Search Patents
  • Patent number: 11306295
    Abstract: A new thermostable luciferase of the following mutant luciferase (a) or (b): (a) a mutant of a wild-type luciferase comprising the amino acid sequence of SEQ ID NO: 1, wherein phenylalanine at position 292 and/or phenylalanine at position 294 in the amino acid sequence of SEQ ID NO: 1 is substituted with another amino acid; or (b) a mutant of a luciferase having 93% or more homology with the amino acid sequence of SEQ ID NO: 1, wherein in the amino acid sequence of the mutant, the amino acid at a site corresponding to position 292 and/or position 294 in the amino acid sequence of SEQ ID NO: 1 is substituted with another amino acid.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: April 19, 2022
    Assignees: TOYO B-NET CO., LTD., TOYO INK SC HOLDINGS CO., LTD.
    Inventors: Ayumi Hori, Yutaka Yamagishi
  • Publication number: 20090305353
    Abstract: The present invention provides a mutant firefly luciferase consisting of a mutant amino acid sequence derived from the amino acid sequence of a wild-type firefly luciferase by at least substitution (a), (b), or (c) below and having luminescence intensity higher than that of the wild-type firefly luciferase.
    Type: Application
    Filed: September 6, 2006
    Publication date: December 10, 2009
    Inventors: Hiroya Fujii, Kenichi Noda
  • Patent number: 6451549
    Abstract: A polynucleotide encoding a secreted form of wild type Renilla luciferase. Also provided is a polynucleotide encoding a secreted modified form of wild type Renilla luciferase. Additionally, the polypeptides encoded by the polynucleotides of the present invention and uses of the polynucleotides and polypeptides of the present invention in biological assays. Also, a stable mammalian packaging cell line which produces retroviruses carrying a polynucleotide encoding a secreted Renilla luciferase.
    Type: Grant
    Filed: March 14, 2001
    Date of Patent: September 17, 2002
    Assignee: Loma Linda University
    Inventors: Alan P. Escher, Jingxue Liu
  • Patent number: 6544754
    Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector. Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein. These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.
    Type: Grant
    Filed: April 26, 2001
    Date of Patent: April 8, 2003
    Assignee: Chisso Corporation
    Inventor: Satoshi Inouye
  • Patent number: 6495355
    Abstract: Nucleic acid compositions and polypeptides encoding a red-shifted form of firefly luciferase are provided. These red-shifted luciferases are characterized by spectrum of light emission having detectable emissions at 610 nm (luc610), preferably a primary peak at 610 nm. The nucleic acid compositions find use in various systems as a reporter gene, and are of particular interest for use as a reporter with in vivo systems, because of the efficient transfer of red light through tissues. The red-shifted luciferase may be combined in such assays with luciferases emitting at other spectra, in order to monitor multiple processes simultaneously.
    Type: Grant
    Filed: June 21, 2000
    Date of Patent: December 17, 2002
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Christopher Contag, Brian Eames
  • Patent number: 8980603
    Abstract: Described herein is a variant of wild type Gaussia luciferase that catalyzes glow-type emission kinetics suited for high-throughput functional screening applications. Polypeptides, functional fragments, variants, and nucleic acids that encode the enhanced luciferase are further described. One such polypeptide corresponds to wild type Gaussia luciferase with a substitution mutation of I for M at position 43 of the mature peptide. Methods of use, assay systems and kits that contain the polypeptides and/or nucleic acids are further described.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: March 17, 2015
    Assignee: The General Hospital Corporation
    Inventors: Bakhos A. Tannous, Casey Maguire
  • Publication number: 20140273156
    Abstract: A modified beetle luciferase protein which is an environmentally sensitive reporter protein is provided.
    Type: Application
    Filed: February 14, 2014
    Publication date: September 18, 2014
    Applicant: PROMEGA CORPORATION
    Inventors: Frank Fan, Martin Ken Lewis, John W. Shultz, Keith V. Wood, Braeden Butler
  • Patent number: 7125697
    Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector. Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein. These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.
    Type: Grant
    Filed: March 4, 2003
    Date of Patent: October 24, 2006
    Assignee: Chisso Corporation
    Inventor: Satoshi Inouye
  • Patent number: 6812012
    Abstract: The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.
    Type: Grant
    Filed: June 26, 2000
    Date of Patent: November 2, 2004
    Assignee: Kikkoman Corporation
    Inventors: Noriaki Hattori, Seiji Murakami
  • Patent number: 10851141
    Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.
    Type: Grant
    Filed: June 19, 2017
    Date of Patent: December 1, 2020
    Assignee: DKK-TOA Corporation
    Inventors: Kenichi Noda, Satoshi Yawata, Ai Shimomura
  • Publication number: 20120122182
    Abstract: Described herein is a variant of wild type Gaussia luciferase that catalyzes glow-type emission kinetics suited for high-throughput functional screening applications. Polypeptides, functional fragments, variants, and nucleic acids that encode the enhanced luciferase are further described. One such polypeptide corresponds to wild type Gaussia luciferase with a substitution mutation of I for M at position 43 of the mature peptide. Methods of use, assay systems and kits that contain the polypeptides and/or nucleic acids are further described.
    Type: Application
    Filed: June 30, 2010
    Publication date: May 17, 2012
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventors: Bakhos A. Tannous, Casey Maguire
  • Patent number: 7276363
    Abstract: Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.
    Type: Grant
    Filed: September 14, 2004
    Date of Patent: October 2, 2007
    Assignee: Toyo Boseki Kabushiki Kaisha
    Inventors: Vadim R. Viviani, Yoshihiro Ohmiya
  • Patent number: 6962986
    Abstract: Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.
    Type: Grant
    Filed: November 14, 2001
    Date of Patent: November 8, 2005
    Assignee: Toyo Boseki Kabushiki Kaisha
    Inventors: Vadim R. Viviani, Yoshihiro Ohmiya
  • Publication number: 20090047219
    Abstract: An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.
    Type: Application
    Filed: August 7, 2008
    Publication date: February 19, 2009
    Inventors: Yoshihiro Ohmiya, Chun Wu
  • Patent number: 6171808
    Abstract: Proteins are provided having luciferase activity with lower Km than wild-type luciferases by altering the amino acid residue at position 270 of the wild-type to an amino acid other than glutamate. Greater heat stability than wild-type luciferases while retaining the lower Km is provided by also replacing the glutamate equivalent to that at position 354 of Photinus pyralis luciferase or 356 of Luciola luciferases with an alternative amino acid, particularly lysine and/or the amino acid residue at 215 of Photinus pyralis and 217 of the Luciola species with a hydrophobic amino acid. DNA, vectors and cells that encode for and express the proteins are also provided as are test kits and reagents for carrying out luminescence assays using the proteins of the invention.
    Type: Grant
    Filed: October 1, 1997
    Date of Patent: January 9, 2001
    Assignee: The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland
    Inventors: David J Squirrell, Christopher R Lowe, Peter J White, James A H Murray
  • Patent number: 6232107
    Abstract: Isolated and purified nucleic acid molecules that encode a luciferase from Renilla mulleri, Gaussia and Pleuromamma, and the proteins encoded thereby are provided. Isolated and purified nucleic acids encoding green fluorescent proteins from the genus Renilla and Ptilosarcus, and the green fluorescent proteins encoded thereby are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.
    Type: Grant
    Filed: March 26, 1999
    Date of Patent: May 15, 2001
    Assignees: Prolume, LTD
    Inventors: Bruce J. Bryan, Christopher Szent-Gyorgyi
  • Publication number: 20110171669
    Abstract: The present invention relates to an isolated nucleic acid and polypeptide sequence that encodes for a luciferase of Luciola italica, as well as mutants thereof. The luciferase proteins of the present invention have been found to have extended bioluminescence emission that is red- or blue-shifted, and are useful as a bioluminescent marker or as an additive to selected materials.
    Type: Application
    Filed: October 1, 2010
    Publication date: July 14, 2011
    Inventors: Bruce R. Branchini, Tara L. Southworth, Jennifer P. DeAngelis, Aldo Roda, Elisa Michelini
  • Patent number: 8642272
    Abstract: The present invention relates to an isolated nucleic acid and polypeptide sequence that encodes for a luciferase of Luciola italica, as well as mutants thereof. The luciferase proteins of the present invention have been found to have extended bioluminescence emission that is red- or blue-shifted, and are useful as a bioluminescent marker or as an additive to selected materials.
    Type: Grant
    Filed: October 1, 2010
    Date of Patent: February 4, 2014
    Assignee: Connecticut College
    Inventors: Bruce R. Branchini, Tara L. Southworth, Jennifer P. DeAngelis, Aldo Roda, Elisa Michelini
  • Patent number: 8673558
    Abstract: A modified beetle luciferase protein which is an environmentally sensitive reporter protein is provided.
    Type: Grant
    Filed: April 24, 2012
    Date of Patent: March 18, 2014
    Assignee: Promega Corporation
    Inventors: Frank Fan, Martin Ken Lewis, John W. Shultz, Keith V. Wood, Braeden Butler
  • Patent number: 7807429
    Abstract: The present invention relates to an isolated nucleic acid and polypeptide sequence that encodes for a luciferase of Luciola italica, as well as mutants thereof. The luciferase proteins of the present invention have been found to have extended bioluminescence emission that is red- or blue-shifted, and are useful as a bioluminescent marker or as an additive to selected materials.
    Type: Grant
    Filed: February 6, 2007
    Date of Patent: October 5, 2010
    Assignee: Connecticut College
    Inventors: Bruce R. Branchini, Tara L. Southworth, Jennifer P. DeAngelis, Aldo Roda, Elisa Michelini