Search Patents
  • RECOMBINANT LECTIN AND USES THEREOF
    Publication number: 20190233479
    Abstract: Disclosed herein are a recombinant Streptomyces S27S5 hemagglutinin (SHA), and homologues thereof, and a fusion protein of a fluorescent protein (such as GFP and mCherry1) and SHA or a homologue thereof, which specifically bind to carbohydrates, including oligomeric sugars that terminate in L-rhamnose or D-galactose. The SHA, SHA homologues, and fusion proteins can be used to detect a variety of microorganisms or cancer or tumor antigens.
    Type: Application
    Filed: October 19, 2018
    Publication date: August 1, 2019
    Inventors: Markus KALKUM, Yoko FUJITA-YAMAGUCHI, Karine BAGRAMYAN, Teresa B. HONG, Sarah SHUCK
  • Recombinant lectin and uses thereof
    Patent number: 11767351
    Abstract: Disclosed herein are a recombinant Streptomyces S27S5 hemagglutinin (SHA), and homologues thereof, and a fusion protein of a fluorescent protein (such as GFP and mCherry1) and SHA or a homologue thereof, which specifically bind to carbohydrates, including oligomeric sugars that terminate in L-rhamnose or D-galactose. The SHA, SHA homologues, and fusion proteins can be used to detect a variety of microorganisms or cancer or tumor antigens.
    Type: Grant
    Filed: August 4, 2021
    Date of Patent: September 26, 2023
    Assignee: CITY OF HOPE
    Inventors: Markus Kalkum, Yoko Fujita-Yamaguchi, Karine Bagramyan, Teresa B. Hong, Sarah Shuck
  • RECOMBINANT LECTIN AND USES THEREOF
    Publication number: 20210395315
    Abstract: Disclosed herein are a recombinant Streptomyces S27S5 hemagglutinin (SHA), and homologues thereof, and a fusion protein of a fluorescent protein (such as GFP and mCherry1) and SHA or a homologue thereof, which specifically bind to carbohydrates, including oligomeric sugars that terminate in L-rhamnose or D-galactose. The SHA, SHA homologues, and fusion proteins can be used to detect a variety of microorganisms or cancer or tumor antigens.
    Type: Application
    Filed: August 4, 2021
    Publication date: December 23, 2021
    Applicant: CITY OF HOPE
    Inventors: Markus KALKUM, Yoko FUJITA-YAMAGUCHI, Karine BAGRAMYAN, Teresa B. HONG, Sarah SHUCK
  • METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF ASPERGILLOSIS
    Publication number: 20170038383
    Abstract: Provided herein are methods for detecting an Aspergillus protease in a sample, diagnosing a subject with aspergillosis caused by an Aspergillus infection based on the presence of an Aspergillus protease in a sample, and methods of aspergillosis treatment that incorporate these diagnostic methods. In certain embodiments, the Aspergillus protease is Asp f2, and the Aspergillus infection is caused A. fumigatus, A. flavus, A. versicolor, A. niger, or A. terreus. Also provided herein are antibodies and kits for use in these methods, including novel antibodies specific for Asp f2.
    Type: Application
    Filed: August 15, 2016
    Publication date: February 9, 2017
    Inventors: Markus KALKUM, Karine BAGRAMYAN, Diana DIAZ-AREVALO, James I. ITO, Sanjeet DADWAL
  • METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF ASPERGILLOSIS
    Publication number: 20140274882
    Abstract: Provided herein are methods for detecting an Aspergillus protease in a sample, diagnosing a subject with aspergillosis caused by an Aspergillus infection based on the presence of an Aspergillus protease in a sample, and methods of aspergillosis treatment that incorporate these diagnostic methods. In certain embodiments, the Aspergillus protease is Asp f2, and the Aspergillus infection is caused A. fumigatus, A. flavus, A. versicolor, A. niger, or A. terreus. Also provided herein are antibodies and kits for use in these methods, including novel antibodies specific for Asp f2.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Inventors: Markus KALKUM, Karine BAGRAMYAN, Diana DIAZ-AREVALO, James I. ITO, Sanjeet DADWAL
  • METHODS, COMPOSITIONS, AND KITS FOR DETECTION OF ASPERGILLOSIS
    Publication number: 20200271651
    Abstract: Provided herein are methods for detecting an Aspergillus protease in a sample, diagnosing a subject with aspergillosis caused by an Aspergillus infection based on the presence of an Aspergillus protease in a sample, and methods of aspergillosis treatment that incorporate these diagnostic methods. In certain embodiments, the Aspergillus protease is Asp f2, and the Aspergillus infection is caused A. fumigatus, A. flavus, A. versicolor, A. niger, or A. terreus. Also provided herein are antibodies and kits for use in these methods, including novel antibodies specific for Asp f2.
    Type: Application
    Filed: May 11, 2020
    Publication date: August 27, 2020
    Applicant: CITY OF HOPE
    Inventors: Markus KALKUM, Karine BAGRAMYAN, Diana DIAZ-AREVALO, James I. ITO, Sanjeet DADWAL
  • Methods, compositions, and kits for detection of aspergillosis
    Patent number: 11726087
    Abstract: Provided herein are methods for detecting an Aspergillus protease in a sample, diagnosing a subject with aspergillosis caused by an Aspergillus infection based on the presence of an Aspergillus protease in a sample, and methods of aspergillosis treatment that incorporate these diagnostic methods. In certain embodiments, the Aspergillus protease is Asp f2, and the Aspergillus infection is caused A. fumigatus, A. flavus, A. versicolor, A. niger, or A. terreus. Also provided herein are antibodies and kits for use in these methods, including novel antibodies specific for Asp f2.
    Type: Grant
    Filed: May 11, 2020
    Date of Patent: August 15, 2023
    Assignee: CITY OF HOPE
    Inventors: Markus Kalkum, Karine Bagramyan, Diana Diaz-Arevalo, James I. Ito, Sanjeet Dadwal
  • SINGLE CHAIN ANTIBODY DOMAINS FOR DETECTION OF ANTI-BOTULINUM NEUROTOXIN DOMAINS AND METHODS OF THEIR USE
    Publication number: 20180237507
    Abstract: Provided herein, inter alia, are single chain antibody binding domains (VHHs) for use in conjunction with the Assay with a Large Immuno-sorbent Surface Area (ALISSA). Engineered and affinity matured VHHs are used as affinity reagents in the ALISSA resulting in an exceptionally sensitive and precise method for the detection of toxins at miniscule concentrations. Thus, provided herein are methods as well as reagents that can be used to detect the presence of botulinum neurotoxins in quantities well below 1 pg/mL which corresponds to a lethal concentration under presumed equal distribution throughout the human body.
    Type: Application
    Filed: February 29, 2016
    Publication date: August 23, 2018
    Inventors: Markus KALKUM, Karine BAGRAMYAN, Paul YAZAKI
  • Methods, compositions, and kits for detection of aspergillosis
    Patent number: 10648982
    Abstract: Provided herein are methods for detecting an Aspergillus protease in a sample, diagnosing a subject with aspergillosis caused by an Aspergillus infection based on the presence of an Aspergillus protease in a sample, and methods of aspergillosis treatment that incorporate these diagnostic methods. In certain embodiments, the Aspergillus protease is Asp f2, and the Aspergillus infection is caused A. fumigatus, A. flavus, A. versicolor, A. niger, or A. terreus. Also provided herein are antibodies and kits for use in these methods, including novel antibodies specific for Asp f2.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: May 12, 2020
    Assignee: CITY OF HOPE
    Inventors: Markus Kalkum, Karine Bagramyan, Diana Diaz-Arevalo, James I. Ito, Sanjeet Dadwal
  • METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
    Publication number: 20120322081
    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorigenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.
    Type: Application
    Filed: November 29, 2011
    Publication date: December 20, 2012
    Inventors: Markus Kalkum, Karine Bagramyan
  • Single chain antibody domains for detection of anti-botulinum neurotoxin domains and methods of their use
    Patent number: 10479826
    Abstract: Provided herein, inter alia, are single chain antibody binding domains (VHHs) for use in conjunction with the Assay with a Large Immuno-sorbent Surface Area (ALISSA). Engineered and affinity matured VHHs are used as affinity reagents in the ALISSA resulting in an exceptionally sensitive and precise method for the detection of toxins at miniscule concentrations. Thus, provided herein are methods as well as reagents that can be used to detect the presence of botulinum neurotoxins in quantities well below 1 pg/mL which corresponds to a lethal concentration under presumed equal distribution throughout the human body.
    Type: Grant
    Filed: February 29, 2016
    Date of Patent: November 19, 2019
    Assignee: CITY OF HOPE
    Inventors: Markus Kalkum, Karine Bagramyan, Paul Yazaki
  • METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
    Publication number: 20090176259
    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorigenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.
    Type: Application
    Filed: June 5, 2008
    Publication date: July 9, 2009
    Inventors: Markus Kalkum, Karine Bagramyan
  • METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
    Publication number: 20170153228
    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.
    Type: Application
    Filed: February 6, 2017
    Publication date: June 1, 2017
    Inventors: Markus KALKUM, Karine BAGRAMYAN
  • Methods, compositions, and kits for detection of aspergillosis
    Patent number: 9416395
    Abstract: Provided herein are methods for detecting an Aspergillus protease in a sample, diagnosing a subject with aspergillosis caused by an Aspergillus infection based on the presence of an Aspergillus protease in a sample, and methods of aspergillosis treatment that incorporate these diagnostic methods. In certain embodiments, the Aspergillus protease is Asp f2, and the Aspergillus infection is caused A. fumigatus, A. flavus, A. versicolor, A. niger, or A. terreus. Also provided herein are antibodies and kits for use in these methods, including novel antibodies specific for Asp f2.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: August 16, 2016
    Assignee: CITY OF HOPE
    Inventors: Markus Kalkum, Karine Bagramyan, Diana Diaz-Arevalo, James I. Ito, Sanjeet Dadwal
  • METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
    Publication number: 20210255174
    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.
    Type: Application
    Filed: September 14, 2020
    Publication date: August 19, 2021
    Applicant: CITY OF HOPE
    Inventors: Markus KALKUM, Karine BAGRAMYAN
  • METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
    Publication number: 20130065259
    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.
    Type: Application
    Filed: August 30, 2012
    Publication date: March 14, 2013
    Applicant: CITY OF HOPE
    Inventors: Markus KALKUM, Karine BAGRAMYAN
  • Methods for detection of botulinum neurotoxin
    Patent number: 8067192
    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.
    Type: Grant
    Filed: June 5, 2008
    Date of Patent: November 29, 2011
    Assignee: City of Hope
    Inventors: Markus Kalkum, Karine Bagramyan
  • Substrates for detection of botulinum neurotoxin
    Patent number: 10775366
    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: September 15, 2020
    Assignee: CITY OF HOPE
    Inventors: Markus Kalkum, Karine Bagramyan
  • Methods for detection of botulinum neurotoxin
    Patent number: 9562903
    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.
    Type: Grant
    Filed: May 7, 2014
    Date of Patent: February 7, 2017
    Assignee: CITY OF HOPE
    Inventors: Markus Kalkum, Karine Bagramyan
  • Methods for detection of botulinum neurotoxin
    Patent number: 11604185
    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.
    Type: Grant
    Filed: September 14, 2020
    Date of Patent: March 14, 2023
    Assignee: CITY OF HOPE
    Inventors: Markus Kalkum, Karine Bagramyan
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