Search Patents
  • Human G-protein coupled receptors
    Patent number: 6060272
    Abstract: Two human G-protein coupled receptor polypeptides and DNA (RNA) encoding each of such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides. Also disclosed are diagnostic methods for detecting a mutation in the nucleic acid sequence of each of the G-protein coupled receptors.
    Type: Grant
    Filed: May 7, 1997
    Date of Patent: May 9, 2000
    Assignee: Human Genome Sciences, Inc.
    Inventors: Yi Li, Steven M. Ruben
  • METHODS AND COMPOSITIONS FOR TARGETED CLEAVAGE AND RECOMBINATION
    Publication number: 20140369980
    Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
    Type: Application
    Filed: July 31, 2014
    Publication date: December 18, 2014
    Inventors: Michael C. Holmes, Fyodor Urnov
  • Nucleic acid encoding chimeric complement inhibitor proteins
    Patent number: 5624837
    Abstract: Chimeric complement inhibitor proteins are provided which include a first functional domain (first amino acid sequence) having C3 inhibitory activity and a second functional domain (second amino acid sequence) having C5b-9 inhibitory activity. The first functional domain is amino terminal to the second functional domain. In this way, the chimeric protein exhibits both C3 and C5b-9 inhibitory activity. The other orientation, i.e., the orientation in which the second amino acid sequence is amino terminal to the first amino acid sequence, only produces C3 inhibitory activity. Nucleic acid molecules encoding such proteins are also provided.
    Type: Grant
    Filed: June 1, 1995
    Date of Patent: April 29, 1997
    Assignee: Alexion Pharmaceuticals, Inc.
    Inventors: William L. Fodor, Scott Rollins, Stephen P. Squinto
  • Chimeric HCV/GBV-B viruses
    Publication number: 20010034019
    Abstract: This invention relates to nucleic acids encoding HCV/GBV-B constructs and chimeric, infectious HCV/GBV-B viruses that comprise a non-structural (NS) protein function of hepatitis C virus (HCV). The invention further relates to methods for using the nucleic acid constructs and chimeric, infectious viruses to screen for HCV inhibitors in cell culture models or in animal models of viral infection.
    Type: Application
    Filed: December 21, 2000
    Publication date: October 25, 2001
    Inventors: Zhi Hong, Nancy J. Butkiewicz, Weidong Zhong, Paul Ingravallo, Jacquelyn Wright-Minogue, Johnson YN Lau, Stanley M. Lemon
  • RECOMBINANT SEGMENTED NEGATIVE STRAND VIRUS CONTAINING BICISTRONIC VRNA SEGMENT WITH A DUPLICATION OF ITS 3? NONCODING FLANKING SEQUENCE, AND VACCINES AND THERAPEUTIC COMPOSITIONS CONTAINING THE SAME
    Patent number: 6723559
    Abstract: The present invention relates to recombinant negative strand RNA molecules which may be used to express heterologous proteins in animal cells and/or to construct recombinant viruses able to express heterologous proteins during their multiplication in host animal cells.
    Type: Grant
    Filed: April 17, 2002
    Date of Patent: April 20, 2004
    Assignee: Institute Pasteur
    Inventors: Nicolas Robert Xavier Escriou, Sylvie Van Der Werf, Alexandre Vieira-Machado, Nadia Naffakh
  • Leafhopper ecdysone receptor nucleic acids, polypeptides, and uses thereof
    Patent number: 8021878
    Abstract: The present invention relates to a novel isolated leafhopper ecdysone receptor polypeptide. The invention also relates to an isolated nucleic acid encoding the leafhopper ecdysone receptor polypeptide, to vectors comprising them and to their uses, in particular in methods for modulating gene expression in an ecdysone receptor-based gene expression modulation system and methods for identifying molecules that modulate leafhopper ecdysone receptor activity.
    Type: Grant
    Filed: August 13, 2007
    Date of Patent: September 20, 2011
    Assignee: Intrexon Corporation
    Inventor: Subba Reddy Palli
  • Method for expanding adult stem cells from blood and compositions and methods for using the same
    Patent number: 8263400
    Abstract: A method for the expansion of adult stem cells from blood, particularly but not only peripheral blood, involves removing adult stem cells from blood of a mammal, immediately expanding the stem cells via in-vitro treatment with MCSF (Macrophage Colony Stimulating Factor) at a concentration of about 8-15 nM, and purifying the expanded stem cells. Compositions and methods of using the expanded adult stem cells are also described.
    Type: Grant
    Filed: March 20, 2009
    Date of Patent: September 11, 2012
    Assignee: Thankstem Srl
    Inventors: Alessandra Gambacurta, Marco Polettini
  • Circular DNA molecule with conditional origin of replication, method for preparing the same and use thereof in gene therapy
    Patent number: 6977174
    Abstract: A circular DNA molecule, useful for gene therapy, comprising at least one nucleic acid sequence of interest, characterized in that the region allowing the replication thereof has an origin of replication with a functionality in a host cell that requires the presence of at least one specific protein foreign to said host cell. A method for preparing same, cells incorporating said DNA molecules and uses thereof in gene therapy are also described.
    Type: Grant
    Filed: September 13, 1996
    Date of Patent: December 20, 2005
    Assignee: Centelion
    Inventors: Joël Crouzet, Fabienne Soubrier
  • Novel human kunitz-type inhibitors and methods relating thereto
    Publication number: 20020098560
    Abstract: The present invention provides isolated DNA molecules comprising a DNA segment encoding novel human Kunitz-type inhibitors. Also provided are DNA constructs comprising a first DNA segment encoding a novel human Kunitz-type inhibitor wherein said first DNA segment is operably linked to additional DNA segments required for the expression for the first DNA segment, as well as host cells containing such DNA constructs and methods for producing proteins from the host cells.
    Type: Application
    Filed: July 13, 2001
    Publication date: July 25, 2002
    Applicant: ZymoGenetics, Inc.
    Inventors: Cindy A. Sprecher, Walt Kisiel, Donald C. Foster
  • Method of reducing neuronal electrical activity with a potassium channel subunit
    Patent number: 7192773
    Abstract: Methods of reducing the excitability of an excitable cell by transforming an excitable cell with a nucleic acid construct encoding an open rectifier K+ channel (dORK) (SEQ ID NO:2) or a modified open rectifier K+ channel (dORK?) (SEQ ID NO:4), and expressing the open rectifier K+ channel in the excitable cell, wherein the excitability of the transformed cell is reduced. Also featured are transgenic animals expressing dORK or dORK?.
    Type: Grant
    Filed: May 15, 2003
    Date of Patent: March 20, 2007
    Assignee: New York University
    Inventors: Michael N. Nitabach, Justin Blau, Todd C. Holmes, Steven A. N. Goldstein
  • Isolated transgenic mammalian neural cell for detection of a sample containing a chemical substance damage to neurological system or selection of drugs for treating neurodegenerative disorders
    Patent number: 8211695
    Abstract: The invention is to provide an isolated transgenic mammalian neural cell, which comprises at least one heterologous vector expressing AhR/ARNT. Also provided is methods the detection of a sample containing a chemical substance damage to the nervous system and the selection of drugs for treating neurodegenerative disorders.
    Type: Grant
    Filed: March 31, 2010
    Date of Patent: July 3, 2012
    Assignee: Taipei Medical University
    Inventors: Yi-Hsuan Lee, Cheng-Yu Wang, Shu-Hui Juan, Wen-Liang Chen, Kuo-Sheng Hung, Chun-Hua Lin
  • TDF-related compounds and analogs thereof, analogs and bioactive fragments
    Patent number: 8911953
    Abstract: The present invention relates generally to tissue differentiation factor (TDF) analogs. More specifically, the invention relates to structure-based methods and compositions useful in designing, identifying, and producing molecules, which act as functional modulators of TDF-like receptors. The invention further relates to methods of detecting, preventing, and treating TDF-associated disorders.
    Type: Grant
    Filed: September 20, 2012
    Date of Patent: December 16, 2014
    Assignee: Thrasos Innovation, Inc.
    Inventors: William D. Carlson, Peter C. Keck, Dattatreyamurty Bosukonda, Philippe Bey
  • Biofilm inhibiting composition
    Patent number: 8906393
    Abstract: Polysaccharide-containing extracts isolated from a host cell containing nucleotide sequences encoding genes pamA, pamB and pamC, wherein the extract is capable of inhibiting biofilm formation produced by gram-negative bacteria, gram-positive bacteria and fungi, and methods to inhibit biofilm formation or remove biofilms that have already formed.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: December 9, 2014
    Assignee: Rutgers, The State University of New Jersey
    Inventors: Jeffrey B. Kaplan, Nataliya V. Balashova, Scott C. Kachlany, Evguenii Vinogradov
  • Method of diminishing the symptoms of neurodegenerative disease
    Patent number: 7638119
    Abstract: A method of diminishing the symptoms of neurodegenerative disease in a patient is disclosed. In one embodiment, the method comprises the steps of: (a) identifying a patient with a neurodegenerative disease, (b) producing a cell culture, wherein the cell culture comprises cells with induced antioxidant response element (ARE) mediated transcription, and (c) transplanting at least a portion of the cell culture into the brain of the patient, wherein symptoms of neurodegenerative disease are diminished.
    Type: Grant
    Filed: November 30, 2005
    Date of Patent: December 29, 2009
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Jeffrey A. Johnson, Marcus J. Calkins
  • Androgen-repressed metastatic human prostate cancer cell line
    Patent number: 5874305
    Abstract: A new prostate cancer cell line is a continuous line of androgen-repressed metastatic prostate cancer cells. Androgen-repressed prostate cancer cells are linked with aggressive, malignant prostate cancer, in the identification and establishment of androgen-repressed prostate cancer cell lines, which are stable and continuous, allows the examination of therapeutic agents therefor, as well as identification of biological markers for the same.
    Type: Grant
    Filed: January 30, 1996
    Date of Patent: February 23, 1999
    Assignee: University of Virginia Patent Foundation
    Inventors: Leland W.K. Chung, Haiyen E. Zhau, Shi-ming Chang
  • Thermophilic endoglucanase
    Publication number: 20030129723
    Abstract: The present invention provides a polypeptide that has a thermophilic endoglucanase activity, a DNA that comprises a nucleotide sequence encoding the polypeptide, a recombinant DNA and an expression vector that comprise the DNA, a transformant that is transformed by the expression vector, and a method of producing the polypeptide using the transformant. The endoglucanase of the present invention shows a maximum activity at a high temperature range around 97° C., so that it is industrially useful.
    Type: Application
    Filed: November 14, 2002
    Publication date: July 10, 2003
    Applicant: RAKUTO KASEI INDUSTRIAL CO., LTD.
    Inventors: Kazuhiko Ishikawa, Hiroyasu Ishida, Yoshitsugu Kosugi, Susumu Ando, Akio Shimomura
  • Expanding the eukaryotic genetic code
    Patent number: 7618775
    Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
    Type: Grant
    Filed: April 16, 2004
    Date of Patent: November 17, 2009
    Assignee: The Scripps Research Institute
    Inventors: Jason W. Chin, T. Ashton Cropp, J. Christopher Anderson, Peter G. Schultz
  • ARTIFICIAL CHROMOSOME VECTOR
    Publication number: 20110229932
    Abstract: According to the present invention there is provided a method of producing a protein in a eukaryotic cell line, comprising the steps of a) providing a backbone of an artificial chromosome, b) recombining the nucleic acid encoding said protein into said backbone to generate an expression vector, c) introducing said expression vector into a eukaryotic host cell line to obtain a eukaryotic expression cell line, d) cultivating said expression cell line to produce said protein, and e) isolating said protein. The invention further relates to respective vectors and transgenic cell lines.
    Type: Application
    Filed: November 18, 2009
    Publication date: September 22, 2011
    Inventors: Anton Bauer, Emilio Manuel Casanova Hevia, Leander Blaas
  • Modulators that promote cell surface expression of odorant receptors
    Patent number: 7425445
    Abstract: The present invention relates to polypeptides capable of promoting odorant receptor cell surface localization and odorant receptor functional expression. The present invention further provides assays for the detection of ligands specific for various odorant receptors. Additionally, the present invention provides methods of screening for odorant receptor accessory protein polymorphisms and mutations associated with disease states, as well as methods of screening for therapeutic agents, ligands, and modulators of such proteins.
    Type: Grant
    Filed: June 20, 2005
    Date of Patent: September 16, 2008
    Assignee: Duke University
    Inventors: Hiroaki Matsunami, Momoka Matsunami, Harumi Saito, Hanyi Zhuang
  • Method for producing cloned bovines from cells of a bovine intramuscular preadipocyte cell line
    Patent number: 6489536
    Abstract: For stably and efficiently producing cloned livestock, used is a uniform cultured cell strain as a donor cell for nuclear transplantation.
    Type: Grant
    Filed: July 19, 2001
    Date of Patent: December 3, 2002
    Assignees: Director General of National Agricultural Research Organization, President, National Livestock Breeding Center (NLBC) Independent Administrative Institution
    Inventors: Hisashi Aso, Norikazu Miyashita, Takato Takenouchi, Kyouhei Ozutsumi, Yasuo Shioya, Tadayoshi Mitsuhashi, Norio Saito, Shuji Kobayashi, Kanako Kaneyama, Toshiyuki Kojima, Shigeru Katamine