Solvent Extraction Patents (Class 530/422)
  • Publication number: 20150024462
    Abstract: The present disclosure relates to improved expanded bed adsorption processes for isolating proteins from milk sources. In particular embodiments, the present disclosure provides a process for isolating a milk protein, such as lactoferrin, from a milk source comprising establishing an expanded bed adsorption column comprising a particulate matrix, applying a milk source to the matrix, and eluting the lactoferrin from the matrix with an elution buffer comprising about 0.3 to about 2.0 M sodium chloride.
    Type: Application
    Filed: July 16, 2013
    Publication date: January 22, 2015
    Inventors: Dattatreya Banavara, John D. Alvey, Joseph Andrew Peters, Juan M. Gonzalez
  • Patent number: 8895708
    Abstract: A protocol for pasteurizing microbial cells is disclosed. The protocol has three stages, a first heating stage, a second plateau stage at which the cells are held at a (maximum and) constant temperature, and a third cooling stage. The heating and cooling stages are rapid, with the temperature of the cells passing through 40 to 80° C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5° C./minute and during cooling is at least ?0.5° C./minute. The plateau maximum temperature is from 70 to 85° C. By plotting the pasteurization protocol on a time (t, minutes) versus temperature (T, ° C.) graph, a trapezium is obtained having an area less than 13,000° C. minute. This results in a smaller energy input and a better quality oil results having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.
    Type: Grant
    Filed: May 9, 2012
    Date of Patent: November 25, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Albert Schaap, Daniel Verkoeijen
  • Patent number: 8821955
    Abstract: Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to the removal of fiber from an oilseed meal using low g-force centrifugation.
    Type: Grant
    Filed: November 10, 2010
    Date of Patent: September 2, 2014
    Assignee: Siebte PMI Verwaltungs GmbH
    Inventor: Qingnong Nelson Tang
  • Patent number: 8815263
    Abstract: The invention provides tandem facial amphiphiles for biochemical manipulations and characterization of membrane proteins, such as intrinsic membrane proteins. Members of this new family display favorable behavior with several membrane proteins. These amphiphiles can form relatively small micelles, and small changes in amphiphile chemical structures can result in large changes in their physical properties. The tandem facial amphiphiles can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: August 26, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Pil Seok Chae, Samuel Helmer Gellman
  • Publication number: 20140228550
    Abstract: A process is provided for obtaining proteins from native substance mixtures, wherein a native substance mixture is first finely disintegrated and optionally processed to form a flowable slurry (I) by adding liquid.
    Type: Application
    Filed: June 6, 2012
    Publication date: August 14, 2014
    Applicant: GEA Mechanical Equipment GmbH
    Inventors: Steffen Hruschka, Wladislawa Boszulak
  • Patent number: 8784926
    Abstract: The present invention relates to methods for the separation of various components from eggs. More particularly, the present invention relates to methods for the separation of proteins and lipids from eggs, including technical eggs (inedible) or edible eggs, yolks or whites, which comprises cross-linking the lipids of eggs with a cross-linking reagent. In an embodiment, the method includes separating the proteins from the cross-linked lipids. In an embodiment, the method includes the separation of various components associated with the cross-linked lipids. The methods disclosed herein allow for the isolation of multiple different components from the egg in an efficient, cost-effective manner without compromising the recovery process of the components or their subsequent utility in various applications or compositions. The compositions and isolated components obtained by the methods of the invention can be used in pharmaceutical, medical, nutritional, cosmetic or health applications.
    Type: Grant
    Filed: November 4, 2011
    Date of Patent: July 22, 2014
    Assignee: Biova, L.L.C.
    Inventors: Ronald E. Strohbehn, Jesse I. Figgins
  • Patent number: 8765923
    Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
    Type: Grant
    Filed: December 20, 2011
    Date of Patent: July 1, 2014
    Assignee: Heliae Development, LLC
    Inventor: Aniket Kale
  • Patent number: 8754168
    Abstract: A method is provided for solubilising a membrane protein. The method is applied to cellular material comprising the membrane protein and an associated membrane lipid. A copolymer of styrene and maleic acid, wherein the styrene:maleic acid ratio is between 1:2 and 10:1, is mixed with the cellular material to cause the copolymer, lipid and protein to form soluble macromolecular assemblies.
    Type: Grant
    Filed: July 8, 2010
    Date of Patent: June 17, 2014
    Assignee: The University of Birmingham
    Inventors: Timothy Dafforn, Michael Overduin, Timothy Knowles
  • Patent number: 8748588
    Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
    Type: Grant
    Filed: October 7, 2011
    Date of Patent: June 10, 2014
    Assignee: Heliae Development, LLC
    Inventor: Aniket Kale
  • Patent number: 8741629
    Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
    Type: Grant
    Filed: November 4, 2011
    Date of Patent: June 3, 2014
    Assignee: Heliae Development, LLC
    Inventor: Aniket Kale
  • Publication number: 20140127779
    Abstract: Polydisperse and charged polysaccharides are fractionated into low polydispersity fractions (preferably having pd<1.1), each containing species within a narrow range of molecular weights. An aqueous solution of the polydisperse polysaccharides is contacted with an ion exchange resin in a column and the polysaccharides are subjected to selective elution by aqueous elution buffer. The selective elution consists of at least 3 sequential elution buffers having different and constant ionic strength and/or pH and in which the subsequent buffers have ionic strength and/or pH than those of the preceding step. The new preparations are particularly suitable for the production of PSA-derivatised therapeutic agents intended for use in humans and animals.
    Type: Application
    Filed: January 15, 2014
    Publication date: May 8, 2014
    Applicant: Lipoxen Technologies Limited
    Inventors: Sanjay Jain, Ioannis Papaioannou, Peter Laing
  • Patent number: 8658772
    Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
    Type: Grant
    Filed: October 13, 2011
    Date of Patent: February 25, 2014
    Assignee: Heliae Development, LLC
    Inventor: Aniket Kale
  • Publication number: 20140024040
    Abstract: The disclosure relates generally to methods, systems, compositions and kits for breaking a water-in-oil emulsion including one or more biomolecules dispersed in an aqueous phase of the water-in-oil emulsion. In some embodiments, the disclosure relates to obtaining a first emulsion including a continuous hydrophobic fraction and a discontinuous aqueous fraction, the aqueous fraction having one or more biomolecules dispersed therein, breaking the first emulsion by contacting the first emulsion with a breaking solution including a second emulsion, where the second emulsion includes a discontinuous phase of organic extraction solvent dispersed in a continuous aqueous phase, and centrifuging to separate the phases of the resulting mixture.
    Type: Application
    Filed: April 5, 2012
    Publication date: January 23, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jeffrey Sabina, Ilya Zlatkovsky, Rachel Kasinskas
  • Patent number: 8623445
    Abstract: Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to the removal of fiber from an oilseed meal using low g-force centrifugation.
    Type: Grant
    Filed: November 11, 2009
    Date of Patent: January 7, 2014
    Assignee: Bio-Extraction Inc.
    Inventor: Qingnong Nelson Tang
  • Patent number: 8574587
    Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
    Type: Grant
    Filed: November 4, 2011
    Date of Patent: November 5, 2013
    Assignee: Heliae Development, LLC
    Inventor: Aniket Kale
  • Patent number: 8557963
    Abstract: A process of aqueous protein extraction from Brassicaceae oilseed meal, such as canola, commercial canola meal or yellow mustard, to obtain a napin-rich protein extract, a cruciferin-rich protein extract, and a low-protein residue. The process comprising the steps of performing aqueous extraction of the Brassicaceae oilseed meal at a pH of from about 2.5 to about 5.0 to obtain a soluble napin-rich protein extract and a cruciferin-rich residue followed by performing aqueous extraction of the cruciferin-rich residue to obtain a soluble cruciferin-rich protein extract and a low-protein residue. The cruciferin-rich residue may be treated with cell wall degrading enzymes to obtain a cruciferin-rich fraction The cruciferin-rich protein products may be substantially free of napin protein and may be useful as a non-allergenic food product for human consumption.
    Type: Grant
    Filed: May 30, 2008
    Date of Patent: October 15, 2013
    Assignee: Her Majesty the Queen in Right of Canada, as Represented by the Minister of Agriculture and Agri-food
    Inventors: Janitha P. D. Wanasundara, Tara C. McIntosh
  • Publication number: 20130266656
    Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
    Type: Application
    Filed: March 14, 2013
    Publication date: October 10, 2013
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen
  • Patent number: 8552160
    Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
    Type: Grant
    Filed: April 6, 2011
    Date of Patent: October 8, 2013
    Assignee: Heliae Development, LLC
    Inventor: Aniket Kale
  • Patent number: 8529981
    Abstract: Protein concentrates and protein isolates, in addition to processes for the production of protein concentrates and protein isolates, are disclosed. In particular, the disclosure relates to a process for removing fiber from an oilseed meal, comprising: i) mixing an oilseed meal with a blending solvent, optionally water, saline solution, polysaccharide solution or protein containing solution, to form a mixture; ii) optionally adjusting the pH of the protein slurry to a pH of about 2 to about 10; and iii) separating the mixture to form a protein slurry comprising soluble and insoluble proteins and an insoluble fiber fraction.
    Type: Grant
    Filed: May 15, 2009
    Date of Patent: September 10, 2013
    Assignee: Bioexx Specialty Proteins Ltd.
    Inventor: Qingnong Nelson Tang
  • Patent number: 8476412
    Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
    Type: Grant
    Filed: April 6, 2011
    Date of Patent: July 2, 2013
    Assignee: Heliae Development, LLC
    Inventor: Aniket Kale
  • Patent number: 8466265
    Abstract: A method of preparing anti-b amyloid immunoglobulin involves treating human plasma anti-b amyloid immunoglobulin under alkaline conditions, such as at pH 10.25 to 11.75 using diethylamine HCl, to dissociate b amyloid protein therefrom. Typically, the anti-b amyloid immunoglobulin is present in human immunoglobulin preparations obtained from plasma or serum. Anti-b amyloid immunoglobulin prepared by the method is substantially free of b amyloid protein and has therapeutic activity in compositions and/or methods for treating a disease or condition associated with b amyloid plaques, such as Alzheimer's disease.
    Type: Grant
    Filed: October 2, 2008
    Date of Patent: June 18, 2013
    Assignee: CSL Limited
    Inventor: John Cox
  • Publication number: 20130115251
    Abstract: The invention provides tandem facial amphiphiles for biochemical manipulations and characterization of membrane proteins, such as intrinsic membrane proteins. Members of this new family display favorable behavior with several membrane proteins. These amphiphiles can form relatively small micelles, and small changes in amphiphile chemical structures can result in large changes in their physical properties. The tandem facial amphiphiles can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.
    Type: Application
    Filed: November 5, 2012
    Publication date: May 9, 2013
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventor: Wisconsin Alumni Research Foundation
  • Patent number: 8404644
    Abstract: The invention relates to the use of angiogenic crystallin proteins to promote angiogenesis, wound healing and/or endothelial cell migration. Alpha A crystallin and ?B2 crystallin have particular application in these methods. The crystallins will usually be in monomeric form. Typically, truncated form(s) of ?B2 crystallin protein are utilized as can be prepared by partial hydrolysis of the protein by a protease enzyme such as elastase I. Methods for the purification of crystallin proteins from eye tissue are also described.
    Type: Grant
    Filed: September 5, 2008
    Date of Patent: March 26, 2013
    Assignees: Meat & Livestock Australia Limited, Industrial Research Limited
    Inventors: Keryn Johnson, Madhusudan Vasudevamurthy
  • Patent number: 8399633
    Abstract: During the production of recombinant proteins from gram negative bacteria, lipopolysaccharides (LPS, endotoxin) are released along with the protein of interest. In many instances, LPS will copurify with the target protein due to specific or non-specific protein-ILPS interactions. We have investigated the ability of alkanediols to effect the separation of LPS from protein-LPS complexes while the complexes are immobilized on anion or cation exchange chromatographic media. Alkanediols provide a safer alternative to the use of other organics such as alcohols or acetonitrile due to their lower toxicity and decreased flammability. In addition, they are less costly than many of the detergents that have been used for such purposes. LPS removal efficiency increased with increasing alkane chain length. 1,2-alkanediols were more effective than terminal alkanediols in the separation of LPS from protein LPS complexes.
    Type: Grant
    Filed: May 19, 2010
    Date of Patent: March 19, 2013
    Assignee: Fujifilm Diosynth Biotechnologies U.S.A., Inc.
    Inventors: Philip Alfred Ropp, Michael Van Alen Murray
  • Patent number: 8389687
    Abstract: Blood collection, processing and transfer leads to the separation of discrete fractions by adding additional citrate (trisodium citrate) to bring the citrate concentration to 10%-15% w/v thereby leading to enhanced yield and purity of cryoprecipitate. The improved cryoprecipitate then yields concentrated clotting factors by an improved extraction process which uses polyvinyl pyrollidone to reduce the extraction of fibrinogen. Following extraction the remaining cryoprecipitate can advantageously be formed into a fibrin fabric used in surgeries and in the treatment of wounds.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: March 5, 2013
    Assignee: Shanbrom Technologies, LLC
    Inventor: Edward Shanbrom
  • Patent number: 8344107
    Abstract: Methods and apparatus for processing corn into one or more corn products. Oil is extracted from corn or corn products or by-products with a solvent. The corn-solvent mixture is separated into streams, one of which preferably includes an extract containing at least oil and solvent, and another containing de-oiled corn solids and adsorbed solvent. Zein is separated from the de-oiled corn solids. Solvent is then separated, and the de-oiled, de-zeined, desolventized corn solids are processed to provide one or more corn products.
    Type: Grant
    Filed: July 14, 2009
    Date of Patent: January 1, 2013
    Assignee: The Board of Trustees of the University of Illinois
    Inventor: Munir Cheryan
  • Patent number: 8344108
    Abstract: Methods and apparatus for processing corn into one or more corn products. Zein is extracted from corn or corn products or by-products with a solvent. The corn-solvent mixture is separated into streams, one of which preferably includes an extract containing at least zein and solvent, and another that contains de-zeined corn solids and adsorbed solvent. The solvent is separated from the zein, and the de-zeined, desolventized corn solids are processed to provide one or more corn products.
    Type: Grant
    Filed: July 21, 2009
    Date of Patent: January 1, 2013
    Assignee: The Board of Trustees of the University of Illinois
    Inventor: Munir Cheryan
  • Publication number: 20120251614
    Abstract: In one aspect, the invention provides methods for manufacturing, processing and recovering organic matter from a biomass, e.g., a biofluid or biosemisolid comprising a protein and a lipid, for example, from a dairy-based material. In one aspect, the invention provides methods for encapsulating oils with proteins for, e.g., making foods, food supplements or additives and feeds or feed supplements or additives. Compositions made by processes of this invention can be used for foods, food supplements or additives, feeds or feed supplements or additives, and/or biofuels.
    Type: Application
    Filed: November 1, 2011
    Publication date: October 4, 2012
    Applicant: Parma Laboratories, Inc
    Inventor: Gary ROHWER
  • Publication number: 20120184720
    Abstract: The present disclosure includes a process for separating protein and an organic solvent from a defatted biomass comprising: a) contacting the defatted biomass with an aqueous solvent to: i) extract protein from the defatted biomass into the aqueous solvent and form an aqueous protein solution; and ii) reduce the concentration of the organic solvent in the defatted biomass and form an organic solvent fraction; b) separating the organic solvent fraction from the aqueous protein solution, wherein the organic solvent is present in the defatted biomass prior to the contacting step at a concentration of at least 100 ppm.
    Type: Application
    Filed: July 2, 2010
    Publication date: July 19, 2012
    Inventors: Luis Rozenszain, Jocelyn Doucet
  • Patent number: 8217151
    Abstract: An improved pasteurisation protocol for pasteurising microbial cells is disclosed. The protocol has three stages, a first heating stage, a second plateau stage at which the cells are held at a (maximum and) constant temperature, and a third cooling stage. Both the heating and the cooling stages are rapid, with the temperature of the cells passing through 40 to 80° C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5° C./minute and during cooling is at least ?0.5° C./minute. The plateau maximum temperature is from 70 to 85° C. By plotting the pasteurisation protocol on a time (t, minutes) versus temperature (T, ° C.) graph, one obtains a trapezium having an area less than 13,000° C. minute. Not only does this result in a smaller energy input (and so a reduction in costs), but a better quality (and less oxidised) oil results having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.
    Type: Grant
    Filed: March 9, 2009
    Date of Patent: July 10, 2012
    Assignee: DSM IP Assets B.V.
    Inventors: Albert Schaap, Daniel Verkoeijen
  • Publication number: 20120130054
    Abstract: The methods disclosed herein are useful for achieving higher protein concentrations in reverse micelle solutions by extracting a protein from a metal chelate resin using a reverse micelle solution comprising a polyamine that competitively binds to the metal chelate resin, allowing the protein to elute from the resin. The extracted protein in the reverse micelle solution can then be effectively analyzed, for example using NMR spectroscopy.
    Type: Application
    Filed: November 19, 2010
    Publication date: May 24, 2012
    Inventor: Ronald W. Peterson
  • Publication number: 20120083426
    Abstract: A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.
    Type: Application
    Filed: July 21, 2011
    Publication date: April 5, 2012
    Inventors: Claus Lindvald JOHANSEN, Soren Kjaerulff, Susan Mampusta Madrid, Henrik Pedersen, Charlotte Horsmans Poulsen, Masoud Rajabi Zargahi
  • Patent number: 8143381
    Abstract: The present invention relates to the use of ionic liquids or of mixtures comprising at least one ionic liquid and at least one further solvent for the extraction of membrane proteins from biological samples, to methods for the extraction of membrane proteins, to a kit for the extraction of these proteins and to the use thereof.
    Type: Grant
    Filed: October 18, 2007
    Date of Patent: March 27, 2012
    Assignee: Merck Patent GmbH
    Inventors: Joerg Von Hagen, Uwe Michelsen, Maria Wehsling
  • Publication number: 20120071414
    Abstract: The present invention relates to a method for extracting organic molecules and/or organic minerals from a limestone material, including placing said limestone material in contact with a solution including at least one organic solvent, water and at least one acid, as well as to the organic molecules and/or organic minerals produced by the method. The products produced by the method can be used, for example, in therapeutic and non-therapeutic fields, in particular the agri-food, cosmetic and medical fields.
    Type: Application
    Filed: April 9, 2010
    Publication date: March 22, 2012
    Applicants: Centre National De La Recherche Scientifique-CNRS-, Universite D'Orleans, Museum National D'Histoire Naturelle
    Inventors: Xavier Bourrat, Evelyne Lopez, Marthe Rousseau
  • Publication number: 20120065382
    Abstract: The present invention is one that, in a pollen protein extracting method, enables mass production, and while improving production efficiency, achieves a reduction in workload such as a reduction in man-hours, reductions in equipment cost and running cost, and an improvement of a work environment. Specifically, a pollen protein extracting method for extracting water-soluble protein from pollen is characterized by including: mixing the pollen and PBS; adding an aggregating agent including a natural inorganic component to a resultant mixed solution and performing stirring; and after formation of a pollen aggregate, performing filter filtration to perform solid-liquid separation.
    Type: Application
    Filed: May 10, 2010
    Publication date: March 15, 2012
    Applicants: PROTEC CO., LTD., WAKO FILTER TECHNOLOGY CO., LTD.
    Inventors: Tsuneo Yamaguchi, Akira Saito, Akio Kato
  • Patent number: 8133521
    Abstract: The present invention relates to methods for the separation of various components from eggs. More particularly, the present invention relates to methods for the separation of proteins and lipids from eggs, including technical eggs (inedible) or edible eggs, yolks or whites, which comprises cross-linking the lipids of eggs with a cross-linking reagent. In an embodiment, the method includes separating the proteins from the cross-linked lipids. In an embodiment, the method includes the separation of various components associated with the cross-linked lipids. The methods disclosed herein allow for the isolation of multiple different components from the egg in an efficient, cost-effective manner without compromising the recovery process of the components or their subsequent utility in various applications or compositions. The compositions and isolated components obtained by the methods of the invention can be used in pharmaceutical, medical, nutritional, cosmetic or health applications.
    Type: Grant
    Filed: January 9, 2008
    Date of Patent: March 13, 2012
    Assignee: Biova, L.L.C.
    Inventors: Ronald E. Strohbehn, Jesse I. Figgins
  • Publication number: 20120053327
    Abstract: A method for separating proteins from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting proteins from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal proteins from a wet algal biomass. These proteins are high value products which can be used as renewable sources of food and food additives. Neutral lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
    Type: Application
    Filed: November 1, 2011
    Publication date: March 1, 2012
    Applicant: Heliae Development, LLC
    Inventor: Aniket Kale
  • Publication number: 20120052552
    Abstract: The present invention relates to methods for the separation of various components from eggs. More particularly, the present invention relates to methods for the separation of proteins and lipids from eggs, including technical eggs (inedible) or edible eggs, yolks or whites, which comprises cross-linking the lipids of eggs with a cross-linking reagent. In an embodiment, the method includes separating the proteins from the cross-linked lipids. In an embodiment, the method includes the separation of various components associated with the cross-linked lipids. The methods disclosed herein allow for the isolation of multiple different components from the egg in an efficient, cost-effective manner without compromising the recovery process of the components or their subsequent utility in various applications or compositions. The compositions and isolated components obtained by the methods of the invention can be used in pharmaceutical, medical, nutritional, cosmetic or health applications.
    Type: Application
    Filed: November 4, 2011
    Publication date: March 1, 2012
    Applicant: BIOVA, L.L.C.
    Inventors: Ronald E. Strohbehn, Jesse I. Figgins
  • Publication number: 20120009287
    Abstract: The present invention relates to a method of obtaining protein preparations from sunflower seeds as well as protein preparations produced with the method. In the method the sunflower seeds are dehulled to a residual hull content of 5% by weight or dehulled sunflower seeds with a residual hull content of 5% by weight are provided. Mechanical partial deoiling of the dehulled sunflower seeds is carried out through pressing up to a fat or oil content of the dehulled sunflower seeds in the range 10 to 35% by weight. After carrying out one or more extraction steps with at least one solvent, a defatted flour containing protein is obtained as a protein preparation. Both optically and functionally the protein preparation has very advantageous properties which allow it to be used directly in the food product or animal feed sector.
    Type: Application
    Filed: February 26, 2010
    Publication date: January 12, 2012
    Inventors: Claudia Pickardt, Peter Eisner, Stephanie Bader, Florian Wild, Klaus Muller
  • Patent number: 8088900
    Abstract: Provided are new methods for precipitating proteins comprising (a) providing a protein solution, (b) adding a salt to the solution, (c) either (i) adjusting pH of the composition to below the pI of the protein (in cases where the method is directed towards precipitation of most or all proteins in the solution) or (ii) adjusting the pH of the composition to above the pI of the protein (in cases where keeping the target protein in solution is desired), and (d) adding an organic compound to the solution, wherein (I) in the case where the method comprises step (c)(i) a two phase solution is formed wherein at least about 75% of the protein is contained in the protein phase or (II) in the case where the method comprises step (c)(ii) the method further comprises removing precipitated impurities from the protein solution.
    Type: Grant
    Filed: March 28, 2007
    Date of Patent: January 3, 2012
    Assignee: Novo Nordisk A/S
    Inventors: Thorkild Christensen, Jan Anton Enoksen
  • Patent number: 8080272
    Abstract: The present invention relates to methods for the separation of various components from eggs. More particularly, the present invention relates to methods for the separation of proteins and lipids from eggs, including technical eggs (inedible) or edible eggs, yolks or whites, which comprises cross-linking the lipids of eggs with a cross-linking reagent. In an embodiment, the method includes separating the proteins from the cross-linked lipids. In an embodiment, the method includes the separation of various components associated with the cross-linked lipids. The methods disclosed herein allow for the isolation of multiple different components from the egg in an efficient, cost-effective manner without compromising the recovery process of the components or their subsequent utility in various applications or compositions. The compositions and isolated components obtained by the methods of the invention can be used in pharmaceutical, medical, nutritional, cosmetic or health applications.
    Type: Grant
    Filed: January 9, 2008
    Date of Patent: December 20, 2011
    Assignee: Biova, L.L.C.
    Inventors: Ronald E. Strohbehn, Jesse I. Figgins
  • Publication number: 20110250639
    Abstract: Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.
    Type: Application
    Filed: September 24, 2010
    Publication date: October 13, 2011
    Inventors: JAMES A. ERNST, DANIEL YANSURA, HOK SEON KIM
  • Publication number: 20110237778
    Abstract: A process for the extraction of macromolecules from a biomass material comprising: a) contacting the biomass material with a solution comprising thin stillage to provide a slurry comprising undissolved solids, dissolved solids and suspended solids; and b) separating undissolved solids from the slurry to provide a solid fraction and a liquid fraction; and wherein the macromolecules are comprised in the dissolved solids
    Type: Application
    Filed: March 28, 2011
    Publication date: September 29, 2011
    Inventors: Martin J. Reaney, Kornsulee Ratanapariyanuch
  • Patent number: 8007718
    Abstract: A method of processing bone material using supercritical fluids is disclosed. The method comprises placing the bone material in a processing chamber, adding supercritical fluid to the processing chamber, pulsing the supercritical fluid in the processing chamber, and rinsing the bone material. A processing system for processing bone material using supercritical fluids in accordance with the present invention comprises a processing chamber for housing the bone material, a vat for storing a processing fluid, a pump, a heating element, a flow path, a tank, and a solvent port.
    Type: Grant
    Filed: May 8, 2008
    Date of Patent: August 30, 2011
    Assignee: SDCmaterials, Inc.
    Inventor: Maximilian A. Biberger
  • Publication number: 20110192073
    Abstract: Methods for selective extraction and fractionation of algal lipids and algal products are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which enable efficient separation of algal components. Among these components are neutral lipids synthesized by algae, which are extracted by the methods disclosed herein for the production of renewable fuels.
    Type: Application
    Filed: April 6, 2011
    Publication date: August 11, 2011
    Applicant: HELIAE DEVELOPMENT, LLC
    Inventor: Aniket KALE
  • Publication number: 20110177582
    Abstract: Embodiments of the process for isolating proteins from a defatted meal comprise extracting the defatted meal with an acidic solvent at a pH of between 2 and 6 to obtain a mixture of treated meal and a first solution of proteins. The process further comprises extracting the treated meal with an alkaline solvent at a pH of between 9 and 12.5 to obtain a mixture of meal residue and a second solution of proteins. The meal residue is separated from the second solution of proteins, and the proteins are separated from the second solution of proteins to obtain one or more protein products.
    Type: Application
    Filed: June 9, 2009
    Publication date: July 21, 2011
    Inventors: Olev Trass, Eduard Tearo, Toivo Trass
  • Publication number: 20110130472
    Abstract: The present invention generally relates to compositions comprising the proteose peptone fraction (PPf). In particular, the present invention relates to a method for the production of an extract comprising a demineralised protein fraction depleted in ?-lactoglobulin and enriched in the PPf and to uses of these extracts, e.g. in a food product, a food supplement, a nutritional, a pharmaceutical and/or a cosmetic composition, for example as emulsifier or as foaming agent. The PPf fraction of the present invention may be obtained by adjustment of the pH of an aqueous native protein dispersion to about 5.6 to 8.4, or to about 3.5 to 5.0, heating the aqueous native protein dispersion to about 70-95° C. for about 10 seconds to 60 minutes, removing at least a part of the formed solid large molecular weight aggregates with a diameter of at least 100 nm from the aqueous protein dispersion after heating and collecting the remaining liquid fraction of the dispersion.
    Type: Application
    Filed: February 18, 2009
    Publication date: June 2, 2011
    Applicant: NESTEC S.A.
    Inventors: Magali Faure, Lionel Jean Rene Bovetto, Philippe Montavon, Christophe Schmitt
  • Publication number: 20110124034
    Abstract: The subject invention relates to novel methods for treating microbial biomass and uses thereof. In particular, this invention provides methods for production of lipids using ionic liquid solutions, and subsequent uses of biomass components in food, biofuels, and as chemical precursors. Further, this invention provides methods for recovering the ionic liquids using an antisolvent, thus enables subsequent reuse of the ionic liquids In addition, this invention provides practical methods for determining the lipid content of a biomass and screening potential lipid-producing microbial classes. This invention also provides a method of chemical dewatering the lipid-rich algae cells by ionic liquids with its subsequent reuse.
    Type: Application
    Filed: November 24, 2010
    Publication date: May 26, 2011
    Inventors: ADELHEID R. KUEHNLE, Norie Anne B. Nolasco
  • Patent number: 7935798
    Abstract: A method for extracting an intracellular peptide, protein or other polypeptide from a whole fermentation broth using a water miscible alcohol, or a water miscible or partially water miscible glycol ether.
    Type: Grant
    Filed: February 18, 2005
    Date of Patent: May 3, 2011
    Assignee: Dow Global Technologies LLC
    Inventors: Timothy C. Frank, Felipe A. Donate, Jefry E. Shields, Kai Li, Jeffrey R. Allen
  • Patent number: 7906150
    Abstract: Shark cartilage extract has been shown to be an antagonist of parathyroid hypertensive factor (PHF). In view of this, shark cartilage extract can be used to treat conditions related to excessive PHF activity. Such diseases include hypertension and some other diseases related to intracellular calcium elevation. Methods for producing the shark cartilage extract and methods for administering the extract are disclosed.
    Type: Grant
    Filed: May 29, 2007
    Date of Patent: March 15, 2011
    Assignee: FX Life Sciences AG
    Inventors: Peter K. T. Pang, Jacqueline J. Shan, Kam Chiu