann, Marie Christine Nlend
  • Publication number: 20130211055
    Abstract: Dithioamine reducing agents useful for the reduction of disulfide bonds. The reducing agents of this invention are useful, for example, to reduce disulfide bonds, particularly in proteins, or to prevent the formation of disulfide bonds, particularly in proteins and other biological molecules. Reducing agents of this invention are useful and suitable for application in a variety of biological applications, particularly as research and synthetic reagents. The invention provides S-acylated dithioamines which can be selectively activated reducing agents by removal of the S-acyl groups enzymatically or chemically. The invention further provides dithiane precursors of thioamino reducing agents. The invention provides dithioamine reducing agents, S-acylated dithioamines and dithianes which are immobilized on surfaces, including among others, glass, quartz, microparticles, nanoparticles and resins.
    Type: Application
    Filed: February 15, 2013
    Publication date: August 15, 2013
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventor: Wisconsin Alumni Research Foundation
  • Patent number: 8507289
    Abstract: The present invention provides a test strip for measuring a concentration of an analyte of interest in a biological fluid, wherein the test strip may be encoded with information that can be read by a test meter into which the test strip is inserted.
    Type: Grant
    Filed: July 16, 2012
    Date of Patent: August 13, 2013
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Henning Groll, Michael J. Celentano
  • Patent number: 8501093
    Abstract: A portable handheld medical diagnostic device includes a housing forming a protective enclosure. A main circuit board is located in the protective enclosure. The main circuit board includes a controller facilitating a physiologic measurement. A display device is connected to the main circuit board that displays information related to the physiologic measurement. An electronic skin is on the housing. The electronic skin comprises a liquid crystal material and is configured to display a color.
    Type: Grant
    Filed: June 11, 2009
    Date of Patent: August 6, 2013
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Paul S. Rutkowski, Robert G. Davies, Mark Mears
  • Publication number: 20130197195
    Abstract: There is provided a method for covalent immobilization of at least one molecule comprising at least one amino group, said method comprising the sequential steps of: a) providing a surface comprising —SH groups, b) oxidizing the surface comprising —SH groups using redox reactions in the presence of noble metal ions, and c) contacting the surface with at least one molecule comprising at least one amino group to obtain a covalent binding of the at least one molecule to the surface, wherein said at least one amino group is involved in obtaining said covalent bond. The immobilized molecules are immobilized via stable covalent bonds. The method is more versatile since it can be performed as a one step method. All reaction steps are performed in aqueous solution. All steps can be performed at room temperature. The chemicals used are less expensive and less toxic compared to the prior art.
    Type: Application
    Filed: July 27, 2011
    Publication date: August 1, 2013
    Applicant: LAB-ON-A-BEAD AB
    Inventors: Sven Oscarsson, Kristofer Eriksson, Leif Nyholm
  • Publication number: 20130184444
    Abstract: A water soluble nanoparticle can include a ligand of formula (I). The ligand can provide zwitterionic character and can provide water solubility, small hydrodynamic diameter, chemical stability, and the capability to modify the nanoparticle with additional functional moieties such as a small molecule, nucleic acid, or protein.
    Type: Application
    Filed: December 15, 2012
    Publication date: July 18, 2013
    Applicant: Massachusetts Institute of Technology
    Inventor: Massachusetts Institute of Technology
  • Publication number: 20130183327
    Abstract: The present disclosure provides IDAC compounds capable of presenting two or more signal 1 moieties to a host immune system and methods of using the IDAC compounds to treat or prevent autoimmune disorders in a subject. The present disclosure provides compounds including a modified I-domain peptide having two or more modified lysine residues and two or more signal 1 moieities conjugated to the modified lysine residues of the I-domain peptide and methods of using an making the compounds.
    Type: Application
    Filed: January 4, 2013
    Publication date: July 18, 2013
    Applicant: UNIVERSITY OF KANSAS
    Inventor: UNIVERSITY OF KANSAS