Search Patents
  • Multiplexed luciferase reporter assay systems
    Patent number: 8569002
    Abstract: Inhibitors of luciferase enzymes are disclosed and find use in multiplexed assays using multiple luciferases and multiple inhibitors, in both in vitro and in vivo embodiments.
    Type: Grant
    Filed: October 20, 2010
    Date of Patent: October 29, 2013
    Assignee: The Board of Regents of the University of Texas System
    Inventors: Lawrence Lum, Ozlem Kulak
  • Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
    Patent number: 11293047
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Grant
    Filed: December 23, 2019
    Date of Patent: April 5, 2022
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • Luciferase Detection Assay System
    Publication number: 20090047693
    Abstract: The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
    Type: Application
    Filed: September 12, 2008
    Publication date: February 19, 2009
    Applicant: PerkinElmer Life and Analytical Sciences B.V.
    Inventors: Harry van Lune, Johan Jochem Bruggeman
  • Luciferase Detection Assay System
    Publication number: 20090053744
    Abstract: The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
    Type: Application
    Filed: April 8, 2008
    Publication date: February 26, 2009
    Applicant: PerkinElmer Life and Analytical Sciences B.V.
    Inventors: Harry van Lune, Johan Jochem Bruggeman
  • luciferase variant for high-throughput screening
    Patent number: 8980603
    Abstract: Described herein is a variant of wild type Gaussia luciferase that catalyzes glow-type emission kinetics suited for high-throughput functional screening applications. Polypeptides, functional fragments, variants, and nucleic acids that encode the enhanced luciferase are further described. One such polypeptide corresponds to wild type Gaussia luciferase with a substitution mutation of I for M at position 43 of the mature peptide. Methods of use, assay systems and kits that contain the polypeptides and/or nucleic acids are further described.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: March 17, 2015
    Assignee: The General Hospital Corporation
    Inventors: Bakhos A. Tannous, Casey Maguire
  • Luciferase assay, compositions and kits for use in connection therewith
    Patent number: 6183978
    Abstract: An assay for the presence of luciferase in a biological sample offers heightened sensitivity, signal intensity and persistence. The assay is sensitive down to 50 fg luciferase. The biological sample is combined with essential ingredients luciferin, ADP, myokinase and Mg++. The myokinase converts ADP to ATP, necessary for the luciferase reaction, and AMP, which retards the reaction kinetics. The resulting assay exhibits a persistent glow emission which makes it adaptable to automation.
    Type: Grant
    Filed: September 4, 1998
    Date of Patent: February 6, 2001
    Assignee: Tropix, Inc.
    Inventors: Irena Bronstein, Corinne E. M. Olesen, John C. Voyta, Yu-Xin Yan
  • Luciferase-based assays
    Patent number: 7741067
    Abstract: A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Grant
    Filed: December 23, 2003
    Date of Patent: June 22, 2010
    Assignee: Promega Corporation
    Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
  • Modified renilla luciferase nucleic acids and methods of use
    Publication number: 20120035077
    Abstract: This invention provides modified nucleotide sequences encoding luciferase that have greater expression than wild type luciferase.
    Type: Application
    Filed: May 27, 2011
    Publication date: February 9, 2012
    Applicant: SwitchGear Genomics
    Inventors: Nathan D. Trinklein, Shelley Force Aldred
  • Method for enhancing activity of luciferase with fluorescence activity
    Patent number: 7396655
    Abstract: Methods for enhancing luminescence of a luciferase (BFP-aq) with fluorescence activity derived from a calcium-binding photoprotein are provided. To a luciferase solution with fluorescence activity that contains an apoprotein, a calcium-binding photoprotein, which is constituted such that a coelenteramide or an analog thereof is coordinated inside, a coelenterazine that is the luminescent substrate of the luciferase or an analog thereof and a compound (e.g., imidazole etc.) having the function of removing an —NH-proton of the pyrazine ring of the imidazopyrazine skeleton in the coelenterazine or the analog thereof are added.
    Type: Grant
    Filed: March 30, 2006
    Date of Patent: July 8, 2008
    Assignee: Chisso Corporation
    Inventors: Satoshi Inouye, Satoko Sasaki
  • Luciferase detection reagent kits and luciferase detection method using the kit
    Patent number: 6852499
    Abstract: The present invention provides a detection method and a method of manufacturing a detection kit, both characterized by use of an organic sulfur reagent, and which are effective at low concentration of the reagent, are inexpensive, and have reduced unpleasent odor. Provided is a reagent kit for detecting a Coleoptera luciferase, comprising an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion. Also provide is a method for detecting a Coleoptera luciferase, comprising step 1 of mixing an aqueous solution, containing an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion, with a sample containing a Coleoptera luciferase, to give a mixed solution; and step 2 of measuring the light emitted in the mixed solution.
    Type: Grant
    Filed: March 13, 2003
    Date of Patent: February 8, 2005
    Assignees: Toyo B-Net Co., Ltd.
    Inventors: Masayuki Ryufuku, Hozumi Tanaka, Chie Suzuki
  • Luciferase and photoprotein
    Patent number: 7125697
    Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector. Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein. These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.
    Type: Grant
    Filed: March 4, 2003
    Date of Patent: October 24, 2006
    Assignee: Chisso Corporation
    Inventor: Satoshi Inouye
  • Nucleic acid molecules encoding red and green emitting luciferases
    Publication number: 20050089964
    Abstract: Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.
    Type: Application
    Filed: September 14, 2004
    Publication date: April 28, 2005
    Inventors: Vadim Viviani, Yoshihiro Ohmiya
  • Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
    Patent number: 6232107
    Abstract: Isolated and purified nucleic acid molecules that encode a luciferase from Renilla mulleri, Gaussia and Pleuromamma, and the proteins encoded thereby are provided. Isolated and purified nucleic acids encoding green fluorescent proteins from the genus Renilla and Ptilosarcus, and the green fluorescent proteins encoded thereby are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.
    Type: Grant
    Filed: March 26, 1999
    Date of Patent: May 15, 2001
    Assignees: Prolume, LTD
    Inventors: Bruce J. Bryan, Christopher Szent-Gyorgyi
  • GAUSSIA LUCIFERASE VARIANT FOR HIGH-THROUGHPUT SCREENING
    Publication number: 20120122182
    Abstract: Described herein is a variant of wild type Gaussia luciferase that catalyzes glow-type emission kinetics suited for high-throughput functional screening applications. Polypeptides, functional fragments, variants, and nucleic acids that encode the enhanced luciferase are further described. One such polypeptide corresponds to wild type Gaussia luciferase with a substitution mutation of I for M at position 43 of the mature peptide. Methods of use, assay systems and kits that contain the polypeptides and/or nucleic acids are further described.
    Type: Application
    Filed: June 30, 2010
    Publication date: May 17, 2012
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Bakhos A. Tannous, Casey Maguire
  • METHOD AND AGENTS FOR DETECTING LUCIFERASE ACTIVITY
    Publication number: 20180163248
    Abstract: The invention provides methods and reagents for detecting luciferase in biological samples. The methods and reagents of the present invention allow detecting fungal luciferase or a functional analog thereof.
    Type: Application
    Filed: April 21, 2016
    Publication date: June 14, 2018
    Inventors: Il'ya Viktorovich Yampol'skiy, Valentin Nikolaevich PETUSHKOV, Konstantin Viktorovich PURTOV, Natal'ya Sergeevna RODIONOVA, Mikhail Sergeevich BARANOV
  • METHOD AND AGENTS FOR DETECTING LUCIFERASE ACTIVITY
    Publication number: 20240018566
    Abstract: The invention provides methods and reagents for detecting luciferase in biological samples. The methods and reagents of the present invention allow detecting fungal luciferase or a functional analog thereof.
    Type: Application
    Filed: September 27, 2023
    Publication date: January 18, 2024
    Inventors: ll'ya Viktorovich YAMPOL'SKIY, Valentin Nikolaevich PETUSHKOV, Konstantin Viktorovich PURTOV, Natal'ya Sergeevna RODIONOVA, Mikhail Sergeevich BARANOV
  • Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
    Patent number: 6436682
    Abstract: Isolated and purified nucleic acid molecules that encode a luciferase from Renilla mulleri, Gaussia and Pleuromamma, and the proteins encoded thereby are provided. Isolated and purified nucleic acids encoding green fluorescent proteins from the genus Renilla and Ptilosarcus, and the green fluorescent proteins encoded thereby are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.
    Type: Grant
    Filed: June 30, 2000
    Date of Patent: August 20, 2002
    Assignee: Prolume, Ltd.
    Inventors: Bruce J. Bryan, Christopher Szent-Gyorgyi
  • Kits and reagents for luciferase detection assay system
    Patent number: 7951551
    Abstract: The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
    Type: Grant
    Filed: April 8, 2008
    Date of Patent: May 31, 2011
    Assignee: PerkinElmer Life and Analytical Sciences B.V.
    Inventors: Harry van Lune, Johan Jochem Bruggeman
  • Luciferase and photoprotein
    Publication number: 20040002127
    Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector.
    Type: Application
    Filed: March 4, 2003
    Publication date: January 1, 2004
    Applicant: CHISSO CORPORATION
    Inventor: Satoshi Inouye
  • METHOD AND AGENTS FOR DETECTING LUCIFERASE ACTIVITY
    Publication number: 20200172952
    Abstract: The invention provides methods and reagents for detecting luciferase in biological samples. The methods and reagents of the present invention allow detecting fungal luciferase or a functional analog thereof.
    Type: Application
    Filed: January 27, 2020
    Publication date: June 4, 2020
    Applicant: OBSCHESTVO S OGRANICHENNOY OTVETSTVENNOSTYU "PLANTA"
    Inventors: Il'ya Viktorovich YAMPOL'SKIY, Valentin Nikolaevich PETUSHKOV, Konstantin Viktorovich PURTOV, Natal'ya Sergeevna RODIONOVA, Mikhail Sergeevich BARANOVA
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