Selective Absorbtion, E.g., Ca Phosphate Sorbents, Etc. Patents (Class 530/415)
  • Patent number: 9789481
    Abstract: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
    Type: Grant
    Filed: October 24, 2007
    Date of Patent: October 17, 2017
    Assignee: Cepheid
    Inventors: Kurt E. Petersen, Michael T. Taylor, Farzad Pourahmadi, William A. McMillan, Ronald Chang, Stanley H. Sakai, Jesus Ching, Douglas B. Dority, Phillip Belgrader, M. Allen Northrup
  • Patent number: 9487566
    Abstract: The present invention relates to protein purification. More particularly, a method for directly recovering an objective protein from a protein composition and purifying a protein with a desired quality in a rapid and efficient manner is provided. Further, a rapid purification method capable of efficiently removing impurities included in the protein composition is provided. Therefore, compared to the conventional purification methods, quality and yield of the protein can be remarkably improved.
    Type: Grant
    Filed: June 28, 2012
    Date of Patent: November 8, 2016
    Inventors: Tsutomu Sugihara, Tomoko Isoda, Yuya Taniguchi, Hidetaka Nomura, Toshiyuki Suzawa
  • Publication number: 20150140595
    Abstract: A set of paramagnetic particles synthesized by co-precipitation methods wherein an alkaline hydroxide solution is mixed with a metal salt solution. The alkaline hydroxide features ammonium hydroxide, potassium hydroxide, sodium hydroxide, or mixtures thereof. The metal salt solution features at least one ferrous salt and at least one tetravalent metal salt selected from Group 4 elements of the Periodic Table. The concentration of the ferrous salt is equal to or greater than the concentration of the tetravalent metal salt. The paramagnetic particles may be used for bioprocessing via magnetic fields. Bioprocessing, for example, may include purifying, concentrating, or detecting biomolecules of interest (e.g., nucleic acids, carbohydrates, peptides, proteins, other organic molecules, cells, organelles, microorganisms, viruses, etc.), or other magnetic field-based processes common to applications in separation science, diagnostics, molecular biology, protein chemistry, and clinical practice.
    Type: Application
    Filed: May 22, 2013
    Publication date: May 21, 2015
    Inventor: Joseph Gerard UTERMOHLEN
  • Publication number: 20150093820
    Abstract: A method of recovering a target from a sample is provided. The method comprises the adding a substrate coupled binding element to the sample comprising the target to form a substrate coupled binding element-target complex; precipitating the complex by changing one or more environmental conditions of the substrate and recovering the target and the substrate coupled binding-element under mild conditions.
    Type: Application
    Filed: October 2, 2013
    Publication date: April 2, 2015
    Applicant: General Electric Company
    Inventors: Anthony John Murray, Radislav Alexandrovich Potyrailo, Andrew David Pris, Nandini Nagraj
  • Publication number: 20150064764
    Abstract: A device for transporting, trapping and escaping a single biomaterial using a magnetic structure, and a method of transporting, trapping and escaping of the single biomaterial using the same are provided, and a method is provided for controlling movement and direction of the single biomaterial including soft magnetic micro structure and magnetic structure in a linear, square storage, apartment type, radial soft magnetic micro structure. Accordingly, the device for transporting, trapping and escaping a single biomaterial and the method for transporting, trapping and escaping single biomaterial using the same can control movement on the lap-on-a-chip with increased precision and ease, by using magnetic force, and thus can be advantageously used in the field of magneto-resistive sensor, or categorization of single cells or biomolecules.
    Type: Application
    Filed: August 29, 2013
    Publication date: March 5, 2015
    Applicant: The Industry & Academic Cooperation in Chungnam National University (IAC)
    Inventors: Cheolgi Kim, Byeonghwa Lim, Reddy Venu, Hu XingHao, KunWoo Kim, Benjamin B. Yellen
  • Patent number: 8951807
    Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution.
    Type: Grant
    Filed: January 13, 2011
    Date of Patent: February 10, 2015
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Larry J. Cummings, Mark A. Snyder
  • Publication number: 20150037867
    Abstract: The invention provides methods for purifying protein products.
    Type: Application
    Filed: February 15, 2013
    Publication date: February 5, 2015
    Inventor: Jacek C. Ostrowski
  • Patent number: 8946395
    Abstract: The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.
    Type: Grant
    Filed: November 12, 2013
    Date of Patent: February 3, 2015
    Assignee: AbbVie Inc.
    Inventors: Matthew Omon Herigstad, Linda E. Rich, Stephen Ming-teh Lu, Natarajan Ramasubramanyan
  • Patent number: 8921518
    Abstract: The present invention provides a method for industrial-scale protein separation by reverse phase chromatography by use of a buffer system and an additional salt.
    Type: Grant
    Filed: December 21, 2006
    Date of Patent: December 30, 2014
    Assignee: Novo Nordisk A/S
    Inventors: Daniel E. Rasmussen, Arne Staby, John Strikart Nielsen, Ole Schou
  • Publication number: 20140363808
    Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.
    Type: Application
    Filed: August 30, 2012
    Publication date: December 11, 2014
    Inventors: Man Bock Gu, Jee Woong Park, Tatavarty Rameshwar
  • Patent number: 8895707
    Abstract: Proteins, including monoclonal antibodies, that have been retained on hydroxyapatite resins for purposes of protein separation, purification, or both, are eluted from the resins by a elution buffer that contains controlled amounts of calcium and phosphate ions. The buffer allows elution to be performed in repeated runs at an acidic pH without deterioration of the resin.
    Type: Grant
    Filed: August 8, 2011
    Date of Patent: November 25, 2014
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Larry J. Cummings
  • Patent number: 8877904
    Abstract: Methods, kits and apparatuses for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by mixed mode chromatography that does not comprise hydroxyapatite (HT) or fluorapatite (FT). The mixed mode chromatography step is then followed by a HT/FT chromatography step.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: November 4, 2014
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Peter S. Gagnon, Hong Chen, Russ Frost
  • Publication number: 20140235837
    Abstract: It is an object of the present invention to provide a method for eluting an adsorbed protein that suppresses a decrease in protein adsorption ability, in a method for purifying a protein using a protein-adsorbing porous membrane. The present invention provides a method for purifying a protein, comprising an adsorption step and an elution step, wherein in the elution step, at least one eluent is passed in the opposite direction with respect to the direction of the passage of a stock solution containing an adsorption target protein, in the adsorption step.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 21, 2014
    Inventors: Yuta Sato, Naoyuki Shinohara, Hironobu Shirataki
  • Publication number: 20140228549
    Abstract: A method to at least one of separate and purify biomolecules via an aqueous two-phase extraction includes providing the biomolecules in a first aqueous phase, providing a porous material comprising pores, providing a second aqueous phase at least in the pores of the porous material, and, at least one of separating and purifying the biomolecules by partially transferring the biomolecules from the first aqueous phase to the second aqueous phase. A dissolution of the biomolecules into the second aqueous phase in the pores of the porous material occurs during the at least one of separating and purifying.
    Type: Application
    Filed: March 27, 2012
    Publication date: August 14, 2014
    Inventors: Gerhard Schembecker, Bernhard Burghoff, Fatma Alexia Van Winssen
  • Publication number: 20140228548
    Abstract: The invention provides methods for separating a polypeptide of interest (such as an antibody) from a virus. In some embodiments, the methods involve eluting the polypeptide of interest from a Protein A resin with an elution buffer have a particular range of conductivity values that minimizes the amount of virus that co-elutes with the polypeptide of interest.
    Type: Application
    Filed: August 31, 2012
    Publication date: August 14, 2014
    Applicant: Human Genome Sciences
    Inventor: Olga Galperina
  • Patent number: 8779110
    Abstract: The invention provides an efficient method of purification of a modified cytokine. The process includes the use of a chromatographic technique for the purification of the desired cytokine. The purified cytokine can be used as a therapeutic composition.
    Type: Grant
    Filed: June 23, 2009
    Date of Patent: July 15, 2014
    Assignees: Dr. Reddy's Laboratories Limited, Dr. Reddy's Laboratories, Inc.
    Inventors: Chaiti Roy, Darshan Koticha, Vivek Arthanari
  • Publication number: 20140187742
    Abstract: The present invention relates to reagents and methods for purifying pertussis toxin (PT).
    Type: Application
    Filed: July 30, 2012
    Publication date: July 3, 2014
    Inventors: Andreas Jungbluth, Eberhard Schneider, Peter Wagner
  • Publication number: 20140178439
    Abstract: A method of preparing a capturing agent for a selected biopolymer or bioparticle, includes the steps of: a) selecting a desired biopolymer or bioparticle, and b) providing a support matrix modified to have, at predetermined conditions, such a net negative surface charge and net surface hydrophobicity in relation to the net negative surface charge and the net surface hydrophobicity of the selected biopolymer or bioparticle that the modified support matrix is capable of selective interaction and capture of the biopolymer or bioparticle through a resulting net hydrophobic interaction being the actual hydrophobic interaction minus the actual electrostatic repulsion. The use of such a capturing agent as a therapeutically active substance, as well as in chromatography and diagnosis are also disclosed.
    Type: Application
    Filed: July 20, 2012
    Publication date: June 26, 2014
    Inventor: Stellan Hjerten
  • Publication number: 20140163209
    Abstract: The present invention relates to a method for preparing a nickel ferrite nanoparticle composite having an inverse spinel structure obtained using a polyol process, a nickel ferrite nanoparticle composite prepared by the method, and a method for selectively binding, separating or purifying a specific protein using the nickel ferrite nanoparticle composite. The method for preparing a magnetic nanoparticle composite according to the present invention includes a one-step hydrothermal synthesis process, and thereby the magnetic nanoparticle composite can be prepared in a simple and economic manner. Also, the nickel ferrite nanoparticles synthesized by the method of the present invention can be strongly magnetic, and also exist in the form of Ni2+ in which Ni binds to a specific protein, thereby preventing loss of separability caused by additional oxidation and repeated recycling of the nanoparticles.
    Type: Application
    Filed: March 29, 2012
    Publication date: June 12, 2014
    Applicant: Postech Academy-Industry Foundation
    Inventors: Gyoo Yeol Jung, Sang Woo Seo, Jinyoung Chun, Jinwoo Lee
  • Publication number: 20140165222
    Abstract: The potential use of these “SUMO receptors” to isolate SUMOylated targets has been considered using the SIM sequences of the SUMO-dependent ubiquitin-protein ligase RNF4. RFN4 contains 4 SIM sequences known to interact with SUMOylated proteins. The capacity of the SIM2 and SIM3 of RNF4 to purify SUMOylated proteins was increased when in the present invention it was disposed in tandem up to 4 SIM sequences. In a preferred embodiment to increase flexibility and functionality of this SUMO-trap, we have changed the natural linkers resulting in a broader capture of SUMOylated proteins. This Tandem SUMO Interacting Motifs (TSIMs) or SUMO-Binding Entities (SUBEs) system disclosed in the present invention is useful to capture polySUMOylated proteins from cell extracts. Therefore, in another embodiment of the present invention, TSIMs or SUBEs can be used for the identification SUMO substrates and the study of SUMO-regulated processes.
    Type: Application
    Filed: March 8, 2012
    Publication date: June 12, 2014
    Inventor: Manuel Salvador Rodriguez Medina
  • Publication number: 20140148410
    Abstract: Disclosed are nucleopeptide compounds that include a nucleobase, and an amino acid. Certain compounds further comprise a glycoside. The compounds may self-assemble to form supramolecular hydrogels. Also, the compounds may be used as a platform to examine specific biological functions (e.g., binding to DNA and RNA) of a dynamic supramolecular system that is able to interact with both proteins and nucleic acids. Other uses include: methods of growing cells and methods of delivering a substance to a cell.
    Type: Application
    Filed: December 2, 2013
    Publication date: May 29, 2014
    Applicant: Brandeis University
    Inventor: Bing Xu
  • Publication number: 20140093937
    Abstract: The present invention provides compositions, methods and kits for the removal of proteins from complex reaction mixtures useful in majority workflows of molecular biology research experiments. More specifically, such compositions, methods and kits are useful in such processes as purification of nucleic acids from biological samples or after their treatment with specific enzymes, when residual enzyme activity in reaction mixture is not compatible with downstream applications.
    Type: Application
    Filed: July 29, 2013
    Publication date: April 3, 2014
    Inventors: Vitalis Tiknius, Egle Merkiene
  • Patent number: 8669354
    Abstract: Method for removal of endotoxin from protein preparations using core-shell nanoparticles, which have the ability to selectively adsorb endotoxin molecules in a protein mixture. The method comprises the steps of (a) preparing a plurality of core-shell nanoparticles; (b) adding the core-shell nanoparticles into a protein preparation containing endotoxin; (c) incubating the core-shell nanoparticles with the protein preparation for a period of time; and (d) separating nanoparticles from the protein preparation.
    Type: Grant
    Filed: June 20, 2011
    Date of Patent: March 11, 2014
    Assignee: The Hong Kong Polytechnic University
    Inventors: Pei Li, Kin Man Ho
  • Publication number: 20140051841
    Abstract: The present invention relates to processes for purifying high-quality recombinant Plasmodium falciparum circumsporozoite protein at high yields.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 20, 2014
    Applicant: PFENEX INC.
    Inventors: Jeffrey Allen, Greg Cantin, Ryan Haverstock
  • Patent number: 8653032
    Abstract: Disclosed is a pharmaceutical composition for preventing or treating TRPV1 activity-related or inflammation-related, diseases or conditions, containing a Maillard peptide separated from well-aged traditional soy sauce as an active ingredient. The Maillard peptide in the present invention functions both as a TRPV1 agonist and a TRPV1 antagonist, and further functions as a TRPV1 activity modulator. Therefore, the Maillard peptide can be used for preventing or treating TRPV1 activity-related diseases such as pain, neurological diseases, urgent defecation, inflammatory bowel disease, respiratory diseases, urinary incontinence, overactive bladder, neurogenic / allergic / inflammatory skin diseases, skin, eye or mucosal irritation, hyperacusis, tinnitus, vestibular hypersensitivity, heart disease, etc.
    Type: Grant
    Filed: November 8, 2010
    Date of Patent: February 18, 2014
    Assignees: Korea Food Research Institute, SNU R & DB Foundation
    Inventors: Mee-Ra Rhyu, Ah-Young Song, Eun-Young Kim, Seog Bae Oh, YoungJoo Lee, Won Chung Lim
  • Patent number: 8648178
    Abstract: The present invention relates to a method for purifying a protein belonging to the TGF-?, superfamily, preferably BMP, and more preferably BMP-2. According to the invention, the number of purification steps is reduced and the purification process is simplified, compared to the conventional BMP-2 purification method. Thus, the time required for purification can be shortened and the cost can be reduced. In addition, the invention solves the problem that as the time for purification increases and the number of purification steps increases, BMP-2 is degraded by protease or lost during purification steps, resulting in a decrease in the final yield of BMP-2. Thus, the invention increases the final yield of BMP-2.
    Type: Grant
    Filed: May 12, 2010
    Date of Patent: February 11, 2014
    Assignee: Korea Bone Bank Co., Ltd.
    Inventors: Young-Bock Shim, Yeong-Schick Kim, Yon-Rak Choi, Ju-Woong Jang
  • Patent number: 8642745
    Abstract: The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method further involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and/or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide.
    Type: Grant
    Filed: December 8, 2006
    Date of Patent: February 4, 2014
    Assignee: Genentech, Inc.
    Inventors: W. Robert Arathoon, Paul J. Carter, Anne M. Merchant, Leonard G. Presta
  • Publication number: 20140004145
    Abstract: Methods of purifying virus-like particles (VLPs) that are substantially free of process contaminants and infectious agents. The methods incorporate, for example, low-pH treatment during harvest and/or inactivation by a solvent and/or detergent during VLP capture.
    Type: Application
    Filed: June 24, 2013
    Publication date: January 2, 2014
    Applicant: Takeda Vaccines (Montana), Inc
    Inventor: Ross TAYLOR
  • Publication number: 20130323812
    Abstract: Methods and compositions are provided for treatment of an apatite-based resin from which retained solutes have been eluted by an elution buffer that contains an alkali metal salt with solutions of calcium ion, phosphate ion, and hydroxide separately from any sample loading and elution buffers. The treatment solutions restore the resin, reversing the deterioration that is caused by the alkali metal salt in the elution buffer.
    Type: Application
    Filed: May 10, 2013
    Publication date: December 5, 2013
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Larry J. Cummings, Jie He
  • Patent number: 8598325
    Abstract: There is described a coated solid phase microextraction (SPME) fiber for use in direct immersion SPME of a food matrix that includes carbohydrates. The coated SPME fiber includes a SPME fiber for absorbing a small molecule from the food matrix; and a protective coating which has a surface that is substantially uniform and substantially smooth, the protective coating reducing adsorption of the carbohydrates onto the SPME fiber and allowing the SPME fiber to extract the small molecule from the food matrix. A process for producing the coated SPME fiber and a method of performing solid phase microextraction are also described.
    Type: Grant
    Filed: March 5, 2012
    Date of Patent: December 3, 2013
    Inventor: Janusz B. Pawliszyn
  • Publication number: 20130317197
    Abstract: The present invention relates to a method for capturing virus-like particles of interest from a mixture comprising the use of an expanded bed of adsorbent; suitably wherein said method comprises the steps of: (a) providing an expanded bed of adsorbent; (b) contacting the mixture with the adsorbent such that the constituents of the mixture contact the expanded bed of adsorbent; (c) optionally washing the adsorbent; and (d) optionally eluting the particle of interest from the adsorbent.
    Type: Application
    Filed: October 27, 2011
    Publication date: November 28, 2013
    Inventors: Allan Lihme, Karen Oishi, Inga Vaarst, Rosa Cabrera
  • Patent number: 8584869
    Abstract: The present invention provides an absorbent which can remove cells present in blood including activated leukocytes such as granulocytes and monocytes, and cancer cells as well as can remove cytokines which facilitate the activation of the remaining cells, and further has no concern for pressure loss and has high configuration stability. That is, the present invention provides an absorbent which absorbs the granulocytes and the monocytes in blood, an absorbent for cancer therapy which absorbs an immunosuppressive protein and an absorbent having a bilayer structure of a net and a nonwoven fabric, having a zeta potential of ?20 mV or more, as well as a blood circulation column containing any of the absorbents filled therein.
    Type: Grant
    Filed: March 31, 2006
    Date of Patent: November 19, 2013
    Assignee: Toray Industries, Inc.
    Inventors: Masaaki Shimagaki, Yasufumi Yamamura, Katsuhisa Sato, Kazuo Teramoto, Takeshige Oozeki, Shigehisa Wada
  • Patent number: 8563701
    Abstract: This disclosure relates to methods of removing contaminating microcystins toxins from preparations of blue-green algae. It also relates to methods of purifying phycocyanin from blue-green algae extracts.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: October 22, 2013
    Assignee: Desert Lake Technologies, LLC
    Inventors: Axel Ehmann, Jesse Guthrie
  • Patent number: 8497358
    Abstract: Provided is a purification method that purifies an antibody to a high purity, effectively removes antibody polymer (or aggregate), and improves antibody recovery rate. An antibody purification method including a step for treating a solution containing an antibody by mixed mode chromatography in the presence of an amino acid is provided.
    Type: Grant
    Filed: December 18, 2009
    Date of Patent: July 30, 2013
    Assignee: Takeda Pharmaceutical Company Limited
    Inventors: Masato Suenaga, Takeshi Hayakawa, Takuya Muramoto
  • Publication number: 20130190482
    Abstract: Helicobacter pylori is closely associated with chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma. Helicobacter pylori neutrophil-activating protein (HP-NAP), a virulence factor of Helicobacter pylori, plays an important role in pathogenesis of Helicobacter pylori infection. Since HP-NAP has been proposed as a candidate vaccine against Helicobacter pylori infection, an efficient way to obtain pure HP-NAP needs to be developed. In the present invention, recombinant HP-NAP expressed in Bacillus subtilis and Escherichia coli was purified through a single step of DEAE Sephadex ion-exchange chromatography with high purity. Also, purified recombinant HP-NAP was able to stimulate neutrophils to produce reactive oxygen species. Thus, recombinant HP-NAP obtained from our Bacillus subtilis expression system and Escherichia coli expression system is functionally active.
    Type: Application
    Filed: July 27, 2012
    Publication date: July 25, 2013
    Inventors: Hua-Wen Fu, Kuo-Shun Shih, Chih-Chang Lin, Yu-Chi Yang
  • Patent number: 8481693
    Abstract: Methods for purifying human Calciviruses are disclosed, including Noroviruses and Sapoviruses.
    Type: Grant
    Filed: March 14, 2008
    Date of Patent: July 9, 2013
    Assignee: Takeda Vaccines (Montana), Inc.
    Inventors: Thomas S. Vedvick, Bryan Steadman, Charles Richardson, Thomas R. Foubert, Charles R. Petrie
  • Patent number: 8470981
    Abstract: The invention relates to a method of dewatering corn gluten stream wherein coagulant is add to the corn gluten stream of the corn wet milling process. The method of dewatering corn gluten uses an effective amount of a coagulant of one or more anionic polymers, the anionic polymers comprising one or more anionic monomers. The method further includes a process for separating the water from the gluten using a solids/liquids filtration device.
    Type: Grant
    Filed: June 29, 2007
    Date of Patent: June 25, 2013
    Assignee: Nalco Company
    Inventors: David W. Scheimann, Angela S. Kowalski
  • Publication number: 20130158240
    Abstract: A method is provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The method includes the steps of capturing the fraction-bound solid phase substrate and bringing an isolation buffer and the fraction-bound solid phase substrate into contact to purify the captured fraction-bound solid phase substrate.
    Type: Application
    Filed: December 15, 2011
    Publication date: June 20, 2013
    Inventors: David Beebe, Lindsay Strolman, Scott M. Berry
  • Patent number: 8461302
    Abstract: The present invention provides improved methods for the purification of factor XIII. In particular, the methods provide compositions containing 5% or less contaminating proteins. In particular embodiments of the present invention the methods provide purified factor XIII compositions comprising less than 1% activated factor XIII, less than 2% protein aggregates, and/or less than 5% charge isomers of factor XIII. The methods do not require the use a precipitation or crystallization step common to prior methods of isolating factor XIII. Instead, the method uses immobilized metal affinity chromatography to remove various contaminants common to recombinant expression of factor XIII. Further, a combination of various chromatography methods including ion exchange chromatography, hydrophobic affinity chromatography, and immobilized metal affinity chromatography comprise a simple and less expensive method to produce a pharmaceutical grade factor XIII product at high yield.
    Type: Grant
    Filed: November 23, 2005
    Date of Patent: June 11, 2013
    Assignee: Zymogenetics, Inc.
    Inventors: Carol Jewell, Hardarshan Cheema, Deborah Hogg, Wenmao Meng, Ray O'Donnell, Ewan Robertson, Andrew Topping
  • Publication number: 20130096210
    Abstract: The disclosure relates to metal organic frameworks or isoreticular metal organic frameworks, methods of production thereof, and methods of use thereof.
    Type: Application
    Filed: October 12, 2012
    Publication date: April 18, 2013
  • Patent number: 8394933
    Abstract: It is an object of the present invention to provide: a protein refolding column filler, which is effective for the refolding, namely, the activation of the function, of an inactive protein with an as yet unformed higher order structure produced in Escherichia coli or the like, or a protein whose conformation has been changed due to a certain cause and which has become inactivated; and a column filled with the aforementioned column filler. The present invention provides a protein refolding column filler, which comprises zeolite with BEA structure (Zeolite Beta) that is granulated into a particle state.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: March 12, 2013
    Assignee: FUJIFILM Corporation
    Inventors: Masayuki Kawakami, Tatsuo Tsunoda, Hideaki Togashi, Takayuki Nara, Shun-ichi Matsuura, Chisato Sekikawa, Akiko Kawai, Akiyoshi Kawata, Fujio Mizukami, Kengo Sakaguchi
  • Publication number: 20130048853
    Abstract: An adsorbent that enables solid phase extraction with high efficiency and good selectivity of solutes having a broad chromatographic polarity range including high-polarity solute molecules while suppressing adsorption of impurities, and a solid phase extraction method therefor are provided. A heterocyclic-ring-containing copolymer adsorbent that is provided herein comprises a copolymer that comprises: a multifunctional heterocyclic-ring-containing monomer having a heterocyclic ring containing at least two heteroatoms in the ring system; and a monomer that is copolymerizable with the multifunctional heterocyclic-ring-containing monomer, wherein the multifunctional heterocyclic ring constitutes the main chain structure.
    Type: Application
    Filed: April 28, 2011
    Publication date: February 28, 2013
    Inventors: Jun Nunoshige, Hiroyuki Kagawa, Shinya Ito, Katsuhiro Kanda, Izumi Waki, Kotaro Araya, Terumi Tamura
  • Patent number: 8383782
    Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.
    Type: Grant
    Filed: October 30, 2009
    Date of Patent: February 26, 2013
    Assignee: Natrix Separations Inc.
    Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
  • Patent number: 8383776
    Abstract: The invention relates to a method for purifying a factor XIII polypeptide from a biological material, the method comprising subjecting the material to sequential chromatography on an anion-exchange matrix and a hydrophobic interaction matrix.
    Type: Grant
    Filed: July 19, 2010
    Date of Patent: February 26, 2013
    Assignee: Novo Nordisk Health Care AG
    Inventor: Susanne Bang
  • Patent number: 8362219
    Abstract: The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. In some embodiments, the analyte is a protein. The invention further provides reagents, channels, columns and instrumentation related to this and other methods.
    Type: Grant
    Filed: January 11, 2010
    Date of Patent: January 29, 2013
    Assignee: PhyNexus, Inc.
    Inventors: Douglas T. Gjerde, Christopher P. Hanna
  • Publication number: 20130005950
    Abstract: Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.
    Type: Application
    Filed: September 12, 2012
    Publication date: January 3, 2013
    Inventors: Wilson Moya, Alison Dupont
  • Publication number: 20120232254
    Abstract: Method for removal of endotoxin from protein preparations using core-shell nanoparticles, which have the ability to selectively adsorb endotoxin molecules in a protein mixture. The method comprises the steps of (a) preparing a plurality of core-shell nanoparticles; (b) adding the core-shell nanoparticles into a protein preparation containing endotoxin; (c) incubating the core-shell nanoparticles with the protein preparation for a period of time; and (d) separating nanoparticles from the protein preparation.
    Type: Application
    Filed: June 20, 2011
    Publication date: September 13, 2012
    Inventors: Pei Li, Kim Man Ho
  • Publication number: 20120190619
    Abstract: A mammalian C-type lectin receptor type is identified which is shown to bind IgG antibodies or Fc fragments, thus inducing WIG-related reversal of inflammation associated with various immune disorders. The identification of a DC-SIGN receptor type which interacts with IgG to promote a biological response reducing inflammation associated with immune disorders provides for methods of screening and selecting compounds which may be useful in treating various immune disorders by acting to modulate a DC-SIGN(+) cell to signal a second effector macrophage, causing an increase in expression of the Fc?RIIB receptor and in turn inhibiting a cellular-mediated inflammatory response.
    Type: Application
    Filed: September 8, 2010
    Publication date: July 26, 2012
    Inventors: Jeffrey V. Ravetch, Robert Anthony
  • Publication number: 20120190579
    Abstract: The present invention relates to a product comprising a solid substrate and a moiety of formula (I) linked thereto: wherein X, X? and R are as defined herein. The product is useful for immobilising target molecules such as molecules of biochemical interest to solid substrates for numerous applications, such as affinity chromatography, ELISA, biotechnological assay techniques and solid phase peptide synthesis.
    Type: Application
    Filed: August 9, 2010
    Publication date: July 26, 2012
    Applicant: UCL Business PLC
    Inventors: Mark Smith, Stephen Caddick, James Baker
  • Patent number: 8227599
    Abstract: A process for the separation of biological materials, such as dye-ligand affinity chromatography, wherein an adsorbent is used, which comprises a reaction product of certain reactive anthroquinone compounds and a substrate having a group capable of reaction with a reactive group in said reactive anthroquinone compounds to form a covalent bond.
    Type: Grant
    Filed: March 29, 2006
    Date of Patent: July 24, 2012
    Assignee: BASF SE
    Inventors: Vadiraj Subbanna Ekkundi, Narayan S. Punekar, Kamalesh Pai Fondekar, Georg Roentgen, Shilpa S. Korde, Ajit B. Shinde, Masana Moorthy