Abstract: The present disclosure relates to freeze-tolerant transgenic Eucalyptus, including methodology for making and products produced there from. Following exposure to cold temperature, and compared to a control plant, the freeze-tolerant Eucalyptus displays a freeze-tolerant phenotype characterized by at least one of increased height, less leaf damage, less dieback of main stem, less crown defoliation, and/or improved tree stem form.

Abstract: A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids from the algal biomass, and esterifying the neutral lipids with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method further includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock.

Abstract: Provided herein are systems and methods for extracting lipids and/or producing biofuel from algae in marine and freshwater environments, wherein algae and bivalves are co-cultured in a system of enclosures comprising water that comprises recycled nutrients that are essential for algal growth. The system also include enclosures for culturing fishes which are used to harvest the algae.

Abstract: Embodiments of the present disclosure provide for novel strategies to harvest algal lipids using mollusks which after feeding algae from the growth medium can convert algal lipids into their biomass or excrete lipids in their pseudofeces which makes algae harvesting energy efficient and cost effective. The bioconverter, filter-feeding mollusks and their pseudofeces can be harvested and converted to biocrude using an advanced thermochemical liquefaction technology. Methods, systems, and materials are disclosed for the harvest and isolation of algal lipids from the mollusks, molluscan feces and molluscan pseudofeces.

Abstract: Processes and methods of recovering desired products from fermentation stillage are presented, including processes and methods of recovering lipids and aqueous materials.

Abstract: Provided herein are systems and methods for sustainable aquaculture. The methods provided herein allows recovery and/or recycling of autochthonous nutrients in fish farming, and recovery of allochthonous nutrients present in eutrophic water. The systems provided herein comprises two closely spaced cages, an array of rotary panels that regulates the flow of matters between the two cages; and a means for producing a directional water current.

Abstract: The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

Abstract: The present invention contemplates the creation of a low fluoride oil processed from a phospholipid-protein complex (PPC) formed immediately upon a crustacean (i.e., for example, krill) catch. The process comprises disintegrating the crustaceans into smaller particles, adding water, heating the result, adding enzyme(s) to hydrolyze the disintegrated material, deactivating the enzyme(s), removing solids from the enzymatically processed material to reduce fluoride content of the material, separating and drying the PPC material. Then, using extraction with supercritical CO2 and ethanol as solvents, inter alia krill oil is separated from the PPC. In the extraction the krill oil can be separated almost wholly from the feed material. The products have low fluoride content. The manufacturing costs in the extraction process are relatively low.

Abstract: The invention provides for identification and use of certain chloroplast transit peptides for efficient processing and localization of dicamba monooxygenase (DMO) enzyme in transgenic plants. Methods for producing dicamba tolerant plants, methods for controlling weed growth, and methods for producing food, feed, and other products are also provided, as well as seed that confers tolerance to dicamba when it is applied pre- or post-emergence.

Abstract: Provided herein are systems and methods for producing biofuel from microalgae that use a population of zooplankton to harvest microalgae in a culture. The methods further comprise gathering the zooplankton, extracting lipids from the zooplankton, and processing the lipids to form biofuel. The systems provided herein comprise at least one enclosure comprising microalgae, means for making the microalgae available to a population of zooplankton, and means for gathering the zooplankton.

Abstract: This invention features a method of producing a triglyceride solution. The method includes contacting a liquid fatty acid alkyl ester and a substance containing triglyceride so that the triglyceride is dissolved into the fatty acid alkyl ester to form a triglyceride solution.

Abstract: Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

Abstract: Lipids can be extracted from a microbial biomass that constitutes at least 20% lipids by weight and has a moisture content of less than 4% by weight by applying pressure to the biomass so as to release lipids therefrom, thereby leaving a biomass of reduced lipid content; and collecting the lipids.

Abstract: A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, sequentially adding solvent sets to the algal biomass, and sequentially separating solid biomass fractions from liquid fractions to arrive at a liquid fraction comprising neutral lipids. The method also includes esterifying the neutral lipids, separating a water miscible fraction comprising glycerin from a water immiscible fraction comprising fuel esters, carotenoids, and omega-3 fatty acids. The method also includes obtaining a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock.

Abstract: The present invention relates to a method for purification of lipid material originating from biological material. In the method the lipid material comprising acylglycerols and phosphorus impurities and at least one added nonpolar solvent and at least one added polar solvent is provided into a reaction zone whereby at least a two phase system comprising a nonpolar phase and a polar phase is formed. The phase system is heated in the closed reaction zone under mixing at a temperature from 150° C. to 300° C. and at a pressure wherein said solvents are in subcritical state, preferably of below 100 bar, dependent on the vapor pressure of the selected solvents, until the phosphorus impurity is removed from the polar phase. Sub-sequently, the nonpolar phase including the purified oil comprising acylglycerols is separated and recovered from said phase system.

Abstract: Methods and related systems efficiently and effectively recover a significant amount of valuable, useable oil from byproducts formed during a dry milling process used for producing ethanol. The method may include forming a concentrate from the byproduct and recovering oil from the concentrate. The step of forming the concentrate may include evaporating the byproduct using a multi-stage evaporator, as well as recovering the oil before the final stage of the evaporator. Further, the step of recovering oil from the concentrate may include using a centrifuge and, in particular, a disk stack centrifuge. Other aspects of the invention include related methods and subsystems for recovering oil.

Abstract: Raw material such as food waste is scanned by a metal detector (11) and objects or containments removed, the food waste is then ground or macerated by a disintegrator (13), and passed to a live steam kettle (14), then passed to an in-line heater (15) wherein it is raised to and maintained at 80% for 20 minutes. The product is pumped to a three-phase decanter centrifuge (16) where it is separated into solids, water waste and tallow (oily product). The latter is pumped to storage tank (20), using a centrifugal pump (21), while water waste is discharged to effluent while solids are discharged to a container (18).

Abstract: Production of ester-based fuels such as biodiesel or jet fuel from renewable starting materials such as lignocellulosic material or algae is disclosed. Pulping and saccharification of the renewable starting materials produces carboxylic acids such as fatty acids or rosin acids, which are esterified via a gas sparged, slurry form of heterogeneous reactive distillation to yield ester-based fuels.

Abstract: The use of acoustic focusing together with a non-destructive extraction process for obtaining lipids from oleaginous algae is described. Acoustic focusing can be used to concentrate oleaginous algae before they are subjected to non-destructive extraction, or acoustic focusing can be used as part of the non-destructive extraction process itself by moving the algae from the algae culture to the extracting solvent. The use of acoustic focusing for removing extraneous particulate matter from algae cultures is also described, as well as the use of non-destructive extraction for controlling levels of algae predators.

Abstract: A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, sequentially adding solvent sets to the algal biomass, and sequentially separating solid biomass fractions from liquid fractions to arrive at a liquid fraction comprising neutral lipids. The method also includes esterifying the neutral lipids, separating a water miscible fraction comprising glycerin from a water immiscible fraction comprising fuel esters, carotenoids, and omega-3 fatty acids. The method also includes obtaining a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock.

Abstract: Methods and means to modulate oil content and/or oil yield in plants, such as oilseed rape plants are provided. Increased oil content and/or oil yield can be achieved by reducing the functional level or activity of PARP1, e.g. through reduction of the expression of the parp1 gene.

Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.

Abstract: A method for extracting internal lipids from wool that is substantially free of lanolin, comprising the use of a fluid under supercritical conditions and an agent that can change the polarity of said fluid, selected from methanol and/or ethanol, is disclosed. According to the operating conditions described, the temperature is between 40° C. and 120° C., and the pressure is between 120 bars and 330 bars. The polarity-changing agent represents between 3% and 15% expressed as volume/volume. The inventive method can be used to obtain internal lipids from wool, one of the most significant components of which are ceramides, which can be used in compositions intended to protect human skin against environmental damage.

Abstract: The invention relates to systems and methods for reducing algal biomass in eutrophic water, wherein organism that feed on algae are introduced into the eutrophic water and cultured in the eutrophic water, until the algal biomass is reduced or the organisms have reach desirable size. The body of eutrophic water can be restocked with juveniles after harvesting. The organisms can be fishes and/or shellfishes. The methods further comprising producing biofuel, specialty chemicals, nutraceuticals, food, and/or fish meal from the harvested fish.

Abstract: The present invention relates to methods for the separation of various components from eggs. More particularly, the present invention relates to methods for the separation of proteins and lipids from eggs, including technical eggs (inedible) or edible eggs, yolks or whites, which comprises cross-linking the lipids of eggs with a cross-linking reagent. In an embodiment, the method includes separating the proteins from the cross-linked lipids. In an embodiment, the method includes the separation of various components associated with the cross-linked lipids. The methods disclosed herein allow for the isolation of multiple different components from the egg in an efficient, cost-effective manner without compromising the recovery process of the components or their subsequent utility in various applications or compositions. The compositions and isolated components obtained by the methods of the invention can be used in pharmaceutical, medical, nutritional, cosmetic or health applications.

Abstract: The presently described invention relates to systems for continuously culturing, harvesting, and oil extraction of algal cultures for the production of algae oils.

Abstract: Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization.

Abstract: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Meloidogyne (root knot nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets show significant conservation at the nucleotide level between orthologs from different Meloidogyne species, facilitating genus-wide targeting by RNA interference.

Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.

Abstract: Disclosed herein are methods and processes for the recovery of oleaginous compounds from biomass and in particular biomass comprises photosynthetic microorganisms. Also disclosure are oleaginous compounds obtained using the disclosed methods.

Abstract: A process and an apparatus separate a volatile component from a substance mixture (raw product). The substance mixture is heated, the volatile component is at least partly evaporated and then the vapor formed is condensed. To improve the separating action, the condensate, in the region of the point of condensation, is contacted with a liquid in which the condensate dissolves.

Abstract: Methods and related systems efficiently and effectively recover a significant amount of valuable, useable oil from byproducts formed during a dry milling process used for producing ethanol. The method may include forming a concentrate from the byproduct and recovering oil from the concentrate. The step of forming the concentrate may comprise evaporating the byproduct using a multi-stage evaporator, as well as recovering the oil before the final stage of the evaporator. Further, the step of recovering oil from the concentrate may comprise using a centrifuge and, in particular, a disk stack centrifuge. Other aspects include related methods and subsystems for recovering oil.

Abstract: The present invention relates to a new process for the production of arachidonic acid and/or eicosapentaenoic acid in plants through the co-expression of a ?-12-/?-15-desaturase, ?-9-elongase, ?-8-desaturase and a ?-5-desaturase and a process for the production of lipids or oils having an increased content of unsaturated fatty acids, in particular ?-3 and ?-6 fatty acids having at least two double bonds and a 18 or 20 carbon atom chain length. Preferably the arachidonic acid and eicosapentaenoic acid are produced in at least a 1:2 ratio. The invention furthermore relates to the production of a transgenic plants, preferably a transgenic crop plant, having an increased content of arachidonic acid and/or eicosapentaenoic acid, oils or lipids containing C18- or C20-fatty acids with a double bond in position 5, 8, 9, 11, 12, 14, 15 or 17 of the fatty acid produced, respectively due to the expression of the ?-12-/?-15-desaturase, of the ?-9-elongase, of the ?-8-desaturase and of the ?-5-desaturase in the plant.

Abstract: The present invention relates generally to a process of producing transgenic plants and more particularly transgenic oil palm plants carrying exogenous genetic material which confers on said plants or cells of said plants particular phenotypic traits. The present invention further provides plant parts, reproductive material and plant products from the transgenic plants. The present invention particularly provides transgenic plants and even more particularly transgenic oil plants having beneficial and useful phenotypic characteristics not present at least to the same extent in non-transgenic plants of the same species. The present invention is further directed to oil, and particularly palm oil, having beneficial and desirable characteristics, produced by the transgenic plants made in accordance with the present invention.

Abstract: The present invention relates to methods of producing lipids. In particular, the present invention relates to methods of increasing the level of one or more non-polar lipids and/or the total non-polar lipid content in a transgenic organism or part thereof. In one particular embodiment, the present invention relates to the use of an acyltransferase, for example, a monoacylglycerol acyltransferase (MGAT) to increase the level of one or more non-polar lipids and/or the total non-polar lipid content in plants, plant seed and/or leaves, algae and fungi.

Abstract: The process and apparatus are for removing a solute from a solute-bearing solid product by means of a solvent which remains in liquid state throughout the entire oil extraction process. In one embodiment, the solvent is normally in gaseous state at ambient temperature and pressure values, but is used mainly in liquid state within the method and apparatus of the present invention by maintaining such pressure and temperature values within the apparatus so that the solvent will remain in this liquid state.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV258468. The invention thus relates to the plants, seeds and tissue cultures of the variety CV258468, and to methods for producing a corn plant produced by crossing a corn plant of variety CV258468 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV258468 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV258468.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV385282. The invention thus relates to the plants, seeds and tissue cultures of the variety CV385282, and to methods for producing a corn plant produced by crossing a corn plant of variety CV385282 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV385282 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV385282.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV363541. The invention thus relates to the plants, seeds and tissue cultures of the variety CV363541, and to methods for producing a corn plant produced by crossing a corn plant of variety CV363541 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV363541 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV363541.

Abstract: Systems and methods for improving dense gas solvent extraction of a solute and recovery of the solvent are provided. A pressure of the solvent/solute mixture obtained from an extraction chamber is increased, e.g. with a pump, thereby providing the mixture above saturation conditions. The increased pressure provides greater solubility of the solute and less solvent being vaporized in a heat exchanger. A buildup of solute in the system is reduced, thus improving system longevity. Also, process conditions of the separation process are isolated from those of the extraction process. Accordingly, the process conditions for the separating process are maintained while the process conditions of the extraction chamber vary with ambient temperature, thus saving cost and energy. This isolation also provides an ability to use, in the gas recovery cycle, a heat pump that can be used for many applications and environmental conditions while still using a conventional refrigerant.

Abstract: The present disclosure relates to refining a product from a biomass containing chlorophyll and/or pheophytins. In particular, a method of refining a product (such as a biofuel) from a photosynthetic organism is disclosed. The photosynthetic organism can be a naturally occurring organism or a genetically modified or altered organism. The method of refining comprises removing nitrogen to obtain the desired product.

Abstract: A soybean cultivar designated S090252 is disclosed. The invention relates to the seeds of soybean cultivar S090252, to the plants of soybean S090252, to plant parts of soybean cultivar S090252, and to methods for producing a soybean plant produced by crossing soybean cultivar S090252 with itself or with another soybean variety. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. This invention also relates to soybean cultivars, or breeding cultivars, and plant parts derived from soybean variety S090252, to methods for producing other soybean cultivars, lines or plant parts derived from soybean cultivar S090252, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing the cultivar S090252 with another soybean cultivar.

Abstract: There is described a processor for use in the microwave torrefaction of biomass material which comprises, a micronised biomass char material and a method of producing a biomass char material, and a method of producing L-glucosan.

Abstract: Organic solvent-free processes for obtaining krill oil compositions are disclosed. The processes include a) cooking krill in a cooker vessel for a time and at a temperature sufficient to denature the protein content of the krill and cause a first solid krill fraction and a first liquid krill fraction to be formed while substantially avoiding emulsification of the first solid and first liquid hill fractions; b) removing the first solid and first liquid krill fractions from the cooker vessel at a temperature of at least about 90° C.; c) separating the first solid fraction and the first liquid fraction; and d) obtaining krill oil with neutral enriched from the first liquid fraction, and e) by pressing of first solid fraction to obtain press liquid or a second liquid krill fraction for obtaining hill oil with phospholipids enriched krill oil, the separating and the obtaining steps being carried out without the use of organic solvents. Krill oil compositions made by the process are also disclosed.

Abstract: Medicaments and therapeutic compositions contain (1) phospholipids having 4,7,10,13,16,19-docosahexaenoic acid covalently bound thereto and (2) at least one omega-3 polyunsaturated fatty acid, or at least one pharmaceutically acceptable omega-3 polyunsaturated fatty acid derivative or mixtures thereof, wherein about 25% of the total 4,7,10,13,16,19-docosahexaenoic acid moieties in the composition are covalently bound to phospholipids, and wherein components (1) and (2) are present in amounts effective to support overall neurological health in a subject.

Abstract: The present invention relates to a method and apparatus for purifying crude tall oil. The purification method comprises a first purification step (2) for separating at least solids (8) from the crude tall oil and producing solids free crude tall oil, and after the first purification step (2) a second purification step (4) for separating water (14) from the solids free crude tall oil. The purification apparatus comprises at least one solids separator (3, 5?) for separating at least solids (8) from the crude tall oil and producing solids free crude tall oil, and at least one water separator (5, 9) arranged downstream of the solids separator (3, 5?) in the flow direction of the crude tall oil for separating at least water (14) from the solids free crude tall oil.

Abstract: The invention relates to a novel canola line designated as SCV470336. The invention also relates to the seeds, the plants, and the plant parts of canola line SCV470336 as well as to methods for producing a canola plant produced by crossing canola line SCV470336 with itself or with another canola line. The invention also relates to methods for producing a canola plant containing in its genetic material one or more transgenes and to the transgenic canola plants and plant parts produced by those methods. The invention further relates to canola lines or breeding lines and plant parts derived from canola line SCV470336, to methods for producing other canola lines or plant parts derived from canola line SCV470336 and to the canola plants, varieties, and their parts derived from use of those methods. The invention additionally relates to hybrid canola seeds, plants, and plant parts produced by crossing the line SCV470336 with another canola line.

Abstract: In one aspect of the invention, a method recovers oil from a concentrated byproduct, such as evaporated thin stillage formed during a dry milling process used for producing ethanol. The method includes forming a concentrate from the byproduct and recovering oil from the concentrate. The step of forming the concentrate may comprise evaporating the byproduct. Further, the step of separating the oil from the concentrate may comprise using a centrifuge and, in particular, a disk stack centrifuge. Other aspects of the invention include related methods and subsystems for recovering oil from thin stillage.

Abstract: In one aspect of the invention, a method recovers oil from a concentrated byproduct, such as evaporated thin stillage formed during a dry milling process used for producing ethanol. The method includes forming a concentrate from the byproduct and recovering oil from the concentrate. The step of forming the concentrate may comprise evaporating the byproduct. Further, the step of separating the oil from the concentrate may comprise using a centrifuge and, in particular, a disk stack centrifuge. Other aspects of the invention include related methods and subsystems for recovering oil from thin stillage.

Abstract: The invention relates to vegetable oil products, including palm oil products, containing one or more constituents from a group which includes monoacylglycerols, diacylglycerols, triacylglycerols, carotenes, tocols, phytosterols, squalene and free fatty acids, from vegetable oil sources and a process for the production thereof by using supercritical fluid extraction.