Search Patents
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Publication number: 20090233320Abstract: The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.Type: ApplicationFiled: December 9, 2004Publication date: September 17, 2009Applicant: NEC Soft, Ltd.Inventor: Hiromi Takenaka
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Patent number: 6132983Abstract: Proteins are provided having luciferase activity with greater heat stability than wildtype luciferases by replacing the glutamate equivalent to that at position 354 of Photinus pyralis luciferase or 356 of Luciola luciferases with an alternative amino acid, particularly lysine. DNA, vectors and cells that encode for and express the proteins are also provided as are test kits and reagents for carrying out luminescence assays using the proteins of the invention. Preferred proteins have a second replaced amino acid at a position equivalent to position 215 of Photinus pyralis luciferase or 217 of Luciola luciferases.Type: GrantFiled: November 20, 1996Date of Patent: October 17, 2000Assignee: The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern IrelandInventors: Christopher Robin Lowe, Peter John White, James Augusts Henry Murray, David James Squirrell
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Publication number: 20040142356Abstract: The luxA and luxB genes from P. luminescens which encode for the luciferase protein of the bacterial luciferase system were modified to generate codon-optimized versions that are optimized for expression in mammalian cells. The codon-optimized bacterial luciferase enzyme system genes of the invention can be used to develop a mammalian bioluminescence bioreporter useful in various medical research and diagnostics applications.Type: ApplicationFiled: October 30, 2003Publication date: July 22, 2004Inventors: Stacey Patterson, Rakesh Gupta, Gary Sayler, Hebe Dionisi
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Publication number: 20110039257Abstract: A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.Type: ApplicationFiled: April 2, 2007Publication date: February 17, 2011Applicant: Promega CorporationInventors: Brock Binkowski, Frank Fan, Susan Wigdal, Keith V. Wood, Monika G. Wood
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Patent number: 4833075Abstract: Luciferase is immobilized on a membrane prepared from an absorbent albuminoid such as procine skin gelatin. The membrane may be formed by spreading a solution of porcine skin gelatin on a support and after incubation and drying removing the resultant membrane from the support. Preferably, the membrane is crosslinked with a solution of glutaraldehyde, and luciferase is immobilized by saturating the membrane with luciferase in the presence of dithiothreitol. The immobilized luciferase is particularly suitable for the quantitative determination of adenosine triphosphoric acid (ATP).Type: GrantFiled: April 1, 1988Date of Patent: May 23, 1989Assignee: Centre National de la Recherche ScientifiqueInventors: Mookambeswaran Vijayalakshmi, Rajgopal Sunanda
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Publication number: 20040063165Abstract: There is provided a process of inducing luminescent emission from a luciferase bioluminescent reaction particularly useful in binding assays. A luciferase bioluminescent combination, together with an inactive, caged trigger compound such as a cofactor, is subjected to photonic radiation, so as to release the trigger compound in active form, and thereby cause substantially instantaneous reaction of the active trigger compound so released with the luciferase combination, to induce photonic emission which can be detected and measured.Type: ApplicationFiled: June 12, 2003Publication date: April 1, 2004Inventor: Yahia A. Gawad
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Publication number: 20020081644Abstract: A polynucleotide encoding a secreted form of wild type Renilla luciferase. Also provided is a polynucleotide encoding a secreted modified form of wild type Renilla luciferase. Additionally, the polypeptides encoded by the polynucleotides of the present invention and uses of the polynucleotides and polypeptides of the present invention in biological assays. Also, a stable mammalian packaging cell line which produces retroviruses carrying a polynucleotide encoding a secreted Renilla luciferase.Type: ApplicationFiled: March 14, 2001Publication date: June 27, 2002Inventors: Alan P. Escher, Jingxue Liu
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Publication number: 20100068739Abstract: Use of a luciferase that has a mutation of at least one amino acid selected from the group consisting of positions 14, 35, 182, 232 and 465, where the numbering is according to the sequence of the luciferase from P. pyralis (SEQ ID NO:1) in a method that is performed at a pH below the optimal pH for the wild-type luciferase during at least part of the time period over which bioluminescence measurements are taken, wherein the specific activity of the mutant luciferase is higher than the specific activity of wild-type at the pH at which the method is carried out.Type: ApplicationFiled: August 9, 2006Publication date: March 18, 2010Inventors: Laurence Tisi, Gim Hoong Erica Law, Olga Gandelman, James Augustus Henry Murray
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Publication number: 20040219622Abstract: A composition for the chemiluminescent assay of the activity of a luciferase comprising (i) a substrate for the luciferase, which, upon enzymatic reaction with the luciferase in the presence of any required cosubstrate and/or cofactor, yields a detectable chemiluminescent singal, (ii) any cosubstrate and/or cofactor required or beneficial for enzymatic activity of the luciferase, and, as an improvement of the composition, (iii) a water-soluble, organic phosphine-containing compound, which enables the light output from the enzymatic reaction to be modulated. The composition can be used in applications designed to quantitate the presence of the enzyme(s) itself, either in single or dual format, or in applications designed to quantitate the presence of a required cosubstrate, such as ATP.Type: ApplicationFiled: June 23, 2004Publication date: November 4, 2004Inventor: M. Dean Savage
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Publication number: 20020102687Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector.Type: ApplicationFiled: April 26, 2001Publication date: August 1, 2002Applicant: CHISSO CORPORATIONInventor: Satoshi Inouye
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Patent number: 7906298Abstract: A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis.Type: GrantFiled: October 26, 1999Date of Patent: March 15, 2011Assignee: Promega CorporationInventors: David J Squirrell, Melenie J Murphy, Rachel L Price, Christopher R Lowe, Peter J White, Laurence C Tisi, James A H Murray
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Patent number: 7871803Abstract: The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.Type: GrantFiled: December 9, 2004Date of Patent: January 18, 2011Assignee: NEC Soft, Ltd.Inventor: Hiromi Takenaka
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Publication number: 20040235077Abstract: The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.Type: ApplicationFiled: April 22, 2004Publication date: November 25, 2004Applicant: Kikkoman CorporationInventors: Noriaki Hattori, Seiji Murakami
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Publication number: 20110015095Abstract: Nucleotide and amino acid sequences of luciferase peptides that are encoded by genes within the genome of Arachnocampa (Diptera) are disclosed. Specifically provided are functional ATP-dependent luciferases that catalyze luminescence reactions with emission spectra within the blue portion of the spectrum. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and active subsequences of the enzyme peptides, and methods of identifying modulators and substrates of the luciferase peptides. Methods of assays, including multiple reporter assays utilizing at least two ATP-dependent luciferases are provided.Type: ApplicationFiled: August 18, 2006Publication date: January 20, 2011Inventors: Virginia Leitch, Mira Maria Dumancic, Stephen Charles Trowell, Helen Dacres
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Patent number: 7915021Abstract: This invention relates to: the development of a mutant firefly luciferase in order to use dATP as a DNA polymerase substrate upon pyrosequencing, such luciferase being subjected to substrate specificity modification in a manner such that the dATP-induced activity alone is decreased while the ATP-induced activity is maintained; and a mutant firefly luciferase for which the proportion of activity induced by dATP to activity induced by ATP (dATP/ATP) is lower than that for the wild-type firefly luciferase, in which an amino acid identified based on homology analysis as corresponding with the 421st amino acid (glycine) of the amino acid sequence of the wild-type North American firefly (Photinus pyralis) luciferase has been substituted with a polar amino acid.Type: GrantFiled: August 20, 2008Date of Patent: March 29, 2011Assignee: Hitachi, Ltd.Inventors: Hiroyuki Tsunoda, Hideki Kambara
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Publication number: 20040002127Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector.Type: ApplicationFiled: March 4, 2003Publication date: January 1, 2004Applicant: CHISSO CORPORATIONInventor: Satoshi Inouye
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Publication number: 20110081670Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: ApplicationFiled: June 21, 2010Publication date: April 7, 2011Applicant: PROMEGA CORPORATIONInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
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Patent number: 7560245Abstract: The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.Type: GrantFiled: April 22, 2004Date of Patent: July 14, 2009Assignee: Kikkoman CorporationInventors: Noriaki Hattori, Seiji Murakami
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Publication number: 20020119542Abstract: Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.Type: ApplicationFiled: November 14, 2001Publication date: August 29, 2002Inventors: Vadim R. Viviani, Yoshihiro Ohmiya
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Patent number: 8361739Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.Type: GrantFiled: June 21, 2010Date of Patent: January 29, 2013Assignee: Promega CorporationInventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood