Phosphorus Attached Indirectly To An Azo Group By Nonionic Bonding Patents (Class 534/727)
  • Patent number: 9234243
    Abstract: The present invention provides novel mutations identified in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that can be used for a more accurate diagnosis of cystic fibrosis (CF) and CF related disorders. Methods for testing a sample obtained from a subject to determine the presence of one or more mutations in the CFTR gene are provided wherein the presence of one or more mutations indicates that the subject has CF or a CF related disorder, or is a carrier of a CFTR mutation.
    Type: Grant
    Filed: May 6, 2014
    Date of Patent: January 12, 2016
    Assignee: Laboratory Corporation of America Holdings
    Inventors: Elizabeth Rohlfs, Deborah Alexa Sirko-Osadsa, Lynne Rosenblum, Narasimhan Nagan, Zhaoqing Zhou, Ruth Heim
  • Publication number: 20150126717
    Abstract: This invention relates to bis-azo colorants for use as bluing agents, laundry care compositions comprising bis-azo colorants that may serve as bluing agents, processes for making such bluing agents and laundry care compositions and methods of using the same. The bluing agents are generally comprised of at least two components: at least one chromophore component and at least one polymeric component. These bluing agents are advantageous in providing a whitening effect to fabrics, while not building up over time and causing undesirable blue discoloration to the treated fabrics.
    Type: Application
    Filed: January 8, 2015
    Publication date: May 7, 2015
    Inventors: Robert L. Mahaffey, JR., Xiaoyong Michael Hong, Eduardo Torres, Dominick J. Valenti, Gregory Scot Miracle
  • Patent number: 9018362
    Abstract: Provided is an oligonucleotide containing an azobenzene derivative, represented by Formula (1) or (2) below: (in the formulae, A1 and A2 each independently represent a hydrogen atom, nucleotide or oligonucleotide, B1 and B2 each independently represent a hydroxyl group, nucleotide or oligonucleotide, R11 and R12 each independently represent a C1-20 alkyl group, R21 and R22 each independently represent a hydrogen atom or C1-20 alkyl group, and R13 to R18 and R23 to R28 each independently represent a hydrogen atom; a C1-20 alkyl group or alkoxy group optionally substituted with a halogen atom, hydroxyl group, amino group, nitro group or carboxyl group; a C2-20 alkenyl group or alkynyl group optionally substituted with a halogen atom, hydroxyl group, amino group, nitro group or carboxyl group; a hydroxyl group; a halogen atom; an amino group; a nitro group; or a carboxyl group).
    Type: Grant
    Filed: July 22, 2011
    Date of Patent: April 28, 2015
    Assignee: National University Corporation Nagoya University
    Inventors: Hiroyuki Asanuma, Xingguo Liang, Hiromu Kashida, Hidenori Nishioka, Taiga Fujii, Teruchika Ishikawa
  • Publication number: 20140123342
    Abstract: The role of extracellular nucleotides and apyrase enzymes in the guard cells that border stomata in regulating stomatal aperture and the plant's resistance to drought and pathogens is disclosed herein. Expression of apyrases APY1 and APY2, in guard cell protoplasts is strongly correlated with cell growth, cell secretory activity and with conditions that favor stomatal opening. Both short-term inhibition of ectoapyrase activity and long-term suppression of APY1 and APY2 transcript levels significantly disrupt normal stomatal behavior in light. Furthermore, two punnoceptor inhibitors in mammals, pyridoxalphosphate-6-azo-phenyl-2?,4?-disulphonic acid (PPADS) and Reactive Blue 2, block ATPS- and ADP?S-induced opening and closing, and also partially block the ability of abscisic acid (ABA) to induce stomatal closure, and light-induced stomatal opening.
    Type: Application
    Filed: June 1, 2012
    Publication date: May 1, 2014
    Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Stanley J. Roux, Iris Steinebrunner, Gregory B. Clark
  • Publication number: 20130225797
    Abstract: Provided is an oligonucleotide containing an azobenzene derivative, represented by Formula (1) or (2) below: (in the formulae, A1 and A2 each independently represent a hydrogen atom, nucleotide or oligonucleotide, B1 and B2 each independently represent a hydroxyl group, nucleotide or oligonucleotide, R11 and R12 each independently represent a C1-20 alkyl group, R21 and R22 each independently represent a hydrogen atom or C1-20 alkyl group, and R13 to R18 and R23 to R28 each independently represent a hydrogen atom; a C1-20 alkyl group or alkoxy group optionally substituted with a halogen atom, hydroxyl group, amino group, nitro group or carboxyl group; a C2-20 alkenyl group or alkynyl group optionally substituted with a halogen atom, hydroxyl group, amino group, nitro group or carboxyl group; a hydroxyl group; a halogen atom; an amino group; a nitro group; or a carboxyl group).
    Type: Application
    Filed: July 22, 2011
    Publication date: August 29, 2013
    Applicant: NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
    Inventors: Hiroyuki Asanuma, Xingguo Liang, Hiromu Kashida, Hidenori Nishioka, Taiga Fujii, Teruchika Ishikawa
  • Publication number: 20130123475
    Abstract: Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection.
    Type: Application
    Filed: September 27, 2012
    Publication date: May 16, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Gregory J. Ewing, Deborah Kay Fieri, Khairuzzaman Bashar Mullah, Ronald Graham
  • Patent number: 8436153
    Abstract: The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
    Type: Grant
    Filed: January 5, 2010
    Date of Patent: May 7, 2013
    Assignee: Biosearch Technologies, Inc.
    Inventors: Tim Carter, Mark Reddington
  • Patent number: 8377641
    Abstract: Disclosed is a group of azo quencher compositions useful as fluorescence quenchers having the general structure of formula 1, methods of making or using the compositions, and kits comprising the composition.
    Type: Grant
    Filed: November 17, 2011
    Date of Patent: February 19, 2013
    Assignee: Integrated DNA Technologies, Inc.
    Inventors: Andrei Laikhter, Mark Aaron Behlke, Joseph Walder, Kevin William Roberts, Yawfui Yong
  • Publication number: 20130029331
    Abstract: The inventors have developed a rapid and sensitive fluorescence-based assay to quantify dNTPs. This assay relies on the principle that incorporation of a limiting dNTP is required for primer-extension and polymerase-mediated 5-3? exonuclease hydrolysis of a quenched fluorophore-labeled probe resulting in fluorescence. The concentration of limiting dNTPs is directly proportional to the fluorescence generated. This assay has important applications in research that investigates the influence of pathological conditions or pharmacological agents on dNTP biosynthesis and regulation.
    Type: Application
    Filed: June 14, 2012
    Publication date: January 31, 2013
    Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA
    Inventors: Peter M. Wilson, Robert D. Ladner
  • Publication number: 20120108799
    Abstract: The invention pertains to modifications for antisense oligonucleotides, wherein the modifications are used to improve stability and provide protection from nuclease degradation. The modifications could also be incorporated into double-stranded nucleic acids, such as synthetic siRNAs and miRNAs.
    Type: Application
    Filed: September 7, 2011
    Publication date: May 3, 2012
    Applicant: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Mark Aaron Behlke, Richard Owczarzy, Yong You, Joseph Alan Walder, Kim Lennox
  • Publication number: 20110282041
    Abstract: The present invention provides a new class of solids supports for synthesis of modified oligomers of nucleic acids, and nucleic acid probes that have a format expediently synthesized on the new supports. Exemplary solid supports include at least one quencher bound through a linker to the solid support. Various exemplary embodiments include a moiety that stabilizes a duplex, triplex or higher order aggregation (e.g., hybridization) of nucleic acids of which the oligomer of the invention is a component. Other components of the solid support include moieties that stabilize aggregations of nucleic acids, e.g., intercalators, minor groove binding moieties, bases modified with a stabilizing moiety (e.g., alkynyl moieties, and fluoroalkyl moieties), and conformational stabilizing moieties, such as those described in commonly owned U.S. Patent Application Publication No. 2007/0059752.
    Type: Application
    Filed: July 28, 2011
    Publication date: November 17, 2011
    Applicant: Biosearch Technologies, Inc.
    Inventors: Ronald M. Cook, Matt Lyttle
  • Publication number: 20110178280
    Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.
    Type: Application
    Filed: August 6, 2010
    Publication date: July 21, 2011
    Applicant: BIOSEARCH TECHNOLOGIES, INC.
    Inventors: RONALD M. COOK, MATT LYTTLE, DAREN DICK
  • Publication number: 20110092683
    Abstract: There are provided compounds of formulas (VA), (VIA), (VIIA) (IXA), (XIA), (XIIA), (XIIIA), and (XIVA): wherein A, Z, R2, X?, and L2 represent various different possibilities. Methods for using such compounds are also provided.
    Type: Application
    Filed: December 21, 2010
    Publication date: April 21, 2011
    Applicant: VALORISATION-RECHERCHE, LIMITED PARTNERSHIP
    Inventors: André Charette, Jean-Christophe Poupon, Alessandro Boezio
  • Publication number: 20110092679
    Abstract: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes.
    Type: Application
    Filed: April 22, 2010
    Publication date: April 21, 2011
    Applicant: Biosearch Technologies, Inc.
    Inventors: Ronald M. Cook, Matt Lyttle, Daren Dick
  • Patent number: 7879986
    Abstract: The specification discloses quenchers of excited state energy, probes and other conjugates comprising the same, and methods for their use. The quenchers of excited state energy have a structure comprising at least one reactive functional group for linking the quencher to a carrier molecule and at least three radicals covalently linked via exocyclic diazo bonds, the at least three radicals including at least one comprising a julolidine of the nominal formula: and the remainder of the at least three radicals being selected from the group of substituted and unsubstituted aryls, substituted and unsubstituted heteroaryls, and combinations thereof.
    Type: Grant
    Filed: February 3, 2006
    Date of Patent: February 1, 2011
    Assignee: Berry & Associates, Inc.
    Inventors: David A. Berry, William H. Pearson
  • Publication number: 20100311184
    Abstract: Azo heterocyclic compounds that are substantially non-fluorescent or only weakly fluorescent useful as energy acceptors. The chemically reactive compounds possess utility for labeling a wide variety of substances, including biomolecules with the resulted conjugates being highly useful for a variety of energy-transfer assays and applications. The biological conjugates of the disclosure exhibit little or no observable fluorescence and their fluorescence is significantly increased upon a biological stimulation.
    Type: Application
    Filed: June 1, 2010
    Publication date: December 9, 2010
    Applicant: AAT BIOQUEST, INC.
    Inventors: Zhenjun DIWU, Jingfang LIAO
  • Patent number: 7758861
    Abstract: The present invention discloses dye-sulfenate derivatives and their bioconjugates for dual phototherapy of tumors and other lesions. The compounds of the present invention may contain either a mixture of Type 1 and Type 2 agents or a single entity that integrates both units in the same molecules. The compounds are designed to produce both Type 1 and Type 2 phototherapeutic effect at once using dual wavelength light source that will produce singlet oxygen and free radicals at the lesion of interest.
    Type: Grant
    Filed: March 21, 2006
    Date of Patent: July 20, 2010
    Assignee: Mallinckrodt Inc.
    Inventors: Raghavan Rajagopalan, Samuel I. Achilefu, Joseph E. Bugaj, Richard D. Dorshow
  • Publication number: 20100112306
    Abstract: A process for printing an image on a substrate comprising applying to the substrate an ink comprising a medium and a mono azo compound of Formula (1) or a salt thereof: wherein: A and X are independently optionally substituted aryl or heteroaryl; B is independently optionally substituted arylene or heteroarylene, in either case comprising a phenylene ring which is attached to the —N?N— and —NRa— groups in the compound of Formula (1) in the para positions; Ra and Rb are independently H, optionally substituted alkyl or optionally substituted aryl; ?the compound of Formula (1) or a salt thereof is a water-soluble dye; and the ink is applied to the substrate by means of an ink jet printer.
    Type: Application
    Filed: September 4, 2007
    Publication date: May 6, 2010
    Applicant: FUJIFILM IMAGING COLORANTS LIMITED
    Inventor: Gavin Wright
  • Publication number: 20100087634
    Abstract: Fluorous-tagged oligonucleotide reagents and an oligonucleotide purification methodology making use thereof, the method comprising: Synthesizing oligonucleotides using oligonucleotide reagents each bearing at least one fluorous group to yield a mixture of synthesis products and reagents, the mixture including at least one target synthesized oligonucleotide bearing at least one fluorous group; passing the mixture through a separation medium having an affinity for the at least one fluorous group so that the target synthesized oligonucleotide bearing at least one fluorous group is adsorbed by the separation medium; washing the separation medium with at least a first solvent to selectively dissociate therefrom substantially all synthesis products and reagents of the heterogenous mixture other than the at least one target synthesized oligonucleotide bearing at least one fluorous group; and subsequently dissociating the at least one synthesized oligonucleotide from the separation medium, with or without the fluorou
    Type: Application
    Filed: October 20, 2009
    Publication date: April 8, 2010
    Applicant: BERRY & ASSOCIATES, INC.
    Inventors: David A. Berry, William H. Pearson
  • Publication number: 20090053821
    Abstract: Disclosed is a group of azo quencher compositions useful as fluorescence quenchers having the general structure of formula 1, methods of making or using the compositions, and kits comprising the composition.
    Type: Application
    Filed: October 16, 2008
    Publication date: February 26, 2009
    Applicant: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Andrei Laikhter, Mark Aaron Behlke, Joseph Walder, Kevin William Roberts, Yawfui Yong
  • Patent number: 7439341
    Abstract: Disclosed is a group of azo quencher compositions useful as fluorescence quenchers having the general structure of formula 1, methods of making or using the compositions, and kits comprising the composition.
    Type: Grant
    Filed: November 12, 2004
    Date of Patent: October 21, 2008
    Assignee: Integrated DNA Technologies, Inc.
    Inventors: Andrei Laikhter, Mark Aaron Behlke, Joseph Walder, Kevin William Roberts, Yawfui Yong
  • Publication number: 20070191313
    Abstract: The invention provides compounds represented by the formula I, each of which compounds may have sphingosine-1-phosphate receptor agonist and or antagonist biological activity: and wherein the variables Y, R4, n, A, X, Z, R1, o, R3, R2 and p are as defined in the specification.
    Type: Application
    Filed: February 15, 2007
    Publication date: August 16, 2007
    Inventors: Richard L. Beard, John E. Donello, Haiqing Yuan, Diana F. Colon, Tien Duong, Xiaoxia Liu, Yihui Hu
  • Patent number: 6902900
    Abstract: The invention comprises novel methods and strategies to detect and/or quantify nucleic acid analytes. The methods involve nucleic acid probes with covalently conjugated dyes, which are attached either at adjacent nucleotides or at the same nucleotide of the probe and novel linker molecules to attach the dyes to the probes. The nucleic acid probes generate a fluorescent signal upon hybridization to complementary nucleic acids based on the interaction of one of the attached dyes, which is either an intercalator or a DNA groove binder, with the formed double stranded DNA. The methods can be applied to a variety of applications including homogeneous assays, real-time PCR monitoring, transcription assays, expression analysis on nucleic acid microarrays and other microarray applications such as genotyping (SNP analysis). The methods further include pH-sensitive nucleic acid probes that provide switchable fluorescence signals that are triggered by a change in the pH of the medium.
    Type: Grant
    Filed: October 21, 2002
    Date of Patent: June 7, 2005
    Assignee: Prolico, LLC
    Inventors: Martin Davies, Ian Bruce, Andreas Wolter
  • Publication number: 20040219576
    Abstract: The present invention relates to compositions and methods for the preparation of modified nucleic acids. In particular, the present invention provides novel reagents and chemistries for the generation of linkers and modified phosphoramidates.
    Type: Application
    Filed: January 6, 2004
    Publication date: November 4, 2004
    Applicant: Third Wave Technologies, Inc.
    Inventors: Zbigniev Skrzypczynski, Sarah R. Wayland
  • Publication number: 20040191796
    Abstract: Oligonucleotide-fluorophore-quencher conjugates wherein the fluorophore moiety has emission wavelengths in the range of about (300) to about (800) nm, and or where the quencher includes a substituted 4-(phenyldiazenyl)phenylamine structure provide improved signal to noise ratios and other advantageous characteristics in hybridization and related assays. The oligonucleotide-fluorophore-quencher conjugates can be synthesized by utilizing novel phosphoramidite reagents that incorporate the quencher moiety based on the substituted 4-(phenyldiazenyl)phenylamine structure, and or novel phosphoramidite reagents that incorporate a fluorophore moiety based on the substituted coumarin, substituted 7-hydroxy-3H-phenoxazin-3-one, or substituted 5,10-dihydro-10 [phenyl]pyrido[2,3-d;6,5-d′]dipyrimidine-2,4,6,8-(1H, 3H, 7H, 9H, 10H)-tetrone structure.
    Type: Application
    Filed: June 25, 2003
    Publication date: September 30, 2004
    Applicant: EPOCH PHARMACEUTICALS, INC.
    Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy
  • Patent number: 6790945
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Grant
    Filed: June 6, 2001
    Date of Patent: September 14, 2004
    Assignee: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M. J. Vermeulen
  • Publication number: 20040137479
    Abstract: The present invention relates to methods for detecting the presence of ribonuclease enzymes, more specifically to methods that provide for a visual detection assay. The methods entail contacting a test sample suspected of containing ribonuclease activity with a substrate containing a ribonuclease-sensitive internucleotide linkage flanked directly or indirectly by a fluorescence reporter group and a dark quencher, such that if a ribonuclease activity is present in the sample, the ribonuclease-sensitive internucleotide linkage is cleaved and the fluorescence reporter group emits a visually detectable signal. The present invention further provides novel nucleic acid compositions used as substrates for such assays and encompasses kits for performing the methods of the invention.
    Type: Application
    Filed: October 27, 2003
    Publication date: July 15, 2004
    Applicant: Integrated DNA Technologies, Inc.
    Inventors: Joseph Alan Walder, Mark Aaron Behlke, Eric Jeffrey Devor, Lingyan Huang
  • Publication number: 20040081959
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Application
    Filed: March 29, 2002
    Publication date: April 29, 2004
    Applicant: Epoch Biosciences, Inc.
    Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M.J. Vermeulen
  • Publication number: 20040023216
    Abstract: A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions.
    Type: Application
    Filed: May 10, 2002
    Publication date: February 5, 2004
    Applicant: Board of Trustees of the University of Illinois
    Inventors: Yi Lu, Juewen Liu
  • Publication number: 20040019190
    Abstract: Invented are non-peptide TPO mimetics. Also invented are novel processes and intermediates used in the preparation of the presently invented compounds. Also invented is a method of treating thrombocytopenia, in a mammal, including a human, in need thereof which comprises administering to such mammal an effective amount of a selected hydroxy-1-azobenzene derivative.
    Type: Application
    Filed: July 3, 2003
    Publication date: January 29, 2004
    Inventors: Connie J Erickson-Miller, Daniel F. Eppley, Julian Jenkins, Juan I. Luengo, Nannan Liu, Alan T. Price, Anthony N. Shaw, Sophie Visonneau, Kenneth Wiggall
  • Publication number: 20040005607
    Abstract: Bis-diazo,triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides, and polypeptides. The quencher moieties are non-fluorescent and accept energy from fluorescent reporter labels by any energy-transfer mechanism, such as FRET.
    Type: Application
    Filed: April 30, 2003
    Publication date: January 8, 2004
    Inventors: Gregory J. Ewing, Khairuzzaman Bashar Mullah, Ronald J. Graham
  • Publication number: 20030228644
    Abstract: Prodrug compounds capable of permeating a desired biological compartment and releasing a biologically active molecule in active form to effect a therapeutic functional change in the compartment to which it is introduced.
    Type: Application
    Filed: December 10, 2002
    Publication date: December 11, 2003
    Inventor: Randell L. Mills
  • Publication number: 20030175728
    Abstract: Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5′ nuclease digestion.
    Type: Application
    Filed: June 6, 2002
    Publication date: September 18, 2003
    Applicant: Epoch Biosciences, Inc.
    Inventors: Yevgeniy S. Belousov, Irina A. Afonina
  • Publication number: 20030096254
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Application
    Filed: March 29, 2002
    Publication date: May 22, 2003
    Applicant: Epoch Biosciences, Inc.
    Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M.J. Vermeulen
  • Publication number: 20030082547
    Abstract: Bis-diazo,triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides, and polypeptides. The quencher moieties are non-fluorescent and accept energy from fluorescent reporter labels by any energy-transfer mechanism, such as FRET.
    Type: Application
    Filed: August 27, 2001
    Publication date: May 1, 2003
    Inventors: Gregory J. Ewing, Khairuzzaman Bashar Mullah, Ronald J. Graham
  • Publication number: 20030060453
    Abstract: Pharmaceutical compositions and compounds are provided. The compounds of the invention demonstrate anti-proliferative activity, and may promote apoptosis in cells lacking normal regulation of cell cycle and death. In one embodiment of the invention, pharmaceutical compositions of the compounds in combination with a physiologically acceptable carrier are provided. The pharmaceutical compositions are useful in the treatment of hyperproliferative disorders, which disorders include tumor growth, lymphoproliferative diseases, angiogenesis. The compounds of the invention are substituted pyrazoles and pyrazolines.
    Type: Application
    Filed: February 15, 2002
    Publication date: March 27, 2003
    Inventors: Zaihui Zhang, Timothy Scott Daynard, Serguei V. Sviridov, Mikhail A. Chafeev, Shisen Wang
  • Publication number: 20030040608
    Abstract: An azo dye represented by the following general formulae (10) and (12): 1
    Type: Application
    Filed: June 24, 2002
    Publication date: February 27, 2003
    Inventors: Keiichi Tateishi, Toshiki Fujiwara, Makoto Yamada
  • Publication number: 20030008304
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Application
    Filed: February 26, 2002
    Publication date: January 9, 2003
    Applicant: Epoch Biosciences, Inc.
    Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M.J. Vermeulen
  • Publication number: 20020177136
    Abstract: A chemical moiety including a polymer (P), a first tethering element (T), a ligand (L) which is a specific sequence of PNA, a second tethering element (T′) and a quencher (Q) is disclosed. In the absence of a complement to the PNA sequence, the PNA is in a tightly coiled configuration, thereby quenching the polymer due to the close proximity of the quencher to the polymer. When a receptor is added that recognizes the PNA sequence, a hybridization of the PNA sequence separates the polymer and the quencher, resulting in an increase of detected fluorescence. The same chemistry is advantageously employed in a competitive assay. A method for detecting nucleic acids in a target sample using the PTLT′Q molecule is also disclosed.
    Type: Application
    Filed: August 23, 2001
    Publication date: November 28, 2002
    Inventors: Duncan W. McBranch, David G. Whitten, Michael Egholm
  • Publication number: 20020155443
    Abstract: In an embodiment, a novel DNA-interactive compound is formed by coupling an alkali metal ion binding moiety with a DNA interactive moiety. An alkali metal ion binding moiety is any group capable of binding alkali metal ions (e.g., lithium, sodium, potassium, etc.). The DNA-interactive moiety is a group of atoms or functionality capable of covalently modifying DNA, through, for example, alkylation, cleavage, metalation, hydrolysis, or crosslinking.
    Type: Application
    Filed: January 25, 2001
    Publication date: October 24, 2002
    Inventors: Sean M. Kerwin, Mark M. McPhee
  • Publication number: 20020106653
    Abstract: Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.
    Type: Application
    Filed: June 27, 2001
    Publication date: August 8, 2002
    Applicant: Nat' l Inst. of advan. Industrial Science and Tech
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku
  • Publication number: 20020103169
    Abstract: Novel anticancer agents of the phenanthridine class having a specific mechanism of action and cancer therapy methods are described, including the novel chemical compounds and their therapeutic use thereof in humans.
    Type: Application
    Filed: August 8, 2001
    Publication date: August 1, 2002
    Inventors: Patrick Mailliet, Jean-Francois Riou, Jean-Louis Mergny, Abdelazize Laoui
  • Publication number: 20020052343
    Abstract: The present invention provides novel compounds of Formula I: 1
    Type: Application
    Filed: December 14, 2000
    Publication date: May 2, 2002
    Inventors: Darin Arthur Allen, Danny Peter Claude McGee, Jeffrey R. Spencer
  • Publication number: 20020034754
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Application
    Filed: June 6, 2001
    Publication date: March 21, 2002
    Inventors: Michael W. Reed, Eugeny Alexander Lukhtanov, Alexander A. Gall, Robert O. Dempcy, Nicolaas M.J. Vermeulen
  • Patent number: 6117986
    Abstract: Compounds are described comprising pyrimidines substituted at the C-5 position with a linker and quencher. These compounds when incorporated into hairpin oligonucleotides quench the fluorescence of fluorophores linked to the 5'-terminus of the oligonucleotide. These nucleotide-quencher compounds are also easy to incorporate into oligonucleotides using conventional, automated, oligonucleotide synthetic techniques.
    Type: Grant
    Filed: June 10, 1998
    Date of Patent: September 12, 2000
    Assignee: Intergen Company, L.P.
    Inventors: Glenn Nardone, Irena Nazarenko, Jila Boal
  • Patent number: 5795970
    Abstract: A disazo dye composition comprising at least a disazo dye represented by the formula (I) and a disazo dye represented by the formula (II): ##STR1## (wherein X and Y indicate an alkoxy group having 1 to 4 carbon atoms, and M indicates hydrogen or a cation selected from the group consisting of, alkaline metal, NH.sub.4, alkyl-substituted ammonium, alkanolammonium, morpholinium and piperidinium), wherein, when the disazo dye composition is analyzed by liquid chromatography, the area ratio of the peak area (Sp) of the disazo dye represented by the formula (I) to the peak area (Sc) of the disazo dye represented by the formula (II), that is Sp:Sc is from 1:0.4 to 1:2.4. This disazo dye composition provides a high quality image, rapid ink fixing and the like even on the plain paper.
    Type: Grant
    Filed: August 22, 1996
    Date of Patent: August 18, 1998
    Assignees: Orient Chemical Industries, Ltd., Seiko Epson Corporation
    Inventors: Takashi Ono, Tatsuya Yagyu, Yoshihiro Sawatari, Masaki Sasaki, Miharu Kanaya, Hiroko Hayashi, Akio Owatari, Yasuhiro Ouki
  • Patent number: 5616174
    Abstract: An ink composition is provided which enables high-quality recording on plain paper and can record an image having excellent water resistance. The ink composition comprises a dye represented by the following formula (I): ##STR1## wherein M represents an unsubstituted or substituted ammonium, R.sup.1 represents COOM, R.sup.2 represents --P(=O)n--(OM).sub.2, where n is 0 or 1, R.sup.3 represents SO.sub.3 M, R.sup.4 and R.sup.5 independently represent H, an alkyl group, or an alkoxyl group, R.sup.6 represents H, a phenyl group which may be substituted, an alkylcarbonyl group, or an alkyl group which may be substituted with a hydroxyl or alkoxyl group, and m is 0 or 1.
    Type: Grant
    Filed: December 4, 1995
    Date of Patent: April 1, 1997
    Assignee: Seiko Epson Corporation
    Inventors: Miharu Kanaya, Akio Owatari, Junko Takatsuna, Masahiro Yatake, Hiroko Hayashi, Takashi Ono, Yoshihiro Sawatari, Tatsuya Yagyu
  • Patent number: 5567597
    Abstract: The present invention provides compounds that function as hydrolytic enzyme inhibitors (inactivators) and substrates. These compounds are useful in assays to detect and measure levels of hydrolytic enzyme activity and are more particularly useful in treatment regimens for various disease states and conditions implicating the underlying specific hydrolytic enzyme. Examples of hydrolytic enzymes include, but are not limited to, phospholipases, lipases, esterases, proteases, etc.
    Type: Grant
    Filed: February 14, 1995
    Date of Patent: October 22, 1996
    Assignee: The Regents of the University of California
    Inventors: Edward A. Dennis, William N. Washburn
  • Patent number: 5308766
    Abstract: The present invention provides compounds that function as hydrolytic enzyme inhibitors (inactivators) and substrates. These compounds are useful in assays to detect and measure levels of hydrolytic enzyme activity and are more particularly useful in treatment regimens for various disease states and conditions implicating the underlying specific hydrolytic enzyme. Examples of hydrolytic enzymes include, but are not limited to, phospholipases, lipases, esterases, proteases, etc.
    Type: Grant
    Filed: June 10, 1991
    Date of Patent: May 3, 1994
    Assignee: The Regents of the University of California
    Inventors: Edward A. Dennis, William N. Washburn
  • Patent number: 5290347
    Abstract: The present invention provides a novel salt-forming type dye represented by the formula: ##STR1## wherein D is a residue of a monoazo or disazo dye, m is an integer of 1 to 3, n is an integer of 1 to 4, and M.sup.+ is a cation selected from the group consisting of cations represented by the formulas: ##STR2## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 are the same or different and represent hydrogen, an alkyl group, an alkoxyalkyl group, a cyclohexyl group, an aralkyl group or a hydroxyalkyl group, and at least one of R.sup.1 to R.sup.4 is a branched or non-branched alkyl, alkoxyalkyl, cycloalkyl, aralkyl or polyoxyalkylene group having not less than 4 carbon atoms, ##STR3## wherein R.sup.5, R.sup.6, R.sup.7 and R.sup.8 are the same or different and represent hydrogen, an alkyl group, or a substituted or non-substituted phenyl group, and at least one of R.sup.5 to R.sup.8 is a substituted or non-substituted phenyl group, and ##STR4## wherein R.sup.9 and R.sup.
    Type: Grant
    Filed: April 9, 1992
    Date of Patent: March 1, 1994
    Assignee: Orient Chemical Industries, Ltd.
    Inventors: Takashi Ono, Tatsuya Yagyu, Katsumi Fukuda