Inorganic Patents (Class 530/420)
  • Patent number: 8835612
    Abstract: The invention provides methods and apparatus for the selective isolation of phosphorylated and glycosylated proteins and their fragments. Metal cation is used to precipitate proteins or protein fragments containing phospho groups and/or glyco groups. The sample preparation method can be used for several types of biological samples, including HeLa cells, food, and human cerebrospinal fluid. The proteins are isolated, recovered and ready for analysis by mass spectrometry or other analytical methods allowing detection limits down to the femtomole level. The method and apparatus are valuable tools in the field of protein analysis and diagnostics.
    Type: Grant
    Filed: October 10, 2011
    Date of Patent: September 16, 2014
    Inventors: Fabio Polato, Mathias Rainer, Douglas T. Gjerde, Guenther Bonn
  • Patent number: 8791231
    Abstract: Disclosed herein are compounds useful in the preparation of non-natural amino acids and polypeptides that include at least one non-natural amino acid, and methods for making such non-natural amino acids and polypeptides. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of possible functionalities, but typical have at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Also disclosed herein are compounds useful in the preparation of non-natural amino acid polypeptides that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such resultant polypeptides. Typically, the modified non-natural amino acid polypeptides include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid polypeptides and modified non-natural amino acid polypeptides, including therapeutic, diagnostic, and other biotechnology uses.
    Type: Grant
    Filed: January 13, 2011
    Date of Patent: July 29, 2014
    Assignee: Ambrx, Inc.
    Inventors: Zhenwei Miao, Junjie Liu, Thea Norman, Russell Driver
  • Publication number: 20130045544
    Abstract: The present invention related to an improved method for the detection of prion proteins, especially abnormal prion proteins (PrPSc), in samples of blood, such as plasma. The invention also relates to kits for use in such methods.
    Type: Application
    Filed: May 3, 2011
    Publication date: February 21, 2013
    Applicant: Common Services Agency
    Inventor: Michael Jones
  • Patent number: 8133521
    Abstract: The present invention relates to methods for the separation of various components from eggs. More particularly, the present invention relates to methods for the separation of proteins and lipids from eggs, including technical eggs (inedible) or edible eggs, yolks or whites, which comprises cross-linking the lipids of eggs with a cross-linking reagent. In an embodiment, the method includes separating the proteins from the cross-linked lipids. In an embodiment, the method includes the separation of various components associated with the cross-linked lipids. The methods disclosed herein allow for the isolation of multiple different components from the egg in an efficient, cost-effective manner without compromising the recovery process of the components or their subsequent utility in various applications or compositions. The compositions and isolated components obtained by the methods of the invention can be used in pharmaceutical, medical, nutritional, cosmetic or health applications.
    Type: Grant
    Filed: January 9, 2008
    Date of Patent: March 13, 2012
    Assignee: Biova, L.L.C.
    Inventors: Ronald E. Strohbehn, Jesse I. Figgins
  • Patent number: 8124743
    Abstract: The present invention discloses a method of purifying bivalent antibodies or antibody fragments that are active at both Fab sites from a source of antibodies or antibody fragments using a non-chromatographic method that includes inducing the formation of cyclic immunoglobulin aggregates by addition of multivalent hapten to a salt solution of soluble antibodies or antibody fragments, wherein the multivalent hapten possesses a linker between the two haptens effective to prevent the binding of both haptens of the ligand to the same antibody or antibody fragment.
    Type: Grant
    Filed: June 1, 2007
    Date of Patent: February 28, 2012
    Assignee: President and Fellows of Harvard College
    Inventors: Vijay M. Krishnamurthy, Lara A. Estroff, Vincent Semetey, Samuel W. Thomas, George K. Kaufman, Zihni Basar Bilgicer, George M. Whitesides
  • Patent number: 8080272
    Abstract: The present invention relates to methods for the separation of various components from eggs. More particularly, the present invention relates to methods for the separation of proteins and lipids from eggs, including technical eggs (inedible) or edible eggs, yolks or whites, which comprises cross-linking the lipids of eggs with a cross-linking reagent. In an embodiment, the method includes separating the proteins from the cross-linked lipids. In an embodiment, the method includes the separation of various components associated with the cross-linked lipids. The methods disclosed herein allow for the isolation of multiple different components from the egg in an efficient, cost-effective manner without compromising the recovery process of the components or their subsequent utility in various applications or compositions. The compositions and isolated components obtained by the methods of the invention can be used in pharmaceutical, medical, nutritional, cosmetic or health applications.
    Type: Grant
    Filed: January 9, 2008
    Date of Patent: December 20, 2011
    Assignee: Biova, L.L.C.
    Inventors: Ronald E. Strohbehn, Jesse I. Figgins
  • Patent number: 7943746
    Abstract: The invention relates to a process for the purification of IL-18 binding protein (IL-18BP) from a fluid using aqueous two-phase partitioning.
    Type: Grant
    Filed: October 13, 2008
    Date of Patent: May 17, 2011
    Assignee: Ares Trading S.A.
    Inventors: Henri Kornmann, Gianni Baer
  • Patent number: 7879800
    Abstract: A process suitable for processing scaled-up amounts of source material in the range of tens of kilograms for the purification of alpha-1 proteinase inhibitor (API) from a mixture of unpurified proteins is provided. More particularly, a process for the purification of API from blood plasma or from plasma fractions to obtain pharmaceutical grade API on a commercial scale is provided. The API produced by the process is highly pure (at least 90% API out of the total protein) and highly active (at least 90% active API). Pharmaceutical compositions comprising the purified API and methods of using same are also described.
    Type: Grant
    Filed: September 22, 2004
    Date of Patent: February 1, 2011
    Assignee: Kamada Ltd.
    Inventor: Shabtai Bauer
  • Patent number: 7820801
    Abstract: A method for producing solid proteinase K which is insoluble in water and has high purity, the method comprising the steps of adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to a final amount of 1.5 to 2 M after 1 hour, thereby precipitating the proteinase K as a solid, and isolating the solid proteinase K.
    Type: Grant
    Filed: September 29, 2006
    Date of Patent: October 26, 2010
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Helmut Schoen, Guenter Guertler, Bernhard Rexer, Johann-Peter Thalhofer
  • Patent number: 7601515
    Abstract: A process is provided for the preparation of albumin from a yeast culture medium which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises separating the yeast from the culture medium to yield an albumin solution; subjecting the albumin solution to positive mode cation exchange, affinity, and positive mode anion exchange chromatography; and concentrating and formulating the albumin with sodium octanoate and sodium chloride.
    Type: Grant
    Filed: April 23, 2007
    Date of Patent: October 13, 2009
    Assignee: Novozymes Biopharma UK Limited
    Inventors: Andrew Robert Goodey, Darell Sleep, Hendrik Van Urk, Stephen Berezenko, John Rodney Woodrow, Richard Alan Johnson, Patricia Carol Wood, Stephen James Burton, Alan Victor Quirk
  • Patent number: 7498412
    Abstract: The subject invention provides a process for preparing a porous collagen matrix from connective tissue, said process comprising: a porous structure forming step to treat said connective tissue with poring agent in situ; and a washing step to remove the impurity from said porous connective tissue thereby obtaining a porous collagen matrix.
    Type: Grant
    Filed: March 8, 2006
    Date of Patent: March 3, 2009
    Inventors: Lynn L. H. Huang, Gin-Mol Liu
  • Patent number: 7439336
    Abstract: The invention relates to a process for the purification of IL-18 binding protein (IL-18BP) from a fluid using aqueous two-phase partitioning.
    Type: Grant
    Filed: June 27, 2005
    Date of Patent: October 21, 2008
    Assignee: Ares Trading S.A.
    Inventors: Henri Kornmann, Gianni Baer
  • Publication number: 20080139465
    Abstract: A process suitable for processing scaled-up amounts of source material in the range of tens of kilograms for the purification of alpha-1 proteinase inhibitor (API) from a mixture of unpurified proteins is provided. More particularly, a process for the purification of API from blood plasma or from plasma fractions to obtain pharmaceutical grade API on a commercial scale is provided. The API produced by the process is highly pure (at least 90% API out of the total protein) and highly active (at least 90% active API). Pharmaceutical compositions comprising the purified API and methods of using same are also described.
    Type: Application
    Filed: June 23, 2005
    Publication date: June 12, 2008
    Applicant: KAMADA LTD.
    Inventor: Shabtai Bauer
  • Patent number: 7381789
    Abstract: Methods for forming repeat protein polymers and utilizing the repeat protein polymers to form inorganic structures are provided. The inorganic structures may have features on the nanoscale, and the structures generally do not have the repeat protein polymer incorporated therein.
    Type: Grant
    Filed: May 20, 2003
    Date of Patent: June 3, 2008
    Assignee: Genencor International, Inc.
    Inventor: Manoj Kumar
  • Patent number: 7262058
    Abstract: The amount of lipid associated sialoprotein (LSP) in body fluids such as cerebrospinal fluid, peritoneal fluid, pleural fluid, bronchial washings, saliva and sputum samples, may be determined by a method which may be automated, involving the following steps to be performed on the sample: adding a mixture of a chlorinated lower alkyl alcohol; centrifuging to yield a substantially clear upper phase; recovering the upper phase and adding to it a protein precipitating agent; mixing the resulting admixture; recovering the resulting precipitate; washing the precipitate with saline solution; centrifuging to recover the precipitate; dissolving the precipitate in water; mixing; adding to the resulting mixture an hydrolysis agent; heating; and determining the amount of lipid associated sialoprotein present by determining the optical density of the sample.
    Type: Grant
    Filed: March 9, 2001
    Date of Patent: August 28, 2007
    Inventor: Nonda Katopodis
  • Patent number: 7244828
    Abstract: A method of protein precipitation, concentration and removal of non-protein agents from the protein solution wherein the protein solution is treated with a protein-precipitation agent containing an acidic agent, a salt and a precipitate forming agent. After precipitation, the protein precipitate is washed with a water miscible organic solvent agent to remove non-protein agents present in the protein precipitate.
    Type: Grant
    Filed: February 3, 2005
    Date of Patent: July 17, 2007
    Inventor: Aftab Alam
  • Patent number: 7232894
    Abstract: Methods for the production of recombinant peptides comprise fermenting cells to produce recombinant peptides. A metal salt is added to a concentrate of the fermented cells after the fermentation step, prior to peptide isolation, and the pH of the cell concentrate after the addition of the metal salt is less than or equal to 7, thereby reducing the amount of trifulsides formed in the production of the recombinant peptide, with the proviso that the production does not include conversion of formed trifulside peptide into native peptide form after the addition of the metal salt. In one embodiment, the recombinant peptide is recombinant growth hormone.
    Type: Grant
    Filed: July 5, 1999
    Date of Patent: June 19, 2007
    Assignee: Pharmacia AB
    Inventors: Barbro Hemmendorff, Andreas Castan, Anders Persson
  • Patent number: 7223561
    Abstract: A process is provided for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through positive mode cation exchange and then positive mode anion exchange chromatography. Other steps may also be employed, ultrafiltration, gel permeation chromatography, affinity chromatography binding the albumin by using blue dyes) and affinity chromatography binding contaminants by using an aminophenylboronic acid resin. Elution of albumin, with a compound having affinity for albumin, from a material having no specific affinity for albumin is also disclosed, as is removal of ammonium ions with a counter-ion.
    Type: Grant
    Filed: June 22, 2004
    Date of Patent: May 29, 2007
    Assignee: Novozymes Delta, Limited
    Inventors: Andrew Robert Goodey, Darell Sleep, Hendrik Van Urk, Stephen Berezenko, John Rodney Woodrow, Richard Alan Johnson, Patricia Carol Wood, Stephen James Burton, Alan Victor Quirk
  • Patent number: 6998251
    Abstract: Reactors and methods for miniaturized reactions having enhanced reaction kinetics. In particular the subject matter is directed to chemical and biological reactions conducted in a nanoporous membrane environment. The subject matter contemplates methods for modifying the kinetics of reactions and devices for conducting reactions having modified kinetics. The subject matter also provides systems for rapid miniaturized reactions. Further the subject matter includes methods and kits for conducting a reaction with enhanced throughput and methods of conducting miniaturized, high throughput analyses of reaction products, and the like. Reactions performed on or within a nanoporous membrane exhibits improved kinetic characteristics.
    Type: Grant
    Filed: January 14, 2002
    Date of Patent: February 14, 2006
    Assignee: Syngenta Participations AG
    Inventors: Andras Guttman, Zsolt Ronai, Csaba Barta
  • Patent number: 6967239
    Abstract: Method for preparing a factor VIII solution that is essentially free of viruses and essentially devoid of vWF (von Willebrand factor) and factor VIII-vWF complexes by (a) obtaining a starting factor VIII solution devoid of factor VIII-vWF complexes; and (b) filtering the solution through a hydrophilic virus filter.
    Type: Grant
    Filed: December 14, 1998
    Date of Patent: November 22, 2005
    Assignee: Laboratoire Francias du Franctionnement et des Biotechnologies
    Inventors: Abdessatar Chtourou, Michel Nogre, Pierre Porte
  • Patent number: 6894023
    Abstract: A process for producing cation crystals of growth hormone or growth hormone derivatives, as well as growth hormone and growth hormone derivatives. The process of producing the growth hormone crystals includes the steps of (a) adding cations of inorganic or organic nature and an organic solvent or a mixture of inorganic solvents at a pH between 5.0 and 6.8 to a solution of growth hormone or derivatives. (b) growing growth hormone crystals at a temperature from about 0 to 30° C. and (c) isolating the cation crystals.
    Type: Grant
    Filed: June 12, 2000
    Date of Patent: May 17, 2005
    Assignee: Novo Nordisk A/S
    Inventors: Flemming Junker, Claus Friis Theisen
  • Patent number: 6875617
    Abstract: A method of protein precipitation, concentration and removal of non-protein agents from the protein solution wherein the protein solution is treated with a protein-precipitation agent containing an acidic agent, a salt and a precipitate forming agent. After precipitation, the protein precipitate is washed with a water miscible organic solvent agent to remove non-protein agents present in the protein precipitate.
    Type: Grant
    Filed: April 27, 2001
    Date of Patent: April 5, 2005
    Assignee: Geno Technology, Inc.
    Inventor: Aftab Alam
  • Patent number: 6777538
    Abstract: A 24 kd protein capable of binding the E2 envelope protein of hepatitis C virus (HCV), and functionally equivalent variants or fragments of the 24 kd protein, are disclosed. Processes for production and purification of the 24 kd protein, and functionally equivalent variants or fragments thereof, are also disclosed.
    Type: Grant
    Filed: January 5, 2001
    Date of Patent: August 17, 2004
    Assignee: Chiron S.r.l.
    Inventor: Sergio Abrignani
  • Patent number: 6759384
    Abstract: The invention provides compounds which specifically inhibit factor Xa activity having the structure A1—A2—(A3)m—B, where m is 0 or 1. A compound of the invention is characterized, in part, in that it exhibits a specific inhibition of factor Xa activity with a Ki≦100 &mgr;M, preferably ≦2 nM, and does not substantially inhibit the activity of other proteases involved in the coagulation cascade. The invention further provides methods of specifically inhibiting the activity of factor Xa and of inhibiting blood clotting in vitro and in an individual and methods of detecting factor Xa levels or activity.
    Type: Grant
    Filed: December 14, 1998
    Date of Patent: July 6, 2004
    Assignee: Aventis Pharmaceuticals Inc.
    Inventors: Fahad Al-Obeidi, Michal Lebl, James A. Ostrem, Pavel Safar, Alena Stierandova, Peter Strop, Armin Walser
  • Patent number: 6756354
    Abstract: The present invention provides biotechnologically useful oligo(ethylene glycol)-terminated 1,2-dithiolane compositions and conjugates of these compositions with biological or non-biological receptor, ligand, sequestering, or reporter moieties to provide physiologically active therapeutic compositions. The invention also provides methods for the preparation of these compositions. Further, the invention provides self-assembled monolayer (SAM) compositions on a metal and methods for their preparation.
    Type: Grant
    Filed: April 3, 2002
    Date of Patent: June 29, 2004
    Inventor: Deanna Jean Nelson
  • Patent number: 6737233
    Abstract: A 24 kd percent capable of binding the E2 envelope protein of hepatitis C virus (HCV), and functionally equivalent variants or fragments of the 24 kd protein, are disclosed. Processes for production and purication of the 24 kd protein, and functionally equivalent variants or fragments thereof, are also disclosed.
    Type: Grant
    Filed: February 17, 1998
    Date of Patent: May 18, 2004
    Assignee: Chiron S.r.l.
    Inventor: Sergio Abrignani
  • Patent number: 6676840
    Abstract: The precipitation and removal of suspended fat and protein components and other suspended solids from a given solution can be accomplished by the addition of an aluminate to complex with the fat and protein components and the addition of a flocculating agent to flocculate the aluminate/fat and protein component complexes into insoluble particles capable of removal by conventional technology, which insoluble particles can be rendered to produce tallow.
    Type: Grant
    Filed: March 22, 2002
    Date of Patent: January 13, 2004
    Assignee: Power Engineering Company
    Inventors: Bryon J. Tarbet, Robert D. Hancock, Jeffrey W. Zidek
  • Patent number: 6638740
    Abstract: The present invention relates to a process for purifying the protein human serum albumin extracted from serum or recombinant human albumin produced by transforming a microorganism with a nucleotide coding sequence encoding the amino acid sequence of human serum albumin. The present invention provides highly purified albumin.
    Type: Grant
    Filed: February 24, 1999
    Date of Patent: October 28, 2003
    Assignee: Delta Biotechnology Ltd.
    Inventors: Andrew Robert Goodey, Darrell Sleep, Hendrik Van Urk, Stephen Berezenko, John Rodney Woodrow, Richard Alan Johnson, Patricia Carol Wood, Stephen James Burton, Alan Victor Quirk
  • Patent number: 6635746
    Abstract: Immunogenic compositions including vaccines are described that comprise an outer membrane antigen extract of a strain of Chlamydia and are effective in protection against disease caused by Chlamydia infection The immunogenic compositions may comprise the major outer membrane protein (MOMP) of Chlamydia which may be in a homooligomeric form or complexed with at least one other antigen of Chlamydia. The immunogenic composition may include an immunostimulating complex (ISCOM) and the outer membrane antigen may be incorporated therein. The immunogenic compositions have utility as chlamydial vaccines and in diagnostic applications.
    Type: Grant
    Filed: May 28, 1999
    Date of Patent: October 21, 2003
    Assignee: Aventis Pasteur Limited
    Inventors: Andrew D. Murdin, Brian J. Underdown
  • Patent number: 6610630
    Abstract: A pseudobioaffinity chromatography adsorbent adapted for use in selectively adsorbing immunoglobulins. In one embodiment, the adsorbent includes: (a) a solid support material; and (b) a ligand immobilized on the surface of the solid support material, said ligand being a compound of the formula wherein Y1 is selected from the group consisting of S, SCH3+, O, NH, NCH3, CH2 and CR1R2 wherein at least one of R1 and R2 is not hydrogen; wherein each of Y2, Y3 and Y4 is selected from the group consisting of N, NCH3+, CH, and CR wherein R is not hydrogen; and wherein at least two of Y1, Y2, Y3 and Y4 are neither CH2, CH, CR nor CR1R2.
    Type: Grant
    Filed: February 13, 1998
    Date of Patent: August 26, 2003
    Assignee: Ciphergen Biosystems, Inc.
    Inventors: Alexander Schwarz, Meir Wilchek
  • Publication number: 20030149249
    Abstract: A process for isolation of milk osteopontin from a material containing milk osteopontin by optionally mixing the milk material with a calcium source and separate the osteopontin containing phase from the rest of the milk material by pH adjustment.
    Type: Application
    Filed: November 12, 2002
    Publication date: August 7, 2003
    Inventors: Esben Skipper Sorensen, Steen Ostersen, Dereck Chatterton, Hans Henrick Holst, Kristian Albertsen
  • Patent number: 6566329
    Abstract: A readily-soluble freeze-dried solid preparation of hGH with a minimal content of degradation products in terms of deamidation, dimers, polymers, and sulphoxide forms, obtainable by a method comprising a single lyophilization of an aqueous slurry of an amorphous hGH isoprecipitate, the slurry having a pH of from about 4.7 to 5.0 and being essentially free of buffer components other than acetate.
    Type: Grant
    Filed: June 14, 2000
    Date of Patent: May 20, 2003
    Assignee: Novo Nordisk A/S
    Inventors: Giorgio Meyn, Hans Holmegaard Sørensen, Thorkild Christensen
  • Publication number: 20030078392
    Abstract: A method for diaggregating and reforming the casein micelles of milk to produce a product with physical properties differing significantly from that of the original milk. There is also provided a milk fraction highly enriched in beta-lactoglobulin (BLG) and a soluble whey fraction correspondingly depleted.
    Type: Application
    Filed: October 15, 2002
    Publication date: April 24, 2003
    Inventors: Jeff Leaver, Andrew Law
  • Patent number: 6461604
    Abstract: Crystallographic and NMR solution structures of human IL-6 are reported. The invention provides models and systems incorporating such structures which are useful for identifying IL-6/IL-6 receptor interactions and for identification of agonists and antagonists of such interactions. Crystalline human IL-6 is also provided.
    Type: Grant
    Filed: February 7, 2000
    Date of Patent: October 8, 2002
    Assignee: Genetics Institute, LLC
    Inventors: William S. Somers, Mark L. Stahl, Jasbir S. Seehra, Guang-Yi Xu, Thomas E. McDonagh, Hsiang-Ai Yu, Jin Hong
  • Patent number: 6448374
    Abstract: A method for the preparation of selected phosphopeptides having anticariogenic and other activities, comprising the steps of completely digesting a soluble monovalent cation salt of casein in solution with a proteolytic enzyme, adding a mineral acid to the solution to adjust the pH to about 4.7, removing any precipitate produced, adding CaCl2 to a level of about 1.0% w/v to cause aggregation of at least the selected phosphopeptides in said digested solution, separating the aggregated phosphopeptides from the solution through a filter having a molecular weight exclusion limit lying substantially within the range 10,000 to 20,000 while passing the bulk of the remaining phosphopeptides and solution, diafiltering the separated phosphopeptides with water through a filter and concentrating and drying the retentate.
    Type: Grant
    Filed: March 4, 1994
    Date of Patent: September 10, 2002
    Assignee: The University of Melbourne
    Inventor: Eric Charles Reynolds
  • Patent number: 6444211
    Abstract: Pertactin (formerly 69 kDa protein) is recovered in stable biologically pure form having no detectable adenylate cyclase activity from fermentation broth from the fermentation of Bordetella pertussis as well as from the cells. The broth is processed to selectively remove pertussis toxin (PT) and filamentous haemagglutinin (FHA), the pertactin is precipitated by ammonium sulphate and the precipitate is dissolved in buffer at pH 6.0 to 8.5, the solution then is passed through hydroxyapatite and ion-exchange chromatograph columns before final ultrafiltration. Cells are extracted with urea and the extract ultrafiltered and diafiltered. The pertactin is precipitated from the extract and the precipitate processed as above. In a variation, the broth is contacted with ammonium sulphate to precipitate pertactin, PT and FHA, the precipitate is dissolved and the PT and FHA selectively removed, before the solution is passed to the chromatograph columns.
    Type: Grant
    Filed: June 8, 1999
    Date of Patent: September 3, 2002
    Assignee: Connaught Laboratories, Inc.
    Inventors: Gail Jackson, Raafat Fahim, Larry Tan, Pele Chong, John Vose, Michel Klein
  • Patent number: 6437101
    Abstract: Methods are provided in this invention for the isolation of human growth hormone, growth hormone antagonist, or a homologue of either, from a biological source. The methods of the invention use multi-phase extraction.
    Type: Grant
    Filed: May 7, 1999
    Date of Patent: August 20, 2002
    Assignee: Akzo Nobel N.V.
    Inventors: Kirk James Hayenga, Pascal P. Valex
  • Patent number: 6372145
    Abstract: The precipitation and removal of suspended fat and protein components and other suspended solids from a given solution can be accomplished by the addition of an aluminate to complex with the fat and protein components and the addition of a flocculating agent to flocculate the aluminate/fat and protein component complexes into insoluble particles capable of removal by conventional technology, which insoluble particles can be rendered to produce tallow.
    Type: Grant
    Filed: June 5, 2000
    Date of Patent: April 16, 2002
    Assignee: Power Engineering Company
    Inventors: Bryon J. Tarbet, Robert D. Hancock, Jeffrey W. Zidek
  • Patent number: 6335430
    Abstract: The invention relates to a process of producing MAP-products from mussel feet. The process is characterised by extracting the mussel feet in a weakly acid aqueous solution containing, for instance, 1-10 percent by weight of a weak acid and 0.5-3 percent by weight of perchloric acid, whereafter the proteins in the aqueous solution are precipitated by adding inorganic or organic salts and separated from the system, subsequent to having extracted the solid substances.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: January 1, 2002
    Inventor: Magnus Qvist
  • Publication number: 20010034066
    Abstract: A method of protein precipitation, concentration and removal of non-protein agents from the protein solution wherein the protein solution is treated with a protein-precipitation agent containing an acidic agent, a salt and a precipitate forming agent. After precipitation, the protein precipitate is washed with a water miscible organic solvent agent to remove non-protein agents present in the protein precipitate.
    Type: Application
    Filed: April 27, 2001
    Publication date: October 25, 2001
    Inventor: Aftab Alam
  • Patent number: 6284874
    Abstract: The present invention is directed to a process for purifying &agr;1-proteinase inhibitor. The process comprises providing an impure protein fraction which comprises &agr;1-proteinase inhibitor. The impure protein fraction is precipitated with a precipitant comprising PEG. The supernatant from the PEG precipitation, which comprises &agr;1-proteinase inhibitor, is collected and applied to an anion-exchange medium. A fraction comprising &agr;1-proteinase inhibitor is recovered from the anion-exchange medium and applied to a metal chelate medium. A fraction comprising &agr;1-proteinase inhibitor is then recovered from the metal chelate medium. Alpha1-proteinase inhibitor purified by the process has a specific activity greater than 0.6 units/mg.
    Type: Grant
    Filed: June 17, 1994
    Date of Patent: September 4, 2001
    Assignee: Alpha Therapeutic Corporation
    Inventors: T. (Tom) Taniguchi, John M. Rolf, Prabir Bhattacharya, Yahiro (Roy) Uemura
  • Patent number: 6281336
    Abstract: The present invention relates to a process for purifying immunoglobulin G from a crude immunoglobulin-containing plasma protein fraction. Said process includes a number of steps of which the anion exchange chromatography and the cation exchange chromatography are preferably connected in series. An acetate buffer having a pH of about 5.0-6.0 and having a molarity of about 5-25 mM is preferably used throughout the purification process. The invention further comprises an immunoglobulin product which is obtainable by this process. The invention also relates to an immunoglobulin product which has a purity of more than 98%, has a content of IgG monomers and dimers of more than 98.5%, has a content of IgA less than 4 mg of IgA/l, and contains less than 0.5% polymers and aggregates. Said product does not comprise detergent, PEG or albumin as a stabilizer. The product is stable, virus-safe, liquid and ready for instant intravenous administration.
    Type: Grant
    Filed: June 9, 1999
    Date of Patent: August 28, 2001
    Assignee: Statens Serum Institut
    Inventors: Inga Laursen, Børge Teisner
  • Patent number: 6239261
    Abstract: A process for the preparation of a concentrate of von Willebrand factor is described, entailing a solution of a complex of this factor with factor VIII:C being optionally pasteurized and treated with an anion exchanger, there being no binding of the von Willebrand factor.
    Type: Grant
    Filed: June 2, 1994
    Date of Patent: May 29, 2001
    Assignee: Aventis Behring GmbH
    Inventors: Norbert Heimburger, Gerhard Kumpe, Klaus Wellner
  • Patent number: 6166187
    Abstract: A sample is prepared from blood in a manner which makes it possible to further analyze proteins in the sample, e.g. to detect prions in the sample. Blood is extracted, allowed to clot and subjected to separation processing (e.g. centrifugation) to obtain serum. The serum is treated with a complexing agent which agent binds prions in the sample forming an agent/protein complex which makes it possible to concentrate the complex. Concentration of the complex results in a sample which can be successfully analyzed, e.g. assayed using a range of different types of assay methodologies for detecting prions.
    Type: Grant
    Filed: March 5, 1999
    Date of Patent: December 26, 2000
    Assignee: The Regents of the University of California
    Inventors: Stanley B. Prusiner, Jiri G. Safar
  • Patent number: 6160092
    Abstract: The present invention provides a crystal of the core portion of the STAT protein in dimeric form with an 18-mer duplex DNA that contains a binding site for the STAT-dimer. The crystal is of sufficient quality to perform X-ray crystallographic studies. Methods of preparing the crystals are include in the invention. The present invention further discloses the three-dimensional structure of the crystal. The present invention also provides methods of using the structural information in drug discovery and drug development.
    Type: Grant
    Filed: May 29, 1998
    Date of Patent: December 12, 2000
    Assignee: The Rockefeller University
    Inventors: Xiaomin Chen, Uwe Vinkemeier, Yanxiang Zhao, David Jeruzalmi, James E. Darnell, Jr., John Kuriyan
  • Patent number: 6140469
    Abstract: The present invention relates to the production of an isoflavone enriched vegetable protein isolate in which the weight ratio of material to extractant is controlled and washing of the acid precipitated protein curd is avoided or minimized to provide an increased level of isoflavones in the protein isolate.
    Type: Grant
    Filed: October 12, 1993
    Date of Patent: October 31, 2000
    Assignee: Protein Technologies International, Inc.
    Inventors: Jerome L Shen, Balagtas Francisco Guevara, Frank Enrico Spadafora
  • Patent number: 6117984
    Abstract: Divalent cation crystals of human growth factor (hGH) or derivatives thereof, and pharmaceutical preparations comprising divalent cation crystals of hGH. In specific embodiments, the divalent cation is Zn++ and the molar ration Zn++ and hGH is about 0.2 to about 10.
    Type: Grant
    Filed: April 21, 1998
    Date of Patent: September 12, 2000
    Assignee: Novo Nordisk A/S
    Inventors: Flemming Junker, Claus Friis Theisen
  • Patent number: 6080844
    Abstract: The present invention involves a process of purifying and recovering a recombinant protein from a suspension of cells. The process of the present invention involves extracting a recombinant protein from a concentrated suspension of cells using a water-miscible organic solvent, isolating the recombinant protein from the suspension of cells, concentrating the recombinant protein to remove the water-miscible organic solvent, precipitating the recombinant protein using an acid, washing the recombinant protein with the salt or free form of a suitable organic acid and recovering the recombinant protein.
    Type: Grant
    Filed: April 23, 1998
    Date of Patent: June 27, 2000
    Assignee: Abbott Laboratories
    Inventors: Ronald E. Carney, Joseph Arndt, Julie R. List, Ellen Marie Schwartz
  • Patent number: 6051694
    Abstract: Liquefied gases, compressed gases, and supercritical fluids are used to form protein particles without first dissolving the protein. The product material is expected to retain full activity and be devoid of residual processing chemicals such as solvents, salts, or surfactants.
    Type: Grant
    Filed: September 17, 1998
    Date of Patent: April 18, 2000
    Inventors: Trevor Percival Castor, Glenn Thomas Hong
  • Patent number: 6051687
    Abstract: A method for removing excess salts, sulfites, sulfates and fatty components from liquid protein hydrolysate is described. Purified liquid protein products having reduced concentrations of salts, sulfites, sulfates and fatty material are also described. The purified liquid protein hydrolysate product can be characterized as having lower than about 0.5% sulfites or sulfates and can also be enhanced with nutrients such as phosphate, potassium, manganese, copper, calcium, magnesium, iron, cobalt, zinc or amino acid such as methionine or lysine.
    Type: Grant
    Filed: February 22, 1999
    Date of Patent: April 18, 2000
    Assignee: Nutra-Flo Company
    Inventors: Doyle Ervin Meeker, Eric James Lohry