Abstract: Genetic material encoding luciferase from the marine coelenterate Renilla has been isolated and characterized. This genetic material allows the production of peptides for use as labels in bioluminescence assays or can itself be directly used to identify luciferase genes from related organisms.
Type:
Grant
Filed:
December 14, 1993
Date of Patent:
May 23, 1995
Assignee:
University of Georgia Research Foundation, Inc.
Abstract: Genetic material encoding luciferase from the marine coelenterate Renilla has been isolated and characterized. This genetic material allows the production of peptides for use as labels in bioluminescence assays or can itself be directly used to identify luciferase genes from related organisms.
Type:
Grant
Filed:
June 17, 1993
Date of Patent:
March 8, 1994
Assignee:
University of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center
Abstract: Isolated and purified nucleic acid molecules that encode a luciferase from Renilla mulleri, Gaussia and Pleuromamma, and the proteins encoded thereby are provided. Isolated and purified nucleic acids encoding green fluorescent proteins from the genus Renilla and Ptilosarcus, and the green fluorescent proteins encoded thereby are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.
Type:
Grant
Filed:
June 30, 2000
Date of Patent:
August 20, 2002
Assignee:
Prolume, Ltd.
Inventors:
Bruce J. Bryan, Christopher Szent-Gyorgyi
Abstract: The compositions described herein shift the light output of luciferases to the near-IR by resonance energy transfer to a targetable near-IR fluorophore.
Abstract: DNA compositions and methods of constructing and using the same consisting of DNA sequences encoding luciferase activity, or DNA sequences encoding hybrid molecules exhibiting luciferase activity and a second biological activity that are useful in performing biological assays.
Type:
Grant
Filed:
June 2, 1995
Date of Patent:
October 7, 1997
Assignee:
The Regents of the University of California
Inventors:
Marlene DeLuca McElroy, deceased, Donald Raymond Helinski, Keith Vernon Wood, Jeffrey Roux De Wet, David Wing Ow, Stephen Herbert Howell
Abstract: The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22.
Abstract: The present invention provides luciferase with an amino acid sequence shown in FIG. 1, a gene encoding the the same, a recombinant vector DNA comprising the gene ligated at a site downstream of a promoter which can be expressed in a host cell, a transformant prepared by transforming the host cell with the recombinant vector DNA and a process of producing luciferase using the transformant.
Type:
Grant
Filed:
June 15, 1994
Date of Patent:
February 18, 1997
Assignee:
Toray Industries, Inc.
Inventors:
Jun Kazami, Haruji Nakamura, Toshio Goto, deceased
Abstract: Luciferases which are different from those known heretofore have been desired. A luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from the group consisting of valine at the position of 44, alanine at the position of 54 and tyrosine at the position of 138 is substituted with other amino acid(s) in the amino acid sequence of SEQ ID NO: 2.
Abstract: The present invention provides a detection method and a method of manufacturing a detection kit, both characterized by use of an organic sulfur reagent, and which are effective at low concentration of the reagent, are inexpensive, and have reduced unpleasent odor. Provided is a reagent kit for detecting a Coleoptera luciferase, comprising an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion. Also provide is a method for detecting a Coleoptera luciferase, comprising step 1 of mixing an aqueous solution, containing an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion, with a sample containing a Coleoptera luciferase, to give a mixed solution; and step 2 of measuring the light emitted in the mixed solution.
Abstract: The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22.
Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.
Type:
Application
Filed:
September 6, 2006
Publication date:
May 28, 2009
Inventors:
Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu
Abstract: A process for producing a cloned luciferase-synthesizing microorganism, capable of the expression of bioluminescence, is disclosed. The process involves isolation and digestion of the genomic DNA of an appropriate bioluminescent microorganism, such as Vibrio harveyi, to obtain a DNA fragment which codes for luciferase. The DNA fragment is inserted into a pre-existing cloning vehicle under the control of an expression promoter, thereby producing a recombinant or derivatized cloning vehicle. A host microorganism is transformed with the derivatized cloning vehicle, cultured and purified for the expression of bioluminescence.
Abstract: The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22.
Abstract: An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.
Type:
Grant
Filed:
August 7, 2008
Date of Patent:
March 27, 2012
Assignee:
National Institute of Advanced Industrial Science and Technology
Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
Type:
Application
Filed:
January 21, 2014
Publication date:
August 14, 2014
Applicant:
PROMEGA CORPORATION
Inventors:
Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
Abstract: A modified form of beetle luciferase, which has been engineered for improved genetic reporting, is disclosed. The modified form contains one or more new features. Chief among these is removal of the peroxisomal translocation sequence to yield a cytoplasmic form of the enzyme. Other changes include removal of potentially interfering restriction sites and genetic regulatory sites from the gene, improvement of the codon usage for mammalian cells. The modified luciferase reporter enzyme is also devoid of potential N-glycosylation targets to minimize post-translational modification and remains in the cytoplasm of host cells to optimize substrate availability.
Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.
Type:
Application
Filed:
March 24, 2011
Publication date:
September 22, 2011
Applicants:
The Board of Trustees of the Leland Stanford Junior University, The Regents of the University of California
Inventors:
Sanjiv S. Gambhir, Andreas M. Loening, Anna M. Wu