Method Of Chemically, Radiologically, Or Spontaneously Mutating A Plant Or Plant Part Without Inserting Foreign Genetic Material Therein Patents (Class 800/276)
  • Patent number: 6995015
    Abstract: The invention features a method of producing a grape somatic embryo having resistance to a plant pathogen, the method including the steps of (a) culturing a grape somatic embryo in a first liquid culture medium that includes a plant growth regulator and a phytotoxin from a plant pathogen; (b) exchanging the first liquid culture medium for a second liquid culture medium not including the phytotoxin; (c) recovering a living grape cell or grape cell cluster from the second liquid culture, the living cell or cell cluster being resistant to the pathogen; and (d) culturing the grape cell or grape cell cluster in a third culture medium to produce a grape somatic embryo.
    Type: Grant
    Filed: May 12, 2000
    Date of Patent: February 7, 2006
    Assignee: University of Florida
    Inventors: Jayasankar Subramanian, Zhijian Li, Dennis J. Gray
  • Patent number: 6891091
    Abstract: The invention relates to a sunflower seed, comprising sunflower oil having increased stearic acid content as compared to wild type seeds preferably between 19.1 and 35% by weight related to the total amount of fatty acids in the oil, obtainable by treating parent seeds with a mutagenic agent during a period of time and in a concentration sufficient to induce one or more mutations in the genetic trait involved in stearic acid biosynthesis resulting in increased production of stearic acid, germinating the treated seeds and culturing progeny plants therefrom, collecting and analyzing progeny seeds, selecting seeds that have acquired the desirable genetic trait and optionally repeating the cycle of germination, culturing and collection of seeds. The invention further relates to oil extracted from the seeds, to a method for preparing the sunflower seeds, a method for preparing such sunflower oil, sunflower plants produced from the seeds and use of the oil.
    Type: Grant
    Filed: February 20, 2001
    Date of Patent: May 10, 2005
    Assignee: Consejo Superior de Investigaciones Cientificas
    Inventors: Enrique Martínez-Force, Valle Fernandez-Moya, Rafael Garcés
  • Patent number: 6835876
    Abstract: A novel garden bean cultivar, designated ‘210944’, is disclosed. The invention relates to the seeds of garden bean cultivar ‘210944’, to the plants of garden bean line ‘210944’ and to methods for producing a bean plant by crossing the cultivar ‘210944’ with itself or another bean line. The invention further relates to methods for producing a bean plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other garden bean lines derived from the cultivar ‘210944’.
    Type: Grant
    Filed: February 4, 2003
    Date of Patent: December 28, 2004
    Assignee: Harris Moran Seed Company
    Inventor: D. Stephen Magnuson
  • Publication number: 20040177398
    Abstract: The invention provides methods for the alteration of fertility in plants through modulation of floral GABA levels. The techniques of the invention may find use in plant breeding techniques. Also provided by the invention are assays for the screening of candidate modulators of GABA metabolism or GABA analogs through the identification of affects on fertility following application of the candidate modulator to a plant. The technique provides an alternative to animal testing and is amenable to large scale application.
    Type: Application
    Filed: June 21, 2002
    Publication date: September 9, 2004
    Inventors: Ravishankar Palaivelu, Laura Brass, Daphne Preuss
  • Patent number: 6774288
    Abstract: The present invention provides grain, seed, feed made from the grain or seed, petfood made from the grain, and food products made from the grain. The grain may be maize grain with the following characteristics: oil burdened, elevated protein content, and low phytate levels. The combination of oil burdened, protein laden, decreased phytate characteristics in grain makes a grain that provides more calories, protein and phosphorus and other nutrients to the feeding animal. Pet foods, and animal feds and corn food products made of the present invention will provide increased nutrition because of the increased bioavailability of the components of the grain.
    Type: Grant
    Filed: January 6, 2000
    Date of Patent: August 10, 2004
    Assignee: BASF Plant Science L.L.C.
    Inventors: Ming-Tang Chang, Peter L. Keeling, Edward P. Wilhelm, Jerry C. Weigel
  • Patent number: 6759569
    Abstract: The present invention relates to a rapid and large-scale production of tomato mutants by utilizing miniature tomato plants which can be crossed with tomato commercial plants. Mutations are induced in the miniature tomato cultivars and desired mutants are subsequently identified in the resulting mutant tomato population.
    Type: Grant
    Filed: June 8, 2000
    Date of Patent: July 6, 2004
    Assignees: Yeda Research and Development Co. Ltd., Yissum Research Development Company of the Hebrew University of Jerusalem
    Inventors: Avraham A. Levy, Rafael Meissner, Yonatan Elkind
  • Patent number: 6730838
    Abstract: The present invention relates to an opiumless and alkaloid-free non-narcotic opium poppy designated as var. Sujata having the following morphological/argonomic features: Days to 50% flowering 100-105 Plant height (cm)  80-100 Peduncle length (cm)  18-20 Peduncle colour Patchy-black No. of capsules/branches per plant  3-4 Capsule shape Flat Capsule surface ˜glabrous (white bloom +) No. of stigmatic rays per capsule  10-12 Shape and size of stigmatic rays slightly small, flattened Latex-flow on incision Absent Seed count per gram of weight 3040-3310 Seed colour Dull-white Seed shape Reniform Seed size Bold (Thickness ++) Seed yield (g/m2) 120-140 Straw yield (g/m2) 135-150 (Capsule hulls) Opium alkaloids in straw Absent.
    Type: Grant
    Filed: March 26, 1999
    Date of Patent: May 4, 2004
    Assignee: Council of Scientific and Industrial Research
    Inventors: Jawahar Ram Sharma, Raj Kishori Lal, Ajai Prakash Gupta, Hari Om Misra, Vasudha Pant, Ram Chandra, Mohd Rashid
  • Patent number: 6696294
    Abstract: The present invention provides methods for generating and identifying mutations in any target gene of a polyploid plant species. In a preferred aspect of the present invention, a plant is constructed and/or selected that has at least one copy of a functional, target gene located exclusively in only one of its homoeologous, or homologous, genomes. Seed derived from the selected plant are then contacted with an effective amount of at least one mutagenic agent, the treated seed are germinated and the seeds or plants derived therefrom, are screened for mutations in the target gene. Thus, the inventive concepts set forth herein can be used to create, select and identify mutations in any target gene of any suitable polyploid plant, thereby providing a source of numerous, readily-identifiable mutations that can, if so desired, be used in crosses to develop unique new crop cultivars.
    Type: Grant
    Filed: December 18, 2000
    Date of Patent: February 24, 2004
    Assignee: Northwest Plant Breeding Co.
    Inventor: Calvin F. Konzak
  • Publication number: 20030182693
    Abstract: Disclosed is a new variety of four-leaf clover named “Lucky Together”, and a method of breeding the same in large quantities. The method of breeding the new variety of four-leaf clover of the present invention comprises the steps of: (a) sterilizing a clover, (b) treating the clover with chemical agent in order to induce mutation and cell division of the clover, (c) roots-inducing of the mutated clover, (d) asexual propagation of the variety of clover, (e) comparing the features of the variety with those of wild-type clover, and (f) identifying a uniformity of the features of the variety. The four-leaf clover according to the present invention has strong nature, strong cold resistance and excellent disease resistance. Furthermore, the clover according to the present invention grows rapidly and well under intensive light, and can compete successfully with weeds. Therefore, the clover can be used not only for feed but also for improving the quality of soil.
    Type: Application
    Filed: February 28, 2003
    Publication date: September 25, 2003
    Inventor: Won-Kyung Lee
  • Patent number: 6583339
    Abstract: A series of independent technical systems including a combination of mutation methods such as cellular mutation, ultraviolet irradiation, X-ray irradiation or the like, formation and use of shoot primordium and selection of mutated cells are employed to produce genetic mutation in Manilagrass and obtain Manilagrass that retains its green leaves in winter while producing substantially no anthocyanins under normal cultivating conditions, Manilagrass that has a high stolon density, and dwarfed Manilagrass; the newly invented completely novel genotypes are used to obtain new Eragrostoideae plants.
    Type: Grant
    Filed: August 23, 2000
    Date of Patent: June 24, 2003
    Assignee: Kaisui Chemical Industry Co., Ltd.
    Inventor: Itsuki Tsunemori
  • Patent number: 6525245
    Abstract: The present invention relates to methods for determining nucleic acid sequences that encode components of signal transduction pathways in higher plants. The method comprises combining a portion of an AOX promoter linked in operable fashion to a reporter gene to detect nucleic acid sequences of components of the signal transduction pathways between mitochondria function and metabolic status and nuclear gene expression and the signal transduction pathways between branched chain amino acid biosynthetic pathways and nuclear gene expression. A polynucleotide that encodes a portion of an AOX promoter, AOX1a, operably linked to a luciferase reporter gene is provided. A recombinant vector, transformed cells, and transformed organisms containing this polynucleotide are disclosed.
    Type: Grant
    Filed: May 22, 2000
    Date of Patent: February 25, 2003
    Assignee: The Board of Regents of the University of Nebraska
    Inventor: David M. Rhoads
  • Patent number: 6521818
    Abstract: A method for producing crucifer germplasm, in particular broccoli and cauliflower germplasm, with high levels of cancer chemoprotectant agents is provided which comprises screening a population of plants for glucosinolate profiles and selecting an individual plant with an enhanced alkyl/indole glucosinolate ratio. Subsequent breeding with a selected plant results in varieties, lines, and plants with desirable glucosinolate profiles. A method of growing crucifer sprouts to enhance chemoprotectant activity of the sprouts by germinating the seeds in a limited water volume is also provided. A method for producing crucifer germplasm which produces seeds with reduced leakage of seed material is provided which comprises screening a population of plants for production of seeds with reduced leakage and selecting an individual plant which produces such seeds.
    Type: Grant
    Filed: June 30, 1999
    Date of Patent: February 18, 2003
    Assignee: John Hopkins School of Medicine
    Inventor: Jed W. Fahey
  • Publication number: 20020184673
    Abstract: The invention relates to a sunflower seed, comprising sunflower oil having increased stearic acid content as compared to wild type seeds preferably between 19.1 and 35% by weight related to the total amount of fatty acids in the oil, obtainable by treating parent seeds with a mutagenic agent during a period of time and in a concentration sufficient to induce one or more mutations in the genetic trait involved in stearic acid biosynthesis resulting in increased production of stearic acid, germinating the treated seeds and culturing progeny plants therefrom, collecting and analyzing progeny seeds, selecting seeds that have acquired the desirable genetic trait and optionally repeating the cycle of germination, culturing and collection of seeds. The invention further relates to oil extracted from the seeds, to a method for preparing the sunflower seeds, a method for preparing such sunflower oil, sunflower plants produced from the seeds and use of the oil.
    Type: Application
    Filed: February 20, 2001
    Publication date: December 5, 2002
    Applicant: Consejo Superior de Investigaciones Cientificas.
    Inventors: Enrique Martinez-Force, Valle Fernandez-Moya, Rafael Garces
  • Publication number: 20020104128
    Abstract: The present invention provides methods for generating doubled haploid and/or haploid plants from microspores. In a presently preferred embodiment of the methods of the present invention, plant material is selected that bears reproductive organs containing microspores at a developmental stage that is amenable to androgenic induction. The microspores are treated by contacting the selected plant material with water and subjecting the selected plant material to temperature stress, and optionally to nutrient stress. Preferably the selected plant material is contacted with an effective amount of a sporophytic development inducer and an effective amount of an auxin and/or a cell spindle inhibiting agent. Optionally, the selected plant material is contacted with an effective amount of a cytokinin and/or an effective amount of a gibberellin. The treated microspores are isolated, preferably by density centrifugation utilizing a solution of 0.
    Type: Application
    Filed: January 8, 2002
    Publication date: August 1, 2002
    Applicant: Northwest Plant Breeding Company
    Inventors: Calvin F. Konzak, Enrique A. Polle, Weiguo Liu, Yuanming Zheng
  • Patent number: 6414222
    Abstract: The present invention provides methods for preparing herbicide tolerant corn plants. Also provided are herbicide tolerant corn plants, as well as seeds and progeny derived from these plants.
    Type: Grant
    Filed: August 30, 1996
    Date of Patent: July 2, 2002
    Assignee: Regents of the University of Minnesota
    Inventors: Burle G. Gengenbach, David A. Somers, Margaret A. Egli, Lorelei C. Marshall, Donald L. Wyse, Shelia M. Lutz, Kevin L. Van Dee, William B. Parker
  • Patent number: 6403863
    Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activties. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.
    Type: Grant
    Filed: May 14, 1999
    Date of Patent: June 11, 2002
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: L. Curtis Hannah, Thomas W. Greene
  • Patent number: 6380466
    Abstract: Rapeseed in plants producing rapeseed which have a yellow-seed coat are provided. The yellow-seed coat is controlled by a single locus mutation. Plants from the rapeseed are useful for transferring the trait into elite lines of canola.
    Type: Grant
    Filed: May 4, 1998
    Date of Patent: April 30, 2002
    Assignee: Calgene LLC
    Inventor: Daniel Facciotti
  • Patent number: 6361935
    Abstract: Broadly, the present invention relates to mutating starch genes in polyploid cereal grains. Specifically, this invention concerns mutant wheat plants, mutant wheat grain and the starch therefrom.
    Type: Grant
    Filed: April 8, 1999
    Date of Patent: March 26, 2002
    Assignee: ExSeed Genetics LLC
    Inventors: Peter L. Keeling, Francie G. Dunlap, Ming Chang
  • Patent number: 6339184
    Abstract: This invention is directed to a screening method for the selection of mutations which confer acetohydroxyacid synthase (AHAS) inhibiting herbicide resistance to wheat. After mutagenesis of wheat seeds, the seeds are soaked in an AHAS inhibiting herbicide-containing solution containing a particular class of AHAS inhibiting herbicide. After planting, the soil containing the seeds is sprayed with an AHAS inhibiting herbicide of the same class as that used in the seed soak step prior to the emergence of the seedlings from the soil. Those wheat seedlings which emerge and are normal in appearance demonstrate resistance to the class of AHAS inhibiting herbicides used in the seed soak and spraying steps. This invention is also directed to the wheat selections and seeds identified by the screening method.
    Type: Grant
    Filed: March 25, 1994
    Date of Patent: January 15, 2002
    Assignee: BASF Aktiengellschaft
    Inventor: Wendy A. Smith
  • Patent number: 6232529
    Abstract: The present invention provides methods of obtaining a greater than normal concentration of oil in the seed of a plant, the method comprising altering the carbon metabolism in the seed leading to an alteration in the partitioning of assimilates from starch biosynthesis to enhance the biosynthesis and accumulation of oil, preferably without significantly affecting the size of the storage organ in a way that adversely impacts the commercial value of the material. Seeds produced by the present methods are also provided.
    Type: Grant
    Filed: November 20, 1996
    Date of Patent: May 15, 2001
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: George Singletary, Paul C. Anderson, Sean J. Coughlan
  • Patent number: 6225533
    Abstract: Brassica seeds having a maximum of glucosinolate content of 3.4 &mgr;moles per gram of seed, plant lines and progeny thereof which produces such seeds, and meal derived from such seeds are disclosed.
    Type: Grant
    Filed: February 1, 1999
    Date of Patent: May 1, 2001
    Assignee: Cargill, Incorporated
    Inventors: Lorin Roger DeBonte, Zhegong Fan
  • Patent number: 6150591
    Abstract: The present invention relates to a novel double flower gene in Verbena which is phenotypically expressed in the formation of flowers with one or more additional petals than existing Verbena cultivars. The present invention also relates to a Verbena seed, a Verbena plant, a Verbena flower, a Verbena variety and a Verbena hybrid which comprise the novel double flower gene. The present invention also relates to a method of producing the disclosed Verbena plants and seeds.
    Type: Grant
    Filed: October 2, 1998
    Date of Patent: November 21, 2000
    Assignee: Goldsmith Seeds, Inc.
    Inventors: Mitchell Eugene Hanes, Stanislaw Nalepa
  • Patent number: 6143963
    Abstract: Broadly, the present invention relates to mutating starch genes in polyploid cereal grains. Specifically, this invention concerns mutant wheat plants, mutant wheat grain and the starch therefrom.
    Type: Grant
    Filed: October 6, 1997
    Date of Patent: November 7, 2000
    Assignee: ExSeed Genetics, L.L.C.
    Inventors: Peter L. Keeling, Francie G. Dunlap, Ming Chang
  • Patent number: 6127602
    Abstract: The present invention provides methods of making paper utilizing glucans, produced by glucosyltransferase D enzymes of the species Streptococcus mutans, instead of modified starches. The present glucans are functionally similar to the hydroxethyl modified starch and are particularly useful in the sizing and coating steps of paper manufacture. The present glucans also exhibit thermoplastic properties and impart gloss to the paper during the coating step. In particular, the present invention provides plant cells and plants transformed with Streptococcus mutans genes encoding wild-type or mutant glucosyltransferase D enzymes.
    Type: Grant
    Filed: January 16, 1998
    Date of Patent: October 3, 2000
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventor: Scott E. Nichols
  • Patent number: 6111168
    Abstract: Single-gene, non-lethal mutations responsible for low phytic acid-containing seeds are selectable by means of a method for assaying seeds which are otherwise phenotypically, or nearly phenotypically, normal. Maize mutants having from 20% to 95% reductions in kernel phytic acid phosphorus compared to the wild-type, without any noticeable reduction in total phosphorus, were isolated by this method. Mutants obtained in accordance with the invention are useful for developing commercial, low phytic acid seed, plant lines.
    Type: Grant
    Filed: March 5, 1997
    Date of Patent: August 29, 2000
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventor: Victor Raboy
  • Patent number: 6084157
    Abstract: Seeds, plants and oils are provided having low FDA saturates; high oleic acid; low linoleic acid; high or low palmitic acid; low stearic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties.
    Type: Grant
    Filed: March 21, 1997
    Date of Patent: July 4, 2000
    Assignee: Cargill Incorporated
    Inventors: Lorin Roger Debonte, Zhegong Fan, Willie Hsiao-Tsu Loh
  • Patent number: 6077994
    Abstract: The CONSTANS (CO) gene of Arabidopsis thaliana and homologues from Brassica napus are provided and are useful for influencing flowering characteristics in transgenic plants, especially the timing of flowering.
    Type: Grant
    Filed: October 20, 1997
    Date of Patent: June 20, 2000
    Assignee: Plant Bioscience Limited
    Inventors: George M. Coupland, Joanna J. Putterill
  • Patent number: 6051752
    Abstract: The invention relates to a genetic male gametophytic factor capable, when expressed, of conferring a defective endosperm phenotype to seeds, said seeds being unable to germinate on their own. The invention also relates to the use of plants or part of plants comprising said factor for carrying out processes for obtaining hybrid seeds and plants.
    Type: Grant
    Filed: July 19, 1996
    Date of Patent: April 18, 2000
    Assignee: Petosluis Recherche France
    Inventors: Annie Sauton, Daniel Gabillard, Yves Gonon
  • Patent number: 5948954
    Abstract: A commercially viable, runner-type peanut cultivar, as well as seeds and plants of the cultivar, agronomically similar to Florunner but having an improved fatty acid profile in the seed oil.
    Type: Grant
    Filed: October 10, 1997
    Date of Patent: September 7, 1999
    Assignees: Agrigenetics, Inc., Hershey Foods Corporation
    Inventors: Michael Eugene Horn, Eric Jon Eikenberry, Juan Enrique Romero Lanuza, James Douglas Sutton
  • Patent number: 5874662
    Abstract: A method for producing somaclonal variant cotton plant. The method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: February 23, 1999
    Assignee: Mycogen Corporation
    Inventors: Thirumale S. Rangan, David M. Anderson
  • Patent number: 5859350
    Abstract: A canola line has been stabilized to produce seeds having an .alpha.-linolenic acid content of less than that of generic canola oil, more preferably less than or equal to about 7% .alpha.-linolenic acid relative to total fatty acid content of said seed and a total glucosinolate content of less than 18 .mu.mol/g of defatted meal, more preferably less than or equal to about 15 .mu.mol/g of defatted meal. This canola line has reduced sulfur content of less than or equal to 3.0 ppm, improved sensory characteristics and increased oxidative stability.
    Type: Grant
    Filed: March 9, 1998
    Date of Patent: January 12, 1999
    Assignee: Cargill Incorporated
    Inventors: Lorin R. DeBonte, Willie H.T. Loh, Zhegong Fan