Abstract: A batch process for the conversion of organic solid waste material containing cellulose and lignin through liquid thermophilic aerobic digestion comprises comminuting the organic solid waste material, adjusting the moisture content of the comminuted material to at least 90% parts by weight of water, mixing the comminuted material with a thermophilic aerobic micro-organism, adjusting the pH of the mixture to 5.5-7.5 and supplying heat and oxygen to the mixture to effect thermophilic aerobic digestion of the mixture. The thermophilic aerobic digestion of the mixture is terminated after the mixture has stabilized as a low nitrogen material but prior to digestion of the cellulose and lignin in the mixture, and the stabilized mixture is separated into solid and liquid fractions. The process includes the monitoring of parameters for determining the termination of digestion.

Abstract: Flocculated bacterial cells are utilized as high-density crop inoculants and delivery systems for one or more agriculturally beneficial microorganisms. The flocculated form of bacteria, such as Azospirillum and Rhizobium, possess superior survivability under storage, as a seed coating and mixed seed priming media. Other microorganisms may be co-flocculated with the aforementioned bacteria, thereby providing a crop inoculant for delivery of multiple agriculturally beneficial microorganisms.

Abstract: A process for producing a dried bacterial agricultural product having superior viability initially involves culturing (fermenting) a species of microorganism in a growth medium to form a culture. The culture containing the microorganism plus the growth medium is then mixed with a carrier. The resulting mixture is incubated for at least one day to increase the microorganism count in the mixture. The mixture is then air dried slowly for at least about one day so the moisture level in the microorganisms is gradually reduced to form the dried composition. The process may further include steps of milling and then coating seeds with the composition. An inoculant composition according to the invention, which may be made by the foregoing process, consists essentially of a clay carrier and at least 10.sup.9 viable bacteria per gram of the composition. Compositions containing biocidal microbes which combat insects, fungi or the like, and other useful microbes, such as growth promoting bacteria, may also be prepared.

Abstract: This invention has for its object to provide a matrix which finds application as a plant culture medium which is light-weight, possessed of large moisture and fertilizer holding capacities, easy to dispose of after use, and benign to the environment or a microorganism-immobilizing support capable of immobilizing a large population of microorganisms with remarkably improved long-term viability and providing for markedly improved colonization and growth rates. The matrix of this invention is composed predominantly of polysaccharide and lignin and has an internal pore structure.

Abstract: The invention relates to a process for developing plants of novel type being capable of nitrogen-fixation also in their leaves. The process of the invention comprises inoculating plant protoplasts, cells, tissues, embryos or organs grown and/or treated under in vitro conditions with bacteria belonging to the family of Azotobacteraceae, then cocultivating the thus-obtained culture at a temperature of 15.degree. to 35.degree. C. and, if desired, propagating and/or regenerating the whole plant under in vitro conditions on or in a culture medium containing nitrogen and main carbon source(s) utilizable only by the plant cells as well as optionally other additives. The process of the invention ensures a well-balanced growth of the plant together with the bacteria.

Abstract: A method for processing a liquid nitrogen-rich organic waste product, in particular a manure product, to an aqueous fertilizer solution utilizes a biological conversion process so as to obtain a biologically stable fertilizer solution. This conversion process includes at least a nitrification step wherein nitrifiable ammonium nitrogen is converted in nitrate nitrogen and, only if the fraction to be nitrified has a too high content of nitrifiable nitrogen, a denitrification step. The fertilizer solution is separated off after the nitrification step so that it contains nitrate and only up to 150 mg NH.sub.4.sup.+ -N/l at most. The fertilizer solution can be used as leaf nutrition or in a hydroponic system.

Abstract: A microbial fertilizer that constitutes a symbiotic association of several recombinant microbial species is described. The fertilizer contains four streptomyces strains and two yeast strains. The streptomyces strains include a nitrogen fixing strain, a phosphorus decomposer, a potassium decomposer and a coal waste decomposer. The yeast strains produce growth factors and energy required by the streptomyces.

Abstract: A bacterial inoculum including a rhizospheric bacterium with increased dicarboxylic acid membrane permease activity.

Abstract: Fertilizer containing microorganisms of the rhizosphere packaged by microencapsulation in a polysaccharide matrix. The inoculum obtained after microencapsulation can be mixed with a granular solid fertilizer, then stored, or with a liquid fertilizer immediately before spreading.

Abstract: A phytohormone for inducing root hair curling and root nodulation in the roots of leguminous plants particularly in the absence of nitrogen-fixing bacteria. The phytohormone comprises a pentasaccharide having a fatty acid condensed on the non-reducing end. A method for treating the roots of leguminous plants for inducing root hair curling and root nodulation is also disclosed.

Abstract: The present invention relates to mutant strains of Bradyrhizobium japonicum that are characterized in an increased nitrogen fixation rate and an altered molybdenum metabolism. The mutant strains require higher levels of molybdate than wild-type Bradyrhizobium japonicum for expression of nitrate reductase activity. In addition, the mutant strains express free-living nitrogenase activity in medium treated to remove molybdate, where the wild-type require molybdate supplementation.

Abstract: The invention relates to a soybean seed, lucerne seed or clover seed coated with an inoculant composition comprising a carrier medium, a beneficial microorganism and an adhesive polymeric material. A high average loading of viable organisms per seed is maintained in storage.

Abstract: Soluble, non-crosslinked polysaccharides are used to stabilize microorganisms for use as inoculants in agriculture. Preferably, the polysaccharide is alginate and plant seeds are inoculated. A solution of the polysaccharide and a suspension of the microorganism are mixed to form a composition containing about 0.005% to about 10% of the polysaccharide. The composition can be stored for one week or more before use and the composition may be dried.

Abstract: The chemical compounds that induce expression of nodulation genes of Bradyrhizobium japonicum strains have been identified. The isoflavones daidzein and genistein were identified as soybean exudate components that induce nod-genes. Other isoflavones, including 7-hydroxyisoflavone, 5,7-dihydroxyisoflavone, biochanin A, formononetin and prunetin, were also found to act as inducers. In addition, several flavonoid compounds including 4',7-dihydroxyflavone, apigenin, kaempferol and coumestrol, were also found to act as inducers. Nodulation inducing compositions and a method for selectively activating the expression of a gene under the control of a soybean exudate inducible promoter in B. japonicum in response to these inducer molecules are described. In addition, soybean inoculating compositions containing the nod-gene inducers and inoculating methods employing these compositions are described.

Abstract: A novel approach for the increased dinitrogen fixation of leguminous plants wherein the root nodulation of the leguminous plant is controlled so as to promote nodulation by a desired bradyrhizobial strain and to inhibit nodulation of an undesired bradyrhizobial strain. Novel leguminous plant lines are provided which restrict nodulation by undesired bradyrhizobial strains while allowing nodulation of a desired bradyrhizobial strain. The plant lines are produced by conventional breeding. Recombinant bradyrhizobial strains are also provided which are characterized by a propensity for root nodulation of a predetermined leguminous plant. The recombinant bradyrhizobial strains are produced by genetic engineering.

Abstract: Rhizobia transformants that nodulate and fix nitrogen in non-legumes. The nodulated non-legume plants can be grown without nitrogenous fertilizer and have at least the same or higher protein content, dry matter content and nitrogen content than their non-nodulated counterparts which are fertilized by the addition of nitrogenous fertilizer. The straw remaining after harvesting the nodulated non-legumes is also high in protein content.

Abstract: A method and apparatus are described for providing improved irrigation to vegetation in open areas such as parks and golf courses, including providing improved microbiotic and nutrient supplies to such vegetation in a non-sterilized environment. In its method aspects, the invention comprises combining in an aqueous slurry effective amounts of first and second microorganisms, enzymes and nutrients, allowing the combined microorganisms to grow in a closed reservoir to reach levels of concentrations of the first and second microorganisms effective for irrigation, enhancement and nutrition of vegetation, and then applying the concentrated materials to the vegetation in a water slurry in quantities sufficient to effectively irrigate and provide nutrition to the vegetation and to provide it with protection against harmful microorganisms.

Abstract: A new portable system is disclosed for composting feces and for treating urine includes a composting retainer which includes a composting means for exposing the feces to sufficient air and water to allow the feces to compost, thereby forming a compost product. A urine-treatment retainer with aerobic nitrifying bacteria disposed on a substrate whereby urine is treated by the bacteria, thereby forming a liquid product. A means is available for transporting the portable system.

Abstract: The sequences of a Rhizobium bacteria responsible for competitiveness with respect to plant nodulation have been isolated and permanently transferred to superior nodulating Rhizobium genome. This has resulted in a stable construct that can form a plant inoculant that yields effective nodulation, while reducing the risk of suppression by other bacteria in the environment.

Abstract: The nodulation ability and/or competition characteristics of strains of Rhizobium japonicum is enhanced by incubating the strains in a nodulation and/or competitive enhancing effective amount of one or more soybean lectins, or in composition comprising a nodulation and/or competitiveness enhancing effective amount of such lectins such as soybean seed exudate. An inoculum can be prepared containing a carrier, Rhizobium japonicum and lectin.

Abstract: A biological product for actively producing nitrogen and phosphate enrichment of soil comprising a nitrogen-fixing effective amount of Azospirillum sp. M32, an amount of Bacillus polymixa M7 sufficient to produce polymyxin, an anti-microbial peptide active against Gram negative bacteria which converts phosphorous materials fixed in the soil to which it is applied to a phosphate in plant-available forms, and a nutrient for the bacteria comprising a cellulosic material containing cellulose as an integral component selected from the group consisting of straw, xylan, hemicellulose and lignin, as well as other organic carbon or nitrogen materials.

Abstract: Nodulation gene-inducing factors have been isolated and identified. A number of substituted flavones, some of which are components of exudates and extracts of leguminous plants, have been found to induce expression of certain nodulation genes in Rhizobium strains. Three components of clover extract 7,4'-dihydroxyflavone, 7,4'-dihydroxy-3'methoxyflavone, and 4'-hydroxy-7-methoxyflavone have been identified as nodulation inducers of certain Rhizobium trifolii nod genes. A number of other flavonoids including flavones, flavanones, flavanols and dihydroflavanols, not necessarily found in legume exudates, were also found to induce expression of certain nodulation genes.

Abstract: Plant protoplasts are exposed for interaction with an external agent by exposing the plasma membrane of protoplasts in the root hairs of the plant without release of protoplasts from the plant. This may be achieved by enzymatically degrading the plant cell wall at the apices of root hairs under conditions which expose the plasma membrane whilst maintaining or readily permitting restoration of the essential functional integrity of the plant. Enzymatic degradation of the cell wall of the apices of root hairs is possible in a wide range of crop species and other species and to expose the plasma membrane with partial protoplast release. This enables direct interaction with plasmids, viruses and micro-organisms at the surface of the plasma membrane of their exposed protoplasts, or the fusion of these with isolated protoplasts of other plant systems.

Abstract: This invention is a mixed horticultural medium consisting essentially of natural zeolite particles, which has a unique property of capturing ammonium ions, and has a high nitrogen content as compared with conventionally available horticultural media thereby providing a high germentation percentage and having a nitrogen supply potential to meet requirements of plants with the aid of microorganisms in the soil.

Abstract: To produce organic fertilizer which is effective for sterile agricultural land, a mixture comprising coral reef powder, domestic animal excreta, sewage disposal waste and ground cellulose derived from plant fiber is prepared and it is well mixed in the presence of organic compounds and inorganic salts. Thereafter, it is subjected to cultivating in a multi-bath type cultivating bath for a predetermined period of time while temperature and pH are adjusted properly. Cultivation is achieved with the addition of bacilli including nitrogen fixing bacillus such as Azotobactor vinelandii, Bacillus megaterum, Rhizobium leguminosarm or the like, Trichoderma virde acting as raw fiber decomposing bacillus, candida utilus acting as yeast fungus and green algae, each of which is cultivated under the operating condition of symbiosis cultivation. The pH in the cultivating bath is maintained in the range of 5.5 to 7.5, preferably in the range of 6 to 7.

Abstract: To produce organic fertilizer which is effective for sterile agricultural land, a mixture comprising coral reef powder, domestic animal excreta, sewage disposal waste and ground cellulose derived from plant fiber is prepared and it is well mixed in the presence of organic compounds and inorganic salts. Thereafter, it is subjected to cultivating in a multi-bath type cultivating bath for a predetermined period of time while temperature and pH are adjusted properly. Cultivation is achieved with the addition of bacilli including nitrogen fixing bacillus such as Azotobactor vinelandii, Bacillus megaterum, Rhizobium leguminosarm or the like, Trichoderma virde acting as raw fiber decomposing bacillus, candida utilus acting as yeast fungus and green algae, each of which is cultivated under the operating condition of symbiosis cultivation. The pH in the cultivating bath is maintained in the range of 5.5 to 7.5, preferably in the range of 6 to 7.

Abstract: A method is disclosed for producing novel strains of Rhizobium bacteria for legume inoculants which are both competitive and have high nitrogen fixation characteristics. The method involves first isolating and identifying the naturally predominant strains of the Rhizobium species of interest in a particular locale and then mutagenizing that strain to produce mutant strains which retain the competitiveness and add enhanced nitrogen fixation capabilities.

Abstract: A prototrophic revertant of a Bradyrhizobium japonicum tryptophan auxotroph was isolated and found to fix more nitrogen symbiotically than wild-type bacteria. The increase in nitrogen fixation is due to an increase in nodule mass because of an increase in nodule number. The physiological basis for this improved symbiosis appears to be an alteration of the tryptophan biosynthetic pathway.

Abstract: The present invention provides a method for developing a culture of microorganisms in the rhizosphere of plants by dipping roots of the plants in a hydrated super absorbant polymer containing living microorganisms, thereby adhering the polymer to the roots.

Abstract: A soil and foliar supplement for application to plants to improve plant growth and quality. A method for increasing plant productivity and quality also is disclosed. The supplement includes a quantity of specific microbes and an organic acid, such as humic acid, fulvic acid, and ulvic acid. Trace minerals also can be added. A humectant also can be incorporated with the microbes, organic acid, and trace minerals to provide a matrix to provide an improved moisture and nutrient environment and a greatly increased surface area and cation exchange ability for optimizing microbe growth. Chelated micronutrients may also be added to the supplement or added later to the foliage to improve the effects of the supplement still further. Methods of improving plant productivity and quality by treating the plant with the microbes, organic acids and a naturally balanced formulation of chelated minerals are disclosed.

Abstract: A composite structure having a thickness substantially less than its width such as a sheet is prepared which includes a substantially water-insoluble particulate or fibrous support having a surface energy of at least about 30 dynes per cm to which nitrogen-fixing filamentous blue-green algae heterocyst cells are attached. the support does not have a deleterious effect on the attached algae and is preferably cellulosic or a polyoletin such a polypropylene. The structure may contain a first and second layer and have a plurality of raised, three-dimensional shapes over at least a portion of at least one surface. Preparing the composite structure includes contacting the support with blue-green algae to permit the algae to attach to the support by means of heterocyst cells. The attach cells in a nitrogen deficient environment, fix nitrogen at a rate substantially greater than unattached cells, and have agricultural applications.

Abstract: A mixture of 2-piperidone and an N-acyllactam compound for use as a soil amending agent which is provided in a molar ratio of 2-piperidone to N-acyllactam compound in the range from 0.1:1 to 10:1 is effective in the promotion of plant growth.

Abstract: A composite is prepared which includes a substantially water-insoluble particulate or fibrous support having a surface energy of at least about 19 dynes per cm to which nitrogen-fixing filamentous heterocystous blue-green algae are attached. The support does not have a deleterious effect on the viability of the attached algae, and is preferably polypropylene or cellulosic. Preparing the composite includes contacting the support with blue-green algae to permit the algae to attach to the support by means of heterocyst cells. The attached cells in a nitrogen-deficient environment, fix nitrogen at a rate substantially greater than unattached cells, and have agricultural applications.

Abstract: A method of preparing inocula of low water activity is disclosed. The inocula are prepared by admixing microorganisms with a polymer gel and lowering and maintaining the water activity below 0.3, preferably below 0.1. These inocula are useful in the treatment of plant disease, particularly crown gall and Dutch elm diseases, as well as in the biological control of insects.

Abstract: A method for enhancing nodulation in legumes growing in a growing medium. The method includes the step of placing in the growing medium in the immediate vicinity of the roots of the legume the nodulation of which is to be enhanced an effective quantity of an enhancing bacteria selected from the group consisting of ATCC 53522, enhancing mutants of ATCC 53522, a mixture of such mutants, and a mixture of such mutants with ATCC 53522.

Abstract: A multilayer structure is prepared containing immobilized nitrogen-fixing filamentous blue-green algal heterocyst cells. The cells are attached to a first layer which is a water-insoluble support having a surface energy of about 30 to about 115 dynes per cm. Second and third layers are adjacent and contiguous with first and second surfaces, respectively, of the first layer. At least one of the second and third layers is transparent to actinic radiation. The support may be cellulosic such as wood pulp. The immobilized cells fix nitrogen at a rate which is substantially greater than cells when not immobilized. The structure is useful as a nutrient source for agricultural purposes.

Abstract: A method for making an agriculturally useful inoculant of dried, dormant bacteria is described. To make the inoculant, a suspension of Rhizobium or other bacteria is maintained, substantially separated from its culture medium, at a temperature in the range of about 0.degree.-30.degree. C. for varying periods of time under aseptic conditions. The bacterial suspension is then mixed with a porous chemically inert granular carrier such that the weight ratio of carrier to bacteria is in the range of about 0.5 to 1.0. Finally, the bacteria-carrier mixture is air dried for a period of about 2 to 10 days under aseptic conditions.

Abstract: Mutant strains of Bradyrhizobium japonicum having enhanced nodulation properties were created by transposon mutagenesis of known Bradyrhizobium japonicum strain I-110. The mutant strains grow well in a yeast-extract mannitol medium, produce extracellular polysaccharides at a level greater than the parent strain under appropriate conditions, are capable of growth on a nutirent medium containing a normally inhibitory amount of succinic acid, and contain a 21 Kdalton protein absent from the parent strain. Such strains can be used to inoculate soil in which soybean plants are grown, resulting in improved plant yields.

Abstract: The invention relates to an inoculant composition for plants, comprising a carrier medium, at least one species of microorganism having a beneficial effect on the plants, and a copolymer of (1) vinyl pyrrolidone and (2) vinyl acetate or styrene or substituted styrene.

Abstract: Inocula having a long storage life and improved resistance to temperature and rehydration and a method of preparing them are disclosed. The method of the invention comprises admixing a culture medium containing microorganisms of the genus Rhizobium and at least one carbohydrate source in a polymer gel and then lowering the water activity of the resulting inoculum and maintaining the water activity at less than 0.1.

Abstract: Legumes inoculated with tryptophan catabolic variants (tan variants) of wild-type bradyrhizobia are characterized by an enhanced capacity to fix atmospheric nitrogen as compared to parent strains. Responses of the symbiotic system to the variants include an auxinic stimulation of root growth, an enhancement of nodulation, and an increase in the nitrogen-fixation activities of the nodulated root systems.

Abstract: A novel natural fertilizer and method of its use are provided. The fertilizer acts to increase the yield of food crop producing plants. The fertilizer comprises less than 0.05% of an active agent in a stable carrier that is non-toxic to the plants and the active agent. The active agent is a fungus of the genus Aspergillus. The fertilizer can be used by coating the seeds of the food crop producing plant at or prior to the time of planting by dusting the ends of a cut plant, by dusting the roots of a seedling, or by treating the soil in which the plant will be grown.

Abstract: Cultivation of a microorganism in a nutrient medium including disrupted sea kelp or a sea kelp extract produces a bioactivating fermentation product which promotes growth and yields when applied to plants. A composition containing such bioactivating fermentation product and an acidification extraction product of humic acid is readily absorbed by plant foliage when sprayed on plants.

Abstract: The soil life of herbicidally active thiocarbamates is extended by employing in combination therewith certain organophosphorus compounds having the formula ##STR1## in which R.sup.4 is C.sub.1 -C.sub.4 alkyl, R.sup.5 is selected from the group consisting of C.sub.1 -C.sub.4 alkoxy, C.sub.1 -C.sub.4 cyanoalkylthio, and C.sub.1 -C.sub.4 alkylamino; R.sup.6 is selected from the group consisting of hydrogen, methyl, and carbomethoxy; R.sup.7 is selected from the group consisting of hydrogen and chlorine; and X is oxygen or sulfur.

Abstract: A novel microbial plant growth promoting composition comprising a mixture of a bacteria and algae, and a method of promoting plant growth therewith are disclosed.

Abstract: A novel agriculturally useful composition that includes a seed treated with selected infectivity-cured Hr plasmid-bearing microorganism. Also provided are methods of enhancing root elongation, shoot elongation or root development of selected seeds. These methods include treating a selected seed with the infectivity-cured Hr plasmid-bearing microorganism. In addition, there is provided a method of inducing germination of grass seed that involves treating a grass seed such as Kentucky bluegrass seed with a selected infectivity-cured Hr plasmid-bearing microorganism. Furthermore, there is provided a method of promoting plant growth at a lower soil temperature than is common at the time of planting a seed of the plant. Additionally, there is disclosed an inoculant for increasing nodulation, root mass and shoot mass in a leguminous plant. The inoculant contains a Rhizobium microorganism and a selected infectivity-cured Hr plasmid-bearing microorganism.

Abstract: Novel processes are provided for the embedding of microorganisms within a polymer matrix wherein the matrix comprises a polymer gel having a base of at least one polymer selected from the group of polysaccharides, in which said polymer is at least partially cross-linked. The embedded microorganism products are useful in the inoculation of leguminous and non-leguminous plants in order to increase their nitrogen-fixing potential and nutrition, respectively.

Abstract: A novel agriculturally useful composition that includes a seed treated with a selected infectivity-cured Hr plasmid-bearing microorganism. Also provided are methods of enhancing root elongation, shoot elongation or root development of selected seeds. These methods include treating a selected seed with the infectivity-cured Hr plasmid-bearing microorganism. In addition, there is provided a method of inducing germination of Kentucky bluegrass seed that involves treating Kentucky bluegrass seed with a selected infectivity-cured Hr plasmid-bearing microorganism. Furthermore, there is provided a method of promoting plant growth at a lower soil temperature than is common at the time of planting a seed of the plant. Additionally, there is disclosed an inoculant for increasing nodulation, root mass and shoot mass in a leguminous plant. The inoculant contains a Rhizobium microorganism and a selected infectivity-cured Hr plasmid-bearing microorganism.

Abstract: My testing procedures have demonstrated that thiophosphoryl triamide (I) and its linear thermal polymers (II) having the structures illustrated below are highly effective inhibitors of urease activity in agricultural soil systems.

Abstract: The invention relates to an inoculant composition for plants, comprising a carrier medium, at least one species of microorganism having a beneficial effect on the plants, and a copolymer of (1) vinyl pyrrolidone and (2) vinyl acetate or styrene or substituted styrene.