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  • Method for detection of presence of target polynucleotide in samples
    Patent number: 8247171
    Abstract: A method for detecting the presence of a target polynucleotide in a sample, including providing a mixture of the sample and target binding agent and measuring a signal from the mixture, where the target binding agent is capable of assuming a first position where the target binding agent is not bound to the target polynucleotide and a second position where the target binding agent is bound to the target polynucleotide, and the intensity of the signal depends on the proportion of target binding agent in the first and second positions. The method is suitable for detecting a PCR product using a homogeneous detection method.
    Type: Grant
    Filed: February 1, 2008
    Date of Patent: August 21, 2012
    Assignee: Abacus Diagnostica Oy
    Inventors: Jussi Nurmi, Anniina Syrjälä, Piia Von Lode, Virve Hagren
  • METHOD FOR DETECTING NUCLEIC ACIDS
    Publication number: 20140017689
    Abstract: Method for detecting nucleic acids which employs a double-stranded oligonucleotide probe containing i) a first probe including a first label moiety, and ii) a second probe partially complementary with the first probe and including a second label moiety capable of interacting with the first moiety when brought in close proximity with each other, the second moiety being a quencher or acceptor of emission of the first moiety. The first or second probe includes a sequence complementary to that of a target nucleotide, and the second or first probe, respectively, includes a sequence complementary to a complement of the target nucleotide sequence of the nucleic acid to be detected. Oligonucleotides for determining Chlamydia trachomatis are also disclosed.
    Type: Application
    Filed: September 3, 2013
    Publication date: January 16, 2014
    Applicant: ABACUS DIAGNOSTICA OY
    Inventors: Piia VON LODE, Anniina WESTER, Timo LOVGREN, Tero SOUKKA
  • Temperature control of reaction vessel, system with reaction vessel, software product for system and use of system
    Patent number: 7754473
    Abstract: A method for rapid thermal control of a reaction volume from a preceding temperature to a target temperature includes first bringing at least the reaction vessel's second wall, which has high thermal conductivity, into direct contact with a first thermal block at a temperature higher than the target temperature if the target temperature is higher than the preceding temperature, or at a temperature lower than the target temperature if the target temperature is lower than the preceding temperature, until the reaction volume temperature is at least close to the target temperature; and then bringing the second wall into direct contact with a second thermal block at the target temperature. Also disclosed is a system (20) for detecting and/or quantitating a biological and/or chemical analyte in a sample and a software product for the system.
    Type: Grant
    Filed: June 3, 2005
    Date of Patent: July 13, 2010
    Assignee: Abacus Diagnostica Oy
    Inventors: Jussi Nurmi, Teemu Korpimäki, Timo Lövgren, Hannu Kojola, Antti Iitia
  • Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof
    Patent number: 7972838
    Abstract: A reagent container having an inner surface upon which at least two reagents are dried, with the first reagent dried on a first area separate from a second area where the second reagent is dried. The first and second reagents are a nucleic acid polymerase and its substrate. A method is disclosed which includes dispensing at least the first and second reagents onto separate areas of the inner surface of the reagent container, and removing excess water from the reagents. The reagent container can be used in a polymerase chain reaction (PCR) assay, an assay that utilizes a reverse transcriptase, a reverse transcriptase polymerase chain reaction, an immuno-PCR assay, a nucleic acid sequence based assay, a proximity ligation assay, a ligase chain reaction assay, a rolling circle amplification assay, and a strand displacement amplification assay.
    Type: Grant
    Filed: June 3, 2005
    Date of Patent: July 5, 2011
    Assignee: Abacus Diagnostica Oy
    Inventors: Teemu Korpimaki, Timo Lovgren, Jussi Nurmi
  • Luminescent lanthanide labelling reagents and their use
    Patent number: 8216856
    Abstract: The invention relates to a detectable molecule comprising a biospecific binding reactant attached to a luminescent lanthanide chelate comprising a lanthanide ion and a chelating ligand of the formula (I) wherein, R1A R1B are independently of each other selected from the group consisting of hydrogen, methyl, ethyl, —COOH, —COO—, —CH2COOH, —CH2COO—, hydroxyl or OR2; R2 is selected from the group consisting of —CH3, —C(CH3)3, —C(CR4)3, wherein R4 is an alkyl with 1 to 6 carbon atoms, —CH2COOH, —CH2COO?, and appropriate hexose residues; R3 is a linker for coupling to a biospecific binding reactant selected from the group consisting of thiourea (—NH—CS—NH—), aminoacetamide (—NH—CO—CH2—NH—), amide (—NH—CO— and —CO—NH—), aliphatic thioether (—S—), disulfide (—S—S—) and 6-substituted-1,3,5-triazine-2,4-diamine; and the lanthanide ion is selected from the group consisting of europium(III), terbium(III), dysprosium(III) and samarium(III). The invention also relates to corresponding lanthanide chelates.
    Type: Grant
    Filed: August 17, 2007
    Date of Patent: July 10, 2012
    Assignee: Abacus Diagnostica Oy
    Inventors: Harri Takalo, Jouko Kankare, Elina Tienari
  • LUMINESCENT LANTHANIDE LABELLING REAGENTS AND THEIR USE
    Publication number: 20100081211
    Abstract: The invention relates to a detectable molecule comprising a biospecific binding reactant attached to a luminescent lanthanide chelate comprising a lanthanide ion and a chelating ligand of the formula (I) wherein, R1A R1B are independently of each other selected from the group consisting of hydrogen, methyl, ethyl, —COOH, —COO—, —CH2COOH, —CH2COO—, hydroxyl or OR2; R2 is selected from the group consisting of —CH3, —C(CH3)3, —C(CR4)3, wherein R4 is an alkyl with 1 to 6 carbon atoms, —CH2COOH, —CH2COO?, and appropriate hexose residues; R3 is a linker for coupling to a biospecific binding reactant selected from the group consisting of thiourea (—NH—CS—NH—), aminoacetamide (—NH—CO—CH2—NH—), amide (—NH—CO— and —CO—NH—), aliphatic thioether (—S—), disulfide (—S—S—) and 6-substituted-1,3,5-triazine-2,4-diamine; and the lanthanide ion is selected from the group consisting of europium(III), terbium(III), dysprosium(III) and samarium(III). The invention also relates to corresponding lanthanide chelates.
    Type: Application
    Filed: August 17, 2007
    Publication date: April 1, 2010
    Applicant: ABACUS DIAGNOSTICA OY
    Inventors: Harri Takalo, Jouko Kankare, Elina Tienari
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