Search Patents
  • Thermal stable luciferase with improved resistance to inhibition by luciferin break-down products
    Patent number: 11261478
    Abstract: Provided herein are inhibitor-resistant luciferase mutants, and methods of use thereof. In particular, luciferase mutants are provided that are thermal stable and exhibit improved stability in the presence of luciferin break-down products, such as dehydroluciferin. Further provided are assay systems comprising inhibitor-resistant luciferase mutants and amino acid sequences of the inhibitor-resistant luciferase mutants.
    Type: Grant
    Filed: October 13, 2017
    Date of Patent: March 1, 2022
    Assignee: Promega Corporation
    Inventors: Ce Shi, Thomas Kirkland, Poncho Meisenheimer, Lance P. Encell, Mary Hall
  • Mutant luciferases
    Patent number: 6387675
    Abstract: The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: May 14, 2002
    Assignee: Promega Corporation
    Inventors: Keith V. Wood, Monika G. Gruber
  • Mutant firefly luciferase
    Patent number: 7915021
    Abstract: This invention relates to: the development of a mutant firefly luciferase in order to use dATP as a DNA polymerase substrate upon pyrosequencing, such luciferase being subjected to substrate specificity modification in a manner such that the dATP-induced activity alone is decreased while the ATP-induced activity is maintained; and a mutant firefly luciferase for which the proportion of activity induced by dATP to activity induced by ATP (dATP/ATP) is lower than that for the wild-type firefly luciferase, in which an amino acid identified based on homology analysis as corresponding with the 421st amino acid (glycine) of the amino acid sequence of the wild-type North American firefly (Photinus pyralis) luciferase has been substituted with a polar amino acid.
    Type: Grant
    Filed: August 20, 2008
    Date of Patent: March 29, 2011
    Assignee: Hitachi, Ltd.
    Inventors: Hiroyuki Tsunoda, Hideki Kambara
  • Luciferase assay method
    Patent number: 5283179
    Abstract: The invention provides improved methods for assaying samples for the presence of a beetle luciferase. The methods of the invention entail improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions.
    Type: Grant
    Filed: September 10, 1990
    Date of Patent: February 1, 1994
    Assignee: Promega Corporation
    Inventor: Keith V. Wood
  • Star-worm luciferase
    Patent number: 9234186
    Abstract: The present invention relates to a luciferase derived from a star-worm belonging to genus Diplocladon, the luciferase inducing luminescence such that a maximum luminous wavelength falls within a range of 557 to 562 nm over the entire pH range of 5.5 to 8.0, or the luciferase inducing luminescence having 1.5 times the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: January 12, 2016
    Assignees: OLYMPUS CORPORATION, NIMURA GENETIC SOLUTIONS CO., LTD., PERAK STATE DEVELOPMENT CORPORATION
    Inventors: Ryutaro Akiyoshi, Katsunori Ogo, Hirobumi Suzuki
  • Mutant firefly luciferase, its gene and method for producing mutant firefly luciferase
    Patent number: 8748144
    Abstract: A Heike firefly luciferase having excellent thermostability and storage stability and a process for its production, wherein the amino acid corresponding to position 287 of Heike firefly luciferase is alanine.
    Type: Grant
    Filed: February 16, 2010
    Date of Patent: June 10, 2014
    Assignee: Kikkoman Corporation
    Inventors: Yukako Kodama, Eriko Yoshihara
  • Luminescence method for luciferin/luciferase system and luminescent reagent
    Patent number: 6060261
    Abstract: A luminescence method for a luciferin/luciferase system which comprises reacting a luciferin solution containing an increased amount of dissolved carbon dioxide with luciferase; and a luciferase assay reagent comprising a luciferin solution containing an increased amount of dissolved carbon dioxide. Thus, a method of luminescent assay excellent in continuity and stability is provided as a substitute for the conventional luminescent luciferase assay featured by rapidity and high sensitivity.
    Type: Grant
    Filed: April 30, 1999
    Date of Patent: May 9, 2000
    Assignee: Toyo Ink Mfg. Co., Ltd.
    Inventors: Masayuki Ryufuku, Hozumi Tanaka, Hideyuki Takeuchi
  • ENGINEERED LUCIFERASES
    Publication number: 20090286299
    Abstract: DNA sequencing techniques are important for a variety of research and diagnostic applications. Pyrosequencing is a “sequencing by synthesis” technique that makes use of luciferase. Modified luciferase enzymes and methods of DNA pyrosequencing are provided. Means of preparing and producing mutant luciferases that have enhanced selectivity for ATP or dATP are described.
    Type: Application
    Filed: May 15, 2009
    Publication date: November 19, 2009
    Inventors: Mostafa Ronaghi, Helmy A. Eltoukhy, Leila Bazargan
  • Synthetic luciferase gene and protein
    Publication number: 20140087402
    Abstract: A codon optimized and stabilized luciferase gene and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.
    Type: Application
    Filed: December 4, 2012
    Publication date: March 27, 2014
    Applicant: Marker Gene Technologies, Inc.
    Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook, Ying Jiang
  • LUCIFERASE SIGNAL ENHANCING COMPOSITIONS
    Publication number: 20100055693
    Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.
    Type: Application
    Filed: October 24, 2007
    Publication date: March 4, 2010
    Applicant: GENE STREAM PTY LTD.
    Inventors: Marco Peter Leu, John Michael Daly
  • Immobilized luciferase for the quantitative determination of ATP
    Patent number: 4833075
    Abstract: Luciferase is immobilized on a membrane prepared from an absorbent albuminoid such as procine skin gelatin. The membrane may be formed by spreading a solution of porcine skin gelatin on a support and after incubation and drying removing the resultant membrane from the support. Preferably, the membrane is crosslinked with a solution of glutaraldehyde, and luciferase is immobilized by saturating the membrane with luciferase in the presence of dithiothreitol. The immobilized luciferase is particularly suitable for the quantitative determination of adenosine triphosphoric acid (ATP).
    Type: Grant
    Filed: April 1, 1988
    Date of Patent: May 23, 1989
    Assignee: Centre National de la Recherche Scientifique
    Inventors: Mookambeswaran Vijayalakshmi, Rajgopal Sunanda
  • Phosphine-containing formulations for chemiluminescent luciferase assays
    Publication number: 20040219622
    Abstract: A composition for the chemiluminescent assay of the activity of a luciferase comprising (i) a substrate for the luciferase, which, upon enzymatic reaction with the luciferase in the presence of any required cosubstrate and/or cofactor, yields a detectable chemiluminescent singal, (ii) any cosubstrate and/or cofactor required or beneficial for enzymatic activity of the luciferase, and, as an improvement of the composition, (iii) a water-soluble, organic phosphine-containing compound, which enables the light output from the enzymatic reaction to be modulated. The composition can be used in applications designed to quantitate the presence of the enzyme(s) itself, either in single or dual format, or in applications designed to quantitate the presence of a required cosubstrate, such as ATP.
    Type: Application
    Filed: June 23, 2004
    Publication date: November 4, 2004
    Inventor: M. Dean Savage
  • Luciferase labelling method
    Patent number: 5837465
    Abstract: Luciferase is conjugated to a chemical entity, particularly to a specific binding agent such as an antibody, antigen or a nucleic acid, and more particularly an antibody, by (a) mixing the luciferase with one or more of D-luciferin, magnesium ions and adenosine triphosphate and (b) performing a covalent coupling reaction between the luciferase and the binding reagent using a covalent coupling reagent where the amount of D-luciferin, magnesium ions and/or adenosine triphosphate is sufficient to protect the luciferase activity against inhibition by the covalent coupling reagent. Preferably, step (a) is carried out by mixing the luciferase with its substrates in solution and preferably both magnesium and adenosine triphosphate are present as magnesium adenosine triphosphate (Mg.sup.2+ ATP), optionally together with D-luciferin. Also disclosed is a labeled chemical entity comprising a chemical entity conjugated to active luciferase as formed by the method.
    Type: Grant
    Filed: February 28, 1997
    Date of Patent: November 17, 1998
    Assignee: The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland of Defence Evaluation & Research Agency
    Inventors: David James Squirrell, Melanie Jane Murphy
  • FUSION PROTEINS OF BACTERIAL LUCIFERASE AS MULTICOLOR LUMINESCENT SENSORS
    Publication number: 20130023027
    Abstract: The present invention discloses systems and methods for altering the color of bacterial bioluminescence via a fusion protein complex by fusing Discosoma sp. fluorescent protein mOrange (mOrange) with Vibrio harveyi luciferase. The fusion of mOrange to the N- or C-terminus of either ? or ? subunit of luciferase, via a short peptide linker produces fully active fusion enzymes. The fusion of mOrange to the N-terminus of luciferase ? gives rise to a new 560-nm emission component. The same methodology may be used to alter bacterial bioluminescence color by covalent attachment of other suitable fluorescent proteins or chromophores to luciferase for generating multi-color sensors.
    Type: Application
    Filed: June 24, 2012
    Publication date: January 24, 2013
    Inventor: Shiao-Chun Tu
  • PH Tolerant Luciferase
    Publication number: 20100068739
    Abstract: Use of a luciferase that has a mutation of at least one amino acid selected from the group consisting of positions 14, 35, 182, 232 and 465, where the numbering is according to the sequence of the luciferase from P. pyralis (SEQ ID NO:1) in a method that is performed at a pH below the optimal pH for the wild-type luciferase during at least part of the time period over which bioluminescence measurements are taken, wherein the specific activity of the mutant luciferase is higher than the specific activity of wild-type at the pH at which the method is carried out.
    Type: Application
    Filed: August 9, 2006
    Publication date: March 18, 2010
    Inventors: Laurence Tisi, Gim Hoong Erica Law, Olga Gandelman, James Augustus Henry Murray
  • MUTANT BEETLE LUCIFERASE, GENE, RECOMBINANT VECTOR, TRANSFORMANT, AND METHOD FOR PREPARING MUTANT BEETLE LUCIFERASE
    Publication number: 20190352350
    Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.
    Type: Application
    Filed: June 19, 2017
    Publication date: November 21, 2019
    Applicant: DKK-TOA Corporation
    Inventors: Kenichi Noda, Satoshi Yawata, Ai Shimomura
  • LUCIFERASE-BASED ASSAYS
    Publication number: 20130217051
    Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Application
    Filed: January 25, 2013
    Publication date: August 22, 2013
    Applicant: PROMEGA CORPORATION
    Inventor: Promega Corporation
  • ARACHNOCAMPA LUCIFERASES
    Publication number: 20110015095
    Abstract: Nucleotide and amino acid sequences of luciferase peptides that are encoded by genes within the genome of Arachnocampa (Diptera) are disclosed. Specifically provided are functional ATP-dependent luciferases that catalyze luminescence reactions with emission spectra within the blue portion of the spectrum. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and active subsequences of the enzyme peptides, and methods of identifying modulators and substrates of the luciferase peptides. Methods of assays, including multiple reporter assays utilizing at least two ATP-dependent luciferases are provided.
    Type: Application
    Filed: August 18, 2006
    Publication date: January 20, 2011
    Inventors: Virginia Leitch, Mira Maria Dumancic, Stephen Charles Trowell, Helen Dacres
  • Luciferase signal enhancing compositions
    Patent number: RE46199
    Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.
    Type: Grant
    Filed: July 31, 2014
    Date of Patent: November 8, 2016
    Assignee: GENE STREAM PTY LTD.
    Inventors: Marco Peter Leu, John Michael Daly
  • Luciferase signal enhancing compositions
    Patent number: RE47607
    Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.
    Type: Grant
    Filed: October 13, 2016
    Date of Patent: September 17, 2019
    Assignee: GENE STREAM PTY LTD.
    Inventors: Marco Peter Leu, John Michael Daly
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