Search Patents
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Publication number: 20020119542Abstract: Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.Type: ApplicationFiled: November 14, 2001Publication date: August 29, 2002Inventors: Vadim R. Viviani, Yoshihiro Ohmiya
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Publication number: 20090235370Abstract: The present invention generally relates to a methods, compositions and assays for real-time monitoring of the progression of a disease, such as a cancer in a subject, by measuring the level of bioluminescence signal in a biological sample obtained from a subject, where the bioluminescence signal is from a secreted luciferase protein expressed by a cell or tissue in the subject. One aspect of the present invention relates to administering to a subject a nucleic acid encoding a secreted luciferase, and in some embodiments, a disease or a diseased tissue such as tumor cells expresses the secreted luciferase protein, which is monitored in a biological sample, such as blood or urine obtained from a subject. One aspect of the invention relates to analysis of a secreted luciferase protein by measuring the level in a biological sample obtained from the subject without the need for invasive monitoring procedures.Type: ApplicationFiled: January 16, 2009Publication date: September 17, 2009Applicant: THE GENERAL HOSPITAL CORPORATIONInventor: Bakhos A. Tannous
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Patent number: 8383797Abstract: The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.Type: GrantFiled: November 13, 2006Date of Patent: February 26, 2013Assignees: Toyo Boseki Kabushiki Kaisha, National Institute of Advanced Industrial Science and TechnologyInventors: Yoshihiro Ohmiya, Yoshihiro Nakajima, Vadim Viviani, Shigeaki Nishii, Tomomi Asai
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Publication number: 20120122182Abstract: Described herein is a variant of wild type Gaussia luciferase that catalyzes glow-type emission kinetics suited for high-throughput functional screening applications. Polypeptides, functional fragments, variants, and nucleic acids that encode the enhanced luciferase are further described. One such polypeptide corresponds to wild type Gaussia luciferase with a substitution mutation of I for M at position 43 of the mature peptide. Methods of use, assay systems and kits that contain the polypeptides and/or nucleic acids are further described.Type: ApplicationFiled: June 30, 2010Publication date: May 17, 2012Applicant: THE GENERAL HOSPITAL CORPORATIONInventors: Bakhos A. Tannous, Casey Maguire
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Patent number: 6962986Abstract: Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.Type: GrantFiled: November 14, 2001Date of Patent: November 8, 2005Assignee: Toyo Boseki Kabushiki KaishaInventors: Vadim R. Viviani, Yoshihiro Ohmiya
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Patent number: 8642272Abstract: The present invention relates to an isolated nucleic acid and polypeptide sequence that encodes for a luciferase of Luciola italica, as well as mutants thereof. The luciferase proteins of the present invention have been found to have extended bioluminescence emission that is red- or blue-shifted, and are useful as a bioluminescent marker or as an additive to selected materials.Type: GrantFiled: October 1, 2010Date of Patent: February 4, 2014Assignee: Connecticut CollegeInventors: Bruce R. Branchini, Tara L. Southworth, Jennifer P. DeAngelis, Aldo Roda, Elisa Michelini
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Publication number: 20140273156Abstract: A modified beetle luciferase protein which is an environmentally sensitive reporter protein is provided.Type: ApplicationFiled: February 14, 2014Publication date: September 18, 2014Applicant: PROMEGA CORPORATIONInventors: Frank Fan, Martin Ken Lewis, John W. Shultz, Keith V. Wood, Braeden Butler
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Publication number: 20080299592Abstract: The compositions described herein shift the light output of luciferases to the near-IR by resonance energy transfer to a targetable near-IR fluorophore.Type: ApplicationFiled: February 29, 2008Publication date: December 4, 2008Inventor: Stephen C. Miller
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Publication number: 20140201855Abstract: Described are mutant luciferases, nucleic acids that encode them, cells and animals expressing them, methods of use thereof, and kits.Type: ApplicationFiled: February 9, 2012Publication date: July 17, 2014Applicant: UNIVERSITY OF MASSACHUSETTSInventors: Stephen C. Miller, David Mofford, Katryn Harwood
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Patent number: 8378086Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.Type: GrantFiled: July 31, 2012Date of Patent: February 19, 2013Inventors: Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
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Publication number: 20100105090Abstract: The invention relates to the nucleotide and amino acid sequences and to the activity and use of the secreted MLuc7 luciferase.Type: ApplicationFiled: January 26, 2008Publication date: April 29, 2010Applicant: BAYER HEALTHCARE AGInventors: Stefan Golz, Eugene Vysotski, Svetlana Markova, Anna Tumenceva
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Publication number: 20140223590Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.Type: ApplicationFiled: January 21, 2014Publication date: August 7, 2014Applicant: Promego CorporationInventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
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Patent number: 8669103Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.Type: GrantFiled: November 2, 2011Date of Patent: March 11, 2014Assignee: Promega CorporationInventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
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Patent number: 9840730Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.Type: GrantFiled: January 21, 2014Date of Patent: December 12, 2017Assignee: PROMEGA CORPORATIONInventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
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Patent number: 7939649Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.Type: GrantFiled: September 6, 2006Date of Patent: May 10, 2011Assignees: Stanford University, Regents of University of CaliforniaInventors: Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
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Publication number: 20110229955Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.Type: ApplicationFiled: March 24, 2011Publication date: September 22, 2011Applicants: The Board of Trustees of the Leland Stanford Junior University, The Regents of the University of CaliforniaInventors: Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
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Patent number: 8258277Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.Type: GrantFiled: March 22, 2012Date of Patent: September 4, 2012Assignees: The Board of Trustees of the Leland Stanford Junior University, The Regents of the University of CaliforniaInventors: Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
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Publication number: 20120295326Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.Type: ApplicationFiled: July 31, 2012Publication date: November 22, 2012Applicants: The Board of Trustees of the Leland Stanford Junior University, THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
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Publication number: 20120178141Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.Type: ApplicationFiled: March 22, 2012Publication date: July 12, 2012Applicants: The Regents of the University of California, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
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Publication number: 20120174242Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.Type: ApplicationFiled: November 2, 2011Publication date: July 5, 2012Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood