Abstract: The relationship between F-box proteins and proteins involved in the ethylene response in plants is described. In particular, F-box proteins may bind to proteins involved in the ethylene response and target them for degradation by the ubiquitin/proteasome pathway. The transcription factor EIN3 is a key transcription factor mediating ethylene-regulated gene expression and morphological responses. EIN3 is degraded through a ubiquitin/proteasome pathway mediated by F-box proteins EBF1 and EBF2. The link between F-box proteins and the ethylene response is a key step in modulating or regulating the response of a plant to ethylene. Described herein are transgenic plants having an altered sensitivity to ethylene, and methods for making transgenic plant having an altered sensitivity to ethylene by modulating the level of activity of F-box proteins. Methods of altering the ethylene response in a plant by modulating the activity or expression of an F-box protein are described.

Abstract: The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.

Abstract: The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic isocitrate dehydrogenase (icdh) gene that modulates nitrogen use in plants. The invention also relates to stacking the icdh gene with other exogenous or heterologous genes that modulate nitrogen use in the plant, including a N-acetylglutamate kinase gene. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.

Abstract: Provided herein are, inter alia, transgenic plants with altered ethylene sensitivity. The transgenic plants provided herein express an EIN2 protein including an amino acid mutation at a position corresponding to position 645 of SEQ ID NO:1. Expression of the EIN2 protein carrying the mutation at position 645 will result in plants with modulated ethylene sensitivity. In some embodiments, the mutation at position 645 of the EIN2 protein will result in plants with increased ethylene sensitivity. Alternatively, in other embodiments, the mutation at position 645 of the EIN2 protein will result in plants with decreased ethylene sensitivity.

Abstract: The invention relates to transgenic plants exhibiting enhanced growth rates, seed and fruit yields, and overall biomass yields, as well as methods for generating growth-enhanced transgenic plants. In one embodiment, transgenic plants engineered to over-express glutamine phenylpyruvate transaminase (GPT) are provided.

Abstract: This invention provides genetically modified photosynthetic organisms and methods and constructs for enhancing inorganic carbon fixation. A photosynthetic organism of the present invention comprises a RUBISCO fusion protein operatively coupled to a protein-protein interaction domain to enable the functional association of RUBISCO and carbonic anhydrase.

Abstract: The present invention relates to genetically modified plants expressing altered inflorescence. More particularly, expression of at least two flavonoid 3?S? hydroxylases and at least one dihydroflavonol-4-reductase is achieved, leading to the production of dephinidin pigments and altered color phenotypes.

Abstract: A rose is produced in which an introduced gene is only present in a part of the cells thereof, such as cells of the L1 layer of flower petals, but is not present in germ cells such as pollen cells or ovule cells. Since the introduced gene is not propagated to other roses even when this rose is crossed with other roses, the possibility of dispersal of the introduced gene can be completely negated.

Abstract: The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.

Abstract: The promoter of a soybean lipid transfer protein LTP4 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

Abstract: The invention relates to genetically engineered plants with altered inflorescence. Plants such as spray carnations are transformed with a non-indigenous flavonoid 3?,5? hydroxylase (F3?5?H) and dihydroflavanol-4-reductase (DFR) in conjunction with a genetic suppressor of indigenous DFR. Preferably the substrate specificity of the indigenous DFR is different to the non-indigenous DFR in order to enhance the colour of the inflorescence.

Abstract: The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine-insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants.

Abstract: The relationship between F-box proteins and proteins involved in the ethylene response in plants is described. In particular, F-box proteins may bind to proteins involved in the ethylene response and target them for degradation by the ubiquitin/proteasome pathway. The transcription factor EIN3 is a key transcription factor mediating ethylene-regulated gene expression and morphological responses. EIN3 is degraded through a ubiquitin/proteasome pathway mediated by F-box proteins EBF1 and EBF2. The link between F-box proteins and the ethylene response is a key step in modulating or regulating the response of a plant to ethylene. Described herein are transgenic plants having an altered sensitivity to ethylene, and methods for making transgenic plant having an altered sensitivity to ethylene by modulating the level of activity of F-box proteins. Methods of altering the ethylene response in a plant by modulating the activity or expression of an F-box protein are described.

Abstract: The invention provides a method for producing a flowering plant which can flower at a low light intensity. Specifically, the invention relates to a transgenic plant which can flower in a low light environment; a method for producing the same; a method for inducing the same to flower; and so on. More specifically, the invention relates to a transgenic plant which is engineered so as to flower in a low light environment (e.g., indoors) by introducing a FT gene thereinto; a method for producing the same; a method for inducing the same to flower; and so on.

Abstract: A rose is produced in which an introduced gene is only present in a part of the cells thereof, such as cells of the L1 layer of flower petals, but is not present in germ cells such as pollen cells or ovule cells. Since the introduced gene is not propagated to other roses even when this rose is crossed with other roses, the possibility of dispersal of the introduced gene can be completely negated.

Abstract: The present invention relates to an isolated nucleic acid molecule, comprising a nucleotide sequence, which encodes a polypeptide with chalcone 3-hydroxylase activity, wherein the nucleotide sequence comprises SEQ ID NO. 1 or has at least a 60% identity with SEQ ID NO. 1 or is able to hybridize with a molecule comprising the sequence of SEQ ID NO. 1, wherein the nucleotide sequence encodes a polypeptide, which comprises the motif FASRPLSX1X2G(X3)m(GSAGGD)n (SEQ ID NO. 3), wherein X1 is threonine or serine, X2 is alanine or glycine, X3 is any amino acid, m is an integer between 50 and 200, and n is 0 or 1.

Abstract: The promoters of a soybean SC194 polypeptide and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

Abstract: The invention relates to transgenic plants exhibiting dramatically enhanced growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, more efficient nitrogen utilization, increased tolerance to high salt conditions, and increased biomass yields. In one embodiment, transgenic plants engineered to over-express both glutamine phenylpyruvate transaminase (GPT) and glutamine synthetase (GS) are provided. The GPT+GS double-transgenic plants of the invention consistently exhibit enhanced growth characteristics, with T0 generation lines showing an increase in biomass over wild type counterparts of between 50% and 300%. Generations that result from sexual crosses and/or selfing typically perform even better, with some of the double-transgenic plants achieving an astounding four-fold biomass increase over wild type plants.

Abstract: The present invention relates to genetically modified plants expressing altered inflorescence. More particularly, expression of at least two flavonoid 3?S? hydroxylases and at least one dihydroflavonol-4-reductase is achieved, leading to the production of dephinidin pigments and altered colour phenotypes.

Abstract: The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct includes one or more nucleic acid sequences selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.

Abstract: The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3?,5?-hydroxylase (F3?5?H) activity and to the use of the genetic sequence and/or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3?5?H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3?5?H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

Abstract: The present invention relates to a method for enhancement of photosynthesis and biomass of a plant by plastid transformation with MDH gene, more precisely a method for enhancement of photosynthesis and biomass of C3 plant by plastid transformation system with MDH gene. The MDH plastid transgenic plant prepared by the method of the present invention exhibits not only increased photosynthesis efficiency but also increased growth rate, leaf area, stem diameter and biomass of the plant, compared with the control plant. Therefore, the plastid transformed C3 plant prepared by C4 type gene introduction can be effectively used for enhancing photosynthesis and biomass of the plant.

Abstract: Methods and materials for modulating lysine, glucose, fructose, galactose and leucine content in plants are disclosed.

Abstract: Disclosed are novel genetically modified plant cells wherein a SHI (short internodes) family gene is integrated into the nuclear genome. Also disclosed are plant cells where a SHI antisense gene is integrated or plants including heterologous expression control of autologous SHI genes. The plant cells confer novel phenotypes upon plants incorporating the SHI family gene. The invention also discloses transgenic plants and methods for plant production, where the plants are dwarfed, but exhibit normal or increased flower set after induction of flowering with GA. The plants of the invention are obtained without use of any growth retardants.

Abstract: The present invention discloses transgenic plants having an altered level of NAP protein compared to that of a non-transgenic plant, where the transgenic plants display an altered leaf senescence phenotype relative to a non-transgenic plant, as well as mutant plants comprising an inactivated NAP gene, where mutant plants display a delayed leaf senescence phenotype compared to that of a non-mutant plant. The present invention also discloses methods for delaying leaf senescence in a plant, as well as methods of making a mutant plant having a decreased level of NAP protein compared to that of a non-mutant plant, where the mutant plant displays a delayed leaf senescence phenotype relative to a non-mutant plant. Methods for causing precocious leaf senescence or promoting leaf senescence in a plant are also disclosed. Also disclosed are methods of identifying a candidate plant suitable for breeding that displays a delayed leaf senescence and/or enhanced yield phenotype.

Abstract: A method of modifying morphology in a plant by introducing into a plant at least one chimaeric gene having a promoter sequence operably associated with a nucleic acid sequence, the promoter sequence being operable to direct expression in specific cells of the plant and the nucleic acid sequence encoding at least one gene product capable of altering the metabolism of or causing death of the specific cells and/or nearby cells. In particular, the promoter sequence is operable to direct expression in lateral bud or lateral shoot and the nucleic acid encoding at least one gene product capable of disrupting the metabolism of or causing the death of the lateral bud or lateral shoot or nearby cells. Preferably the promoter sequence has the sequence shown as SEQ ID No. 1 or SEQ ID No. 7 or SEQ ID No. 4, or a part thereof capable of regulating expression of a gene, or a sequence having at least 60%, preferably at least 75%, homology to SEQ ID No. 1 or SEQ ID No. 7 and being capable of regulating expression of a gene.

Abstract: Disclosed are proteins, and nucleic acids encoding such proteins, involved in or associated with cell proliferation, senesence, differentiation, development, and stress response in plants. Also disclosed are uses for such proteins.

Abstract: Disclosed are proteins, and nucleic acids encoding such proteins, involved in or associated with the stress response (both biotic and abiotic stress) in plants. Also disclosed are uses for such proteins.

Abstract: The promoters of a soybean lipid transfer protein LTP1 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

Abstract: The present invention relates to a plastid transformation system capable of preventing second recombination events of heterologous genes inserted into plastid genomes. More specifically, this invention relates to a plastid transformation vector carrying a promoter and a terminator derived from organisms other than tobacco. The inventive recombinant expression vector for plastid transformation is capable of mass-producing exogenous proteins on a level par with conventional vectors carrying promoter/terminator couples of tobacco origin. At the same time, it is capable of preventing second recombination events within plastids. Thus, the vector of this invention is greatly useful in producing transgenic plants since it can effect a secure introduction of heterologous genes and support normal transformation and heterologous gene expression.

Abstract: The promoters of a soybean lipid transfer protein LTP2 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

Abstract: The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.

Abstract: Regulation of expression of programmed cell death, including senescence, in plants is achieved by integration of a gene or gene fragment encoding senescence-induced deoxyhypusine synthase, senescence-induced elF-5A or both into the plant genome in antisense orientation. Plant genes encoding senescence-induced deoxyhypusine synthase and senescence-induced elF-5A are identified and the nucleotide sequences of each, alone and in combination are used to modify senescence in transgenic plants.

Abstract: A method of transforming a carnation (Dianthus L.) plant genome with a DNA molecule. The method comprises (a) preparing stem explants from carnation cuttings; (b) wounding the explants by microprojectile bombardment; (c) cocultivating the wounded explants with Agrobacterium comprising the DNA molecule under defined conditions of exposure to dark followed by light; (d) excising shoots from the cultivated wounded explants and removing the leaves from the shoots; and (e) culturing the leaves to obtain transgenic shoots transformed with the DNA molecule. Also disclosed are a rolC-transgenic carnation with improved agronomic traits and enhancement of flower fragrance by antisense suppression of the flavonoid gene fht.

Abstract: The present invention relates to the isolation and identification of a nucleic acid molecule which regulates fruit size and/or cell division in plants and the protein encoded by such a nucleic acid molecule. The invention also relates to an expression vector containing the encoding nucleic acid and methods whereby fruit size is reduced and/or increased and cell division is regulated by transformation of plants with the disclosed nucleic acid molecule. Host cells as well as transgenic plants and plant seeds containing the nucleic acid molecule of the present invention are also discussed.

Abstract: The present invention relates to a novel interspecific Dianthus plant. The Dianthus plant of the present invention was developed through a unique interspecific cross between Dianthus caespitosus and Dianthus barbatus.

Abstract: The field of the invention is inducible gene expression systems wherein expression is controlled by a novel ecdysone receptor or its derivatives. The field particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel ecdysone receptor. The nucleic acid and polypeptides are useful in novel gene expression systems inducible with ecdysone or derivative receptor agonists.

Abstract: The present invention provides, inter alia, nucleic acids which encode P450s in corn that, when expressed in the presence of a reductase, metabolize compounds exemplary of several distinct classes of insecticides and herbicides. The invention also includes amino acids encoded by the nucleic acids, as well as vectors, cells and eukaryotes comprising the nucleic or amino acid compounds. Also included are methods using the materials provided.

Abstract: The present invention is directed to an isolated protein or polypeptide which elicits a hypersensitive response in plants as well as an isolated DNA molecule which encodes the hypersensitive response eliciting protein or polypeptide. This isolated protein or polypeptide and the isolated DNA molecule can used to impart disease resistance to plants, to enhance plant growth, and/or to control insects on plants. This can be achieved by applying the hypersensitive response elicitor protein or polypeptide in a non-infectious form to plants or plant seeds under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds.