Tobacco Patents (Class 800/317.3)
  • Patent number: 11008581
    Abstract: Disclosed are rootstock plants, and methods of producing the rootstock plants, characterized by improved grafting success, enhanced root growth and other performance parameters.
    Type: Grant
    Filed: May 21, 2018
    Date of Patent: May 18, 2021
    Assignee: University of Connecticut
    Inventor: Yi Li
  • Patent number: 11000059
    Abstract: The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: May 11, 2021
    Assignee: U.S. SMOKELESS TOBACCO COMPANY LLC
    Inventors: Dongmei Xu, Mark T. Nielsen, Yanxin Shen
  • Patent number: 10595554
    Abstract: The invention provides a method of extracting and isolating certain compounds from tobacco. The resulting isolate can include more than 90% by weight of a given compound and can be used as a flavor component for tobacco material used in smoking articles and smokeless tobacco compositions. Exemplary compounds that may be present in the isolate according to the invention include, but are not limited to, solanone, neophytadiene, megastigmatrienone, ?-damascenone, norsolanadione, cis-abienol, ?-cembratrienediol, ?-cembratrienediol, sucrose esters, and lutein.
    Type: Grant
    Filed: February 1, 2016
    Date of Patent: March 24, 2020
    Assignee: R.J. Reynolds Tobacco Company
    Inventors: Crystal Dawn Hege Byrd, Anthony Richard Gerardi, Michael Francis Dube
  • Patent number: 9464297
    Abstract: The present invention relates to p450 enzymes and nucleic acid sequences encoding p450 enzymes in Nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.
    Type: Grant
    Filed: May 6, 2013
    Date of Patent: October 11, 2016
    Assignee: U.S. Smokeless Tobacco Company
    Inventor: Dongmei Xu
  • Patent number: 9370160
    Abstract: The present invention provides tobacco inbred plants ALBEX1F and ALBEX1MS. The present invention also provides parts of such plants and products made from those parts. The present invention also includes progeny of the provided plants including hybrids.
    Type: Grant
    Filed: February 27, 2012
    Date of Patent: June 21, 2016
    Assignee: Altria Client Services LLC
    Inventors: Dongmei Xu, Mark T. Nielsen, Yanxin Shen
  • Patent number: 9228195
    Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.
    Type: Grant
    Filed: January 30, 2012
    Date of Patent: January 5, 2016
    Assignees: North Carolina State University, University of Kentucky Research Foundation
    Inventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
  • Patent number: 9228194
    Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.
    Type: Grant
    Filed: January 30, 2012
    Date of Patent: January 5, 2016
    Assignees: North Carolina State University, University of Kentucky Research Foundation
    Inventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
  • Patent number: 9137958
    Abstract: Disclosed herein are genetically modified tobacco plants, and tobacco products derived from such plants, comprising an altered uptake and/or altered levels of at least one environmental contaminant or other non-natural chemical as compared to an unmodified parent tobacco plant from which the modified plant is derived. The alteration in uptake and/or levels may be a reduction, or alternatively an increase, in the uptake and/or levels of the environmental contaminant or other non-natural chemical. These methods and plants are useful for improving tobacco products or in generating tobacco lines that may have environmentally useful properties.
    Type: Grant
    Filed: February 8, 2012
    Date of Patent: September 22, 2015
    Assignee: Reynolds Technologies, Inc.
    Inventors: Patsy Elizabeth Elliott, Darlene Madeline Lawson
  • Patent number: 9102948
    Abstract: MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in Nicotiana plants. In particular, suppressing or overexpressing one or more of MPO1 and MPO2 may be used to decrease or increase nicotine and nicotinic alkaloid levels in tobacco plants. Suppression or overexpression of one or more of MPO1 and MPO2 may be used in combination with modification of expression of other genes encoding enzymes on the nicotinic alkaloid biosynthetic pathway such as A622, NBB1, PMT, and QPT.
    Type: Grant
    Filed: November 18, 2007
    Date of Patent: August 11, 2015
    Assignee: 22nd Century Limited, LLC
    Inventors: Takashi Hashimoto, Akira Kato
  • Patent number: 9096864
    Abstract: The present invention provides tobacco inbred plants NCBEX1F and NCBEX1MS, and NC EX90. The present invention also provides parts of such plants and products made from those parts. The present invention also includes progeny of the provided plants including hybrids.
    Type: Grant
    Filed: February 28, 2012
    Date of Patent: August 4, 2015
    Assignee: North Carolina State University
    Inventors: Ramsey S. Lewis, Ralph E. Dewey
  • Publication number: 20150136152
    Abstract: This application provides high yielding tobacco plants with Oriental tobacco aroma and flavor characteristics and development of these plants via breeding. This application also provides hybrid seeds that give rise to high yielding tobacco plants with Oriental tobacco aroma and flavor characteristics. This application provides methods of growing and harvesting high yielding tobacco plants with Oriental tobacco aroma and flavor characteristics. This application further provides tobacco products made from high yielding tobacco plants with Oriental tobacco aroma and flavor characteristics, and methods making such tobacco products.
    Type: Application
    Filed: November 18, 2014
    Publication date: May 21, 2015
    Applicant: Altria Client Services Inc.
    Inventors: Marcos Fernando de Godoy Lusso, Greg Davis, Jerry W. Morris
  • Patent number: 9029656
    Abstract: Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.
    Type: Grant
    Filed: April 8, 2011
    Date of Patent: May 12, 2015
    Assignee: 22nd Century Limited, LLC
    Inventors: Takashi Hashimoto, Akira Kato
  • Publication number: 20150128305
    Abstract: The present invention provides herein a polynucleotide sequence encoding the tubby-like protein, CaTLP1, from chickpea (Cicer arientium L.) that is responsive to abiotic stress and is involved in plant growth and development. Further, the recombinant DNA construct and recombinant vector comprising the polynucleotide sequence encoding CaTLP1, host cell comprising the recombinant vector and a process for producing a transgenic plant that expresses the CaTLP1 protein are also provided herein.
    Type: Application
    Filed: May 7, 2013
    Publication date: May 7, 2015
    Inventors: Niranjan Chakraborty, Subhra Chakraborty, Asis Datta, Vijay Wardhan, Kishwer Jahan
  • Patent number: 9024113
    Abstract: Recombinant constructs and methods useful for improvement of plants are provided. In particular, recombinant constructs comprising promoters functional in plant cells positioned for expression of polynucleotides encoding starch branching enzymes from microbial sources are provided. The disclosed constructs and methods find use in production of transgenic plants to provide plants, particularly crop plants, having improved yield.
    Type: Grant
    Filed: October 29, 2007
    Date of Patent: May 5, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Yongwei Cao, Gregory J. Hinkle, Steven C. Slater, Xianfeng Chen, Barry S. Goldman
  • Publication number: 20150121568
    Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for an Arabidopsis thaliana (AT SVL3) promoter. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
    Type: Application
    Filed: January 15, 2015
    Publication date: April 30, 2015
    Inventors: Mark Alan Chamberlin, Shai Joshua Lawitt
  • Publication number: 20150113679
    Abstract: The present invention provides methods for obtaining plants that exhibit useful traits by perturbation of plastid function in plant rootstocks and grafting the rootstocks to scions. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants that exhibit the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that provide for plastid perturbation are also provided.
    Type: Application
    Filed: September 24, 2014
    Publication date: April 23, 2015
    Inventors: Sally Mackenzie, Michael Fromm, Kamaldeep Virdi, Yashitola Wamboldt
  • Patent number: 9012736
    Abstract: The present invention provides a method for producing plants with a desired phenotypic trait which comprises subjecting plants to mutagenesis, screening chimeric progeny for plants having the desired phenotypic trait, and propagating the survivors. In an embodiment, the phenotypic trait comprises an altered amino acid content. Preferably, the technique is used to generate Nicotiana tobacum plant lines having an increase in at least one amino acid. In a preferred embodiment, the invention provides improved Nicotiana tobacum plant lines producing at least 1.35 nmole of threonine per milligram of dry plant weight. These plants are useful for improving the flavor and aroma of the tobacco.
    Type: Grant
    Filed: July 26, 2010
    Date of Patent: April 21, 2015
    Assignee: Reynolds Technologies, Inc.
    Inventors: Luis Mayan Dominguez, Jerry Wayne Lawson, Wennuan Liu, Michael Francis Dube
  • Publication number: 20150106978
    Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, introns, and/or 3?-UTRs, isolated from Zea mays.
    Type: Application
    Filed: October 15, 2014
    Publication date: April 16, 2015
    Inventors: Manju GUPTA, Navin ELANGO, Karthik N. MUTHURAMAN, Jeffrey BERINGER, Sara BENNETT, Huixia WU
  • Publication number: 20150106971
    Abstract: There is disclosed a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a threonine synthase and having at least 90% sequence identity to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 or at least 87% sequence identity to SEQ ID NO:4, or SEQ ID NO:5; (ii) a polypeptide encoded by any one of said polynucleotides set forth in (i); or (iii) a polypeptide having at least 95% sequence identity to SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8; or (iv) a construct, vector or expression vector comprising the polynucleotide as set forth in (i).
    Type: Application
    Filed: August 31, 2012
    Publication date: April 16, 2015
    Applicant: PHILIP MORRIS PRODUCTS S.A.
    Inventors: Lucien Bovet, Nicolas Sierro
  • Publication number: 20150106977
    Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, and/or 3?-UTRs, isolated from Zea mays.
    Type: Application
    Filed: October 15, 2014
    Publication date: April 16, 2015
    Inventors: Manju GUPTA, Navin ELANGO, Karthik N. MUTHURAMAN, Jeffrey BERINGER, Sara BENNETT, Huixia WU, Shavell GORMAN, Andrew WORDEN
  • Publication number: 20150101078
    Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.
    Type: Application
    Filed: December 15, 2014
    Publication date: April 9, 2015
    Inventors: MARC C ALBERTSEN, Yukoh Hiei, Teruyuki Imayama, Masakazu Kashihara, Norio Kato, Toshihiko Komari, Toshiyuki Komori, Stanley Luck, Ichiro Oka, Hajime Sakai, Yoshimitsu Takakura, Scott V Tingey, Satoru Usami
  • Publication number: 20150096069
    Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, enhanced nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.
    Type: Application
    Filed: March 6, 2013
    Publication date: April 2, 2015
    Inventors: Paolo Castiglioni, Jaishree M. Chittoor-Vijayanath, Barry S. Goldman, Robert J. Meister, Monnanda S. Rajani, G. Ramamohan, Naveen Silvester, Tyamagondlu V. Venkatesh, Jingrui Wu, Xiaoyun Wu
  • Publication number: 20150096083
    Abstract: The present invention provides non-coding regulatory element polynucleotide molecules isolated from the lipid transfer protein (LTP) gene of Oryza sativa and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Oryza sativa regulatory polynucleotide sequences, and methods for preparing and using the same.
    Type: Application
    Filed: July 28, 2014
    Publication date: April 2, 2015
    Inventors: Wei Wu, Qi Wang, James Morrell, Linda Lutfiyya, Maolong Lu, Zhaolong Li, Ping Li, Michelle Lacy, David Kovalic, Yongwei Cao, Andrey Boukharov, Stanislaw Flasinski, Peter T. Hajdukiewicz
  • Publication number: 20150089683
    Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.
    Type: Application
    Filed: March 6, 2013
    Publication date: March 26, 2015
    Inventors: Mark Scott Abad, Pranesh Badami, Amarjit Basra, Erin Bell, Christopher P. Bonin, Paolo Castiglioni, Eric R. Cerny, Jaishree M. Chittoor-Vijayanath, Paul Chomet, Todd Dezwaan, Stephen Duff, Michael D. Edgerton, Barry S. Goldman, Balasulojini Karunanandaa, Kempagangaiah Krishnamurthy, Ping Li, Adrian A. Lund, Llinda Lutfiyya, Thomas M. Malvar, Donald E. Nelson, Monnanda S. Rajani, Dhanalakshmi Ramachandra, Thomas G. Ruff, Sara Salvador, S. Sangeetha, Char Shobha, Jeffrey M. Staub, Virginia Ursin, Tyamagondlu V. Venkatesh, Kammaradi R. Vidya, Xiaoyun Wu, Sean Yang, Jianmin Zhao
  • Publication number: 20150082494
    Abstract: The present invention provides for a genetically modified eukaryotic host cell comprising (a) a gene encoding a first nucleotide sugar transporter (NST) operably linked to a promoter, wherein the gene and/or the promoter is heterologous to the cell, and/or (b) a native gene encoding a second NTS is disrupted and/or a promoter of the native gene is disrupted. Such modified cells can be altered in the production of polysaccharide and/or glycopeptides. The present invention also provides for methods of making or using such modified cells.
    Type: Application
    Filed: June 16, 2014
    Publication date: March 19, 2015
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Henrik Vibe Scheller, Carsten Rautengarten, Berit Ebert, Joshua L. Heazlewood
  • Publication number: 20150075548
    Abstract: The present invention relates to genetic constructs, which can be used in the preparation of transgenic plants. The constructs can have the ability of reducing nitrate concentration in the plant, in particular the plant's leaves, and for inducing a senescence-like phenotype. The invention extends to plant cells transformed with such constructs, and to the transgenic plants themselves. The invention also relates to methods of producing transgenic plants, and to methods of reducing nitrate content in plants. The invention also relates to harvested plant leaves, for example tobacco leaves, that have been transformed with the genetic constructs, and to various tobacco articles, such as smoking articles, comprising such harvested plant leaves.
    Type: Application
    Filed: March 19, 2013
    Publication date: March 19, 2015
    Inventors: Susan Davenport, Pascaline Le Lay, Juan Pablo Sanchez Tamburinno
  • Publication number: 20150075547
    Abstract: The present invention relates to genetic constructs, which can be used in the preparation of transgenic plants. The constructs can have the ability of reducing nitrate concentration in the plant, in particular the plant's leaves, and for inducing a senescence-like phenotype. The invention extends to plant cells transformed with such constructs, and to the transgenic plants themselves. The invention also relates to methods of producing transgenic plants, and to methods of reducing nitrate content in plants. The invention also relates to harvested plant leaves, for example tobacco leaves, that have been transformed with the genetic constructs, and to various tobacco articles, such as smoking articles, comprising such harvested plant leaves.
    Type: Application
    Filed: March 19, 2013
    Publication date: March 19, 2015
    Inventors: Pascaline LE LAY, Juan-Pablo SANCHEZ TAMBURRINO, Susan DAVENPORT
  • Publication number: 20150080553
    Abstract: The invention provides methods for reducing the levels of alfa (1,3)-fucosylated N-glycans on glycoproteins produced in plants or plant cells. In addition, the invention provides alfa(1,3)-fucosyltransferase genes from Nicotiana benthamiana, and mutant N. benthamiana plants in which the levels of alfa(1,3)-fucosylated N-glycans are reduced.
    Type: Application
    Filed: October 4, 2012
    Publication date: March 19, 2015
    Inventors: Koen Weterings, Gerben Van Eldik
  • Patent number: 8975471
    Abstract: The invention provides methods and compositions useful in target sequence suppression, target sequence validation and target sequence down regulation. The invention provides polynucleotide constructs useful for gene silencing or RNA down regulation, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence or to down regulate RNA.
    Type: Grant
    Filed: October 12, 2005
    Date of Patent: March 10, 2015
    Assignee: The Rockefeller University
    Inventors: Jose L. Reyes Taboada, Xiuren Zhang, Takashi Soyano, Nam-Hai Chua, Qi-Wen Niu, Shih-Shun Lin
  • Publication number: 20150067919
    Abstract: The present invention relates to a genetically modified plant having an increased amount of oil in its green biomass as compared to the oil in the green biomass of its non-genetically modified counterpart. The plants may be used for producing bio-fuels such as biodiesel fuel.
    Type: Application
    Filed: August 5, 2014
    Publication date: March 5, 2015
    Inventors: Vyacheslav ANDRIANOV, Mykola BORSYUK
  • Publication number: 20150059010
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell. Breeding methods and methods for selecting plants utilizing a two component RNA polynucleotide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: August 20, 2014
    Publication date: February 26, 2015
    Inventors: Andrew Mark Cigan, Phillip A. Patten, Joshua K. Young
  • Publication number: 20150047070
    Abstract: A method of producing improved drought tolerance in a plant by introducing into a plant or plant part a vector having a nucleic acid molecule which expresses or increases a polypeptide that confers drought tolerance compared to a plant in which no vector with the nucleic acid is introduced. The polypeptide is a NON-RACE-SPECIFIC DISEASE RESISTANCE 1 (NDR1).
    Type: Application
    Filed: March 8, 2013
    Publication date: February 12, 2015
    Applicant: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY
    Inventors: Brad Day, Patricia Santos, Caleb Knepper
  • Publication number: 20150034110
    Abstract: The present invention relates to genetic constructs, which can be used in the preparation of transgenic plants. The constructs can have the ability of reducing nitrate concentration in the plant, in particular the plant's leaves. The invention extends to plant cells transformed with such constructs, and to the transgenic plants themselves. The invention also relates to methods of producing transgenic plants, and to methods of reducing nitrate content in plants. The invention also relates to harvested plant leaves, for example tobacco leaves, that have been transformed with the genetic constructs, and to various tobacco articles, such as smoking articles, comprising such harvested plant leaves.
    Type: Application
    Filed: March 19, 2013
    Publication date: February 5, 2015
    Inventors: Susan Davenport, Pascaline Le Lay, Juan Pablo Sanchez Tamburrino
  • Publication number: 20150040272
    Abstract: The present disclosure describes genetically-modified plants having enhanced tolerance to multiple abiotic stressors, such as extreme temperatures (heat or cold) and/or drought. Abiotic stress tolerance is enhanced by ectopic expression of a heterologous glutaredoxin. Abiotic stress tolerance (particularly drought) is also enhanced by inhibited function, activity, or expression of an endogenous glutaredoxin. Methods of producing such genetically-modified plants are also disclosed.
    Type: Application
    Filed: August 31, 2012
    Publication date: February 5, 2015
    Applicants: BAYLOR COLLEGE OF MEDICINE, KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
    Inventors: Sunghun Park, Frank White, Jungeun Park, Kendal Hirschi, Ning-Hui Cheng
  • Patent number: 8940963
    Abstract: Process of producing in a plant, in plant tissue, or in plant cells a hetero-oligomeric protein comprising at least a first and a second protein subunit, said process comprising expressing in plant cells at least said first and said second protein subunit by (i) providing to said plant, said plant tissue or said plant cells a plus-sense single-stranded RNA viral vector encoding at least said first and said second protein subunit or (ii) providing to said plant, said plant tissue or said plant cells a first and a second plus-sense single-stranded RNA viral vector, said first viral vector encoding at least said first protein subunit, said second viral vector encoding at least said second protein subunit, whereby at least said first viral vector and said second viral vector are non-competing viral vectors.
    Type: Grant
    Filed: January 27, 2006
    Date of Patent: January 27, 2015
    Assignee: Icon Genetics GmbH
    Inventors: Anatoly Giritch, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
  • Patent number: 8940962
    Abstract: The present invention provides novel promoters for use in plants. Specifically, the present invention provides novel chimeric promoters comprising combinations of plant viral enhancer elements and plant promoters. The present invention also provides DNA constructs; transgenic cells, plants, and seeds containing these novel chimeric promoters; and methods for preparing and using the same.
    Type: Grant
    Filed: September 21, 2009
    Date of Patent: January 27, 2015
    Assignee: Monsanto Technology LLC
    Inventor: Stanislaw Flasinski
  • Publication number: 20150026840
    Abstract: To produce a bacterial microcompartment shell, or a designed shell based on naturally occurring bacterial microcompartment shells in a new host organism, a synthetic operon is constructed that contains the desired shell protein genes and translation efficiency is controlled by host specific ribosomal binding sites. Proteins or other molecules can be encapsulated in the microcompartment shells by various methods described herein. The constructs can also be used to express self-assembling sheets comprised of shell proteins.
    Type: Application
    Filed: March 14, 2014
    Publication date: January 22, 2015
    Applicant: The Regents of the University of California
    Inventors: Cheryl A. Kerfeld, Jonathan K. Lassila, James N. Kinney
  • Publication number: 20150026841
    Abstract: The present invention relates to an isolated COP1 nucleic acid sequence from a maize plant and the isolated COP1 nucleic acid sequence is named as ZmCOP1. The present invention also relates to a method of using the ZmCOP1 nucleic acid sequence to control the shade avoidance response of a crop plant for high density farming and yield enhancement.
    Type: Application
    Filed: June 25, 2014
    Publication date: January 22, 2015
    Inventors: Nordine Cheikh, Molian Deng, Philip W. Miller, Nanfei Xu
  • Publication number: 20150026845
    Abstract: Compositions and methods for modifying the production levels of alkaloids in plants are provided. Alkaloid production can be genetically controlled by modulating the transcriptional activation of PMT genes mediated by members of the ERF family and/or Myc family of transcription factors. Novel nucleotide sequences encoding the Myc family of transciption factors are also provided.
    Type: Application
    Filed: June 23, 2014
    Publication date: January 22, 2015
    Inventors: Michael Paul Timko, Paul J. Rushton, Sheng-Cheng Han, Hongbo Zhang, Marta Tatiana Bokowiec
  • Publication number: 20150020241
    Abstract: The present invention provides non-coding regulatory element polynucleotide molecules isolated from the S-adenosyl methionine synthetase (SAMS3) gene of Arabidopsis thaliana and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Arabidopsis thaliana regulatory polynucleotide sequences, and methods for preparing and using the same.
    Type: Application
    Filed: June 6, 2014
    Publication date: January 15, 2015
    Inventors: Wei Wu, Qi Wang, Michael Edgerton, Kim Beazley, Jill Deikman, Michael W. Petersen, Bei Zhang
  • Publication number: 20150013705
    Abstract: Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.
    Type: Application
    Filed: July 23, 2014
    Publication date: January 15, 2015
    Inventors: Takashi HASHIMOTO, Akira Kato
  • Publication number: 20150013706
    Abstract: Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.
    Type: Application
    Filed: July 28, 2014
    Publication date: January 15, 2015
    Inventors: Takashi HASHIMOTO, Akira Kato
  • Patent number: 8933297
    Abstract: The invention provides Nicotiana benthamiana mutant plants which are incapable of forming xylosyl-structures on glycoproteins. In addition, the invention provides methods for the production of heterologous glycoproteins in said mutant plants.
    Type: Grant
    Filed: June 10, 2010
    Date of Patent: January 13, 2015
    Assignee: Icon Genetics GmbH
    Inventors: Koen Weterings, Gerben Van Eldik
  • Publication number: 20150013033
    Abstract: The present invention provides an expression cassette containing a polynucleotide coding sequence for a hydroxycinnamoyl-CoA hydratase-lyase (HCHL), which is operably linked to a heterologous promoter. Also provided are methods of engineering plants that have reduced lignification, as well as cells, plant parts, and plant tissues from such engineered plants.
    Type: Application
    Filed: July 13, 2012
    Publication date: January 8, 2015
    Applicant: The Regents of the University of California
    Inventors: Dominique Loqué, Amyerick Guillaume Eudes
  • Publication number: 20150013031
    Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.
    Type: Application
    Filed: June 3, 2014
    Publication date: January 8, 2015
    Inventors: Larry A. Gilbertson, Xudong Ye
  • Publication number: 20150007362
    Abstract: The relationship between F-box proteins and proteins involved in the ethylene response in plants is described. In particular, F-box proteins may bind to proteins involved in the ethylene response and target them for degradation by the ubiquitin/proteasome pathway. The transcription factor EIN3 is a key transcription factor mediating ethylene-regulated gene expression and morphological responses. EIN3 is degraded through a ubiquitin/proteasome pathway mediated by F-box proteins EBF1 and EBF2. The link between F-box proteins and the ethylene response is a key step in modulating or regulating the response of a plant to ethylene. Described herein are transgenic plants having an altered sensitivity to ethylene, and methods for making transgenic plant having an altered sensitivity to ethylene by modulating the level of activity of F-box proteins. Methods of altering the ethylene response in a plant by modulating the activity or expression of an F-box protein are described.
    Type: Application
    Filed: November 27, 2013
    Publication date: January 1, 2015
    Applicant: Salk Institute for Biological Studies
    Inventors: Hongwei Guo, Joseph R. Ecker
  • Publication number: 20150007363
    Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.
    Type: Application
    Filed: July 1, 2014
    Publication date: January 1, 2015
    Inventors: L. CURTIS HANNAH, THOMAS W. GREENE
  • Publication number: 20140380524
    Abstract: Provided herein is a transgenic plant, seed, or progeny, genetically engineered to overexpress one or more exogenous 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGS1) in an amount effective to enhance growth and/or seed yield relative to a control plant. Also provided are methods of enhancing plant growth and/or seed yield by genetically engineering a plant to overexpress one or more exogenous HMGS1 in an amount effective to enhance growth and/or seed yield relative to a control plant. The plant belongs to the Solanaceae family, and the one or more exogenous HMGS1 comprise an amino acid sequence at least 77% identical to Brassica juncea HMGS1 as set forth in SEQ ID NO:6. Further provided are methods of screening for a functional variant of Brassica juncea HMGS1.
    Type: Application
    Filed: April 24, 2014
    Publication date: December 25, 2014
    Inventors: Mee Len Chye, Pan Liao, Hui Wang, Mingfu Wang
  • Patent number: 8916746
    Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.
    Type: Grant
    Filed: October 29, 2010
    Date of Patent: December 23, 2014
    Assignees: Japan Tobacco, Inc., E. I. du Pont de Neumours and Company, Pioneer Hi-Bred International, Inc.
    Inventors: Masakazu Kashihara, Toshiyuki Komori, Ichiro Oka, Satoru Usami, Norio Kato, Yukoh Hiei, Yoshimitsu Takakura, Toshihiko Komari, Teruyuki Imayama, Scott V. Tingey, Hajime Sakai, Marc C. Albertsen, Stanley Luck
  • Publication number: 20140373198
    Abstract: The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.
    Type: Application
    Filed: August 18, 2014
    Publication date: December 18, 2014
    Inventors: Patrick S. SCHNABLE, Sudhansu DASH