Abstract: Chemically synthesized polypeptides include amino acid residue sequences that substantially correspond to the amino acid residue sequences of T cell and B cell determinant portions of a natural, pathogen-related protein, in particular, a hepatitis B virus surface antigen (HBsAg). When administered to a host alone, as polymers or as carrier-bound conjugates, the polypeptides induce the proliferation of thymus-derived cells in hosts primed against hepatitis B virus.

Abstract: Chemically synthesized polypeptides include amino acid residue sequences that substantially correspond to the amino acid residue sequences of T Cell and B cell determinant portions of a natural, pathogen-related protein, in particular, a hepatitis B virus surface antigen (HBsAG). When administered to a host alone, as polymers or as carrier-bound conjugates, the polypeptides induce the proliferation of thymus-derived cells in hosts primed against hepatitis B virus.

Abstract: Peptide fragments of streptococcal M protein named CB3, CB4, and CB5 and related synthetic peptides have been identified. When linked to a protein carrier which is polylysine the conjugates of CB3, CB4, CB5, and S-CB3 are capable of evoking type specific opsonic antibodies against streptococci.

Abstract: Several novel hybridoma cell lines, ATCC #HB-8510, 8511, 8512, 8513, 8514, 8515, 8516, and 8517 produce monoclonal antibody to an antigen, peptidoglycan, which is a normal structural component of neREFERENCE TO GOVERNMENTThe invention described herein was supported by National Institutes of Health grants DE-03487 and DE-05160.

Abstract: Novel water-soluble, immunostimulating glycoproteins extracted from Klebsiella pneumoniae containing 30 to 45% by weight of proteins, 30 to 40% by weight of neutral saccharides, up to 4% by weight of glucuronic acid, 2 to 5% by weight of osamines and having a molecular weight of about 350,000 daltons.

Abstract: A method of preparing simultaneously monoclonal antibodies specific for different cell-free products of activated human T lymphocytes is disclosed. Human T cells are activated in a medium supplemented with mouse serum rather than conventional calf serum. A supernatant prepared from the activated T cells is used to immunize mice. The dominant immunogens in the supernatant are the cell-free products of human T lymphocytes. The yield of hybrid cells which produce products reactive with cell-free products of human T lymphocytes is enhanced by injecting the immunized mice with a supernatant from mitogen-activated murine splenocytes. In addition, a novel radioimmunoadsorbent assay for screening hybrids to detect production of monoclonal antibodies reactive with cell-free products of human T lymphocytes is disclosed.

Abstract: This invention is directed to AUT-PK 500, a novel autophosphorylating protein kinase, to the purification and characterization of AUT-PK 500 from rat adrenocortical carcinoma, to the use of AUT-PK 500 as a marker for neoplasia cells, and to a radioimmunoassay for detecting AUT-PK 500 in neoplasia cells.

Abstract: An antigenic material having a molecular weight of 3800-4500, and useful in the preparation of an anticaries vaccine is produced from a known antigenic material, antigen I/II of molecular weight 185,000 daltons obtained from the culture supernatant of Streptococcus mutans. The smaller molecular weight fraction of the invention contains the same antigenic determinants as the known 185,000 dalton material but the lower molecular weight material can be used with less side effects. The antigenic material can be used for the production of vaccine preparations that could be administered parenterally or by topical application to the gums.

Abstract: The protein PP.sub.20 is described, this having the following characteristics:(a) an electrophoretic mobility which is somewhat larger than that of albumin,(b) an isoelectric point of 4.65.+-.0.1,(c) a sedimentation coefficient s.sub.20,w of 4.1.+-.0.1 S,(d) a molecular weight determined by electrophoresis in sodium dodecyl sulfate (SDS)-containing polyacrylamide gel of 50,000.+-.10,000, the molecules of PP.sub.20 being composed of apparently identical subunits which have a molecular weight of 27,000.+-.3,000 and are held together non-covalently,(e) an extinction coefficient E.sub.1cm.sup.1% (280 nm) of 9.5.+-.0.6,(f) a carbohydrate content of 3.0.+-.1.3%, including mannose 0.14.+-.0.05%, fucose 0.13.+-.0.05%, galactose 0.61.+-.0.2%, glucose 0.39.+-.0.2%, N-acetylglucosamine 0.95.+-.0.3%, N-acetylgalactosamine 0.18.+-.0.1%, and N-acetylneuraminic acid 0.6.+-.0.4%.(g) a specified aminoacid composition, and a process for obtaining it is described.

Abstract: The present invention concerns an autologous precipitating antibody and the gp70 pigment-associated antigen on melanoma cells which it recognizes. The antibody is useful in detecting pigmented melanoma cells in excised specimen, serum or urine.

Abstract: A radiolabeled or enzyme labeled peptide having no more than 60 amino acids in the chain of the peptide; the peptide having covalently linked amino acids disposed in a steric configuration which is recognized by and bound by an antibody. The labeled peptides can be utilized in various processes to detect the presence of a given antibody or antigen in a sample. Hepatitis B surface antigen and antibody to same may be so detected.

Abstract: Protein immunoassay or purification using an antibody raised to an antigen comprising a polypeptide corresponding to a fragment (but not all) of the protein being assayed; the antibody is capable of binding separately both to the protein as a whole and to the polypeptide; that antibody is used to purify or (with a known concentration of the labeled polypeptide serving as a tracer) to assay the protein.