Abstract: A liquid phase containing preS2+S antigen is separated into two phases after which the desired antigen is concentrated, washed and adsorbed and desorbed from a fumed silica to produce a product that is purified and concentrated in antigen:protein ratio and that is suitable for final purification.
Abstract: A method for the separation or purification of biopolymers comprises adsorbing the biopolymer on the surface of liquid oil droplets; and separating the adsorbed biopolymer from the oil droplets. The adsorbed biopolymer is separated from the oil droplets by mixing the droplets in an aqueous liquid, removing the lower aqueous phase and adding a fresh aqueous phase to the droplets, cooling the mixture to solidify and coalesce the oil and to cause it to release the adsorbed biopolymer to the fresh liquid, separating the fresh liquid and the biopolymer from the coalesced oil, separating the biopolymer from the fresh liquid.
Abstract: A novel process for the preparation of water-soluble acyl glycoprotein extracted from Klebsiella Pneumoniae containing 30 to 45% by weight of proteins, 30 to 40% by weight of neutral saccharides, less than 4% of glucuronic acid, 2 to 5% by weight of osamines with a molecular weight of about 350,000 daltons and having a polysaccharide chain of n chains of one molecule of glucose and 4 molecules of galactose attached to an asparagine of a proteidic chain by a core formed of heptose and 2-keto-3-deoxy-octulosonic acid followed by an acyl portion containing .beta.-hydroxymyristic acid and then N-acetyl-glucosamine designated herein as F.sub.1 and compositions and a method of inducing antiallergic properties in warm-blooded animals.
Abstract: A method for the solubilization and recovery of insoluble parasite protective antigenic factors associated with parasite material comprising solubilizing the antigenic factors with a non-ionic detergent and separating the solubilized material from undispersed residual material. The purified protective antigenic factors are useful as vaccines, particularly against malaria, and as diagnostic agents.
Abstract: HSA crystals are provided in the form of tetragonal plates having the space groups P42.sub.1 2, the crystals being grown to sizes in excess of 0.5 mm in two dimensions and a thickness of 0.1 mm. Growth of the crystals is carried out by a hanging drop method wherein a precipitant solution containing PEG and a phosphate buffer is mixed with an HSA solution, and a droplet of mixed solution is suspended over a well of precipitant solution. Crystals grow to the desired size in 3 to 7 days. Concentration of reagents, pH and other parameters are controlled within prescribed limits. The resulting crystals exhibit a size and quality such as to allow performance of x-ray diffraction studies and enable the conduct of drug binding studies as well as genetic engineering studies.
Type:
Grant
Filed:
August 20, 1987
Date of Patent:
May 23, 1989
Assignee:
The United States of America as represented by the Administrator of the National Aeronautics and Space Administration
Abstract: An improved vector upon introduction into a suitable bacterial host containing the thermolabile repressor C.sub.I renders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of a desired gene inserted into the vector and production of polypeptide encoded by the gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: a DNA sequence which contains the promoter and operator P.sub.L O.sub.
Abstract: A novel leaching process for separating extractable material from a particulate solid material that comprises extractable and non-extractable portions includes the steps of introducing the particulate solid material and a liquid solvent to an extraction zone; agitating the liquid solvent to a degree sufficient to suspend the particulate solid material and effect segregation of particles thereof in relation to their propensity to settle; maintaining contact between the liquid solvent and the particulate solid material for a time sufficient to leach extractable material therefrom; and selectively withdrawing particles of the solid material from the extraction zone. In a preferred embodiment, the leaching process is a multi-stage process in which the selectively withdrawn particles from each stage are introduced to the next successive stage of the series.
Abstract: Guanidine hydrochloride (GCl) is used to remove excess sodium dodecyl sulfate (SDS) from SDS-solubilized protein solutions, and particularly from SDS-solubilized inclusion body solutions, GCl is added to the solution containing SDS to induce the formation of a GCl-SDS complex (GDS) which, when allowed to precipitate, can easily be removed by centrifugation, filtration, or other suitable means.
Abstract: A process is provided for purifying recombinant IL-1 from microbial cells, comprising suspending the cells in an aqueous buffered medium having a pH from about 1 to about 5; disrupting the cells to provide an extract containing solubilized IL-1; and recovering solubilized Il-1 from the extract.
Abstract: A method for the recovery of proteins which method includes providing a source of protein in an insoluble form, a source of at least one cationic surfactant; treating the insoluble protein with the at least one cationic surfactant in an amount sufficient to effect solubilization without substantial modification to the structural backbone of the protein.
Abstract: Basic Fibroblast Growth Factor (FGF) is substantially purified by the employment of affinity chromatography using heparin-linked support material. Described is a simplified three step procedure for extracting basic FGF from either mammalian brain or mammalian pituitary tissue. Salt precipitation, e.g., with ammonium sulfate is used to provide a partially purified precipitate that is then subjected to ion-exchange chromatography, e.g., using a Carboxymethyl-Sephadex column. Substantially pure basic FGF fractions are then obtained by fractionating the further partially purified fractions using affinity chromatography on a heparin-linked support e.g., Heparin-Sepharose.
Type:
Grant
Filed:
December 10, 1986
Date of Patent:
November 15, 1988
Assignee:
The Salk Institute for Biological Studies
Inventors:
Denis Gospodarowicz, Andrew Baird, Peter Bohlen
Abstract: Purification and solubilization of proteins produced in transformant microorganisms as insoluble, biologically inactive inclusion bodies is effected by solubilizing the inclusion bodies in SDS; treating the SDS-protein solution with urea; removing the SDS and purifying the protein by chromatography on an anion-exchange resin having cationic groups attached to a polysaccharide support; and dialyzing the solution obtained from the anion-exchange resin to remove urea, thereby allowing the protein to fold into its native conformation. The solution thus obtained can be activated by removing soluble protein aggregates via ultrafiltration or chromatography on a weak anion-exchange column.
Abstract: A refractile material containing a heterologous protein is recovered from a host microorganism cell culture transformed to produce the protein. One recovery process involves disrupting the cell wall and membrane of the host cell, removing greater than 99% by weight of the salts from the disruptate, redisrupting the desalted disruptate, adding a material to the disruptate to create a density or viscosity gradient in the liquid within the disruptate, and separating the refractile material from the cellular debris by high-speed centrifugation. Another version of such a recovery process comprises the further steps of solubilizing the refractile material under reducing conditions, organically extracting the solubilized refractile material, and isolating said refractile material from the extractant.Preferably the protein is recombinant IL-2 or IFN-.beta. and the salt removal step is carried out by diafiltration.
Type:
Grant
Filed:
March 25, 1986
Date of Patent:
May 31, 1988
Assignee:
Cetus Corporation
Inventors:
Glenn Dorin, Wolfgang H. Hanisch, Leo S. Lin
Abstract: Process for preparing a detoxified polysaccharide-outer membrane protein complex from bacterial envelopes; the so-obtained products which are useful as vaccines against infection by the same bacteria and method for protecting animals against the same infection by administration of a pharmaceutical composition containing the detoxified polysaccharide-outer membrane protein complexes.
Type:
Grant
Filed:
September 17, 1985
Date of Patent:
November 17, 1987
Assignee:
The United States of America as represented by the Secretary of the Army
Inventors:
Wendell D. Zollinger, John Boslego, Ellen Moran, Brenda Brandt, Hugh Collins, Robert Mandrell, Patricia Altieri, Sanford Berman
Abstract: A method is disclosed for the recovery of biological material from an aqueous solution comprising contacting a water-insoluble, particulate binder with a solution containing biological material to produce a water insoluble biological material/binder composition which may then be recovered. The aqueous solutions may then be recycled.
Type:
Grant
Filed:
February 12, 1986
Date of Patent:
September 1, 1987
Assignee:
Union Carbide Corporation
Inventors:
Kavssery P. Ananthapadmanabhan, Errol D. Goodard
Abstract: Purification and activation of proteins produced in transformant microorganisms as insoluble, biologically inactive inclusion bodies can be effected by solubilizing the inclusion bodies in SDS; dialyzing or refrigerating the protein solution to remove a portion of the SDS; chromatographing the protein solution on an ion-retardation resin to remove the remaining SDS from the protein; and chromatographing the protein solution thus obtained on an anion-exchange resin.
Abstract: A method for extracting protein produced by procaryotic or eucaryotic cells comprising contacting said cells with a solution containing from about 50 to about 100 volume percent of an organic acid having from 1 to 5 carbon atoms and mixtures thereof is disclosed.
Abstract: A method is provided for forming ordered macromolecular protein arrays by avoiding a binding pathway that leads to densely packed, disordered states during protein crystal growth, by a diffusion limited process or by allowing controlled growth to occur by removal of the initial supported protein layer to a different environment.
Abstract: Crude HCG is purified by extracting with a neutral or weakly basic aqueous solution containing lower aliphatic alcohol and soluble salt, adding lower aliphatic alcohol to the extracted solution to form precipitates and the precipitates containing high purity of HCG are collected. This precipitates can be further purified by dissolving in a buffer solution, contacting the solution with a weak anion exchanger and eluting the exchanger with said buffer solution containing added salt.
Abstract: An improved process is described for recovering proteins from insoluble inclusion bodies produced in transformant microorganisms. Sidestream precipitates isolated from chromatography effluent are resolubilized in a denaturing agent and the resolubilized proteins are renatured either by direct dialysis into denaturant-free buffer or by dialysis into a weaker denaturing agent followed by dialysis into denaturant-free buffer.