Search Patents
  • NOVEL LUCIFERASE SEQUENCES UTILIZING INFRARED-EMITTING SUBSTRATES TO PRODUCE ENHANCED LUMINESCENCE
    Publication number: 20170314061
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Application
    Filed: July 10, 2017
    Publication date: November 2, 2017
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • Luciferase-based assays
    Patent number: 9631225
    Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: April 25, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
  • NOVEL LUCIFERASE SEQUENCES UTILIZING INFRARED-EMITTING SUBSTRATES TO PRODUCE ENHANCED LUMINESCENCE
    Publication number: 20200115735
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Application
    Filed: December 23, 2019
    Publication date: April 16, 2020
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
    Patent number: 10550420
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Grant
    Filed: July 10, 2017
    Date of Patent: February 4, 2020
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • NOVEL LUCIFERASE SEQUENCES UTILIZING INFRARED-EMITTING SUBSTRATES TO PRODUCE ENHANCED LUMINESCENCE
    Publication number: 20220186287
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Application
    Filed: March 1, 2022
    Publication date: June 16, 2022
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • LUCIFERASE DERIVED FROM LUCIDINA ACCENSA
    Publication number: 20140080199
    Abstract: An object of the invention is to provide a novel and useful luciferase. The luciferase according to the embodiments of the invention is derived from Lucidina accensa.
    Type: Application
    Filed: September 11, 2013
    Publication date: March 20, 2014
    Applicant: OLYMPUS CORPORATION
    Inventors: Katsunori OGO, Ryutaro AKIYOSHI, Mariko MURAI, Takashi KINEBUCHI, Hirobumi SUZUKI
  • Luciferase detection reagent kits and luciferase detection method using the kit
    Publication number: 20040043439
    Abstract: The object of the present invention is to provide a detection method and a method of manufacturing a detection kit which are characterized by use of an organic sulfur reagent, are effective at low concentration of the reagent, are inexpensive and have reduced unpleasant odor. Disclosed is a reagent kit for detecting a Coleoptera luciferase, comprising an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion. A method for detecting a Coleoptera luciferase, comprising step 1 of mixing an aqueous solution containing an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion with a sample containing a Coleoptera luciferase to give a mixed solution and step 2 of measuring the light emitted in the mixed solution is also disclosed.
    Type: Application
    Filed: March 13, 2003
    Publication date: March 4, 2004
    Applicants: Toyo B-Net Co., Ltd., TOYO INK MANUFACTURING CO., LTD.
    Inventors: Masayuki Ryufuku, Hozumi Tanaka, Chie Suzuki
  • Luciferase detection assay system
    Publication number: 20070054342
    Abstract: The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
    Type: Application
    Filed: May 12, 2006
    Publication date: March 8, 2007
    Inventors: Harry Lune, Johan Bruggeman
  • Method for enhancing activity of luciferase with fluorescence activity
    Publication number: 20060234324
    Abstract: Methods for enhancing luminescence of a luciferase (BFP-aq) with fluorescence activity derived from a calcium-binding photoprotein are provided. To a luciferase solution with fluorescence activity that contains an apoprotein, a calcium-binding photoprotein, which is constituted such that a coelenteramide or an analog thereof is coordinated inside, a coelenterazine that is the luminescent substrate of the luciferase or an analog thereof and a compound (e.g., imidazole etc.) having the function of removing an —NH-proton of the pyrazine ring of the imidazopyrazine skeleton in the coelenterazine or the analog thereof are added.
    Type: Application
    Filed: March 30, 2006
    Publication date: October 19, 2006
    Applicant: CHISSO CORPORATION
    Inventors: Satoshi Inouye, Satoko Sasaki
  • Luciferase derived from lucidina accensa
    Patent number: 9328334
    Abstract: An object of the invention is to provide a novel and useful luciferase. The luciferase according to the embodiments of the invention is derived from Lucidina accensa.
    Type: Grant
    Filed: September 11, 2013
    Date of Patent: May 3, 2016
    Assignee: OLYMPUS CORPORATION
    Inventors: Katsunori Ogo, Ryutaro Akiyoshi, Mariko Murai, Takashi Kinebuchi, Hirobumi Suzuki
  • Luciferase-based assays
    Patent number: 8361739
    Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: January 29, 2013
    Assignee: Promega Corporation
    Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
  • Oplophorus luciferase subunits
    Patent number: 6544754
    Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector. Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein. These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.
    Type: Grant
    Filed: April 26, 2001
    Date of Patent: April 8, 2003
    Assignee: Chisso Corporation
    Inventor: Satoshi Inouye
  • Luciferase-based assays
    Patent number: 8859220
    Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Grant
    Filed: January 25, 2013
    Date of Patent: October 14, 2014
    Assignee: Promega Corporation
    Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
  • Luciferase and methods for assaying intracellular ATP by using the same
    Patent number: 6812012
    Abstract: The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.
    Type: Grant
    Filed: June 26, 2000
    Date of Patent: November 2, 2004
    Assignee: Kikkoman Corporation
    Inventors: Noriaki Hattori, Seiji Murakami
  • Secreted renilla luciferase
    Patent number: 6451549
    Abstract: A polynucleotide encoding a secreted form of wild type Renilla luciferase. Also provided is a polynucleotide encoding a secreted modified form of wild type Renilla luciferase. Additionally, the polypeptides encoded by the polynucleotides of the present invention and uses of the polynucleotides and polypeptides of the present invention in biological assays. Also, a stable mammalian packaging cell line which produces retroviruses carrying a polynucleotide encoding a secreted Renilla luciferase.
    Type: Grant
    Filed: March 14, 2001
    Date of Patent: September 17, 2002
    Assignee: Loma Linda University
    Inventors: Alan P. Escher, Jingxue Liu
  • Modified railroad worm red luciferase coding sequences
    Publication number: 20030135871
    Abstract: Native and modified forms of Phrixothrix hirtus red luciferase are described. These native and modified forms of luciferase can be used, for example, as reporter molecules in host cells and/or transgenic animals.
    Type: Application
    Filed: August 15, 2002
    Publication date: July 17, 2003
    Inventors: Kevin A. Nawotka, Weisheng Zhang
  • Luciferase-based assays
    Patent number: 8603767
    Abstract: A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent.
    Type: Grant
    Filed: January 25, 2013
    Date of Patent: December 10, 2013
    Assignee: Promega Corporation
    Inventors: Erika Hawkins, James J. Cali, Samuel Kin Sang Ho, Martha A. O'Brien, Richard Somberg, Robert F. Bulleit, Keith V. Wood
  • TRANSGENIC ANIMAL OVEREXPRESSING LUCIFERASE AND PREPARATION METHOD THEREOF
    Publication number: 20120278911
    Abstract: The present disclosure provides a vector comprising a promoter and a luciferase gene having a nucleic acid sequence as disclosed in SEQ ID NO: 1; a fertilized egg transformed with the present vector; and a transgenic non-human animal overexpressing a luciferase gene from the vector and a method for preparing it. The vector and the animal of the present disclosure have a high expression rate for the luciferase gene, which confers high sensitivity for detection and thus useful for imaging analysis in a variety of research areas.
    Type: Application
    Filed: April 26, 2012
    Publication date: November 1, 2012
    Applicant: SNU R&DB Foundation
    Inventors: Eun Young CHOI, Hye Won Youn
  • Modified renilla luciferase nucleic acids and methods of use
    Patent number: 9006405
    Abstract: This invention provides modified nucleotide sequences encoding luciferase that have greater expression than wild type luciferase.
    Type: Grant
    Filed: May 27, 2011
    Date of Patent: April 14, 2015
    Assignee: SwitchGear Genomics, Inc.
    Inventors: Nathan D. Trinklein, Shelley Force Aldred
  • Construction and expression of renilla luciferase and green fluorescent protein fusion genes
    Patent number: 5976796
    Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.
    Type: Grant
    Filed: December 23, 1996
    Date of Patent: November 2, 1999
    Assignee: Loma Linda University
    Inventors: Aladar A. Szalay, Gefu Wang, Yubao Wang
Narrow Results

Filter by US Classification