Ultra Filtration Or Osmosis Patents (Class 530/414)
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Patent number: 8470969Abstract: The present invention relates generally to Apo2L/TRAIL purification involving crystallization.Type: GrantFiled: June 23, 2010Date of Patent: June 25, 2013Assignee: Genentech, Inc.Inventors: Heather Flores, Tanya P. Lin, Timothy C. Matthews, Roger Pai, Zahra Shahrokh, Evan E. Shave, Patricia A. Rancatore
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Patent number: 8378073Abstract: The present invention provides a process for attaching one or more effector molecules to one or more cysteines in a protein comprising: a) activating one or more cysteines in the protein by diafiltering the protein against a monothiol reducing agent or a multi-thiol reducing agent which is incapable of forming intramolecular disulphide bonds and b) reacting the treated protein with an effector molecule.Type: GrantFiled: June 29, 2006Date of Patent: February 19, 2013Assignee: UCB Pharma S.A.Inventor: Sam Philip Heywood
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Patent number: 8367810Abstract: Disclosed are substantially purified Plasmodium sporozoites and preparations of Plasmodium sporozoites substantially separated from attendant non-sporozoite material, where the preparations of Plasmodium sporozoites have increasing levels of purity. Vaccines and pharmaceutical compositions comprising purified Plasmodium sporozoites are likewise provided. Methods of purifying preparations of Plasmodium sporozoites are also provided.Type: GrantFiled: August 27, 2010Date of Patent: February 5, 2013Assignee: Sanaria Inc.Inventors: B. Kim Lee Sim, Minglin Li, Richard E. Stafford, Stephen L. Hoffman
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Patent number: 8354505Abstract: A concentrate of Von Willebrand Factor (VWF) or a complex of Factor VIII/VWF is prepared by creating a solution of VWF or a complex of Factor VIII/VWF containing VWF at a concentration of up to 12 IU VWF:RCo/ml and a VWF/Factor VIII ratio of 0.4 or more; and nanofiltering that starting solution through a filter of pore size of 35 nanometers or smaller. The resulting VWF retains high molecular weight multimers.Type: GrantFiled: January 7, 2009Date of Patent: January 15, 2013Assignee: Grifols, S.A.Inventors: Pere Ristol Debart, Maria Mercedes Faro Tomas, Juan Ignacio Jorquera Nieto
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Patent number: 8344108Abstract: Methods and apparatus for processing corn into one or more corn products. Zein is extracted from corn or corn products or by-products with a solvent. The corn-solvent mixture is separated into streams, one of which preferably includes an extract containing at least zein and solvent, and another that contains de-zeined corn solids and adsorbed solvent. The solvent is separated from the zein, and the de-zeined, desolventized corn solids are processed to provide one or more corn products.Type: GrantFiled: July 21, 2009Date of Patent: January 1, 2013Assignee: The Board of Trustees of the University of IllinoisInventor: Munir Cheryan
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Patent number: 8344107Abstract: Methods and apparatus for processing corn into one or more corn products. Oil is extracted from corn or corn products or by-products with a solvent. The corn-solvent mixture is separated into streams, one of which preferably includes an extract containing at least oil and solvent, and another containing de-oiled corn solids and adsorbed solvent. Zein is separated from the de-oiled corn solids. Solvent is then separated, and the de-oiled, de-zeined, desolventized corn solids are processed to provide one or more corn products.Type: GrantFiled: July 14, 2009Date of Patent: January 1, 2013Assignee: The Board of Trustees of the University of IllinoisInventor: Munir Cheryan
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Publication number: 20120283422Abstract: An improved ethanol production process providing novel stillage treatment is disclosed wherein the stillage is separated into four value added product streams that are subjected to drying conditions reducing or eliminating volatization of any VOC's in the product streams.Type: ApplicationFiled: July 17, 2012Publication date: November 8, 2012Inventors: Neal Hammond, John Prevost
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Publication number: 20120269948Abstract: A novel canola protein product consisting predominantly of 2S canola protein and having improved solubility properties, has an increased proportion of 2 S canola protein and a decreased proportion of 7S canola protein, and a protein content of less than about 90 wt % (N×6.25) d.b. The novel canola protein isolate is formed by heat treatment or isoelectric precipitation of aqueous supernatant from canola protein micelle formation and precipitation, to effect precipitation of 7S protein which is sedimented and removed.Type: ApplicationFiled: September 14, 2010Publication date: October 25, 2012Inventors: Sarah Medina, Martin Schweizer, Brent E. Green, Kevin I. Segall, Randy Willardsen, James Logie
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Publication number: 20120264920Abstract: The invention is directed to a method for purifying a protein. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, inactivating viruses in the sample, and processing through at least one depth filter and ion-exchange membrane.Type: ApplicationFiled: October 11, 2011Publication date: October 18, 2012Applicant: ABBOTT LABORATORIESInventors: Chen Wang, Robert K. Hickman
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Publication number: 20120263852Abstract: The invention pertains to process for manufacturing whey protein concentrate (WPC) from whey, said process involving (a) providing acidified whey; (b) increasing the pH of said acidified whey using one or more carbonate salt(s), preceded and/or followed by ultrafiltration, and (c) subjecting the ultrafiltered carbonate-containing whey to spray drying. A WPC is provided having improved functional properties, particularly increased gel strength and reduced salt sensitivity (i.e. meaning that the functional properties of the WPC are affected by salt to a lesser extent).Type: ApplicationFiled: October 11, 2010Publication date: October 18, 2012Inventors: Suzanne Godelieve Thiessen-Bolder, Linqiu Cao, Iliana Hidalgo Torres
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Patent number: 8129507Abstract: An atopic dermatitis inducer binding to a human own IgE antibody and activating mast cells and basophiles, which includes a purified human secretion fraction, or an antigenic molecule or an antigenic determinant in the purified fraction, and obtained through the following steps of: filtering a human secretion, removing insoluble matters and collecting the filtrate; mixing the filtrate with a ConA-affinity carrier and collecting the supernatant; and separating a component having a histamine-releasing activity from the supernatant by column chromatography. This inducer is effective in diagnosing and treating human atopic dermatitis.Type: GrantFiled: August 10, 2009Date of Patent: March 6, 2012Assignee: Shionogi & Co., Ltd.Inventors: Michihiro Hide, Toshihiko Tanaka, Akio Tanaka, Kaori Ishii, Hidenori Suzuki
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Publication number: 20120029165Abstract: The present invention concerns methods of isolating milk proteins. Methods of the invention include charged ultrafiltration processes that use variations in pH to further separate protein species.Type: ApplicationFiled: July 12, 2011Publication date: February 2, 2012Inventors: Mark R. Etzel, Thatcher Root, Abhiram Arunkumar, Seyhun Gemili
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Publication number: 20120028878Abstract: The present invention relates to methods of generating highly concentrated protein solutions, e.g., an antibody solution, a therapeutic protein solution, etc. The methods of the invention include membrane evaporation, such as evaporation of protein-free solvent from the protein solution, which concentrates the protein. The methods of the present invention result in protein solution concentrations not previously achievable by conventional ultrafiltration methods, e.g., protein solution concentrations of greater than about 260 grams of protein per liter of solution.Type: ApplicationFiled: March 24, 2010Publication date: February 2, 2012Applicant: Wyeth LLCInventors: Glen Reed Bolton, Hari Acharya, Austin Wayne Boesch
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Publication number: 20110301342Abstract: The invention is directed to an apparatus and method for purifying a protein. The apparatus involves the use of a capture chromatography resin, a depth filter arranged after the capture chromatography resin, and a mixed-mode chromatography resin arranged after the depth filter. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, a depth filter, and a mixed-mode chromatography resin. A membrane adsorber or monolith may be substituted for the mixed-mode chromatography column.Type: ApplicationFiled: April 13, 2011Publication date: December 8, 2011Applicant: ABBOTT LABORATORIES, INC.Inventors: Chen Wang, Robert K. Hickman, Edwin O. Lundell, Roy D. Hegedus
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Publication number: 20110269947Abstract: The present invention relates generally to Apo2L/TRAIL purification involving crystallization.Type: ApplicationFiled: June 23, 2010Publication date: November 3, 2011Inventors: Heather Flores, Tanya P. Lin, Timothy C. Matthews, Roger Pai, Zahra Shahrokh, Evan E. Shave, Patricia A. Rancatore
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Patent number: 8043866Abstract: A object of the present invention is to provide an immunochromatography method that makes it possible to rapidly detect an ultratrace amount of an analyte that has been impossible to analyze by conventional immunochromatography methods. The present invention provides an immunochromatography method, which comprises developing an analyte and a labeling substance which is modified with a first binding substance against the analyte in a mixed state on a porous carrier and capturing the analyte and the label at a reaction site on the porous carrier having a second binding substance against the analyte or a substance capable of binding to the first binding substance against the analyte, so as to detect the analyte, wherein the labeling substance having an average particle size of 1 ?m or more and 20 ?m or less is detected.Type: GrantFiled: September 29, 2008Date of Patent: October 25, 2011Assignee: Fujifilm CorporationInventors: Takayoshi Oyamada, Junichi Katada
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Publication number: 20110213126Abstract: The present invention provides a method for concentrating a protein, in particular a method for concentrating a plasma product, in particular IgG, using glycine in a (two-stage ultrafiltration/diafiltration approach.Type: ApplicationFiled: April 14, 2009Publication date: September 1, 2011Applicant: Talecris Biotherapeutics, Inc.Inventors: Martin Gonzalez, Woody D. Wood, Fred H. Earp
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Patent number: 7993877Abstract: A process is provided for the preparation of a highly pure recombinant albumin solution having a nickel ion content of less than 100 ng per gram of albumin. The process comprises subjecting a recombinant albumin to a series of chromatography, concentration, and diafiltration steps.Type: GrantFiled: September 9, 2008Date of Patent: August 9, 2011Assignee: Novozymes Biopharma DK A/SInventors: Hendrik Van Urk, David John Mead, Philip Harvey Morton, Andrew John Cartwright, Jason Cameron, David James Ballance, Michel Gaston Joseph Grandgeorge, Stephen Berezenko, John Rodney Woodrow, Darrell Sleep, Jean-Luc Bernard Veron
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Patent number: 7932355Abstract: La present invention pertains to a composition of plasma-derived virally-safe factor VIII, obtained after nanometric filtration, said composition comprising von Willebrand factor (vWF) at a rate of 15% or less of decamers and higher multimers. Such compositions have a reduction factor of the virus titre higher than 4 log and are thus suitable for treatment of hemophilia.Type: GrantFiled: October 25, 2004Date of Patent: April 26, 2011Assignee: LFB SAInventors: Abdessatar Chtourou, Michel Nogre, Roland Schmitthaeusler
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Patent number: 7906024Abstract: The addition of buffer to a harvest stream during diafiltration can cause increased turbidity and have other undesirable effects including limiting product recovery. Methods and compositions related to the use of a non-ionic surfactant are provided for improving diafiltration.Type: GrantFiled: January 24, 2006Date of Patent: March 15, 2011Assignee: Wyeth Research Ireland LimitedInventors: James Pierce, Enda Moran
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Publication number: 20110054153Abstract: The present invention relates generally to Apo2L/TRAIL purification involving crystallization.Type: ApplicationFiled: May 10, 2006Publication date: March 3, 2011Inventors: Heather Flores, Tanya Lin, Timothy Matthews, Roger Pai, Zahra Shahrokh
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Publication number: 20110015377Abstract: The method of the present invention comprising successive column chromatography processes for the purification of an anthrax protective antigen can achieve an improved purity of the anthrax protective antigen product by effectively removing impurities (e.g., cellular residual proteins in the culture solution) without the loss of anthrax protective antigen. Therefore, the method of the present invention can be advantageously used for economically producing the anthrax protective antigen on a large scale.Type: ApplicationFiled: January 9, 2009Publication date: January 20, 2011Inventors: Hee-Bok Oh, Bong-Su Kim, Gi-Eun Rhie, Jeong-Hoon Chun, Hun Kim, Sinkoo Yeo, Mahnhoon Park, Chong-Hwan Jonathan Chang, Mi Sun Ahn
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Patent number: 7872108Abstract: Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological materials are provided. In one aspect of the invention, the compositions, hereinafter termed “proteons” are comprised of misfolded proteins. Also provided are compositions and methods for the isolation and manipulation of proteon nucleation centers (PNCs) upon which the proteons of the present in blood and other biological materials form. In another aspect of the invention, the PNCs are comprised of metallic nanoclusters.Type: GrantFiled: April 22, 2008Date of Patent: January 18, 2011Assignee: Auburn UniversityInventors: Vitaly J. Vodyanoy, Alexandre M. Samoylov, Oleg M. Pustovyy
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Publication number: 20100331530Abstract: Method for purifying therapeutic proteins by multi-stage extractive distillation.Type: ApplicationFiled: February 25, 2009Publication date: December 30, 2010Applicant: BAYER TECHNOLOGY SERVICES GMBHInventors: Werner Bäcker, Sven Sommerfeld, Martina Mutter, Paula Alexandra Albuquerque De Jesus Rosa, Maria Raquel Murias Dos Santos Aires-Barosl, Ana Margarida Nunes Da Mata Pires De Azevedo
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Publication number: 20100303958Abstract: Embodiments of the invention are directed to a process involving microfiltration of milk, providing a product that is useful as a source of casein and another product that is useful as a source of whey protein. Another embodiment of the invention is directed to preparing milk products like casein, dairy beverages, milk protein concentrates and cheese from microfiltration retentate.Type: ApplicationFiled: November 2, 2008Publication date: December 2, 2010Inventors: Shakeel Ur-Rehman, John M. Dunker
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Publication number: 20100291189Abstract: A method is provided for producing a membrane protein folded to its native structure or active structure in a lipid disc or a liposome. The method comprises: (a) preparing a reaction solution for cell-free protein synthesis containing a polynucleotide encoding a membrane protein, a steroidal detergent, and a phospholipid, wherein the steroidal detergent is contained at a concentration higher than its critical micelle concentration; (b) decreasing the concentration of said steroidal detergent in the reaction solution; and (c) synthesizing the membrane protein simultaneously with formation of a lipid disk or liposome into which the synthesized membrane protein is integrated.Type: ApplicationFiled: November 5, 2008Publication date: November 18, 2010Inventors: Shigeyuki Yokoyama, Kazumi Shimono, Mikako Shirouzu, Mie Goto
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Publication number: 20100291285Abstract: The supernatant from the deposition of canola protein micellar mass is processed to provide a canola protein product having a protein content of about 60 to less than about 90 wt % (N×6.25) protein on a dry weight basis and which is soluble in an aqueous acidic environment.Type: ApplicationFiled: April 26, 2010Publication date: November 18, 2010Inventors: Sarah Medina, Kevin I. Segall, Brent E. Green, Martin Schweizer
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Patent number: 7754860Abstract: The invention provides a method for purifying recombinant FSH.Type: GrantFiled: December 16, 2004Date of Patent: July 13, 2010Assignee: Ares Trading S.A.Inventor: Mara Rossi
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Publication number: 20100172894Abstract: Processes and methods of purifying or separating Single Domain Antigen Binding (SDAB) molecules that include one or more single binding domains (e.g., one or more nanobody molecules), substantially devoid of a complementary antibody domain and an immunoglobulin constant region, using Protein A-based affinity chromatography, are disclosed.Type: ApplicationFiled: October 29, 2009Publication date: July 8, 2010Applicant: WYETHInventors: Paul R. Brown, Scott Andreas Tobler, Andrew M. Wood, Austin Wayne Boesch
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Patent number: 7744890Abstract: Methods for reducing the opalescent appearance of a protein preparation by modifying the ionic strength of the preparation, as well as compositions, e.g., pharmaceutical compositions, of concentrated protein with decreased opalescence are disclosed. Purification methods which monitor and/or reduce the salt concentrations at selected steps are also disclosed.Type: GrantFiled: October 12, 2007Date of Patent: June 29, 2010Assignee: Wyeth LLCInventors: Pilarin Elizabeth Nichols, Donna L. Luisi
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Patent number: 7728116Abstract: In a method of isolating osteogenic protein from bone, in which an osteogenic protein-containing fraction is extracted from bone and enriched by a sequence of enrichment steps selected from ultrafiltration and chromatography, the invention provides the improvement of removing higher molecular weight components from the osteogenic protein-containing fraction prior to the enrichment steps. The higher molecular weight components have a molecular weight of about 100-300 kDa and are selected from collagen, collagen fragments, collagen aggregates and mixtures thereof.Type: GrantFiled: June 18, 2003Date of Patent: June 1, 2010Assignee: Altis Biologics (Proprietary) LimitedInventor: Nicolaas Duneas
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Publication number: 20100111853Abstract: Anti-IL-12 antibodies are disclosed herein, including antigen-binding portions thereof. One or more methods for isolating and purifying anti-IL-12 antibodies from a sample matrix is presented. These isolated anti-IL-12 antibodies can be used in a clinical setting as well as in research and development. Pharmaceutical compositions comprising isolated anti-IL-12 antibodies are also described.Type: ApplicationFiled: October 20, 2009Publication date: May 6, 2010Applicant: Abbott Laboratories, Inc.Inventors: Robert K. Hickman, Qing Huang, Barbara Perilli-Palmer
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Patent number: 7674885Abstract: The invention provides methods for concentrating a macromolecule from a solution comprising the macromolecule and an organic polymer by first subjecting the solution to ultrafiltration to produce a first retentate solution, then adjusting the conductivity of the first retentate solution such that any protein precipitation induced by the organic polymer is essentially prevented to produce a second retentate solution, and then subjecting the second retentate solution to ultrafiltration. In a preferred embodiment, the conductivity is adjusted by diafiltration against water, suitable diluent or buffer. Preferably, the invention pertains to the concentration of solutions of native or recombinant proteins. The invention further pertains preferably to methods for the concentration of cell culture supernatant comprising a product protein and organic polymers of the PLURONIC family of nonionic block co-polymers, and more preferably comprising PLURONIC F-68 nonionic block co-polymer.Type: GrantFiled: November 1, 2003Date of Patent: March 9, 2010Assignee: Bayer HealthCare LLCInventors: Konstantin Konstantinov, Huong Nguyen, Jens H. Vogel
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Patent number: 7667009Abstract: A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided.Type: GrantFiled: April 14, 2004Date of Patent: February 23, 2010Assignee: Juridical Foundation the Chemo-Sero-Therapeutic Research InstituteInventors: Satoshi Koyanagi, Kenjiro Kawatsu, Toshio Murakami, Yoshinobu Miyatsu, Toshihiro Maeda, Hiroshi Mizokami
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Publication number: 20100040763Abstract: The supernatant from the deposition of canola protein micellar mass is processed to provide a canola protein isolate which is soluble in an aqueous acidic environment.Type: ApplicationFiled: August 18, 2009Publication date: February 18, 2010Inventors: Kevin I. Segall, Brent E. Green, Martin Schweizer
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Patent number: 7662930Abstract: Various embodiments of the present invention are directed to multi-step systems and methods for target-molecule purification that employ column-chromatography-based and/or membrane-filtration-based polishing steps. In one described embodiment of the present invention, a target-protein-containing eluate having a high residual salt concentration is collected from a first chromatography column prepared with an affinity-chromatography resin, loaded onto a second chromatography column prepared with a cation-exchange resin, and eluted from the second cation-exchange column using a buffer in which a time-dependent pH gradient is established. In another described embodiment of the present invention, a partially purified target-protein-containing eluate is collected from a chromatography column and further purified by passing the target-protein-containing eluate through a salt-tolerant anion exchanger.Type: GrantFiled: December 6, 2006Date of Patent: February 16, 2010Assignee: AMGEN Inc.Inventor: Joe Xin Hua Zhou
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Publication number: 20100036099Abstract: Canola protein isolates are provided which contain both albumin and globulin protein fractions that are soluble, transparent and heat stable in an acidic aqueous environment. The canola protein isolates are completely soluble in water at low pH, low in phytic acid and useful in products for human consumption, pet foods and aquaculture.Type: ApplicationFiled: October 13, 2009Publication date: February 11, 2010Inventors: Martin Schweizer, Brent E. Green, Kevin I. Segall, James Logie
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Patent number: 7601515Abstract: A process is provided for the preparation of albumin from a yeast culture medium which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises separating the yeast from the culture medium to yield an albumin solution; subjecting the albumin solution to positive mode cation exchange, affinity, and positive mode anion exchange chromatography; and concentrating and formulating the albumin with sodium octanoate and sodium chloride.Type: GrantFiled: April 23, 2007Date of Patent: October 13, 2009Assignee: Novozymes Biopharma UK LimitedInventors: Andrew Robert Goodey, Darell Sleep, Hendrik Van Urk, Stephen Berezenko, John Rodney Woodrow, Richard Alan Johnson, Patricia Carol Wood, Stephen James Burton, Alan Victor Quirk
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Publication number: 20090214522Abstract: The present disclosure provides a process for concentrating proteins including an ultrafiltering, a diafiltering, and a second ultrafiltering sequence, at elevated temperatures, such as above about 30° C. The disclosure also includes a process for preparing highly concentrated antibody compositions, and highly concentrated antibody products.Type: ApplicationFiled: February 19, 2009Publication date: August 27, 2009Applicants: Genentech, Inc., Novartis AGInventor: Charles M. WINTER
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Patent number: 7569671Abstract: Methods and apparatus for processing corn into one or more corn products. Oil is extracted from corn or corn products or by-products with a solvent. The corn-solvent mixture is separated into streams, one of which preferably includes an extract containing at least oil and solvent, and another containing de-oiled corn solids and adsorbed solvent. The solvent is separated from oil, and the de-oiled, desolventized corn solids are processed to provide one or more corn products.Type: GrantFiled: January 6, 2006Date of Patent: August 4, 2009Assignee: The Board of Trustees of the University of IllinoisInventor: Munir Cheryan
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Publication number: 20090186395Abstract: A method for purifying a protein solution from infective particles comprising (a) adding a macromolecule to the solution; and (b) passing the solution through a nanofilter. The macromolecule may be selected from a polymer of at least 3 monomers of sugars, amino acids, glycols, alcohols, lipids or phospholipids.Type: ApplicationFiled: June 25, 2007Publication date: July 23, 2009Inventors: Israel Nur, Roni Mintz
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Publication number: 20090169568Abstract: The present invention provides a purified multimeric complex form P. gingivalis. The complex comprises at least one domain from each of RgpA, Kgp and HagA, and has a molecular weight greater than about 300 kDa.Type: ApplicationFiled: September 23, 2005Publication date: July 2, 2009Applicant: THE UNIVERSITY OF MELBOURNEInventors: Eric Charles Reynolds, Neil Martin O'Brien-Simpson, Rishi Delan Pathirana
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Patent number: 7553938Abstract: A method of preparing a purified, virus inactivated and virus safe antibody preparation from a starting solution comprising antibodies and contaminants, the method comprising the steps of: (a) adjusting the pH of the starting solution to about 4.6 to about 4.95 in particular to about 4.8 to about 4.95 to produce an intermediate solution; (b) adding caprylate and/or heptanoate ions to the intermediate solution and maintaining the pH at about 4.6 to about 4.95 in particular pH at about 4.8 to about 4.Type: GrantFiled: February 25, 2005Date of Patent: June 30, 2009Assignee: Octapharma AGInventors: Andrea Buchacher, Günther Iberer, Jürgen Römisch
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Patent number: 7524932Abstract: A method for producing an oligomeric form of ?-lactalbumin which is in the molten globule-like state is described. The method suitably comprises exposing a source of ?-lactalbumin in which the ?-lactalbumin is preferably in the molten globule-like state, to an ion exchange medium which has been pre-treated with casein or an active component thereof, such as oleic acid, and recovering ?-lactalbumin in an oligomeric form therefrom. Pre-treatment of the ion exchange medium, particularly with casein derived from human milk, has been found to significantly improve yields of the oligomeric form of ?-lactalbumin and mean that it can readily isolated from readily available sources such as bovine ?-lactalbumin. This form of ?-lactalbumin is useful therapeutically, in particular as an antibacterial agent and also as an anti-cancer therapeutic.Type: GrantFiled: January 27, 2006Date of Patent: April 28, 2009Assignee: Nya Hamlet Pharma ABInventors: Catharina Svanborg, Per Anders Hakansson, Malin Wilhelmina Svensson
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Patent number: 7521417Abstract: A process is disclosed for the preparation of an essentially tetramer-free, substantially stroma-free, polymerized, pyridoxylated hemoglobin. Also disclosed is an essentially tetramer-free, substantially stroma-free, polymerized, pyridoxylated hemoglobin product capable of being infused into human patients in an amount of up to about 5 liters.Type: GrantFiled: January 10, 2008Date of Patent: April 21, 2009Assignee: Northfield Laboratories, Inc.Inventors: Richard E. DeWoskin, Marc D. Doubleday
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Patent number: 7476726Abstract: The present invention provides a method for recombinant production, recovery and purification of endostatin protein. This method may be employed for large scale recovery and purification of recombinantly-produced endostatin protein.Type: GrantFiled: September 14, 2000Date of Patent: January 13, 2009Assignees: The Children's Medical Center Corp., Covance Biotechnology Services, Inc.Inventors: Hong Liang, Kim Lee Sim, Amy Chang-Murad, Xinhua Zhou, John Madsen, Renee J. Boerner, Firoz Rustom Mistry, Scot R. Shepard, Jeffrey L. Schrimsher, Lourdes L. Bermejo
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Publication number: 20080269468Abstract: The present invention relates to a process and apparatus for purifying a molecule of interest from a heterogeneous clarified fluid mixture. The apparatus of the invention generally comprises a continuous perfusion fermentation system, a continuous particle removal system integrated with the perfusion fermentation system; and a continuous purification system integrated with the particle removal system, which is maintained under sterile conditions. The process comprises filtering a heterogeneous clarified fluid mixture by continuous ultrafiltration at a specific flow rate below the transition point of the molecule of interest in the pressure-dependent region of the flux versus TMP curve, wherein the specific flow rate is maintained substantially constant throughout the continuous ultrafiltration.Type: ApplicationFiled: September 30, 2005Publication date: October 30, 2008Applicant: BAYER HEALTHCARE LLCInventors: Jens Holger Vogel, Roberto Giovannini, Konstantin B. Konstantinov, Huong My Nguyen, Paul Wu
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Patent number: 7442308Abstract: A process for removing viruses in fibrinogen solutions and fibrinogen obtained thereof wherein the process starts with an adjusted purified fibrinogen solution, the adjusted purified solution is frozen and then thawed at a temperature between 5 and 20° C., the undissolved materials associated with the fibrinogen are subsequently separated, the temperature is adjusted and the resultant solution is finally subjected to nanofiltration using filters having a pore size smaller than 35 nm.Type: GrantFiled: February 25, 2004Date of Patent: October 28, 2008Assignee: Grifols, S.A.Inventors: Pere Ristol Debart, Jesus Fernandez Rodriguez
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Publication number: 20080262206Abstract: Compositions and methods for the isolation and manipulation of misfolded, or partially misfolded, proteins present in blood and other biological materials are provided. In one aspect of the invention, the compositions, hereinafter termed “proteons” are comprised of misfolded proteins. Also provided are compositions and methods for the isolation and manipulation of proteon nucleation centers (PNCs) upon which the proteons of the present in blood and other biological materials form. In another aspect of the invention, the PNCs are comprised of metallic nanoclusters.Type: ApplicationFiled: April 22, 2008Publication date: October 23, 2008Applicant: Auburn UniversityInventors: Vitaly J. Vodyanoy, Alexandre M. Samoylov, Oleg M. Pustovyy
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Patent number: RE42315Abstract: The present invention provides a nanostructured device comprising a substrate including nanotroughs therein; and a lipid bilayer suspended on or supported in the substrate. A separation method is also provided comprising the steps of supporting or suspending a lipid bilayer on a substrate; wherein the substrate comprises nanostructures and wherein the lipid bilayer comprises at least one membrane associated biomolecule; and applying a driving force to the lipid bilayer to separate the membrane associated biomolecule from the lipid bilayer and to drive the membrane associated biomolecule into the nanostructures.Type: GrantFiled: July 5, 2007Date of Patent: May 3, 2011Assignee: STC.UNMInventors: Gabriel P. Lopez, Steven R. J. Brueck, Linnea K. Ista