Nitrogen Bonded Directly To The Phosphorus Patents (Class 558/185)
  • Publication number: 20110160082
    Abstract: The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.
    Type: Application
    Filed: January 19, 2011
    Publication date: June 30, 2011
    Inventors: Sam L. WOO, Ron GRAHAM, Jing TIAN
  • Patent number: 7129229
    Abstract: This invention relates to attachment chemistries for binding macromolecules to a substrate surface or to other conjugation targets. More particularly, this invention relates to attachment chemistries involving branched or linear structures having one or more hydrazide attachment moieties for binding the macromolecules to a substrate surface, or for other conjugation reactions. Novel modifying reagents are provided for the introduction of protected hydrazide attachment moieties or precursor forms of such hydrazides to the macromolecule, either as a single hydrazide or as multiple hydrazides.
    Type: Grant
    Filed: August 10, 2001
    Date of Patent: October 31, 2006
    Assignee: Nanogen Recognomics GmbH
    Inventors: Stefan Raddatz, Jochen Müller-Ibeler, Markus Schweitzer, Christoph Brücher, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles H. Greef, Daguang Wang
  • Publication number: 20030225284
    Abstract: A scalable process for purification of phosphorodiamidite includes steps of solubilizing a crude phosphorodiamidite in an apolar organic solvent, contacting the non-polar organic solvent with a polar phase comprising a polar organic solvent to remove impurities from the solubilized phosphorodiamidite, and removing the non-polar organic solvent from the phosphorodiamidite.
    Type: Application
    Filed: April 11, 2002
    Publication date: December 4, 2003
    Inventors: Quanlai Song, Bruce Ross
  • Patent number: 6222032
    Abstract: In a process for C-alkylation or N-alkylation, the improvement comprising conducting the C-alkylation or N-alkylation reaction in the presence of a slightly nucleophilic strong base of the following formula wherein R′1, R′2 and R′3 are individually selected from the group consisting of alkoxy of 1 to 6 carbon atoms and R′ and R″ are individually selected from the group consisting of unsubstituted or substituted alkyl of 1 to 6 carbon atoms, alkoxy of 1 to 6 carbon atoms, cycloalkyl of 3 to 10 carbon atoms, aryl and aralkyl of 1 to 6 alkyl carbon atoms, the substituents being selected from the group consisting of halogen, alkyl of 1 to 6 carbon atoms, alkoxy of 1 to 6 carbon atoms, —CN, —NO2 and dialkylamino of 1 to 6 alkyl carbon atoms, the aryl being selected from the group consisting of unsaturated monocyclic and condensed carbocyclic and heterocyclic with at least one heteroatom selected from the group consisting of nitrogen, oxy
    Type: Grant
    Filed: November 4, 1999
    Date of Patent: April 24, 2001
    Assignees: Societe de Conseils de Recherches d'Applications Scientifiques (SCRAS), Centre National de la Recherche Scientifique (CNRS)
    Inventors: Guy Bertrand, Dennis Bigg, Jean-Bernard Cazaux, Stéphanie Goumri, Olivier Guerret
  • Patent number: 5955453
    Abstract: Membrane-permeant phosphoinositides, including phosphatidylinositol phosphate esters, are described. A membrane-permeant phosphoinositide includes groups that neutralize the charges of the phosphate moieties of the phosphoinositide. A cell can be treated with the membrane-permeant phosphoinositide, which is then absorbed into the cell. The neutralizing groups can be removed intracellularly to afford the charged phosphoinositide.
    Type: Grant
    Filed: April 24, 1998
    Date of Patent: September 21, 1999
    Assignee: The Regents of the University of California
    Inventors: Roger Y. Tsien, Tao Jiang
  • Patent number: 5840917
    Abstract: A phosphorylamide derivative represented by the general formula (I): ##STR1## wherein R represents an amino group that may be substituted, or a salt thereof, possesses potent antibacterial activity against Helicobacter bacterium, especially Helicobacter pylori, and is useful for prevention or treatment of digestive diseases caused by Helicobacter bacterium, solely or in combination with an antacid or an acid secretion inhibitor.
    Type: Grant
    Filed: December 6, 1996
    Date of Patent: November 24, 1998
    Assignee: Takeda Chemical Industries, Ltd.
    Inventors: Satoru Oi, Hideaki Nagaya, Nobuhiro Inatomi, Masafumi Nakao, Hidefumi Yukimasa
  • Patent number: 5420330
    Abstract: Ether variant lipids bound to phosphoramidites are disclosed. These can be used to create oligonucleotides that are linked to stable lipid-like units. For example, dialkyl glycerols are linked to phosphoramidites.
    Type: Grant
    Filed: September 7, 1990
    Date of Patent: May 30, 1995
    Assignee: Pharmacia P-L Biochemicals Inc.
    Inventor: Charles K. Brush
  • Patent number: 5359100
    Abstract: Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.
    Type: Grant
    Filed: August 13, 1993
    Date of Patent: October 25, 1994
    Assignee: Chiron Corporation
    Inventors: Michael S. Urdea, Brian Warner, Joyce A. Running, Janice A. Kolberg, Jennifer M. Clyne, Ray Sanchez-Pescador, Thomas Horn
  • Patent number: 5344930
    Abstract: Compounds of the general formula: ##STR1## are useful as surfactants in the preparation of fluorocarbon emulsions, which can be used as oxygen-carrying blood substitutes, and for many other therapeutic and diagnostic applications. They are further useful in liposomal formulations which are themselves therapeutic agents or provide a vehicle for such agents.
    Type: Grant
    Filed: November 8, 1993
    Date of Patent: September 6, 1994
    Assignee: Alliance Pharmaceutical Corp.
    Inventors: Jean G. Riess, Francois Jeanneaux, Marie-Pierre Krafft, Catherine Santaella, Pierre Vierling
  • Patent number: 5166144
    Abstract: The invention relates to new O-halogenocyclobutyl S-alkyl (di)thiophosphoric acid ester-amides of the general formula (I) ##STR1## in which A represents fluorine or chlorine,B represents hydrogen or alkyl,X represents oxygen or sulphur,R.sup.1 represents hydrogen, alkyl, --COH (formyl) or --CO-alkyl (acyl) which is optionally substituted by halogen,R.sup.2 represents hydrogen or alkyl, andR.sup.3 represents alkyl or alkoxyalkyl,which can be used as agents for combating pests.
    Type: Grant
    Filed: March 20, 1992
    Date of Patent: November 24, 1992
    Assignee: Bayer Aktiengesellschaft
    Inventors: Bernd-Wieland Kruger, Dietmar Bielefeldt, Karl-R. Gassen, Jurgen Hartwig, Wilhelm Stendel, Christoph Erdelen
  • Patent number: 5164383
    Abstract: O-Halogenocyclopentyl S-alkyl (di)thiphosphoric(phosphonic) acid ester derivatives of the general formula (I) ##STR1## in which R.sup.1 represents alkyl, alkoxy or the radical ##STR2## R.sup.3 represents hydrogen or alkyl and R.sup.4 represents hydrogen, alkyl, --COH (formyl) or optionally halogen-substituted --CO-alkyl (acyl),R.sup.2 represents alkyl or alkoxyalkyl andX represents oxygen or sulphur,can be used as pesticides.
    Type: Grant
    Filed: March 20, 1992
    Date of Patent: November 17, 1992
    Assignee: Bayer Aktiengesellschaft
    Inventors: Bernd-Wieland Kruger, Michael Negele, Jurgen Hartwig, Christoph Erdelen, Wilhelm Stendel
  • Patent number: 4966987
    Abstract: 1-Arylalkoxy-tris(dialkylamino)phosphonium salts of the formula I ##STR1## wherein the substituents R.sup.1 to R.sup.5 are equal or different and represent hydrogen, alkyl having from 1 to 6 carbon atoms which may be perfluorinated, halogen, alkoxy or alkylthio, each having from 1 to 6 carbon atoms, Y represents hydrogen or a perfluoroalkyl group C.sub.n F.sub.2n+1 having from 1 to 6 carbon atoms, X represents bromine or iodine and "Alkyl" represents an alkyl group of 1 to 3 carbon atoms, at most 3, preferably at most 2, of the groups R.sup.1 to R.sup.5 having a meaning other than hydrogen and the alkyl, alkoxy and alkylthio substituents attached to the aromatic nucleus altogether containing preferably at most 6 carbon atoms, in particular at most 4 carbon atoms.
    Type: Grant
    Filed: January 17, 1989
    Date of Patent: October 30, 1990
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Alfred Kruse, Gunter Siegemund, Axel Schumann, Ingo W. Ruppert, deceased
  • Patent number: 4804654
    Abstract: Compounds of the formula ##STR1## wherein the symbols have assigned meanings, and their use as insecticides and/or miticides.
    Type: Grant
    Filed: March 4, 1987
    Date of Patent: February 14, 1989
    Assignee: E. I. Du Pont de Nemours and Company
    Inventor: Mohamed A. H. Fahmy
  • Patent number: 4739095
    Abstract: The invention concerns derivatives of N,N-bis(2-chloroethyl)-phosphoric acid amide of general formula I ##STR1## wherein X and Y are the same or different and signify oxygen or NR.sup.3, R.sup.1 and R.sup.2 are the same or different and R.sup.1 signifies the radical CH.sub.2 (OR.sup.4)--CH.sub.2 --CH.sub.2 --, CH.sub.2 (OR.sup.4)--CH(CH.sub.3)--CH.sub.2 -- or CH.sub.2 (OR.sup.4)--CH(OR.sup.5)--CH.sub.2 -- and, when X is an NR.sup.3 group, can also be hydrogen, R.sup.2 possesses one of the meanings of R.sup.1 or is ethyl, propyl, allyl, propenyl, chloroethyl, methyl or benzyl, R.sup.3 is hydrogen, methyl, ethyl, propyl or allyl and wherein the radicals R.sup.4 and R.sup.
    Type: Grant
    Filed: April 30, 1986
    Date of Patent: April 19, 1988
    Assignee: Max-Planck-Gesellschaft zur Forderung der Wissenschaften E.V.
    Inventor: Hansjorg Eibl
  • Patent number: 4692436
    Abstract: Novel phosphoroimidates of the formula ##STR1## wherein R represents n-propyl or sec.-butyl,R.sup.1 represents methyl or ethyl,R.sup.2 represents methyl or ethyl, andR.sup.3 represents alkyl having from 3 to 4 carbon atoms,and the use of the new compounds as insecticides, acaricides and nematicides.
    Type: Grant
    Filed: May 9, 1986
    Date of Patent: September 8, 1987
    Assignee: Nihon Tokushu Noyaku Seizo K.K.
    Inventors: Toyohiko Kume, Shinichi Tsuboi, Kunihiro Isono
  • Patent number: 4581350
    Abstract: The invention relates to new organic phosphoric acid esters represented by the general formula ##STR1## wherein X represents O, S or NH,Y represents O or S,R.sup.1 represents a lower alkyl group,R.sup.2 represents a lower alkyl group or a lower alkoxy-lower alkyl group,R.sup.3 represents a fluoro-substituted lower alkyl group,Z represents a lower alkyl group, a lower alkoxy group or a halogen atom, andn represents 0 or 2,which can be used as pesticidally active agents.
    Type: Grant
    Filed: May 18, 1984
    Date of Patent: April 8, 1986
    Assignee: Nihon Tokushu Noyaku Seizo K.K.
    Inventors: Junichi Saito, Akio Kudamatsu, Toyohiko Kume, Shinichi Tsuboi
  • Patent number: 4576935
    Abstract: Dihalovinylphenyl phosphates of the formula I ##STR1## where A and B are each halogen or trihaloalkyl, X is oxygen or sulfur, Y and Z are each oxygen, sulfur or --NH--, R.sup.1 is alkyl, R.sup.2 is alkyl, haloalkyl, alkoxyalkyl or alkylthioalkyl, and R.sup.3, R.sup.4 and R.sup.5 independently of one another are each hydrogen, halogen, nitro, alkyl, cyano, alkoxy, alkoxyalkoxy, alkylthioalkylthio, alkoxyalkyl, alkylthioalkyl, alkylthio, trihaloalkyl or trihaloalkoxy, and pesticides containing these compounds.
    Type: Grant
    Filed: October 24, 1983
    Date of Patent: March 18, 1986
    Assignee: BASF Aktiengesellschaft
    Inventors: Gerd-Ulrich Schwarz, Heinrich Adolphi