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  • METHODS, REAGENTS AND KITS FOR LUCIFERASE ASSAY
    Publication number: 20110256564
    Abstract: The invention relates to methods, reagents and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel luciferase assay system with reduced background luminescence to allow for increased detection sensitivity. Provided is a method of detecting luciferase activity in a sample using coelenterazine or an analog thereof as a substrate, comprising: (a) initiating luciferase-catalyzed luminescence production by contacting said sample with a luciferase detection reagent to yield a reaction mixture, said reagent comprising coelenterazine and at least one iodide source in an amount sufficient to reduce the autoluminescence of said coelenterazine, (b) incubating said reagent mixture under conditions suitable to produce luminescence, and (c) measuring the luminescence produced. Also provided are detections reagents and kits for use in such a method.
    Type: Application
    Filed: October 29, 2008
    Publication date: October 20, 2011
    Applicant: Perkinelmer Health Science B.V.
    Inventor: Harry Van Lune
  • Methods, reagents and kits for luciferase assay
    Patent number: 8512968
    Abstract: The invention relates to methods, reagents and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel luciferase assay system with reduced background luminescence to allow for increased detection sensitivity. Provided is a method of detecting luciferase activity in a sample using coelenterazine or an analog thereof as a substrate, comprising: (a) initiating luciferase-catalyzed luminescence production by contacting said sample with a luciferase detection reagent to yield a reaction mixture, said reagent comprising coelenterazine and at least one iodide source in an amount sufficient to reduce the autoluminescence of said coelenterazine, (b) incubating said reagent mixture under conditions suitable to produce luminescence, and (c) measuring the luminescence produced. Also provided are detections reagents and kits for use in such a method.
    Type: Grant
    Filed: October 29, 2008
    Date of Patent: August 20, 2013
    Assignee: Perkinelmer Health Sciences B.V.
    Inventor: Harry Van Lune
  • Red-shifted luciferase
    Patent number: 6495355
    Abstract: Nucleic acid compositions and polypeptides encoding a red-shifted form of firefly luciferase are provided. These red-shifted luciferases are characterized by spectrum of light emission having detectable emissions at 610 nm (luc610), preferably a primary peak at 610 nm. The nucleic acid compositions find use in various systems as a reporter gene, and are of particular interest for use as a reporter with in vivo systems, because of the efficient transfer of red light through tissues. The red-shifted luciferase may be combined in such assays with luciferases emitting at other spectra, in order to monitor multiple processes simultaneously.
    Type: Grant
    Filed: June 21, 2000
    Date of Patent: December 17, 2002
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Christopher Contag, Brian Eames
  • Modified luciferase nucleic acids and methods of use
    Publication number: 20040142356
    Abstract: The luxA and luxB genes from P. luminescens which encode for the luciferase protein of the bacterial luciferase system were modified to generate codon-optimized versions that are optimized for expression in mammalian cells. The codon-optimized bacterial luciferase enzyme system genes of the invention can be used to develop a mammalian bioluminescence bioreporter useful in various medical research and diagnostics applications.
    Type: Application
    Filed: October 30, 2003
    Publication date: July 22, 2004
    Inventors: Stacey Patterson, Rakesh Gupta, Gary Sayler, Hebe Dionisi
  • SCREENING DEVICE CONTAINING NANOG PROMOTER, SOX2 PROMOTER, LIN 28 PROMOTER, OCT4 PROMOTER AND LUCIFERASE GENE AND SCREENING METHOD THEREOF
    Publication number: 20130004959
    Abstract: A screening device that contains Nanog promoter, Sox2 promoter, Lin28 promoter, Oct4 promoter and luciferase gene and a method thereof is revealed. The screening device is used to find out small molecules that improve activity of the promoters mentioned-above. A reporter plasmid formed by a pStable vector is introduced into stable somatic cells or stem cells with reporter plasmids such as a C2C12 mouse myoblast cell line and a P19 embryonal carcinoma cell line. By the luciferase gene contained in the pStable vector and pluripotent gene promoters placed upstream of the luciferase gene, luciferase expression is assayed by luminescence intensity measured. Thus response and activity of the promoters placed upstream of the luciferase gene is measured and small molecules inducing pluripotency are found out. If a drug can activate all promoters or respective promoter, it can be used as a drug that induces cell pluropatency.
    Type: Application
    Filed: January 3, 2012
    Publication date: January 3, 2013
    Applicant: NATIONAL CENTRAL UNIVERSITY
    Inventors: Shen Laing CHEN, Wei Jhen HUANG, Moo Rung LOO
  • Mutated genes for the catalytic protein of luciferase and use thereof
    Patent number: 10377995
    Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.
    Type: Grant
    Filed: December 7, 2017
    Date of Patent: August 13, 2019
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Yuiko Miura, Junichi Sato
  • Mutated genes for the catalytic protein of luciferase and use thereof
    Patent number: 10988741
    Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.
    Type: Grant
    Filed: May 6, 2019
    Date of Patent: April 27, 2021
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Yuiko Miura, Junichi Sato
  • LUCIFERASE SHOWING ORANGE LUMINESCENCE
    Publication number: 20170204379
    Abstract: A luciferase according to an embodiment comprises an amino acid sequence in which a mutation is introduced into an amino acid sequence of SEQ ID NO: 38. The luciferase catalyzes a luminescence reaction that generates luminescence having the maximum luminescent wavelength of 570 nm to 610 nm. The luminescence has an intensity at least 10 times higher than that of luminescence generated in a luminescence reaction catalyzed by a luciferase having an amino acid sequence of SEQ ID NO: 48.
    Type: Application
    Filed: March 29, 2017
    Publication date: July 20, 2017
    Applicant: OLYMPUS CORPORATION
    Inventor: Katsunori OGO
  • Secreted renilla luciferase
    Patent number: 6228604
    Abstract: A polynucleotide encoding a secreted form of wild type Renilla luciferase. Also provided is a polynucleotide encoding a secreted modified form of wild type Renilla luciferase. Additionally, the polypeptides encoded by the polynucleotides of the present invention and uses of the polynucleotides and polypeptides of the present invention in biological assays. Also, a stable mammalian packaging cell line which produces retroviruses carrying a polynucleotide encoding a secreted Renilla luciferase.
    Type: Grant
    Filed: June 10, 1999
    Date of Patent: May 8, 2001
    Assignee: Loma Linda University
    Inventors: Alan P. Escher, Jingxue Liu
  • CHIMERIC GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF
    Publication number: 20150259652
    Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which tyrosine at position of 138 is substituted with another amino acid and at least 3 amino acids selected from the group consisting of isoleucine at position of 90, proline at position of 115, glutamine at position of 124 and asparagine at position of 166 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.
    Type: Application
    Filed: March 9, 2015
    Publication date: September 17, 2015
    Inventors: Satoshi INOUYE, Junichi SATO
  • Multiplexed Luciferase Reporter Assay Systems
    Publication number: 20120102581
    Abstract: Inhibitors of luciferase enzymes are disclosed and find use in multiplexed assays using multiple luciferases and multiple inhibitors, in both in vitro and in vivo embodiments.
    Type: Application
    Filed: October 20, 2010
    Publication date: April 26, 2012
    Inventors: Lawrence Lum, Ozlem Kulak
  • SECRETED LUCIFERASE FOR EX VIVO MONITORING OF IN VIVO PROCESSES
    Publication number: 20090235370
    Abstract: The present invention generally relates to a methods, compositions and assays for real-time monitoring of the progression of a disease, such as a cancer in a subject, by measuring the level of bioluminescence signal in a biological sample obtained from a subject, where the bioluminescence signal is from a secreted luciferase protein expressed by a cell or tissue in the subject. One aspect of the present invention relates to administering to a subject a nucleic acid encoding a secreted luciferase, and in some embodiments, a disease or a diseased tissue such as tumor cells expresses the secreted luciferase protein, which is monitored in a biological sample, such as blood or urine obtained from a subject. One aspect of the invention relates to analysis of a secreted luciferase protein by measuring the level in a biological sample obtained from the subject without the need for invasive monitoring procedures.
    Type: Application
    Filed: January 16, 2009
    Publication date: September 17, 2009
    Applicant: THE GENERAL HOSPITAL CORPORATION
    Inventor: Bakhos A. Tannous
  • LUCIFERASE-BASED ASSAYS
    Publication number: 20150337359
    Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Application
    Filed: October 14, 2014
    Publication date: November 26, 2015
    Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
  • Photuris firefly luciferase gene
    Patent number: 5618722
    Abstract: Firefly luciferase gene for making use of the luminescence reaction of luciferase is provided.
    Type: Grant
    Filed: April 20, 1994
    Date of Patent: April 8, 1997
    Assignee: Chisso Corporation
    Inventors: Shuhei Zenno, Shinji Shiraishi, Satoshi Inouye, Kaoru Saigo
  • Luciferase and methods for measuring intracellular ATP using the same
    Publication number: 20040235077
    Abstract: The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.
    Type: Application
    Filed: April 22, 2004
    Publication date: November 25, 2004
    Applicant: Kikkoman Corporation
    Inventors: Noriaki Hattori, Seiji Murakami
  • Protease specific cleavable luciferases and methods of use thereof
    Patent number: 6890745
    Abstract: Provided are methods and compositions useful in detecting protease activity in a sample, as well as methods of identifying agents that modulate protease activity. The methods and compositions provide a modified luciferase polynucleotide sequence and a luciferase polypeptide containing protease recognition sequences, wherein cleavage of the recognition sequence by a protease inhibits luciferase activity. Further provided are methods and compositions for detecting and modulating caspase activity and apoptosis.
    Type: Grant
    Filed: July 19, 2000
    Date of Patent: May 10, 2005
    Assignee: Chemicon International, Inc.
    Inventor: Jay Leng
  • NOVEL LUCIFERASE SEQUENCES UTILIZING INFRARED-EMITTING SUBSTRATES TO PRODUCE ENHANCED LUMINESCENCE
    Publication number: 20160076079
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Application
    Filed: September 11, 2015
    Publication date: March 17, 2016
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
    Patent number: 9732373
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: August 15, 2017
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • LUCIFERASE-BASED ASSAYS
    Publication number: 20110081670
    Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Application
    Filed: June 21, 2010
    Publication date: April 7, 2011
    Applicant: PROMEGA CORPORATION
    Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
  • Luciferase and methods for measuring intracellular ATP using the same
    Patent number: 7560245
    Abstract: The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.
    Type: Grant
    Filed: April 22, 2004
    Date of Patent: July 14, 2009
    Assignee: Kikkoman Corporation
    Inventors: Noriaki Hattori, Seiji Murakami
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