Tobacco Patents (Class 800/317.3)
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Patent number: 11970702Abstract: The present invention generally relates to methods and materials involved in producing tobacco plants having reduced levels of conversion of nicotine to nornicotine. In certain embodiments, the invention is directed to mutations in a nicotine demethylase gene, tobacco plants comprising mutations in a nicotine demethylase gene, and tobacco compositions and products thereof. In other embodiments, the invention is directed toward nicotine demethylase RNA interference, tobacco plants comprising a nicotine demethylase RNA interference transgene, and tobacco compositions and products thereof.Type: GrantFiled: April 13, 2022Date of Patent: April 30, 2024Assignee: U.S. Smokeless Tobacco Company LLCInventors: Dongmei Xu, Mark T. Nielsen, Yanxin Shen
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Patent number: 11597941Abstract: Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that positively regulates alkaloid biosynthesis, such as nicotine biosynthesis. In particular, the present disclosure provides methods for the inhibition of Nicotiana benthamiana auxin response factor 1 (NbTF1) to reduce alkaloid biosynthesis in plants.Type: GrantFiled: February 26, 2021Date of Patent: March 7, 2023Assignee: 22nd Century Limited, LLCInventors: Jonathan Page, Andrea T. Todd
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Patent number: 11535874Abstract: Provided herein is a non-naturally occurring microbial organism (NNOMO) having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.Type: GrantFiled: October 21, 2013Date of Patent: December 27, 2022Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Patent number: 11499160Abstract: A genetically modified plant or plant cell with reduced ?1,3-fucosyltransferase and ?1,2-xylosyltransferase activity compared to a wild type plant or plant cell, wherein less than 10% of the total glycan on a protein produced by the plant or plant cell is ?1,3-fucosylated glycan and less than 3% of the total glycan on the protein is ?1,2-xylosylated glycan is provided. In one embodiment, the plant or plant cell comprises three T-DNA insertions expressing five copies of RNAi targeting ?1,3-fucosyltranserase and three copies of RNAi targeting ?1,2xylosyltransferase.Type: GrantFiled: November 29, 2017Date of Patent: November 15, 2022Assignee: PlantForm CorporationInventors: Michael D. McLean, Zacharie LeBlanc
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Patent number: 11452275Abstract: This disclosure provides a number of sequences involved in the transport of alkaloids from the root to the leaf in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences, and tobacco products made from such plants.Type: GrantFiled: August 28, 2020Date of Patent: September 27, 2022Assignee: Altria Client Services LLCInventors: Jesse Frederick, Yanxin Shen, Dongmei Xu, Ujwala Warek, James Arthur Strickland
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Patent number: 11332754Abstract: Methods for improving transgene in chloroplasts are disclosed along with improved transgenes so produced and methods of use thereof for the treatment of disease. Specifically, the methods comprising analyzing the native sequence of a nucleic acid encoding a protein of interest and replacing codons in said sequence with those preferentially used in psbA genes in chloroplasts in higher plants.Type: GrantFiled: March 20, 2017Date of Patent: May 17, 2022Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIAInventor: Henry Daniell
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Patent number: 11008581Abstract: Disclosed are rootstock plants, and methods of producing the rootstock plants, characterized by improved grafting success, enhanced root growth and other performance parameters.Type: GrantFiled: May 21, 2018Date of Patent: May 18, 2021Assignee: University of ConnecticutInventor: Yi Li
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Patent number: 11000059Abstract: The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.Type: GrantFiled: March 12, 2019Date of Patent: May 11, 2021Assignee: U.S. SMOKELESS TOBACCO COMPANY LLCInventors: Dongmei Xu, Mark T. Nielsen, Yanxin Shen
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Patent number: 10595554Abstract: The invention provides a method of extracting and isolating certain compounds from tobacco. The resulting isolate can include more than 90% by weight of a given compound and can be used as a flavor component for tobacco material used in smoking articles and smokeless tobacco compositions. Exemplary compounds that may be present in the isolate according to the invention include, but are not limited to, solanone, neophytadiene, megastigmatrienone, ?-damascenone, norsolanadione, cis-abienol, ?-cembratrienediol, ?-cembratrienediol, sucrose esters, and lutein.Type: GrantFiled: February 1, 2016Date of Patent: March 24, 2020Assignee: R.J. Reynolds Tobacco CompanyInventors: Crystal Dawn Hege Byrd, Anthony Richard Gerardi, Michael Francis Dube
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Patent number: 9464297Abstract: The present invention relates to p450 enzymes and nucleic acid sequences encoding p450 enzymes in Nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.Type: GrantFiled: May 6, 2013Date of Patent: October 11, 2016Assignee: U.S. Smokeless Tobacco CompanyInventor: Dongmei Xu
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Patent number: 9370160Abstract: The present invention provides tobacco inbred plants ALBEX1F and ALBEX1MS. The present invention also provides parts of such plants and products made from those parts. The present invention also includes progeny of the provided plants including hybrids.Type: GrantFiled: February 27, 2012Date of Patent: June 21, 2016Assignee: Altria Client Services LLCInventors: Dongmei Xu, Mark T. Nielsen, Yanxin Shen
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Patent number: 9228195Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: GrantFiled: January 30, 2012Date of Patent: January 5, 2016Assignees: North Carolina State University, University of Kentucky Research FoundationInventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
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Patent number: 9228194Abstract: Compositions and methods for reducing the level of nornicotine and N?-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.Type: GrantFiled: January 30, 2012Date of Patent: January 5, 2016Assignees: North Carolina State University, University of Kentucky Research FoundationInventors: Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
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Patent number: 9137958Abstract: Disclosed herein are genetically modified tobacco plants, and tobacco products derived from such plants, comprising an altered uptake and/or altered levels of at least one environmental contaminant or other non-natural chemical as compared to an unmodified parent tobacco plant from which the modified plant is derived. The alteration in uptake and/or levels may be a reduction, or alternatively an increase, in the uptake and/or levels of the environmental contaminant or other non-natural chemical. These methods and plants are useful for improving tobacco products or in generating tobacco lines that may have environmentally useful properties.Type: GrantFiled: February 8, 2012Date of Patent: September 22, 2015Assignee: Reynolds Technologies, Inc.Inventors: Patsy Elizabeth Elliott, Darlene Madeline Lawson
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Patent number: 9102948Abstract: MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in Nicotiana plants. In particular, suppressing or overexpressing one or more of MPO1 and MPO2 may be used to decrease or increase nicotine and nicotinic alkaloid levels in tobacco plants. Suppression or overexpression of one or more of MPO1 and MPO2 may be used in combination with modification of expression of other genes encoding enzymes on the nicotinic alkaloid biosynthetic pathway such as A622, NBB1, PMT, and QPT.Type: GrantFiled: November 18, 2007Date of Patent: August 11, 2015Assignee: 22nd Century Limited, LLCInventors: Takashi Hashimoto, Akira Kato
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Patent number: 9096864Abstract: The present invention provides tobacco inbred plants NCBEX1F and NCBEX1MS, and NC EX90. The present invention also provides parts of such plants and products made from those parts. The present invention also includes progeny of the provided plants including hybrids.Type: GrantFiled: February 28, 2012Date of Patent: August 4, 2015Assignee: North Carolina State UniversityInventors: Ramsey S. Lewis, Ralph E. Dewey
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Publication number: 20150136152Abstract: This application provides high yielding tobacco plants with Oriental tobacco aroma and flavor characteristics and development of these plants via breeding. This application also provides hybrid seeds that give rise to high yielding tobacco plants with Oriental tobacco aroma and flavor characteristics. This application provides methods of growing and harvesting high yielding tobacco plants with Oriental tobacco aroma and flavor characteristics. This application further provides tobacco products made from high yielding tobacco plants with Oriental tobacco aroma and flavor characteristics, and methods making such tobacco products.Type: ApplicationFiled: November 18, 2014Publication date: May 21, 2015Applicant: Altria Client Services Inc.Inventors: Marcos Fernando de Godoy Lusso, Greg Davis, Jerry W. Morris
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Patent number: 9029656Abstract: Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.Type: GrantFiled: April 8, 2011Date of Patent: May 12, 2015Assignee: 22nd Century Limited, LLCInventors: Takashi Hashimoto, Akira Kato
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Publication number: 20150128305Abstract: The present invention provides herein a polynucleotide sequence encoding the tubby-like protein, CaTLP1, from chickpea (Cicer arientium L.) that is responsive to abiotic stress and is involved in plant growth and development. Further, the recombinant DNA construct and recombinant vector comprising the polynucleotide sequence encoding CaTLP1, host cell comprising the recombinant vector and a process for producing a transgenic plant that expresses the CaTLP1 protein are also provided herein.Type: ApplicationFiled: May 7, 2013Publication date: May 7, 2015Inventors: Niranjan Chakraborty, Subhra Chakraborty, Asis Datta, Vijay Wardhan, Kishwer Jahan
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Patent number: 9024113Abstract: Recombinant constructs and methods useful for improvement of plants are provided. In particular, recombinant constructs comprising promoters functional in plant cells positioned for expression of polynucleotides encoding starch branching enzymes from microbial sources are provided. The disclosed constructs and methods find use in production of transgenic plants to provide plants, particularly crop plants, having improved yield.Type: GrantFiled: October 29, 2007Date of Patent: May 5, 2015Assignee: Monsanto Technology LLCInventors: Yongwei Cao, Gregory J. Hinkle, Steven C. Slater, Xianfeng Chen, Barry S. Goldman
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Publication number: 20150121568Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for an Arabidopsis thaliana (AT SVL3) promoter. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.Type: ApplicationFiled: January 15, 2015Publication date: April 30, 2015Inventors: Mark Alan Chamberlin, Shai Joshua Lawitt
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Publication number: 20150113679Abstract: The present invention provides methods for obtaining plants that exhibit useful traits by perturbation of plastid function in plant rootstocks and grafting the rootstocks to scions. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants that exhibit the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that provide for plastid perturbation are also provided.Type: ApplicationFiled: September 24, 2014Publication date: April 23, 2015Inventors: Sally Mackenzie, Michael Fromm, Kamaldeep Virdi, Yashitola Wamboldt
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Patent number: 9012736Abstract: The present invention provides a method for producing plants with a desired phenotypic trait which comprises subjecting plants to mutagenesis, screening chimeric progeny for plants having the desired phenotypic trait, and propagating the survivors. In an embodiment, the phenotypic trait comprises an altered amino acid content. Preferably, the technique is used to generate Nicotiana tobacum plant lines having an increase in at least one amino acid. In a preferred embodiment, the invention provides improved Nicotiana tobacum plant lines producing at least 1.35 nmole of threonine per milligram of dry plant weight. These plants are useful for improving the flavor and aroma of the tobacco.Type: GrantFiled: July 26, 2010Date of Patent: April 21, 2015Assignee: Reynolds Technologies, Inc.Inventors: Luis Mayan Dominguez, Jerry Wayne Lawson, Wennuan Liu, Michael Francis Dube
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Publication number: 20150106977Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, and/or 3?-UTRs, isolated from Zea mays.Type: ApplicationFiled: October 15, 2014Publication date: April 16, 2015Inventors: Manju GUPTA, Navin ELANGO, Karthik N. MUTHURAMAN, Jeffrey BERINGER, Sara BENNETT, Huixia WU, Shavell GORMAN, Andrew WORDEN
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Publication number: 20150106971Abstract: There is disclosed a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a threonine synthase and having at least 90% sequence identity to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 or at least 87% sequence identity to SEQ ID NO:4, or SEQ ID NO:5; (ii) a polypeptide encoded by any one of said polynucleotides set forth in (i); or (iii) a polypeptide having at least 95% sequence identity to SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8; or (iv) a construct, vector or expression vector comprising the polynucleotide as set forth in (i).Type: ApplicationFiled: August 31, 2012Publication date: April 16, 2015Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Lucien Bovet, Nicolas Sierro
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Publication number: 20150106978Abstract: Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5?-UTRs, introns, and/or 3?-UTRs, isolated from Zea mays.Type: ApplicationFiled: October 15, 2014Publication date: April 16, 2015Inventors: Manju GUPTA, Navin ELANGO, Karthik N. MUTHURAMAN, Jeffrey BERINGER, Sara BENNETT, Huixia WU
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Publication number: 20150101078Abstract: Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.Type: ApplicationFiled: December 15, 2014Publication date: April 9, 2015Inventors: MARC C ALBERTSEN, Yukoh Hiei, Teruyuki Imayama, Masakazu Kashihara, Norio Kato, Toshihiko Komari, Toshiyuki Komori, Stanley Luck, Ichiro Oka, Hajime Sakai, Yoshimitsu Takakura, Scott V Tingey, Satoru Usami
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Publication number: 20150096069Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, enhanced nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.Type: ApplicationFiled: March 6, 2013Publication date: April 2, 2015Inventors: Paolo Castiglioni, Jaishree M. Chittoor-Vijayanath, Barry S. Goldman, Robert J. Meister, Monnanda S. Rajani, G. Ramamohan, Naveen Silvester, Tyamagondlu V. Venkatesh, Jingrui Wu, Xiaoyun Wu
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Publication number: 20150096083Abstract: The present invention provides non-coding regulatory element polynucleotide molecules isolated from the lipid transfer protein (LTP) gene of Oryza sativa and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Oryza sativa regulatory polynucleotide sequences, and methods for preparing and using the same.Type: ApplicationFiled: July 28, 2014Publication date: April 2, 2015Inventors: Wei Wu, Qi Wang, James Morrell, Linda Lutfiyya, Maolong Lu, Zhaolong Li, Ping Li, Michelle Lacy, David Kovalic, Yongwei Cao, Andrey Boukharov, Stanislaw Flasinski, Peter T. Hajdukiewicz
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Publication number: 20150089683Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.Type: ApplicationFiled: March 6, 2013Publication date: March 26, 2015Inventors: Mark Scott Abad, Pranesh Badami, Amarjit Basra, Erin Bell, Christopher P. Bonin, Paolo Castiglioni, Eric R. Cerny, Jaishree M. Chittoor-Vijayanath, Paul Chomet, Todd Dezwaan, Stephen Duff, Michael D. Edgerton, Barry S. Goldman, Balasulojini Karunanandaa, Kempagangaiah Krishnamurthy, Ping Li, Adrian A. Lund, Llinda Lutfiyya, Thomas M. Malvar, Donald E. Nelson, Monnanda S. Rajani, Dhanalakshmi Ramachandra, Thomas G. Ruff, Sara Salvador, S. Sangeetha, Char Shobha, Jeffrey M. Staub, Virginia Ursin, Tyamagondlu V. Venkatesh, Kammaradi R. Vidya, Xiaoyun Wu, Sean Yang, Jianmin Zhao
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Publication number: 20150075548Abstract: The present invention relates to genetic constructs, which can be used in the preparation of transgenic plants. The constructs can have the ability of reducing nitrate concentration in the plant, in particular the plant's leaves, and for inducing a senescence-like phenotype. The invention extends to plant cells transformed with such constructs, and to the transgenic plants themselves. The invention also relates to methods of producing transgenic plants, and to methods of reducing nitrate content in plants. The invention also relates to harvested plant leaves, for example tobacco leaves, that have been transformed with the genetic constructs, and to various tobacco articles, such as smoking articles, comprising such harvested plant leaves.Type: ApplicationFiled: March 19, 2013Publication date: March 19, 2015Inventors: Susan Davenport, Pascaline Le Lay, Juan Pablo Sanchez Tamburinno
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Publication number: 20150080553Abstract: The invention provides methods for reducing the levels of alfa (1,3)-fucosylated N-glycans on glycoproteins produced in plants or plant cells. In addition, the invention provides alfa(1,3)-fucosyltransferase genes from Nicotiana benthamiana, and mutant N. benthamiana plants in which the levels of alfa(1,3)-fucosylated N-glycans are reduced.Type: ApplicationFiled: October 4, 2012Publication date: March 19, 2015Inventors: Koen Weterings, Gerben Van Eldik
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Publication number: 20150082494Abstract: The present invention provides for a genetically modified eukaryotic host cell comprising (a) a gene encoding a first nucleotide sugar transporter (NST) operably linked to a promoter, wherein the gene and/or the promoter is heterologous to the cell, and/or (b) a native gene encoding a second NTS is disrupted and/or a promoter of the native gene is disrupted. Such modified cells can be altered in the production of polysaccharide and/or glycopeptides. The present invention also provides for methods of making or using such modified cells.Type: ApplicationFiled: June 16, 2014Publication date: March 19, 2015Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Henrik Vibe Scheller, Carsten Rautengarten, Berit Ebert, Joshua L. Heazlewood
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Publication number: 20150075547Abstract: The present invention relates to genetic constructs, which can be used in the preparation of transgenic plants. The constructs can have the ability of reducing nitrate concentration in the plant, in particular the plant's leaves, and for inducing a senescence-like phenotype. The invention extends to plant cells transformed with such constructs, and to the transgenic plants themselves. The invention also relates to methods of producing transgenic plants, and to methods of reducing nitrate content in plants. The invention also relates to harvested plant leaves, for example tobacco leaves, that have been transformed with the genetic constructs, and to various tobacco articles, such as smoking articles, comprising such harvested plant leaves.Type: ApplicationFiled: March 19, 2013Publication date: March 19, 2015Inventors: Pascaline LE LAY, Juan-Pablo SANCHEZ TAMBURRINO, Susan DAVENPORT
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Patent number: 8975471Abstract: The invention provides methods and compositions useful in target sequence suppression, target sequence validation and target sequence down regulation. The invention provides polynucleotide constructs useful for gene silencing or RNA down regulation, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence or to down regulate RNA.Type: GrantFiled: October 12, 2005Date of Patent: March 10, 2015Assignee: The Rockefeller UniversityInventors: Jose L. Reyes Taboada, Xiuren Zhang, Takashi Soyano, Nam-Hai Chua, Qi-Wen Niu, Shih-Shun Lin
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Publication number: 20150067919Abstract: The present invention relates to a genetically modified plant having an increased amount of oil in its green biomass as compared to the oil in the green biomass of its non-genetically modified counterpart. The plants may be used for producing bio-fuels such as biodiesel fuel.Type: ApplicationFiled: August 5, 2014Publication date: March 5, 2015Inventors: Vyacheslav ANDRIANOV, Mykola BORSYUK
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Publication number: 20150059010Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell. Breeding methods and methods for selecting plants utilizing a two component RNA polynucleotide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: August 20, 2014Publication date: February 26, 2015Inventors: Andrew Mark Cigan, Phillip A. Patten, Joshua K. Young
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Publication number: 20150047070Abstract: A method of producing improved drought tolerance in a plant by introducing into a plant or plant part a vector having a nucleic acid molecule which expresses or increases a polypeptide that confers drought tolerance compared to a plant in which no vector with the nucleic acid is introduced. The polypeptide is a NON-RACE-SPECIFIC DISEASE RESISTANCE 1 (NDR1).Type: ApplicationFiled: March 8, 2013Publication date: February 12, 2015Applicant: BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITYInventors: Brad Day, Patricia Santos, Caleb Knepper
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Publication number: 20150034110Abstract: The present invention relates to genetic constructs, which can be used in the preparation of transgenic plants. The constructs can have the ability of reducing nitrate concentration in the plant, in particular the plant's leaves. The invention extends to plant cells transformed with such constructs, and to the transgenic plants themselves. The invention also relates to methods of producing transgenic plants, and to methods of reducing nitrate content in plants. The invention also relates to harvested plant leaves, for example tobacco leaves, that have been transformed with the genetic constructs, and to various tobacco articles, such as smoking articles, comprising such harvested plant leaves.Type: ApplicationFiled: March 19, 2013Publication date: February 5, 2015Inventors: Susan Davenport, Pascaline Le Lay, Juan Pablo Sanchez Tamburrino
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Publication number: 20150040272Abstract: The present disclosure describes genetically-modified plants having enhanced tolerance to multiple abiotic stressors, such as extreme temperatures (heat or cold) and/or drought. Abiotic stress tolerance is enhanced by ectopic expression of a heterologous glutaredoxin. Abiotic stress tolerance (particularly drought) is also enhanced by inhibited function, activity, or expression of an endogenous glutaredoxin. Methods of producing such genetically-modified plants are also disclosed.Type: ApplicationFiled: August 31, 2012Publication date: February 5, 2015Applicants: BAYLOR COLLEGE OF MEDICINE, KANSAS STATE UNIVERSITY RESEARCH FOUNDATIONInventors: Sunghun Park, Frank White, Jungeun Park, Kendal Hirschi, Ning-Hui Cheng
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Patent number: 8940962Abstract: The present invention provides novel promoters for use in plants. Specifically, the present invention provides novel chimeric promoters comprising combinations of plant viral enhancer elements and plant promoters. The present invention also provides DNA constructs; transgenic cells, plants, and seeds containing these novel chimeric promoters; and methods for preparing and using the same.Type: GrantFiled: September 21, 2009Date of Patent: January 27, 2015Assignee: Monsanto Technology LLCInventor: Stanislaw Flasinski
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Patent number: 8940963Abstract: Process of producing in a plant, in plant tissue, or in plant cells a hetero-oligomeric protein comprising at least a first and a second protein subunit, said process comprising expressing in plant cells at least said first and said second protein subunit by (i) providing to said plant, said plant tissue or said plant cells a plus-sense single-stranded RNA viral vector encoding at least said first and said second protein subunit or (ii) providing to said plant, said plant tissue or said plant cells a first and a second plus-sense single-stranded RNA viral vector, said first viral vector encoding at least said first protein subunit, said second viral vector encoding at least said second protein subunit, whereby at least said first viral vector and said second viral vector are non-competing viral vectors.Type: GrantFiled: January 27, 2006Date of Patent: January 27, 2015Assignee: Icon Genetics GmbHInventors: Anatoly Giritch, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
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Publication number: 20150026845Abstract: Compositions and methods for modifying the production levels of alkaloids in plants are provided. Alkaloid production can be genetically controlled by modulating the transcriptional activation of PMT genes mediated by members of the ERF family and/or Myc family of transcription factors. Novel nucleotide sequences encoding the Myc family of transciption factors are also provided.Type: ApplicationFiled: June 23, 2014Publication date: January 22, 2015Inventors: Michael Paul Timko, Paul J. Rushton, Sheng-Cheng Han, Hongbo Zhang, Marta Tatiana Bokowiec
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Publication number: 20150026840Abstract: To produce a bacterial microcompartment shell, or a designed shell based on naturally occurring bacterial microcompartment shells in a new host organism, a synthetic operon is constructed that contains the desired shell protein genes and translation efficiency is controlled by host specific ribosomal binding sites. Proteins or other molecules can be encapsulated in the microcompartment shells by various methods described herein. The constructs can also be used to express self-assembling sheets comprised of shell proteins.Type: ApplicationFiled: March 14, 2014Publication date: January 22, 2015Applicant: The Regents of the University of CaliforniaInventors: Cheryl A. Kerfeld, Jonathan K. Lassila, James N. Kinney
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Publication number: 20150026841Abstract: The present invention relates to an isolated COP1 nucleic acid sequence from a maize plant and the isolated COP1 nucleic acid sequence is named as ZmCOP1. The present invention also relates to a method of using the ZmCOP1 nucleic acid sequence to control the shade avoidance response of a crop plant for high density farming and yield enhancement.Type: ApplicationFiled: June 25, 2014Publication date: January 22, 2015Inventors: Nordine Cheikh, Molian Deng, Philip W. Miller, Nanfei Xu
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Publication number: 20150013706Abstract: Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.Type: ApplicationFiled: July 28, 2014Publication date: January 15, 2015Inventors: Takashi HASHIMOTO, Akira Kato
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Publication number: 20150013705Abstract: Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.Type: ApplicationFiled: July 23, 2014Publication date: January 15, 2015Inventors: Takashi HASHIMOTO, Akira Kato
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Publication number: 20150020241Abstract: The present invention provides non-coding regulatory element polynucleotide molecules isolated from the S-adenosyl methionine synthetase (SAMS3) gene of Arabidopsis thaliana and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Arabidopsis thaliana regulatory polynucleotide sequences, and methods for preparing and using the same.Type: ApplicationFiled: June 6, 2014Publication date: January 15, 2015Inventors: Wei Wu, Qi Wang, Michael Edgerton, Kim Beazley, Jill Deikman, Michael W. Petersen, Bei Zhang
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Patent number: 8933297Abstract: The invention provides Nicotiana benthamiana mutant plants which are incapable of forming xylosyl-structures on glycoproteins. In addition, the invention provides methods for the production of heterologous glycoproteins in said mutant plants.Type: GrantFiled: June 10, 2010Date of Patent: January 13, 2015Assignee: Icon Genetics GmbHInventors: Koen Weterings, Gerben Van Eldik
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Publication number: 20150013033Abstract: The present invention provides an expression cassette containing a polynucleotide coding sequence for a hydroxycinnamoyl-CoA hydratase-lyase (HCHL), which is operably linked to a heterologous promoter. Also provided are methods of engineering plants that have reduced lignification, as well as cells, plant parts, and plant tissues from such engineered plants.Type: ApplicationFiled: July 13, 2012Publication date: January 8, 2015Applicant: The Regents of the University of CaliforniaInventors: Dominique Loqué, Amyerick Guillaume Eudes